Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1199110 | KEH20243.1 | 77.586 | 58 | 13 | 0 | 1 | 58 | 1 | 58 | 7.96e-22 | 93.6 |
| Msa1199110 | KEH20243.1 | 82.143 | 84 | 15 | 0 | 60 | 143 | 1 | 84 | 7.61e-44 | 149 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0010150 | Msa1199110 | 0.802173 | 6.360839e-49 | -8.615850e-47 |
| Msa0059880 | Msa1199110 | 0.812380 | 4.365443e-51 | -8.615850e-47 |
| Msa0643840 | Msa1199110 | 0.801106 | 1.052764e-48 | -8.615850e-47 |
| Msa0646210 | Msa1199110 | 0.800752 | 1.243477e-48 | -8.615850e-47 |
| Msa0670360 | Msa1199110 | 0.800899 | 1.160833e-48 | -8.615850e-47 |
| Msa0814160 | Msa1199110 | 0.823928 | 1.063521e-53 | -8.615850e-47 |
| Msa0150910 | Msa1199110 | 0.807358 | 5.255940e-50 | -8.615850e-47 |
| Msa1061670 | Msa1199110 | 0.804161 | 2.467611e-49 | -8.615850e-47 |
| Msa1087380 | Msa1199110 | 0.801187 | 1.013299e-48 | -8.615850e-47 |
| Msa0272210 | Msa1199110 | 0.801456 | 8.928010e-49 | -8.615850e-47 |
| Msa1199110 | Msa1252630 | 0.800772 | 1.232242e-48 | -8.615850e-47 |
| Msa0535570 | Msa1199110 | 0.805147 | 1.535909e-49 | -8.615850e-47 |
| Msa0549930 | Msa1199110 | 0.806010 | 1.012358e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1199110 | MtrunA17_Chr8g0368881 | 82.143 | 84 | 15 | 0 | 60 | 143 | 1 | 84 | 7.00e-48 | 149 |
| Msa1199110 | MtrunA17_Chr8g0368881 | 77.586 | 58 | 13 | 0 | 1 | 58 | 1 | 58 | 7.32e-26 | 93.6 |
| Msa1199110 | MtrunA17_Chr4g0018981 | 51.163 | 86 | 32 | 3 | 60 | 143 | 1 | 78 | 2.32e-21 | 82.0 |
| Msa1199110 | MtrunA17_Chr4g0018981 | 56.364 | 55 | 23 | 1 | 1 | 55 | 1 | 54 | 3.32e-13 | 60.8 |
| Msa1199110 | MtrunA17_Chr4g0019001 | 52.941 | 85 | 25 | 3 | 60 | 143 | 1 | 71 | 1.12e-20 | 80.1 |
| Msa1199110 | MtrunA17_Chr4g0019021 | 43.243 | 111 | 30 | 4 | 60 | 143 | 1 | 105 | 1.42e-19 | 78.2 |
| Msa1199110 | MtrunA17_Chr4g0018961 | 47.619 | 84 | 35 | 2 | 60 | 143 | 1 | 75 | 1.61e-19 | 77.0 |
| Msa1199110 | MtrunA17_Chr4g0018961 | 62.000 | 50 | 18 | 1 | 1 | 50 | 1 | 49 | 8.47e-13 | 59.7 |
| Msa1199110 | MtrunA17_Chr4g0018931 | 42.529 | 87 | 38 | 3 | 60 | 143 | 1 | 78 | 8.89e-16 | 67.8 |
| Msa1199110 | MtrunA17_Chr4g0019361 | 47.619 | 84 | 34 | 3 | 60 | 143 | 1 | 74 | 1.91e-14 | 63.9 |
| Msa1199110 | MtrunA17_Chr4g0018971 | 43.023 | 86 | 35 | 3 | 60 | 142 | 1 | 75 | 7.62e-14 | 62.4 |
| Msa1199110 | MtrunA17_Chr4g0019351 | 40.594 | 101 | 38 | 3 | 60 | 143 | 1 | 96 | 1.77e-13 | 62.0 |
| Msa1199110 | MtrunA17_Chr4g0019351 | 56.863 | 51 | 22 | 0 | 1 | 51 | 1 | 51 | 1.71e-11 | 57.0 |
| Msa1199110 | MtrunA17_Chr4g0019341 | 39.394 | 99 | 42 | 3 | 60 | 143 | 1 | 96 | 3.82e-12 | 58.5 |
| Msa1199110 | MtrunA17_Chr4g0019341 | 56.863 | 51 | 22 | 0 | 1 | 51 | 1 | 51 | 2.32e-11 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 32 sgRNAs with CRISPR-Local
Find 2 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAGAGAACCAACCAAATA+AGG | 0.189852 | 8_1:-50966710 | None:intergenic |
| CTAAGAAGTGCTCCCTTATT+TGG | 0.192670 | 8_1:+50966697 | Msa1199110:CDS |
| CTAAGAAGTGCTCCCTTATT+TGG | 0.192670 | 8_1:+50970661 | Msa1199110:CDS |
| AAACGAGAACCAACCAAATA+AGG | 0.224741 | 8_1:-50970674 | None:intergenic |
| CCTCTCTTACATCGTTGATC+TGG | 0.247600 | 8_1:-50966970 | None:intergenic |
| TGTAGTTTCAGAAGAAATAA+TGG | 0.295791 | 8_1:+50971027 | Msa1199110:intron |
| GAAAGCCTTATCAAAGATAA+TGG | 0.392698 | 8_1:-50971162 | None:intergenic |
| CCTCTCTTACATCGTTGATC+TGG | 0.400600 | 8_1:-50971073 | None:intergenic |
| GTAGTTTCAGAAGAAATAAT+GGG | 0.401758 | 8_1:+50971028 | Msa1199110:intron |
| CTCTCTTACATCGTTGATCT+GGG | 0.408001 | 8_1:-50966969 | None:intergenic |
| TGCCACCATTATCTTTGATA+AGG | 0.416108 | 8_1:+50971157 | Msa1199110:exon |
| AACGAGAACCAACCAAATAA+GGG | 0.429145 | 8_1:-50970673 | None:intergenic |
| TTATTTGATGCCTTGCGATC+GGG | 0.437566 | 8_1:-50971127 | None:intergenic |
| AAAGAGAACCAACCAAATAA+GGG | 0.449521 | 8_1:-50966709 | None:intergenic |
| GTAGTTTCAGATGAAATAAC+GGG | 0.451726 | 8_1:+50966925 | Msa1199110:intron |
| TTTCTTGTGTTACAATTTCA+CGG | 0.454188 | 8_1:+50971181 | Msa1199110:three_prime_UTR |
| TGTAGTTTCAGATGAAATAA+CGG | 0.454757 | 8_1:+50966924 | Msa1199110:intron |
| GAAGTGCTCCCTTATTTGGT+TGG | 0.463673 | 8_1:+50966701 | Msa1199110:CDS |
| GAAGTGCTCCCTTATTTGGT+TGG | 0.463673 | 8_1:+50970665 | Msa1199110:CDS |
| ATTATTTGATGCCTTGCGAT+CGG | 0.486623 | 8_1:-50971128 | None:intergenic |
| TATGCTGAGTTTATCTGCCA+AGG | 0.501589 | 8_1:+50966737 | Msa1199110:CDS |
| AATGTTCCTTGCTATAGCCT+TGG | 0.522934 | 8_1:-50966754 | None:intergenic |
| AATGTTCCTTGCTATAGCCT+TGG | 0.522934 | 8_1:-50970718 | None:intergenic |
| TGAAGTTATGCCCGATCGCA+AGG | 0.538497 | 8_1:+50971117 | Msa1199110:CDS |
| AAAGACACATTGATTAGATG+TGG | 0.542737 | 8_1:+50971220 | Msa1199110:three_prime_UTR |
| TATGCTGAGTTTATCTGCCA+AGG | 0.598770 | 8_1:+50970701 | Msa1199110:CDS |
| CCAGATCAACGATGTAAGAG+AGG | 0.617840 | 8_1:+50971073 | Msa1199110:CDS |
| CAAAGATAATGGTGGCAACG+TGG | 0.632142 | 8_1:-50971151 | None:intergenic |
| AAGACACATTGATTAGATGT+GGG | 0.642402 | 8_1:+50971221 | Msa1199110:three_prime_UTR |
| AGCCTTATCAAAGATAATGG+TGG | 0.676453 | 8_1:-50971159 | None:intergenic |
| TATCTGCCAAGGCTATAGCA+AGG | 0.688081 | 8_1:+50966748 | Msa1199110:CDS |
| TATCTGCCAAGGCTATAGCA+AGG | 0.688081 | 8_1:+50970712 | Msa1199110:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTAAGAAGTGCTCCCTTATT+TGG | + | chr8_1:50966697-50966716 | Msa1199110:CDS | 40.0% |
| ! | GAAGTGCTCCCTTATTTGGT+TGG | + | chr8_1:50966701-50966720 | Msa1199110:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 50966690 | 50971243 | 50966690 | ID=Msa1199110;Name=Msa1199110 |
| chr8_1 | mRNA | 50966690 | 50971243 | 50966690 | ID=Msa1199110-mRNA-1;Parent=Msa1199110;Name=Msa1199110-mRNA-1;_AED=0.22;_eAED=0.22;_QI=0|0.66|0.25|1|1|1|4|69|143 |
| chr8_1 | exon | 50966690 | 50966801 | 50966690 | ID=Msa1199110-mRNA-1:exon:16083;Parent=Msa1199110-mRNA-1 |
| chr8_1 | exon | 50966929 | 50966982 | 50966929 | ID=Msa1199110-mRNA-1:exon:16084;Parent=Msa1199110-mRNA-1 |
| chr8_1 | exon | 50970643 | 50970765 | 50970643 | ID=Msa1199110-mRNA-1:exon:16085;Parent=Msa1199110-mRNA-1 |
| chr8_1 | exon | 50971032 | 50971243 | 50971032 | ID=Msa1199110-mRNA-1:exon:16086;Parent=Msa1199110-mRNA-1 |
| chr8_1 | CDS | 50966690 | 50966801 | 50966690 | ID=Msa1199110-mRNA-1:cds;Parent=Msa1199110-mRNA-1 |
| chr8_1 | CDS | 50966929 | 50966982 | 50966929 | ID=Msa1199110-mRNA-1:cds;Parent=Msa1199110-mRNA-1 |
| chr8_1 | CDS | 50970643 | 50970765 | 50970643 | ID=Msa1199110-mRNA-1:cds;Parent=Msa1199110-mRNA-1 |
| chr8_1 | CDS | 50971032 | 50971174 | 50971032 | ID=Msa1199110-mRNA-1:cds;Parent=Msa1199110-mRNA-1 |
| chr8_1 | three_prime_UTR | 50971175 | 50971243 | 50971175 | ID=Msa1199110-mRNA-1:three_prime_utr;Parent=Msa1199110-mRNA-1 |
| Gene Sequence |
| Protein sequence |
