AlfalfaGEDB | Msa1170840

M. sativa cultivar zhongmu-4 / Msa1170840

KEGG: K14496
GO: GO:0001101 GO:0003674 GO:0004857 GO:0004864 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0007154 GO:0007165 GO:0008150 GO:0008289 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009753 GO:0009755 GO:0009867 GO:0009892 GO:0009987 GO:0010033 GO:0010427 GO:0010563 GO:0010605 GO:0010921 GO:0010923 GO:0019208 GO:0019212 GO:0019220 GO:0019222 GO:0019840 GO:0019888 GO:0023052 GO:0030234 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0031406 GO:0032268 GO:0032269 GO:0032515 GO:0032870 GO:0033293 GO:0033993 GO:0035303 GO:0035304 GO:0035305 GO:0035308 GO:0036094 GO:0038023 GO:0042221 GO:0042562 GO:0042802 GO:0042803 GO:0043086 GO:0043167 GO:0043168 GO:0043177 GO:0043178 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043666 GO:0044092 GO:0044424 GO:0044464 GO:0045936 GO:0046983 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051174 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0051716 GO:0060089 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071395 GO:0071396 GO:0071495 GO:0080090 GO:0097305 GO:0097306 GO:0098772 GO:1901700 GO:1901701
PFAM: PF10604.12

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1170840	sp\|Q8S8E3\|PYL6_ARATH	58.571	210	79	3	1	203	1	209	1.61e-80	242

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1170840	G7K772	95.610	205	9	0	1	205	1	205	1.03e-142	405

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0629220	Msa1170840	0.893380	6.631205e-75	-8.615850e-47
Msa0667010	Msa1170840	0.905867	2.726047e-80	-8.615850e-47
Msa0738680	Msa1170840	0.812161	4.873709e-51	-8.615850e-47
Msa0819150	Msa1170840	0.810710	1.006664e-50	-8.615850e-47
Msa0373110	Msa1170840	-0.812507	4.095757e-51	-8.615850e-47
Msa0413590	Msa1170840	-0.807559	4.765561e-50	-8.615850e-47
Msa0415470	Msa1170840	0.804350	2.253449e-49	-8.615850e-47
Msa0429050	Msa1170840	0.805878	1.078973e-49	-8.615850e-47
Msa0467480	Msa1170840	-0.818298	2.108794e-52	-8.615850e-47
Msa1025270	Msa1170840	0.808753	2.653239e-50	-8.615850e-47
Msa1050140	Msa1170840	0.835312	1.805521e-56	-8.615850e-47
Msa1088460	Msa1170840	0.835103	2.038283e-56	-8.615850e-47
Msa1153000	Msa1170840	0.804383	2.218323e-49	-8.615850e-47
Msa1170840	Msa1221500	-0.806762	7.027106e-50	-8.615850e-47
Msa1170840	Msa1259560	0.800119	1.673480e-48	-8.615850e-47
Msa1170840	Msa1299280	0.811499	6.790751e-51	-8.615850e-47
Msa1170840	Msa1432460	0.816575	5.151646e-52	-8.615850e-47
Msa1170840	Msa1439590	0.813340	2.689202e-51	-8.615850e-47
Msa1170840	Msa1445040	0.982529	2.504896e-155	-8.615850e-47
Msa1170840	Msa1458530	0.832838	7.520153e-56	-8.615850e-47
Msa0532210	Msa1170840	0.906262	1.789852e-80	-8.615850e-47
Msa0945330	Msa1170840	0.801768	7.702970e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1170840	MtrunA17_Chr5g0437611	95.610	205	9	0	1	205	1	205	1.98e-146	405
Msa1170840	MtrunA17_Chr3g0114121	62.946	224	62	5	1	204	1	223	4.31e-84	248
Msa1170840	MtrunA17_Chr4g0005501	64.327	171	54	4	39	205	42	209	9.23e-72	216
Msa1170840	MtrunA17_Chr7g0243271	68.000	175	46	4	39	205	6	178	1.56e-70	213
Msa1170840	MtrunA17_Chr5g0411391	55.488	164	66	3	46	203	44	206	1.70e-60	188
Msa1170840	MtrunA17_Chr8g0347191	52.353	170	73	4	41	205	66	232	9.87e-58	182
Msa1170840	MtrunA17_Chr3g0126471	51.534	163	73	3	44	203	19	178	2.31e-57	179
Msa1170840	MtrunA17_Chr1g0158871	50.909	165	75	2	44	205	17	178	6.83e-57	178
Msa1170840	MtrunA17_Chr1g0151661	52.795	161	69	3	46	202	39	196	1.47e-54	172

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1170840	AT2G40330.1	58.571	210	79	3	1	203	1	209	1.63e-81	242
Msa1170840	AT5G05440.1	60.396	202	74	3	1	200	1	198	7.90e-77	229
Msa1170840	AT2G38310.1	65.285	193	60	3	13	203	8	195	2.27e-73	221
Msa1170840	AT1G01360.1	52.147	163	72	2	46	205	21	180	1.86e-60	187
Msa1170840	AT4G27920.1	50.909	165	76	2	44	205	12	174	1.16e-57	180
Msa1170840	AT4G01026.1	52.201	159	70	3	50	205	27	182	3.58e-56	177
Msa1170840	AT5G53160.2	50.909	165	75	3	44	205	17	178	3.98e-56	176
Msa1170840	AT4G17870.1	50.610	164	77	2	45	205	16	178	3.94e-53	169
Msa1170840	AT2G26040.1	49.383	162	75	3	46	202	22	181	1.39e-50	162
Msa1170840	AT1G73000.1	47.619	168	77	3	46	202	37	204	1.03e-49	160
Msa1170840	AT5G46790.2	51.765	170	75	2	42	205	40	208	9.28e-49	159
Msa1170840	AT5G46790.1	51.765	170	75	2	42	205	40	208	9.28e-49	159
Msa1170840	AT5G45860.1	58.389	149	58	3	59	205	10	156	1.20e-46	151
Msa1170840	AT5G45870.1	56.954	151	62	3	56	205	7	155	2.18e-45	148
Msa1170840	AT4G18620.1	46.914	162	79	2	51	205	1	162	1.23e-35	123
Msa1170840	AT5G53160.1	46.667	105	53	2	44	148	17	118	5.55e-28	103

Find 59 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGGTGGTTTGCAACCGTTTC+TGG	0.080678	8_1:+193269	None:intergenic
CGTGAAGTTCGACTTGTTTC+CGG	0.131980	8_1:-193071	Msa1170840:CDS
AAACGGAGGCCTTGATGGTT+TGG	0.349101	8_1:+193204	None:intergenic
GTGTTGTTGGTGGTGTTCAT+AGG	0.363891	8_1:-192980	Msa1170840:CDS
TCTAACACCGCCATGACTAA+TGG	0.405607	8_1:-193362	Msa1170840:CDS
GTTATTAGTTTCAGTGTTGT+TGG	0.408321	8_1:-192993	Msa1170840:CDS
TTCATAGGTGTAGGAATTAC+CGG	0.411086	8_1:-192965	Msa1170840:CDS
AGGCCTCCGTTTCAACTGTC+TGG	0.430513	8_1:-193193	Msa1170840:CDS
CGACACGCTTGAATTTGTTT+CGG	0.430906	8_1:+193329	None:intergenic
AGTTGAAACGGAGGCCTTGA+TGG	0.442287	8_1:+193199	None:intergenic
TAGGTGTAATTTGCAGTCAC+TGG	0.448044	8_1:-192823	Msa1170840:CDS
GGTGGTTTGCAACCGTTTCT+GGG	0.462922	8_1:+193270	None:intergenic
AACGTAGTTGCCTCCGGTGA+TGG	0.485651	8_1:-193113	Msa1170840:CDS
AACCGTTTCTGTCCCAGAAA+CGG	0.488981	8_1:-193282	Msa1170840:CDS
AGCTGCAACGTAGTTGCCTC+CGG	0.500297	8_1:-193119	Msa1170840:CDS
GCTTGAAACGGCAGGTAGAC+CGG	0.502003	8_1:+193052	None:intergenic
CAACCTCTCCATGCTTGAAA+CGG	0.516613	8_1:+193040	None:intergenic
GGATCACGACGGAGTAGCAC+TGG	0.520709	8_1:+193225	None:intergenic
AACCGTTTCTGGGACAGAAA+CGG	0.521454	8_1:+193280	None:intergenic
GTGACGACGTTGCACGGTGA+TGG	0.524987	8_1:-192939	Msa1170840:CDS
CGTTTCAAGCATGGAGAGGT+TGG	0.529991	8_1:-193039	Msa1170840:CDS
TCGAACCGTTGAAATTGAAC+CGG	0.530260	8_1:+193386	None:intergenic
CGTTGAAATTGAACCGGTGA+AGG	0.532412	8_1:+193392	None:intergenic
ACCGACACGAATGCCATCAC+CGG	0.534492	8_1:+193100	None:intergenic
AACGGACCAGACAGTTGAAA+CGG	0.535986	8_1:+193187	None:intergenic
CAAAATAGAAATGCCTTCAC+CGG	0.537348	8_1:-193405	None:intergenic
CTTCACCGGTTCAATTTCAA+CGG	0.537786	8_1:-193391	Msa1170840:CDS
CTCTCCATGCTTGAAACGGC+AGG	0.544665	8_1:+193044	None:intergenic
ACAAAGTGTTTGTAGCCTTG+TGG	0.546590	8_1:+193146	None:intergenic
TCTACCTGCCGTTTCAAGCA+TGG	0.550915	8_1:-193048	Msa1170840:CDS
CGGTCAGTGACGACGTTGCA+CGG	0.551799	8_1:-192945	Msa1170840:CDS
ATACGTTGTTGATGTACCAC+AGG	0.556769	8_1:-192889	Msa1170840:CDS
TTGGTGGTGTTCATAGGTGT+AGG	0.559793	8_1:-192974	Msa1170840:CDS
ATTAGTTTCAGTGTTGTTGG+TGG	0.561597	8_1:-192990	Msa1170840:CDS
CGTCGTGATCCAAACCATCA+AGG	0.571418	8_1:-193213	Msa1170840:CDS
CGTGCAACGTCGTCACTGAC+CGG	0.576112	8_1:+192946	None:intergenic
CAACACTGAAACTAATAACA+TGG	0.585088	8_1:+192997	None:intergenic
ACAGTTAATGCCATTAGTCA+TGG	0.586782	8_1:+193352	None:intergenic
AGCCTTGTGGATTGTCGAAG+CGG	0.601019	8_1:+193159	None:intergenic
AACCACCACATGCACGTGGT+GGG	0.602296	8_1:-193254	Msa1170840:CDS
ACGTTGCACGGTGATGGAAA+CGG	0.604152	8_1:-192933	Msa1170840:CDS
TCCGGTGATGGCATTCGTGT+CGG	0.606951	8_1:-193101	Msa1170840:CDS
TGTACCACAGGGTAACACTA+AGG	0.609377	8_1:-192877	Msa1170840:CDS
CGCCGCTTCGACAATCCACA+AGG	0.611657	8_1:-193161	Msa1170840:CDS
AAACCACCACATGCACGTGG+TGG	0.622421	8_1:-193255	Msa1170840:CDS
GTCCCACCACGTGCATGTGG+TGG	0.623392	8_1:+193252	None:intergenic
CTGCCGTTTCAAGCATGGAG+AGG	0.630256	8_1:-193043	Msa1170840:CDS
ATTGTCGAAGCGGCGGACAA+CGG	0.630831	8_1:+193169	None:intergenic
CTGCAAATTACACCTAACAA+TGG	0.632688	8_1:+192830	None:intergenic
TGCAAACCACCACATGCACG+TGG	0.642151	8_1:-193258	Msa1170840:CDS
TTTGTCCCACCACGTGCATG+TGG	0.645948	8_1:+193249	None:intergenic
TCTTCCTTAGTGTTACCCTG+TGG	0.656284	8_1:+192873	None:intergenic
GACACGAATGCCATCACCGG+AGG	0.657865	8_1:+193103	None:intergenic
CTTGTGGATTGTCGAAGCGG+CGG	0.689521	8_1:+193162	None:intergenic
CTTGATGGTTTGGATCACGA+CGG	0.697791	8_1:+193214	None:intergenic
GGACCAGACAGTTGAAACGG+AGG	0.712986	8_1:+193190	None:intergenic
TACGTTGTTGATGTACCACA+GGG	0.714710	8_1:-192888	Msa1170840:CDS
TTGCACGGTGATGGAAACGG+AGG	0.721827	8_1:-192930	Msa1170840:CDS
GTTAATGCCATTAGTCATGG+CGG	0.726535	8_1:+193355	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CAACACTGAAACTAATAACA+TGG	+	chr8_1:193201-193220	None:intergenic	30.0%
!	GTTATTAGTTTCAGTGTTGT+TGG	-	chr8_1:193202-193221	Msa1170840:CDS	30.0%
!!	GTTTTGTTGATACCATTGTT+AGG	-	chr8_1:193353-193372	Msa1170840:CDS	30.0%
	ACAGTTAATGCCATTAGTCA+TGG	+	chr8_1:192846-192865	None:intergenic	35.0%
!	ATTAGTTTCAGTGTTGTTGG+TGG	-	chr8_1:193205-193224	Msa1170840:CDS	35.0%
	TTCATAGGTGTAGGAATTAC+CGG	-	chr8_1:193230-193249	Msa1170840:CDS	35.0%
	CTGCAAATTACACCTAACAA+TGG	+	chr8_1:193368-193387	None:intergenic	35.0%
!!	CTTCACCGGTTCAATTTCAA+CGG	-	chr8_1:192804-192823	Msa1170840:CDS	40.0%
	TCGAACCGTTGAAATTGAAC+CGG	+	chr8_1:192812-192831	None:intergenic	40.0%
	GTTAATGCCATTAGTCATGG+CGG	+	chr8_1:192843-192862	None:intergenic	40.0%
!	CGACACGCTTGAATTTGTTT+CGG	+	chr8_1:192869-192888	None:intergenic	40.0%
!	ACAAAGTGTTTGTAGCCTTG+TGG	+	chr8_1:193052-193071	None:intergenic	40.0%
	ATACGTTGTTGATGTACCAC+AGG	-	chr8_1:193306-193325	Msa1170840:CDS	40.0%
	TACGTTGTTGATGTACCACA+GGG	-	chr8_1:193307-193326	Msa1170840:CDS	40.0%
!	TAGGTGTAATTTGCAGTCAC+TGG	-	chr8_1:193372-193391	Msa1170840:CDS	40.0%
	CGTTGAAATTGAACCGGTGA+AGG	+	chr8_1:192806-192825	None:intergenic	45.0%
	TCTAACACCGCCATGACTAA+TGG	-	chr8_1:192833-192852	Msa1170840:CDS	45.0%
	AACCGTTTCTGTCCCAGAAA+CGG	-	chr8_1:192913-192932	Msa1170840:CDS	45.0%
!!	AACCGTTTCTGGGACAGAAA+CGG	+	chr8_1:192918-192937	None:intergenic	45.0%
!	CTTGATGGTTTGGATCACGA+CGG	+	chr8_1:192984-193003	None:intergenic	45.0%
	AACGGACCAGACAGTTGAAA+CGG	+	chr8_1:193011-193030	None:intergenic	45.0%
!	CGTGAAGTTCGACTTGTTTC+CGG	-	chr8_1:193124-193143	Msa1170840:CDS	45.0%
	CAACCTCTCCATGCTTGAAA+CGG	+	chr8_1:193158-193177	None:intergenic	45.0%
!!	GTGTTGTTGGTGGTGTTCAT+AGG	-	chr8_1:193215-193234	Msa1170840:CDS	45.0%
!!	TTGGTGGTGTTCATAGGTGT+AGG	-	chr8_1:193221-193240	Msa1170840:CDS	45.0%
	TGTACCACAGGGTAACACTA+AGG	-	chr8_1:193318-193337	Msa1170840:CDS	45.0%
	TCTTCCTTAGTGTTACCCTG+TGG	+	chr8_1:193325-193344	None:intergenic	45.0%
!	GGTGGTTTGCAACCGTTTCT+GGG	+	chr8_1:192928-192947	None:intergenic	50.0%
!	TGGTGGTTTGCAACCGTTTC+TGG	+	chr8_1:192929-192948	None:intergenic	50.0%
	CGTCGTGATCCAAACCATCA+AGG	-	chr8_1:192982-193001	Msa1170840:CDS	50.0%
!	AAACGGAGGCCTTGATGGTT+TGG	+	chr8_1:192994-193013	None:intergenic	50.0%
	AGTTGAAACGGAGGCCTTGA+TGG	+	chr8_1:192999-193018	None:intergenic	50.0%
!	AGCCTTGTGGATTGTCGAAG+CGG	+	chr8_1:193039-193058	None:intergenic	50.0%
!	TCTACCTGCCGTTTCAAGCA+TGG	-	chr8_1:193147-193166	Msa1170840:CDS	50.0%
	CGTTTCAAGCATGGAGAGGT+TGG	-	chr8_1:193156-193175	Msa1170840:CDS	50.0%
!!	ACGTTGCACGGTGATGGAAA+CGG	-	chr8_1:193262-193281	Msa1170840:CDS	50.0%
	TGCAAACCACCACATGCACG+TGG	-	chr8_1:192937-192956	Msa1170840:CDS	55.0%
	AAACCACCACATGCACGTGG+TGG	-	chr8_1:192940-192959	Msa1170840:CDS	55.0%
	AACCACCACATGCACGTGGT+GGG	-	chr8_1:192941-192960	Msa1170840:CDS	55.0%
	TTTGTCCCACCACGTGCATG+TGG	+	chr8_1:192949-192968	None:intergenic	55.0%
	AGGCCTCCGTTTCAACTGTC+TGG	-	chr8_1:193002-193021	Msa1170840:CDS	55.0%
	GGACCAGACAGTTGAAACGG+AGG	+	chr8_1:193008-193027	None:intergenic	55.0%
	ATTGTCGAAGCGGCGGACAA+CGG	+	chr8_1:193029-193048	None:intergenic	55.0%
!	CTTGTGGATTGTCGAAGCGG+CGG	+	chr8_1:193036-193055	None:intergenic	55.0%
	AGCTGCAACGTAGTTGCCTC+CGG	-	chr8_1:193076-193095	Msa1170840:CDS	55.0%
	AACGTAGTTGCCTCCGGTGA+TGG	-	chr8_1:193082-193101	Msa1170840:CDS	55.0%
!	TCCGGTGATGGCATTCGTGT+CGG	-	chr8_1:193094-193113	Msa1170840:CDS	55.0%
	ACCGACACGAATGCCATCAC+CGG	+	chr8_1:193098-193117	None:intergenic	55.0%
	GCTTGAAACGGCAGGTAGAC+CGG	+	chr8_1:193146-193165	None:intergenic	55.0%
	CTCTCCATGCTTGAAACGGC+AGG	+	chr8_1:193154-193173	None:intergenic	55.0%
!	CTGCCGTTTCAAGCATGGAG+AGG	-	chr8_1:193152-193171	Msa1170840:CDS	55.0%
!!	TTGCACGGTGATGGAAACGG+AGG	-	chr8_1:193265-193284	Msa1170840:CDS	55.0%
	GGATCACGACGGAGTAGCAC+TGG	+	chr8_1:192973-192992	None:intergenic	60.0%
	CGCCGCTTCGACAATCCACA+AGG	-	chr8_1:193034-193053	Msa1170840:CDS	60.0%
	GACACGAATGCCATCACCGG+AGG	+	chr8_1:193095-193114	None:intergenic	60.0%
	CGTGCAACGTCGTCACTGAC+CGG	+	chr8_1:193252-193271	None:intergenic	60.0%
	CGGTCAGTGACGACGTTGCA+CGG	-	chr8_1:193250-193269	Msa1170840:CDS	60.0%
	GTGACGACGTTGCACGGTGA+TGG	-	chr8_1:193256-193275	Msa1170840:CDS	60.0%
	GTCCCACCACGTGCATGTGG+TGG	+	chr8_1:192946-192965	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	192800	193417	192800	ID=Msa1170840;Name=Msa1170840
chr8_1	mRNA	192800	193417	192800	ID=Msa1170840-mRNA-1;Parent=Msa1170840;Name=Msa1170840-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|205
chr8_1	exon	192800	193417	192800	ID=Msa1170840-mRNA-1:exon:43;Parent=Msa1170840-mRNA-1
chr8_1	CDS	192800	193417	192800	ID=Msa1170840-mRNA-1:cds;Parent=Msa1170840-mRNA-1

Gene Sequence

>Msa1170840

ATGCCTTCACCGGTTCAATTTCAACGGTTCGATTCTAACACCGCCATGACTAATGGCATTAACTGTCCGAAACAAATTCAAGCGTGTCGTTACGCGCTTTCTTCTTTAAAATCAACCGTTTCTGTCCCAGAAACGGTTGCAAACCACCACATGCACGTGGTGGGACAAAACCAGTGCTACTCCGTCGTGATCCAAACCATCAAGGCCTCCGTTTCAACTGTCTGGTCCGTTGTCCGCCGCTTCGACAATCCACAAGGCTACAAACACTTTGTTAAAAGCTGCAACGTAGTTGCCTCCGGTGATGGCATTCGTGTCGGTGCTCTTCGTGAAGTTCGACTTGTTTCCGGTCTACCTGCCGTTTCAAGCATGGAGAGGTTGGATATTCTTGATGAAGAACGCCATGTTATTAGTTTCAGTGTTGTTGGTGGTGTTCATAGGTGTAGGAATTACCGGTCAGTGACGACGTTGCACGGTGATGGAAACGGAGGAACAGTTGTCATTGAATCATACGTTGTTGATGTACCACAGGGTAACACTAAGGAAGAAACATGTAGTTTTGTTGATACCATTGTTAGGTGTAATTTGCAGTCACTGGCTCAGATCGCAGAGAAACTGTGA

Protein sequence

>Msa1170840

MPSPVQFQRFDSNTAMTNGINCPKQIQACRYALSSLKSTVSVPETVANHHMHVVGQNQCYSVVIQTIKASVSTVWSVVRRFDNPQGYKHFVKSCNVVASGDGIRVGALREVRLVSGLPAVSSMERLDILDEERHVISFSVVGGVHRCRNYRSVTTLHGDGNGGTVVIESYVVDVPQGNTKEETCSFVDTIVRCNLQSLAQIAEKL*