AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0077320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0077320 MtrunA17_Chr1g0186541 93.976 166 4 1 1 166 1 160 5.84e-109 307
Msa0077320 MtrunA17_Chr1g0184251 51.266 158 68 2 1 158 1 149 6.28e-46 147
Msa0077320 MtrunA17_Chr7g0261051 51.613 155 66 2 1 155 1 146 4.51e-45 145
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0077320 AT5G43460.2 62.048 166 49 4 1 165 1 153 3.60e-62 189
Msa0077320 AT5G43460.1 62.048 166 49 4 1 165 1 153 3.60e-62 189
Msa0077320 AT1G04340.1 56.287 167 62 3 1 165 1 158 3.50e-58 179
Msa0077320 AT4G14420.1 49.398 166 74 3 1 165 1 157 1.21e-40 134
Msa0077320 AT3G23180.1 29.752 121 63 2 7 127 24 122 5.85e-12 62.0
Msa0077320 AT3G23180.2 29.752 121 63 2 7 127 24 122 5.85e-12 62.0
Msa0077320 AT3G23190.1 34.677 124 55 2 8 131 25 122 1.65e-11 60.5

Find 54 sgRNAs with CRISPR-Local

Find 241 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATATAATATTTGATTTAAC+TGG 0.229790 1_2:-54205589 None:intergenic
CCTTCCCATTGGTCAGATTA+AGG 0.238405 1_2:+54203438 Msa0077320:five_prime_UTR
TAATCGGCACAATTTGATTA+TGG 0.247158 1_2:+54205428 Msa0077320:three_prime_UTR
TGTAGAAATCATACAGAATA+GGG 0.299080 1_2:-54205082 None:intergenic
GTTGCTAAATGAGTTCATTC+AGG 0.313701 1_2:+54205136 Msa0077320:CDS
AATGGGGTTCTTCTCCTTCA+TGG 0.331747 1_2:+54203578 Msa0077320:exon
ACATAAGTTGCGTTAAAATC+TGG 0.340201 1_2:-54204713 None:intergenic
ATCAAATATTATATGATGTC+CGG 0.350936 1_2:+54205597 Msa0077320:three_prime_UTR
TTTGTGTTTGGCAGCACGTT+TGG 0.363466 1_2:+54204884 Msa0077320:CDS
TTTAGCAACAAACCGTATTC+CGG 0.367251 1_2:-54205122 None:intergenic
GGGAATCAATTCCTTTGAAA+AGG 0.377676 1_2:-54204639 None:intergenic
TTCCTCAAAATCGTGAGCTT+GGG 0.400618 1_2:-54204659 None:intergenic
GGAGGAATTCTATTTGTGTT+TGG 0.403655 1_2:+54204872 Msa0077320:CDS
ATTGTTGTGTTGCTATGTTT+TGG 0.404036 1_2:+54205390 Msa0077320:three_prime_UTR
TTTCCTCAAAATCGTGAGCT+TGG 0.406891 1_2:-54204660 None:intergenic
ATCATATTTCTCAAGGGAGT+TGG 0.422933 1_2:+54204851 Msa0077320:CDS
TCAAATATTATATGATGTCC+GGG 0.433479 1_2:+54205598 Msa0077320:three_prime_UTR
ACTTATGTAGCTTTCACATC+TGG 0.433495 1_2:+54205049 Msa0077320:intron
TTGTAGAAATCATACAGAAT+AGG 0.434912 1_2:-54205083 None:intergenic
TGTGTCTGTTTACTAAGAAC+AGG 0.440592 1_2:+54205360 Msa0077320:three_prime_UTR
GGAATCAATTCCTTTGAAAA+GGG 0.450002 1_2:-54204638 None:intergenic
CACGTTTGGATCCTTTCTCT+TGG 0.450868 1_2:+54204898 Msa0077320:CDS
GGCACAATTTGATTATGGAT+TGG 0.463449 1_2:+54205433 Msa0077320:three_prime_UTR
ATGGGGTTCTTCTCCTTCAT+GGG 0.477665 1_2:+54203579 Msa0077320:CDS
CTCTCCTTAATCTGACCAAT+GGG 0.478163 1_2:-54203442 None:intergenic
AAACCGTATTCCGGCTTAGT+AGG 0.480706 1_2:-54205113 None:intergenic
TGCCACTATCATATTTCTCA+AGG 0.486755 1_2:+54204844 Msa0077320:CDS
ATAGAATTTATGTTGTTGCT+AGG 0.490103 1_2:+54204588 Msa0077320:intron
AAAATTTATCCTCCAAATTG+GGG 0.503296 1_2:+54204681 Msa0077320:CDS
TCCCTTGAGAAATATGATAG+TGG 0.507226 1_2:-54204846 None:intergenic
ATAATTTGGTTCCTTCCCAT+TGG 0.507694 1_2:+54203427 None:intergenic
GGAAGCATTTACCAAGAGAA+AGG 0.510396 1_2:-54204909 None:intergenic
TTTAATGAATTTGATGCCAC+TGG 0.511159 1_2:+54204611 Msa0077320:CDS
ATATTTCTCAAGGGAGTTGG+AGG 0.516522 1_2:+54204854 Msa0077320:CDS
CCCTCTTCATCCTCTCCGCA+TGG 0.518155 1_2:+54203619 Msa0077320:CDS
AAAGAGAACACGGCCCATGA+AGG 0.535203 1_2:-54203592 None:intergenic
GAACTGAATAAAACATTGCA+AGG 0.549559 1_2:-54205543 None:intergenic
AGACCTACTAAGCCGGAATA+CGG 0.551495 1_2:+54205110 Msa0077320:CDS
TTACGATTCTGTTGTACTTG+CGG 0.554679 1_2:+54205474 Msa0077320:three_prime_UTR
CAACTATAGACCTACTAAGC+CGG 0.570920 1_2:+54205103 Msa0077320:CDS
GCCATGCGGAGAGGATGAAG+AGG 0.600238 1_2:-54203620 None:intergenic
GCCACTATCATATTTCTCAA+GGG 0.605942 1_2:+54204845 Msa0077320:CDS
AATGAATTTGATGCCACTGG+TGG 0.607390 1_2:+54204614 Msa0077320:CDS
CCTTAATCTGACCAATGGGA+AGG 0.608374 1_2:-54203438 None:intergenic
ACTCTCCTTAATCTGACCAA+TGG 0.608784 1_2:-54203443 None:intergenic
AGAGGGAAGCAAAGAGAACA+CGG 0.609383 1_2:-54203602 None:intergenic
CTTTGAAAAGGGTCCACCAG+TGG 0.612462 1_2:-54204627 None:intergenic
CAATTCCCAGAAAACAGTTG+AGG 0.642642 1_2:+54205293 Msa0077320:CDS
CCATGCGGAGAGGATGAAGA+GGG 0.643032 1_2:-54203619 None:intergenic
GTAGAAATCATACAGAATAG+GGG 0.645008 1_2:-54205081 None:intergenic
CTGACATCTGCCATGCGGAG+AGG 0.666177 1_2:-54203629 None:intergenic
CAAATATTATATGATGTCCG+GGG 0.676792 1_2:+54205599 Msa0077320:three_prime_UTR
AAATTTATCCTCCAAATTGG+GGG 0.685692 1_2:+54204682 Msa0077320:CDS
GAGAACTGACATCTGCCATG+CGG 0.716281 1_2:-54203634 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAATTTCAAGATTTCAT+TGG - chr1_2:54203985-54204004 None:intergenic 15.0%
!!! CATATAATATTTGATTTAAC+TGG - chr1_2:54205592-54205611 None:intergenic 15.0%
!! TTAAAATTTCAAGATTTCAT+TGG - chr1_2:54203985-54204004 None:intergenic 15.0%
!!! CATATAATATTTGATTTAAC+TGG - chr1_2:54205592-54205611 None:intergenic 15.0%
!! TTAAATTATGCTTTACTTGT+TGG + chr1_2:54203895-54203914 Msa0077320:intron 20.0%
!! TAAATTATGCTTTACTTGTT+GGG + chr1_2:54203896-54203915 Msa0077320:intron 20.0%
!! ATTGAAAAAAGCAACTTAAA+AGG - chr1_2:54204030-54204049 None:intergenic 20.0%
!!! TTGCTTTTTTCAATTTATTG+AGG + chr1_2:54204036-54204055 Msa0077320:intron 20.0%
!!! ATTTGTTACATAGATTTGTT+AGG + chr1_2:54204144-54204163 Msa0077320:intron 20.0%
!!! TTTGTTACATAGATTTGTTA+GGG + chr1_2:54204145-54204164 Msa0077320:intron 20.0%
!! AAAAATTTATCCTCCAAATT+GGG + chr1_2:54204680-54204699 Msa0077320:CDS 20.0%
!!! TTCATATTTTGATATTCTTC+TGG + chr1_2:54204986-54205005 Msa0077320:intron 20.0%
!!! TCTAATAATGTGTTTACTTT+TGG + chr1_2:54205215-54205234 Msa0077320:intron 20.0%
!! ATCAAATATTATATGATGTC+CGG + chr1_2:54205597-54205616 Msa0077320:three_prime_UTR 20.0%
!! TTAAATTATGCTTTACTTGT+TGG + chr1_2:54203895-54203914 Msa0077320:intron 20.0%
!! TAAATTATGCTTTACTTGTT+GGG + chr1_2:54203896-54203915 Msa0077320:intron 20.0%
!! ATTGAAAAAAGCAACTTAAA+AGG - chr1_2:54204030-54204049 None:intergenic 20.0%
!!! TTGCTTTTTTCAATTTATTG+AGG + chr1_2:54204036-54204055 Msa0077320:intron 20.0%
!!! ATTTGTTACATAGATTTGTT+AGG + chr1_2:54204144-54204163 Msa0077320:intron 20.0%
!!! TTTGTTACATAGATTTGTTA+GGG + chr1_2:54204145-54204164 Msa0077320:intron 20.0%
!! AAAAATTTATCCTCCAAATT+GGG + chr1_2:54204680-54204699 Msa0077320:CDS 20.0%
!!! TTCATATTTTGATATTCTTC+TGG + chr1_2:54204986-54205005 Msa0077320:intron 20.0%
!!! TCTAATAATGTGTTTACTTT+TGG + chr1_2:54205215-54205234 Msa0077320:intron 20.0%
!! ATCAAATATTATATGATGTC+CGG + chr1_2:54205597-54205616 Msa0077320:three_prime_UTR 20.0%
! TAGAGAGAGAAAACAAAAAT+GGG + chr1_2:54203561-54203580 Msa0077320:exon 25.0%
!!! AGTAGCAATTTTGAAAAGAA+TGG - chr1_2:54203702-54203721 None:intergenic 25.0%
! GCATAAAAACGGAAATAAAA+CGG - chr1_2:54203737-54203756 None:intergenic 25.0%
!!! TTTAACTGTGTTTTGAAATC+TGG + chr1_2:54204085-54204104 Msa0077320:intron 25.0%
!! TATTATCCTAGAGAGTTTTA+TGG + chr1_2:54204174-54204193 Msa0077320:intron 25.0%
! TTATTTCCATAAAACTCTCT+AGG - chr1_2:54204183-54204202 None:intergenic 25.0%
! ATTGCTATGATATAATGTCT+TGG + chr1_2:54204356-54204375 Msa0077320:intron 25.0%
!! AACAAAAGTATATGCTACAT+AGG - chr1_2:54204506-54204525 None:intergenic 25.0%
! ATAGAATTTATGTTGTTGCT+AGG + chr1_2:54204588-54204607 Msa0077320:intron 25.0%
! GAAAAATTTATCCTCCAAAT+TGG + chr1_2:54204679-54204698 Msa0077320:CDS 25.0%
! AAAATTTATCCTCCAAATTG+GGG + chr1_2:54204681-54204700 Msa0077320:CDS 25.0%
! GAATGAATAGACATAGATAT+TGG + chr1_2:54204945-54204964 Msa0077320:intron 25.0%
! TGTAGAAATCATACAGAATA+GGG - chr1_2:54205085-54205104 None:intergenic 25.0%
! TTGTAGAAATCATACAGAAT+AGG - chr1_2:54205086-54205105 None:intergenic 25.0%
!!! GAATGGTTGTTTTTGTTAAT+CGG + chr1_2:54205412-54205431 Msa0077320:three_prime_UTR 25.0%
! TCAAATATTATATGATGTCC+GGG + chr1_2:54205598-54205617 Msa0077320:three_prime_UTR 25.0%
! TAGAGAGAGAAAACAAAAAT+GGG + chr1_2:54203561-54203580 Msa0077320:exon 25.0%
!!! AGTAGCAATTTTGAAAAGAA+TGG - chr1_2:54203702-54203721 None:intergenic 25.0%
! GCATAAAAACGGAAATAAAA+CGG - chr1_2:54203737-54203756 None:intergenic 25.0%
!!! TTTAACTGTGTTTTGAAATC+TGG + chr1_2:54204085-54204104 Msa0077320:intron 25.0%
!! TATTATCCTAGAGAGTTTTA+TGG + chr1_2:54204174-54204193 Msa0077320:intron 25.0%
! TTATTTCCATAAAACTCTCT+AGG - chr1_2:54204183-54204202 None:intergenic 25.0%
! ATTGCTATGATATAATGTCT+TGG + chr1_2:54204356-54204375 Msa0077320:intron 25.0%
!! AACAAAAGTATATGCTACAT+AGG - chr1_2:54204506-54204525 None:intergenic 25.0%
! ATAGAATTTATGTTGTTGCT+AGG + chr1_2:54204588-54204607 Msa0077320:intron 25.0%
! GAAAAATTTATCCTCCAAAT+TGG + chr1_2:54204679-54204698 Msa0077320:CDS 25.0%
! AAAATTTATCCTCCAAATTG+GGG + chr1_2:54204681-54204700 Msa0077320:CDS 25.0%
! GAATGAATAGACATAGATAT+TGG + chr1_2:54204945-54204964 Msa0077320:intron 25.0%
! TGTAGAAATCATACAGAATA+GGG - chr1_2:54205085-54205104 None:intergenic 25.0%
! TTGTAGAAATCATACAGAAT+AGG - chr1_2:54205086-54205105 None:intergenic 25.0%
!!! GAATGGTTGTTTTTGTTAAT+CGG + chr1_2:54205412-54205431 Msa0077320:three_prime_UTR 25.0%
CTAGAGAGAGAAAACAAAAA+TGG + chr1_2:54203560-54203579 Msa0077320:exon 30.0%
AGAGAGAGAAAACAAAAATG+GGG + chr1_2:54203562-54203581 Msa0077320:exon 30.0%
!! TATTTCCGTTTTTATGCATC+TGG + chr1_2:54203740-54203759 Msa0077320:intron 30.0%
!! ATTTCCGTTTTTATGCATCT+GGG + chr1_2:54203741-54203760 Msa0077320:intron 30.0%
AAAACCCAGATGCATAAAAA+CGG - chr1_2:54203748-54203767 None:intergenic 30.0%
! TTTTCACCAGATTTGGATTT+TGG + chr1_2:54203764-54203783 Msa0077320:intron 30.0%
CAGTTACAAGTAAGATTTGT+GGG - chr1_2:54203792-54203811 None:intergenic 30.0%
ACAGTTACAAGTAAGATTTG+TGG - chr1_2:54203793-54203812 None:intergenic 30.0%
CATCTTATTAGTGATGAGTT+AGG + chr1_2:54203814-54203833 Msa0077320:intron 30.0%
! TAAGTACTTGTGAGACTTTT+GGG - chr1_2:54204253-54204272 None:intergenic 30.0%
AATCTTTGCACAAATGCATA+CGG - chr1_2:54204315-54204334 None:intergenic 30.0%
TTTAATGAATTTGATGCCAC+TGG + chr1_2:54204611-54204630 Msa0077320:CDS 30.0%
! GGAATCAATTCCTTTGAAAA+GGG - chr1_2:54204641-54204660 None:intergenic 30.0%
AAATTTATCCTCCAAATTGG+GGG + chr1_2:54204682-54204701 Msa0077320:CDS 30.0%
ACATAAGTTGCGTTAAAATC+TGG - chr1_2:54204716-54204735 None:intergenic 30.0%
AGAATCACAAGTAAAAATCG+AGG - chr1_2:54204784-54204803 None:intergenic 30.0%
CTTGTGATTCTCTTTCTAAT+TGG + chr1_2:54204793-54204812 Msa0077320:intron 30.0%
CTAATTGGAATGTTTCATAC+AGG + chr1_2:54204808-54204827 Msa0077320:intron 30.0%
GTAGAAATCATACAGAATAG+GGG - chr1_2:54205084-54205103 None:intergenic 30.0%
!!! GTGCATTGCTATTTTTCATA+GGG + chr1_2:54205260-54205279 Msa0077320:CDS 30.0%
!! ATTGTTGTGTTGCTATGTTT+TGG + chr1_2:54205390-54205409 Msa0077320:three_prime_UTR 30.0%
TAATCGGCACAATTTGATTA+TGG + chr1_2:54205428-54205447 Msa0077320:three_prime_UTR 30.0%
GAACTGAATAAAACATTGCA+AGG - chr1_2:54205546-54205565 None:intergenic 30.0%
CTAGAGAGAGAAAACAAAAA+TGG + chr1_2:54203560-54203579 Msa0077320:exon 30.0%
AGAGAGAGAAAACAAAAATG+GGG + chr1_2:54203562-54203581 Msa0077320:exon 30.0%
!! TATTTCCGTTTTTATGCATC+TGG + chr1_2:54203740-54203759 Msa0077320:intron 30.0%
!! ATTTCCGTTTTTATGCATCT+GGG + chr1_2:54203741-54203760 Msa0077320:intron 30.0%
AAAACCCAGATGCATAAAAA+CGG - chr1_2:54203748-54203767 None:intergenic 30.0%
! TTTTCACCAGATTTGGATTT+TGG + chr1_2:54203764-54203783 Msa0077320:intron 30.0%
CAGTTACAAGTAAGATTTGT+GGG - chr1_2:54203792-54203811 None:intergenic 30.0%
ACAGTTACAAGTAAGATTTG+TGG - chr1_2:54203793-54203812 None:intergenic 30.0%
CATCTTATTAGTGATGAGTT+AGG + chr1_2:54203814-54203833 Msa0077320:intron 30.0%
! TAAGTACTTGTGAGACTTTT+GGG - chr1_2:54204253-54204272 None:intergenic 30.0%
AATCTTTGCACAAATGCATA+CGG - chr1_2:54204315-54204334 None:intergenic 30.0%
TTTAATGAATTTGATGCCAC+TGG + chr1_2:54204611-54204630 Msa0077320:CDS 30.0%
! GGAATCAATTCCTTTGAAAA+GGG - chr1_2:54204641-54204660 None:intergenic 30.0%
AAATTTATCCTCCAAATTGG+GGG + chr1_2:54204682-54204701 Msa0077320:CDS 30.0%
ACATAAGTTGCGTTAAAATC+TGG - chr1_2:54204716-54204735 None:intergenic 30.0%
AGAATCACAAGTAAAAATCG+AGG - chr1_2:54204784-54204803 None:intergenic 30.0%
CTTGTGATTCTCTTTCTAAT+TGG + chr1_2:54204793-54204812 Msa0077320:intron 30.0%
CTAATTGGAATGTTTCATAC+AGG + chr1_2:54204808-54204827 Msa0077320:intron 30.0%
GTAGAAATCATACAGAATAG+GGG - chr1_2:54205084-54205103 None:intergenic 30.0%
!!! GTGCATTGCTATTTTTCATA+GGG + chr1_2:54205260-54205279 Msa0077320:CDS 30.0%
!! ATTGTTGTGTTGCTATGTTT+TGG + chr1_2:54205390-54205409 Msa0077320:three_prime_UTR 30.0%
TAATCGGCACAATTTGATTA+TGG + chr1_2:54205428-54205447 Msa0077320:three_prime_UTR 30.0%
GAACTGAATAAAACATTGCA+AGG - chr1_2:54205546-54205565 None:intergenic 30.0%
!!! AACTGTGTTTTGAAATCTGG+CGG + chr1_2:54204088-54204107 Msa0077320:intron 35.0%
!! CGGTCAAGTTTGTTTTTCAA+TGG + chr1_2:54204120-54204139 Msa0077320:intron 35.0%
! CTAAGTACTTGTGAGACTTT+TGG - chr1_2:54204254-54204273 None:intergenic 35.0%
CAAATGCATACGGATCTATT+TGG - chr1_2:54204305-54204324 None:intergenic 35.0%
ATGCATTTGTGCAAAGATTG+TGG + chr1_2:54204316-54204335 Msa0077320:intron 35.0%
TGCATTTGTGCAAAGATTGT+GGG + chr1_2:54204317-54204336 Msa0077320:intron 35.0%
!! TGTAGCTTTTTCTGCATTGA+AGG + chr1_2:54204414-54204433 Msa0077320:intron 35.0%
AGTATATGCTACATAGGCAA+AGG - chr1_2:54204500-54204519 None:intergenic 35.0%
! GGGAATCAATTCCTTTGAAA+AGG - chr1_2:54204642-54204661 None:intergenic 35.0%
GTTTCTTCTCCTATACGTTA+TGG + chr1_2:54204745-54204764 Msa0077320:intron 35.0%
TCAGACAATCCATAACGTAT+AGG - chr1_2:54204757-54204776 None:intergenic 35.0%
TGCCACTATCATATTTCTCA+AGG + chr1_2:54204844-54204863 Msa0077320:CDS 35.0%
GCCACTATCATATTTCTCAA+GGG + chr1_2:54204845-54204864 Msa0077320:CDS 35.0%
TCCCTTGAGAAATATGATAG+TGG - chr1_2:54204849-54204868 None:intergenic 35.0%
ATCATATTTCTCAAGGGAGT+TGG + chr1_2:54204851-54204870 Msa0077320:CDS 35.0%
GGAGGAATTCTATTTGTGTT+TGG + chr1_2:54204872-54204891 Msa0077320:CDS 35.0%
ATTCATTCTTCAGCTAACAG+AGG - chr1_2:54204933-54204952 None:intergenic 35.0%
! TGAAAGCTACATAAGTTGAG+AGG - chr1_2:54205045-54205064 None:intergenic 35.0%
ACTTATGTAGCTTTCACATC+TGG + chr1_2:54205049-54205068 Msa0077320:intron 35.0%
TTTAGCAACAAACCGTATTC+CGG - chr1_2:54205125-54205144 None:intergenic 35.0%
GTTGCTAAATGAGTTCATTC+AGG + chr1_2:54205136-54205155 Msa0077320:CDS 35.0%
!!! GGTGCATTGCTATTTTTCAT+AGG + chr1_2:54205259-54205278 Msa0077320:CDS 35.0%
! TTCTTCCTCAACTGTTTTCT+GGG - chr1_2:54205301-54205320 None:intergenic 35.0%
!! TCTAGACTGCTTTTGCTTTT+GGG - chr1_2:54205327-54205346 None:intergenic 35.0%
!! TTCTAGACTGCTTTTGCTTT+TGG - chr1_2:54205328-54205347 None:intergenic 35.0%
TGTGTCTGTTTACTAAGAAC+AGG + chr1_2:54205360-54205379 Msa0077320:three_prime_UTR 35.0%
!!! TGTGTTGCTATGTTTTGGAA+TGG + chr1_2:54205395-54205414 Msa0077320:three_prime_UTR 35.0%
GGCACAATTTGATTATGGAT+TGG + chr1_2:54205433-54205452 Msa0077320:three_prime_UTR 35.0%
TTACGATTCTGTTGTACTTG+CGG + chr1_2:54205474-54205493 Msa0077320:three_prime_UTR 35.0%
!!! AACTGTGTTTTGAAATCTGG+CGG + chr1_2:54204088-54204107 Msa0077320:intron 35.0%
!! CGGTCAAGTTTGTTTTTCAA+TGG + chr1_2:54204120-54204139 Msa0077320:intron 35.0%
! CTAAGTACTTGTGAGACTTT+TGG - chr1_2:54204254-54204273 None:intergenic 35.0%
CAAATGCATACGGATCTATT+TGG - chr1_2:54204305-54204324 None:intergenic 35.0%
ATGCATTTGTGCAAAGATTG+TGG + chr1_2:54204316-54204335 Msa0077320:intron 35.0%
TGCATTTGTGCAAAGATTGT+GGG + chr1_2:54204317-54204336 Msa0077320:intron 35.0%
!! TGTAGCTTTTTCTGCATTGA+AGG + chr1_2:54204414-54204433 Msa0077320:intron 35.0%
AGTATATGCTACATAGGCAA+AGG - chr1_2:54204500-54204519 None:intergenic 35.0%
! GGGAATCAATTCCTTTGAAA+AGG - chr1_2:54204642-54204661 None:intergenic 35.0%
GTTTCTTCTCCTATACGTTA+TGG + chr1_2:54204745-54204764 Msa0077320:intron 35.0%
TCAGACAATCCATAACGTAT+AGG - chr1_2:54204757-54204776 None:intergenic 35.0%
TGCCACTATCATATTTCTCA+AGG + chr1_2:54204844-54204863 Msa0077320:CDS 35.0%
GCCACTATCATATTTCTCAA+GGG + chr1_2:54204845-54204864 Msa0077320:CDS 35.0%
TCCCTTGAGAAATATGATAG+TGG - chr1_2:54204849-54204868 None:intergenic 35.0%
ATCATATTTCTCAAGGGAGT+TGG + chr1_2:54204851-54204870 Msa0077320:CDS 35.0%
GGAGGAATTCTATTTGTGTT+TGG + chr1_2:54204872-54204891 Msa0077320:CDS 35.0%
ATTCATTCTTCAGCTAACAG+AGG - chr1_2:54204933-54204952 None:intergenic 35.0%
! TGAAAGCTACATAAGTTGAG+AGG - chr1_2:54205045-54205064 None:intergenic 35.0%
ACTTATGTAGCTTTCACATC+TGG + chr1_2:54205049-54205068 Msa0077320:intron 35.0%
TTTAGCAACAAACCGTATTC+CGG - chr1_2:54205125-54205144 None:intergenic 35.0%
GTTGCTAAATGAGTTCATTC+AGG + chr1_2:54205136-54205155 Msa0077320:CDS 35.0%
!!! GGTGCATTGCTATTTTTCAT+AGG + chr1_2:54205259-54205278 Msa0077320:CDS 35.0%
! TTCTTCCTCAACTGTTTTCT+GGG - chr1_2:54205301-54205320 None:intergenic 35.0%
!! TCTAGACTGCTTTTGCTTTT+GGG - chr1_2:54205327-54205346 None:intergenic 35.0%
!! TTCTAGACTGCTTTTGCTTT+TGG - chr1_2:54205328-54205347 None:intergenic 35.0%
TGTGTCTGTTTACTAAGAAC+AGG + chr1_2:54205360-54205379 Msa0077320:three_prime_UTR 35.0%
!!! TGTGTTGCTATGTTTTGGAA+TGG + chr1_2:54205395-54205414 Msa0077320:three_prime_UTR 35.0%
GGCACAATTTGATTATGGAT+TGG + chr1_2:54205433-54205452 Msa0077320:three_prime_UTR 35.0%
TTACGATTCTGTTGTACTTG+CGG + chr1_2:54205474-54205493 Msa0077320:three_prime_UTR 35.0%
CTCTCCTTAATCTGACCAAT+GGG - chr1_2:54203445-54203464 None:intergenic 40.0%
ACTCTCCTTAATCTGACCAA+TGG - chr1_2:54203446-54203465 None:intergenic 40.0%
!!! TGTTTTCTCTCTCTAGCTCT+CGG - chr1_2:54203556-54203575 None:intergenic 40.0%
! ATCTGGGTTTTCACCAGATT+TGG + chr1_2:54203757-54203776 Msa0077320:intron 40.0%
TGGGATCCAAAATCCAAATC+TGG - chr1_2:54203773-54203792 None:intergenic 40.0%
AAATCTGGCGGAATAGAAGA+CGG + chr1_2:54204100-54204119 Msa0077320:intron 40.0%
!! TGTCTTGGAGACTTGTTACT+TGG + chr1_2:54204371-54204390 Msa0077320:intron 40.0%
AATGAATTTGATGCCACTGG+TGG + chr1_2:54204614-54204633 Msa0077320:CDS 40.0%
TTCCTCAAAATCGTGAGCTT+GGG - chr1_2:54204662-54204681 None:intergenic 40.0%
TTTCCTCAAAATCGTGAGCT+TGG - chr1_2:54204663-54204682 None:intergenic 40.0%
ATATTTCTCAAGGGAGTTGG+AGG + chr1_2:54204854-54204873 Msa0077320:CDS 40.0%
!! GGAAGCATTTACCAAGAGAA+AGG - chr1_2:54204912-54204931 None:intergenic 40.0%
CAACTATAGACCTACTAAGC+CGG + chr1_2:54205103-54205122 Msa0077320:CDS 40.0%
!! CTTGCTATTTCCTGTGTTTC+TGG + chr1_2:54205169-54205188 Msa0077320:intron 40.0%
CAATTCCCAGAAAACAGTTG+AGG + chr1_2:54205293-54205312 Msa0077320:CDS 40.0%
! CTTCTTCCTCAACTGTTTTC+TGG - chr1_2:54205302-54205321 None:intergenic 40.0%
!! CTAGACTGCTTTTGCTTTTG+GGG - chr1_2:54205326-54205345 None:intergenic 40.0%
CTCTCCTTAATCTGACCAAT+GGG - chr1_2:54203445-54203464 None:intergenic 40.0%
ACTCTCCTTAATCTGACCAA+TGG - chr1_2:54203446-54203465 None:intergenic 40.0%
!!! TGTTTTCTCTCTCTAGCTCT+CGG - chr1_2:54203556-54203575 None:intergenic 40.0%
! ATCTGGGTTTTCACCAGATT+TGG + chr1_2:54203757-54203776 Msa0077320:intron 40.0%
TGGGATCCAAAATCCAAATC+TGG - chr1_2:54203773-54203792 None:intergenic 40.0%
AAATCTGGCGGAATAGAAGA+CGG + chr1_2:54204100-54204119 Msa0077320:intron 40.0%
!! TGTCTTGGAGACTTGTTACT+TGG + chr1_2:54204371-54204390 Msa0077320:intron 40.0%
AATGAATTTGATGCCACTGG+TGG + chr1_2:54204614-54204633 Msa0077320:CDS 40.0%
TTCCTCAAAATCGTGAGCTT+GGG - chr1_2:54204662-54204681 None:intergenic 40.0%
TTTCCTCAAAATCGTGAGCT+TGG - chr1_2:54204663-54204682 None:intergenic 40.0%
ATATTTCTCAAGGGAGTTGG+AGG + chr1_2:54204854-54204873 Msa0077320:CDS 40.0%
!! GGAAGCATTTACCAAGAGAA+AGG - chr1_2:54204912-54204931 None:intergenic 40.0%
CAACTATAGACCTACTAAGC+CGG + chr1_2:54205103-54205122 Msa0077320:CDS 40.0%
!! CTTGCTATTTCCTGTGTTTC+TGG + chr1_2:54205169-54205188 Msa0077320:intron 40.0%
CAATTCCCAGAAAACAGTTG+AGG + chr1_2:54205293-54205312 Msa0077320:CDS 40.0%
! CTTCTTCCTCAACTGTTTTC+TGG - chr1_2:54205302-54205321 None:intergenic 40.0%
!! CTAGACTGCTTTTGCTTTTG+GGG - chr1_2:54205326-54205345 None:intergenic 40.0%
CCTTAATCTGACCAATGGGA+AGG - chr1_2:54203441-54203460 None:intergenic 45.0%
CCTTCCCATTGGTCAGATTA+AGG + chr1_2:54203438-54203457 Msa0077320:five_prime_UTR 45.0%
AATGGGGTTCTTCTCCTTCA+TGG + chr1_2:54203578-54203597 Msa0077320:exon 45.0%
ATGGGGTTCTTCTCCTTCAT+GGG + chr1_2:54203579-54203598 Msa0077320:CDS 45.0%
AGAGGGAAGCAAAGAGAACA+CGG - chr1_2:54203605-54203624 None:intergenic 45.0%
! TTCCCAAGCTCACGATTTTG+AGG + chr1_2:54204657-54204676 Msa0077320:CDS 45.0%
TTTGTGTTTGGCAGCACGTT+TGG + chr1_2:54204884-54204903 Msa0077320:CDS 45.0%
CACGTTTGGATCCTTTCTCT+TGG + chr1_2:54204898-54204917 Msa0077320:CDS 45.0%
AGACCTACTAAGCCGGAATA+CGG + chr1_2:54205110-54205129 Msa0077320:CDS 45.0%
AAACCGTATTCCGGCTTAGT+AGG - chr1_2:54205116-54205135 None:intergenic 45.0%
!! TTTCCTGTGTTTCTGGCTGA+TGG + chr1_2:54205176-54205195 Msa0077320:intron 45.0%
AAACCATCAGCCAGAAACAC+AGG - chr1_2:54205182-54205201 None:intergenic 45.0%
! TTGCAGAACGCAGCACTTTT+TGG + chr1_2:54205238-54205257 Msa0077320:intron 45.0%
CCTTAATCTGACCAATGGGA+AGG - chr1_2:54203441-54203460 None:intergenic 45.0%
CCTTCCCATTGGTCAGATTA+AGG + chr1_2:54203438-54203457 Msa0077320:five_prime_UTR 45.0%
AATGGGGTTCTTCTCCTTCA+TGG + chr1_2:54203578-54203597 Msa0077320:exon 45.0%
ATGGGGTTCTTCTCCTTCAT+GGG + chr1_2:54203579-54203598 Msa0077320:CDS 45.0%
AGAGGGAAGCAAAGAGAACA+CGG - chr1_2:54203605-54203624 None:intergenic 45.0%
! TTCCCAAGCTCACGATTTTG+AGG + chr1_2:54204657-54204676 Msa0077320:CDS 45.0%
TTTGTGTTTGGCAGCACGTT+TGG + chr1_2:54204884-54204903 Msa0077320:CDS 45.0%
CACGTTTGGATCCTTTCTCT+TGG + chr1_2:54204898-54204917 Msa0077320:CDS 45.0%
AGACCTACTAAGCCGGAATA+CGG + chr1_2:54205110-54205129 Msa0077320:CDS 45.0%
AAACCGTATTCCGGCTTAGT+AGG - chr1_2:54205116-54205135 None:intergenic 45.0%
!! TTTCCTGTGTTTCTGGCTGA+TGG + chr1_2:54205176-54205195 Msa0077320:intron 45.0%
AAACCATCAGCCAGAAACAC+AGG - chr1_2:54205182-54205201 None:intergenic 45.0%
! TTGCAGAACGCAGCACTTTT+TGG + chr1_2:54205238-54205257 Msa0077320:intron 45.0%
AAAGAGAACACGGCCCATGA+AGG - chr1_2:54203595-54203614 None:intergenic 50.0%
GAGAACTGACATCTGCCATG+CGG - chr1_2:54203637-54203656 None:intergenic 50.0%
!! GAGACTTTTGGGAGAGCTCA+TGG - chr1_2:54204242-54204261 None:intergenic 50.0%
! CTTTGAAAAGGGTCCACCAG+TGG - chr1_2:54204630-54204649 None:intergenic 50.0%
!! CACTGGTGGACCCTTTTCAA+AGG + chr1_2:54204628-54204647 Msa0077320:CDS 50.0%
TATCGCTACCCCCAATTTGG+AGG - chr1_2:54204693-54204712 None:intergenic 50.0%
TGGTATCGCTACCCCCAATT+TGG - chr1_2:54204696-54204715 None:intergenic 50.0%
AAAGAGAACACGGCCCATGA+AGG - chr1_2:54203595-54203614 None:intergenic 50.0%
GAGAACTGACATCTGCCATG+CGG - chr1_2:54203637-54203656 None:intergenic 50.0%
!! GAGACTTTTGGGAGAGCTCA+TGG - chr1_2:54204242-54204261 None:intergenic 50.0%
! CTTTGAAAAGGGTCCACCAG+TGG - chr1_2:54204630-54204649 None:intergenic 50.0%
!! CACTGGTGGACCCTTTTCAA+AGG + chr1_2:54204628-54204647 Msa0077320:CDS 50.0%
TATCGCTACCCCCAATTTGG+AGG - chr1_2:54204693-54204712 None:intergenic 50.0%
TGGTATCGCTACCCCCAATT+TGG - chr1_2:54204696-54204715 None:intergenic 50.0%
CCATGCGGAGAGGATGAAGA+GGG - chr1_2:54203622-54203641 None:intergenic 55.0%
CCATGCGGAGAGGATGAAGA+GGG - chr1_2:54203622-54203641 None:intergenic 55.0%
CCCTCTTCATCCTCTCCGCA+TGG + chr1_2:54203619-54203638 Msa0077320:CDS 60.0%
GCCATGCGGAGAGGATGAAG+AGG - chr1_2:54203623-54203642 None:intergenic 60.0%
CTGACATCTGCCATGCGGAG+AGG - chr1_2:54203632-54203651 None:intergenic 60.0%
CCCTCTTCATCCTCTCCGCA+TGG + chr1_2:54203619-54203638 Msa0077320:CDS 60.0%
GCCATGCGGAGAGGATGAAG+AGG - chr1_2:54203623-54203642 None:intergenic 60.0%
CTGACATCTGCCATGCGGAG+AGG - chr1_2:54203632-54203651 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 54203431 54205620 54203431 ID=Msa0077320;Name=Msa0077320
chr1_2 mRNA 54203431 54205620 54203431 ID=Msa0077320-mRNA-1;Parent=Msa0077320;Name=Msa0077320-mRNA-1;_AED=0.11;_eAED=0.11;_QI=148|0.75|0.8|1|1|1|5|275|166
chr1_2 exon 54203431 54203646 54203431 ID=Msa0077320-mRNA-1:exon:16730;Parent=Msa0077320-mRNA-1
chr1_2 exon 54204592 54204733 54204592 ID=Msa0077320-mRNA-1:exon:16731;Parent=Msa0077320-mRNA-1
chr1_2 exon 54204830 54204919 54204830 ID=Msa0077320-mRNA-1:exon:16732;Parent=Msa0077320-mRNA-1
chr1_2 exon 54205059 54205157 54205059 ID=Msa0077320-mRNA-1:exon:16733;Parent=Msa0077320-mRNA-1
chr1_2 exon 54205244 54205620 54205244 ID=Msa0077320-mRNA-1:exon:16734;Parent=Msa0077320-mRNA-1
chr1_2 five_prime_UTR 54203431 54203578 54203431 ID=Msa0077320-mRNA-1:five_prime_utr;Parent=Msa0077320-mRNA-1
chr1_2 CDS 54203579 54203646 54203579 ID=Msa0077320-mRNA-1:cds;Parent=Msa0077320-mRNA-1
chr1_2 CDS 54204592 54204733 54204592 ID=Msa0077320-mRNA-1:cds;Parent=Msa0077320-mRNA-1
chr1_2 CDS 54204830 54204919 54204830 ID=Msa0077320-mRNA-1:cds;Parent=Msa0077320-mRNA-1
chr1_2 CDS 54205059 54205157 54205059 ID=Msa0077320-mRNA-1:cds;Parent=Msa0077320-mRNA-1
chr1_2 CDS 54205244 54205345 54205244 ID=Msa0077320-mRNA-1:cds;Parent=Msa0077320-mRNA-1
chr1_2 three_prime_UTR 54205346 54205620 54205346 ID=Msa0077320-mRNA-1:three_prime_utr;Parent=Msa0077320-mRNA-1
Gene Sequence

>Msa0077320

ATGGGGTTCTTCTCCTTCATGGGCCGTGTTCTCTTTGCTTCCCTCTTCATCCTCTCCGCATGGCAGATAATTTATGTTGTTGCTAGGTTTAATGAATTTGATGCCACTGGTGGACCCTTTTCAAAGGAATTGATTCCCAAGCTCACGATTTTGAGGAAAAATTTATCCTCCAAATTGGGGGTAGCGATACCAGATTTTAACGCAACTTATGTTCGTCAATTCGTTGCCACTATCATATTTCTCAAGGGAGTTGGAGGAATTCTATTTGTGTTTGGCAGCACGTTTGGATCCTTTCTCTTGCTTTCACATCTGGCGCTTACTACCCCTATTCTGTATGATTTCTACAACTATAGACCTACTAAGCCGGAATACGGTTTGTTGCTAAATGAGTTCATTCAGAACGCAGCACTTTTTGGTGCATTGCTATTTTTCATAGGGATGAAGCACTCAATTCCCAGAAAACAGTTGAGGAAGAAGACCCCAAAAGCAAAAGCAGTCTAG
Protein sequence

>Msa0077320

MGFFSFMGRVLFASLFILSAWQIIYVVARFNEFDATGGPFSKELIPKLTILRKNLSSKLGVAIPDFNATYVRQFVATIIFLKGVGGILFVFGSTFGSFLLLSHLALTTPILYDFYNYRPTKPEYGLLLNEFIQNAALFGALLFFIGMKHSIPRKQLRKKTPKAKAV*