AlfalfaGEDB | Msa0975460

M. sativa cultivar zhongmu-4 / Msa0975460

KEGG: K21374
GO: GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0008194 GO:0015020 GO:0016740 GO:0016757 GO:0016758 GO:0035251 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0046527 GO:0080043 GO:0080044
PFAM: PF00201.21

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0975460	XP_003618658.3	85.208	480	39	2	1	448	1	480	0.0	827

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0975460	sp\|F8WKW1\|UGT2_GARJA	55.579	475	177	5	7	448	8	481	0.0	538

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0975460	G7KM79	85.208	480	39	2	1	448	1	480	0.0	827

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000180	Msa0975460	0.804142	2.489878e-49	-8.615850e-47
Msa0000220	Msa0975460	0.824048	9.968910e-54	-8.615850e-47
Msa0001040	Msa0975460	0.826467	2.670310e-54	-8.615850e-47
Msa0001510	Msa0975460	0.827359	1.634383e-54	-8.615850e-47
Msa0002110	Msa0975460	0.821957	3.063208e-53	-8.615850e-47
Msa0002690	Msa0975460	0.869883	2.216837e-66	-8.615850e-47
Msa0002700	Msa0975460	0.862929	3.578031e-64	-8.615850e-47
Msa0003340	Msa0975460	0.841817	3.775371e-58	-8.615850e-47
Msa0003370	Msa0975460	0.809676	1.681720e-50	-8.615850e-47
Msa0004180	Msa0975460	0.837355	5.458953e-57	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0975460	MtrunA17_Chr6g0454301	85.208	480	39	2	1	448	254	733	0.0	827
Msa0975460	MtrunA17_Chr6g0454301	77.729	229	50	1	216	444	1	228	4.72e-123	374
Msa0975460	MtrunA17_Chr8g0336331	76.250	480	81	3	1	448	1	479	0.0	749
Msa0975460	MtrunA17_Chr6g0454341	74.167	480	92	2	1	448	1	480	0.0	746
Msa0975460	MtrunA17_Chr3g0078841	74.792	480	89	2	1	448	1	480	0.0	743
Msa0975460	MtrunA17_Chr6g0454321	73.305	472	94	2	1	440	1	472	0.0	722
Msa0975460	MtrunA17_Chr6g0454321	67.769	121	30	2	5	116	499	619	7.37e-42	157
Msa0975460	MtrunA17_Chr6g0454401	71.281	484	102	4	1	448	1	483	0.0	690
Msa0975460	MtrunA17_Chr6g0454181	68.737	483	115	4	1	448	1	482	0.0	677
Msa0975460	MtrunA17_Chr6g0454191	69.008	484	113	4	1	448	1	483	0.0	675

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0975460	AT1G22380.1	52.008	473	195	4	7	447	10	482	0.0	520
Msa0975460	AT1G22360.1	51.357	479	201	4	1	447	1	479	0.0	515
Msa0975460	AT1G22340.1	52.000	475	195	5	7	448	10	484	0.0	514
Msa0975460	AT1G22400.1	49.474	475	207	4	6	447	9	483	1.65e-176	503
Msa0975460	AT1G22370.2	52.119	472	195	5	5	447	8	477	2.74e-173	494
Msa0975460	AT1G22360.2	49.478	479	198	5	1	447	1	467	2.52e-171	489
Msa0975460	AT1G78270.1	48.434	479	211	6	2	446	5	481	5.36e-168	481
Msa0975460	AT1G22370.1	54.181	299	131	3	153	447	11	307	4.28e-110	327
Msa0975460	AT5G59590.1	34.052	464	254	11	7	442	7	446	7.51e-82	259
Msa0975460	AT3G11340.1	32.321	461	268	10	8	446	7	445	2.85e-80	255

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Find 95 sgRNAs with CRISPR-Local

Find 294 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCAGGGATACCATCTA+AGG	0.446365	6_4:+9249396	None:intergenic
AAACGTTTGCTCAAATCAAG+AGG	0.611549	6_4:-9249427	Msa0975460:CDS
AAACTGTTAGAGTTTGCTTG+GGG	0.652785	6_4:-9246826	Msa0975460:CDS
AACAACTGATCCTGGTTCCT+TGG	0.422428	6_4:+9246888	None:intergenic
AACATGCACTGGCTGGAAAA+AGG	0.279272	6_4:+9249140	None:intergenic
AACTCCACAAATTGATCAAT+TGG	0.329261	6_4:-9246968	Msa0975460:CDS
AAGATACCGAGTGTCTTGAA+TGG	0.570424	6_4:-9246918	Msa0975460:CDS
AAGTCTAACATAAAATCATT+TGG	0.425125	6_4:+9247138	None:intergenic
AATAACAAACATGCACTGGC+TGG	0.491580	6_4:+9249133	None:intergenic
AATAGTGAGTTGGTGTCCGC+AGG	0.527162	6_4:-9246692	Msa0975460:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GGATTTATATGTCCTTGAAA+TGG	+	chr6_4:9246390-9246409	None:intergenic	30.0%
GAAGTTCACAAAAAGGTTTA+AGG	+	chr6_4:9246638-9246657	None:intergenic	30.0%
CTTGTAAGAAGTTCACAAAA+AGG	+	chr6_4:9246645-9246664	None:intergenic	30.0%
TCAGAAAATAAGCAAGTAAC+TGG	+	chr6_4:9246693-9246712	None:intergenic	30.0%
CTGGAAAAAGGATAACATTT+GGG	+	chr6_4:9246761-9246780	None:intergenic	30.0%
TTACGTTCCTTTGTAGAAAA+AGG	-	chr6_4:9246813-9246832	Msa0975460:CDS	30.0%
GTAGAAGAAATAGAGGAAAA+TGG	-	chr6_4:9247228-9247247	Msa0975460:intron	30.0%
CTAAAAAATTGTATTGGAGG+AGG	-	chr6_4:9247313-9247332	Msa0975460:intron	30.0%
TTATATCGAGAGTTACTCTT+AGG	+	chr6_4:9247586-9247605	None:intergenic	30.0%
TTTATCGATCAAATTTGTGG+AGG	+	chr6_4:9247691-9247710	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_4	gene	9246339	9249593	9246339	ID=Msa0975460;Name=Msa0975460
chr6_4	mRNA	9246339	9249593	9246339	ID=Msa0975460-mRNA-1;Parent=Msa0975460;Name=Msa0975460-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0\|0\|0\|1\|0\|0\|3\|0\|448
chr6_4	exon	9249374	9249593	9249374	ID=Msa0975460-mRNA-1:exon:3311;Parent=Msa0975460-mRNA-1
chr6_4	exon	9249116	9249355	9249116	ID=Msa0975460-mRNA-1:exon:3310;Parent=Msa0975460-mRNA-1
chr6_4	exon	9246339	9247225	9246339	ID=Msa0975460-mRNA-1:exon:3309;Parent=Msa0975460-mRNA-1
chr6_4	CDS	9249374	9249593	9249374	ID=Msa0975460-mRNA-1:cds;Parent=Msa0975460-mRNA-1
chr6_4	CDS	9249116	9249355	9249116	ID=Msa0975460-mRNA-1:cds;Parent=Msa0975460-mRNA-1
chr6_4	CDS	9246339	9247225	9246339	ID=Msa0975460-mRNA-1:cds;Parent=Msa0975460-mRNA-1

Gene Sequence

>Msa0975460

ATGGGAAACTTTGCAAACAGAAAACCACATGCTGTGTTGATTCCAGCTCCATTTCAAGGACATATAAATCCATTGTTCAAACTAGCAAAACTTCTTCACCTTAGAGGCTTTTACATAACCTTTGTCAACACTGAATACAATCATAAACGTTTGCTCAAATCAAGAGGTGCAAATGCCTTAGATGGTATCCCTGGTTTTAGCTTTGAAACCATACCAGATGGTGATGGTGATGTTAGTCAAGACATACCCTCTCTTGCTCAATCTATAAGAAAGAACTTCCTTAAACCTTTTTGTGAACTTCTTACAAGACTTAATCACTCTGCCAATGTTCCACCAGTTACTTGCTTATTTTCTGATTATTTTTTGAGCTTTACTATACAAGCTGGTGAAGAATTTGCAATCCCAAATGTTATCCTTTTTCCAGCCAGTGCATGTTTGTTATTGAGTATTCTTCACTTACATTGGATTCCAGGTTTGAAAAACTTTCGATTGAAGGACATTGTTGACTATATCAGGACAACAGATCCAAATGATTTTATGTTAGACTTTGTAATTGATGTGGCTGATAAAGTTCATAAAAACTCTACTATTATTTTGAATACTTTCAATGAACTTGAGAGTGATGTAATAAATGCTCTCTCCTCTATGTTTCCTTCTCTTTACACCTTTGGCCCTTTACCTTTATTATTAAACCAAACTCCACAAATTGATCAATTGGATTCTTTAGGTTCCAACATATGGAAAGAAGATACCGAGTGTCTTGAATGGCTTGGATCCAAGGAACCAGGATCAGTTGTTTATGTGAATTTCGGCAGCCTTACAGTTATGACTGAAGAGAAACTGTTAGAGTTTGCTTGGGGTTTGGCCAGTTGTAATAAACCTTTTTTGTGGATCATTAGGCCTGATCTTGTCACTGGTGGCTCAAAAGTTTTGTCATCAGAGTTTGTTAATGAAATTTCAGATAGAGGCCTAATAGTGAGTTGGTGTCCGCAGGAGCAAATTTTAAATCATCCTTCAGTTGGTGGATTCTTGACTCATTGTGGATGGAACTCAACCACTGAAAGCATATGTGCTGGAGTTCCAATGTTGTGTTGGCCATTTTTCACTGATCAGCCAACAAACTGTAGACTTATTTGTAATGAATGGGAGATTGGGATGGAAATTGATACGAATGTAAAGAGAGAGGAGGTGGAGAAGCTTGTCAATGAATTGATGGTGGGGGAGAAAGGAAAGAAGATGAGGAAAAAGGCAATCGAGTTGAAGAAGAAGGCGGAGGAGAACACGAGACCTGGAGGTTGTTCATACATGAACTTGGACAAATTGATCAAGGAGGTGTTGCTTAAATAA

Protein sequence

>Msa0975460

MGNFANRKPHAVLIPAPFQGHINPLFKLAKLLHLRGFYITFVNTEYNHKRLLKSRGANALDGIPGFSFETIPDGDGDVSQDIPSLAQSIRKNFLKPFCELLTRLNHSANVPPVTCLFSDYFLSFTIQAGEEFAIPNVILFPASACLLLSILHLHWIPGLKNFRLKDIVDYIRTTDPNDFMLDFVIDVADKVHKNSTIILNTFNELESDVINALSSMFPSLYTFGPLPLLLNQTPQIDQLDSLGSNIWKEDTECLEWLGSKEPGSVVYVNFGSLTVMTEEKLLEFAWGLASCNKPFLWIIRPDLVTGGSKVLSSEFVNEISDRGLIVSWCPQEQILNHPSVGGFLTHCGWNSTTESICAGVPMLCWPFFTDQPTNCRLICNEWEIGMEIDTNVKREEVEKLVNELMVGEKGKKMRKKAIELKKKAEENTRPGGCSYMNLDKLIKEVLLK*