AlfalfaGEDB | Msa1255220

M. sativa cultivar zhongmu-4 / Msa1255220

KEGG: K21630
GO: GO:0000003 GO:0000976 GO:0000977 GO:0000981 GO:0001012 GO:0001067 GO:0001085 GO:0001228 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005667 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009909 GO:0009987 GO:0010154 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0040034 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0046983 GO:0048316 GO:0048367 GO:0048437 GO:0048506 GO:0048509 GO:0048510 GO:0048518 GO:0048522 GO:0048580 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051173 GO:0051239 GO:0051252 GO:0051254 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0090567 GO:0097159 GO:0097659 GO:0099402 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000026 GO:2000112 GO:2000241 GO:2001141
PFAM: PF00320.30

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1255220	XP_003629440.1	90.041	241	15	3	1	235	1	238	3.02e-124	362

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1255220	sp\|Q8LC79\|GAT18_ARATH	38.112	286	100	11	20	235	17	295	9.30e-43	149

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1255220	G7LE01	90.041	241	15	3	1	235	1	238	1.44e-124	362

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TFs/TRs:

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Gene ID	Type	Classification
Msa1255220	TF	C2C2-GATA

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0179670	Msa1255220	0.801466	8.887017e-49	-8.615850e-47
Msa0205440	Msa1255220	0.802089	6.618880e-49	-8.615850e-47
Msa0420680	Msa1255220	0.829944	3.877020e-55	-8.615850e-47
Msa0512520	Msa1255220	0.826307	2.914316e-54	-8.615850e-47
Msa1014320	Msa1255220	0.804411	2.188273e-49	-8.615850e-47
Msa1055310	Msa1255220	0.813485	2.499177e-51	-8.615850e-47
Msa1131150	Msa1255220	0.824506	7.779822e-54	-8.615850e-47
Msa1187760	Msa1255220	0.811931	5.470121e-51	-8.615850e-47
Msa1255220	Msa1342810	0.803548	3.307773e-49	-8.615850e-47
Msa1255220	Msa1356220	0.835985	1.219738e-56	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1255220	MtrunA17_Chr8g0373231	90.041	241	15	3	1	235	1	238	2.78e-128	362
Msa1255220	MtrunA17_Chr5g0404131	54.878	246	79	12	1	225	71	305	2.62e-67	209
Msa1255220	MtrunA17_Chr1g0151461	59.322	59	21	2	114	172	179	234	1.50e-13	68.6
Msa1255220	MtrunA17_Chr7g0271861	65.789	38	13	0	120	157	18	55	1.74e-13	65.5
Msa1255220	MtrunA17_Chr4g0071301	55.385	65	27	2	89	153	159	221	2.07e-13	68.6
Msa1255220	MtrunA17_Chr4g0068031	67.568	37	12	0	124	160	30	66	1.08e-12	63.5
Msa1255220	MtrunA17_Chr2g0326771	29.412	153	90	4	20	156	98	248	2.37e-12	65.1
Msa1255220	MtrunA17_Chr1g0213161	48.148	54	28	0	103	156	131	184	2.95e-12	64.3
Msa1255220	MtrunA17_Chr4g0028961	56.098	41	18	0	116	156	224	264	6.27e-12	63.9
Msa1255220	MtrunA17_Chr1g0199671	56.250	48	15	1	109	156	233	274	1.53e-11	63.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1255220	AT3G50870.1	38.112	286	100	11	20	235	17	295	9.45e-44	149
Msa1255220	AT2G18380.1	49.045	157	68	3	23	178	1	146	3.11e-38	132
Msa1255220	AT4G36620.1	41.063	207	84	6	53	234	17	210	3.52e-32	117
Msa1255220	AT5G56860.1	64.000	50	15	2	110	157	215	263	2.80e-13	68.9
Msa1255220	AT4G26150.1	73.684	38	9	1	117	154	193	229	8.73e-13	67.0
Msa1255220	AT5G26930.1	68.571	35	11	0	126	160	28	62	9.05e-13	63.5
Msa1255220	AT5G49300.1	71.429	35	10	0	126	160	38	72	1.65e-12	63.2
Msa1255220	AT3G06740.1	71.429	35	10	0	126	160	43	77	2.82e-12	62.8
Msa1255220	AT3G54810.1	48.571	70	31	2	109	174	215	283	6.75e-12	64.3
Msa1255220	AT3G54810.2	48.571	70	31	2	109	174	215	283	6.75e-12	64.3

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Find 56 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAGCAGCATAATGGAGTC+AGG	0.496113	8_2:-53645031	Msa1255220:CDS
AACGTGTGGAAGGGGTAGCA+AGG	0.566554	8_2:+53645642	None:intergenic
AAGCAAGGAGTAGTTAGAAT+TGG	0.345149	8_2:+53645751	None:intergenic
AATCATAATGATCATGTTGT+TGG	0.413223	8_2:+53645717	None:intergenic
AATTAGCAAGAGAAGAGCGA+CGG	0.619540	8_2:+53645594	None:intergenic
ACACCACTTCTACACCCCTT+TGG	0.442678	8_2:-53645445	Msa1255220:CDS
ACCGGAGGGAGTATTAACGT+AGG	0.802024	8_2:+53644789	None:intergenic
ACGAGGACCATTCCTCCAAA+GGG	0.492453	8_2:+53645430	None:intergenic
ACGTGTGGAAGGGGTAGCAA+GGG	0.615165	8_2:+53645643	None:intergenic
AGAGAGAAGAGCGAACGCTG+CGG	0.651764	8_2:-53645074	Msa1255220:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCAAGCAAAGGAAACAATAT+TGG	-	chr8_2:53645155-53645174	Msa1255220:intron	30.0%
GGTAATGTATGTTTACCTTT+GGG	+	chr8_2:53645271-53645290	None:intergenic	30.0%
AACATTTATAGTCTACTACG+AGG	+	chr8_2:53645330-53645349	None:intergenic	30.0%
CGTAGTAGACTATAAATGTT+CGG	-	chr8_2:53645330-53645349	Msa1255220:intron	30.0%
ACAACAACAACAACAATTCA+TGG	-	chr8_2:53645673-53645692	Msa1255220:CDS	30.0%
GATTACACAAGATGAAACAT+TGG	-	chr8_2:53645819-53645838	Msa1255220:CDS	30.0%
TCAAGCAAAGGAAACAATAT+TGG	-	chr8_2:53645155-53645174	Msa1255220:intron	30.0%
GGTAATGTATGTTTACCTTT+GGG	+	chr8_2:53645271-53645290	None:intergenic	30.0%
AACATTTATAGTCTACTACG+AGG	+	chr8_2:53645330-53645349	None:intergenic	30.0%
CGTAGTAGACTATAAATGTT+CGG	-	chr8_2:53645330-53645349	Msa1255220:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	53644768	53645927	53644768	ID=Msa1255220;Name=Msa1255220
chr8_2	mRNA	53644768	53645927	53644768	ID=Msa1255220-mRNA-1;Parent=Msa1255220;Name=Msa1255220-mRNA-1;_AED=0.00;_eAED=0.00;_QI=72\|1\|1\|1\|1\|1\|2\|94\|235
chr8_2	exon	53644768	53645134	53644768	ID=Msa1255220-mRNA-1:exon:16622;Parent=Msa1255220-mRNA-1
chr8_2	exon	53645421	53645927	53645421	ID=Msa1255220-mRNA-1:exon:16621;Parent=Msa1255220-mRNA-1
chr8_2	five_prime_UTR	53645856	53645927	53645856	ID=Msa1255220-mRNA-1:five_prime_utr;Parent=Msa1255220-mRNA-1
chr8_2	CDS	53645421	53645855	53645421	ID=Msa1255220-mRNA-1:cds;Parent=Msa1255220-mRNA-1
chr8_2	CDS	53644862	53645134	53644862	ID=Msa1255220-mRNA-1:cds;Parent=Msa1255220-mRNA-1
chr8_2	three_prime_UTR	53644768	53644861	53644768	ID=Msa1255220-mRNA-1:three_prime_utr;Parent=Msa1255220-mRNA-1

Gene Sequence

>Msa1255220

ATGATGCATCATTGTTGTAGTAGCTCTCAACAAGGTCACATAATGGGAGGAACATGCACATGTGGTATGTTTCATCCTCAAACCAATTCTAACTACTCCTTGCTTTACTCTAACTACCAACAACATGATCATTATGATTTTGACCCTTACTCTTGCACTACTCCATCTTCTTCTGTTGATTGCACACTCTCCCTTGCTACCCCTTCCACACGTTTATCTGAAGACCAAGATAAACGAAACCGTCGCTCTTCTCTTGCTAATTTTTTTTGTAGCAAATCTTCCAACACTAAACATAATTCTCAATCTCAATCCAAGTCAAGCAAAGGAAACAATATTGGTAGTAACTCTAACAATGATTCTCTTCTTTCTCGTCGATGTGCTAGCTGTGACACCACTTCTACACCCCTTTGGAGGAATGGTCCTCGTGGCCCAAAGTCACTATGCAATGCTTGCGGGATTAGATACAAGAAGGAAGAGAGAAGAGCGAACGCTGCGGTTGCCACTACAGCCGCGACTAACAGCAGCATAATGGAGTCAGGAAACTTCTACAACAACAACAACAATTCATGGTACTCACAACCACAGAGTCAAATGTATGGCAGTGAGCTAAATTTCATGGATGATTCAGATGCAGAATCAGAGAATGGCATTCCAAATTTCCTTTCTTGGAGACTCAACACAAGTCTTGTTCACGATTACACAAGATGA

Protein sequence

>Msa1255220

MMHHCCSSSQQGHIMGGTCTCGMFHPQTNSNYSLLYSNYQQHDHYDFDPYSCTTPSSSVDCTLSLATPSTRLSEDQDKRNRRSSLANFFCSKSSNTKHNSQSQSKSSKGNNIGSNSNNDSLLSRRCASCDTTSTPLWRNGPRGPKSLCNACGIRYKKEERRANAAVATTAATNSSIMESGNFYNNNNNSWYSQPQSQMYGSELNFMDDSDAESENGIPNFLSWRLNTSLVHDYTR*