AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1236980

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1236980 MtrunA17_Chr8g0347621 97.222 72 1 1 1 72 1 71 2.80e-44 136
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1236980 AT3G07568.1 46.575 73 33 2 1 72 1 68 2.97e-19 73.9

Find 35 sgRNAs with CRISPR-Local

Find 257 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTCGTTTGGAAGGATCTT+TGG 0.226923 8_2:+16256570 Msa1236980:CDS
AGGAGTATGATTCTGCAAAT+TGG 0.272002 8_2:-16256669 None:intergenic
GTTTATCAAAATTTACTATT+CGG 0.330684 8_2:+16258269 Msa1236980:three_prime_UTR
TTCTTTCTTCACTCAGTATG+AGG 0.361211 8_2:-16258242 None:intergenic
CAGGTTGAAAGTTAGATATT+CGG 0.363053 8_2:+16258074 Msa1236980:intron
TTTCGTTTGGAAGGATCTTT+GGG 0.373510 8_2:+16256571 Msa1236980:CDS
TTGTTAAACAGAAATAAATA+TGG 0.375111 8_2:-16258375 None:intergenic
AAAGATCCTTCCAAACGAAA+TGG 0.386067 8_2:-16256568 None:intergenic
CTGTTGTTTCCCATTTCGTT+TGG 0.389744 8_2:+16256558 Msa1236980:CDS
AAAGCAGGGTAAGAATTCTA+AGG 0.427254 8_2:-16258195 None:intergenic
CGATTGCTATGGTTCAGCTT+AGG 0.442953 8_2:+16256518 Msa1236980:CDS
TGATCGTGATTTGAAGAAAC+AGG 0.448751 8_2:-16256689 None:intergenic
TGTTTCTTCAAATCACGATC+AGG 0.465250 8_2:+16256691 Msa1236980:CDS
AAGATCCTTCCAAACGAAAT+GGG 0.484074 8_2:-16256567 None:intergenic
TCAGTATGAGGGAATCTCTC+GGG 0.498802 8_2:-16258230 None:intergenic
TGTTTCCCATTTCGTTTGGA+AGG 0.499824 8_2:+16256562 Msa1236980:CDS
GGAAACAACAGCAGCTGAAG+TGG 0.537618 8_2:-16256546 None:intergenic
AAGCTCTGTTGTTCTACGGC+GGG 0.538954 8_2:+16256467 Msa1236980:five_prime_UTR
TCTTTCTTCACTCAGTATGA+GGG 0.551713 8_2:-16258241 None:intergenic
ATTACAATTGTAATTAGTAG+AGG 0.556942 8_2:+16256384 Msa1236980:five_prime_UTR
TGATTCGAACTCGATTGCTA+TGG 0.560773 8_2:+16256507 Msa1236980:CDS
CTCAGTATGAGGGAATCTCT+CGG 0.565270 8_2:-16258231 None:intergenic
TTGGTTCACAATGTTTGTAG+AGG 0.576669 8_2:+16258139 Msa1236980:three_prime_UTR
GGATCGAAGAGCTTTATGCT+TGG 0.577563 8_2:+16258120 Msa1236980:three_prime_UTR
TCACCGTGTTCGTGTGTTGA+CGG 0.582909 8_2:-16256445 None:intergenic
CATAATAAAACAGATTGTTG+AGG 0.600418 8_2:+16258169 Msa1236980:three_prime_UTR
TTACAATTGTAATTAGTAGA+GGG 0.607660 8_2:+16256385 Msa1236980:five_prime_UTR
GAAGCTCTGTTGTTCTACGG+CGG 0.622478 8_2:+16256466 Msa1236980:five_prime_UTR
CCGTGTTCGTGTGTTGACGG+TGG 0.636085 8_2:-16256442 None:intergenic
GGTGAAGCTCTGTTGTTCTA+CGG 0.639334 8_2:+16256463 Msa1236980:five_prime_UTR
CAGTATGAGGGAATCTCTCG+GGG 0.640741 8_2:-16258229 None:intergenic
ATAAAGCTCTTCGATCCTTG+TGG 0.641680 8_2:-16258114 None:intergenic
TGGATTCAGATAACTCGCAC+AGG 0.643307 8_2:-16256422 None:intergenic
CCACCGTCAACACACGAACA+CGG 0.664390 8_2:+16256442 Msa1236980:five_prime_UTR
AAATTGTTGAGTCACGTGAG+AGG 0.677158 8_2:-16256612 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTGATTTAATTGACAATAT+AGG + chr8_2:16256807-16256826 Msa1236980:intron 15.0%
!!! ATCAATAACAATTTCAATTT+TGG - chr8_2:16256840-16256859 None:intergenic 15.0%
!!! TATTGATAATATTATGAAAG+AGG + chr8_2:16256853-16256872 Msa1236980:intron 15.0%
!!! ATGATATTAGTTATTTTGTT+AGG + chr8_2:16256982-16257001 Msa1236980:intron 15.0%
!! GTTTATCAAAATTTACTATT+CGG + chr8_2:16258269-16258288 Msa1236980:three_prime_UTR 15.0%
!! TTGTTAAACAGAAATAAATA+TGG - chr8_2:16258378-16258397 None:intergenic 15.0%
!! ACTAATTACAATTGTAATTA+CGG - chr8_2:16256382-16256401 None:intergenic 15.0%
!!! ATTGATTTAATTGACAATAT+AGG + chr8_2:16256807-16256826 Msa1236980:intron 15.0%
!!! ATCAATAACAATTTCAATTT+TGG - chr8_2:16256840-16256859 None:intergenic 15.0%
!!! TATTGATAATATTATGAAAG+AGG + chr8_2:16256853-16256872 Msa1236980:intron 15.0%
!!! ATGATATTAGTTATTTTGTT+AGG + chr8_2:16256982-16257001 Msa1236980:intron 15.0%
!! GTTTATCAAAATTTACTATT+CGG + chr8_2:16258269-16258288 Msa1236980:three_prime_UTR 15.0%
!! TTGTTAAACAGAAATAAATA+TGG - chr8_2:16258378-16258397 None:intergenic 15.0%
!! ATTACAATTGTAATTAGTAG+AGG + chr8_2:16256384-16256403 Msa1236980:five_prime_UTR 20.0%
!! TTACAATTGTAATTAGTAGA+GGG + chr8_2:16256385-16256404 Msa1236980:five_prime_UTR 20.0%
!! ATGGTTTATTATACCTAAAA+TGG - chr8_2:16256919-16256938 None:intergenic 20.0%
!! TTACATGATGAATTAATCAA+TGG - chr8_2:16257536-16257555 None:intergenic 20.0%
!!! ACTATTTTTTTTTCTACATC+TGG + chr8_2:16257665-16257684 Msa1236980:intron 20.0%
!! ATTACAATTGTAATTAGTAG+AGG + chr8_2:16256384-16256403 Msa1236980:five_prime_UTR 20.0%
!! TTACAATTGTAATTAGTAGA+GGG + chr8_2:16256385-16256404 Msa1236980:five_prime_UTR 20.0%
!! ATGGTTTATTATACCTAAAA+TGG - chr8_2:16256919-16256938 None:intergenic 20.0%
!! TTACATGATGAATTAATCAA+TGG - chr8_2:16257536-16257555 None:intergenic 20.0%
!!! ACTATTTTTTTTTCTACATC+TGG + chr8_2:16257665-16257684 Msa1236980:intron 20.0%
!! ACTCTAAAAATCTTGCTATA+TGG - chr8_2:16256938-16256957 None:intergenic 25.0%
!! TAGGTTTAGTTAGCTTAATT+AGG + chr8_2:16257001-16257020 Msa1236980:intron 25.0%
!! AGGTTTAGTTAGCTTAATTA+GGG + chr8_2:16257002-16257021 Msa1236980:intron 25.0%
! GAGAAAAATAAATTGAGAGA+AGG - chr8_2:16257135-16257154 None:intergenic 25.0%
! AGACTTATTATTGAGAAAGA+CGG - chr8_2:16257172-16257191 None:intergenic 25.0%
! AATAATAAGTCTCTCCAAAT+TGG + chr8_2:16257180-16257199 Msa1236980:intron 25.0%
! TTAGTGGAATTGATATATCA+TGG + chr8_2:16257290-16257309 Msa1236980:intron 25.0%
!! TGATATATCATGGACAATTT+TGG + chr8_2:16257300-16257319 Msa1236980:intron 25.0%
!! GATATATCATGGACAATTTT+GGG + chr8_2:16257301-16257320 Msa1236980:intron 25.0%
!!! AAATGGTTTTGCTAAACATT+AGG + chr8_2:16257491-16257510 Msa1236980:intron 25.0%
! ATGAATTAATCAATGGCATT+TGG - chr8_2:16257529-16257548 None:intergenic 25.0%
!!! TGTTCTTGATAGTTCTAATT+TGG + chr8_2:16258015-16258034 Msa1236980:intron 25.0%
!!! CTCAACAATCTGTTTTATTA+TGG - chr8_2:16258171-16258190 None:intergenic 25.0%
! CATAATAAAACAGATTGTTG+AGG + chr8_2:16258169-16258188 Msa1236980:three_prime_UTR 25.0%
!! AAGCTTTTGTTAACTTCATA+AGG - chr8_2:16258339-16258358 None:intergenic 25.0%
!! ACTCTAAAAATCTTGCTATA+TGG - chr8_2:16256938-16256957 None:intergenic 25.0%
!! TAGGTTTAGTTAGCTTAATT+AGG + chr8_2:16257001-16257020 Msa1236980:intron 25.0%
!! AGGTTTAGTTAGCTTAATTA+GGG + chr8_2:16257002-16257021 Msa1236980:intron 25.0%
! GAGAAAAATAAATTGAGAGA+AGG - chr8_2:16257135-16257154 None:intergenic 25.0%
! AGACTTATTATTGAGAAAGA+CGG - chr8_2:16257172-16257191 None:intergenic 25.0%
! AATAATAAGTCTCTCCAAAT+TGG + chr8_2:16257180-16257199 Msa1236980:intron 25.0%
! TTAGTGGAATTGATATATCA+TGG + chr8_2:16257290-16257309 Msa1236980:intron 25.0%
!! TGATATATCATGGACAATTT+TGG + chr8_2:16257300-16257319 Msa1236980:intron 25.0%
!! GATATATCATGGACAATTTT+GGG + chr8_2:16257301-16257320 Msa1236980:intron 25.0%
!!! AAATGGTTTTGCTAAACATT+AGG + chr8_2:16257491-16257510 Msa1236980:intron 25.0%
! ATGAATTAATCAATGGCATT+TGG - chr8_2:16257529-16257548 None:intergenic 25.0%
!!! TGTTCTTGATAGTTCTAATT+TGG + chr8_2:16258015-16258034 Msa1236980:intron 25.0%
!!! CTCAACAATCTGTTTTATTA+TGG - chr8_2:16258171-16258190 None:intergenic 25.0%
! CATAATAAAACAGATTGTTG+AGG + chr8_2:16258169-16258188 Msa1236980:three_prime_UTR 25.0%
!! AAGCTTTTGTTAACTTCATA+AGG - chr8_2:16258339-16258358 None:intergenic 25.0%
AATAATAAGAATGCAACCCT+AGG - chr8_2:16256759-16256778 None:intergenic 30.0%
!!! GCAAGATTTTTAGAGTTAGT+TGG + chr8_2:16256942-16256961 Msa1236980:intron 30.0%
TGTATCCTACACTAATTGTT+GGG + chr8_2:16257038-16257057 Msa1236980:intron 30.0%
AACTTGATTACAATGAGTGT+AGG - chr8_2:16257082-16257101 None:intergenic 30.0%
ATGAGACTTGCATGATTTAT+TGG + chr8_2:16257206-16257225 Msa1236980:intron 30.0%
!!! TTTTGGGTTTGCATCAATTA+AGG + chr8_2:16257317-16257336 Msa1236980:intron 30.0%
GCATCAATTAAGGTGAATAT+TGG + chr8_2:16257327-16257346 Msa1236980:intron 30.0%
GGAATGTCTGAAAAAACATT+AGG + chr8_2:16257441-16257460 Msa1236980:intron 30.0%
TAGCAAAACCATTTCTAACA+CGG - chr8_2:16257485-16257504 None:intergenic 30.0%
TCATCATGTAATTGCCTAAA+TGG + chr8_2:16257545-16257564 Msa1236980:intron 30.0%
! TCCTATGAATCATGTTCTTT+TGG + chr8_2:16257623-16257642 Msa1236980:intron 30.0%
ACCAAAAGAACATGATTCAT+AGG - chr8_2:16257627-16257646 None:intergenic 30.0%
AACATCTTGTACAAGATCTT+TGG + chr8_2:16257725-16257744 Msa1236980:intron 30.0%
CAGTATGACTAAAAAGAATG+TGG - chr8_2:16257751-16257770 None:intergenic 30.0%
ACATTGAGTACTTAAACATC+TGG + chr8_2:16257792-16257811 Msa1236980:intron 30.0%
AACATCTGGAAATACTAATC+TGG + chr8_2:16257806-16257825 Msa1236980:intron 30.0%
AAAACTAGACTTTACCATAG+AGG + chr8_2:16257858-16257877 Msa1236980:intron 30.0%
AAACTAGACTTTACCATAGA+GGG + chr8_2:16257859-16257878 Msa1236980:intron 30.0%
! AAACCAGTAATGTTTTCTGA+TGG + chr8_2:16257921-16257940 Msa1236980:intron 30.0%
TTACCATCAGAAAACATTAC+TGG - chr8_2:16257927-16257946 None:intergenic 30.0%
!! TGATAGTTCTAATTTGGAAG+TGG + chr8_2:16258021-16258040 Msa1236980:intron 30.0%
! ATGATGTTTTTCCTTCTCTT+TGG + chr8_2:16258051-16258070 Msa1236980:intron 30.0%
! CAGGTTGAAAGTTAGATATT+CGG + chr8_2:16258074-16258093 Msa1236980:intron 30.0%
AATAATAAGAATGCAACCCT+AGG - chr8_2:16256759-16256778 None:intergenic 30.0%
!!! GCAAGATTTTTAGAGTTAGT+TGG + chr8_2:16256942-16256961 Msa1236980:intron 30.0%
TGTATCCTACACTAATTGTT+GGG + chr8_2:16257038-16257057 Msa1236980:intron 30.0%
AACTTGATTACAATGAGTGT+AGG - chr8_2:16257082-16257101 None:intergenic 30.0%
ATGAGACTTGCATGATTTAT+TGG + chr8_2:16257206-16257225 Msa1236980:intron 30.0%
!!! TTTTGGGTTTGCATCAATTA+AGG + chr8_2:16257317-16257336 Msa1236980:intron 30.0%
GCATCAATTAAGGTGAATAT+TGG + chr8_2:16257327-16257346 Msa1236980:intron 30.0%
GGAATGTCTGAAAAAACATT+AGG + chr8_2:16257441-16257460 Msa1236980:intron 30.0%
TAGCAAAACCATTTCTAACA+CGG - chr8_2:16257485-16257504 None:intergenic 30.0%
TCATCATGTAATTGCCTAAA+TGG + chr8_2:16257545-16257564 Msa1236980:intron 30.0%
! TCCTATGAATCATGTTCTTT+TGG + chr8_2:16257623-16257642 Msa1236980:intron 30.0%
ACCAAAAGAACATGATTCAT+AGG - chr8_2:16257627-16257646 None:intergenic 30.0%
AACATCTTGTACAAGATCTT+TGG + chr8_2:16257725-16257744 Msa1236980:intron 30.0%
CAGTATGACTAAAAAGAATG+TGG - chr8_2:16257751-16257770 None:intergenic 30.0%
ACATTGAGTACTTAAACATC+TGG + chr8_2:16257792-16257811 Msa1236980:intron 30.0%
AACATCTGGAAATACTAATC+TGG + chr8_2:16257806-16257825 Msa1236980:intron 30.0%
AAAACTAGACTTTACCATAG+AGG + chr8_2:16257858-16257877 Msa1236980:intron 30.0%
AAACTAGACTTTACCATAGA+GGG + chr8_2:16257859-16257878 Msa1236980:intron 30.0%
! AAACCAGTAATGTTTTCTGA+TGG + chr8_2:16257921-16257940 Msa1236980:intron 30.0%
TTACCATCAGAAAACATTAC+TGG - chr8_2:16257927-16257946 None:intergenic 30.0%
!! TGATAGTTCTAATTTGGAAG+TGG + chr8_2:16258021-16258040 Msa1236980:intron 30.0%
! ATGATGTTTTTCCTTCTCTT+TGG + chr8_2:16258051-16258070 Msa1236980:intron 30.0%
! CAGGTTGAAAGTTAGATATT+CGG + chr8_2:16258074-16258093 Msa1236980:intron 30.0%
AAGATCCTTCCAAACGAAAT+GGG - chr8_2:16256570-16256589 None:intergenic 35.0%
AAAGATCCTTCCAAACGAAA+TGG - chr8_2:16256571-16256590 None:intergenic 35.0%
ATTTCGTTTGGAAGGATCTT+TGG + chr8_2:16256570-16256589 Msa1236980:CDS 35.0%
TTTCGTTTGGAAGGATCTTT+GGG + chr8_2:16256571-16256590 Msa1236980:CDS 35.0%
! AGGAGTATGATTCTGCAAAT+TGG - chr8_2:16256672-16256691 None:intergenic 35.0%
TGATCGTGATTTGAAGAAAC+AGG - chr8_2:16256692-16256711 None:intergenic 35.0%
TGTTTCTTCAAATCACGATC+AGG + chr8_2:16256691-16256710 Msa1236980:CDS 35.0%
! TTAGTGGTTGGTGCAATTAA+GGG - chr8_2:16256722-16256741 None:intergenic 35.0%
! ATTAGTGGTTGGTGCAATTA+AGG - chr8_2:16256723-16256742 None:intergenic 35.0%
TAATTCAACACTACCTTCCT+AGG + chr8_2:16256739-16256758 Msa1236980:intron 35.0%
AATTCAACACTACCTTCCTA+GGG + chr8_2:16256740-16256759 Msa1236980:intron 35.0%
TTAGTTGGAACTGTCAAAGT+TGG + chr8_2:16256957-16256976 Msa1236980:intron 35.0%
CTGTATCCTACACTAATTGT+TGG + chr8_2:16257037-16257056 Msa1236980:intron 35.0%
! GCAAGTCTCATTTTCCAATT+TGG - chr8_2:16257197-16257216 None:intergenic 35.0%
TCAATTCCACTAAATCATGC+TGG - chr8_2:16257283-16257302 None:intergenic 35.0%
TAAGGTGAATATTGGTCAAG+TGG + chr8_2:16257335-16257354 Msa1236980:intron 35.0%
AAGGTGAATATTGGTCAAGT+GGG + chr8_2:16257336-16257355 Msa1236980:intron 35.0%
TCTGAATCCCTATGATTGAA+AGG + chr8_2:16257412-16257431 Msa1236980:intron 35.0%
CACTGTTCCTTTCAATCATA+GGG - chr8_2:16257422-16257441 None:intergenic 35.0%
AAGAAAGACCGTGTTAGAAA+TGG + chr8_2:16257474-16257493 Msa1236980:intron 35.0%
! GATGAAAGTGGTGTCTTTTA+TGG - chr8_2:16257605-16257624 None:intergenic 35.0%
CATGATTCATAGGATGAAAG+TGG - chr8_2:16257617-16257636 None:intergenic 35.0%
TCAAAACGTATCACACTTGA+AGG + chr8_2:16257831-16257850 Msa1236980:intron 35.0%
TTAATATGTGCTACCCTCTA+TGG - chr8_2:16257875-16257894 None:intergenic 35.0%
!! CATTACTGGTTTAGTCTAGA+TGG - chr8_2:16257913-16257932 None:intergenic 35.0%
TGAGAGTTAGAGAGAGAATT+TGG + chr8_2:16257951-16257970 Msa1236980:intron 35.0%
GAGAGTTAGAGAGAGAATTT+GGG + chr8_2:16257952-16257971 Msa1236980:intron 35.0%
TTGGTTCACAATGTTTGTAG+AGG + chr8_2:16258139-16258158 Msa1236980:three_prime_UTR 35.0%
AAAGCAGGGTAAGAATTCTA+AGG - chr8_2:16258198-16258217 None:intergenic 35.0%
TCTTTCTTCACTCAGTATGA+GGG - chr8_2:16258244-16258263 None:intergenic 35.0%
TTCTTTCTTCACTCAGTATG+AGG - chr8_2:16258245-16258264 None:intergenic 35.0%
AAGATCCTTCCAAACGAAAT+GGG - chr8_2:16256570-16256589 None:intergenic 35.0%
AAAGATCCTTCCAAACGAAA+TGG - chr8_2:16256571-16256590 None:intergenic 35.0%
ATTTCGTTTGGAAGGATCTT+TGG + chr8_2:16256570-16256589 Msa1236980:CDS 35.0%
TTTCGTTTGGAAGGATCTTT+GGG + chr8_2:16256571-16256590 Msa1236980:CDS 35.0%
! AGGAGTATGATTCTGCAAAT+TGG - chr8_2:16256672-16256691 None:intergenic 35.0%
TGATCGTGATTTGAAGAAAC+AGG - chr8_2:16256692-16256711 None:intergenic 35.0%
TGTTTCTTCAAATCACGATC+AGG + chr8_2:16256691-16256710 Msa1236980:CDS 35.0%
! TTAGTGGTTGGTGCAATTAA+GGG - chr8_2:16256722-16256741 None:intergenic 35.0%
! ATTAGTGGTTGGTGCAATTA+AGG - chr8_2:16256723-16256742 None:intergenic 35.0%
TAATTCAACACTACCTTCCT+AGG + chr8_2:16256739-16256758 Msa1236980:intron 35.0%
AATTCAACACTACCTTCCTA+GGG + chr8_2:16256740-16256759 Msa1236980:intron 35.0%
TTAGTTGGAACTGTCAAAGT+TGG + chr8_2:16256957-16256976 Msa1236980:intron 35.0%
CTGTATCCTACACTAATTGT+TGG + chr8_2:16257037-16257056 Msa1236980:intron 35.0%
! GCAAGTCTCATTTTCCAATT+TGG - chr8_2:16257197-16257216 None:intergenic 35.0%
TCAATTCCACTAAATCATGC+TGG - chr8_2:16257283-16257302 None:intergenic 35.0%
TAAGGTGAATATTGGTCAAG+TGG + chr8_2:16257335-16257354 Msa1236980:intron 35.0%
AAGGTGAATATTGGTCAAGT+GGG + chr8_2:16257336-16257355 Msa1236980:intron 35.0%
TCTGAATCCCTATGATTGAA+AGG + chr8_2:16257412-16257431 Msa1236980:intron 35.0%
CACTGTTCCTTTCAATCATA+GGG - chr8_2:16257422-16257441 None:intergenic 35.0%
AAGAAAGACCGTGTTAGAAA+TGG + chr8_2:16257474-16257493 Msa1236980:intron 35.0%
! GATGAAAGTGGTGTCTTTTA+TGG - chr8_2:16257605-16257624 None:intergenic 35.0%
CATGATTCATAGGATGAAAG+TGG - chr8_2:16257617-16257636 None:intergenic 35.0%
TCAAAACGTATCACACTTGA+AGG + chr8_2:16257831-16257850 Msa1236980:intron 35.0%
TTAATATGTGCTACCCTCTA+TGG - chr8_2:16257875-16257894 None:intergenic 35.0%
!! CATTACTGGTTTAGTCTAGA+TGG - chr8_2:16257913-16257932 None:intergenic 35.0%
TGAGAGTTAGAGAGAGAATT+TGG + chr8_2:16257951-16257970 Msa1236980:intron 35.0%
GAGAGTTAGAGAGAGAATTT+GGG + chr8_2:16257952-16257971 Msa1236980:intron 35.0%
TTGGTTCACAATGTTTGTAG+AGG + chr8_2:16258139-16258158 Msa1236980:three_prime_UTR 35.0%
AAAGCAGGGTAAGAATTCTA+AGG - chr8_2:16258198-16258217 None:intergenic 35.0%
TCTTTCTTCACTCAGTATGA+GGG - chr8_2:16258244-16258263 None:intergenic 35.0%
TTCTTTCTTCACTCAGTATG+AGG - chr8_2:16258245-16258264 None:intergenic 35.0%
TGATTCGAACTCGATTGCTA+TGG + chr8_2:16256507-16256526 Msa1236980:CDS 40.0%
!! CTGTTGTTTCCCATTTCGTT+TGG + chr8_2:16256558-16256577 Msa1236980:CDS 40.0%
TGTTTCCCATTTCGTTTGGA+AGG + chr8_2:16256562-16256581 Msa1236980:CDS 40.0%
AAATTGTTGAGTCACGTGAG+AGG - chr8_2:16256615-16256634 None:intergenic 40.0%
! GGTAGTGTTGAATTAGTGGT+TGG - chr8_2:16256734-16256753 None:intergenic 40.0%
! GGAAGGTAGTGTTGAATTAG+TGG - chr8_2:16256738-16256757 None:intergenic 40.0%
ATAAGAATGCAACCCTAGGA+AGG - chr8_2:16256755-16256774 None:intergenic 40.0%
! CATGCGAACTCTACCATTTT+AGG + chr8_2:16256903-16256922 Msa1236980:intron 40.0%
AGAGACCCAACAATTAGTGT+AGG - chr8_2:16257046-16257065 None:intergenic 40.0%
ATGAGACCAGCATGATTTAG+TGG + chr8_2:16257274-16257293 Msa1236980:intron 40.0%
CCACTGTTCCTTTCAATCAT+AGG - chr8_2:16257423-16257442 None:intergenic 40.0%
CCTATGATTGAAAGGAACAG+TGG + chr8_2:16257420-16257439 Msa1236980:intron 40.0%
TAGAGAGAGAATTTGGGAGA+TGG + chr8_2:16257958-16257977 Msa1236980:intron 40.0%
! TGTTTTTCCTTCTCTTTGGC+AGG + chr8_2:16258055-16258074 Msa1236980:intron 40.0%
ATAAAGCTCTTCGATCCTTG+TGG - chr8_2:16258117-16258136 None:intergenic 40.0%
GGGAGACAGATAAAAAAGCA+GGG - chr8_2:16258212-16258231 None:intergenic 40.0%
TGATTCGAACTCGATTGCTA+TGG + chr8_2:16256507-16256526 Msa1236980:CDS 40.0%
!! CTGTTGTTTCCCATTTCGTT+TGG + chr8_2:16256558-16256577 Msa1236980:CDS 40.0%
TGTTTCCCATTTCGTTTGGA+AGG + chr8_2:16256562-16256581 Msa1236980:CDS 40.0%
AAATTGTTGAGTCACGTGAG+AGG - chr8_2:16256615-16256634 None:intergenic 40.0%
! GGTAGTGTTGAATTAGTGGT+TGG - chr8_2:16256734-16256753 None:intergenic 40.0%
! GGAAGGTAGTGTTGAATTAG+TGG - chr8_2:16256738-16256757 None:intergenic 40.0%
ATAAGAATGCAACCCTAGGA+AGG - chr8_2:16256755-16256774 None:intergenic 40.0%
! CATGCGAACTCTACCATTTT+AGG + chr8_2:16256903-16256922 Msa1236980:intron 40.0%
AGAGACCCAACAATTAGTGT+AGG - chr8_2:16257046-16257065 None:intergenic 40.0%
ATGAGACCAGCATGATTTAG+TGG + chr8_2:16257274-16257293 Msa1236980:intron 40.0%
CCACTGTTCCTTTCAATCAT+AGG - chr8_2:16257423-16257442 None:intergenic 40.0%
CCTATGATTGAAAGGAACAG+TGG + chr8_2:16257420-16257439 Msa1236980:intron 40.0%
TAGAGAGAGAATTTGGGAGA+TGG + chr8_2:16257958-16257977 Msa1236980:intron 40.0%
! TGTTTTTCCTTCTCTTTGGC+AGG + chr8_2:16258055-16258074 Msa1236980:intron 40.0%
ATAAAGCTCTTCGATCCTTG+TGG - chr8_2:16258117-16258136 None:intergenic 40.0%
GGGAGACAGATAAAAAAGCA+GGG - chr8_2:16258212-16258231 None:intergenic 40.0%
TGGATTCAGATAACTCGCAC+AGG - chr8_2:16256425-16256444 None:intergenic 45.0%
GGTGAAGCTCTGTTGTTCTA+CGG + chr8_2:16256463-16256482 Msa1236980:five_prime_UTR 45.0%
CGATTGCTATGGTTCAGCTT+AGG + chr8_2:16256518-16256537 Msa1236980:CDS 45.0%
TGGTCAAGTGGGAAGATAAC+TGG + chr8_2:16257347-16257366 Msa1236980:intron 45.0%
GGTCAAGTGGGAAGATAACT+GGG + chr8_2:16257348-16257367 Msa1236980:intron 45.0%
! CTGGGTTCTGGTGAAAAAAG+TGG + chr8_2:16257366-16257385 Msa1236980:intron 45.0%
TTCCATTAGGTGGGCCATTT+AGG - chr8_2:16257562-16257581 None:intergenic 45.0%
TAAATGGCCCACCTAATGGA+AGG + chr8_2:16257561-16257580 Msa1236980:intron 45.0%
AAATGGCCCACCTAATGGAA+GGG + chr8_2:16257562-16257581 Msa1236980:intron 45.0%
CTTATGCCCTTCCATTAGGT+GGG - chr8_2:16257571-16257590 None:intergenic 45.0%
! AAGCCTTATGCCCTTCCATT+AGG - chr8_2:16257575-16257594 None:intergenic 45.0%
CTTTCAACCTGCCAAAGAGA+AGG - chr8_2:16258065-16258084 None:intergenic 45.0%
! CCTTGTGGAAAACGTTTTGC+TGG - chr8_2:16258102-16258121 None:intergenic 45.0%
! CCAGCAAAACGTTTTCCACA+AGG + chr8_2:16258099-16258118 Msa1236980:three_prime_UTR 45.0%
! GGATCGAAGAGCTTTATGCT+TGG + chr8_2:16258120-16258139 Msa1236980:three_prime_UTR 45.0%
GGGGAGACAGATAAAAAAGC+AGG - chr8_2:16258213-16258232 None:intergenic 45.0%
TCAGTATGAGGGAATCTCTC+GGG - chr8_2:16258233-16258252 None:intergenic 45.0%
CTCAGTATGAGGGAATCTCT+CGG - chr8_2:16258234-16258253 None:intergenic 45.0%
TGGATTCAGATAACTCGCAC+AGG - chr8_2:16256425-16256444 None:intergenic 45.0%
GGTGAAGCTCTGTTGTTCTA+CGG + chr8_2:16256463-16256482 Msa1236980:five_prime_UTR 45.0%
CGATTGCTATGGTTCAGCTT+AGG + chr8_2:16256518-16256537 Msa1236980:CDS 45.0%
TGGTCAAGTGGGAAGATAAC+TGG + chr8_2:16257347-16257366 Msa1236980:intron 45.0%
GGTCAAGTGGGAAGATAACT+GGG + chr8_2:16257348-16257367 Msa1236980:intron 45.0%
! CTGGGTTCTGGTGAAAAAAG+TGG + chr8_2:16257366-16257385 Msa1236980:intron 45.0%
TTCCATTAGGTGGGCCATTT+AGG - chr8_2:16257562-16257581 None:intergenic 45.0%
TAAATGGCCCACCTAATGGA+AGG + chr8_2:16257561-16257580 Msa1236980:intron 45.0%
AAATGGCCCACCTAATGGAA+GGG + chr8_2:16257562-16257581 Msa1236980:intron 45.0%
CTTATGCCCTTCCATTAGGT+GGG - chr8_2:16257571-16257590 None:intergenic 45.0%
! AAGCCTTATGCCCTTCCATT+AGG - chr8_2:16257575-16257594 None:intergenic 45.0%
CTTTCAACCTGCCAAAGAGA+AGG - chr8_2:16258065-16258084 None:intergenic 45.0%
! CCTTGTGGAAAACGTTTTGC+TGG - chr8_2:16258102-16258121 None:intergenic 45.0%
! CCAGCAAAACGTTTTCCACA+AGG + chr8_2:16258099-16258118 Msa1236980:three_prime_UTR 45.0%
! GGATCGAAGAGCTTTATGCT+TGG + chr8_2:16258120-16258139 Msa1236980:three_prime_UTR 45.0%
GGGGAGACAGATAAAAAAGC+AGG - chr8_2:16258213-16258232 None:intergenic 45.0%
TCAGTATGAGGGAATCTCTC+GGG - chr8_2:16258233-16258252 None:intergenic 45.0%
CTCAGTATGAGGGAATCTCT+CGG - chr8_2:16258234-16258253 None:intergenic 45.0%
!! TGTAAAATAAAAATATTAAA+GGG - chr8_2:16257775-16257794 None:intergenic 5.0%
!! ATGTAAAATAAAAATATTAA+AGG - chr8_2:16257776-16257795 None:intergenic 5.0%
!! AAATGAAAAAAATTAATATT+TGG - chr8_2:16258300-16258319 None:intergenic 5.0%
!! TGTAAAATAAAAATATTAAA+GGG - chr8_2:16257775-16257794 None:intergenic 5.0%
!! ATGTAAAATAAAAATATTAA+AGG - chr8_2:16257776-16257795 None:intergenic 5.0%
!! AAATGAAAAAAATTAATATT+TGG - chr8_2:16258300-16258319 None:intergenic 5.0%
TCACCGTGTTCGTGTGTTGA+CGG - chr8_2:16256448-16256467 None:intergenic 50.0%
GAAGCTCTGTTGTTCTACGG+CGG + chr8_2:16256466-16256485 Msa1236980:five_prime_UTR 50.0%
AAGCTCTGTTGTTCTACGGC+GGG + chr8_2:16256467-16256486 Msa1236980:five_prime_UTR 50.0%
GGAAACAACAGCAGCTGAAG+TGG - chr8_2:16256549-16256568 None:intergenic 50.0%
GTGGGAAGATAACTGGGTTC+TGG + chr8_2:16257354-16257373 Msa1236980:intron 50.0%
TGCCTAAATGGCCCACCTAA+TGG + chr8_2:16257557-16257576 Msa1236980:intron 50.0%
CCTTATGCCCTTCCATTAGG+TGG - chr8_2:16257572-16257591 None:intergenic 50.0%
! CCACCTAATGGAAGGGCATA+AGG + chr8_2:16257569-16257588 Msa1236980:intron 50.0%
CAGTATGAGGGAATCTCTCG+GGG - chr8_2:16258232-16258251 None:intergenic 50.0%
TCACCGTGTTCGTGTGTTGA+CGG - chr8_2:16256448-16256467 None:intergenic 50.0%
GAAGCTCTGTTGTTCTACGG+CGG + chr8_2:16256466-16256485 Msa1236980:five_prime_UTR 50.0%
AAGCTCTGTTGTTCTACGGC+GGG + chr8_2:16256467-16256486 Msa1236980:five_prime_UTR 50.0%
GGAAACAACAGCAGCTGAAG+TGG - chr8_2:16256549-16256568 None:intergenic 50.0%
GTGGGAAGATAACTGGGTTC+TGG + chr8_2:16257354-16257373 Msa1236980:intron 50.0%
TGCCTAAATGGCCCACCTAA+TGG + chr8_2:16257557-16257576 Msa1236980:intron 50.0%
CCTTATGCCCTTCCATTAGG+TGG - chr8_2:16257572-16257591 None:intergenic 50.0%
! CCACCTAATGGAAGGGCATA+AGG + chr8_2:16257569-16257588 Msa1236980:intron 50.0%
CAGTATGAGGGAATCTCTCG+GGG - chr8_2:16258232-16258251 None:intergenic 50.0%
CCACCGTCAACACACGAACA+CGG + chr8_2:16256442-16256461 Msa1236980:five_prime_UTR 55.0%
CCACCGTCAACACACGAACA+CGG + chr8_2:16256442-16256461 Msa1236980:five_prime_UTR 55.0%
! CCGTGTTCGTGTGTTGACGG+TGG - chr8_2:16256445-16256464 None:intergenic 60.0%
! CCGTGTTCGTGTGTTGACGG+TGG - chr8_2:16256445-16256464 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 16256381 16258459 16256381 ID=Msa1236980;Name=Msa1236980
chr8_2 mRNA 16256381 16258459 16256381 ID=Msa1236980-mRNA-1;Parent=Msa1236980;Name=Msa1236980-mRNA-1;_AED=0.02;_eAED=0.02;_QI=125|1|1|1|1|1|2|371|72
chr8_2 exon 16256381 16256712 16256381 ID=Msa1236980-mRNA-1:exon:6048;Parent=Msa1236980-mRNA-1
chr8_2 exon 16258077 16258459 16258077 ID=Msa1236980-mRNA-1:exon:6049;Parent=Msa1236980-mRNA-1
chr8_2 five_prime_UTR 16256381 16256505 16256381 ID=Msa1236980-mRNA-1:five_prime_utr;Parent=Msa1236980-mRNA-1
chr8_2 CDS 16256506 16256712 16256506 ID=Msa1236980-mRNA-1:cds;Parent=Msa1236980-mRNA-1
chr8_2 CDS 16258077 16258088 16258077 ID=Msa1236980-mRNA-1:cds;Parent=Msa1236980-mRNA-1
chr8_2 three_prime_UTR 16258089 16258459 16258089 ID=Msa1236980-mRNA-1:three_prime_utr;Parent=Msa1236980-mRNA-1
Gene Sequence

>Msa1236980

ATGATTCGAACTCGATTGCTATGGTTCAGCTTAGGATTCACCACTTCAGCTGCTGTTGTTTCCCATTTCGTTTGGAAGGATCTTTGGGTTGATCGTCACGCTTTATCCTCTCACGTGACTCAACAATTTGACGCTCTTCAAACTCGAATCTCAATTCTTGAATCCAATTTGCAGAATCATACTCCTGTTTCTTCAAATCACGATCAGGTTGAAAGTTAG
Protein sequence

>Msa1236980

MIRTRLLWFSLGFTTSAAVVSHFVWKDLWVDRHALSSHVTQQFDALQTRISILESNLQNHTPVSSNHDQVES*