AlfalfaGEDB | Msa0516830

M. sativa cultivar zhongmu-4 / Msa0516830

KEGG: K02942
GO: GO:0001932 GO:0001934 GO:0002181 GO:0003674 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005840 GO:0005844 GO:0005886 GO:0006412 GO:0006518 GO:0006807 GO:0008047 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009893 GO:0009987 GO:0010467 GO:0010562 GO:0010604 GO:0015934 GO:0016020 GO:0019207 GO:0019209 GO:0019220 GO:0019222 GO:0019538 GO:0019887 GO:0022625 GO:0022626 GO:0030234 GO:0030295 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032991 GO:0033674 GO:0034641 GO:0034645 GO:0042325 GO:0042327 GO:0042788 GO:0043043 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043549 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0044877 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071944 GO:0080090 GO:0098772 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00428.22

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0516830	XP_003602368.1	99.091	110	1	0	1	110	1	110	2.69e-70	215

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0516830	sp\|P52855\|RLA1_MAIZE	70.909	110	31	1	1	110	1	109	3.81e-29	104

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0516830	G7J8Y6	99.091	110	1	0	1	110	1	110	1.28e-70	215

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001760	Msa0516830	0.822050	2.914349e-53	-8.615850e-47
Msa0003030	Msa0516830	0.857543	1.519692e-62	-8.615850e-47
Msa0003330	Msa0516830	0.827845	1.249296e-54	-8.615850e-47
Msa0027590	Msa0516830	0.813632	2.320229e-51	-8.615850e-47
Msa0042510	Msa0516830	0.860072	2.665182e-63	-8.615850e-47
Msa0079560	Msa0516830	0.849110	3.991495e-60	-8.615850e-47
Msa0127230	Msa0516830	0.842143	3.094770e-58	-8.615850e-47
Msa0146910	Msa0516830	0.854251	1.394337e-61	-8.615850e-47
Msa0167860	Msa0516830	0.843740	1.163189e-58	-8.615850e-47
Msa0168320	Msa0516830	0.874305	7.493083e-68	-8.615850e-47
Msa0169710	Msa0516830	0.822900	1.849250e-53	-8.615850e-47
Msa0183840	Msa0516830	0.846014	2.833977e-59	-8.615850e-47
Msa0191550	Msa0516830	0.811180	7.965492e-51	-8.615850e-47
Msa0192300	Msa0516830	0.864549	1.122428e-64	-8.615850e-47
Msa0195030	Msa0516830	0.854353	1.302856e-61	-8.615850e-47
Msa0233550	Msa0516830	0.853826	1.848176e-61	-8.615850e-47
Msa0237180	Msa0516830	0.843899	1.054776e-58	-8.615850e-47
Msa0371490	Msa0516830	0.836807	7.535532e-57	-8.615850e-47
Msa0373350	Msa0516830	0.861438	1.025691e-63	-8.615850e-47
Msa0376850	Msa0516830	0.999783	0.000000e+00	-8.615850e-47
Msa0377370	Msa0516830	0.837106	6.322477e-57	-8.615850e-47
Msa0377520	Msa0516830	0.816021	6.853889e-52	-8.615850e-47
Msa0377610	Msa0516830	0.829942	3.881491e-55	-8.615850e-47
Msa0382670	Msa0516830	0.818231	2.183105e-52	-8.615850e-47
Msa0387290	Msa0516830	0.814127	1.804859e-51	-8.615850e-47
Msa0388520	Msa0516830	0.838977	2.086431e-57	-8.615850e-47
Msa0416470	Msa0516830	0.829109	6.185405e-55	-8.615850e-47
Msa0421920	Msa0516830	0.835157	1.975575e-56	-8.615850e-47
Msa0426940	Msa0516830	0.828495	8.711075e-55	-8.615850e-47
Msa0467740	Msa0516830	0.812843	3.456394e-51	-8.615850e-47
Msa0239420	Msa0516830	0.859938	2.924197e-63	-8.615850e-47
Msa0256350	Msa0516830	0.812013	5.247559e-51	-8.615850e-47
Msa0274940	Msa0516830	0.867316	1.496979e-65	-8.615850e-47
Msa0281330	Msa0516830	0.807622	4.620561e-50	-8.615850e-47
Msa0281370	Msa0516830	0.844039	9.675849e-59	-8.615850e-47
Msa0301250	Msa0516830	0.859321	4.483992e-63	-8.615850e-47
Msa0313060	Msa0516830	0.874529	6.289272e-68	-8.615850e-47
Msa0315430	Msa0516830	0.817680	2.908767e-52	-8.615850e-47
Msa0315660	Msa0516830	0.850963	1.208231e-60	-8.615850e-47
Msa0485220	Msa0516830	0.805982	1.026192e-49	-8.615850e-47
Msa0511760	Msa0516830	0.863991	1.675862e-64	-8.615850e-47
Msa0514130	Msa0516830	0.813204	2.881672e-51	-8.615850e-47
Msa0516830	Msa0547920	0.804120	2.515462e-49	-8.615850e-47
Msa0516830	Msa0573550	0.866163	3.486448e-65	-8.615850e-47
Msa0516830	Msa0574780	0.859183	4.934256e-63	-8.615850e-47
Msa0516830	Msa0578010	0.823715	1.193024e-53	-8.615850e-47
Msa0516830	Msa0597240	0.814597	1.421037e-51	-8.615850e-47
Msa0516830	Msa0602900	0.809983	1.444623e-50	-8.615850e-47
Msa0516830	Msa0605890	0.859438	4.135193e-63	-8.615850e-47
Msa0516830	Msa0609120	0.860223	2.399258e-63	-8.615850e-47
Msa0516830	Msa0619230	0.885709	6.433473e-72	-8.615850e-47
Msa0516830	Msa0621190	0.878578	2.506846e-69	-8.615850e-47
Msa0516830	Msa0643960	0.809945	1.471937e-50	-8.615850e-47
Msa0516830	Msa0656450	0.872796	2.414385e-67	-8.615850e-47
Msa0516830	Msa0686550	0.819728	9.974312e-53	-8.615850e-47
Msa0516830	Msa0691760	0.898903	3.359373e-77	-8.615850e-47
Msa0516830	Msa0694400	0.809843	1.548259e-50	-8.615850e-47
Msa0516830	Msa0700370	0.832108	1.140823e-55	-8.615850e-47
Msa0516830	Msa0704860	0.838320	3.084882e-57	-8.615850e-47
Msa0516830	Msa0834220	0.809904	1.502572e-50	-8.615850e-47
Msa0516830	Msa0902730	0.810627	1.049336e-50	-8.615850e-47
Msa0516830	Msa0929700	0.848957	4.400341e-60	-8.615850e-47
Msa0516830	Msa0930360	0.828561	8.393638e-55	-8.615850e-47
Msa0516830	Msa0930790	0.846067	2.741629e-59	-8.615850e-47
Msa0516830	Msa1078480	0.817206	3.718474e-52	-8.615850e-47
Msa0516830	Msa1078640	0.827467	1.539595e-54	-8.615850e-47
Msa0516830	Msa1082050	0.821457	3.997256e-53	-8.615850e-47
Msa0516830	Msa1126690	0.814996	1.159110e-51	-8.615850e-47
Msa0516830	Msa1165700	0.800403	1.465388e-48	-8.615850e-47
Msa0516830	Msa1169470	0.841941	3.501469e-58	-8.615850e-47
Msa0516830	Msa1227350	0.848961	4.389721e-60	-8.615850e-47
Msa0516830	Msa1235950	0.835684	1.454240e-56	-8.615850e-47
Msa0516830	Msa1235980	0.840093	1.070002e-57	-8.615850e-47
Msa0516830	Msa1237040	0.809801	1.581249e-50	-8.615850e-47
Msa0516830	Msa1284800	0.821409	4.100253e-53	-8.615850e-47
Msa0516830	Msa1317390	0.823788	1.146932e-53	-8.615850e-47
Msa0516830	Msa1351020	0.863261	2.824802e-64	-8.615850e-47
Msa0516830	Msa1387750	0.860355	2.188211e-63	-8.615850e-47
Msa0516830	Msa1390760	0.887262	1.664894e-72	-8.615850e-47
Msa0516830	Msa1407550	0.870815	1.097112e-66	-8.615850e-47
Msa0516830	Msa1413570	0.872804	2.400368e-67	-8.615850e-47
Msa0516830	Msa1432310	0.864227	1.415305e-64	-8.615850e-47
Msa0516830	Msa1446510	0.867135	1.710663e-65	-8.615850e-47
Msa0516830	Msa1465700	0.841850	3.700035e-58	-8.615850e-47
Msa0516830	Msa1465890	0.803447	3.471654e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0516830	MtrunA17_Chr3g0127831	99.091	110	1	0	1	110	1	110	2.47e-74	215
Msa0516830	MtrunA17_Chr2g0311301	98.182	110	2	0	1	110	1	110	8.45e-74	214
Msa0516830	MtrunA17_Chr4g0013621	91.818	110	9	0	1	110	1	110	2.10e-45	142
Msa0516830	MtrunA17_Chr8g0345951	64.545	110	37	2	1	110	1	108	1.12e-33	112
Msa0516830	MtrunA17_Chr7g0276131	62.712	59	22	0	1	59	1	59	7.89e-21	80.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0516830	AT5G24510.1	57.391	115	40	4	1	110	1	111	4.31e-36	119
Msa0516830	AT5G47700.1	57.658	111	45	1	2	110	3	113	8.30e-29	100
Msa0516830	AT5G47700.2	57.658	111	45	1	2	110	3	113	8.30e-29	100
Msa0516830	AT1G01100.4	60.185	108	42	1	4	110	5	112	4.43e-28	99.0
Msa0516830	AT1G01100.1	60.185	108	42	1	4	110	5	112	4.43e-28	99.0
Msa0516830	AT1G01100.2	60.185	108	42	1	4	110	5	112	4.43e-28	99.0
Msa0516830	AT1G01100.3	50.459	109	39	1	2	110	3	96	2.22e-27	96.7
Msa0516830	AT4G00810.1	54.955	111	48	1	2	110	3	113	2.98e-27	97.1
Msa0516830	AT4G00810.2	54.955	111	48	1	2	110	3	113	2.98e-27	97.1

Find 50 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATATCGAAACTTCACATTT+TGG	0.211931	3_4:-68381486	Msa0516830:five_prime_UTR
TTATAAAACAAAACAAGTTT+TGG	0.254235	3_4:+68379994	None:intergenic
TTCCCGCATCAGATGTAAAA+TGG	0.280734	3_4:+68379858	None:intergenic
CAAAATGAGTTCCGGCGAAT+TGG	0.294649	3_4:-68381412	Msa0516830:exon
GTCATCGTCACTTTCTTCCT+TGG	0.329724	3_4:+68380099	None:intergenic
GATAATAGACTTACGGTGAT+TGG	0.337914	3_4:+68381348	None:intergenic
AGAAACCTAGGTTAATTGAT+TGG	0.340250	3_4:+68381526	None:intergenic
ACGTCACCGTTGAATCTTAC+TGG	0.348075	3_4:-68381206	Msa0516830:CDS
AAAATGAGTTCCGGCGAATT+GGG	0.368280	3_4:-68381411	Msa0516830:CDS
TAGTTTGTTGTAGCCAGATT+AGG	0.371827	3_4:-68380041	Msa0516830:three_prime_UTR
CCTTGAAAGGAGTCCTAATC+TGG	0.373967	3_4:+68380028	None:intergenic
CCAGATTAGGACTCCTTTCA+AGG	0.391998	3_4:-68380028	Msa0516830:three_prime_UTR
TCTGAGCAAGCTTAGCGAAT+AGG	0.392998	3_4:+68381178	None:intergenic
GAAACCTAGGTTAATTGATT+GGG	0.394188	3_4:+68381527	None:intergenic
GCCGTATCTGCACCCGCTGC+TGG	0.401410	3_4:-68381099	Msa0516830:CDS
TCTGCACCCGCTGCTGGTGG+TGG	0.439771	3_4:-68381093	Msa0516830:CDS
ATAATAGACTTACGGTGATT+GGG	0.445449	3_4:+68381349	None:intergenic
ATTGTTCTGCCACACAAACT+AGG	0.448236	3_4:+68381444	None:intergenic
GCGGCTCCACCACCAGCAGC+GGG	0.451994	3_4:+68381087	None:intergenic
GAAGATCGGTACTTTGTTGA+AGG	0.461021	3_4:-68381235	Msa0516830:CDS
ACCTGCCGCCGTTGAAAAGA+AGG	0.476631	3_4:-68381052	Msa0516830:intron
TGCGGCTCCACCACCAGCAG+CGG	0.489051	3_4:+68381086	None:intergenic
TTGAACTCCGGCGCTGTTGG+TGG	0.510539	3_4:-68381132	Msa0516830:CDS
TGAAGTTTCGATATATATAG+TGG	0.520113	3_4:+68381494	None:intergenic
ACAATTCACAAAATGAGTTC+CGG	0.522809	3_4:-68381420	Msa0516830:exon
GGAAGAAAGTGACGATGACA+TGG	0.523361	3_4:-68380095	Msa0516830:CDS
AGCGGCACCACCAACAGCGC+CGG	0.527844	3_4:+68381125	None:intergenic
ACCAGCAGCGGGTGCAGATA+CGG	0.530473	3_4:+68381098	None:intergenic
GGATTCAGCCACTCCTTGAA+AGG	0.532068	3_4:+68380015	None:intergenic
TCCATCGTCGTGGAGGATCA+AGG	0.532980	3_4:+68381374	None:intergenic
GCAAGCTTAGCGAATAGGCT+TGG	0.542190	3_4:+68381183	None:intergenic
TTTGTTTAGGCGGAGAAGAT+CGG	0.543289	3_4:-68381249	Msa0516830:intron
GGCAGGTTCGGCAGCTGCTG+CGG	0.548908	3_4:+68381068	None:intergenic
TTGTTCTGCCACACAAACTA+GGG	0.550348	3_4:+68381445	None:intergenic
GTAAATGCAGCCCAATTCGC+CGG	0.571217	3_4:+68381401	None:intergenic
ACCTTGATCCTCCACGACGA+TGG	0.573503	3_4:-68381375	Msa0516830:CDS
AAGGCCCAATCAATTAACCT+AGG	0.578918	3_4:-68381531	Msa0516830:five_prime_UTR
TTAGGACTCCTTTCAAGGAG+TGG	0.585485	3_4:-68380023	Msa0516830:three_prime_UTR
TTCGTAAACCCTAGTTTGTG+TGG	0.589308	3_4:-68381453	Msa0516830:five_prime_UTR
AAAATCTTTCAAAATTAACC+TGG	0.590654	3_4:+68379826	None:intergenic
GTATCTGCACCCGCTGCTGG+TGG	0.603091	3_4:-68381096	Msa0516830:CDS
ATGCGGGAATATATCATACC+AGG	0.604298	3_4:-68379844	Msa0516830:three_prime_UTR
TGGCGTGTTATCAGAAACCT+AGG	0.605084	3_4:+68381514	None:intergenic
GCTTGGCCAGTAAGATTCAA+CGG	0.629260	3_4:+68381200	None:intergenic
TTGGGATTCCATCGTCGTGG+AGG	0.650788	3_4:+68381367	None:intergenic
GTGTTTGTAGGAGGAAGCCA+AGG	0.654949	3_4:-68380116	Msa0516830:intron
TGATTGGGATTCCATCGTCG+TGG	0.662981	3_4:+68381364	None:intergenic
GGGTGCAGATACGGCAACAG+CGG	0.665524	3_4:+68381107	None:intergenic
GAAGAAAGTGACGATGACAT+GGG	0.678372	3_4:-68380094	Msa0516830:CDS
ATCGTCGTGGAGGATCAAGG+TGG	0.743990	3_4:+68381377	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATTATATGTTTTTATCTTA+AGG	+	chr3_4:68380513-68380532	None:intergenic	10.0%
!!!	AATTATATGTTTTTATCTTA+AGG	+	chr3_4:68380513-68380532	None:intergenic	10.0%
!!	AATTGTATTGAAATTAGAAA+CGG	-	chr3_4:68380414-68380433	Msa0516830:intron	15.0%
!!	TATAAAAGATATAATTTCCT+CGG	-	chr3_4:68380636-68380655	Msa0516830:intron	15.0%
!!!	TTTTCATGCAAAATAATTTT+TGG	+	chr3_4:68380707-68380726	None:intergenic	15.0%
!!!	TTATAAAACAAAACAAGTTT+TGG	+	chr3_4:68381373-68381392	None:intergenic	15.0%
!!!	CTCATTTTATTGTTAAATTT+TGG	-	chr3_4:68381428-68381447	Msa0516830:exon	15.0%
!!	AATTGTATTGAAATTAGAAA+CGG	-	chr3_4:68380414-68380433	Msa0516830:intron	15.0%
!!	TATAAAAGATATAATTTCCT+CGG	-	chr3_4:68380636-68380655	Msa0516830:intron	15.0%
!!!	TTTTCATGCAAAATAATTTT+TGG	+	chr3_4:68380707-68380726	None:intergenic	15.0%
!!!	TTATAAAACAAAACAAGTTT+TGG	+	chr3_4:68381373-68381392	None:intergenic	15.0%
!!!	CTCATTTTATTGTTAAATTT+TGG	-	chr3_4:68381428-68381447	Msa0516830:exon	15.0%
!!!	ATTTTTCTTTTGTTACTGTT+TGG	-	chr3_4:68380085-68380104	Msa0516830:CDS	20.0%
!!!	TTTTTCTTTTGTTACTGTTT+GGG	-	chr3_4:68380086-68380105	Msa0516830:CDS	20.0%
!!!	ATGAAAACATGTAGTTATTT+TGG	-	chr3_4:68380744-68380763	Msa0516830:intron	20.0%
!!!	TAATTGTTGTTTTTCTTCAT+TGG	-	chr3_4:68380785-68380804	Msa0516830:intron	20.0%
!!!	AATTGTTGTTTTTCTTCATT+GGG	-	chr3_4:68380786-68380805	Msa0516830:intron	20.0%
!!	AAGTAAAAAATGTGGATATA+GGG	+	chr3_4:68381075-68381094	None:intergenic	20.0%
!!	AAAGTAAAAAATGTGGATAT+AGG	+	chr3_4:68381076-68381095	None:intergenic	20.0%
!!!	ATATCCACATTTTTTACTTT+TGG	-	chr3_4:68381076-68381095	Msa0516830:CDS	20.0%
!!!	TATCCACATTTTTTACTTTT+GGG	-	chr3_4:68381077-68381096	Msa0516830:CDS	20.0%
!!!	ATTTTTCTTTTGTTACTGTT+TGG	-	chr3_4:68380085-68380104	Msa0516830:CDS	20.0%
!!!	TTTTTCTTTTGTTACTGTTT+GGG	-	chr3_4:68380086-68380105	Msa0516830:CDS	20.0%
!!!	ATGAAAACATGTAGTTATTT+TGG	-	chr3_4:68380744-68380763	Msa0516830:intron	20.0%
!!!	TAATTGTTGTTTTTCTTCAT+TGG	-	chr3_4:68380785-68380804	Msa0516830:intron	20.0%
!!!	AATTGTTGTTTTTCTTCATT+GGG	-	chr3_4:68380786-68380805	Msa0516830:intron	20.0%
!!	AAGTAAAAAATGTGGATATA+GGG	+	chr3_4:68381075-68381094	None:intergenic	20.0%
!!	AAAGTAAAAAATGTGGATAT+AGG	+	chr3_4:68381076-68381095	None:intergenic	20.0%
!!!	ATATCCACATTTTTTACTTT+TGG	-	chr3_4:68381076-68381095	Msa0516830:CDS	20.0%
!!!	TATCCACATTTTTTACTTTT+GGG	-	chr3_4:68381077-68381096	Msa0516830:CDS	20.0%
!	TGAAGTTTCGATATATATAG+TGG	+	chr3_4:68379873-68379892	None:intergenic	25.0%
!!	TATATCGAAACTTCACATTT+TGG	-	chr3_4:68379878-68379897	Msa0516830:three_prime_UTR	25.0%
!!	AAACAATACCTTCTTTTCAA+CGG	+	chr3_4:68380323-68380342	None:intergenic	25.0%
!	GACAACTCTGAATATAATTA+TGG	-	chr3_4:68380836-68380855	Msa0516830:intron	25.0%
!!!	ATAAAGTTAACTTGTTTTCC+TGG	-	chr3_4:68380966-68380985	Msa0516830:intron	25.0%
!!!	TAAAGTTAACTTGTTTTCCT+GGG	-	chr3_4:68380967-68380986	Msa0516830:intron	25.0%
!	TTAAATAAGCCAATCATAAC+TGG	-	chr3_4:68381049-68381068	Msa0516830:intron	25.0%
!	TGAAGTTTCGATATATATAG+TGG	+	chr3_4:68379873-68379892	None:intergenic	25.0%
!!	TATATCGAAACTTCACATTT+TGG	-	chr3_4:68379878-68379897	Msa0516830:three_prime_UTR	25.0%
!!	AAACAATACCTTCTTTTCAA+CGG	+	chr3_4:68380323-68380342	None:intergenic	25.0%
!	GACAACTCTGAATATAATTA+TGG	-	chr3_4:68380836-68380855	Msa0516830:intron	25.0%
!!!	ATAAAGTTAACTTGTTTTCC+TGG	-	chr3_4:68380966-68380985	Msa0516830:intron	25.0%
!!!	TAAAGTTAACTTGTTTTCCT+GGG	-	chr3_4:68380967-68380986	Msa0516830:intron	25.0%
!	TTAAATAAGCCAATCATAAC+TGG	-	chr3_4:68381049-68381068	Msa0516830:intron	25.0%
	GAAACCTAGGTTAATTGATT+GGG	+	chr3_4:68379840-68379859	None:intergenic	30.0%
	AGAAACCTAGGTTAATTGAT+TGG	+	chr3_4:68379841-68379860	None:intergenic	30.0%
	ACAATTCACAAAATGAGTTC+CGG	-	chr3_4:68379944-68379963	Msa0516830:three_prime_UTR	30.0%
	ATAATAGACTTACGGTGATT+GGG	+	chr3_4:68380018-68380037	None:intergenic	30.0%
	GAAGAGAGATAATAGACTTA+CGG	+	chr3_4:68380026-68380045	None:intergenic	30.0%
!	GTAATGATGAGTTACTGATT+TGG	-	chr3_4:68380385-68380404	Msa0516830:intron	30.0%
	TGTTAAATGAATGCTAGTTC+TGG	-	chr3_4:68380462-68380481	Msa0516830:intron	30.0%
	AGATGCAAGTTCTACAAAAT+AGG	+	chr3_4:68380613-68380632	None:intergenic	30.0%
!	ATTTTGTAGAACTTGCATCT+AGG	-	chr3_4:68380613-68380632	Msa0516830:intron	30.0%
	AAAAGATATAATTTCCTCGG+AGG	-	chr3_4:68380639-68380658	Msa0516830:intron	30.0%
	AAAGATATAATTTCCTCGGA+GGG	-	chr3_4:68380640-68380659	Msa0516830:intron	30.0%
	CAGCAAATGACAGTATTTAT+CGG	+	chr3_4:68380676-68380695	None:intergenic	30.0%
	TATTCAGAGTTGTCTTGAAA+AGG	+	chr3_4:68380830-68380849	None:intergenic	30.0%
	ATGTAAGCTTATGCATAAGT+GGG	+	chr3_4:68380920-68380939	None:intergenic	30.0%
	AATGTAAGCTTATGCATAAG+TGG	+	chr3_4:68380921-68380940	None:intergenic	30.0%
	TTTGTTTAGTGACACTGTTT+GGG	+	chr3_4:68381022-68381041	None:intergenic	30.0%
	TCACCCAAAAGTAAAAAATG+TGG	+	chr3_4:68381083-68381102	None:intergenic	30.0%
!	ATTCCATTTTACATCTGATG+CGG	-	chr3_4:68381503-68381522	Msa0516830:five_prime_UTR	30.0%
	GAAACCTAGGTTAATTGATT+GGG	+	chr3_4:68379840-68379859	None:intergenic	30.0%
	AGAAACCTAGGTTAATTGAT+TGG	+	chr3_4:68379841-68379860	None:intergenic	30.0%
	ACAATTCACAAAATGAGTTC+CGG	-	chr3_4:68379944-68379963	Msa0516830:three_prime_UTR	30.0%
	ATAATAGACTTACGGTGATT+GGG	+	chr3_4:68380018-68380037	None:intergenic	30.0%
	GAAGAGAGATAATAGACTTA+CGG	+	chr3_4:68380026-68380045	None:intergenic	30.0%
!	GTAATGATGAGTTACTGATT+TGG	-	chr3_4:68380385-68380404	Msa0516830:intron	30.0%
	TGTTAAATGAATGCTAGTTC+TGG	-	chr3_4:68380462-68380481	Msa0516830:intron	30.0%
	AGATGCAAGTTCTACAAAAT+AGG	+	chr3_4:68380613-68380632	None:intergenic	30.0%
!	ATTTTGTAGAACTTGCATCT+AGG	-	chr3_4:68380613-68380632	Msa0516830:intron	30.0%
	AAAAGATATAATTTCCTCGG+AGG	-	chr3_4:68380639-68380658	Msa0516830:intron	30.0%
	AAAGATATAATTTCCTCGGA+GGG	-	chr3_4:68380640-68380659	Msa0516830:intron	30.0%
	CAGCAAATGACAGTATTTAT+CGG	+	chr3_4:68380676-68380695	None:intergenic	30.0%
	TATTCAGAGTTGTCTTGAAA+AGG	+	chr3_4:68380830-68380849	None:intergenic	30.0%
	ATGTAAGCTTATGCATAAGT+GGG	+	chr3_4:68380920-68380939	None:intergenic	30.0%
	AATGTAAGCTTATGCATAAG+TGG	+	chr3_4:68380921-68380940	None:intergenic	30.0%
	TTTGTTTAGTGACACTGTTT+GGG	+	chr3_4:68381022-68381041	None:intergenic	30.0%
	TCACCCAAAAGTAAAAAATG+TGG	+	chr3_4:68381083-68381102	None:intergenic	30.0%
!	ATTCCATTTTACATCTGATG+CGG	-	chr3_4:68381503-68381522	Msa0516830:five_prime_UTR	30.0%
	GATAATAGACTTACGGTGAT+TGG	+	chr3_4:68380019-68380038	None:intergenic	35.0%
	CGATTGGGAGATTATAGAAA+TGG	+	chr3_4:68380053-68380072	None:intergenic	35.0%
	AATGAGATCTATGAGCGATT+GGG	+	chr3_4:68380068-68380087	None:intergenic	35.0%
	AAATGAGATCTATGAGCGAT+TGG	+	chr3_4:68380069-68380088	None:intergenic	35.0%
!	TCTTTTGTTACTGTTTGGGT+TGG	-	chr3_4:68380090-68380109	Msa0516830:CDS	35.0%
!!!	GTTTGGGTTGGTTTTTGTTT+AGG	-	chr3_4:68380102-68380121	Msa0516830:CDS	35.0%
!	ATTTTGTGCTGTGATCAAGT+TGG	-	chr3_4:68380580-68380599	Msa0516830:intron	35.0%
!	TTTTGTGCTGTGATCAAGTT+GGG	-	chr3_4:68380581-68380600	Msa0516830:intron	35.0%
	AAGATATAATTTCCTCGGAG+GGG	-	chr3_4:68380641-68380660	Msa0516830:intron	35.0%
	CTTCATTGGGATTTGAGAAT+TGG	-	chr3_4:68380799-68380818	Msa0516830:intron	35.0%
	GTTTGTTTAGTGACACTGTT+TGG	+	chr3_4:68381023-68381042	None:intergenic	35.0%
	TCATTCCATACTGTGTTTGT+AGG	-	chr3_4:68381236-68381255	Msa0516830:CDS	35.0%
!!	TAGTTTGTTGTAGCCAGATT+AGG	-	chr3_4:68381323-68381342	Msa0516830:intron	35.0%
!	TTCCATTTTACATCTGATGC+GGG	-	chr3_4:68381504-68381523	Msa0516830:five_prime_UTR	35.0%
	GATAATAGACTTACGGTGAT+TGG	+	chr3_4:68380019-68380038	None:intergenic	35.0%
	CGATTGGGAGATTATAGAAA+TGG	+	chr3_4:68380053-68380072	None:intergenic	35.0%
	AATGAGATCTATGAGCGATT+GGG	+	chr3_4:68380068-68380087	None:intergenic	35.0%
	AAATGAGATCTATGAGCGAT+TGG	+	chr3_4:68380069-68380088	None:intergenic	35.0%
!	TCTTTTGTTACTGTTTGGGT+TGG	-	chr3_4:68380090-68380109	Msa0516830:CDS	35.0%
!!!	GTTTGGGTTGGTTTTTGTTT+AGG	-	chr3_4:68380102-68380121	Msa0516830:CDS	35.0%
!	ATTTTGTGCTGTGATCAAGT+TGG	-	chr3_4:68380580-68380599	Msa0516830:intron	35.0%
!	TTTTGTGCTGTGATCAAGTT+GGG	-	chr3_4:68380581-68380600	Msa0516830:intron	35.0%
	AAGATATAATTTCCTCGGAG+GGG	-	chr3_4:68380641-68380660	Msa0516830:intron	35.0%
	CTTCATTGGGATTTGAGAAT+TGG	-	chr3_4:68380799-68380818	Msa0516830:intron	35.0%
	GTTTGTTTAGTGACACTGTT+TGG	+	chr3_4:68381023-68381042	None:intergenic	35.0%
	TCATTCCATACTGTGTTTGT+AGG	-	chr3_4:68381236-68381255	Msa0516830:CDS	35.0%
!!	TAGTTTGTTGTAGCCAGATT+AGG	-	chr3_4:68381323-68381342	Msa0516830:intron	35.0%
!	TTCCATTTTACATCTGATGC+GGG	-	chr3_4:68381504-68381523	Msa0516830:five_prime_UTR	35.0%
!!!	AACTTCACATTTTGGTGCTC+TGG	-	chr3_4:68379886-68379905	Msa0516830:three_prime_UTR	40.0%
!	TTCGTAAACCCTAGTTTGTG+TGG	-	chr3_4:68379911-68379930	Msa0516830:three_prime_UTR	40.0%
	TTGTTCTGCCACACAAACTA+GGG	+	chr3_4:68379922-68379941	None:intergenic	40.0%
	ATTGTTCTGCCACACAAACT+AGG	+	chr3_4:68379923-68379942	None:intergenic	40.0%
	AAAATGAGTTCCGGCGAATT+GGG	-	chr3_4:68379953-68379972	Msa0516830:three_prime_UTR	40.0%
!!!	TGGGTTGGTTTTTGTTTAGG+CGG	-	chr3_4:68380105-68380124	Msa0516830:CDS	40.0%
	TTTGTTTAGGCGGAGAAGAT+CGG	-	chr3_4:68380115-68380134	Msa0516830:intron	40.0%
	GAAGATCGGTACTTTGTTGA+AGG	-	chr3_4:68380129-68380148	Msa0516830:intron	40.0%
!!	GATGATCTCGTTTTGAACTC+CGG	-	chr3_4:68380220-68380239	Msa0516830:intron	40.0%
!	CAATACCTTCTTTTCAACGG+CGG	+	chr3_4:68380320-68380339	None:intergenic	40.0%
!	TTTGTGCTGTGATCAAGTTG+GGG	-	chr3_4:68380582-68380601	Msa0516830:intron	40.0%
	ATCAACAAGACAATCTTCCC+AGG	+	chr3_4:68380987-68381006	None:intergenic	40.0%
	GATATAGGGCCAGTTATGAT+TGG	+	chr3_4:68381061-68381080	None:intergenic	40.0%
	TTCCATACTGTGTTTGTAGG+AGG	-	chr3_4:68381239-68381258	Msa0516830:CDS	40.0%
	TTCCTCCTACAAACACAGTA+TGG	+	chr3_4:68381244-68381263	None:intergenic	40.0%
	GAAGAAAGTGACGATGACAT+GGG	-	chr3_4:68381270-68381289	Msa0516830:intron	40.0%
	TTCCCGCATCAGATGTAAAA+TGG	+	chr3_4:68381509-68381528	None:intergenic	40.0%
	ATGCGGGAATATATCATACC+AGG	-	chr3_4:68381520-68381539	Msa0516830:five_prime_UTR	40.0%
!!!	AACTTCACATTTTGGTGCTC+TGG	-	chr3_4:68379886-68379905	Msa0516830:three_prime_UTR	40.0%
!	TTCGTAAACCCTAGTTTGTG+TGG	-	chr3_4:68379911-68379930	Msa0516830:three_prime_UTR	40.0%
	TTGTTCTGCCACACAAACTA+GGG	+	chr3_4:68379922-68379941	None:intergenic	40.0%
	ATTGTTCTGCCACACAAACT+AGG	+	chr3_4:68379923-68379942	None:intergenic	40.0%
	AAAATGAGTTCCGGCGAATT+GGG	-	chr3_4:68379953-68379972	Msa0516830:three_prime_UTR	40.0%
!!!	TGGGTTGGTTTTTGTTTAGG+CGG	-	chr3_4:68380105-68380124	Msa0516830:CDS	40.0%
	TTTGTTTAGGCGGAGAAGAT+CGG	-	chr3_4:68380115-68380134	Msa0516830:intron	40.0%
	GAAGATCGGTACTTTGTTGA+AGG	-	chr3_4:68380129-68380148	Msa0516830:intron	40.0%
!!	GATGATCTCGTTTTGAACTC+CGG	-	chr3_4:68380220-68380239	Msa0516830:intron	40.0%
!	CAATACCTTCTTTTCAACGG+CGG	+	chr3_4:68380320-68380339	None:intergenic	40.0%
!	TTTGTGCTGTGATCAAGTTG+GGG	-	chr3_4:68380582-68380601	Msa0516830:intron	40.0%
	ATCAACAAGACAATCTTCCC+AGG	+	chr3_4:68380987-68381006	None:intergenic	40.0%
	GATATAGGGCCAGTTATGAT+TGG	+	chr3_4:68381061-68381080	None:intergenic	40.0%
	TTCCATACTGTGTTTGTAGG+AGG	-	chr3_4:68381239-68381258	Msa0516830:CDS	40.0%
	TTCCTCCTACAAACACAGTA+TGG	+	chr3_4:68381244-68381263	None:intergenic	40.0%
	GAAGAAAGTGACGATGACAT+GGG	-	chr3_4:68381270-68381289	Msa0516830:intron	40.0%
	TTCCCGCATCAGATGTAAAA+TGG	+	chr3_4:68381509-68381528	None:intergenic	40.0%
	ATGCGGGAATATATCATACC+AGG	-	chr3_4:68381520-68381539	Msa0516830:five_prime_UTR	40.0%
	TGGCGTGTTATCAGAAACCT+AGG	+	chr3_4:68379853-68379872	None:intergenic	45.0%
	CAAAATGAGTTCCGGCGAAT+TGG	-	chr3_4:68379952-68379971	Msa0516830:three_prime_UTR	45.0%
	ACGTCACCGTTGAATCTTAC+TGG	-	chr3_4:68380158-68380177	Msa0516830:intron	45.0%
	GCTTGGCCAGTAAGATTCAA+CGG	+	chr3_4:68380167-68380186	None:intergenic	45.0%
	TCTGAGCAAGCTTAGCGAAT+AGG	+	chr3_4:68380189-68380208	None:intergenic	45.0%
!	GTCATCGTCACTTTCTTCCT+TGG	+	chr3_4:68381268-68381287	None:intergenic	45.0%
	GGAAGAAAGTGACGATGACA+TGG	-	chr3_4:68381269-68381288	Msa0516830:intron	45.0%
	CCTTGAAAGGAGTCCTAATC+TGG	+	chr3_4:68381339-68381358	None:intergenic	45.0%
	CCAGATTAGGACTCCTTTCA+AGG	-	chr3_4:68381336-68381355	Msa0516830:intron	45.0%
	TTAGGACTCCTTTCAAGGAG+TGG	-	chr3_4:68381341-68381360	Msa0516830:intron	45.0%
	TGGCGTGTTATCAGAAACCT+AGG	+	chr3_4:68379853-68379872	None:intergenic	45.0%
	CAAAATGAGTTCCGGCGAAT+TGG	-	chr3_4:68379952-68379971	Msa0516830:three_prime_UTR	45.0%
	ACGTCACCGTTGAATCTTAC+TGG	-	chr3_4:68380158-68380177	Msa0516830:intron	45.0%
	GCTTGGCCAGTAAGATTCAA+CGG	+	chr3_4:68380167-68380186	None:intergenic	45.0%
	TCTGAGCAAGCTTAGCGAAT+AGG	+	chr3_4:68380189-68380208	None:intergenic	45.0%
!	GTCATCGTCACTTTCTTCCT+TGG	+	chr3_4:68381268-68381287	None:intergenic	45.0%
	GGAAGAAAGTGACGATGACA+TGG	-	chr3_4:68381269-68381288	Msa0516830:intron	45.0%
	CCTTGAAAGGAGTCCTAATC+TGG	+	chr3_4:68381339-68381358	None:intergenic	45.0%
	CCAGATTAGGACTCCTTTCA+AGG	-	chr3_4:68381336-68381355	Msa0516830:intron	45.0%
	TTAGGACTCCTTTCAAGGAG+TGG	-	chr3_4:68381341-68381360	Msa0516830:intron	45.0%
	GTAAATGCAGCCCAATTCGC+CGG	+	chr3_4:68379966-68379985	None:intergenic	50.0%
	TGATTGGGATTCCATCGTCG+TGG	+	chr3_4:68380003-68380022	None:intergenic	50.0%
	GCAAGCTTAGCGAATAGGCT+TGG	+	chr3_4:68380184-68380203	None:intergenic	50.0%
	GTGTTTGTAGGAGGAAGCCA+AGG	-	chr3_4:68381248-68381267	Msa0516830:intron	50.0%
	GGATTCAGCCACTCCTTGAA+AGG	+	chr3_4:68381352-68381371	None:intergenic	50.0%
	GTAAATGCAGCCCAATTCGC+CGG	+	chr3_4:68379966-68379985	None:intergenic	50.0%
	TGATTGGGATTCCATCGTCG+TGG	+	chr3_4:68380003-68380022	None:intergenic	50.0%
	GCAAGCTTAGCGAATAGGCT+TGG	+	chr3_4:68380184-68380203	None:intergenic	50.0%
	GTGTTTGTAGGAGGAAGCCA+AGG	-	chr3_4:68381248-68381267	Msa0516830:intron	50.0%
	GGATTCAGCCACTCCTTGAA+AGG	+	chr3_4:68381352-68381371	None:intergenic	50.0%
	ATCGTCGTGGAGGATCAAGG+TGG	+	chr3_4:68379990-68380009	None:intergenic	55.0%
	ACCTTGATCCTCCACGACGA+TGG	-	chr3_4:68379989-68380008	Msa0516830:three_prime_UTR	55.0%
	TCCATCGTCGTGGAGGATCA+AGG	+	chr3_4:68379993-68380012	None:intergenic	55.0%
	TTGGGATTCCATCGTCGTGG+AGG	+	chr3_4:68380000-68380019	None:intergenic	55.0%
!!	GTTTTGAACTCCGGCGCTGT+TGG	-	chr3_4:68380229-68380248	Msa0516830:intron	55.0%
!	CTTTTCAACGGCGGCAGGTT+CGG	+	chr3_4:68380311-68380330	None:intergenic	55.0%
	ACCTGCCGCCGTTGAAAAGA+AGG	-	chr3_4:68380312-68380331	Msa0516830:intron	55.0%
!	ACCTTCTTTTCAACGGCGGC+AGG	+	chr3_4:68380316-68380335	None:intergenic	55.0%
	ATCGTCGTGGAGGATCAAGG+TGG	+	chr3_4:68379990-68380009	None:intergenic	55.0%
	ACCTTGATCCTCCACGACGA+TGG	-	chr3_4:68379989-68380008	Msa0516830:three_prime_UTR	55.0%
	TCCATCGTCGTGGAGGATCA+AGG	+	chr3_4:68379993-68380012	None:intergenic	55.0%
	TTGGGATTCCATCGTCGTGG+AGG	+	chr3_4:68380000-68380019	None:intergenic	55.0%
!!	GTTTTGAACTCCGGCGCTGT+TGG	-	chr3_4:68380229-68380248	Msa0516830:intron	55.0%
!	CTTTTCAACGGCGGCAGGTT+CGG	+	chr3_4:68380311-68380330	None:intergenic	55.0%
	ACCTGCCGCCGTTGAAAAGA+AGG	-	chr3_4:68380312-68380331	Msa0516830:intron	55.0%
!	ACCTTCTTTTCAACGGCGGC+AGG	+	chr3_4:68380316-68380335	None:intergenic	55.0%
	TTGAACTCCGGCGCTGTTGG+TGG	-	chr3_4:68380232-68380251	Msa0516830:intron	60.0%
	GGGTGCAGATACGGCAACAG+CGG	+	chr3_4:68380260-68380279	None:intergenic	60.0%
	ACCAGCAGCGGGTGCAGATA+CGG	+	chr3_4:68380269-68380288	None:intergenic	60.0%
	CGGATACAGAATCCCCTCCG+AGG	+	chr3_4:68380656-68380675	None:intergenic	60.0%
	TTGAACTCCGGCGCTGTTGG+TGG	-	chr3_4:68380232-68380251	Msa0516830:intron	60.0%
	GGGTGCAGATACGGCAACAG+CGG	+	chr3_4:68380260-68380279	None:intergenic	60.0%
	ACCAGCAGCGGGTGCAGATA+CGG	+	chr3_4:68380269-68380288	None:intergenic	60.0%
	CGGATACAGAATCCCCTCCG+AGG	+	chr3_4:68380656-68380675	None:intergenic	60.0%
	GTATCTGCACCCGCTGCTGG+TGG	-	chr3_4:68380268-68380287	Msa0516830:intron	65.0%
	GTATCTGCACCCGCTGCTGG+TGG	-	chr3_4:68380268-68380287	Msa0516830:intron	65.0%
	AGCGGCACCACCAACAGCGC+CGG	+	chr3_4:68380242-68380261	None:intergenic	70.0%
	GCCGTATCTGCACCCGCTGC+TGG	-	chr3_4:68380265-68380284	Msa0516830:intron	70.0%
	TCTGCACCCGCTGCTGGTGG+TGG	-	chr3_4:68380271-68380290	Msa0516830:intron	70.0%
	TGCGGCTCCACCACCAGCAG+CGG	+	chr3_4:68380281-68380300	None:intergenic	70.0%
	GGCAGGTTCGGCAGCTGCTG+CGG	+	chr3_4:68380299-68380318	None:intergenic	70.0%
	AGCGGCACCACCAACAGCGC+CGG	+	chr3_4:68380242-68380261	None:intergenic	70.0%
	GCCGTATCTGCACCCGCTGC+TGG	-	chr3_4:68380265-68380284	Msa0516830:intron	70.0%
	TCTGCACCCGCTGCTGGTGG+TGG	-	chr3_4:68380271-68380290	Msa0516830:intron	70.0%
	TGCGGCTCCACCACCAGCAG+CGG	+	chr3_4:68380281-68380300	None:intergenic	70.0%
	GGCAGGTTCGGCAGCTGCTG+CGG	+	chr3_4:68380299-68380318	None:intergenic	70.0%
	GCGGCTCCACCACCAGCAGC+GGG	+	chr3_4:68380280-68380299	None:intergenic	75.0%
	GCGGCTCCACCACCAGCAGC+GGG	+	chr3_4:68380280-68380299	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_4	gene	68379836	68381550	68379836	ID=Msa0516830;Name=Msa0516830
chr3_4	mRNA	68379836	68381550	68379836	ID=Msa0516830-mRNA-1;Parent=Msa0516830;Name=Msa0516830-mRNA-1;_AED=0.20;_eAED=0.20;_QI=120\|1\|1\|1\|1\|1\|3\|239\|110
chr3_4	exon	68379836	68380128	68379836	ID=Msa0516830-mRNA-1:exon:18018;Parent=Msa0516830-mRNA-1
chr3_4	exon	68381053	68381262	68381053	ID=Msa0516830-mRNA-1:exon:18017;Parent=Msa0516830-mRNA-1
chr3_4	exon	68381362	68381550	68381362	ID=Msa0516830-mRNA-1:exon:18016;Parent=Msa0516830-mRNA-1
chr3_4	five_prime_UTR	68381431	68381550	68381431	ID=Msa0516830-mRNA-1:five_prime_utr;Parent=Msa0516830-mRNA-1
chr3_4	CDS	68381362	68381430	68381362	ID=Msa0516830-mRNA-1:cds;Parent=Msa0516830-mRNA-1
chr3_4	CDS	68381053	68381262	68381053	ID=Msa0516830-mRNA-1:cds;Parent=Msa0516830-mRNA-1
chr3_4	CDS	68380075	68380128	68380075	ID=Msa0516830-mRNA-1:cds;Parent=Msa0516830-mRNA-1
chr3_4	three_prime_UTR	68379836	68380074	68379836	ID=Msa0516830-mRNA-1:three_prime_utr;Parent=Msa0516830-mRNA-1

Gene Sequence	>Msa0516830 ATGAGTTCCGGCGAATTGGGCTGCATTTACGCCACCTTGATCCTCCACGACGATGGAATCCCAATCACCGCGGAGAAGATCGGTACTTTGTTGAAGGCTGCTAACGTCACCGTTGAATCTTACTGGCCAAGCCTATTCGCTAAGCTTGCTCAGAGCAAGAACGTTGATGATCTCGTTTTGAACTCCGGCGCTGTTGGTGGTGCCGCTGTTGCCGTATCTGCACCCGCTGCTGGTGGTGGAGCCGCAGCAGCTGCCGAACCTGCCGCCGTTGAAAAGAAGGAGGAAGCCAAGGAAGAAAGTGACGATGACATGGGCTTTAGTTTGTTTGATTAA
Protein sequence	>Msa0516830 MSSGELGCIYATLILHDDGIPITAEKIGTLLKAANVTVESYWPSLFAKLAQSKNVDDLVLNSGAVGGAAVAVSAPAAGGGAAAAAEPAAVEKKEEAKEESDDDMGFSLFD*