AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1221500

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1221500 MtrunA17_Chr4g0073221 94.505 182 8 2 1 182 1 180 5.76e-125 349
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 47 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCTTTCCTTATCATTTCT+TGG 0.158331 8_1:-85104730 None:intergenic
CATAGTTGATGGAATCATTT+TGG 0.169533 8_1:+85105267 Msa1221500:exon
AAAAGAGAGGCACTGGTTTA+AGG 0.190542 8_1:+85103951 Msa1221500:CDS
TTTCCGCTGCGCTTATTAAT+TGG 0.228675 8_1:-85103705 None:intergenic
TCTCCAAATCTTGTGCATTT+TGG 0.261971 8_1:+85103604 Msa1221500:five_prime_UTR
TTTGTATTGTAAACTTATTT+TGG 0.274568 8_1:+85105420 Msa1221500:three_prime_UTR
CGACCAAAATGCACAAGATT+TGG 0.286210 8_1:-85103607 None:intergenic
CTAAATTGCAGAACCTTTGC+AGG 0.304960 8_1:+85104706 Msa1221500:intron
ATAGAATATAATAACCTATA+CGG 0.309711 8_1:+85105304 Msa1221500:three_prime_UTR
CAACCAACAAGCTGATGATT+TGG 0.322790 8_1:+85104784 Msa1221500:CDS
AGATGTGTGTTTGAGGAATA+TGG 0.344846 8_1:-85104832 None:intergenic
ATTTGGAAAATTTGAACAAA+AGG 0.378564 8_1:+85104801 Msa1221500:CDS
TCTCCTCAACCCTCTGGCTC+TGG 0.381273 8_1:+85103795 Msa1221500:CDS
TTGAAGTTGTCCGACAAGTT+TGG 0.396601 8_1:-85104904 None:intergenic
TTTCCAAATCATCAGCTTGT+TGG 0.398691 8_1:-85104787 None:intergenic
TAAGGAGTATCTCAAATCTC+AGG 0.410718 8_1:+85103969 Msa1221500:CDS
TTTGAAACCTTTGAAGTTGT+TGG 0.421383 8_1:-85103829 None:intergenic
ATAAGGAAAGATGTTTCAAC+TGG 0.421549 8_1:+85104740 Msa1221500:CDS
AGTTTACAATACAAATTTGT+CGG 0.426331 8_1:-85105412 None:intergenic
CGATCAGAGATGTGTGTTTG+AGG 0.434499 8_1:-85104839 None:intergenic
GAAAGTGGAAGCTCAATAAC+AGG 0.437471 8_1:-85104006 None:intergenic
TTGAAACCTTTGAAGTTGTT+GGG 0.441400 8_1:-85103828 None:intergenic
TGATGAGAGCTCATAGTTGA+TGG 0.447746 8_1:+85105256 Msa1221500:CDS
CTTCTTTCTCCTCAACCCTC+TGG 0.450020 8_1:+85103789 Msa1221500:CDS
CCATCACCCAACAACTTCAA+AGG 0.455131 8_1:+85103822 Msa1221500:CDS
TAGAATATAATAACCTATAC+GGG 0.458221 8_1:+85105305 Msa1221500:three_prime_UTR
TGCAGGACCAAGAAATGATA+AGG 0.469946 8_1:+85104723 Msa1221500:CDS
CCTTTGAAGTTGTTGGGTGA+TGG 0.474376 8_1:-85103822 None:intergenic
CATCTCTGATCGCGTTACTC+AGG 0.475344 8_1:+85104850 Msa1221500:CDS
AAACCAGAGCCAGAGGGTTG+AGG 0.485771 8_1:-85103798 None:intergenic
CCTAAAACAACACCCCGTAT+AGG 0.488150 8_1:-85105318 None:intergenic
ATCATTTCTTGGTCCTGCAA+AGG 0.488339 8_1:-85104719 None:intergenic
AACCTATAAAAGAGAGGCAC+TGG 0.490418 8_1:+85103944 Msa1221500:CDS
TCGCGTTACTCAGGGACAAA+AGG 0.514346 8_1:+85104859 Msa1221500:CDS
ACACCGTGTACCAAACTTGT+CGG 0.523677 8_1:+85104894 Msa1221500:CDS
CTCTCATCAGCTGTGCGTTG+AGG 0.535770 8_1:-85105242 None:intergenic
TACCTACCTTGAGTTGAAAG+TGG 0.539797 8_1:-85104021 None:intergenic
ATCTCTGATCGCGTTACTCA+GGG 0.556344 8_1:+85104851 Msa1221500:CDS
GAGCTTCCACTTTCAACTCA+AGG 0.587387 8_1:+85104015 Msa1221500:CDS
AAGGAGTATCTCAAATCTCA+GGG 0.605928 8_1:+85103970 Msa1221500:CDS
GAGGGTTGAGGAGAAAGAAG+AGG 0.618199 8_1:-85103786 None:intergenic
GCGCAGCGGAAAGAAACCAT+TGG 0.621480 8_1:+85103716 Msa1221500:CDS
TGATGGAAAACCAGAGCCAG+AGG 0.641831 8_1:-85103805 None:intergenic
TGTCCGACAAGTTTGGTACA+CGG 0.684798 8_1:-85104897 None:intergenic
GATGGAAAACCAGAGCCAGA+GGG 0.688074 8_1:-85103804 None:intergenic
AAACCAATTAATAAGCGCAG+CGG 0.695563 8_1:+85103702 Msa1221500:CDS
AGAATATAATAACCTATACG+GGG 0.782218 8_1:+85105306 Msa1221500:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTGTATAATCATTTATTAAT+TGG + chr8_1:85104200-85104219 Msa1221500:intron 15.0%
!!! TATAATTTTCAATGTCATAT+AGG - chr8_1:85104481-85104500 None:intergenic 15.0%
!! AAAATTAGATTCAATGTTAA+TGG + chr8_1:85105015-85105034 Msa1221500:intron 15.0%
!!! TAATTAAGAATTATTTTGGA+TGG + chr8_1:85105178-85105197 Msa1221500:intron 15.0%
!! ATAGAATATAATAACCTATA+CGG + chr8_1:85105304-85105323 Msa1221500:three_prime_UTR 15.0%
!!! TTTGTATTGTAAACTTATTT+TGG + chr8_1:85105420-85105439 Msa1221500:three_prime_UTR 15.0%
!! GTGTATAATCATTTATTAAT+TGG + chr8_1:85104200-85104219 Msa1221500:intron 15.0%
!!! TATAATTTTCAATGTCATAT+AGG - chr8_1:85104481-85104500 None:intergenic 15.0%
!! AAAATTAGATTCAATGTTAA+TGG + chr8_1:85105015-85105034 Msa1221500:intron 15.0%
!!! TAATTAAGAATTATTTTGGA+TGG + chr8_1:85105178-85105197 Msa1221500:intron 15.0%
!! ATAGAATATAATAACCTATA+CGG + chr8_1:85105304-85105323 Msa1221500:three_prime_UTR 15.0%
!!! TTTGTATTGTAAACTTATTT+TGG + chr8_1:85105420-85105439 Msa1221500:three_prime_UTR 15.0%
!!! TTCTACTTTAATTGTTGTTA+TGG + chr8_1:85104066-85104085 Msa1221500:intron 20.0%
!!! TTTAATTGTTGTTATGGATA+TGG + chr8_1:85104072-85104091 Msa1221500:intron 20.0%
!!! AGAGACAATTTTAAAAGAAA+TGG + chr8_1:85104235-85104254 Msa1221500:intron 20.0%
!!! TTTAATTTACTCGTTCATAT+TGG - chr8_1:85104395-85104414 None:intergenic 20.0%
!!! CTATATACACTAAATCTTTT+GGG - chr8_1:85104458-85104477 None:intergenic 20.0%
!!! TTTTATGTTTCTTCTCAAAT+AGG - chr8_1:85104631-85104650 None:intergenic 20.0%
!! TTTAGCAGAAATGAATAATT+AGG - chr8_1:85104691-85104710 None:intergenic 20.0%
!! ATTTGGAAAATTTGAACAAA+AGG + chr8_1:85104801-85104820 Msa1221500:CDS 20.0%
!! TAGAATATAATAACCTATAC+GGG + chr8_1:85105305-85105324 Msa1221500:three_prime_UTR 20.0%
!! AGTTTACAATACAAATTTGT+CGG - chr8_1:85105415-85105434 None:intergenic 20.0%
!!! TTCTACTTTAATTGTTGTTA+TGG + chr8_1:85104066-85104085 Msa1221500:intron 20.0%
!!! TTTAATTGTTGTTATGGATA+TGG + chr8_1:85104072-85104091 Msa1221500:intron 20.0%
!!! AGAGACAATTTTAAAAGAAA+TGG + chr8_1:85104235-85104254 Msa1221500:intron 20.0%
!!! TTTAATTTACTCGTTCATAT+TGG - chr8_1:85104395-85104414 None:intergenic 20.0%
!!! CTATATACACTAAATCTTTT+GGG - chr8_1:85104458-85104477 None:intergenic 20.0%
!!! TTTTATGTTTCTTCTCAAAT+AGG - chr8_1:85104631-85104650 None:intergenic 20.0%
!! TTTAGCAGAAATGAATAATT+AGG - chr8_1:85104691-85104710 None:intergenic 20.0%
!! ATTTGGAAAATTTGAACAAA+AGG + chr8_1:85104801-85104820 Msa1221500:CDS 20.0%
!! TAGAATATAATAACCTATAC+GGG + chr8_1:85105305-85105324 Msa1221500:three_prime_UTR 20.0%
!! AGTTTACAATACAAATTTGT+CGG - chr8_1:85105415-85105434 None:intergenic 20.0%
! AATTGAAAAAGAAGCAATGA+AGG - chr8_1:85103883-85103902 None:intergenic 25.0%
!!! TTCATTGCTTCTTTTTCAAT+TGG + chr8_1:85103882-85103901 Msa1221500:intron 25.0%
!!! TCATTGCTTCTTTTTCAATT+GGG + chr8_1:85103883-85103902 Msa1221500:intron 25.0%
!!! TAATTTGTTTATGATCACAG+TGG + chr8_1:85103909-85103928 Msa1221500:intron 25.0%
! TGAAAAAACCTATAAAAGAG+AGG + chr8_1:85103938-85103957 Msa1221500:CDS 25.0%
! TAAAGATTATGTTAGCATTG+AGG + chr8_1:85104157-85104176 Msa1221500:intron 25.0%
! CATTTATTAATTGGATGCTA+AGG + chr8_1:85104209-85104228 Msa1221500:intron 25.0%
!!! GACAATTTTAAAAGAAATGG+TGG + chr8_1:85104238-85104257 Msa1221500:intron 25.0%
! AAAAGTTGAATCATTGAGTA+AGG + chr8_1:85104427-85104446 Msa1221500:intron 25.0%
!! GCTATATACACTAAATCTTT+TGG - chr8_1:85104459-85104478 None:intergenic 25.0%
!! ATTTTACTGATACTATGTTG+TGG + chr8_1:85104550-85104569 Msa1221500:intron 25.0%
! AGAATATAATAACCTATACG+GGG + chr8_1:85105306-85105325 Msa1221500:three_prime_UTR 25.0%
!! TATCATCTTTGTCTTTTGAT+AGG + chr8_1:85105380-85105399 Msa1221500:three_prime_UTR 25.0%
! AATTGAAAAAGAAGCAATGA+AGG - chr8_1:85103883-85103902 None:intergenic 25.0%
!!! TTCATTGCTTCTTTTTCAAT+TGG + chr8_1:85103882-85103901 Msa1221500:intron 25.0%
!!! TCATTGCTTCTTTTTCAATT+GGG + chr8_1:85103883-85103902 Msa1221500:intron 25.0%
!!! TAATTTGTTTATGATCACAG+TGG + chr8_1:85103909-85103928 Msa1221500:intron 25.0%
! TGAAAAAACCTATAAAAGAG+AGG + chr8_1:85103938-85103957 Msa1221500:CDS 25.0%
! TAAAGATTATGTTAGCATTG+AGG + chr8_1:85104157-85104176 Msa1221500:intron 25.0%
! CATTTATTAATTGGATGCTA+AGG + chr8_1:85104209-85104228 Msa1221500:intron 25.0%
!!! GACAATTTTAAAAGAAATGG+TGG + chr8_1:85104238-85104257 Msa1221500:intron 25.0%
! AAAAGTTGAATCATTGAGTA+AGG + chr8_1:85104427-85104446 Msa1221500:intron 25.0%
!! GCTATATACACTAAATCTTT+TGG - chr8_1:85104459-85104478 None:intergenic 25.0%
!! ATTTTACTGATACTATGTTG+TGG + chr8_1:85104550-85104569 Msa1221500:intron 25.0%
! AGAATATAATAACCTATACG+GGG + chr8_1:85105306-85105325 Msa1221500:three_prime_UTR 25.0%
!! TATCATCTTTGTCTTTTGAT+AGG + chr8_1:85105380-85105399 Msa1221500:three_prime_UTR 25.0%
GAAGAAGAAGAAGAAGAAAA+TGG + chr8_1:85103644-85103663 Msa1221500:exon 30.0%
!! TTGAAACCTTTGAAGTTGTT+GGG - chr8_1:85103831-85103850 None:intergenic 30.0%
!! TTTGAAACCTTTGAAGTTGT+TGG - chr8_1:85103832-85103851 None:intergenic 30.0%
! TTATGTTAGCATTGAGGATT+TGG + chr8_1:85104163-85104182 Msa1221500:intron 30.0%
TACCAATATGCTAGAAAAGA+GGG - chr8_1:85104329-85104348 None:intergenic 30.0%
CATCTTTCCTTATCATTTCT+TGG - chr8_1:85104733-85104752 None:intergenic 30.0%
ATAAGGAAAGATGTTTCAAC+TGG + chr8_1:85104740-85104759 Msa1221500:CDS 30.0%
ATGGTAGACACAATATACTA+TGG + chr8_1:85105034-85105053 Msa1221500:intron 30.0%
!!! GATTTTTTTTGTCCTTCTGA+TGG + chr8_1:85105066-85105085 Msa1221500:intron 30.0%
! AGGAAACTTGATCATTTTGA+TGG + chr8_1:85105104-85105123 Msa1221500:intron 30.0%
TACTTCTATATATATGCTGC+AGG + chr8_1:85105210-85105229 Msa1221500:intron 30.0%
!!! CATAGTTGATGGAATCATTT+TGG + chr8_1:85105267-85105286 Msa1221500:exon 30.0%
GAAGAAGAAGAAGAAGAAAA+TGG + chr8_1:85103644-85103663 Msa1221500:exon 30.0%
!! TTGAAACCTTTGAAGTTGTT+GGG - chr8_1:85103831-85103850 None:intergenic 30.0%
!! TTTGAAACCTTTGAAGTTGT+TGG - chr8_1:85103832-85103851 None:intergenic 30.0%
! TTATGTTAGCATTGAGGATT+TGG + chr8_1:85104163-85104182 Msa1221500:intron 30.0%
TACCAATATGCTAGAAAAGA+GGG - chr8_1:85104329-85104348 None:intergenic 30.0%
CATCTTTCCTTATCATTTCT+TGG - chr8_1:85104733-85104752 None:intergenic 30.0%
ATAAGGAAAGATGTTTCAAC+TGG + chr8_1:85104740-85104759 Msa1221500:CDS 30.0%
ATGGTAGACACAATATACTA+TGG + chr8_1:85105034-85105053 Msa1221500:intron 30.0%
!!! GATTTTTTTTGTCCTTCTGA+TGG + chr8_1:85105066-85105085 Msa1221500:intron 30.0%
! AGGAAACTTGATCATTTTGA+TGG + chr8_1:85105104-85105123 Msa1221500:intron 30.0%
TACTTCTATATATATGCTGC+AGG + chr8_1:85105210-85105229 Msa1221500:intron 30.0%
!!! CATAGTTGATGGAATCATTT+TGG + chr8_1:85105267-85105286 Msa1221500:exon 30.0%
! TCTCCAAATCTTGTGCATTT+TGG + chr8_1:85103604-85103623 Msa1221500:five_prime_UTR 35.0%
!! CAAATCTTGTGCATTTTGGT+CGG + chr8_1:85103608-85103627 Msa1221500:five_prime_UTR 35.0%
AAACCAATTAATAAGCGCAG+CGG + chr8_1:85103702-85103721 Msa1221500:CDS 35.0%
! ACCATTGGTGAAGAAGTTTT+TGG + chr8_1:85103731-85103750 Msa1221500:CDS 35.0%
TCCAAAAACTTCTTCACCAA+TGG - chr8_1:85103735-85103754 None:intergenic 35.0%
TTTGTTTATGATCACAGTGG+TGG + chr8_1:85103912-85103931 Msa1221500:intron 35.0%
!! TAAGGAGTATCTCAAATCTC+AGG + chr8_1:85103969-85103988 Msa1221500:CDS 35.0%
!! AAGGAGTATCTCAAATCTCA+GGG + chr8_1:85103970-85103989 Msa1221500:CDS 35.0%
! AGCATTGAGGATTTGGAATT+AGG + chr8_1:85104170-85104189 Msa1221500:intron 35.0%
! GAGGATTTGGAATTAGGAAA+AGG + chr8_1:85104176-85104195 Msa1221500:intron 35.0%
!! AACCCTCTTTTCTAGCATAT+TGG + chr8_1:85104324-85104343 Msa1221500:intron 35.0%
CTACCAATATGCTAGAAAAG+AGG - chr8_1:85104330-85104349 None:intergenic 35.0%
!! GCATATTGGTAGCTTTATGT+TGG + chr8_1:85104338-85104357 Msa1221500:intron 35.0%
ACTCGTTCATATTGGACTAT+TGG - chr8_1:85104387-85104406 None:intergenic 35.0%
TTACTGATACTATGTTGTGG+AGG + chr8_1:85104553-85104572 Msa1221500:intron 35.0%
TTTCCAAATCATCAGCTTGT+TGG - chr8_1:85104790-85104809 None:intergenic 35.0%
AGATGTGTGTTTGAGGAATA+TGG - chr8_1:85104835-85104854 None:intergenic 35.0%
! TTCCAAGTTGACTTAGCATA+AGG + chr8_1:85105138-85105157 Msa1221500:intron 35.0%
TTCCTTATGCTAAGTCAACT+TGG - chr8_1:85105143-85105162 None:intergenic 35.0%
! TCTCCAAATCTTGTGCATTT+TGG + chr8_1:85103604-85103623 Msa1221500:five_prime_UTR 35.0%
!! CAAATCTTGTGCATTTTGGT+CGG + chr8_1:85103608-85103627 Msa1221500:five_prime_UTR 35.0%
AAACCAATTAATAAGCGCAG+CGG + chr8_1:85103702-85103721 Msa1221500:CDS 35.0%
! ACCATTGGTGAAGAAGTTTT+TGG + chr8_1:85103731-85103750 Msa1221500:CDS 35.0%
TCCAAAAACTTCTTCACCAA+TGG - chr8_1:85103735-85103754 None:intergenic 35.0%
TTTGTTTATGATCACAGTGG+TGG + chr8_1:85103912-85103931 Msa1221500:intron 35.0%
!! TAAGGAGTATCTCAAATCTC+AGG + chr8_1:85103969-85103988 Msa1221500:CDS 35.0%
!! AAGGAGTATCTCAAATCTCA+GGG + chr8_1:85103970-85103989 Msa1221500:CDS 35.0%
! AGCATTGAGGATTTGGAATT+AGG + chr8_1:85104170-85104189 Msa1221500:intron 35.0%
! GAGGATTTGGAATTAGGAAA+AGG + chr8_1:85104176-85104195 Msa1221500:intron 35.0%
!! AACCCTCTTTTCTAGCATAT+TGG + chr8_1:85104324-85104343 Msa1221500:intron 35.0%
CTACCAATATGCTAGAAAAG+AGG - chr8_1:85104330-85104349 None:intergenic 35.0%
!! GCATATTGGTAGCTTTATGT+TGG + chr8_1:85104338-85104357 Msa1221500:intron 35.0%
ACTCGTTCATATTGGACTAT+TGG - chr8_1:85104387-85104406 None:intergenic 35.0%
TTACTGATACTATGTTGTGG+AGG + chr8_1:85104553-85104572 Msa1221500:intron 35.0%
TTTCCAAATCATCAGCTTGT+TGG - chr8_1:85104790-85104809 None:intergenic 35.0%
AGATGTGTGTTTGAGGAATA+TGG - chr8_1:85104835-85104854 None:intergenic 35.0%
! TTCCAAGTTGACTTAGCATA+AGG + chr8_1:85105138-85105157 Msa1221500:intron 35.0%
TTCCTTATGCTAAGTCAACT+TGG - chr8_1:85105143-85105162 None:intergenic 35.0%
CGACCAAAATGCACAAGATT+TGG - chr8_1:85103610-85103629 None:intergenic 40.0%
TTTCCGCTGCGCTTATTAAT+TGG - chr8_1:85103708-85103727 None:intergenic 40.0%
! AACCTATAAAAGAGAGGCAC+TGG + chr8_1:85103944-85103963 Msa1221500:CDS 40.0%
! AACCAGTGCCTCTCTTTTAT+AGG - chr8_1:85103949-85103968 None:intergenic 40.0%
!! AAAAGAGAGGCACTGGTTTA+AGG + chr8_1:85103951-85103970 Msa1221500:CDS 40.0%
GAAAGTGGAAGCTCAATAAC+AGG - chr8_1:85104009-85104028 None:intergenic 40.0%
TTCCACTTTCAACTCAAGGT+AGG + chr8_1:85104019-85104038 Msa1221500:intron 40.0%
!! TACCTACCTTGAGTTGAAAG+TGG - chr8_1:85104024-85104043 None:intergenic 40.0%
TAGCTGCTGAATTTGTGTCA+TGG + chr8_1:85104126-85104145 Msa1221500:intron 40.0%
GTGAGACAAGGTAACAATGT+TGG - chr8_1:85104519-85104538 None:intergenic 40.0%
TCATACGAAGAAGTGAGACA+AGG - chr8_1:85104531-85104550 None:intergenic 40.0%
CTAAATTGCAGAACCTTTGC+AGG + chr8_1:85104706-85104725 Msa1221500:intron 40.0%
ATCATTTCTTGGTCCTGCAA+AGG - chr8_1:85104722-85104741 None:intergenic 40.0%
TGCAGGACCAAGAAATGATA+AGG + chr8_1:85104723-85104742 Msa1221500:CDS 40.0%
CAACCAACAAGCTGATGATT+TGG + chr8_1:85104784-85104803 Msa1221500:CDS 40.0%
TTGAAGTTGTCCGACAAGTT+TGG - chr8_1:85104907-85104926 None:intergenic 40.0%
!! TAGTCTTGTCTTCCATCAGA+AGG - chr8_1:85105081-85105100 None:intergenic 40.0%
GACTTAGCATAAGGAATCTG+AGG + chr8_1:85105147-85105166 Msa1221500:intron 40.0%
TGATGAGAGCTCATAGTTGA+TGG + chr8_1:85105256-85105275 Msa1221500:CDS 40.0%
CGACCAAAATGCACAAGATT+TGG - chr8_1:85103610-85103629 None:intergenic 40.0%
TTTCCGCTGCGCTTATTAAT+TGG - chr8_1:85103708-85103727 None:intergenic 40.0%
! AACCTATAAAAGAGAGGCAC+TGG + chr8_1:85103944-85103963 Msa1221500:CDS 40.0%
! AACCAGTGCCTCTCTTTTAT+AGG - chr8_1:85103949-85103968 None:intergenic 40.0%
!! AAAAGAGAGGCACTGGTTTA+AGG + chr8_1:85103951-85103970 Msa1221500:CDS 40.0%
GAAAGTGGAAGCTCAATAAC+AGG - chr8_1:85104009-85104028 None:intergenic 40.0%
TTCCACTTTCAACTCAAGGT+AGG + chr8_1:85104019-85104038 Msa1221500:intron 40.0%
!! TACCTACCTTGAGTTGAAAG+TGG - chr8_1:85104024-85104043 None:intergenic 40.0%
TAGCTGCTGAATTTGTGTCA+TGG + chr8_1:85104126-85104145 Msa1221500:intron 40.0%
GTGAGACAAGGTAACAATGT+TGG - chr8_1:85104519-85104538 None:intergenic 40.0%
TCATACGAAGAAGTGAGACA+AGG - chr8_1:85104531-85104550 None:intergenic 40.0%
CTAAATTGCAGAACCTTTGC+AGG + chr8_1:85104706-85104725 Msa1221500:intron 40.0%
ATCATTTCTTGGTCCTGCAA+AGG - chr8_1:85104722-85104741 None:intergenic 40.0%
TGCAGGACCAAGAAATGATA+AGG + chr8_1:85104723-85104742 Msa1221500:CDS 40.0%
CAACCAACAAGCTGATGATT+TGG + chr8_1:85104784-85104803 Msa1221500:CDS 40.0%
TTGAAGTTGTCCGACAAGTT+TGG - chr8_1:85104907-85104926 None:intergenic 40.0%
!! TAGTCTTGTCTTCCATCAGA+AGG - chr8_1:85105081-85105100 None:intergenic 40.0%
GACTTAGCATAAGGAATCTG+AGG + chr8_1:85105147-85105166 Msa1221500:intron 40.0%
TGATGAGAGCTCATAGTTGA+TGG + chr8_1:85105256-85105275 Msa1221500:CDS 40.0%
!! CCTTTGAAGTTGTTGGGTGA+TGG - chr8_1:85103825-85103844 None:intergenic 45.0%
CCATCACCCAACAACTTCAA+AGG + chr8_1:85103822-85103841 Msa1221500:CDS 45.0%
GAGCTTCCACTTTCAACTCA+AGG + chr8_1:85104015-85104034 Msa1221500:CDS 45.0%
! TTGTGGAGGCATATAAACCG+TGG + chr8_1:85104567-85104586 Msa1221500:intron 45.0%
CGATCAGAGATGTGTGTTTG+AGG - chr8_1:85104842-85104861 None:intergenic 45.0%
ATCTCTGATCGCGTTACTCA+GGG + chr8_1:85104851-85104870 Msa1221500:CDS 45.0%
ACACCGTGTACCAAACTTGT+CGG + chr8_1:85104894-85104913 Msa1221500:CDS 45.0%
TGTCCGACAAGTTTGGTACA+CGG - chr8_1:85104900-85104919 None:intergenic 45.0%
! GATGGAAGACAAGACTAGCT+AGG + chr8_1:85105084-85105103 Msa1221500:intron 45.0%
CCTAAAACAACACCCCGTAT+AGG - chr8_1:85105321-85105340 None:intergenic 45.0%
!!! CCTATACGGGGTGTTGTTTT+AGG + chr8_1:85105318-85105337 Msa1221500:three_prime_UTR 45.0%
!! CCTTTGAAGTTGTTGGGTGA+TGG - chr8_1:85103825-85103844 None:intergenic 45.0%
CCATCACCCAACAACTTCAA+AGG + chr8_1:85103822-85103841 Msa1221500:CDS 45.0%
GAGCTTCCACTTTCAACTCA+AGG + chr8_1:85104015-85104034 Msa1221500:CDS 45.0%
! TTGTGGAGGCATATAAACCG+TGG + chr8_1:85104567-85104586 Msa1221500:intron 45.0%
CGATCAGAGATGTGTGTTTG+AGG - chr8_1:85104842-85104861 None:intergenic 45.0%
ATCTCTGATCGCGTTACTCA+GGG + chr8_1:85104851-85104870 Msa1221500:CDS 45.0%
ACACCGTGTACCAAACTTGT+CGG + chr8_1:85104894-85104913 Msa1221500:CDS 45.0%
TGTCCGACAAGTTTGGTACA+CGG - chr8_1:85104900-85104919 None:intergenic 45.0%
! GATGGAAGACAAGACTAGCT+AGG + chr8_1:85105084-85105103 Msa1221500:intron 45.0%
CCTAAAACAACACCCCGTAT+AGG - chr8_1:85105321-85105340 None:intergenic 45.0%
!!! CCTATACGGGGTGTTGTTTT+AGG + chr8_1:85105318-85105337 Msa1221500:three_prime_UTR 45.0%
!!! ATTTTAATTAAGAATTATTT+TGG + chr8_1:85105174-85105193 Msa1221500:intron 5.0%
!!! ATTTTAATTAAGAATTATTT+TGG + chr8_1:85105174-85105193 Msa1221500:intron 5.0%
GAGGGTTGAGGAGAAAGAAG+AGG - chr8_1:85103789-85103808 None:intergenic 50.0%
CTTCTTTCTCCTCAACCCTC+TGG + chr8_1:85103789-85103808 Msa1221500:CDS 50.0%
GATGGAAAACCAGAGCCAGA+GGG - chr8_1:85103807-85103826 None:intergenic 50.0%
TGATGGAAAACCAGAGCCAG+AGG - chr8_1:85103808-85103827 None:intergenic 50.0%
CATATAAACCGTGGCGCTCT+TGG + chr8_1:85104576-85104595 Msa1221500:intron 50.0%
!! ATCTATCACCAAGAGCGCCA+CGG - chr8_1:85104587-85104606 None:intergenic 50.0%
CATCTCTGATCGCGTTACTC+AGG + chr8_1:85104850-85104869 Msa1221500:CDS 50.0%
TCGCGTTACTCAGGGACAAA+AGG + chr8_1:85104859-85104878 Msa1221500:CDS 50.0%
GAGGGTTGAGGAGAAAGAAG+AGG - chr8_1:85103789-85103808 None:intergenic 50.0%
CTTCTTTCTCCTCAACCCTC+TGG + chr8_1:85103789-85103808 Msa1221500:CDS 50.0%
GATGGAAAACCAGAGCCAGA+GGG - chr8_1:85103807-85103826 None:intergenic 50.0%
TGATGGAAAACCAGAGCCAG+AGG - chr8_1:85103808-85103827 None:intergenic 50.0%
CATATAAACCGTGGCGCTCT+TGG + chr8_1:85104576-85104595 Msa1221500:intron 50.0%
!! ATCTATCACCAAGAGCGCCA+CGG - chr8_1:85104587-85104606 None:intergenic 50.0%
CATCTCTGATCGCGTTACTC+AGG + chr8_1:85104850-85104869 Msa1221500:CDS 50.0%
TCGCGTTACTCAGGGACAAA+AGG + chr8_1:85104859-85104878 Msa1221500:CDS 50.0%
GCGCAGCGGAAAGAAACCAT+TGG + chr8_1:85103716-85103735 Msa1221500:CDS 55.0%
AAACCAGAGCCAGAGGGTTG+AGG - chr8_1:85103801-85103820 None:intergenic 55.0%
CTCTCATCAGCTGTGCGTTG+AGG - chr8_1:85105245-85105264 None:intergenic 55.0%
GCGCAGCGGAAAGAAACCAT+TGG + chr8_1:85103716-85103735 Msa1221500:CDS 55.0%
AAACCAGAGCCAGAGGGTTG+AGG - chr8_1:85103801-85103820 None:intergenic 55.0%
CTCTCATCAGCTGTGCGTTG+AGG - chr8_1:85105245-85105264 None:intergenic 55.0%
! TCTCCTCAACCCTCTGGCTC+TGG + chr8_1:85103795-85103814 Msa1221500:CDS 60.0%
! TCTCCTCAACCCTCTGGCTC+TGG + chr8_1:85103795-85103814 Msa1221500:CDS 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 85103601 85105469 85103601 ID=Msa1221500;Name=Msa1221500
chr8_1 mRNA 85103601 85105469 85103601 ID=Msa1221500-mRNA-1;Parent=Msa1221500;Name=Msa1221500-mRNA-1;_AED=0.07;_eAED=0.07;_QI=62|1|0.75|1|0.33|1|4|194|182
chr8_1 exon 85103601 85103843 85103601 ID=Msa1221500-mRNA-1:exon:30622;Parent=Msa1221500-mRNA-1
chr8_1 exon 85103929 85104036 85103929 ID=Msa1221500-mRNA-1:exon:30623;Parent=Msa1221500-mRNA-1
chr8_1 exon 85104717 85104932 85104717 ID=Msa1221500-mRNA-1:exon:30624;Parent=Msa1221500-mRNA-1
chr8_1 exon 85105232 85105469 85105232 ID=Msa1221500-mRNA-1:exon:30625;Parent=Msa1221500-mRNA-1
chr8_1 five_prime_UTR 85103601 85103662 85103601 ID=Msa1221500-mRNA-1:five_prime_utr;Parent=Msa1221500-mRNA-1
chr8_1 CDS 85103663 85103843 85103663 ID=Msa1221500-mRNA-1:cds;Parent=Msa1221500-mRNA-1
chr8_1 CDS 85103929 85104036 85103929 ID=Msa1221500-mRNA-1:cds;Parent=Msa1221500-mRNA-1
chr8_1 CDS 85104717 85104932 85104717 ID=Msa1221500-mRNA-1:cds;Parent=Msa1221500-mRNA-1
chr8_1 CDS 85105232 85105275 85105232 ID=Msa1221500-mRNA-1:cds;Parent=Msa1221500-mRNA-1
chr8_1 three_prime_UTR 85105276 85105469 85105276 ID=Msa1221500-mRNA-1:three_prime_utr;Parent=Msa1221500-mRNA-1
Gene Sequence

>Msa1221500

ATGGATATCAGAAATCATCGTTTAAGAGTTCAAAACAAGAAACCAATTAATAAGCGCAGCGGAAAGAAACCATTGGTGAAGAAGTTTTTGGATTATCTTAAGTCTGATACTTTTTTGTATGCTCCTCTTCTTTCTCCTCAACCCTCTGGCTCTGGTTTTCCATCACCCAACAACTTCAAAGTGGTGGAGCTGAAAAAACCTATAAAAGAGAGGCACTGGTTTAAGGAGTATCTCAAATCTCAGGGCTATATGTATGATCCTGTTATTGAGCTTCCACTTTCAACTCAAGAACCTTTGCAGGACCAAGAAATGATAAGGAAAGATGTTTCAACTGGAAAGTCAACAATGAATGTCAACAACCAACAAGCTGATGATTTGGAAAATTTGAACAAAAGGTCTGAAAGCCATATTCCTCAAACACACATCTCTGATCGCGTTACTCAGGGACAAAAGGAAACTGTGAAGCACACCGTGTACCAAACTTGTCGGACAACTTCAACTTCAAGAAATATGATCCTCAACGCACAGCTGATGAGAGCTCATAGTTGA
Protein sequence

>Msa1221500

MDIRNHRLRVQNKKPINKRSGKKPLVKKFLDYLKSDTFLYAPLLSPQPSGSGFPSPNNFKVVELKKPIKERHWFKEYLKSQGYMYDPVIELPLSTQEPLQDQEMIRKDVSTGKSTMNVNNQQADDLENLNKRSESHIPQTHISDRVTQGQKETVKHTVYQTCRTTSTSRNMILNAQLMRAHS*