AlfalfaGEDB | Msa1169470

M. sativa cultivar zhongmu-4 / Msa1169470

KEGG: K12896
GO: GO:0000245 GO:0000375 GO:0000377 GO:0000398 GO:0002244 GO:0002376 GO:0002520 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0008380 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010629 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016070 GO:0016071 GO:0016604 GO:0016607 GO:0019219 GO:0019222 GO:0019904 GO:0022607 GO:0022613 GO:0022618 GO:0030097 GO:0030154 GO:0031123 GO:0031124 GO:0031323 GO:0031324 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0033036 GO:0033119 GO:0034613 GO:0034622 GO:0034641 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043484 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0045934 GO:0046483 GO:0046907 GO:0048024 GO:0048025 GO:0048513 GO:0048519 GO:0048523 GO:0048534 GO:0048731 GO:0048856 GO:0048869 GO:0050657 GO:0050658 GO:0050684 GO:0050686 GO:0050789 GO:0050794 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051172 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051253 GO:0051641 GO:0051649 GO:0060255 GO:0065003 GO:0065007 GO:0070013 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071826 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1903311 GO:1903312 GO:1990825
PFAM: PF00076.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1169470	XP_024625911.1	98.371	307	3	1	1	307	1	305	0.0	572

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1169470	sp\|Q9FYB7\|RSZ32_ARATH	64.706	255	66	5	1	238	1	248	3.49e-85	260

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1169470	A0A396HBT0	98.371	307	3	1	1	307	1	305	0.0	572

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003030	Msa1169470	0.809527	1.810468e-50	-8.615850e-47
Msa0146910	Msa1169470	0.808107	3.644063e-50	-8.615850e-47
Msa0376850	Msa1169470	0.843205	1.616669e-58	-8.615850e-47
Msa0516830	Msa1169470	0.841941	3.501469e-58	-8.615850e-47
Msa0691760	Msa1169470	0.807989	3.859398e-50	-8.615850e-47
Msa1078480	Msa1169470	0.919352	5.012534e-87	-8.615850e-47
Msa1078640	Msa1169470	0.929026	1.253124e-92	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1169470	MtrunA17_Chr7g0263341	98.371	307	3	1	1	307	1	305	0.0	572
Msa1169470	MtrunA17_Chr1g0192221	51.190	84	35	2	13	91	40	122	9.18e-16	76.3
Msa1169470	MtrunA17_Chr7g0269331	46.429	84	39	2	13	91	9	91	1.31e-12	67.0
Msa1169470	MtrunA17_Chr8g0357591	33.621	116	61	4	13	120	245	352	2.05e-11	64.7
Msa1169470	MtrunA17_Chr7g0272311	44.595	74	36	1	13	81	9	82	8.13e-11	61.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1169470	AT3G53500.2	64.706	255	66	5	1	238	1	248	3.55e-86	260
Msa1169470	AT2G37340.1	81.457	151	22	1	1	151	1	145	6.10e-86	260
Msa1169470	AT2G37340.5	78.182	110	18	1	42	151	1	104	1.38e-57	186
Msa1169470	AT2G37340.3	78.182	110	18	1	42	151	1	104	1.38e-57	186
Msa1169470	AT3G53500.1	75.000	120	23	2	42	160	1	114	1.88e-57	185
Msa1169470	AT3G53500.3	75.000	120	23	2	42	160	1	114	1.88e-57	185
Msa1169470	AT2G37340.2	77.451	102	17	1	50	151	20	115	1.90e-51	171
Msa1169470	AT2G37340.6	77.451	102	17	1	50	151	20	115	1.90e-51	171
Msa1169470	AT2G37340.4	77.451	102	17	1	50	151	20	115	1.90e-51	171
Msa1169470	AT4G02430.1	50.000	82	36	1	13	89	9	90	1.05e-15	74.3

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Find 130 sgRNAs with CRISPR-Local

Find 334 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCTAACCCTTTGAAAG+TGG	0.491426	7_4:-62518004	None:intergenic
AACAAAACATATGAATATCC+TGG	0.516284	7_4:-62521657	None:intergenic
AAGAACAAGTGTTACCGCTG+TGG	0.645882	7_4:+62520831	Msa1169470:CDS
AAGACAAACGACCCACATAG+AGG	0.604217	7_4:-62518471	None:intergenic
AAGATAGCCCTAAGGCTAAT+GGG	0.465291	7_4:+62521499	Msa1169470:CDS
AAGCAGCGTCCAGATCCAGG+AGG	0.786991	7_4:-62520756	None:intergenic
AAGCCGCAGCCGCAGCCCCA+GGG	0.571588	7_4:+62521536	Msa1169470:CDS
AAGTATAGTCGATCTCCTGA+AGG	0.670998	7_4:+62521363	Msa1169470:CDS
AATAAGGATTGGTACCTTGG+AGG	0.577956	7_4:-62518036	None:intergenic
AATGAGCTCAGGACTCGGCC+AGG	0.524548	7_4:+62521639	Msa1169470:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GATATAAATAGTGAAACGCA+TGG	+	chr7_4:62517969-62517988	Msa1169470:five_prime_UTR	30.0%
TTGGGAATGGAATGATTAAA+GGG	-	chr7_4:62518168-62518187	None:intergenic	30.0%
ATTGGGAATGGAATGATTAA+AGG	-	chr7_4:62518169-62518188	None:intergenic	30.0%
TTTCATAAGTTCTCCAACAT+AGG	-	chr7_4:62519018-62519037	None:intergenic	30.0%
TTCCCCTAACTTTAGATATA+GGG	-	chr7_4:62519153-62519172	None:intergenic	30.0%
TTTCCCCTAACTTTAGATAT+AGG	-	chr7_4:62519154-62519173	None:intergenic	30.0%
AAAGTTAGGGGAAAATATAC+AGG	+	chr7_4:62519160-62519179	Msa1169470:intron	30.0%
AAGTTAAAAATGACACCTGT+GGG	-	chr7_4:62519256-62519275	None:intergenic	30.0%
TAAGTTAAAAATGACACCTG+TGG	-	chr7_4:62519257-62519276	None:intergenic	30.0%
TTCCAGTAGTTAAGTAATGA+TGG	+	chr7_4:62519491-62519510	Msa1169470:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_4	gene	62517957	62521953	62517957	ID=Msa1169470;Name=Msa1169470
chr7_4	mRNA	62517957	62521953	62517957	ID=Msa1169470-mRNA-1;Parent=Msa1169470;Name=Msa1169470-mRNA-1;_AED=0.23;_eAED=0.23;_QI=103\|1\|1\|1\|0.83\|0.71\|7\|333\|307
chr7_4	exon	62517957	62518043	62517957	ID=Msa1169470-mRNA-1:exon:19837;Parent=Msa1169470-mRNA-1
chr7_4	exon	62518420	62518542	62518420	ID=Msa1169470-mRNA-1:exon:19838;Parent=Msa1169470-mRNA-1
chr7_4	exon	62519544	62519583	62519544	ID=Msa1169470-mRNA-1:exon:19839;Parent=Msa1169470-mRNA-1
chr7_4	exon	62519749	62519844	62519749	ID=Msa1169470-mRNA-1:exon:19840;Parent=Msa1169470-mRNA-1
chr7_4	exon	62520699	62520904	62520699	ID=Msa1169470-mRNA-1:exon:19841;Parent=Msa1169470-mRNA-1
chr7_4	exon	62521038	62521172	62521038	ID=Msa1169470-mRNA-1:exon:19842;Parent=Msa1169470-mRNA-1
chr7_4	exon	62521281	62521953	62521281	ID=Msa1169470-mRNA-1:exon:19843;Parent=Msa1169470-mRNA-1
chr7_4	five_prime_UTR	62517957	62518043	62517957	ID=Msa1169470-mRNA-1:five_prime_utr;Parent=Msa1169470-mRNA-1
chr7_4	five_prime_UTR	62518420	62518435	62518420	ID=Msa1169470-mRNA-1:five_prime_utr;Parent=Msa1169470-mRNA-1
chr7_4	CDS	62518436	62518542	62518436	ID=Msa1169470-mRNA-1:cds;Parent=Msa1169470-mRNA-1
chr7_4	CDS	62519544	62519583	62519544	ID=Msa1169470-mRNA-1:cds;Parent=Msa1169470-mRNA-1
chr7_4	CDS	62519749	62519844	62519749	ID=Msa1169470-mRNA-1:cds;Parent=Msa1169470-mRNA-1
chr7_4	CDS	62520699	62520904	62520699	ID=Msa1169470-mRNA-1:cds;Parent=Msa1169470-mRNA-1
chr7_4	CDS	62521038	62521172	62521038	ID=Msa1169470-mRNA-1:cds;Parent=Msa1169470-mRNA-1
chr7_4	CDS	62521281	62521620	62521281	ID=Msa1169470-mRNA-1:cds;Parent=Msa1169470-mRNA-1
chr7_4	three_prime_UTR	62521621	62521953	62521621	ID=Msa1169470-mRNA-1:three_prime_utr;Parent=Msa1169470-mRNA-1

Gene Sequence

>Msa1169470

ATGCCTCGCTACGATGATAAGTATGGCAATACTCGCCTCTATGTGGGTCGTTTGTCTTCACGAACTCGTTCCCGTGATCTGGAGCGGGTCTTCAGCAGATATGGAAGCGTTCGAGATGTGGATATGAAGCATGATTACGCCTTTGTTGAATTTAGAGATCCTCGAGATGCTGATGATGCAAGATACAATTTGGATGGTCGCGATATTGATGGAAGTCGTCTTATTGTGGAATTTGCAAAAGGGGTCCCTCGGGGTTCCCGTGATTCTCGGGATTCTAGGGAATATTTGGGTCGAGGTCCACCTCCTGGATCTGGACGCTGCTTTAACTGTGGCATTGATGGCCACTGGGCTCGAGATTGCAAAGCTGGGGATTGGAAGAACAAGTGTTACCGCTGTGGGGAGAGAGGTCATATCGAAAAGAACTGCAAGAACAGTCCAAAAAAATTGAGCAGACATGCACGAAGTGTTTCACGTTCTCCAGGCAGGTCACGTTCTCCAGCCAGGTCTCGTTCTCCAGCCAGGTCTCGTTCTCCTAGGAGAGGCAGAAGCCGTGACAGAAGCTACAGCCCGGCTCGCAGCTATAGCCGATCAAGATCTCCTGTCAGAAGAGATCGAAGCCCAGTTCCTTCAGATAGATCACGGAGTCCTCCGCCTTCCAAGTCAAGGAAGTATAGTCGATCTCCTGAAGGCAGTCCACAAAAGAGCGCATCTCCTGGGAATGATAGGGTGGTTGCAACCCAAGATGGGTCTGATTACAGTGATGGTGCTAGAGTGAAGAGCAGAAGCCCCTCAAGGGACAATGAAGATAGCCCTAAGGCTAATGGGCGCAGCAGAAGCCGAAGCCGCAGCCGCAGCCCCAGGGATGAGGATAGGAGCCCCGTAGAGGAGGATGATGACAACAATCGTCGCTCTCCATCACCTTAA

Protein sequence

>Msa1169470

MPRYDDKYGNTRLYVGRLSSRTRSRDLERVFSRYGSVRDVDMKHDYAFVEFRDPRDADDARYNLDGRDIDGSRLIVEFAKGVPRGSRDSRDSREYLGRGPPPGSGRCFNCGIDGHWARDCKAGDWKNKCYRCGERGHIEKNCKNSPKKLSRHARSVSRSPGRSRSPARSRSPARSRSPRRGRSRDRSYSPARSYSRSRSPVRRDRSPVPSDRSRSPPPSKSRKYSRSPEGSPQKSASPGNDRVVATQDGSDYSDGARVKSRSPSRDNEDSPKANGRSRSRSRSRSPRDEDRSPVEEDDDNNRRSPSP*