AlfalfaGEDB | Msa1255220

M. sativa cultivar zhongmu-4 / Msa1255220

KEGG: K21630
GO: GO:0000003 GO:0000976 GO:0000977 GO:0000981 GO:0001012 GO:0001067 GO:0001085 GO:0001228 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005667 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009909 GO:0009987 GO:0010154 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0040034 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0046983 GO:0048316 GO:0048367 GO:0048437 GO:0048506 GO:0048509 GO:0048510 GO:0048518 GO:0048522 GO:0048580 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051173 GO:0051239 GO:0051252 GO:0051254 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0090567 GO:0097159 GO:0097659 GO:0099402 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000026 GO:2000112 GO:2000241 GO:2001141
PFAM: PF00320.30

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1255220	XP_003629440.1	90.041	241	15	3	1	235	1	238	3.02e-124	362

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1255220	sp\|Q8LC79\|GAT18_ARATH	38.112	286	100	11	20	235	17	295	9.30e-43	149

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1255220	G7LE01	90.041	241	15	3	1	235	1	238	1.44e-124	362

TFs/TRs:

Gene ID	Type	Classification
Msa1255220	TF	C2C2-GATA

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0179670	Msa1255220	0.801466	8.887017e-49	-8.615850e-47
Msa0205440	Msa1255220	0.802089	6.618880e-49	-8.615850e-47
Msa1255220	Msa1342810	0.803548	3.307773e-49	-8.615850e-47
Msa1255220	Msa1356220	0.835985	1.219738e-56	-8.615850e-47
Msa0420680	Msa1255220	0.829944	3.877020e-55	-8.615850e-47
Msa1014320	Msa1255220	0.804411	2.188273e-49	-8.615850e-47
Msa1055310	Msa1255220	0.813485	2.499177e-51	-8.615850e-47
Msa1131150	Msa1255220	0.824506	7.779822e-54	-8.615850e-47
Msa1187760	Msa1255220	0.811931	5.470121e-51	-8.615850e-47
Msa0512520	Msa1255220	0.826307	2.914316e-54	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1255220	MtrunA17_Chr8g0373231	90.041	241	15	3	1	235	1	238	2.78e-128	362
Msa1255220	MtrunA17_Chr5g0404131	54.878	246	79	12	1	225	71	305	2.62e-67	209
Msa1255220	MtrunA17_Chr1g0151461	59.322	59	21	2	114	172	179	234	1.50e-13	68.6
Msa1255220	MtrunA17_Chr7g0271861	65.789	38	13	0	120	157	18	55	1.74e-13	65.5
Msa1255220	MtrunA17_Chr4g0071301	55.385	65	27	2	89	153	159	221	2.07e-13	68.6
Msa1255220	MtrunA17_Chr4g0068031	67.568	37	12	0	124	160	30	66	1.08e-12	63.5
Msa1255220	MtrunA17_Chr2g0326771	29.412	153	90	4	20	156	98	248	2.37e-12	65.1
Msa1255220	MtrunA17_Chr1g0213161	48.148	54	28	0	103	156	131	184	2.95e-12	64.3
Msa1255220	MtrunA17_Chr4g0028961	56.098	41	18	0	116	156	224	264	6.27e-12	63.9
Msa1255220	MtrunA17_Chr1g0199671	56.250	48	15	1	109	156	233	274	1.53e-11	63.2
Msa1255220	MtrunA17_Chr3g0145121	51.111	45	22	0	112	156	203	247	1.93e-11	62.4
Msa1255220	MtrunA17_Chr8g0372291	45.455	55	30	0	102	156	196	250	8.36e-11	60.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1255220	AT3G50870.1	38.112	286	100	11	20	235	17	295	9.45e-44	149
Msa1255220	AT2G18380.1	49.045	157	68	3	23	178	1	146	3.11e-38	132
Msa1255220	AT4G36620.1	41.063	207	84	6	53	234	17	210	3.52e-32	117
Msa1255220	AT5G56860.1	64.000	50	15	2	110	157	215	263	2.80e-13	68.9
Msa1255220	AT4G26150.1	73.684	38	9	1	117	154	193	229	8.73e-13	67.0
Msa1255220	AT5G26930.1	68.571	35	11	0	126	160	28	62	9.05e-13	63.5
Msa1255220	AT5G49300.1	71.429	35	10	0	126	160	38	72	1.65e-12	63.2
Msa1255220	AT3G06740.1	71.429	35	10	0	126	160	43	77	2.82e-12	62.8
Msa1255220	AT3G54810.1	48.571	70	31	2	109	174	215	283	6.75e-12	64.3
Msa1255220	AT3G54810.2	48.571	70	31	2	109	174	215	283	6.75e-12	64.3
Msa1255220	AT4G32890.1	30.769	143	74	5	22	156	104	229	2.82e-11	62.4
Msa1255220	AT1G08010.2	58.537	41	16	1	116	156	213	252	2.83e-11	62.4
Msa1255220	AT1G08010.1	58.537	41	16	1	116	156	213	252	2.83e-11	62.4
Msa1255220	AT1G08010.3	58.537	41	16	1	116	156	213	252	2.83e-11	62.4
Msa1255220	AT4G34680.1	34.862	109	60	4	52	156	111	212	4.37e-11	61.6
Msa1255220	AT4G34680.2	34.862	109	60	4	52	156	111	212	4.37e-11	61.6
Msa1255220	AT3G20750.1	66.667	33	11	0	129	161	164	196	9.49e-11	60.1

Find 56 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCATTCCAAATTTCCTTTCT+TGG	0.186888	8_2:-53644901	Msa1255220:CDS
AGTCTCCAAGAAAGGAAATT+TGG	0.258564	8_2:+53644896	None:intergenic
AAGCAAGGAGTAGTTAGAAT+TGG	0.345149	8_2:+53645751	None:intergenic
ATTGTTTCCTTTGCTTGACT+TGG	0.370949	8_2:+53645523	None:intergenic
TATTATGTTGCAATGTTGTA+TGG	0.378046	8_2:+53645904	None:intergenic
TCAAGCAAAGGAAACAATAT+TGG	0.387578	8_2:-53645518	Msa1255220:CDS
AATCATAATGATCATGTTGT+TGG	0.413223	8_2:+53645717	None:intergenic
GATGGAGTAGTGCAAGAGTA+AGG	0.419041	8_2:+53645689	None:intergenic
CTACAACAATGATGCATCAT+TGG	0.421362	8_2:+53645836	None:intergenic
AGTCACTATGCAATGCTTGC+GGG	0.430912	8_2:-53645114	Msa1255220:CDS
CGCGACTAACAGCAGCATAA+TGG	0.440251	8_2:-53645038	Msa1255220:CDS
ACACCACTTCTACACCCCTT+TGG	0.442678	8_2:-53645445	Msa1255220:CDS
AGCGACGGTTTCGTTTATCT+TGG	0.447089	8_2:+53645609	None:intergenic
TCTCAACAAGGTCACATAAT+GGG	0.447815	8_2:-53645809	Msa1255220:CDS
CTCTCAACAAGGTCACATAA+TGG	0.454874	8_2:-53645810	Msa1255220:CDS
GAGTAGTTAGAATTGGTTTG+AGG	0.463259	8_2:+53645758	None:intergenic
CCACTTCTACACCCCTTTGG+AGG	0.484365	8_2:-53645442	Msa1255220:CDS
TTGAGAATTATGTTTAGTGT+TGG	0.488709	8_2:+53645553	None:intergenic
ACGAGGACCATTCCTCCAAA+GGG	0.492453	8_2:+53645430	None:intergenic
AACAGCAGCATAATGGAGTC+AGG	0.496113	8_2:-53645031	Msa1255220:CDS
TGGCAGTGAGCTAAATTTCA+TGG	0.499631	8_2:-53644951	Msa1255220:CDS
TCTACACCCCTTTGGAGGAA+TGG	0.501186	8_2:-53645437	Msa1255220:CDS
TTCAGATAAACGTGTGGAAG+GGG	0.521943	8_2:+53645634	None:intergenic
TTGTGTTGAGTCTCCAAGAA+AGG	0.529190	8_2:+53644888	None:intergenic
CAGTCACTATGCAATGCTTG+CGG	0.541543	8_2:-53645115	Msa1255220:CDS
TTGGTAGTTAGAGTAAAGCA+AGG	0.544326	8_2:+53645736	None:intergenic
CACGAGGACCATTCCTCCAA+AGG	0.545071	8_2:+53645429	None:intergenic
CTTCAGATAAACGTGTGGAA+GGG	0.558291	8_2:+53645633	None:intergenic
GTGCAATCAACAGAAGAAGA+TGG	0.561538	8_2:+53645671	None:intergenic
CAACCACAGAGTCAAATGTA+TGG	0.561677	8_2:-53644971	Msa1255220:CDS
CCTCCAAAGGGGTGTAGAAG+TGG	0.563224	8_2:+53645442	None:intergenic
GCTGTTAGTCGCGGCTGTAG+TGG	0.564500	8_2:+53645048	None:intergenic
AACGTGTGGAAGGGGTAGCA+AGG	0.566554	8_2:+53645642	None:intergenic
TCCTACGTTAATACTCCCTC+CGG	0.570855	8_2:-53644790	Msa1255220:three_prime_UTR
TCAGATGCAGAATCAGAGAA+TGG	0.577226	8_2:-53644923	Msa1255220:CDS
TCTTCAGATAAACGTGTGGA+AGG	0.585551	8_2:+53645632	None:intergenic
GATTACACAAGATGAAACAT+TGG	0.592007	8_2:-53644854	Msa1255220:exon
GAATGGTCCTCGTGGCCCAA+AGG	0.595177	8_2:-53645420	Msa1255220:intron
TGCTTATAATAGTGACCGGA+GGG	0.599725	8_2:+53644775	None:intergenic
CATTATGCTGCTGTTAGTCG+CGG	0.600615	8_2:+53645039	None:intergenic
TCTCAATCCAAGTCAAGCAA+AGG	0.601871	8_2:-53645530	Msa1255220:CDS
ATGGAGTAGTGCAAGAGTAA+GGG	0.602866	8_2:+53645690	None:intergenic
CTGCCATACATTTGACTCTG+TGG	0.604113	8_2:+53644968	None:intergenic
ACGTGTGGAAGGGGTAGCAA+GGG	0.615165	8_2:+53645643	None:intergenic
AATTAGCAAGAGAAGAGCGA+CGG	0.619540	8_2:+53645594	None:intergenic
ATGGGAGGAACATGCACATG+TGG	0.624656	8_2:-53645791	Msa1255220:CDS
TTGCGGGATTAGATACAAGA+AGG	0.629096	8_2:-53645098	Msa1255220:CDS
TTGCTTATAATAGTGACCGG+AGG	0.646544	8_2:+53644774	None:intergenic
AGAGAGAAGAGCGAACGCTG+CGG	0.651764	8_2:-53645074	Msa1255220:CDS
ATGTTTACCTTTGGGCCACG+AGG	0.671239	8_2:+53645413	None:intergenic
CTTTGGAGGAATGGTCCTCG+TGG	0.675633	8_2:-53645428	Msa1255220:CDS
CAACAAGGTCACATAATGGG+AGG	0.680593	8_2:-53645806	Msa1255220:CDS
TTGGTCTTCAGATAAACGTG+TGG	0.689873	8_2:+53645628	None:intergenic
CGAGGACCATTCCTCCAAAG+GGG	0.705344	8_2:+53645431	None:intergenic
TGTTGTAGTAGCTCTCAACA+AGG	0.711133	8_2:-53645821	Msa1255220:CDS
ACCGGAGGGAGTATTAACGT+AGG	0.802024	8_2:+53644789	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTAAGAATGTTTTGTAATAA+TGG	-	chr8_2:53645521-53645540	Msa1255220:CDS	15.0%
!!!	TTAAGAATGTTTTGTAATAA+TGG	-	chr8_2:53645521-53645540	Msa1255220:CDS	15.0%
!!	AAAAACATTTATAGTCTATC+TGG	-	chr8_2:53645355-53645374	Msa1255220:intron	20.0%
!!	AAAAACATTTATAGTCTATC+TGG	-	chr8_2:53645355-53645374	Msa1255220:intron	20.0%
!	TATTATGTTGCAATGTTGTA+TGG	+	chr8_2:53644772-53644791	None:intergenic	25.0%
!	AATCATAATGATCATGTTGT+TGG	+	chr8_2:53644959-53644978	None:intergenic	25.0%
!	TTGAGAATTATGTTTAGTGT+TGG	+	chr8_2:53645123-53645142	None:intergenic	25.0%
!	TATTATGTTGCAATGTTGTA+TGG	+	chr8_2:53644772-53644791	None:intergenic	25.0%
!	AATCATAATGATCATGTTGT+TGG	+	chr8_2:53644959-53644978	None:intergenic	25.0%
!	TTGAGAATTATGTTTAGTGT+TGG	+	chr8_2:53645123-53645142	None:intergenic	25.0%
	TCAAGCAAAGGAAACAATAT+TGG	-	chr8_2:53645155-53645174	Msa1255220:intron	30.0%
	GGTAATGTATGTTTACCTTT+GGG	+	chr8_2:53645271-53645290	None:intergenic	30.0%
!	TGGTAATGTATGTTTACCTT+TGG	+	chr8_2:53645272-53645291	None:intergenic	30.0%
	AACATTTATAGTCTACTACG+AGG	+	chr8_2:53645330-53645349	None:intergenic	30.0%
	CGTAGTAGACTATAAATGTT+CGG	-	chr8_2:53645330-53645349	Msa1255220:intron	30.0%
	ACAACAACAACAACAATTCA+TGG	-	chr8_2:53645673-53645692	Msa1255220:CDS	30.0%
	GATTACACAAGATGAAACAT+TGG	-	chr8_2:53645819-53645838	Msa1255220:CDS	30.0%
!	GTTTTGCTTATAATAGTGAC+CGG	+	chr8_2:53645905-53645924	None:intergenic	30.0%
	TCAAGCAAAGGAAACAATAT+TGG	-	chr8_2:53645155-53645174	Msa1255220:intron	30.0%
	GGTAATGTATGTTTACCTTT+GGG	+	chr8_2:53645271-53645290	None:intergenic	30.0%
!	TGGTAATGTATGTTTACCTT+TGG	+	chr8_2:53645272-53645291	None:intergenic	30.0%
	AACATTTATAGTCTACTACG+AGG	+	chr8_2:53645330-53645349	None:intergenic	30.0%
	CGTAGTAGACTATAAATGTT+CGG	-	chr8_2:53645330-53645349	Msa1255220:intron	30.0%
	ACAACAACAACAACAATTCA+TGG	-	chr8_2:53645673-53645692	Msa1255220:CDS	30.0%
	GATTACACAAGATGAAACAT+TGG	-	chr8_2:53645819-53645838	Msa1255220:CDS	30.0%
!	GTTTTGCTTATAATAGTGAC+CGG	+	chr8_2:53645905-53645924	None:intergenic	30.0%
	CTACAACAATGATGCATCAT+TGG	+	chr8_2:53644840-53644859	None:intergenic	35.0%
	TCTCAACAAGGTCACATAAT+GGG	-	chr8_2:53644864-53644883	Msa1255220:CDS	35.0%
!!	GAGTAGTTAGAATTGGTTTG+AGG	+	chr8_2:53644918-53644937	None:intergenic	35.0%
	AAGCAAGGAGTAGTTAGAAT+TGG	+	chr8_2:53644925-53644944	None:intergenic	35.0%
!	TTGGTAGTTAGAGTAAAGCA+AGG	+	chr8_2:53644940-53644959	None:intergenic	35.0%
	ATTGTTTCCTTTGCTTGACT+TGG	+	chr8_2:53645153-53645172	None:intergenic	35.0%
	TTTGAACATAGACACGAAGA+TGG	+	chr8_2:53645292-53645311	None:intergenic	35.0%
!	TCTGACTTTGACTTTGTTAG+TGG	-	chr8_2:53645398-53645417	Msa1255220:intron	35.0%
!	CTGACTTTGACTTTGTTAGT+GGG	-	chr8_2:53645399-53645418	Msa1255220:intron	35.0%
	GCATTCCAAATTTCCTTTCT+TGG	-	chr8_2:53645772-53645791	Msa1255220:CDS	35.0%
	AGTCTCCAAGAAAGGAAATT+TGG	+	chr8_2:53645780-53645799	None:intergenic	35.0%
	TATGTTGCAATGTTGTATGG+AGG	+	chr8_2:53644769-53644788	None:intergenic	35.0%
	CTACAACAATGATGCATCAT+TGG	+	chr8_2:53644840-53644859	None:intergenic	35.0%
	TCTCAACAAGGTCACATAAT+GGG	-	chr8_2:53644864-53644883	Msa1255220:CDS	35.0%
!!	GAGTAGTTAGAATTGGTTTG+AGG	+	chr8_2:53644918-53644937	None:intergenic	35.0%
	AAGCAAGGAGTAGTTAGAAT+TGG	+	chr8_2:53644925-53644944	None:intergenic	35.0%
!	TTGGTAGTTAGAGTAAAGCA+AGG	+	chr8_2:53644940-53644959	None:intergenic	35.0%
	ATTGTTTCCTTTGCTTGACT+TGG	+	chr8_2:53645153-53645172	None:intergenic	35.0%
	TTTGAACATAGACACGAAGA+TGG	+	chr8_2:53645292-53645311	None:intergenic	35.0%
!	TCTGACTTTGACTTTGTTAG+TGG	-	chr8_2:53645398-53645417	Msa1255220:intron	35.0%
!	CTGACTTTGACTTTGTTAGT+GGG	-	chr8_2:53645399-53645418	Msa1255220:intron	35.0%
	GCATTCCAAATTTCCTTTCT+TGG	-	chr8_2:53645772-53645791	Msa1255220:CDS	35.0%
	AGTCTCCAAGAAAGGAAATT+TGG	+	chr8_2:53645780-53645799	None:intergenic	35.0%
	TGTTGTAGTAGCTCTCAACA+AGG	-	chr8_2:53644852-53644871	Msa1255220:exon	40.0%
	CTCTCAACAAGGTCACATAA+TGG	-	chr8_2:53644863-53644882	Msa1255220:CDS	40.0%
	ATGGAGTAGTGCAAGAGTAA+GGG	+	chr8_2:53644986-53645005	None:intergenic	40.0%
	GTGCAATCAACAGAAGAAGA+TGG	+	chr8_2:53645005-53645024	None:intergenic	40.0%
	TTCAGATAAACGTGTGGAAG+GGG	+	chr8_2:53645042-53645061	None:intergenic	40.0%
	CTTCAGATAAACGTGTGGAA+GGG	+	chr8_2:53645043-53645062	None:intergenic	40.0%
	TCTTCAGATAAACGTGTGGA+AGG	+	chr8_2:53645044-53645063	None:intergenic	40.0%
!	TTGGTCTTCAGATAAACGTG+TGG	+	chr8_2:53645048-53645067	None:intergenic	40.0%
	AATTAGCAAGAGAAGAGCGA+CGG	+	chr8_2:53645082-53645101	None:intergenic	40.0%
	TCTCAATCCAAGTCAAGCAA+AGG	-	chr8_2:53645143-53645162	Msa1255220:intron	40.0%
	TATAGTCTACTACGAGGTCT+CGG	+	chr8_2:53645324-53645343	None:intergenic	40.0%
	GTCAGACTCTTACTACTGAA+TGG	+	chr8_2:53645384-53645403	None:intergenic	40.0%
	TTGCGGGATTAGATACAAGA+AGG	-	chr8_2:53645575-53645594	Msa1255220:CDS	40.0%
	CAACCACAGAGTCAAATGTA+TGG	-	chr8_2:53645702-53645721	Msa1255220:CDS	40.0%
	TGGCAGTGAGCTAAATTTCA+TGG	-	chr8_2:53645722-53645741	Msa1255220:CDS	40.0%
	TCAGATGCAGAATCAGAGAA+TGG	-	chr8_2:53645750-53645769	Msa1255220:CDS	40.0%
	TTGTGTTGAGTCTCCAAGAA+AGG	+	chr8_2:53645788-53645807	None:intergenic	40.0%
	TGCTTATAATAGTGACCGGA+GGG	+	chr8_2:53645901-53645920	None:intergenic	40.0%
	TTGCTTATAATAGTGACCGG+AGG	+	chr8_2:53645902-53645921	None:intergenic	40.0%
	TGTTGTAGTAGCTCTCAACA+AGG	-	chr8_2:53644852-53644871	Msa1255220:exon	40.0%
	CTCTCAACAAGGTCACATAA+TGG	-	chr8_2:53644863-53644882	Msa1255220:CDS	40.0%
	ATGGAGTAGTGCAAGAGTAA+GGG	+	chr8_2:53644986-53645005	None:intergenic	40.0%
	GTGCAATCAACAGAAGAAGA+TGG	+	chr8_2:53645005-53645024	None:intergenic	40.0%
	TTCAGATAAACGTGTGGAAG+GGG	+	chr8_2:53645042-53645061	None:intergenic	40.0%
	CTTCAGATAAACGTGTGGAA+GGG	+	chr8_2:53645043-53645062	None:intergenic	40.0%
	TCTTCAGATAAACGTGTGGA+AGG	+	chr8_2:53645044-53645063	None:intergenic	40.0%
!	TTGGTCTTCAGATAAACGTG+TGG	+	chr8_2:53645048-53645067	None:intergenic	40.0%
	AATTAGCAAGAGAAGAGCGA+CGG	+	chr8_2:53645082-53645101	None:intergenic	40.0%
	TCTCAATCCAAGTCAAGCAA+AGG	-	chr8_2:53645143-53645162	Msa1255220:intron	40.0%
	TATAGTCTACTACGAGGTCT+CGG	+	chr8_2:53645324-53645343	None:intergenic	40.0%
	GTCAGACTCTTACTACTGAA+TGG	+	chr8_2:53645384-53645403	None:intergenic	40.0%
	TTGCGGGATTAGATACAAGA+AGG	-	chr8_2:53645575-53645594	Msa1255220:CDS	40.0%
	CAACCACAGAGTCAAATGTA+TGG	-	chr8_2:53645702-53645721	Msa1255220:CDS	40.0%
	TGGCAGTGAGCTAAATTTCA+TGG	-	chr8_2:53645722-53645741	Msa1255220:CDS	40.0%
	TCAGATGCAGAATCAGAGAA+TGG	-	chr8_2:53645750-53645769	Msa1255220:CDS	40.0%
	TTGTGTTGAGTCTCCAAGAA+AGG	+	chr8_2:53645788-53645807	None:intergenic	40.0%
	TGCTTATAATAGTGACCGGA+GGG	+	chr8_2:53645901-53645920	None:intergenic	40.0%
	TTGCTTATAATAGTGACCGG+AGG	+	chr8_2:53645902-53645921	None:intergenic	40.0%
!	CAACAAGGTCACATAATGGG+AGG	-	chr8_2:53644867-53644886	Msa1255220:CDS	45.0%
	GATGGAGTAGTGCAAGAGTA+AGG	+	chr8_2:53644987-53645006	None:intergenic	45.0%
	AGCGACGGTTTCGTTTATCT+TGG	+	chr8_2:53645067-53645086	None:intergenic	45.0%
	CAGTCACTATGCAATGCTTG+CGG	-	chr8_2:53645558-53645577	Msa1255220:CDS	45.0%
	AGTCACTATGCAATGCTTGC+GGG	-	chr8_2:53645559-53645578	Msa1255220:CDS	45.0%
	CATTATGCTGCTGTTAGTCG+CGG	+	chr8_2:53645637-53645656	None:intergenic	45.0%
!	AACAGCAGCATAATGGAGTC+AGG	-	chr8_2:53645642-53645661	Msa1255220:CDS	45.0%
	CTGCCATACATTTGACTCTG+TGG	+	chr8_2:53645708-53645727	None:intergenic	45.0%
	TCCTACGTTAATACTCCCTC+CGG	-	chr8_2:53645883-53645902	Msa1255220:five_prime_UTR	45.0%
!	CAACAAGGTCACATAATGGG+AGG	-	chr8_2:53644867-53644886	Msa1255220:CDS	45.0%
	GATGGAGTAGTGCAAGAGTA+AGG	+	chr8_2:53644987-53645006	None:intergenic	45.0%
	AGCGACGGTTTCGTTTATCT+TGG	+	chr8_2:53645067-53645086	None:intergenic	45.0%
	CAGTCACTATGCAATGCTTG+CGG	-	chr8_2:53645558-53645577	Msa1255220:CDS	45.0%
	AGTCACTATGCAATGCTTGC+GGG	-	chr8_2:53645559-53645578	Msa1255220:CDS	45.0%
	CATTATGCTGCTGTTAGTCG+CGG	+	chr8_2:53645637-53645656	None:intergenic	45.0%
!	AACAGCAGCATAATGGAGTC+AGG	-	chr8_2:53645642-53645661	Msa1255220:CDS	45.0%
	CTGCCATACATTTGACTCTG+TGG	+	chr8_2:53645708-53645727	None:intergenic	45.0%
	TCCTACGTTAATACTCCCTC+CGG	-	chr8_2:53645883-53645902	Msa1255220:five_prime_UTR	45.0%
!!	CAAAAAATTATTATTAATAA+CGG	+	chr8_2:53645464-53645483	None:intergenic	5.0%
!!	CAAAAAATTATTATTAATAA+CGG	+	chr8_2:53645464-53645483	None:intergenic	5.0%
	ATGGGAGGAACATGCACATG+TGG	-	chr8_2:53644882-53644901	Msa1255220:CDS	50.0%
	ACACCACTTCTACACCCCTT+TGG	-	chr8_2:53645228-53645247	Msa1255220:intron	50.0%
!	TCTACACCCCTTTGGAGGAA+TGG	-	chr8_2:53645236-53645255	Msa1255220:intron	50.0%
	ACGAGGACCATTCCTCCAAA+GGG	+	chr8_2:53645246-53645265	None:intergenic	50.0%
	ATGTTTACCTTTGGGCCACG+AGG	+	chr8_2:53645263-53645282	None:intergenic	50.0%
	CGCGACTAACAGCAGCATAA+TGG	-	chr8_2:53645635-53645654	Msa1255220:CDS	50.0%
!!	ACCGGAGGGAGTATTAACGT+AGG	+	chr8_2:53645887-53645906	None:intergenic	50.0%
	ATGGGAGGAACATGCACATG+TGG	-	chr8_2:53644882-53644901	Msa1255220:CDS	50.0%
	ACACCACTTCTACACCCCTT+TGG	-	chr8_2:53645228-53645247	Msa1255220:intron	50.0%
!	TCTACACCCCTTTGGAGGAA+TGG	-	chr8_2:53645236-53645255	Msa1255220:intron	50.0%
	ACGAGGACCATTCCTCCAAA+GGG	+	chr8_2:53645246-53645265	None:intergenic	50.0%
	ATGTTTACCTTTGGGCCACG+AGG	+	chr8_2:53645263-53645282	None:intergenic	50.0%
	CGCGACTAACAGCAGCATAA+TGG	-	chr8_2:53645635-53645654	Msa1255220:CDS	50.0%
!!	ACCGGAGGGAGTATTAACGT+AGG	+	chr8_2:53645887-53645906	None:intergenic	50.0%
	ACGTGTGGAAGGGGTAGCAA+GGG	+	chr8_2:53645033-53645052	None:intergenic	55.0%
	AACGTGTGGAAGGGGTAGCA+AGG	+	chr8_2:53645034-53645053	None:intergenic	55.0%
!	CCTCCAAAGGGGTGTAGAAG+TGG	+	chr8_2:53645234-53645253	None:intergenic	55.0%
	CCACTTCTACACCCCTTTGG+AGG	-	chr8_2:53645231-53645250	Msa1255220:intron	55.0%
	CGAGGACCATTCCTCCAAAG+GGG	+	chr8_2:53645245-53645264	None:intergenic	55.0%
	CACGAGGACCATTCCTCCAA+AGG	+	chr8_2:53645247-53645266	None:intergenic	55.0%
!	CTTTGGAGGAATGGTCCTCG+TGG	-	chr8_2:53645245-53645264	Msa1255220:intron	55.0%
	AGAGAGAAGAGCGAACGCTG+CGG	-	chr8_2:53645599-53645618	Msa1255220:CDS	55.0%
	ACGTGTGGAAGGGGTAGCAA+GGG	+	chr8_2:53645033-53645052	None:intergenic	55.0%
	AACGTGTGGAAGGGGTAGCA+AGG	+	chr8_2:53645034-53645053	None:intergenic	55.0%
!	CCTCCAAAGGGGTGTAGAAG+TGG	+	chr8_2:53645234-53645253	None:intergenic	55.0%
	CCACTTCTACACCCCTTTGG+AGG	-	chr8_2:53645231-53645250	Msa1255220:intron	55.0%
	CGAGGACCATTCCTCCAAAG+GGG	+	chr8_2:53645245-53645264	None:intergenic	55.0%
	CACGAGGACCATTCCTCCAA+AGG	+	chr8_2:53645247-53645266	None:intergenic	55.0%
!	CTTTGGAGGAATGGTCCTCG+TGG	-	chr8_2:53645245-53645264	Msa1255220:intron	55.0%
	AGAGAGAAGAGCGAACGCTG+CGG	-	chr8_2:53645599-53645618	Msa1255220:CDS	55.0%
	GAATGGTCCTCGTGGCCCAA+AGG	-	chr8_2:53645253-53645272	Msa1255220:intron	60.0%
	GCTGTTAGTCGCGGCTGTAG+TGG	+	chr8_2:53645628-53645647	None:intergenic	60.0%
	GAATGGTCCTCGTGGCCCAA+AGG	-	chr8_2:53645253-53645272	Msa1255220:intron	60.0%
	GCTGTTAGTCGCGGCTGTAG+TGG	+	chr8_2:53645628-53645647	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	53644768	53645927	53644768	ID=Msa1255220;Name=Msa1255220
chr8_2	mRNA	53644768	53645927	53644768	ID=Msa1255220-mRNA-1;Parent=Msa1255220;Name=Msa1255220-mRNA-1;_AED=0.00;_eAED=0.00;_QI=72\|1\|1\|1\|1\|1\|2\|94\|235
chr8_2	exon	53644768	53645134	53644768	ID=Msa1255220-mRNA-1:exon:16622;Parent=Msa1255220-mRNA-1
chr8_2	exon	53645421	53645927	53645421	ID=Msa1255220-mRNA-1:exon:16621;Parent=Msa1255220-mRNA-1
chr8_2	five_prime_UTR	53645856	53645927	53645856	ID=Msa1255220-mRNA-1:five_prime_utr;Parent=Msa1255220-mRNA-1
chr8_2	CDS	53645421	53645855	53645421	ID=Msa1255220-mRNA-1:cds;Parent=Msa1255220-mRNA-1
chr8_2	CDS	53644862	53645134	53644862	ID=Msa1255220-mRNA-1:cds;Parent=Msa1255220-mRNA-1
chr8_2	three_prime_UTR	53644768	53644861	53644768	ID=Msa1255220-mRNA-1:three_prime_utr;Parent=Msa1255220-mRNA-1

Gene Sequence

>Msa1255220

ATGATGCATCATTGTTGTAGTAGCTCTCAACAAGGTCACATAATGGGAGGAACATGCACATGTGGTATGTTTCATCCTCAAACCAATTCTAACTACTCCTTGCTTTACTCTAACTACCAACAACATGATCATTATGATTTTGACCCTTACTCTTGCACTACTCCATCTTCTTCTGTTGATTGCACACTCTCCCTTGCTACCCCTTCCACACGTTTATCTGAAGACCAAGATAAACGAAACCGTCGCTCTTCTCTTGCTAATTTTTTTTGTAGCAAATCTTCCAACACTAAACATAATTCTCAATCTCAATCCAAGTCAAGCAAAGGAAACAATATTGGTAGTAACTCTAACAATGATTCTCTTCTTTCTCGTCGATGTGCTAGCTGTGACACCACTTCTACACCCCTTTGGAGGAATGGTCCTCGTGGCCCAAAGTCACTATGCAATGCTTGCGGGATTAGATACAAGAAGGAAGAGAGAAGAGCGAACGCTGCGGTTGCCACTACAGCCGCGACTAACAGCAGCATAATGGAGTCAGGAAACTTCTACAACAACAACAACAATTCATGGTACTCACAACCACAGAGTCAAATGTATGGCAGTGAGCTAAATTTCATGGATGATTCAGATGCAGAATCAGAGAATGGCATTCCAAATTTCCTTTCTTGGAGACTCAACACAAGTCTTGTTCACGATTACACAAGATGA

Protein sequence

>Msa1255220

MMHHCCSSSQQGHIMGGTCTCGMFHPQTNSNYSLLYSNYQQHDHYDFDPYSCTTPSSSVDCTLSLATPSTRLSEDQDKRNRRSSLANFFCSKSSNTKHNSQSQSKSSKGNNIGSNSNNDSLLSRRCASCDTTSTPLWRNGPRGPKSLCNACGIRYKKEERRANAAVATTAATNSSIMESGNFYNNNNNSWYSQPQSQMYGSELNFMDDSDAESENGIPNFLSWRLNTSLVHDYTR*