AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1213140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1213140 MtrunA17_Chr4g0061701 97.748 222 5 0 1 222 1 222 1.13e-162 447
Msa1213140 MtrunA17_Chr5g0420141 48.598 214 102 2 17 222 23 236 4.59e-66 204
Msa1213140 MtrunA17_Chr3g0114601 32.704 159 99 3 65 222 73 224 1.51e-23 94.0
Msa1213140 MtrunA17_Chr8g0380301 28.390 236 112 7 40 222 3 234 4.95e-19 82.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1213140 AT3G03290.1 51.163 172 80 2 55 222 103 274 1.11e-58 186
Msa1213140 AT5G17390.1 52.353 170 77 2 57 222 116 285 1.47e-57 184
Msa1213140 AT2G03720.2 47.087 206 98 7 26 222 29 232 7.18e-54 172
Msa1213140 AT1G69080.1 44.279 201 97 3 37 222 23 223 5.05e-51 165
Msa1213140 AT2G03720.1 50.602 166 77 4 61 222 1 165 2.19e-50 161
Msa1213140 AT1G69080.2 44.970 169 88 3 57 222 43 209 7.35e-43 144
Msa1213140 AT1G44760.1 37.805 164 88 4 59 222 64 213 1.85e-25 99.4
Msa1213140 AT1G44760.2 36.723 177 96 5 48 222 122 284 6.12e-25 99.4
Msa1213140 AT4G13450.1 29.808 208 98 7 59 222 16 219 1.32e-18 81.3

Find 54 sgRNAs with CRISPR-Local

Find 246 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATGTTCTGCATCTTCTTT+TGG 0.214542 8_1:+73224831 Msa1213140:CDS
TATGACTATATCAATGAAAT+TGG 0.241868 8_1:+73226295 Msa1213140:three_prime_UTR
GCAACAAAGGGTGTCTTTAT+TGG 0.266089 8_1:+73226075 Msa1213140:CDS
AAGCTAAAGGTGCTCTTGAT+TGG 0.333141 8_1:+73224970 Msa1213140:CDS
ACCCTTACTTTATGTGTATT+AGG 0.338897 8_1:-73226340 None:intergenic
ATACTCAGCAACCCCACTTT+TGG 0.340798 8_1:-73226161 None:intergenic
CACACATACTCTTCATATCT+AGG 0.373729 8_1:-73225235 None:intergenic
TGCCTAATACACATAAAGTA+AGG 0.393050 8_1:+73226338 Msa1213140:three_prime_UTR
ATTGCTTGAAGGGGAGGAAA+AGG 0.407520 8_1:+73226027 Msa1213140:CDS
AGCTAAAGGTGCTCTTGATT+GGG 0.425409 8_1:+73224971 Msa1213140:CDS
ACTTTGAACGAGCTTTCTTC+TGG 0.432468 8_1:+73226478 Msa1213140:three_prime_UTR
GAAATTGGTTATGGTGTTTG+TGG 0.432650 8_1:+73226310 Msa1213140:three_prime_UTR
AAGGGTGTCTTTATTGGTGG+TGG 0.462177 8_1:+73226081 Msa1213140:CDS
TATATCAATGAAATTGGTTA+TGG 0.465159 8_1:+73226301 Msa1213140:three_prime_UTR
GTGAATATAGTATTGCTTGA+AGG 0.468407 8_1:+73226016 Msa1213140:CDS
GAACAAGAACAACAGTATCT+TGG 0.472345 8_1:-73225016 None:intergenic
ATTCTTCCTCCTCACTGCAA+TGG 0.482834 8_1:-73226209 None:intergenic
AAATTGGTTATGGTGTTTGT+GGG 0.506424 8_1:+73226311 Msa1213140:three_prime_UTR
TGAATATAGTATTGCTTGAA+GGG 0.506592 8_1:+73226017 Msa1213140:CDS
ACTCTGGAAGAGATAACAAA+GGG 0.515139 8_1:+73224901 Msa1213140:CDS
GATTCTAGCTTTGAAGCTAA+AGG 0.523893 8_1:+73224957 Msa1213140:CDS
TATAGTATTGCTTGAAGGGG+AGG 0.536510 8_1:+73226021 Msa1213140:CDS
CTCACTGCAATGGTCATACA+AGG 0.537662 8_1:-73226199 None:intergenic
TGCAAGAGGCAAAGCAACAA+AGG 0.539284 8_1:+73226062 Msa1213140:CDS
GCAAGAGGCAAAGCAACAAA+GGG 0.539516 8_1:+73226063 Msa1213140:CDS
ACAAAGGGTGTCTTTATTGG+TGG 0.547210 8_1:+73226078 Msa1213140:CDS
AGCAACGGAGTTCAAGACTC+TGG 0.548078 8_1:+73224885 Msa1213140:CDS
CAATAAAGTGATGGTTGTTG+TGG 0.548385 8_1:+73224935 Msa1213140:CDS
TGTGGTGCTTGATGAGGAAG+TGG 0.561004 8_1:+73226125 Msa1213140:CDS
TTGCTTGAAGGGGAGGAAAA+GGG 0.567097 8_1:+73226028 Msa1213140:CDS
GATAACAAAGGGGATGAAAG+TGG 0.578843 8_1:+73224912 Msa1213140:CDS
TGATGTGAAGTTTAATCTGA+AGG 0.584331 8_1:+73225199 Msa1213140:CDS
GGGTGCTGCAATAGTGCAAG+AGG 0.588176 8_1:+73226048 Msa1213140:CDS
GAATCAATTAATCAAAGACA+TGG 0.589079 8_1:+73224746 Msa1213140:exon
AGGGTGTCTTTATTGGTGGT+GGG 0.591794 8_1:+73226082 Msa1213140:CDS
AGAAAGCTCGTTCAAAGTGA+AGG 0.595325 8_1:-73226473 None:intergenic
ATGTGTGAAATGAAGAAACC+TGG 0.596732 8_1:+73225251 Msa1213140:CDS
CAAGAAAGAGAACCAAAAGT+GGG 0.599249 8_1:+73226149 Msa1213140:CDS
ACAAGAAAGAGAACCAAAAG+TGG 0.608229 8_1:+73226148 Msa1213140:CDS
GAATATAGTATTGCTTGAAG+GGG 0.613739 8_1:+73226018 Msa1213140:CDS
AAGTTGCATAGTTTGTGCGT+TGG 0.613774 8_1:+73224783 Msa1213140:CDS
GCCTAATACACATAAAGTAA+GGG 0.626152 8_1:+73226339 Msa1213140:three_prime_UTR
GACTCTGGAAGAGATAACAA+AGG 0.639056 8_1:+73224900 Msa1213140:CDS
TGTGTGAAATGAAGAAACCT+GGG 0.642180 8_1:+73225252 Msa1213140:CDS
GTATGACCATTGCAGTGAGG+AGG 0.646086 8_1:+73226203 Msa1213140:CDS
GTAGCTAGACCCCTCAGAGG+TGG 0.650823 8_1:+73225041 Msa1213140:CDS
GCAGAACATGCTCGAACACG+AGG 0.664452 8_1:-73224819 None:intergenic
TGAAAGTGGCAATAAAGTGA+TGG 0.668312 8_1:+73224926 Msa1213140:CDS
AAACTATGCAACTTGAAACG+TGG 0.681932 8_1:-73224774 None:intergenic
CATGTAGCTAGACCCCTCAG+AGG 0.708670 8_1:+73225038 Msa1213140:CDS
CTTGTATGACCATTGCAGTG+AGG 0.716302 8_1:+73226200 Msa1213140:CDS
AAGAAAGAGAACCAAAAGTG+GGG 0.725592 8_1:+73226150 Msa1213140:CDS
CTCTGGAAGAGATAACAAAG+GGG 0.742102 8_1:+73224902 Msa1213140:CDS
GTGTGAAATGAAGAAACCTG+GGG 0.759265 8_1:+73225253 Msa1213140:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTAAAATACATTTTAAAATT+TGG - chr8_1:73225681-73225700 None:intergenic 10.0%
!!! CTAAAATACATTTTAAAATT+TGG - chr8_1:73225681-73225700 None:intergenic 10.0%
!!! TTTCTTTCTAAAATTGTAAT+TGG + chr8_1:73225107-73225126 Msa1213140:intron 15.0%
!! TAATTAGAGTTATATTGTAT+TGG - chr8_1:73225446-73225465 None:intergenic 15.0%
!!! AAATGTATTTTAGATTTGTT+TGG + chr8_1:73225688-73225707 Msa1213140:intron 15.0%
!! TTCTCATTTATTTAATCAAA+TGG + chr8_1:73225750-73225769 Msa1213140:intron 15.0%
!! ATTACTAATAGCATAAATTA+TGG - chr8_1:73225853-73225872 None:intergenic 15.0%
!!! TTTCTTTCTAAAATTGTAAT+TGG + chr8_1:73225107-73225126 Msa1213140:intron 15.0%
!! TAATTAGAGTTATATTGTAT+TGG - chr8_1:73225446-73225465 None:intergenic 15.0%
!!! AAATGTATTTTAGATTTGTT+TGG + chr8_1:73225688-73225707 Msa1213140:intron 15.0%
!! TTCTCATTTATTTAATCAAA+TGG + chr8_1:73225750-73225769 Msa1213140:intron 15.0%
!! ATTACTAATAGCATAAATTA+TGG - chr8_1:73225853-73225872 None:intergenic 15.0%
!! TCAACAAAAGTTAATGTATT+TGG + chr8_1:73225503-73225522 Msa1213140:intron 20.0%
!! AATGTATTTGGTTAAAAACT+TGG + chr8_1:73225515-73225534 Msa1213140:intron 20.0%
!!! ATAAAACTGACTTTTAAATG+AGG - chr8_1:73225636-73225655 None:intergenic 20.0%
!! TATGACTATATCAATGAAAT+TGG + chr8_1:73226295-73226314 Msa1213140:three_prime_UTR 20.0%
!! TATATCAATGAAATTGGTTA+TGG + chr8_1:73226301-73226320 Msa1213140:three_prime_UTR 20.0%
!! TCAACAAAAGTTAATGTATT+TGG + chr8_1:73225503-73225522 Msa1213140:intron 20.0%
!! AATGTATTTGGTTAAAAACT+TGG + chr8_1:73225515-73225534 Msa1213140:intron 20.0%
!!! ATAAAACTGACTTTTAAATG+AGG - chr8_1:73225636-73225655 None:intergenic 20.0%
!! TATGACTATATCAATGAAAT+TGG + chr8_1:73226295-73226314 Msa1213140:three_prime_UTR 20.0%
!! TATATCAATGAAATTGGTTA+TGG + chr8_1:73226301-73226320 Msa1213140:three_prime_UTR 20.0%
! GAATCAATTAATCAAAGACA+TGG + chr8_1:73224746-73224765 Msa1213140:exon 25.0%
! TAATTGGACTAATGTGAAAA+GGG + chr8_1:73225123-73225142 Msa1213140:intron 25.0%
!!! TGTTCTATTGATTCATTACT+AGG + chr8_1:73225167-73225186 Msa1213140:intron 25.0%
!! TGTTACTTGATATTGTGTTA+AGG + chr8_1:73225310-73225329 Msa1213140:intron 25.0%
! GTTACTTGATATTGTGTTAA+GGG + chr8_1:73225311-73225330 Msa1213140:intron 25.0%
!!! GGACACTTTTTTTAGTAAAT+GGG - chr8_1:73225377-73225396 None:intergenic 25.0%
!!! TGAAATGACTCAGTTATTTT+GGG - chr8_1:73225398-73225417 None:intergenic 25.0%
!! TTGAAATGACTCAGTTATTT+TGG - chr8_1:73225399-73225418 None:intergenic 25.0%
! CCAAAATTGCTCTTATAAAT+AGG - chr8_1:73225483-73225502 None:intergenic 25.0%
!! CCTATTTATAAGAGCAATTT+TGG + chr8_1:73225480-73225499 Msa1213140:intron 25.0%
! TCAACAAAAGTGAATGTATT+TGG - chr8_1:73225542-73225561 None:intergenic 25.0%
! TTTGGGTATAAGTTTATAAG+AGG + chr8_1:73225603-73225622 Msa1213140:intron 25.0%
! TGAATATAGTATTGCTTGAA+GGG + chr8_1:73226017-73226036 Msa1213140:CDS 25.0%
!! AAAGAAAACGTTCTATTTTG+TGG + chr8_1:73226107-73226126 Msa1213140:CDS 25.0%
! GGACAAAAATATTCAAGTAA+GGG - chr8_1:73226455-73226474 None:intergenic 25.0%
! AGGACAAAAATATTCAAGTA+AGG - chr8_1:73226456-73226475 None:intergenic 25.0%
!! AAATGTCGTGTATATTTTCT+AGG - chr8_1:73226519-73226538 None:intergenic 25.0%
! GAATCAATTAATCAAAGACA+TGG + chr8_1:73224746-73224765 Msa1213140:exon 25.0%
! TAATTGGACTAATGTGAAAA+GGG + chr8_1:73225123-73225142 Msa1213140:intron 25.0%
!!! TGTTCTATTGATTCATTACT+AGG + chr8_1:73225167-73225186 Msa1213140:intron 25.0%
!! TGTTACTTGATATTGTGTTA+AGG + chr8_1:73225310-73225329 Msa1213140:intron 25.0%
! GTTACTTGATATTGTGTTAA+GGG + chr8_1:73225311-73225330 Msa1213140:intron 25.0%
!!! GGACACTTTTTTTAGTAAAT+GGG - chr8_1:73225377-73225396 None:intergenic 25.0%
!!! TGAAATGACTCAGTTATTTT+GGG - chr8_1:73225398-73225417 None:intergenic 25.0%
!! TTGAAATGACTCAGTTATTT+TGG - chr8_1:73225399-73225418 None:intergenic 25.0%
! CCAAAATTGCTCTTATAAAT+AGG - chr8_1:73225483-73225502 None:intergenic 25.0%
!! CCTATTTATAAGAGCAATTT+TGG + chr8_1:73225480-73225499 Msa1213140:intron 25.0%
! TCAACAAAAGTGAATGTATT+TGG - chr8_1:73225542-73225561 None:intergenic 25.0%
! TTTGGGTATAAGTTTATAAG+AGG + chr8_1:73225603-73225622 Msa1213140:intron 25.0%
! TGAATATAGTATTGCTTGAA+GGG + chr8_1:73226017-73226036 Msa1213140:CDS 25.0%
!! AAAGAAAACGTTCTATTTTG+TGG + chr8_1:73226107-73226126 Msa1213140:CDS 25.0%
! GGACAAAAATATTCAAGTAA+GGG - chr8_1:73226455-73226474 None:intergenic 25.0%
! AGGACAAAAATATTCAAGTA+AGG - chr8_1:73226456-73226475 None:intergenic 25.0%
!! AAATGTCGTGTATATTTTCT+AGG - chr8_1:73226519-73226538 None:intergenic 25.0%
GTAATTGGACTAATGTGAAA+AGG + chr8_1:73225122-73225141 Msa1213140:intron 30.0%
AATTGGACTAATGTGAAAAG+GGG + chr8_1:73225124-73225143 Msa1213140:intron 30.0%
TGATGTGAAGTTTAATCTGA+AGG + chr8_1:73225199-73225218 Msa1213140:CDS 30.0%
!!! GGGACACTTTTTTTAGTAAA+TGG - chr8_1:73225378-73225397 None:intergenic 30.0%
ACTCTAATTAATACTACCTC+CGG + chr8_1:73225456-73225475 Msa1213140:intron 30.0%
ATTGCTCTTATAAATAGGAC+CGG - chr8_1:73225478-73225497 None:intergenic 30.0%
TATAAGTTTATAAGAGGAGG+CGG + chr8_1:73225609-73225628 Msa1213140:intron 30.0%
GTGAATATAGTATTGCTTGA+AGG + chr8_1:73226016-73226035 Msa1213140:CDS 30.0%
GAATATAGTATTGCTTGAAG+GGG + chr8_1:73226018-73226037 Msa1213140:CDS 30.0%
AGGAGGAAGAATAAAAAACT+TGG + chr8_1:73226220-73226239 Msa1213140:CDS 30.0%
AGGAAGAATAAAAAACTTGG+AGG + chr8_1:73226223-73226242 Msa1213140:CDS 30.0%
GGAAGAATAAAAAACTTGGA+GGG + chr8_1:73226224-73226243 Msa1213140:CDS 30.0%
! CCAAAAGCCAAAAGTTTTTA+TGG - chr8_1:73226270-73226289 None:intergenic 30.0%
!!! CCATAAAAACTTTTGGCTTT+TGG + chr8_1:73226267-73226286 Msa1213140:CDS 30.0%
AAATTGGTTATGGTGTTTGT+GGG + chr8_1:73226311-73226330 Msa1213140:three_prime_UTR 30.0%
TGCCTAATACACATAAAGTA+AGG + chr8_1:73226338-73226357 Msa1213140:three_prime_UTR 30.0%
GCCTAATACACATAAAGTAA+GGG + chr8_1:73226339-73226358 Msa1213140:three_prime_UTR 30.0%
ACCCTTACTTTATGTGTATT+AGG - chr8_1:73226343-73226362 None:intergenic 30.0%
GTAATTGGACTAATGTGAAA+AGG + chr8_1:73225122-73225141 Msa1213140:intron 30.0%
AATTGGACTAATGTGAAAAG+GGG + chr8_1:73225124-73225143 Msa1213140:intron 30.0%
TGATGTGAAGTTTAATCTGA+AGG + chr8_1:73225199-73225218 Msa1213140:CDS 30.0%
!!! GGGACACTTTTTTTAGTAAA+TGG - chr8_1:73225378-73225397 None:intergenic 30.0%
ACTCTAATTAATACTACCTC+CGG + chr8_1:73225456-73225475 Msa1213140:intron 30.0%
ATTGCTCTTATAAATAGGAC+CGG - chr8_1:73225478-73225497 None:intergenic 30.0%
TATAAGTTTATAAGAGGAGG+CGG + chr8_1:73225609-73225628 Msa1213140:intron 30.0%
GTGAATATAGTATTGCTTGA+AGG + chr8_1:73226016-73226035 Msa1213140:CDS 30.0%
GAATATAGTATTGCTTGAAG+GGG + chr8_1:73226018-73226037 Msa1213140:CDS 30.0%
AGGAGGAAGAATAAAAAACT+TGG + chr8_1:73226220-73226239 Msa1213140:CDS 30.0%
AGGAAGAATAAAAAACTTGG+AGG + chr8_1:73226223-73226242 Msa1213140:CDS 30.0%
GGAAGAATAAAAAACTTGGA+GGG + chr8_1:73226224-73226243 Msa1213140:CDS 30.0%
! CCAAAAGCCAAAAGTTTTTA+TGG - chr8_1:73226270-73226289 None:intergenic 30.0%
!!! CCATAAAAACTTTTGGCTTT+TGG + chr8_1:73226267-73226286 Msa1213140:CDS 30.0%
AAATTGGTTATGGTGTTTGT+GGG + chr8_1:73226311-73226330 Msa1213140:three_prime_UTR 30.0%
TGCCTAATACACATAAAGTA+AGG + chr8_1:73226338-73226357 Msa1213140:three_prime_UTR 30.0%
GCCTAATACACATAAAGTAA+GGG + chr8_1:73226339-73226358 Msa1213140:three_prime_UTR 30.0%
ACCCTTACTTTATGTGTATT+AGG - chr8_1:73226343-73226362 None:intergenic 30.0%
AAACTATGCAACTTGAAACG+TGG - chr8_1:73224777-73224796 None:intergenic 35.0%
!! AGAACTGAGTTTTCAAGCAA+CGG + chr8_1:73224870-73224889 Msa1213140:CDS 35.0%
! ACTCTGGAAGAGATAACAAA+GGG + chr8_1:73224901-73224920 Msa1213140:CDS 35.0%
TGAAAGTGGCAATAAAGTGA+TGG + chr8_1:73224926-73224945 Msa1213140:CDS 35.0%
CAATAAAGTGATGGTTGTTG+TGG + chr8_1:73224935-73224954 Msa1213140:CDS 35.0%
!! GATTCTAGCTTTGAAGCTAA+AGG + chr8_1:73224957-73224976 Msa1213140:CDS 35.0%
GAACAAGAACAACAGTATCT+TGG - chr8_1:73225019-73225038 None:intergenic 35.0%
CACACATACTCTTCATATCT+AGG - chr8_1:73225238-73225257 None:intergenic 35.0%
ATGTGTGAAATGAAGAAACC+TGG + chr8_1:73225251-73225270 Msa1213140:CDS 35.0%
TGTGTGAAATGAAGAAACCT+GGG + chr8_1:73225252-73225271 Msa1213140:CDS 35.0%
GGGTATAAGTTTATAAGAGG+AGG + chr8_1:73225606-73225625 Msa1213140:intron 35.0%
TATGCACACACATATGCATA+TGG + chr8_1:73225961-73225980 Msa1213140:intron 35.0%
CATATGCATATGGTAACATG+TGG + chr8_1:73225971-73225990 Msa1213140:intron 35.0%
ATGGTAACATGTGGAGTTTA+TGG + chr8_1:73225980-73225999 Msa1213140:intron 35.0%
TGGTAACATGTGGAGTTTAT+GGG + chr8_1:73225981-73226000 Msa1213140:intron 35.0%
ACAAGAAAGAGAACCAAAAG+TGG + chr8_1:73226148-73226167 Msa1213140:CDS 35.0%
CAAGAAAGAGAACCAAAAGT+GGG + chr8_1:73226149-73226168 Msa1213140:CDS 35.0%
AAGAAAGAGAACCAAAAGTG+GGG + chr8_1:73226150-73226169 Msa1213140:CDS 35.0%
! CCAAAAGTTTTTATGGCGTT+TGG - chr8_1:73226263-73226282 None:intergenic 35.0%
! CCAAACGCCATAAAAACTTT+TGG + chr8_1:73226260-73226279 Msa1213140:CDS 35.0%
GAAATTGGTTATGGTGTTTG+TGG + chr8_1:73226310-73226329 Msa1213140:three_prime_UTR 35.0%
AAACTATGCAACTTGAAACG+TGG - chr8_1:73224777-73224796 None:intergenic 35.0%
!! AGAACTGAGTTTTCAAGCAA+CGG + chr8_1:73224870-73224889 Msa1213140:CDS 35.0%
! ACTCTGGAAGAGATAACAAA+GGG + chr8_1:73224901-73224920 Msa1213140:CDS 35.0%
TGAAAGTGGCAATAAAGTGA+TGG + chr8_1:73224926-73224945 Msa1213140:CDS 35.0%
CAATAAAGTGATGGTTGTTG+TGG + chr8_1:73224935-73224954 Msa1213140:CDS 35.0%
!! GATTCTAGCTTTGAAGCTAA+AGG + chr8_1:73224957-73224976 Msa1213140:CDS 35.0%
GAACAAGAACAACAGTATCT+TGG - chr8_1:73225019-73225038 None:intergenic 35.0%
CACACATACTCTTCATATCT+AGG - chr8_1:73225238-73225257 None:intergenic 35.0%
ATGTGTGAAATGAAGAAACC+TGG + chr8_1:73225251-73225270 Msa1213140:CDS 35.0%
TGTGTGAAATGAAGAAACCT+GGG + chr8_1:73225252-73225271 Msa1213140:CDS 35.0%
GGGTATAAGTTTATAAGAGG+AGG + chr8_1:73225606-73225625 Msa1213140:intron 35.0%
TATGCACACACATATGCATA+TGG + chr8_1:73225961-73225980 Msa1213140:intron 35.0%
CATATGCATATGGTAACATG+TGG + chr8_1:73225971-73225990 Msa1213140:intron 35.0%
ATGGTAACATGTGGAGTTTA+TGG + chr8_1:73225980-73225999 Msa1213140:intron 35.0%
TGGTAACATGTGGAGTTTAT+GGG + chr8_1:73225981-73226000 Msa1213140:intron 35.0%
ACAAGAAAGAGAACCAAAAG+TGG + chr8_1:73226148-73226167 Msa1213140:CDS 35.0%
CAAGAAAGAGAACCAAAAGT+GGG + chr8_1:73226149-73226168 Msa1213140:CDS 35.0%
AAGAAAGAGAACCAAAAGTG+GGG + chr8_1:73226150-73226169 Msa1213140:CDS 35.0%
! CCAAAAGTTTTTATGGCGTT+TGG - chr8_1:73226263-73226282 None:intergenic 35.0%
! CCAAACGCCATAAAAACTTT+TGG + chr8_1:73226260-73226279 Msa1213140:CDS 35.0%
GAAATTGGTTATGGTGTTTG+TGG + chr8_1:73226310-73226329 Msa1213140:three_prime_UTR 35.0%
! AAGTTGCATAGTTTGTGCGT+TGG + chr8_1:73224783-73224802 Msa1213140:CDS 40.0%
! GCATGTTCTGCATCTTCTTT+TGG + chr8_1:73224831-73224850 Msa1213140:CDS 40.0%
! GACTCTGGAAGAGATAACAA+AGG + chr8_1:73224900-73224919 Msa1213140:CDS 40.0%
! CTCTGGAAGAGATAACAAAG+GGG + chr8_1:73224902-73224921 Msa1213140:CDS 40.0%
GATAACAAAGGGGATGAAAG+TGG + chr8_1:73224912-73224931 Msa1213140:CDS 40.0%
!! AAGCTAAAGGTGCTCTTGAT+TGG + chr8_1:73224970-73224989 Msa1213140:CDS 40.0%
!! AGCTAAAGGTGCTCTTGATT+GGG + chr8_1:73224971-73224990 Msa1213140:CDS 40.0%
!!! TTTTTTCTGACCACCTCTGA+GGG - chr8_1:73225054-73225073 None:intergenic 40.0%
GTGTGAAATGAAGAAACCTG+GGG + chr8_1:73225253-73225272 Msa1213140:CDS 40.0%
CTTGTTTGAAAACTTACCCC+AGG - chr8_1:73225272-73225291 None:intergenic 40.0%
CGCAACTAACTCAGATATAC+CGG + chr8_1:73225562-73225581 Msa1213140:intron 40.0%
GCAACTAACTCAGATATACC+GGG + chr8_1:73225563-73225582 Msa1213140:intron 40.0%
! ACGGAGGGAGTATATAGTTT+GGG + chr8_1:73225586-73225605 Msa1213140:intron 40.0%
TCAAATGGTTCGACTCATCA+CGG + chr8_1:73225765-73225784 Msa1213140:intron 40.0%
ATGTGGAGTTTATGGGTTTC+AGG + chr8_1:73225988-73226007 Msa1213140:intron 40.0%
TATAGTATTGCTTGAAGGGG+AGG + chr8_1:73226021-73226040 Msa1213140:CDS 40.0%
!! GCAACAAAGGGTGTCTTTAT+TGG + chr8_1:73226075-73226094 Msa1213140:CDS 40.0%
!! ACAAAGGGTGTCTTTATTGG+TGG + chr8_1:73226078-73226097 Msa1213140:CDS 40.0%
! CTATTTTGTGGTGCTTGATG+AGG + chr8_1:73226119-73226138 Msa1213140:CDS 40.0%
AGAAAGCTCGTTCAAAGTGA+AGG - chr8_1:73226476-73226495 None:intergenic 40.0%
ACTTTGAACGAGCTTTCTTC+TGG + chr8_1:73226478-73226497 Msa1213140:three_prime_UTR 40.0%
!! CGTGTATATTTTCTAGGAGC+AGG - chr8_1:73226513-73226532 None:intergenic 40.0%
! AAGTTGCATAGTTTGTGCGT+TGG + chr8_1:73224783-73224802 Msa1213140:CDS 40.0%
! GCATGTTCTGCATCTTCTTT+TGG + chr8_1:73224831-73224850 Msa1213140:CDS 40.0%
! GACTCTGGAAGAGATAACAA+AGG + chr8_1:73224900-73224919 Msa1213140:CDS 40.0%
! CTCTGGAAGAGATAACAAAG+GGG + chr8_1:73224902-73224921 Msa1213140:CDS 40.0%
GATAACAAAGGGGATGAAAG+TGG + chr8_1:73224912-73224931 Msa1213140:CDS 40.0%
!! AAGCTAAAGGTGCTCTTGAT+TGG + chr8_1:73224970-73224989 Msa1213140:CDS 40.0%
!! AGCTAAAGGTGCTCTTGATT+GGG + chr8_1:73224971-73224990 Msa1213140:CDS 40.0%
!!! TTTTTTCTGACCACCTCTGA+GGG - chr8_1:73225054-73225073 None:intergenic 40.0%
GTGTGAAATGAAGAAACCTG+GGG + chr8_1:73225253-73225272 Msa1213140:CDS 40.0%
CTTGTTTGAAAACTTACCCC+AGG - chr8_1:73225272-73225291 None:intergenic 40.0%
CGCAACTAACTCAGATATAC+CGG + chr8_1:73225562-73225581 Msa1213140:intron 40.0%
GCAACTAACTCAGATATACC+GGG + chr8_1:73225563-73225582 Msa1213140:intron 40.0%
! ACGGAGGGAGTATATAGTTT+GGG + chr8_1:73225586-73225605 Msa1213140:intron 40.0%
TCAAATGGTTCGACTCATCA+CGG + chr8_1:73225765-73225784 Msa1213140:intron 40.0%
ATGTGGAGTTTATGGGTTTC+AGG + chr8_1:73225988-73226007 Msa1213140:intron 40.0%
TATAGTATTGCTTGAAGGGG+AGG + chr8_1:73226021-73226040 Msa1213140:CDS 40.0%
!! GCAACAAAGGGTGTCTTTAT+TGG + chr8_1:73226075-73226094 Msa1213140:CDS 40.0%
!! ACAAAGGGTGTCTTTATTGG+TGG + chr8_1:73226078-73226097 Msa1213140:CDS 40.0%
! CTATTTTGTGGTGCTTGATG+AGG + chr8_1:73226119-73226138 Msa1213140:CDS 40.0%
AGAAAGCTCGTTCAAAGTGA+AGG - chr8_1:73226476-73226495 None:intergenic 40.0%
ACTTTGAACGAGCTTTCTTC+TGG + chr8_1:73226478-73226497 Msa1213140:three_prime_UTR 40.0%
!! CGTGTATATTTTCTAGGAGC+AGG - chr8_1:73226513-73226532 None:intergenic 40.0%
! TTTTTCTGACCACCTCTGAG+GGG - chr8_1:73225053-73225072 None:intergenic 45.0%
!!! CTTTTTTCTGACCACCTCTG+AGG - chr8_1:73225055-73225074 None:intergenic 45.0%
AAATGATGCTACCTCCGTCT+CGG + chr8_1:73225342-73225361 Msa1213140:intron 45.0%
ATGGGTCAGATATTCCGAGA+CGG - chr8_1:73225359-73225378 None:intergenic 45.0%
GCTCTTATAAATAGGACCGG+AGG - chr8_1:73225475-73225494 None:intergenic 45.0%
CTAACTCAGATATACCGGGA+CGG + chr8_1:73225567-73225586 Msa1213140:intron 45.0%
! GACGGAGGGAGTATATAGTT+TGG + chr8_1:73225585-73225604 Msa1213140:intron 45.0%
! AGTTTATGGGTTTCAGGTGC+TGG + chr8_1:73225994-73226013 Msa1213140:intron 45.0%
ATTGCTTGAAGGGGAGGAAA+AGG + chr8_1:73226027-73226046 Msa1213140:CDS 45.0%
TTGCTTGAAGGGGAGGAAAA+GGG + chr8_1:73226028-73226047 Msa1213140:CDS 45.0%
TGCAAGAGGCAAAGCAACAA+AGG + chr8_1:73226062-73226081 Msa1213140:CDS 45.0%
GCAAGAGGCAAAGCAACAAA+GGG + chr8_1:73226063-73226082 Msa1213140:CDS 45.0%
!! AAGGGTGTCTTTATTGGTGG+TGG + chr8_1:73226081-73226100 Msa1213140:CDS 45.0%
! AGGGTGTCTTTATTGGTGGT+GGG + chr8_1:73226082-73226101 Msa1213140:CDS 45.0%
! ATACTCAGCAACCCCACTTT+TGG - chr8_1:73226164-73226183 None:intergenic 45.0%
CTCACTGCAATGGTCATACA+AGG - chr8_1:73226202-73226221 None:intergenic 45.0%
CTTGTATGACCATTGCAGTG+AGG + chr8_1:73226200-73226219 Msa1213140:CDS 45.0%
ATTCTTCCTCCTCACTGCAA+TGG - chr8_1:73226212-73226231 None:intergenic 45.0%
! TTTTTCTGACCACCTCTGAG+GGG - chr8_1:73225053-73225072 None:intergenic 45.0%
!!! CTTTTTTCTGACCACCTCTG+AGG - chr8_1:73225055-73225074 None:intergenic 45.0%
AAATGATGCTACCTCCGTCT+CGG + chr8_1:73225342-73225361 Msa1213140:intron 45.0%
ATGGGTCAGATATTCCGAGA+CGG - chr8_1:73225359-73225378 None:intergenic 45.0%
GCTCTTATAAATAGGACCGG+AGG - chr8_1:73225475-73225494 None:intergenic 45.0%
CTAACTCAGATATACCGGGA+CGG + chr8_1:73225567-73225586 Msa1213140:intron 45.0%
! GACGGAGGGAGTATATAGTT+TGG + chr8_1:73225585-73225604 Msa1213140:intron 45.0%
! AGTTTATGGGTTTCAGGTGC+TGG + chr8_1:73225994-73226013 Msa1213140:intron 45.0%
ATTGCTTGAAGGGGAGGAAA+AGG + chr8_1:73226027-73226046 Msa1213140:CDS 45.0%
TTGCTTGAAGGGGAGGAAAA+GGG + chr8_1:73226028-73226047 Msa1213140:CDS 45.0%
TGCAAGAGGCAAAGCAACAA+AGG + chr8_1:73226062-73226081 Msa1213140:CDS 45.0%
GCAAGAGGCAAAGCAACAAA+GGG + chr8_1:73226063-73226082 Msa1213140:CDS 45.0%
!! AAGGGTGTCTTTATTGGTGG+TGG + chr8_1:73226081-73226100 Msa1213140:CDS 45.0%
! AGGGTGTCTTTATTGGTGGT+GGG + chr8_1:73226082-73226101 Msa1213140:CDS 45.0%
! ATACTCAGCAACCCCACTTT+TGG - chr8_1:73226164-73226183 None:intergenic 45.0%
CTCACTGCAATGGTCATACA+AGG - chr8_1:73226202-73226221 None:intergenic 45.0%
CTTGTATGACCATTGCAGTG+AGG + chr8_1:73226200-73226219 Msa1213140:CDS 45.0%
ATTCTTCCTCCTCACTGCAA+TGG - chr8_1:73226212-73226231 None:intergenic 45.0%
!!! TAAAATACATTTTAAAATTT+GGG - chr8_1:73225680-73225699 None:intergenic 5.0%
!!! TAATTATTTTAATTAATCTA+TGG - chr8_1:73225900-73225919 None:intergenic 5.0%
!!! TAAAATACATTTTAAAATTT+GGG - chr8_1:73225680-73225699 None:intergenic 5.0%
!!! TAATTATTTTAATTAATCTA+TGG - chr8_1:73225900-73225919 None:intergenic 5.0%
AGCAACGGAGTTCAAGACTC+TGG + chr8_1:73224885-73224904 Msa1213140:CDS 50.0%
ACTATATACTCCCTCCGTCC+CGG - chr8_1:73225584-73225603 None:intergenic 50.0%
GAGAGCGGTGACTCAATAGT+AGG - chr8_1:73225719-73225738 None:intergenic 50.0%
AGAAGTCTTTGCCTCGAGAG+CGG - chr8_1:73225734-73225753 None:intergenic 50.0%
TGTGGTGCTTGATGAGGAAG+TGG + chr8_1:73226125-73226144 Msa1213140:CDS 50.0%
GTATGACCATTGCAGTGAGG+AGG + chr8_1:73226203-73226222 Msa1213140:CDS 50.0%
AGCAACGGAGTTCAAGACTC+TGG + chr8_1:73224885-73224904 Msa1213140:CDS 50.0%
ACTATATACTCCCTCCGTCC+CGG - chr8_1:73225584-73225603 None:intergenic 50.0%
GAGAGCGGTGACTCAATAGT+AGG - chr8_1:73225719-73225738 None:intergenic 50.0%
AGAAGTCTTTGCCTCGAGAG+CGG - chr8_1:73225734-73225753 None:intergenic 50.0%
TGTGGTGCTTGATGAGGAAG+TGG + chr8_1:73226125-73226144 Msa1213140:CDS 50.0%
GTATGACCATTGCAGTGAGG+AGG + chr8_1:73226203-73226222 Msa1213140:CDS 50.0%
GCAGAACATGCTCGAACACG+AGG - chr8_1:73224822-73224841 None:intergenic 55.0%
CATGTAGCTAGACCCCTCAG+AGG + chr8_1:73225038-73225057 Msa1213140:CDS 55.0%
GGTCAGATATTCCGAGACGG+AGG - chr8_1:73225356-73225375 None:intergenic 55.0%
ACTCAGATATACCGGGACGG+AGG + chr8_1:73225570-73225589 Msa1213140:intron 55.0%
CTCAGATATACCGGGACGGA+GGG + chr8_1:73225571-73225590 Msa1213140:intron 55.0%
CTATTGAGTCACCGCTCTCG+AGG + chr8_1:73225720-73225739 Msa1213140:intron 55.0%
! GGGTGCTGCAATAGTGCAAG+AGG + chr8_1:73226048-73226067 Msa1213140:CDS 55.0%
GCAGAACATGCTCGAACACG+AGG - chr8_1:73224822-73224841 None:intergenic 55.0%
CATGTAGCTAGACCCCTCAG+AGG + chr8_1:73225038-73225057 Msa1213140:CDS 55.0%
GGTCAGATATTCCGAGACGG+AGG - chr8_1:73225356-73225375 None:intergenic 55.0%
ACTCAGATATACCGGGACGG+AGG + chr8_1:73225570-73225589 Msa1213140:intron 55.0%
CTCAGATATACCGGGACGGA+GGG + chr8_1:73225571-73225590 Msa1213140:intron 55.0%
CTATTGAGTCACCGCTCTCG+AGG + chr8_1:73225720-73225739 Msa1213140:intron 55.0%
! GGGTGCTGCAATAGTGCAAG+AGG + chr8_1:73226048-73226067 Msa1213140:CDS 55.0%
GTAGCTAGACCCCTCAGAGG+TGG + chr8_1:73225041-73225060 Msa1213140:CDS 60.0%
GTAGCTAGACCCCTCAGAGG+TGG + chr8_1:73225041-73225060 Msa1213140:CDS 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 73224730 73226539 73224730 ID=Msa1213140;Name=Msa1213140
chr8_1 mRNA 73224730 73226539 73224730 ID=Msa1213140-mRNA-1;Parent=Msa1213140;Name=Msa1213140-mRNA-1;_AED=0.07;_eAED=0.07;_QI=35|1|1|1|1|1|3|245|222
chr8_1 exon 73224730 73225062 73224730 ID=Msa1213140-mRNA-1:exon:24850;Parent=Msa1213140-mRNA-1
chr8_1 exon 73225189 73225274 73225189 ID=Msa1213140-mRNA-1:exon:24851;Parent=Msa1213140-mRNA-1
chr8_1 exon 73226010 73226539 73226010 ID=Msa1213140-mRNA-1:exon:24852;Parent=Msa1213140-mRNA-1
chr8_1 five_prime_UTR 73224730 73224764 73224730 ID=Msa1213140-mRNA-1:five_prime_utr;Parent=Msa1213140-mRNA-1
chr8_1 CDS 73224765 73225062 73224765 ID=Msa1213140-mRNA-1:cds;Parent=Msa1213140-mRNA-1
chr8_1 CDS 73225189 73225274 73225189 ID=Msa1213140-mRNA-1:cds;Parent=Msa1213140-mRNA-1
chr8_1 CDS 73226010 73226294 73226010 ID=Msa1213140-mRNA-1:cds;Parent=Msa1213140-mRNA-1
chr8_1 three_prime_UTR 73226295 73226539 73226295 ID=Msa1213140-mRNA-1:three_prime_utr;Parent=Msa1213140-mRNA-1
Gene Sequence

>Msa1213140

ATGGTTAGACCACGTTTCAAGTTGCATAGTTTGTGCGTTGGTCATGCTAGAACACCTCGTGTTCGAGCATGTTCTGCATCTTCTTTTGGATCAAAATTTGCAAACAGAACTGAGTTTTCAAGCAACGGAGTTCAAGACTCTGGAAGAGATAACAAAGGGGATGAAAGTGGCAATAAAGTGATGGTTGTTGTGGATTCTAGCTTTGAAGCTAAAGGTGCTCTTGATTGGGCACTCTCACACACTATTCAGAGCCAAGATACTGTTGTTCTTGTTCATGTAGCTAGACCCCTCAGAGGTGGCGACGAGTCTGATGTGAAGTTTAATCTGAAGGCATATCAACTTCTCCTAGATATGAAGAGTATGTGTGAAATGAAGAAACCTGGGGTGCTGGTGAATATAGTATTGCTTGAAGGGGAGGAAAAGGGTGCTGCAATAGTGCAAGAGGCAAAGCAACAAAGGGTGTCTTTATTGGTGGTGGGACAAAGAAAACGTTCTATTTTGTGGTGCTTGATGAGGAAGTGGACAAGAAAGAGAACCAAAAGTGGGGTTGCTGAGTATTGTATACAGAACTCACCTTGTATGACCATTGCAGTGAGGAGGAAGAATAAAAAACTTGGAGGGTATTTGATTACTACCAAACGCCATAAAAACTTTTGGCTTTTGGCTTAG
Protein sequence

>Msa1213140

MVRPRFKLHSLCVGHARTPRVRACSASSFGSKFANRTEFSSNGVQDSGRDNKGDESGNKVMVVVDSSFEAKGALDWALSHTIQSQDTVVLVHVARPLRGGDESDVKFNLKAYQLLLDMKSMCEMKKPGVLVNIVLLEGEEKGAAIVQEAKQQRVSLLVVGQRKRSILWCLMRKWTRKRTKSGVAEYCIQNSPCMTIAVRRKNKKLGGYLITTKRHKNFWLLA*