AlfalfaGEDB | Msa1210850

M. sativa cultivar zhongmu-4 / Msa1210850

KEGG: K09377
GO: -
PFAM: PF00412.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1210850	sp\|Q94JX5\|WLIM1_ARATH	69.143	175	53	1	3	176	2	176	1.51e-89	263

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1210850	G7JNV0	95.580	181	8	0	1	181	1	181	7.90e-127	363

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TFs/TRs:

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Gene ID	Type	Classification
Msa1210850	TF	LIM

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0512640	Msa1210850	0.802607	5.177498e-49	-8.615850e-47
Msa0570600	Msa1210850	0.800274	1.556177e-48	-8.615850e-47
Msa1210150	Msa1210850	0.968963	1.981016e-129	-8.615850e-47
Msa1210250	Msa1210850	0.865322	6.425495e-65	-8.615850e-47
Msa1210850	Msa1259260	0.800005	1.765265e-48	-8.615850e-47
Msa1210850	Msa1396720	0.960312	2.047379e-118	-8.615850e-47
Msa1210850	Msa1425760	0.963315	6.183736e-122	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1210850	MtrunA17_Chr4g0059371	95.580	181	8	0	1	181	1	181	1.52e-130	363
Msa1210850	MtrunA17_Chr1g0152431	67.429	175	56	1	3	176	2	176	2.70e-89	259
Msa1210850	MtrunA17_Chr3g0113051	54.945	182	77	3	3	181	2	181	5.11e-69	208
Msa1210850	MtrunA17_Chr8g0386501	50.838	179	81	2	3	181	2	173	6.17e-62	191
Msa1210850	MtrunA17_Chr8g0386501	53.521	71	33	0	2	72	93	163	5.81e-20	83.2
Msa1210850	MtrunA17_Chr8g0349391	50.279	179	76	2	3	177	2	171	5.76e-60	185
Msa1210850	MtrunA17_Chr7g0247111	49.133	173	81	2	3	173	2	169	4.86e-58	181
Msa1210850	MtrunA17_Chr7g0247111	43.038	79	45	0	4	82	99	177	1.71e-15	71.2
Msa1210850	MtrunA17_Chr7g0247111	43.056	72	41	0	103	174	3	74	3.38e-15	70.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1210850	AT1G10200.1	69.143	175	53	1	3	176	2	176	1.53e-90	263
Msa1210850	AT3G55770.3	56.111	180	76	2	3	181	2	179	2.45e-70	212
Msa1210850	AT3G55770.5	56.111	180	76	2	3	181	2	179	2.45e-70	212
Msa1210850	AT3G55770.2	56.111	180	76	2	3	181	2	179	2.45e-70	212
Msa1210850	AT3G55770.1	56.111	180	76	2	3	181	2	179	2.45e-70	212
Msa1210850	AT2G39900.1	53.073	179	84	0	3	181	2	180	5.29e-69	208
Msa1210850	AT3G61230.1	53.179	173	78	1	1	173	1	170	8.14e-67	203
Msa1210850	AT3G61230.1	41.758	91	53	0	4	94	100	190	4.36e-18	78.6
Msa1210850	AT3G61230.1	44.444	72	40	0	103	174	4	75	6.22e-16	72.8
Msa1210850	AT1G01780.1	52.326	172	75	2	3	173	2	167	5.38e-63	193

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Find 41 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAGTGTACCCAATTGAAA+AGG	0.511293	8_1:+70073461	Msa1210850:CDS
AATCAGTCCATCAAACTATG+TGG	0.634185	8_1:+70074365	Msa1210850:CDS
AATGACACTGTTACAATACT+TGG	0.434857	8_1:+70074585	Msa1210850:three_prime_UTR
AATGTGTCTGAAATTAATGT+AGG	0.578011	8_1:+70071725	Msa1210850:five_prime_UTR
ACAGGTCGAAGCATATAACA+AGG	0.699921	8_1:+70073389	Msa1210850:intron
ACTATAATGCAACTTTGCAG+TGG	0.646927	8_1:+70074520	Msa1210850:three_prime_UTR
ACTCAGATCTTCAAACAGAA+GGG	0.607367	8_1:+70074423	Msa1210850:CDS
ACTTGCTTCAGGTGCACACA+TGG	0.555007	8_1:+70074333	Msa1210850:CDS
AGTAGTAATCATGTTCATAT+TGG	0.418531	8_1:-70071748	None:intergenic
AGTTGAAAGATCAACTGATC+AGG	0.484362	8_1:+70073281	Msa1210850:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTCTTTCAAATTCAGTGTAG+GGG	-	chr8_1:70071820-70071839	None:intergenic	30.0%
CATGTGAAAAGAAAGTTTAC+TGG	+	chr8_1:70071935-70071954	Msa1210850:CDS	30.0%
TTTCGATGAATGCTTCAATA+AGG	+	chr8_1:70072071-70072090	Msa1210850:intron	30.0%
ACATGTCATACGAATGTTAT+AGG	-	chr8_1:70072464-70072483	None:intergenic	30.0%
TTAGAAATGTTGATCAACGA+AGG	+	chr8_1:70072652-70072671	Msa1210850:intron	30.0%
TAGAAATGTTGATCAACGAA+GGG	+	chr8_1:70072653-70072672	Msa1210850:intron	30.0%
TTAAGCAATAACCATGTAGT+AGG	-	chr8_1:70073160-70073179	None:intergenic	30.0%
AGACAATTTACTTTATGCAG+GGG	-	chr8_1:70073348-70073367	None:intergenic	30.0%
GAGACAATTTACTTTATGCA+GGG	-	chr8_1:70073349-70073368	None:intergenic	30.0%
AGAGACAATTTACTTTATGC+AGG	-	chr8_1:70073350-70073369	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	70071713	70074661	70071713	ID=Msa1210850;Name=Msa1210850
chr8_1	mRNA	70071713	70074661	70071713	ID=Msa1210850-mRNA-1;Parent=Msa1210850;Name=Msa1210850-mRNA-1;_AED=0.11;_eAED=0.11;_QI=185\|1\|1\|1\|1\|1\|5\|179\|181
chr8_1	exon	70071713	70072035	70071713	ID=Msa1210850-mRNA-1:exon:23406;Parent=Msa1210850-mRNA-1
chr8_1	exon	70073047	70073143	70073047	ID=Msa1210850-mRNA-1:exon:23407;Parent=Msa1210850-mRNA-1
chr8_1	exon	70073262	70073302	70073262	ID=Msa1210850-mRNA-1:exon:23408;Parent=Msa1210850-mRNA-1
chr8_1	exon	70073393	70073482	70073393	ID=Msa1210850-mRNA-1:exon:23409;Parent=Msa1210850-mRNA-1
chr8_1	exon	70074303	70074661	70074303	ID=Msa1210850-mRNA-1:exon:23410;Parent=Msa1210850-mRNA-1
chr8_1	five_prime_UTR	70071713	70071897	70071713	ID=Msa1210850-mRNA-1:five_prime_utr;Parent=Msa1210850-mRNA-1
chr8_1	CDS	70071898	70072035	70071898	ID=Msa1210850-mRNA-1:cds;Parent=Msa1210850-mRNA-1
chr8_1	CDS	70073047	70073143	70073047	ID=Msa1210850-mRNA-1:cds;Parent=Msa1210850-mRNA-1
chr8_1	CDS	70073262	70073302	70073262	ID=Msa1210850-mRNA-1:cds;Parent=Msa1210850-mRNA-1
chr8_1	CDS	70073393	70073482	70073393	ID=Msa1210850-mRNA-1:cds;Parent=Msa1210850-mRNA-1
chr8_1	CDS	70074303	70074482	70074303	ID=Msa1210850-mRNA-1:cds;Parent=Msa1210850-mRNA-1
chr8_1	three_prime_UTR	70074483	70074661	70074483	ID=Msa1210850-mRNA-1:three_prime_utr;Parent=Msa1210850-mRNA-1

Gene Sequence

>Msa1210850

ATGGCATCATTTGCAGGCACTACACAGAAATGTAATACATGTGAAAAGAAAGTTTACTGGGTGGAACAGCTCACTGCTGATAACAAAGTGTACCACAAGTCTTGCTTCAGATGCCACCATTGCAAGGGCACACTCAAGCTGAGTAACTATTGTTCTTTTGAGGGTGTTCTATACTGTAAGCCTCATTTTGATCAACTCTTTAAGATGACTGGCAGTTTGGACAAAAGTTTTGAAGGTATTGCAAGAATTTATAGAGTTGAAAGATCAACTGATCAGGTCGAAGCATATAACAAGGTTTCAAGATTTTTTGCTGGAACTCAAGAAAAATGTGTTGGTTGCAAGAAAACAGTGTACCCAATTGAAAAGGTAGCTGTTGATGGAGAATCTTACCATAAGACTTGCTTCAGGTGCACACATGGAGGTTGCATAATCAGTCCATCAAACTATGTGGCACATGAACATCGCCTTTATTGTAGACATCACCATACTCAGATCTTCAAACAGAAGGGTAATTTCAGCCAATTTGATAAGGTTGAACAAGTCTAA

Protein sequence

>Msa1210850

MASFAGTTQKCNTCEKKVYWVEQLTADNKVYHKSCFRCHHCKGTLKLSNYCSFEGVLYCKPHFDQLFKMTGSLDKSFEGIARIYRVERSTDQVEAYNKVSRFFAGTQEKCVGCKKTVYPIEKVAVDGESYHKTCFRCTHGGCIISPSNYVAHEHRLYCRHHHTQIFKQKGNFSQFDKVEQV*