AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1196110

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1196110 MtrunA17_Chr4g0053351 97.917 192 4 0 1 192 1 192 1.08e-140 390
Msa1196110 MtrunA17_Chr5g0401781 73.298 191 49 2 1 189 15 205 1.64e-100 289
Msa1196110 MtrunA17_Chr4g0035151 69.714 175 53 0 3 177 7 181 1.20e-88 258
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1196110 AT4G39220.2 69.780 182 55 0 4 185 7 188 3.28e-95 275
Msa1196110 AT4G39220.1 69.780 182 55 0 4 185 7 188 3.28e-95 275
Msa1196110 AT2G21600.1 66.845 187 61 1 4 190 7 192 6.80e-91 264
Msa1196110 AT2G23310.1 64.088 181 64 1 8 188 33 212 2.70e-84 248
Msa1196110 AT2G23310.2 64.088 181 63 2 8 188 33 211 2.42e-82 243
Msa1196110 AT2G18240.2 59.538 173 69 1 2 174 9 180 2.53e-72 218
Msa1196110 AT2G18240.1 59.538 173 69 1 2 174 9 180 2.61e-72 218

Find 62 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTCCGTCGCTTCCCACTTC+TGG 0.267512 8_1:+44849686 Msa1196110:CDS
TTGTTAGACTTTGGACTTCC+TGG 0.268588 8_1:+44851780 Msa1196110:three_prime_UTR
TCCATTCTCGTTTGGAAAAC+AGG 0.304741 8_1:+44851408 Msa1196110:CDS
GGCCAATACTCCTCTTCTAC+TGG 0.310598 8_1:+44851323 Msa1196110:CDS
CATGTAGCTTGAGTAAATTT+TGG 0.341383 8_1:+44851666 Msa1196110:three_prime_UTR
AAATATGTTCCATTCTCGTT+TGG 0.361766 8_1:+44851400 Msa1196110:CDS
CATTTGATGTCCCCGTGTTC+TGG 0.367605 8_1:+44851302 Msa1196110:CDS
TACGTTGTTTCATACGGTCT+TGG 0.378662 8_1:+44849590 Msa1196110:CDS
TGAGATATTGGGGATTATAT+CGG 0.381522 8_1:+44851842 Msa1196110:three_prime_UTR
AGCAGAAAGCACTAGCCTTT+CGG 0.398829 8_1:+44851585 Msa1196110:CDS
GGGTTGCCTTGTGGTTGCTT+TGG 0.399735 8_1:+44849532 Msa1196110:CDS
GATTGGATTTCTTTCGCCTC+AGG 0.407789 8_1:+44849634 Msa1196110:CDS
GAGATTCTTGATGCTGATAA+TGG 0.407935 8_1:+44849665 Msa1196110:CDS
CACCGTGTGATGGCGGCTGC+CGG 0.416331 8_1:-44849437 None:intergenic
TCAGGAAGACGACGAACAAA+AGG 0.428654 8_1:-44849725 None:intergenic
CTAGTAAAGGATTTAAACTT+GGG 0.443187 8_1:+44851817 Msa1196110:three_prime_UTR
TCTAGTAAAGGATTTAAACT+TGG 0.454456 8_1:+44851816 Msa1196110:three_prime_UTR
GTCTTCCTGAGTTTAAGTTC+TGG 0.457858 8_1:+44849738 Msa1196110:CDS
GGCTTCTACGTTGTTTCATA+CGG 0.457939 8_1:+44849584 Msa1196110:CDS
TATTGATTCAAACACAAAGA+TGG 0.458179 8_1:+44849397 Msa1196110:exon
TTGTGTCTAGTGTGGACAAC+TGG 0.459590 8_1:+44851734 Msa1196110:three_prime_UTR
TCCTCATAGTAAGCGTGAAT+AGG 0.475122 8_1:-44851351 None:intergenic
GCGAAAGAAATCCAATCAAA+AGG 0.486534 8_1:-44849628 None:intergenic
ATTATCAGCATCAAGAATCT+CGG 0.496258 8_1:-44849663 None:intergenic
GCCAATACTCCTCTTCTACT+GGG 0.520875 8_1:+44851324 Msa1196110:CDS
ACCCAGTAGAAGAGGAGTAT+TGG 0.533671 8_1:-44851325 None:intergenic
TTATCAGCATCAAGAATCTC+GGG 0.534119 8_1:-44849662 None:intergenic
TTCGAATTTCCACCGTGTGA+TGG 0.542286 8_1:-44849447 None:intergenic
CTTCGCGTTTACATCGTGCA+AGG 0.553353 8_1:+44849563 Msa1196110:CDS
AGACACAAAGTAACCTCTCT+CGG 0.559132 8_1:-44851719 None:intergenic
AGGAGTATTGGCCAGAACAC+GGG 0.567279 8_1:-44851313 None:intergenic
AATTACCAGAACTTAAACTC+AGG 0.568313 8_1:-44849743 None:intergenic
TCCTATTCACGCTTACTATG+AGG 0.569867 8_1:+44851350 Msa1196110:CDS
CTCCGGCAGCCGCCATCACA+CGG 0.572847 8_1:+44849435 Msa1196110:CDS
GGACACGGAAGACGCAACTC+CGG 0.578701 8_1:+44849418 Msa1196110:CDS
TTGCGGTTCGATATTAGTCG+TGG 0.579154 8_1:-44849336 None:intergenic
AAACTCATCAGAACCAGAAG+TGG 0.587504 8_1:-44849699 None:intergenic
ATAGGACCACCCAGTAGAAG+AGG 0.589643 8_1:-44851333 None:intergenic
GAACCAGAAGTGGGAAGCGA+CGG 0.596838 8_1:-44849689 None:intergenic
TTGATTTGTCGAGAATGTGT+TGG 0.600419 8_1:-44849484 None:intergenic
GAGGAGTATTGGCCAGAACA+CGG 0.604588 8_1:-44851314 None:intergenic
AGGTTACTTTGTGTCTAGTG+TGG 0.607621 8_1:+44851726 Msa1196110:three_prime_UTR
GAATTTCCACCGTGTGATGG+CGG 0.609513 8_1:-44849444 None:intergenic
AAATTTACTCAAGCTACATG+TGG 0.612172 8_1:-44851663 None:intergenic
GATGTGATGGCGTCCGAGAG+AGG 0.616618 8_1:+44851706 Msa1196110:three_prime_UTR
TAGTAAAGGATTTAAACTTG+GGG 0.617386 8_1:+44851818 Msa1196110:three_prime_UTR
ACGATGGCTGGGTTGCCTTG+TGG 0.628511 8_1:+44849523 Msa1196110:CDS
CGTAAACCAAAGCAACCACA+AGG 0.637103 8_1:-44849538 None:intergenic
AATTTACTCAAGCTACATGT+GGG 0.638260 8_1:-44851662 None:intergenic
AGCCATCGTTGACCGACGTG+CGG 0.644340 8_1:-44849509 None:intergenic
TGGAGCACATGAAGATGTGA+TGG 0.648505 8_1:+44851693 Msa1196110:three_prime_UTR
AACTCATCAGAACCAGAAGT+GGG 0.657734 8_1:-44849698 None:intergenic
GGAGTATTGGCCAGAACACG+GGG 0.660301 8_1:-44851312 None:intergenic
AATACTCCTCTTCTACTGGG+TGG 0.662233 8_1:+44851327 Msa1196110:CDS
GGTGGAAATTCGAAGCATCA+CGG 0.664894 8_1:+44849456 Msa1196110:CDS
GACAAATCAACGCCGCACGT+CGG 0.671907 8_1:+44849497 Msa1196110:CDS
AGTAAAGGATTTAAACTTGG+GGG 0.688398 8_1:+44851819 Msa1196110:three_prime_UTR
TTCAAACACAAAGATGGACA+CGG 0.691642 8_1:+44849403 Msa1196110:exon
AGAATCTCGGGATCAACCTG+AGG 0.697434 8_1:-44849650 None:intergenic
CGGCAGCCGCCATCACACGG+TGG 0.760978 8_1:+44849438 Msa1196110:CDS
GAACAGGTACTCAATCACGA+AGG 0.777688 8_1:+44851243 Msa1196110:intron
GGAAATTCGAAGCATCACGG+CGG 0.807446 8_1:+44849459 Msa1196110:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATTAAATATTGTTGAAT+TGG + chr8_1:44849864-44849883 Msa1196110:intron 10.0%
!!! GTATTTTTTATGTTAAAAAA+TGG - chr8_1:44851129-44851148 None:intergenic 10.0%
!! TATAAAATATTGTGAAATAG+TGG - chr8_1:44850316-44850335 None:intergenic 15.0%
!! AAATTATGAATTGTTCAAAA+CGG + chr8_1:44850939-44850958 Msa1196110:intron 15.0%
!! ATTCGTTACTTATTTGATTA+AGG + chr8_1:44849922-44849941 Msa1196110:intron 20.0%
!! CAGGTATTAATTGTAAAAAA+TGG - chr8_1:44850174-44850193 None:intergenic 20.0%
!! AAACATTTAACATTTACAAC+AGG - chr8_1:44850622-44850641 None:intergenic 20.0%
!!! TCTTAGTTTCTTATTTACAT+TGG + chr8_1:44851202-44851221 Msa1196110:intron 20.0%
!! AATATCTATAACAACTACTA+TGG - chr8_1:44851455-44851474 None:intergenic 20.0%
!!! ATATGATTACATCTTTGTTT+CGG + chr8_1:44851503-44851522 Msa1196110:intron 20.0%
!!! TGTTTTTTAAACTCTAGTAA+AGG + chr8_1:44851804-44851823 Msa1196110:three_prime_UTR 20.0%
!!! TATTGATTCAAACACAAAGA+TGG + chr8_1:44849397-44849416 Msa1196110:exon 25.0%
!! TATATCTTGAACCTTTTGAT+TGG + chr8_1:44849617-44849636 Msa1196110:CDS 25.0%
! AGATGAAATTAGAGTTAGAT+AGG + chr8_1:44849838-44849857 Msa1196110:intron 25.0%
!! TGTGTAAAGAAATCTGAAAT+TGG + chr8_1:44849954-44849973 Msa1196110:intron 25.0%
! TTAAACGCTAAAACAGTTAA+TGG - chr8_1:44850573-44850592 None:intergenic 25.0%
!! AAATCGTAAACTTGTTTCTT+TGG - chr8_1:44850685-44850704 None:intergenic 25.0%
! AAACAAGTTTACGATTTCTT+TGG + chr8_1:44850688-44850707 Msa1196110:intron 25.0%
! AACAAGTTTACGATTTCTTT+GGG + chr8_1:44850689-44850708 Msa1196110:intron 25.0%
! AATGAATACCAAAGAATGTA+GGG - chr8_1:44850723-44850742 None:intergenic 25.0%
! TGGTATTCATTGATATTTAC+AGG + chr8_1:44850732-44850751 Msa1196110:intron 25.0%
! GGTATTCATTGATATTTACA+GGG + chr8_1:44850733-44850752 Msa1196110:intron 25.0%
!! TGTAATTCTAATTTGTACTG+TGG - chr8_1:44850965-44850984 None:intergenic 25.0%
! AACATAAAAAATACGCCATT+TGG + chr8_1:44851135-44851154 Msa1196110:intron 25.0%
! TGTTTCGGATGATAAATATT+TGG + chr8_1:44851518-44851537 Msa1196110:intron 25.0%
!!! TTTTATTTTAGCGCTATGAT+CGG + chr8_1:44851551-44851570 Msa1196110:intron 25.0%
!!! AGTCTAACAATTTTTCAACT+AGG - chr8_1:44851770-44851789 None:intergenic 25.0%
! GTTGAAAAATTGTTAGACTT+TGG + chr8_1:44851771-44851790 Msa1196110:three_prime_UTR 25.0%
! TCTAGTAAAGGATTTAAACT+TGG + chr8_1:44851816-44851835 Msa1196110:three_prime_UTR 25.0%
! CTAGTAAAGGATTTAAACTT+GGG + chr8_1:44851817-44851836 Msa1196110:three_prime_UTR 25.0%
! TAGTAAAGGATTTAAACTTG+GGG + chr8_1:44851818-44851837 Msa1196110:three_prime_UTR 25.0%
!! GTTTTATTGCTTTCAGATGT+TGG - chr8_1:44849293-44849312 None:intergenic 30.0%
!! CCATTTTCTCGGTTTTTTTT+GGG - chr8_1:44849315-44849334 None:intergenic 30.0%
!! ACCATTTTCTCGGTTTTTTT+TGG - chr8_1:44849316-44849335 None:intergenic 30.0%
ATTATCAGCATCAAGAATCT+CGG - chr8_1:44849666-44849685 None:intergenic 30.0%
AATTACCAGAACTTAAACTC+AGG - chr8_1:44849746-44849765 None:intergenic 30.0%
TCTGGTAATTTCTACTTTCT+AGG + chr8_1:44849756-44849775 Msa1196110:intron 30.0%
CTGGTAATTTCTACTTTCTA+GGG + chr8_1:44849757-44849776 Msa1196110:intron 30.0%
!! ACTTTTCTTGTTTGTGATTG+TGG + chr8_1:44849808-44849827 Msa1196110:intron 30.0%
GTTGAATTGGTTAGATGAAA+TGG + chr8_1:44849877-44849896 Msa1196110:intron 30.0%
! TTGGTTAGATGAAATGGTTT+TGG + chr8_1:44849883-44849902 Msa1196110:intron 30.0%
TTATACTAAATAGCGTGTTG+CGG + chr8_1:44850331-44850350 Msa1196110:intron 30.0%
TTATTCAATTACAGAGGAAG+CGG - chr8_1:44850404-44850423 None:intergenic 30.0%
ATTGAATACCCTACATTCTT+TGG + chr8_1:44850712-44850731 Msa1196110:intron 30.0%
CAATGAATACCAAAGAATGT+AGG - chr8_1:44850724-44850743 None:intergenic 30.0%
!! ACTGATGTTGTGTTATTTAC+AGG + chr8_1:44850775-44850794 Msa1196110:intron 30.0%
!! CTGATGTTGTGTTATTTACA+GGG + chr8_1:44850776-44850795 Msa1196110:intron 30.0%
ATATGTGACAATTAAAGCTC+TGG + chr8_1:44850988-44851007 Msa1196110:intron 30.0%
TGAAGAGAAGAAAATCCAAA+TGG - chr8_1:44851153-44851172 None:intergenic 30.0%
! ATTTGGATTTTCTTCTCTTC+AGG + chr8_1:44851152-44851171 Msa1196110:intron 30.0%
AAATATGTTCCATTCTCGTT+TGG + chr8_1:44851400-44851419 Msa1196110:CDS 30.0%
AATTTACTCAAGCTACATGT+GGG - chr8_1:44851665-44851684 None:intergenic 30.0%
AAATTTACTCAAGCTACATG+TGG - chr8_1:44851666-44851685 None:intergenic 30.0%
! CATGTAGCTTGAGTAAATTT+TGG + chr8_1:44851666-44851685 Msa1196110:three_prime_UTR 30.0%
CTAGAGTTTAAAAAACATCC+AGG - chr8_1:44851801-44851820 None:intergenic 30.0%
AGTAAAGGATTTAAACTTGG+GGG + chr8_1:44851819-44851838 Msa1196110:three_prime_UTR 30.0%
TGAGATATTGGGGATTATAT+CGG + chr8_1:44851842-44851861 Msa1196110:three_prime_UTR 30.0%
!! GTTGGTTCAAGTTTTACAAC+CGG - chr8_1:44849275-44849294 None:intergenic 35.0%
CCCAAAAAAAACCGAGAAAA+TGG + chr8_1:44849312-44849331 Msa1196110:five_prime_UTR 35.0%
TTCAAACACAAAGATGGACA+CGG + chr8_1:44849403-44849422 Msa1196110:exon 35.0%
!! TTGATTTGTCGAGAATGTGT+TGG - chr8_1:44849487-44849506 None:intergenic 35.0%
GCGAAAGAAATCCAATCAAA+AGG - chr8_1:44849631-44849650 None:intergenic 35.0%
TTATCAGCATCAAGAATCTC+GGG - chr8_1:44849665-44849684 None:intergenic 35.0%
GAGATTCTTGATGCTGATAA+TGG + chr8_1:44849665-44849684 Msa1196110:CDS 35.0%
! TTTCTTGTTTGTGATTGTGG+AGG + chr8_1:44849811-44849830 Msa1196110:intron 35.0%
GTGATTGTGTGACTTGAATT+TGG + chr8_1:44849989-44850008 Msa1196110:intron 35.0%
!!! TAGTTGCCACAACCATTTTT+TGG + chr8_1:44850084-44850103 Msa1196110:intron 35.0%
!! ACTGATTTGCAGATTCATAC+AGG - chr8_1:44850226-44850245 None:intergenic 35.0%
CAAATCAGTGTTGTCAATTG+TGG + chr8_1:44850237-44850256 Msa1196110:intron 35.0%
! GCAGTGTTATTCAATTACAG+AGG - chr8_1:44850410-44850429 None:intergenic 35.0%
ATGCGCTCTACAAAAATTGT+AGG + chr8_1:44850801-44850820 Msa1196110:intron 35.0%
AATAAGATTGTAGTCACGTG+TGG + chr8_1:44850832-44850851 Msa1196110:intron 35.0%
! AAATGTTTTCCTCTGTGAAC+AGG + chr8_1:44851227-44851246 Msa1196110:intron 35.0%
TGAAAAATCAGTTGTCCGAA+AGG - chr8_1:44851603-44851622 None:intergenic 35.0%
!! TTTCGGACAACTGATTTTTC+AGG + chr8_1:44851602-44851621 Msa1196110:exon 35.0%
! TTAGTCGTGGACCATTTTCT+CGG - chr8_1:44849326-44849345 None:intergenic 40.0%
! GGCTTCTACGTTGTTTCATA+CGG + chr8_1:44849584-44849603 Msa1196110:CDS 40.0%
! TACGTTGTTTCATACGGTCT+TGG + chr8_1:44849590-44849609 Msa1196110:CDS 40.0%
AACTCATCAGAACCAGAAGT+GGG - chr8_1:44849701-44849720 None:intergenic 40.0%
AAACTCATCAGAACCAGAAG+TGG - chr8_1:44849702-44849721 None:intergenic 40.0%
! GTCTTCCTGAGTTTAAGTTC+TGG + chr8_1:44849738-44849757 Msa1196110:CDS 40.0%
GTGATGTGATCTCTCGTATA+TGG + chr8_1:44850011-44850030 Msa1196110:intron 40.0%
! CATTACGCTGACTGAAAGAA+CGG + chr8_1:44850045-44850064 Msa1196110:intron 40.0%
ATGCTGCCAAAAAATGGTTG+TGG - chr8_1:44850093-44850112 None:intergenic 40.0%
CTGAAGATGCTGCCAAAAAA+TGG - chr8_1:44850099-44850118 None:intergenic 40.0%
AATGTCTACTGATGTCATGC+TGG + chr8_1:44850123-44850142 Msa1196110:intron 40.0%
AATTAATACCTGCTACAGCC+TGG + chr8_1:44850182-44850201 Msa1196110:intron 40.0%
TTGTGGACCATGGAAAATAG+CGG + chr8_1:44850254-44850273 Msa1196110:intron 40.0%
!! GAACAAACCGCTATTTTCCA+TGG - chr8_1:44850264-44850283 None:intergenic 40.0%
ACTGCTGCAAATACTCACTT+TGG + chr8_1:44850425-44850444 Msa1196110:intron 40.0%
TGAAAGAGCAAGTCTCTTGA+AGG + chr8_1:44850456-44850475 Msa1196110:intron 40.0%
GTCAGCTTAGACTAACAACA+TGG + chr8_1:44850515-44850534 Msa1196110:intron 40.0%
GGTGTGAGTGATGAAATTGA+GGG - chr8_1:44850601-44850620 None:intergenic 40.0%
AGGTGTGAGTGATGAAATTG+AGG - chr8_1:44850602-44850621 None:intergenic 40.0%
TCCTATTCACGCTTACTATG+AGG + chr8_1:44851350-44851369 Msa1196110:CDS 40.0%
TCCTCATAGTAAGCGTGAAT+AGG - chr8_1:44851354-44851373 None:intergenic 40.0%
TCCATTCTCGTTTGGAAAAC+AGG + chr8_1:44851408-44851427 Msa1196110:CDS 40.0%
! ACCTGTTTTCCAAACGAGAA+TGG - chr8_1:44851412-44851431 None:intergenic 40.0%
!! CTTGAGTAAATTTTGGCAGC+TGG + chr8_1:44851673-44851692 Msa1196110:three_prime_UTR 40.0%
AGACACAAAGTAACCTCTCT+CGG - chr8_1:44851722-44851741 None:intergenic 40.0%
! AGGTTACTTTGTGTCTAGTG+TGG + chr8_1:44851726-44851745 Msa1196110:three_prime_UTR 40.0%
TTGTTAGACTTTGGACTTCC+TGG + chr8_1:44851780-44851799 Msa1196110:three_prime_UTR 40.0%
TAAACTTGGGGGTGAGATAT+TGG + chr8_1:44851830-44851849 Msa1196110:three_prime_UTR 40.0%
AAACTTGGGGGTGAGATATT+GGG + chr8_1:44851831-44851850 Msa1196110:three_prime_UTR 40.0%
TTGCGGTTCGATATTAGTCG+TGG - chr8_1:44849339-44849358 None:intergenic 45.0%
TCTCTCTCTCTCTCAGTTTG+CGG - chr8_1:44849356-44849375 None:intergenic 45.0%
TTCGAATTTCCACCGTGTGA+TGG - chr8_1:44849450-44849469 None:intergenic 45.0%
! GGTGGAAATTCGAAGCATCA+CGG + chr8_1:44849456-44849475 Msa1196110:CDS 45.0%
CGTAAACCAAAGCAACCACA+AGG - chr8_1:44849541-44849560 None:intergenic 45.0%
GATTGGATTTCTTTCGCCTC+AGG + chr8_1:44849634-44849653 Msa1196110:CDS 45.0%
TCAGGAAGACGACGAACAAA+AGG - chr8_1:44849728-44849747 None:intergenic 45.0%
CTTGTTTGTGATTGTGGAGG+TGG + chr8_1:44849814-44849833 Msa1196110:intron 45.0%
GTGTTGTCAATTGTGGACCA+TGG + chr8_1:44850244-44850263 Msa1196110:intron 45.0%
AAGAGCAAGTCTCTTGAAGG+TGG + chr8_1:44850459-44850478 Msa1196110:intron 45.0%
TCTTGAAGGTGGTTCAGTCT+TGG + chr8_1:44850470-44850489 Msa1196110:intron 45.0%
CTTGAAGGTGGTTCAGTCTT+GGG + chr8_1:44850471-44850490 Msa1196110:intron 45.0%
! ACAACATGGACCATCCCTTT+TGG + chr8_1:44850529-44850548 Msa1196110:intron 45.0%
TAGTCACGTGTGGAACTGAA+AGG + chr8_1:44850842-44850861 Msa1196110:intron 45.0%
TGGAAAGAGATCTCGATGGA+CGG + chr8_1:44851008-44851027 Msa1196110:intron 45.0%
GCAAAGCAAAGCAAGCAGAA+AGG - chr8_1:44851074-44851093 None:intergenic 45.0%
GATTGAGTACCTGTTCACAG+AGG - chr8_1:44851239-44851258 None:intergenic 45.0%
GAACAGGTACTCAATCACGA+AGG + chr8_1:44851243-44851262 Msa1196110:intron 45.0%
GCCAATACTCCTCTTCTACT+GGG + chr8_1:44851324-44851343 Msa1196110:CDS 45.0%
! ACCCAGTAGAAGAGGAGTAT+TGG - chr8_1:44851328-44851347 None:intergenic 45.0%
AATACTCCTCTTCTACTGGG+TGG + chr8_1:44851327-44851346 Msa1196110:CDS 45.0%
!! AGCAGAAAGCACTAGCCTTT+CGG + chr8_1:44851585-44851604 Msa1196110:CDS 45.0%
TGGAGCACATGAAGATGTGA+TGG + chr8_1:44851693-44851712 Msa1196110:three_prime_UTR 45.0%
TTGTGTCTAGTGTGGACAAC+TGG + chr8_1:44851734-44851753 Msa1196110:three_prime_UTR 45.0%
AACTTGGGGGTGAGATATTG+GGG + chr8_1:44851832-44851851 Msa1196110:three_prime_UTR 45.0%
GAATTTCCACCGTGTGATGG+CGG - chr8_1:44849447-44849466 None:intergenic 50.0%
! GGAAATTCGAAGCATCACGG+CGG + chr8_1:44849459-44849478 Msa1196110:CDS 50.0%
CTTCGCGTTTACATCGTGCA+AGG + chr8_1:44849563-44849582 Msa1196110:CDS 50.0%
AGAATCTCGGGATCAACCTG+AGG - chr8_1:44849653-44849672 None:intergenic 50.0%
CACGAAACCTGTAGACTACC+AGG - chr8_1:44850203-44850222 None:intergenic 50.0%
ACAGTAGCACTACTGCGTAG+CGG - chr8_1:44850292-44850311 None:intergenic 50.0%
! ACATGGACCATCCCTTTTGG+CGG + chr8_1:44850532-44850551 Msa1196110:intron 50.0%
GCTTAGCTGAACCGCCAAAA+GGG - chr8_1:44850546-44850565 None:intergenic 50.0%
AGCTTAGCTGAACCGCCAAA+AGG - chr8_1:44850547-44850566 None:intergenic 50.0%
!! GCTCTGGAAAGAGATCTCGA+TGG + chr8_1:44851004-44851023 Msa1196110:intron 50.0%
CATTTGATGTCCCCGTGTTC+TGG + chr8_1:44851302-44851321 Msa1196110:CDS 50.0%
!! AGGAGTATTGGCCAGAACAC+GGG - chr8_1:44851316-44851335 None:intergenic 50.0%
!! GAGGAGTATTGGCCAGAACA+CGG - chr8_1:44851317-44851336 None:intergenic 50.0%
GGCCAATACTCCTCTTCTAC+TGG + chr8_1:44851323-44851342 Msa1196110:CDS 50.0%
ATAGGACCACCCAGTAGAAG+AGG - chr8_1:44851336-44851355 None:intergenic 50.0%
GACAAATCAACGCCGCACGT+CGG + chr8_1:44849497-44849516 Msa1196110:CDS 55.0%
GGGTTGCCTTGTGGTTGCTT+TGG + chr8_1:44849532-44849551 Msa1196110:CDS 55.0%
GAACCAGAAGTGGGAAGCGA+CGG - chr8_1:44849692-44849711 None:intergenic 55.0%
GTAGACTACCAGGCTGTAGC+AGG - chr8_1:44850193-44850212 None:intergenic 55.0%
GCTACAGCCTGGTAGTCTAC+AGG + chr8_1:44850193-44850212 Msa1196110:intron 55.0%
AGCTGAACCGCCAAAAGGGA+TGG - chr8_1:44850542-44850561 None:intergenic 55.0%
!! GGAGTATTGGCCAGAACACG+GGG - chr8_1:44851315-44851334 None:intergenic 55.0%
GGACACGGAAGACGCAACTC+CGG + chr8_1:44849418-44849437 Msa1196110:CDS 60.0%
AGCCATCGTTGACCGACGTG+CGG - chr8_1:44849512-44849531 None:intergenic 60.0%
CACGTCGGTCAACGATGGCT+GGG + chr8_1:44849512-44849531 Msa1196110:CDS 60.0%
ACGATGGCTGGGTTGCCTTG+TGG + chr8_1:44849523-44849542 Msa1196110:CDS 60.0%
! GATGTGATGGCGTCCGAGAG+AGG + chr8_1:44851706-44851725 Msa1196110:three_prime_UTR 60.0%
GCACGTCGGTCAACGATGGC+TGG + chr8_1:44849511-44849530 Msa1196110:CDS 65.0%
GGTCCGTCGCTTCCCACTTC+TGG + chr8_1:44849686-44849705 Msa1196110:CDS 65.0%
CTCCGGCAGCCGCCATCACA+CGG + chr8_1:44849435-44849454 Msa1196110:CDS 70.0%
!! CACCGTGTGATGGCGGCTGC+CGG - chr8_1:44849440-44849459 None:intergenic 70.0%
CGCCGCACGTCGGTCAACGA+TGG + chr8_1:44849507-44849526 Msa1196110:CDS 70.0%
CGGCAGCCGCCATCACACGG+TGG + chr8_1:44849438-44849457 Msa1196110:CDS 75.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 44849254 44851871 44849254 ID=Msa1196110;Name=Msa1196110
chr8_1 mRNA 44849254 44851871 44849254 ID=Msa1196110-mRNA-1;Parent=Msa1196110;Name=Msa1196110-mRNA-1;_AED=0.04;_eAED=0.04;_QI=162|1|1|1|1|1|3|256|192
chr8_1 exon 44849254 44849759 44849254 ID=Msa1196110-mRNA-1:exon:14417;Parent=Msa1196110-mRNA-1
chr8_1 exon 44851249 44851429 44851249 ID=Msa1196110-mRNA-1:exon:14418;Parent=Msa1196110-mRNA-1
chr8_1 exon 44851562 44851871 44851562 ID=Msa1196110-mRNA-1:exon:14419;Parent=Msa1196110-mRNA-1
chr8_1 five_prime_UTR 44849254 44849415 44849254 ID=Msa1196110-mRNA-1:five_prime_utr;Parent=Msa1196110-mRNA-1
chr8_1 CDS 44849416 44849759 44849416 ID=Msa1196110-mRNA-1:cds;Parent=Msa1196110-mRNA-1
chr8_1 CDS 44851249 44851429 44851249 ID=Msa1196110-mRNA-1:cds;Parent=Msa1196110-mRNA-1
chr8_1 CDS 44851562 44851615 44851562 ID=Msa1196110-mRNA-1:cds;Parent=Msa1196110-mRNA-1
chr8_1 three_prime_UTR 44851616 44851871 44851616 ID=Msa1196110-mRNA-1:three_prime_utr;Parent=Msa1196110-mRNA-1
Gene Sequence

>Msa1196110

ATGGACACGGAAGACGCAACTCCGGCAGCCGCCATCACACGGTGGAAATTCGAAGCATCACGGCGGTACCAACACATTCTCGACAAATCAACGCCGCACGTCGGTCAACGATGGCTGGGTTGCCTTGTGGTTGCTTTGGTTTACGTTCTTCGCGTTTACATCGTGCAAGGCTTCTACGTTGTTTCATACGGTCTTGGCATTTATATCTTGAACCTTTTGATTGGATTTCTTTCGCCTCAGGTTGATCCCGAGATTCTTGATGCTGATAATGGTCCGTCGCTTCCCACTTCTGGTTCTGATGAGTTTCGTCCTTTTGTTCGTCGTCTTCCTGAGTTTAAGTTCTGGTACTCAATCACGAAGGCATTTTGCATTGCATTTGTGATGACTTTCTTTAGTGCATTTGATGTCCCCGTGTTCTGGCCAATACTCCTCTTCTACTGGGTGGTCCTATTCACGCTTACTATGAGGAGACAGATAGCTCACATGATCAAGTACAAATATGTTCCATTCTCGTTTGGAAAACAGCGCTATGATCGGAAGAGAGCATCAGCAGAAAGCACTAGCCTTTCGGACAACTGA
Protein sequence

>Msa1196110

MDTEDATPAAAITRWKFEASRRYQHILDKSTPHVGQRWLGCLVVALVYVLRVYIVQGFYVVSYGLGIYILNLLIGFLSPQVDPEILDADNGPSLPTSGSDEFRPFVRRLPEFKFWYSITKAFCIAFVMTFFSAFDVPVFWPILLFYWVVLFTLTMRRQIAHMIKYKYVPFSFGKQRYDRKRASAESTSLSDN*