AlfalfaGEDB | Msa1174110

M. sativa cultivar zhongmu-4 / Msa1174110

KEGG: -
GO: GO:0002376 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005911 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0009266 GO:0009409 GO:0009506 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009627 GO:0009628 GO:0009631 GO:0009682 GO:0009707 GO:0009814 GO:0009941 GO:0009987 GO:0012505 GO:0016020 GO:0019867 GO:0030054 GO:0031090 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0033554 GO:0042170 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043621 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0048046 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0070417 GO:0098542 GO:0098588 GO:0098805
PFAM: PF14547.9

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1174110	sp\|Q9SU33\|ERLL3_ARATH	46.753	77	37	2	44	116	101	177	7.25e-15	69.7

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1174110	A0A2K3MY61	55.556	117	47	2	1	115	1	114	4.13e-30	113

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0691160	Msa1174110	0.805364	1.383617e-49	-8.615850e-47
Msa0206800	Msa1174110	0.807143	5.835900e-50	-8.615850e-47
Msa0236110	Msa1174110	0.806229	9.104584e-50	-8.615850e-47
Msa0371540	Msa1174110	0.800267	1.561638e-48	-8.615850e-47
Msa0239440	Msa1174110	0.821826	3.285011e-53	-8.615850e-47
Msa0277670	Msa1174110	0.861732	8.340547e-64	-8.615850e-47
Msa0277720	Msa1174110	0.823204	1.571159e-53	-8.615850e-47
Msa0288200	Msa1174110	0.805510	1.289527e-49	-8.615850e-47
Msa0311790	Msa1174110	0.994259	8.261488e-206	-8.615850e-47
Msa0311810	Msa1174110	0.836245	1.047544e-56	-8.615850e-47
Msa0314630	Msa1174110	0.803981	2.688599e-49	-8.615850e-47
Msa0326540	Msa1174110	0.803334	3.662570e-49	-8.615850e-47
Msa1174110	Msa1292040	0.811970	5.363791e-51	-8.615850e-47
Msa1174110	Msa1345400	0.823750	1.171063e-53	-8.615850e-47
Msa0517340	Msa1174110	0.802909	4.484626e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1174110	MtrunA17_Chr1g0149481	42.857	91	43	4	29	111	70	159	2.96e-14	65.1
Msa1174110	MtrunA17_Chr1g0149491	46.250	80	34	4	40	111	68	146	3.03e-14	64.7
Msa1174110	MtrunA17_Chr4g0056411	43.662	71	36	2	44	110	93	163	1.18e-13	63.5
Msa1174110	MtrunA17_Chr1g0149471	48.611	72	32	3	44	111	135	205	1.39e-13	63.9
Msa1174110	MtrunA17_Chr4g0056391	41.667	72	38	2	44	111	67	138	2.67e-13	62.0
Msa1174110	MtrunA17_Chr4g0056401	44.444	72	36	2	44	111	58	129	5.09e-13	61.2
Msa1174110	MtrunA17_Chr2g0290521	40.860	93	43	3	18	98	16	108	4.82e-12	58.5
Msa1174110	MtrunA17_Chr3g0137521	36.508	126	62	6	1	110	1	124	6.92e-11	55.5
Msa1174110	MtrunA17_Chr4g0056371	41.860	86	41	4	39	116	48	132	9.20e-11	55.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1174110	AT5G46900.1	39.344	122	58	4	5	111	2	122	1.60e-18	75.5
Msa1174110	AT5G46890.1	37.705	122	60	4	5	111	2	122	2.90e-17	72.0
Msa1174110	AT4G12500.1	46.753	77	37	2	44	116	101	177	7.37e-16	69.7
Msa1174110	AT4G12470.1	46.479	71	34	2	44	110	85	155	2.69e-15	68.2
Msa1174110	AT4G12490.1	49.296	71	32	2	44	110	106	176	4.52e-15	67.8
Msa1174110	AT4G12520.1	35.938	128	69	4	1	115	1	128	4.93e-15	66.6
Msa1174110	AT4G12510.1	35.938	128	69	4	1	115	1	128	4.93e-15	66.6
Msa1174110	AT4G12480.1	46.479	71	34	2	44	110	92	162	6.58e-15	67.0
Msa1174110	AT1G12090.1	43.662	71	36	2	44	110	62	132	1.89e-12	60.1
Msa1174110	AT1G12100.1	38.144	97	55	3	24	116	37	132	8.39e-12	58.2
Msa1174110	AT2G45180.1	35.385	130	64	5	1	111	1	129	8.83e-12	58.2
Msa1174110	AT1G62510.1	37.975	79	41	3	40	110	66	144	2.03e-11	57.8
Msa1174110	AT4G12545.1	37.143	105	51	6	12	110	7	102	3.60e-11	55.8
Msa1174110	AT4G12530.1	37.778	90	51	3	12	99	11	97	5.98e-11	55.8

Find 35 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACCTTGAATACTTCCTTTA+TGG	0.070526	8_1:+5181522	None:intergenic
TTCCTTTATGGGAACGTTAA+TGG	0.184558	8_1:+5181534	None:intergenic
GAAGAGGATGAGAATGTTAA+TGG	0.273547	8_1:+5181789	None:intergenic
AGTAATCAAGGAAAATGTTT+TGG	0.276850	8_1:-5181563	Msa1174110:CDS
TCCTTTATGGGAACGTTAAT+GGG	0.309759	8_1:+5181535	None:intergenic
ACCTTGAATACTTCCTTTAT+GGG	0.339267	8_1:+5181523	None:intergenic
ACCCATTAACGTTCCCATAA+AGG	0.367765	8_1:-5181536	Msa1174110:CDS
GCCGGCACAAGCACTCAAAT+CGG	0.396560	8_1:+5181687	None:intergenic
CGCCTTAACAGAGTTAAGAT+TGG	0.406690	8_1:+5181744	None:intergenic
CACCAATCTTAACTCTGTTA+AGG	0.416695	8_1:-5181746	Msa1174110:CDS
TCCCATAAAGGAAGTATTCA+AGG	0.434770	8_1:-5181524	Msa1174110:CDS
TATTGCAAAATGGGTTCAAA+GGG	0.459238	8_1:-5181820	None:intergenic
TTCTACAAGACCACCGATGA+TGG	0.461353	8_1:+5181612	None:intergenic
CATATTACAAGGGCTTGTTA+AGG	0.462777	8_1:-5181665	Msa1174110:CDS
TGAAGAAGAAGAAGATGCTA+AGG	0.465305	8_1:+5181767	None:intergenic
TTATTGCAAAATGGGTTCAA+AGG	0.479552	8_1:-5181821	None:intergenic
CCCGATTTGAGTGCTTGTGC+CGG	0.487660	8_1:-5181688	Msa1174110:CDS
GCTTGTGCCGGCATATTACA+AGG	0.494276	8_1:-5181676	Msa1174110:CDS
AAATCGGGACACTGCGGCAG+TGG	0.500290	8_1:+5181703	None:intergenic
TTACAAGGGCTTGTTAAGGT+TGG	0.517501	8_1:-5181661	Msa1174110:CDS
AGAAGAAGATGCTAAGGAAG+AGG	0.520181	8_1:+5181773	None:intergenic
TAACCGCATACGGAGACATT+GGG	0.521289	8_1:+5181484	None:intergenic
TATATGAAGTTAACCGCATA+CGG	0.528309	8_1:+5181474	None:intergenic
AACAAGCCCTTGTAATATGC+CGG	0.532336	8_1:+5181669	None:intergenic
CCGGCACAAGCACTCAAATC+GGG	0.536967	8_1:+5181688	None:intergenic
GTGCCTTTGTGCAGTAATCA+AGG	0.543284	8_1:-5181575	Msa1174110:CDS
GGACACTGCGGCAGTGGTGA+TGG	0.554933	8_1:+5181709	None:intergenic
AAGTCTTGTTGCTCCATCAT+CGG	0.569193	8_1:-5181625	Msa1174110:CDS
GAGCAACAAGACTTTGCTTG+TGG	0.570002	8_1:+5181634	None:intergenic
TTAACCGCATACGGAGACAT+TGG	0.573421	8_1:+5181483	None:intergenic
GCACCCAATGTCTCCGTATG+CGG	0.626914	8_1:-5181487	Msa1174110:CDS
TCTTGTTGCTCCATCATCGG+TGG	0.639422	8_1:-5181622	Msa1174110:CDS
TTTCCTTGATTACTGCACAA+AGG	0.652604	8_1:+5181572	None:intergenic
CTTGTGCCGGCATATTACAA+GGG	0.672505	8_1:-5181675	Msa1174110:CDS
GCACTCAAATCGGGACACTG+CGG	0.725988	8_1:+5181697	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AGTAATCAAGGAAAATGTTT+TGG	-	chr8_1:5181731-5181750	Msa1174110:CDS	25.0%
!!	GTAATCAAGGAAAATGTTTT+GGG	-	chr8_1:5181732-5181751	Msa1174110:CDS	25.0%
	ACCTTGAATACTTCCTTTAT+GGG	+	chr8_1:5181774-5181793	None:intergenic	30.0%
	AACCTTGAATACTTCCTTTA+TGG	+	chr8_1:5181775-5181794	None:intergenic	30.0%
	GAAGAGGATGAGAATGTTAA+TGG	+	chr8_1:5181508-5181527	None:intergenic	35.0%
	TGAAGAAGAAGAAGATGCTA+AGG	+	chr8_1:5181530-5181549	None:intergenic	35.0%
	CACCAATCTTAACTCTGTTA+AGG	-	chr8_1:5181548-5181567	Msa1174110:CDS	35.0%
!!	CATATTACAAGGGCTTGTTA+AGG	-	chr8_1:5181629-5181648	Msa1174110:CDS	35.0%
	TTTCCTTGATTACTGCACAA+AGG	+	chr8_1:5181725-5181744	None:intergenic	35.0%
	TCCTTTATGGGAACGTTAAT+GGG	+	chr8_1:5181762-5181781	None:intergenic	35.0%
	TTCCTTTATGGGAACGTTAA+TGG	+	chr8_1:5181763-5181782	None:intergenic	35.0%
!!	TCCCATAAAGGAAGTATTCA+AGG	-	chr8_1:5181770-5181789	Msa1174110:CDS	35.0%
	AGAAGAAGATGCTAAGGAAG+AGG	+	chr8_1:5181524-5181543	None:intergenic	40.0%
	CGCCTTAACAGAGTTAAGAT+TGG	+	chr8_1:5181553-5181572	None:intergenic	40.0%
	AACAAGCCCTTGTAATATGC+CGG	+	chr8_1:5181628-5181647	None:intergenic	40.0%
!!	TTACAAGGGCTTGTTAAGGT+TGG	-	chr8_1:5181633-5181652	Msa1174110:CDS	40.0%
!!	AAGTCTTGTTGCTCCATCAT+CGG	-	chr8_1:5181669-5181688	Msa1174110:CDS	40.0%
	ACCCATTAACGTTCCCATAA+AGG	-	chr8_1:5181758-5181777	Msa1174110:CDS	40.0%
!	CTTGTGCCGGCATATTACAA+GGG	-	chr8_1:5181619-5181638	Msa1174110:CDS	45.0%
	GAGCAACAAGACTTTGCTTG+TGG	+	chr8_1:5181663-5181682	None:intergenic	45.0%
	TTCTACAAGACCACCGATGA+TGG	+	chr8_1:5181685-5181704	None:intergenic	45.0%
	GTGCCTTTGTGCAGTAATCA+AGG	-	chr8_1:5181719-5181738	Msa1174110:CDS	45.0%
	TAACCGCATACGGAGACATT+GGG	+	chr8_1:5181813-5181832	None:intergenic	45.0%
	TTAACCGCATACGGAGACAT+TGG	+	chr8_1:5181814-5181833	None:intergenic	45.0%
!	GCTTGTGCCGGCATATTACA+AGG	-	chr8_1:5181618-5181637	Msa1174110:CDS	50.0%
	TCTTGTTGCTCCATCATCGG+TGG	-	chr8_1:5181672-5181691	Msa1174110:CDS	50.0%
	GCACTCAAATCGGGACACTG+CGG	+	chr8_1:5181600-5181619	None:intergenic	55.0%
	CCGGCACAAGCACTCAAATC+GGG	+	chr8_1:5181609-5181628	None:intergenic	55.0%
!	CCCGATTTGAGTGCTTGTGC+CGG	-	chr8_1:5181606-5181625	Msa1174110:CDS	55.0%
	GCCGGCACAAGCACTCAAAT+CGG	+	chr8_1:5181610-5181629	None:intergenic	55.0%
	GCACCCAATGTCTCCGTATG+CGG	-	chr8_1:5181807-5181826	Msa1174110:CDS	55.0%
	AAATCGGGACACTGCGGCAG+TGG	+	chr8_1:5181594-5181613	None:intergenic	60.0%
	GGACACTGCGGCAGTGGTGA+TGG	+	chr8_1:5181588-5181607	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	5181483	5181833	5181483	ID=Msa1174110;Name=Msa1174110
chr8_1	mRNA	5181483	5181833	5181483	ID=Msa1174110-mRNA-1;Parent=Msa1174110;Name=Msa1174110-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|116
chr8_1	exon	5181483	5181833	5181483	ID=Msa1174110-mRNA-1:exon:1745;Parent=Msa1174110-mRNA-1
chr8_1	CDS	5181483	5181833	5181483	ID=Msa1174110-mRNA-1:cds;Parent=Msa1174110-mRNA-1

Gene Sequence	>Msa1174110 ATGGGTTCAAAGGGTTTTGCATCCATTAACATTCTCATCCTCTTCCTTAGCATCTTCTTCTTCTTCACCAATCTTAACTCTGTTAAGGCGCAAATCGCTTCACCATCACCACTGCCGCAGTGTCCCGATTTGAGTGCTTGTGCCGGCATATTACAAGGGCTTGTTAAGGTTGGTTCTCCACAAGCAAAGTCTTGTTGCTCCATCATCGGTGGTCTTGTAGAAGTTGATGCTGTTGTGTGCCTTTGTGCAGTAATCAAGGAAAATGTTTTGGGAATACCCATTAACGTTCCCATAAAGGAAGTATTCAAGGTTTGTGAACGTGAAGCACCCAATGTCTCCGTATGCGGTTAA
Protein sequence	>Msa1174110 MGSKGFASINILILFLSIFFFFTNLNSVKAQIASPSPLPQCPDLSACAGILQGLVKVGSPQAKSCCSIIGGLVEVDAVVCLCAVIKENVLGIPINVPIKEVFKVCEREAPNVSVCG*