AlfalfaGEDB | Msa0376850

M. sativa cultivar zhongmu-4 / Msa0376850

KEGG: K02942
GO: GO:0001932 GO:0001934 GO:0002181 GO:0003674 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005840 GO:0005844 GO:0005886 GO:0006412 GO:0006518 GO:0006807 GO:0008047 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009893 GO:0009987 GO:0010467 GO:0010562 GO:0010604 GO:0015934 GO:0016020 GO:0019207 GO:0019209 GO:0019220 GO:0019222 GO:0019538 GO:0019887 GO:0022625 GO:0022626 GO:0030234 GO:0030295 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032991 GO:0033674 GO:0034641 GO:0034645 GO:0042325 GO:0042327 GO:0042788 GO:0043043 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043549 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0044877 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071944 GO:0080090 GO:0098772 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00428.22

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0376850	XP_003602368.1	99.091	110	1	0	1	110	1	110	2.69e-70	215

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0376850	sp\|P52855\|RLA1_MAIZE	70.909	110	31	1	1	110	1	109	3.81e-29	104

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0376850	G7J8Y6	99.091	110	1	0	1	110	1	110	1.28e-70	215

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001760	Msa0376850	0.822066	2.888996e-53	-8.615850e-47
Msa0003030	Msa0376850	0.857194	1.927092e-62	-8.615850e-47
Msa0003330	Msa0376850	0.827990	1.152982e-54	-8.615850e-47
Msa0027590	Msa0376850	0.813346	2.681591e-51	-8.615850e-47
Msa0042510	Msa0376850	0.860723	1.693047e-63	-8.615850e-47
Msa0079560	Msa0376850	0.849680	2.768002e-60	-8.615850e-47
Msa0127230	Msa0376850	0.842657	2.261528e-58	-8.615850e-47
Msa0146910	Msa0376850	0.853936	1.718652e-61	-8.615850e-47
Msa0167860	Msa0376850	0.843186	1.635526e-58	-8.615850e-47
Msa0168320	Msa0376850	0.874417	6.865129e-68	-8.615850e-47
Msa0169710	Msa0376850	0.823292	1.498380e-53	-8.615850e-47
Msa0183840	Msa0376850	0.845730	3.383613e-59	-8.615850e-47
Msa0191550	Msa0376850	0.811024	8.611199e-51	-8.615850e-47
Msa0192300	Msa0376850	0.864388	1.260482e-64	-8.615850e-47
Msa0195030	Msa0376850	0.855557	5.825730e-62	-8.615850e-47
Msa0233550	Msa0376850	0.853594	2.154746e-61	-8.615850e-47
Msa0237180	Msa0376850	0.844931	5.569469e-59	-8.615850e-47
Msa0371490	Msa0376850	0.836051	1.173295e-56	-8.615850e-47
Msa0373350	Msa0376850	0.860960	1.434708e-63	-8.615850e-47
Msa0376850	Msa0377370	0.836249	1.045165e-56	-8.615850e-47
Msa0376850	Msa0377520	0.815827	7.572109e-52	-8.615850e-47
Msa0376850	Msa0377610	0.830220	3.319654e-55	-8.615850e-47
Msa0376850	Msa0382670	0.817167	3.794411e-52	-8.615850e-47
Msa0376850	Msa0387290	0.814078	1.850476e-51	-8.615850e-47
Msa0376850	Msa0388520	0.839622	1.419316e-57	-8.615850e-47
Msa0376850	Msa0416470	0.828108	1.079737e-54	-8.615850e-47
Msa0376850	Msa0421920	0.834960	2.215891e-56	-8.615850e-47
Msa0376850	Msa0426940	0.828726	7.657249e-55	-8.615850e-47
Msa0376850	Msa0467740	0.812874	3.404100e-51	-8.615850e-47
Msa0376850	Msa0485220	0.806243	9.043910e-50	-8.615850e-47
Msa0376850	Msa0511760	0.863547	2.303559e-64	-8.615850e-47
Msa0376850	Msa0514130	0.813206	2.878006e-51	-8.615850e-47
Msa0376850	Msa0516830	0.999783	0.000000e+00	-8.615850e-47
Msa0376850	Msa0547920	0.803673	3.115456e-49	-8.615850e-47
Msa0376850	Msa0573550	0.866427	2.874155e-65	-8.615850e-47
Msa0376850	Msa0574780	0.858707	6.849965e-63	-8.615850e-47
Msa0376850	Msa0578010	0.823908	1.075210e-53	-8.615850e-47
Msa0376850	Msa0597240	0.814343	1.616582e-51	-8.615850e-47
Msa0376850	Msa0602900	0.809615	1.733222e-50	-8.615850e-47
Msa0376850	Msa0605890	0.859268	4.650903e-63	-8.615850e-47
Msa0376850	Msa0609120	0.859390	4.274347e-63	-8.615850e-47
Msa0376850	Msa0619230	0.885489	7.781743e-72	-8.615850e-47
Msa0376850	Msa0621190	0.879188	1.527976e-69	-8.615850e-47
Msa0376850	Msa0643960	0.809525	1.812724e-50	-8.615850e-47
Msa0376850	Msa0656450	0.873092	1.921505e-67	-8.615850e-47
Msa0376850	Msa0686550	0.819453	1.152245e-52	-8.615850e-47
Msa0376850	Msa0691760	0.898890	3.405499e-77	-8.615850e-47
Msa0376850	Msa0694400	0.809545	1.794751e-50	-8.615850e-47
Msa0376850	Msa0700370	0.832148	1.114944e-55	-8.615850e-47
Msa0376850	Msa0704860	0.837758	4.302731e-57	-8.615850e-47
Msa0376850	Msa0834220	0.808544	2.940370e-50	-8.615850e-47
Msa0376850	Msa0902730	0.810414	1.166517e-50	-8.615850e-47
Msa0376850	Msa0929700	0.848830	4.773107e-60	-8.615850e-47
Msa0376850	Msa0930360	0.828501	8.680628e-55	-8.615850e-47
Msa0376850	Msa0930790	0.846664	1.884767e-59	-8.615850e-47
Msa0376850	Msa1078480	0.818519	1.879441e-52	-8.615850e-47
Msa0376850	Msa1078640	0.828820	7.269887e-55	-8.615850e-47
Msa0376850	Msa1082050	0.820370	7.110028e-53	-8.615850e-47
Msa0376850	Msa1126690	0.814430	1.546629e-51	-8.615850e-47
Msa0376850	Msa1165700	0.801793	7.614402e-49	-8.615850e-47
Msa0376850	Msa1169470	0.843205	1.616669e-58	-8.615850e-47
Msa0376850	Msa1227350	0.848180	7.222966e-60	-8.615850e-47
Msa0376850	Msa1235950	0.834960	2.215891e-56	-8.615850e-47
Msa0376850	Msa1235980	0.839361	1.658888e-57	-8.615850e-47
Msa0376850	Msa1237040	0.809487	1.846671e-50	-8.615850e-47
Msa0376850	Msa1284800	0.821828	3.280022e-53	-8.615850e-47
Msa0376850	Msa1317390	0.823260	1.523967e-53	-8.615850e-47
Msa0376850	Msa1351020	0.863577	2.254351e-64	-8.615850e-47
Msa0376850	Msa1387750	0.860863	1.535431e-63	-8.615850e-47
Msa0376850	Msa1390760	0.887044	2.014995e-72	-8.615850e-47
Msa0376850	Msa1407550	0.869690	2.562534e-66	-8.615850e-47
Msa0376850	Msa1413570	0.873077	1.944067e-67	-8.615850e-47
Msa0376850	Msa1432310	0.864432	1.221147e-64	-8.615850e-47
Msa0376850	Msa1446510	0.866447	2.832126e-65	-8.615850e-47
Msa0376850	Msa1465700	0.840924	6.484716e-58	-8.615850e-47
Msa0376850	Msa1465890	0.804341	2.263246e-49	-8.615850e-47
Msa0239420	Msa0376850	0.859995	2.811330e-63	-8.615850e-47
Msa0256350	Msa0376850	0.812104	5.014056e-51	-8.615850e-47
Msa0274940	Msa0376850	0.868409	6.669495e-66	-8.615850e-47
Msa0281330	Msa0376850	0.807148	5.822513e-50	-8.615850e-47
Msa0281370	Msa0376850	0.843898	1.055596e-58	-8.615850e-47
Msa0301250	Msa0376850	0.859087	5.271179e-63	-8.615850e-47
Msa0313060	Msa0376850	0.874306	7.485721e-68	-8.615850e-47
Msa0315430	Msa0376850	0.816831	4.515186e-52	-8.615850e-47
Msa0315660	Msa0376850	0.852001	6.145286e-61	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0376850	MtrunA17_Chr3g0127831	99.091	110	1	0	1	110	1	110	2.47e-74	215
Msa0376850	MtrunA17_Chr2g0311301	98.182	110	2	0	1	110	1	110	8.45e-74	214
Msa0376850	MtrunA17_Chr4g0013621	91.818	110	9	0	1	110	1	110	2.10e-45	142
Msa0376850	MtrunA17_Chr8g0345951	64.545	110	37	2	1	110	1	108	1.12e-33	112
Msa0376850	MtrunA17_Chr7g0276131	62.712	59	22	0	1	59	1	59	7.89e-21	80.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0376850	AT5G24510.1	57.391	115	40	4	1	110	1	111	4.31e-36	119
Msa0376850	AT5G47700.1	57.658	111	45	1	2	110	3	113	8.30e-29	100
Msa0376850	AT5G47700.2	57.658	111	45	1	2	110	3	113	8.30e-29	100
Msa0376850	AT1G01100.4	60.185	108	42	1	4	110	5	112	4.43e-28	99.0
Msa0376850	AT1G01100.1	60.185	108	42	1	4	110	5	112	4.43e-28	99.0
Msa0376850	AT1G01100.2	60.185	108	42	1	4	110	5	112	4.43e-28	99.0
Msa0376850	AT1G01100.3	50.459	109	39	1	2	110	3	96	2.22e-27	96.7
Msa0376850	AT4G00810.1	54.955	111	48	1	2	110	3	113	2.98e-27	97.1
Msa0376850	AT4G00810.2	54.955	111	48	1	2	110	3	113	2.98e-27	97.1

Find 50 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATATCGAAACTTCACATTT+TGG	0.211931	3_1:-77172147	Msa0376850:five_prime_UTR
TTATAAAACAAAACAAGTTT+TGG	0.254235	3_1:+77170656	None:intergenic
TTCCCGCATCAGATGTAAAA+TGG	0.280734	3_1:+77170520	None:intergenic
CAAAATGAGTTCCGGCGAAT+TGG	0.294649	3_1:-77172073	Msa0376850:exon
GTCATCGTCACTTTCTTCCT+TGG	0.329724	3_1:+77170761	None:intergenic
AGAAACCTAGGTTAATTGAT+TGG	0.340250	3_1:+77172187	None:intergenic
ACGTCACCGTTGAATCTTAC+TGG	0.348075	3_1:-77171867	Msa0376850:CDS
AAAATGAGTTCCGGCGAATT+GGG	0.368280	3_1:-77172072	Msa0376850:CDS
TAGTTTGTTGTAGCCAGATT+AGG	0.371827	3_1:-77170703	Msa0376850:three_prime_UTR
CCTTGAAAGGAGTCCTAATC+TGG	0.373967	3_1:+77170690	None:intergenic
CCAGATTAGGACTCCTTTCA+AGG	0.391998	3_1:-77170690	Msa0376850:three_prime_UTR
TCTGAGCAAGCTTAGCGAAT+AGG	0.392998	3_1:+77171839	None:intergenic
GAAACCTAGGTTAATTGATT+GGG	0.394188	3_1:+77172188	None:intergenic
GATAATAGACTTACGGTGAT+TGG	0.399296	3_1:+77172009	None:intergenic
GCCGTATCTGCACCCGCTGC+TGG	0.401410	3_1:-77171760	Msa0376850:CDS
TCTGCACCCGCTGCTGGTGG+TGG	0.439771	3_1:-77171754	Msa0376850:CDS
ATTGTTCTGCCACACAAACT+AGG	0.448236	3_1:+77172105	None:intergenic
GCGGCTCCACCACCAGCAGC+GGG	0.451994	3_1:+77171748	None:intergenic
ATAATAGACTTACGGTGATT+GGG	0.452174	3_1:+77172010	None:intergenic
GAAGATCGGTACTTTGTTGA+AGG	0.461021	3_1:-77171896	Msa0376850:CDS
ACCTGCCGCCGTTGAAAAGA+AGG	0.476631	3_1:-77171713	Msa0376850:intron
TGCGGCTCCACCACCAGCAG+CGG	0.489051	3_1:+77171747	None:intergenic
TTGAACTCCGGCGCTGTTGG+TGG	0.510539	3_1:-77171793	Msa0376850:CDS
TGAAGTTTCGATATATATAG+TGG	0.520113	3_1:+77172155	None:intergenic
ACAATTCACAAAATGAGTTC+CGG	0.522809	3_1:-77172081	Msa0376850:exon
GGAAGAAAGTGACGATGACA+TGG	0.523361	3_1:-77170757	Msa0376850:CDS
AGCGGCACCACCAACAGCGC+CGG	0.527844	3_1:+77171786	None:intergenic
ACCAGCAGCGGGTGCAGATA+CGG	0.530473	3_1:+77171759	None:intergenic
GGATTCAGCCACTCCTTGAA+AGG	0.532068	3_1:+77170677	None:intergenic
TCCATCGTCGTGGAGGATCA+AGG	0.532980	3_1:+77172035	None:intergenic
GCAAGCTTAGCGAATAGGCT+TGG	0.542190	3_1:+77171844	None:intergenic
TTTGTTTAGGCGGAGAAGAT+CGG	0.543289	3_1:-77171910	Msa0376850:intron
GGCAGGTTCGGCAGCTGCTG+CGG	0.548908	3_1:+77171729	None:intergenic
TTGTTCTGCCACACAAACTA+GGG	0.550348	3_1:+77172106	None:intergenic
GTAAATGCAGCCCAATTCGC+CGG	0.571217	3_1:+77172062	None:intergenic
ACCTTGATCCTCCACGACGA+TGG	0.573503	3_1:-77172036	Msa0376850:CDS
AAGGCCCAATCAATTAACCT+AGG	0.578918	3_1:-77172192	Msa0376850:five_prime_UTR
TTAGGACTCCTTTCAAGGAG+TGG	0.585485	3_1:-77170685	Msa0376850:three_prime_UTR
TTCGTAAACCCTAGTTTGTG+TGG	0.589308	3_1:-77172114	Msa0376850:five_prime_UTR
AAAATCTTTCAAAATTAACC+TGG	0.590654	3_1:+77170488	None:intergenic
GTATCTGCACCCGCTGCTGG+TGG	0.603091	3_1:-77171757	Msa0376850:CDS
ATGCGGGAATATATCATACC+AGG	0.604298	3_1:-77170506	Msa0376850:three_prime_UTR
TGGCGTGTTATCAGAAACCT+AGG	0.605084	3_1:+77172175	None:intergenic
GCTTGGCCAGTAAGATTCAA+CGG	0.629260	3_1:+77171861	None:intergenic
TTGGGATTCCATCGTCGTGG+AGG	0.650788	3_1:+77172028	None:intergenic
GTGTTTGTAGGAGGAAGCCA+AGG	0.654949	3_1:-77170778	Msa0376850:intron
TGATTGGGATTCCATCGTCG+TGG	0.662981	3_1:+77172025	None:intergenic
GGGTGCAGATACGGCAACAG+CGG	0.665524	3_1:+77171768	None:intergenic
GAAGAAAGTGACGATGACAT+GGG	0.678372	3_1:-77170756	Msa0376850:CDS
ATCGTCGTGGAGGATCAAGG+TGG	0.743990	3_1:+77172038	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATTATATGTTTTTATCTTA+AGG	+	chr3_1:77171174-77171193	None:intergenic	10.0%
!!!	AATTATATGTTTTTATCTTA+AGG	+	chr3_1:77171174-77171193	None:intergenic	10.0%
!!	AATTGTATTGAAATTAGAAA+CGG	-	chr3_1:77171075-77171094	Msa0376850:intron	15.0%
!!	TATAAAAGATATAATTTCCT+CGG	-	chr3_1:77171297-77171316	Msa0376850:intron	15.0%
!!!	TTTTCATGCAAAATAATTTT+TGG	+	chr3_1:77171368-77171387	None:intergenic	15.0%
!!!	TTATAAAACAAAACAAGTTT+TGG	+	chr3_1:77172034-77172053	None:intergenic	15.0%
!!!	CTCATTTTATTGTTAAATTT+TGG	-	chr3_1:77172089-77172108	Msa0376850:exon	15.0%
!!	AATTGTATTGAAATTAGAAA+CGG	-	chr3_1:77171075-77171094	Msa0376850:intron	15.0%
!!	TATAAAAGATATAATTTCCT+CGG	-	chr3_1:77171297-77171316	Msa0376850:intron	15.0%
!!!	TTTTCATGCAAAATAATTTT+TGG	+	chr3_1:77171368-77171387	None:intergenic	15.0%
!!!	TTATAAAACAAAACAAGTTT+TGG	+	chr3_1:77172034-77172053	None:intergenic	15.0%
!!!	CTCATTTTATTGTTAAATTT+TGG	-	chr3_1:77172089-77172108	Msa0376850:exon	15.0%
!!!	ATTTTTCTTTTGTTACTGTT+TGG	-	chr3_1:77170747-77170766	Msa0376850:CDS	20.0%
!!!	TTTTTCTTTTGTTACTGTTT+GGG	-	chr3_1:77170748-77170767	Msa0376850:CDS	20.0%
!!	ATTGTATTGAAATTAGAAAC+GGG	-	chr3_1:77171076-77171095	Msa0376850:intron	20.0%
!!!	ATGAAAACATGTAGTTATTT+TGG	-	chr3_1:77171405-77171424	Msa0376850:intron	20.0%
!!!	TAATTGTTGTTTTTCTTCAT+TGG	-	chr3_1:77171446-77171465	Msa0376850:intron	20.0%
!!!	AATTGTTGTTTTTCTTCATT+GGG	-	chr3_1:77171447-77171466	Msa0376850:intron	20.0%
!!	AAGTAAAAAATGTGGATATA+GGG	+	chr3_1:77171736-77171755	None:intergenic	20.0%
!!	AAAGTAAAAAATGTGGATAT+AGG	+	chr3_1:77171737-77171756	None:intergenic	20.0%
!!!	ATATCCACATTTTTTACTTT+TGG	-	chr3_1:77171737-77171756	Msa0376850:CDS	20.0%
!!!	TATCCACATTTTTTACTTTT+GGG	-	chr3_1:77171738-77171757	Msa0376850:CDS	20.0%
!!!	ATTTTTCTTTTGTTACTGTT+TGG	-	chr3_1:77170747-77170766	Msa0376850:CDS	20.0%
!!!	TTTTTCTTTTGTTACTGTTT+GGG	-	chr3_1:77170748-77170767	Msa0376850:CDS	20.0%
!!	ATTGTATTGAAATTAGAAAC+GGG	-	chr3_1:77171076-77171095	Msa0376850:intron	20.0%
!!!	ATGAAAACATGTAGTTATTT+TGG	-	chr3_1:77171405-77171424	Msa0376850:intron	20.0%
!!!	TAATTGTTGTTTTTCTTCAT+TGG	-	chr3_1:77171446-77171465	Msa0376850:intron	20.0%
!!!	AATTGTTGTTTTTCTTCATT+GGG	-	chr3_1:77171447-77171466	Msa0376850:intron	20.0%
!!	AAGTAAAAAATGTGGATATA+GGG	+	chr3_1:77171736-77171755	None:intergenic	20.0%
!!	AAAGTAAAAAATGTGGATAT+AGG	+	chr3_1:77171737-77171756	None:intergenic	20.0%
!!!	ATATCCACATTTTTTACTTT+TGG	-	chr3_1:77171737-77171756	Msa0376850:CDS	20.0%
!!!	TATCCACATTTTTTACTTTT+GGG	-	chr3_1:77171738-77171757	Msa0376850:CDS	20.0%
!	TGAAGTTTCGATATATATAG+TGG	+	chr3_1:77170535-77170554	None:intergenic	25.0%
!!	TATATCGAAACTTCACATTT+TGG	-	chr3_1:77170540-77170559	Msa0376850:three_prime_UTR	25.0%
!	TATAGAAATGGAATTGAAGA+GGG	+	chr3_1:77170703-77170722	None:intergenic	25.0%
!	TTATAGAAATGGAATTGAAG+AGG	+	chr3_1:77170704-77170723	None:intergenic	25.0%
!	CGATTTGAAGATTATAGAAA+TGG	+	chr3_1:77170715-77170734	None:intergenic	25.0%
!!	AAACAATACCTTCTTTTCAA+CGG	+	chr3_1:77170985-77171004	None:intergenic	25.0%
!	GACAACTCTGAATATAATTA+TGG	-	chr3_1:77171497-77171516	Msa0376850:intron	25.0%
!!!	ATAAAGTTAACTTGTTTTCC+TGG	-	chr3_1:77171627-77171646	Msa0376850:intron	25.0%
!!!	TAAAGTTAACTTGTTTTCCT+GGG	-	chr3_1:77171628-77171647	Msa0376850:intron	25.0%
!	TTAAATAAGCCAATCATAAC+TGG	-	chr3_1:77171710-77171729	Msa0376850:intron	25.0%
!	TGAAGTTTCGATATATATAG+TGG	+	chr3_1:77170535-77170554	None:intergenic	25.0%
!!	TATATCGAAACTTCACATTT+TGG	-	chr3_1:77170540-77170559	Msa0376850:three_prime_UTR	25.0%
!	TATAGAAATGGAATTGAAGA+GGG	+	chr3_1:77170703-77170722	None:intergenic	25.0%
!	TTATAGAAATGGAATTGAAG+AGG	+	chr3_1:77170704-77170723	None:intergenic	25.0%
!	CGATTTGAAGATTATAGAAA+TGG	+	chr3_1:77170715-77170734	None:intergenic	25.0%
!!	AAACAATACCTTCTTTTCAA+CGG	+	chr3_1:77170985-77171004	None:intergenic	25.0%
!	GACAACTCTGAATATAATTA+TGG	-	chr3_1:77171497-77171516	Msa0376850:intron	25.0%
!!!	ATAAAGTTAACTTGTTTTCC+TGG	-	chr3_1:77171627-77171646	Msa0376850:intron	25.0%
!!!	TAAAGTTAACTTGTTTTCCT+GGG	-	chr3_1:77171628-77171647	Msa0376850:intron	25.0%
!	TTAAATAAGCCAATCATAAC+TGG	-	chr3_1:77171710-77171729	Msa0376850:intron	25.0%
	GAAACCTAGGTTAATTGATT+GGG	+	chr3_1:77170502-77170521	None:intergenic	30.0%
	AGAAACCTAGGTTAATTGAT+TGG	+	chr3_1:77170503-77170522	None:intergenic	30.0%
	ACAATTCACAAAATGAGTTC+CGG	-	chr3_1:77170606-77170625	Msa0376850:three_prime_UTR	30.0%
	ATAATAGACTTACGGTGATT+GGG	+	chr3_1:77170680-77170699	None:intergenic	30.0%
	TGTAATGATGAGTTACTGAT+TGG	-	chr3_1:77171046-77171065	Msa0376850:intron	30.0%
	TGTTAAATGAATGCTAGTTC+TGG	-	chr3_1:77171123-77171142	Msa0376850:intron	30.0%
	AGATGCAAGTTCTACAAAAT+AGG	+	chr3_1:77171274-77171293	None:intergenic	30.0%
!	ATTTTGTAGAACTTGCATCT+AGG	-	chr3_1:77171274-77171293	Msa0376850:intron	30.0%
	AAAAGATATAATTTCCTCGG+AGG	-	chr3_1:77171300-77171319	Msa0376850:intron	30.0%
	AAAGATATAATTTCCTCGGA+GGG	-	chr3_1:77171301-77171320	Msa0376850:intron	30.0%
	CAGCAAATGACAGTATTTAT+CGG	+	chr3_1:77171337-77171356	None:intergenic	30.0%
	TATTCAGAGTTGTCTTGAAA+AGG	+	chr3_1:77171491-77171510	None:intergenic	30.0%
	ATGTAAGCTTATGCATAAGT+GGG	+	chr3_1:77171581-77171600	None:intergenic	30.0%
	AATGTAAGCTTATGCATAAG+TGG	+	chr3_1:77171582-77171601	None:intergenic	30.0%
	TTTGTTTAGTGACACTGTTT+GGG	+	chr3_1:77171683-77171702	None:intergenic	30.0%
	TCACCCAAAAGTAAAAAATG+TGG	+	chr3_1:77171744-77171763	None:intergenic	30.0%
!	ATTCCATTTTACATCTGATG+CGG	-	chr3_1:77172164-77172183	Msa0376850:five_prime_UTR	30.0%
	GAAACCTAGGTTAATTGATT+GGG	+	chr3_1:77170502-77170521	None:intergenic	30.0%
	AGAAACCTAGGTTAATTGAT+TGG	+	chr3_1:77170503-77170522	None:intergenic	30.0%
	ACAATTCACAAAATGAGTTC+CGG	-	chr3_1:77170606-77170625	Msa0376850:three_prime_UTR	30.0%
	ATAATAGACTTACGGTGATT+GGG	+	chr3_1:77170680-77170699	None:intergenic	30.0%
	TGTAATGATGAGTTACTGAT+TGG	-	chr3_1:77171046-77171065	Msa0376850:intron	30.0%
	TGTTAAATGAATGCTAGTTC+TGG	-	chr3_1:77171123-77171142	Msa0376850:intron	30.0%
	AGATGCAAGTTCTACAAAAT+AGG	+	chr3_1:77171274-77171293	None:intergenic	30.0%
!	ATTTTGTAGAACTTGCATCT+AGG	-	chr3_1:77171274-77171293	Msa0376850:intron	30.0%
	AAAAGATATAATTTCCTCGG+AGG	-	chr3_1:77171300-77171319	Msa0376850:intron	30.0%
	AAAGATATAATTTCCTCGGA+GGG	-	chr3_1:77171301-77171320	Msa0376850:intron	30.0%
	CAGCAAATGACAGTATTTAT+CGG	+	chr3_1:77171337-77171356	None:intergenic	30.0%
	TATTCAGAGTTGTCTTGAAA+AGG	+	chr3_1:77171491-77171510	None:intergenic	30.0%
	ATGTAAGCTTATGCATAAGT+GGG	+	chr3_1:77171581-77171600	None:intergenic	30.0%
	AATGTAAGCTTATGCATAAG+TGG	+	chr3_1:77171582-77171601	None:intergenic	30.0%
	TTTGTTTAGTGACACTGTTT+GGG	+	chr3_1:77171683-77171702	None:intergenic	30.0%
	TCACCCAAAAGTAAAAAATG+TGG	+	chr3_1:77171744-77171763	None:intergenic	30.0%
!	ATTCCATTTTACATCTGATG+CGG	-	chr3_1:77172164-77172183	Msa0376850:five_prime_UTR	30.0%
	GATAATAGACTTACGGTGAT+TGG	+	chr3_1:77170681-77170700	None:intergenic	35.0%
	GAAGAGGGATAATAGACTTA+CGG	+	chr3_1:77170688-77170707	None:intergenic	35.0%
!	TCTTTTGTTACTGTTTGGGT+TGG	-	chr3_1:77170752-77170771	Msa0376850:CDS	35.0%
!!!	GTTTGGGTTGGTTTTTGTTT+AGG	-	chr3_1:77170764-77170783	Msa0376850:CDS	35.0%
!	ATTTTGTGCTGTGATCAAGT+TGG	-	chr3_1:77171241-77171260	Msa0376850:intron	35.0%
!	TTTTGTGCTGTGATCAAGTT+GGG	-	chr3_1:77171242-77171261	Msa0376850:intron	35.0%
	AAGATATAATTTCCTCGGAG+GGG	-	chr3_1:77171302-77171321	Msa0376850:intron	35.0%
	CTTCATTGGGATTTGAGAAT+TGG	-	chr3_1:77171460-77171479	Msa0376850:intron	35.0%
	GTTTGTTTAGTGACACTGTT+TGG	+	chr3_1:77171684-77171703	None:intergenic	35.0%
	TCATTCCATACTGTGTTTGT+AGG	-	chr3_1:77171897-77171916	Msa0376850:CDS	35.0%
!!	TAGTTTGTTGTAGCCAGATT+AGG	-	chr3_1:77171984-77172003	Msa0376850:intron	35.0%
!	TTCCATTTTACATCTGATGC+GGG	-	chr3_1:77172165-77172184	Msa0376850:five_prime_UTR	35.0%
	GATAATAGACTTACGGTGAT+TGG	+	chr3_1:77170681-77170700	None:intergenic	35.0%
	GAAGAGGGATAATAGACTTA+CGG	+	chr3_1:77170688-77170707	None:intergenic	35.0%
!	TCTTTTGTTACTGTTTGGGT+TGG	-	chr3_1:77170752-77170771	Msa0376850:CDS	35.0%
!!!	GTTTGGGTTGGTTTTTGTTT+AGG	-	chr3_1:77170764-77170783	Msa0376850:CDS	35.0%
!	ATTTTGTGCTGTGATCAAGT+TGG	-	chr3_1:77171241-77171260	Msa0376850:intron	35.0%
!	TTTTGTGCTGTGATCAAGTT+GGG	-	chr3_1:77171242-77171261	Msa0376850:intron	35.0%
	AAGATATAATTTCCTCGGAG+GGG	-	chr3_1:77171302-77171321	Msa0376850:intron	35.0%
	CTTCATTGGGATTTGAGAAT+TGG	-	chr3_1:77171460-77171479	Msa0376850:intron	35.0%
	GTTTGTTTAGTGACACTGTT+TGG	+	chr3_1:77171684-77171703	None:intergenic	35.0%
	TCATTCCATACTGTGTTTGT+AGG	-	chr3_1:77171897-77171916	Msa0376850:CDS	35.0%
!!	TAGTTTGTTGTAGCCAGATT+AGG	-	chr3_1:77171984-77172003	Msa0376850:intron	35.0%
!	TTCCATTTTACATCTGATGC+GGG	-	chr3_1:77172165-77172184	Msa0376850:five_prime_UTR	35.0%
!!!	AACTTCACATTTTGGTGCTC+TGG	-	chr3_1:77170548-77170567	Msa0376850:three_prime_UTR	40.0%
!	TTCGTAAACCCTAGTTTGTG+TGG	-	chr3_1:77170573-77170592	Msa0376850:three_prime_UTR	40.0%
	TTGTTCTGCCACACAAACTA+GGG	+	chr3_1:77170584-77170603	None:intergenic	40.0%
	ATTGTTCTGCCACACAAACT+AGG	+	chr3_1:77170585-77170604	None:intergenic	40.0%
	AAAATGAGTTCCGGCGAATT+GGG	-	chr3_1:77170615-77170634	Msa0376850:three_prime_UTR	40.0%
!!!	TGGGTTGGTTTTTGTTTAGG+CGG	-	chr3_1:77170767-77170786	Msa0376850:CDS	40.0%
	TTTGTTTAGGCGGAGAAGAT+CGG	-	chr3_1:77170777-77170796	Msa0376850:intron	40.0%
	GAAGATCGGTACTTTGTTGA+AGG	-	chr3_1:77170791-77170810	Msa0376850:intron	40.0%
!!	GATGATCTCGTTTTGAACTC+CGG	-	chr3_1:77170882-77170901	Msa0376850:intron	40.0%
!	CAATACCTTCTTTTCAACGG+CGG	+	chr3_1:77170982-77171001	None:intergenic	40.0%
!	TTTGTGCTGTGATCAAGTTG+GGG	-	chr3_1:77171243-77171262	Msa0376850:intron	40.0%
	ATCAACAAGACAATCTTCCC+AGG	+	chr3_1:77171648-77171667	None:intergenic	40.0%
	GATATAGGGCCAGTTATGAT+TGG	+	chr3_1:77171722-77171741	None:intergenic	40.0%
	TTCCATACTGTGTTTGTAGG+AGG	-	chr3_1:77171900-77171919	Msa0376850:CDS	40.0%
	TTCCTCCTACAAACACAGTA+TGG	+	chr3_1:77171905-77171924	None:intergenic	40.0%
	GAAGAAAGTGACGATGACAT+GGG	-	chr3_1:77171931-77171950	Msa0376850:intron	40.0%
	TTCCCGCATCAGATGTAAAA+TGG	+	chr3_1:77172170-77172189	None:intergenic	40.0%
	ATGCGGGAATATATCATACC+AGG	-	chr3_1:77172181-77172200	Msa0376850:five_prime_UTR	40.0%
!!!	AACTTCACATTTTGGTGCTC+TGG	-	chr3_1:77170548-77170567	Msa0376850:three_prime_UTR	40.0%
!	TTCGTAAACCCTAGTTTGTG+TGG	-	chr3_1:77170573-77170592	Msa0376850:three_prime_UTR	40.0%
	TTGTTCTGCCACACAAACTA+GGG	+	chr3_1:77170584-77170603	None:intergenic	40.0%
	ATTGTTCTGCCACACAAACT+AGG	+	chr3_1:77170585-77170604	None:intergenic	40.0%
	AAAATGAGTTCCGGCGAATT+GGG	-	chr3_1:77170615-77170634	Msa0376850:three_prime_UTR	40.0%
!!!	TGGGTTGGTTTTTGTTTAGG+CGG	-	chr3_1:77170767-77170786	Msa0376850:CDS	40.0%
	TTTGTTTAGGCGGAGAAGAT+CGG	-	chr3_1:77170777-77170796	Msa0376850:intron	40.0%
	GAAGATCGGTACTTTGTTGA+AGG	-	chr3_1:77170791-77170810	Msa0376850:intron	40.0%
!!	GATGATCTCGTTTTGAACTC+CGG	-	chr3_1:77170882-77170901	Msa0376850:intron	40.0%
!	CAATACCTTCTTTTCAACGG+CGG	+	chr3_1:77170982-77171001	None:intergenic	40.0%
!	TTTGTGCTGTGATCAAGTTG+GGG	-	chr3_1:77171243-77171262	Msa0376850:intron	40.0%
	ATCAACAAGACAATCTTCCC+AGG	+	chr3_1:77171648-77171667	None:intergenic	40.0%
	GATATAGGGCCAGTTATGAT+TGG	+	chr3_1:77171722-77171741	None:intergenic	40.0%
	TTCCATACTGTGTTTGTAGG+AGG	-	chr3_1:77171900-77171919	Msa0376850:CDS	40.0%
	TTCCTCCTACAAACACAGTA+TGG	+	chr3_1:77171905-77171924	None:intergenic	40.0%
	GAAGAAAGTGACGATGACAT+GGG	-	chr3_1:77171931-77171950	Msa0376850:intron	40.0%
	TTCCCGCATCAGATGTAAAA+TGG	+	chr3_1:77172170-77172189	None:intergenic	40.0%
	ATGCGGGAATATATCATACC+AGG	-	chr3_1:77172181-77172200	Msa0376850:five_prime_UTR	40.0%
	TGGCGTGTTATCAGAAACCT+AGG	+	chr3_1:77170515-77170534	None:intergenic	45.0%
	CAAAATGAGTTCCGGCGAAT+TGG	-	chr3_1:77170614-77170633	Msa0376850:three_prime_UTR	45.0%
	ACGTCACCGTTGAATCTTAC+TGG	-	chr3_1:77170820-77170839	Msa0376850:intron	45.0%
	GCTTGGCCAGTAAGATTCAA+CGG	+	chr3_1:77170829-77170848	None:intergenic	45.0%
	TCTGAGCAAGCTTAGCGAAT+AGG	+	chr3_1:77170851-77170870	None:intergenic	45.0%
!	GTCATCGTCACTTTCTTCCT+TGG	+	chr3_1:77171929-77171948	None:intergenic	45.0%
	GGAAGAAAGTGACGATGACA+TGG	-	chr3_1:77171930-77171949	Msa0376850:intron	45.0%
	CCTTGAAAGGAGTCCTAATC+TGG	+	chr3_1:77172000-77172019	None:intergenic	45.0%
	CCAGATTAGGACTCCTTTCA+AGG	-	chr3_1:77171997-77172016	Msa0376850:intron	45.0%
	TTAGGACTCCTTTCAAGGAG+TGG	-	chr3_1:77172002-77172021	Msa0376850:intron	45.0%
	TGGCGTGTTATCAGAAACCT+AGG	+	chr3_1:77170515-77170534	None:intergenic	45.0%
	CAAAATGAGTTCCGGCGAAT+TGG	-	chr3_1:77170614-77170633	Msa0376850:three_prime_UTR	45.0%
	ACGTCACCGTTGAATCTTAC+TGG	-	chr3_1:77170820-77170839	Msa0376850:intron	45.0%
	GCTTGGCCAGTAAGATTCAA+CGG	+	chr3_1:77170829-77170848	None:intergenic	45.0%
	TCTGAGCAAGCTTAGCGAAT+AGG	+	chr3_1:77170851-77170870	None:intergenic	45.0%
!	GTCATCGTCACTTTCTTCCT+TGG	+	chr3_1:77171929-77171948	None:intergenic	45.0%
	GGAAGAAAGTGACGATGACA+TGG	-	chr3_1:77171930-77171949	Msa0376850:intron	45.0%
	CCTTGAAAGGAGTCCTAATC+TGG	+	chr3_1:77172000-77172019	None:intergenic	45.0%
	CCAGATTAGGACTCCTTTCA+AGG	-	chr3_1:77171997-77172016	Msa0376850:intron	45.0%
	TTAGGACTCCTTTCAAGGAG+TGG	-	chr3_1:77172002-77172021	Msa0376850:intron	45.0%
	GTAAATGCAGCCCAATTCGC+CGG	+	chr3_1:77170628-77170647	None:intergenic	50.0%
	TGATTGGGATTCCATCGTCG+TGG	+	chr3_1:77170665-77170684	None:intergenic	50.0%
	GCAAGCTTAGCGAATAGGCT+TGG	+	chr3_1:77170846-77170865	None:intergenic	50.0%
	GTGTTTGTAGGAGGAAGCCA+AGG	-	chr3_1:77171909-77171928	Msa0376850:intron	50.0%
	GGATTCAGCCACTCCTTGAA+AGG	+	chr3_1:77172013-77172032	None:intergenic	50.0%
	GTAAATGCAGCCCAATTCGC+CGG	+	chr3_1:77170628-77170647	None:intergenic	50.0%
	TGATTGGGATTCCATCGTCG+TGG	+	chr3_1:77170665-77170684	None:intergenic	50.0%
	GCAAGCTTAGCGAATAGGCT+TGG	+	chr3_1:77170846-77170865	None:intergenic	50.0%
	GTGTTTGTAGGAGGAAGCCA+AGG	-	chr3_1:77171909-77171928	Msa0376850:intron	50.0%
	GGATTCAGCCACTCCTTGAA+AGG	+	chr3_1:77172013-77172032	None:intergenic	50.0%
	ATCGTCGTGGAGGATCAAGG+TGG	+	chr3_1:77170652-77170671	None:intergenic	55.0%
	ACCTTGATCCTCCACGACGA+TGG	-	chr3_1:77170651-77170670	Msa0376850:three_prime_UTR	55.0%
	TCCATCGTCGTGGAGGATCA+AGG	+	chr3_1:77170655-77170674	None:intergenic	55.0%
	TTGGGATTCCATCGTCGTGG+AGG	+	chr3_1:77170662-77170681	None:intergenic	55.0%
!!	GTTTTGAACTCCGGCGCTGT+TGG	-	chr3_1:77170891-77170910	Msa0376850:intron	55.0%
!	CTTTTCAACGGCGGCAGGTT+CGG	+	chr3_1:77170973-77170992	None:intergenic	55.0%
	ACCTGCCGCCGTTGAAAAGA+AGG	-	chr3_1:77170974-77170993	Msa0376850:intron	55.0%
!	ACCTTCTTTTCAACGGCGGC+AGG	+	chr3_1:77170978-77170997	None:intergenic	55.0%
	ATCGTCGTGGAGGATCAAGG+TGG	+	chr3_1:77170652-77170671	None:intergenic	55.0%
	ACCTTGATCCTCCACGACGA+TGG	-	chr3_1:77170651-77170670	Msa0376850:three_prime_UTR	55.0%
	TCCATCGTCGTGGAGGATCA+AGG	+	chr3_1:77170655-77170674	None:intergenic	55.0%
	TTGGGATTCCATCGTCGTGG+AGG	+	chr3_1:77170662-77170681	None:intergenic	55.0%
!!	GTTTTGAACTCCGGCGCTGT+TGG	-	chr3_1:77170891-77170910	Msa0376850:intron	55.0%
!	CTTTTCAACGGCGGCAGGTT+CGG	+	chr3_1:77170973-77170992	None:intergenic	55.0%
	ACCTGCCGCCGTTGAAAAGA+AGG	-	chr3_1:77170974-77170993	Msa0376850:intron	55.0%
!	ACCTTCTTTTCAACGGCGGC+AGG	+	chr3_1:77170978-77170997	None:intergenic	55.0%
	TTGAACTCCGGCGCTGTTGG+TGG	-	chr3_1:77170894-77170913	Msa0376850:intron	60.0%
	GGGTGCAGATACGGCAACAG+CGG	+	chr3_1:77170922-77170941	None:intergenic	60.0%
	ACCAGCAGCGGGTGCAGATA+CGG	+	chr3_1:77170931-77170950	None:intergenic	60.0%
	CGGATACAGAATCCCCTCCG+AGG	+	chr3_1:77171317-77171336	None:intergenic	60.0%
	TTGAACTCCGGCGCTGTTGG+TGG	-	chr3_1:77170894-77170913	Msa0376850:intron	60.0%
	GGGTGCAGATACGGCAACAG+CGG	+	chr3_1:77170922-77170941	None:intergenic	60.0%
	ACCAGCAGCGGGTGCAGATA+CGG	+	chr3_1:77170931-77170950	None:intergenic	60.0%
	CGGATACAGAATCCCCTCCG+AGG	+	chr3_1:77171317-77171336	None:intergenic	60.0%
	GTATCTGCACCCGCTGCTGG+TGG	-	chr3_1:77170930-77170949	Msa0376850:intron	65.0%
	GTATCTGCACCCGCTGCTGG+TGG	-	chr3_1:77170930-77170949	Msa0376850:intron	65.0%
	AGCGGCACCACCAACAGCGC+CGG	+	chr3_1:77170904-77170923	None:intergenic	70.0%
	GCCGTATCTGCACCCGCTGC+TGG	-	chr3_1:77170927-77170946	Msa0376850:intron	70.0%
	TCTGCACCCGCTGCTGGTGG+TGG	-	chr3_1:77170933-77170952	Msa0376850:intron	70.0%
	TGCGGCTCCACCACCAGCAG+CGG	+	chr3_1:77170943-77170962	None:intergenic	70.0%
	GGCAGGTTCGGCAGCTGCTG+CGG	+	chr3_1:77170961-77170980	None:intergenic	70.0%
	AGCGGCACCACCAACAGCGC+CGG	+	chr3_1:77170904-77170923	None:intergenic	70.0%
	GCCGTATCTGCACCCGCTGC+TGG	-	chr3_1:77170927-77170946	Msa0376850:intron	70.0%
	TCTGCACCCGCTGCTGGTGG+TGG	-	chr3_1:77170933-77170952	Msa0376850:intron	70.0%
	TGCGGCTCCACCACCAGCAG+CGG	+	chr3_1:77170943-77170962	None:intergenic	70.0%
	GGCAGGTTCGGCAGCTGCTG+CGG	+	chr3_1:77170961-77170980	None:intergenic	70.0%
	GCGGCTCCACCACCAGCAGC+GGG	+	chr3_1:77170942-77170961	None:intergenic	75.0%
	GCGGCTCCACCACCAGCAGC+GGG	+	chr3_1:77170942-77170961	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_1	gene	77170498	77172211	77170498	ID=Msa0376850;Name=Msa0376850
chr3_1	mRNA	77170498	77172211	77170498	ID=Msa0376850-mRNA-1;Parent=Msa0376850;Name=Msa0376850-mRNA-1;_AED=0.20;_eAED=0.20;_QI=120\|1\|1\|1\|1\|1\|3\|239\|110
chr3_1	exon	77170498	77170790	77170498	ID=Msa0376850-mRNA-1:exon:18419;Parent=Msa0376850-mRNA-1
chr3_1	exon	77171714	77171923	77171714	ID=Msa0376850-mRNA-1:exon:18418;Parent=Msa0376850-mRNA-1
chr3_1	exon	77172023	77172211	77172023	ID=Msa0376850-mRNA-1:exon:18417;Parent=Msa0376850-mRNA-1
chr3_1	five_prime_UTR	77172092	77172211	77172092	ID=Msa0376850-mRNA-1:five_prime_utr;Parent=Msa0376850-mRNA-1
chr3_1	CDS	77172023	77172091	77172023	ID=Msa0376850-mRNA-1:cds;Parent=Msa0376850-mRNA-1
chr3_1	CDS	77171714	77171923	77171714	ID=Msa0376850-mRNA-1:cds;Parent=Msa0376850-mRNA-1
chr3_1	CDS	77170737	77170790	77170737	ID=Msa0376850-mRNA-1:cds;Parent=Msa0376850-mRNA-1
chr3_1	three_prime_UTR	77170498	77170736	77170498	ID=Msa0376850-mRNA-1:three_prime_utr;Parent=Msa0376850-mRNA-1

Gene Sequence	>Msa0376850 ATGAGTTCCGGCGAATTGGGCTGCATTTACGCCACCTTGATCCTCCACGACGATGGAATCCCAATCACCGCGGAGAAGATCGGTACTTTGTTGAAGGCTGCTAACGTCACCGTTGAATCTTACTGGCCAAGCCTATTCGCTAAGCTTGCTCAGAGCAAGAACGTTGATGATCTCGTTTTGAACTCCGGCGCTGTTGGTGGTGCCGCTGTTGCCGTATCTGCACCCGCTGCTGGTGGTGGAGCCGCAGCAGCTGCCGAACCTGCCGCCGTTGAAAAGAAGGAGGAAGCCAAGGAAGAAAGTGACGATGACATGGGCTTTAGTTTGTTTGATTAA
Protein sequence	>Msa0376850 MSSGELGCIYATLILHDDGIPITAEKIGTLLKAANVTVESYWPSLFAKLAQSKNVDDLVLNSGAVGGAAVAVSAPAAGGGAAAAAEPAAVEKKEEAKEESDDDMGFSLFD*