AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0577060

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0577060 MtrunA17_Chr8g0384731 92.431 436 8 2 1 412 1 435 0.0 818
Msa0577060 MtrunA17_Chr3g0130741 67.290 428 100 4 24 412 19 445 0.0 576
Msa0577060 MtrunA17_Chr1g0159791 51.444 381 154 6 54 406 37 414 2.57e-128 376
Msa0577060 MtrunA17_Chr4g0010951 45.606 421 166 9 47 407 34 451 2.94e-115 344
Msa0577060 MtrunA17_Chr6g0484731 43.158 380 174 10 59 401 176 550 4.06e-89 280
Msa0577060 MtrunA17_Chr3g0078321 37.306 386 195 14 59 408 45 419 8.02e-76 241
Msa0577060 MtrunA17_Chr4g0010941 47.881 236 92 3 199 405 2 235 6.56e-72 225
Msa0577060 MtrunA17_Chr7g0221681 27.230 426 231 15 39 409 40 441 3.17e-29 119
Msa0577060 MtrunA17_Chr7g0249451 26.914 405 222 15 59 409 54 438 3.22e-28 115
Msa0577060 MtrunA17_Chr4g0053721 27.174 368 219 14 73 404 79 433 2.21e-26 111
Msa0577060 MtrunA17_Chr1g0179751 23.174 397 224 14 73 409 107 482 2.41e-26 110
Msa0577060 MtrunA17_Chr2g0320621 25.130 386 216 15 70 402 73 438 8.23e-26 108
Msa0577060 MtrunA17_Chr5g0401601 26.579 380 222 15 65 404 79 441 1.50e-25 108
Msa0577060 MtrunA17_Chr7g0265331 23.650 389 217 14 70 402 77 441 1.97e-24 105
Msa0577060 MtrunA17_Chr5g0406001 27.226 393 214 17 63 408 106 473 3.23e-24 104
Msa0577060 MtrunA17_Chr4g0027681 24.607 382 216 15 73 402 76 437 5.45e-24 103
Msa0577060 MtrunA17_Chr8g0371371 26.794 418 241 18 37 411 78 473 1.43e-22 99.8
Msa0577060 MtrunA17_Chr1g0189381 25.000 404 232 16 59 409 52 437 1.01e-21 97.1
Msa0577060 MtrunA17_Chr8g0352671 24.936 393 213 14 60 404 81 439 1.14e-21 96.7
Msa0577060 MtrunA17_Chr8g0386441 25.127 394 226 14 54 404 65 432 1.75e-21 95.9
Msa0577060 MtrunA17_Chr5g0425141 24.548 387 231 14 66 411 94 460 6.37e-21 94.7
Msa0577060 MtrunA17_Chr2g0333991 23.592 373 217 14 72 400 86 434 9.54e-20 90.9
Msa0577060 MtrunA17_Chr2g0311451 24.332 374 215 14 72 401 86 435 9.87e-20 90.9
Msa0577060 MtrunA17_Chr3g0124421 24.510 408 227 17 48 404 76 453 7.58e-19 88.6
Msa0577060 MtrunA17_Chr8g0371351 24.760 416 239 16 35 404 65 452 2.25e-18 87.0
Msa0577060 MtrunA17_Chr3g0130931 26.817 399 200 24 59 404 92 451 7.43e-18 85.1
Msa0577060 MtrunA17_Chr8g0352661 25.000 388 222 15 60 404 78 439 7.63e-18 85.1
Msa0577060 MtrunA17_Chr2g0307991 25.000 376 202 15 75 401 60 404 8.91e-18 84.7
Msa0577060 MtrunA17_Chr2g0310831 24.872 390 222 18 54 401 80 440 1.03e-17 84.7
Msa0577060 MtrunA17_Chr5g0419291 25.737 373 204 17 73 401 85 428 2.86e-17 83.2
Msa0577060 MtrunA17_Chr5g0395911 24.603 378 217 15 68 402 62 414 2.50e-15 77.4
Msa0577060 MtrunA17_Chr2g0310821 24.566 403 233 17 38 402 74 443 3.13e-15 77.0
Msa0577060 MtrunA17_Chr2g0308001 25.258 388 197 18 75 404 59 411 3.73e-15 77.0
Msa0577060 MtrunA17_Chr3g0099891 26.341 410 231 22 35 401 73 454 1.44e-14 75.1
Msa0577060 MtrunA17_Chr5g0442921 26.087 391 206 18 73 404 190 556 1.70e-14 75.1
Msa0577060 MtrunA17_Chr1g0179091 23.077 364 236 12 51 401 34 366 4.07e-14 73.2
Msa0577060 MtrunA17_Chr3g0110191 24.074 378 216 14 77 406 89 443 4.59e-14 73.6
Msa0577060 MtrunA17_Chr6g0484901 25.201 373 195 18 75 401 157 491 8.11e-14 73.2
Msa0577060 MtrunA17_Chr3g0087851 23.256 387 213 15 70 404 85 439 1.23e-13 72.4
Msa0577060 MtrunA17_Chr1g0165201 24.865 370 210 14 73 401 127 469 1.59e-13 72.0
Msa0577060 MtrunA17_Chr3g0099901 25.867 375 216 15 67 402 135 486 1.81e-13 72.0
Msa0577060 MtrunA17_Chr5g0434411 22.917 384 217 12 77 406 84 442 2.08e-13 71.6
Msa0577060 MtrunA17_Chr5g0415951 24.672 381 221 18 61 401 80 434 3.57e-13 70.9
Msa0577060 MtrunA17_Chr1g0212321 24.202 376 204 15 73 401 145 486 8.07e-13 70.1
Msa0577060 MtrunA17_Chr2g0291201 24.211 380 210 17 73 402 101 452 1.14e-12 69.3
Msa0577060 MtrunA17_Chr4g0023351 32.022 178 87 8 59 209 46 216 1.25e-12 68.2
Msa0577060 MtrunA17_Chr6g0486981 22.427 379 217 15 75 404 89 439 1.61e-12 68.9
Msa0577060 MtrunA17_Chr5g0395931 23.797 374 226 14 68 402 83 436 1.82e-12 68.6
Msa0577060 MtrunA17_Chr3g0095891 24.130 431 246 20 32 404 54 461 1.85e-12 68.6
Msa0577060 MtrunA17_Chr7g0275731 25.240 416 234 19 32 402 102 485 5.06e-12 67.4
Msa0577060 MtrunA17_Chr8g0390761 28.571 168 77 6 77 216 119 271 2.61e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0577060 AT4G33490.2 58.095 420 137 7 11 404 8 414 6.07e-167 475
Msa0577060 AT4G33490.1 58.163 392 125 7 11 376 5 383 8.56e-152 436
Msa0577060 AT1G44130.1 50.263 380 157 8 56 407 30 405 9.92e-131 382
Msa0577060 AT1G77480.2 47.301 389 177 5 47 408 39 426 3.08e-124 367
Msa0577060 AT1G77480.1 47.150 386 176 5 47 405 39 423 1.15e-122 363
Msa0577060 AT1G49050.1 42.377 387 174 13 59 401 187 568 2.20e-85 271
Msa0577060 AT1G49050.2 41.892 370 170 12 74 401 29 395 1.70e-80 254
Msa0577060 AT1G49050.3 40.293 273 122 9 59 292 187 457 1.49e-51 179
Msa0577060 AT1G65240.1 26.750 400 223 14 59 409 56 434 3.06e-33 130
Msa0577060 AT5G43100.2 28.307 378 210 15 65 404 66 420 2.10e-32 128
Msa0577060 AT5G43100.1 28.307 378 210 15 65 404 66 420 8.03e-32 127
Msa0577060 AT3G02740.1 27.387 398 225 12 59 409 67 447 1.73e-31 125
Msa0577060 AT2G36670.2 25.815 399 225 13 56 402 79 458 3.34e-31 125
Msa0577060 AT1G05840.1 26.355 406 223 13 59 409 62 446 7.44e-30 120
Msa0577060 AT3G02740.2 26.684 386 221 11 69 409 4 372 3.08e-29 118
Msa0577060 AT5G22850.1 25.917 409 232 14 48 403 52 442 3.31e-29 119
Msa0577060 AT2G36670.4 25.587 383 208 12 74 402 33 392 3.81e-29 118
Msa0577060 AT2G36670.3 25.587 383 208 12 74 402 33 392 3.81e-29 118
Msa0577060 AT2G36670.1 25.495 404 225 14 56 402 79 463 4.04e-29 119
Msa0577060 AT5G36260.1 25.666 413 244 12 46 411 47 443 1.84e-28 117
Msa0577060 AT1G08210.4 25.185 405 227 13 52 402 93 475 4.78e-27 113
Msa0577060 AT1G08210.3 25.185 405 227 13 52 402 107 489 5.29e-27 113
Msa0577060 AT1G08210.1 25.185 405 227 13 52 402 59 441 5.44e-27 112
Msa0577060 AT3G50050.1 27.224 371 225 14 65 404 83 439 2.15e-25 108
Msa0577060 AT1G08210.2 25.000 432 221 17 52 402 59 468 8.37e-23 100
Msa0577060 AT3G25700.1 25.455 385 214 16 73 401 82 449 1.32e-21 96.7
Msa0577060 AT2G17760.1 24.752 404 242 15 37 401 69 449 1.39e-21 97.1
Msa0577060 AT5G10080.1 25.946 370 209 12 79 402 104 454 2.33e-21 96.7
Msa0577060 AT3G51330.3 26.260 377 212 17 72 402 98 454 4.26e-20 92.4
Msa0577060 AT3G51350.1 27.200 375 203 17 74 401 101 452 4.37e-20 92.8
Msa0577060 AT4G35880.1 27.105 380 214 17 69 407 100 457 4.40e-20 92.8
Msa0577060 AT3G51330.1 26.330 376 211 17 72 401 98 453 5.17e-20 92.4
Msa0577060 AT2G28010.1 26.371 383 227 18 47 404 42 394 6.83e-20 91.3
Msa0577060 AT2G28030.1 23.467 375 236 15 54 404 43 390 8.66e-18 85.1
Msa0577060 AT2G28040.2 25.130 386 230 17 45 404 32 384 1.52e-16 81.3
Msa0577060 AT2G28040.1 25.130 386 230 17 45 404 41 393 1.94e-16 80.9
Msa0577060 AT3G51340.1 26.121 379 213 16 72 401 99 459 2.15e-16 81.6
Msa0577060 AT3G51340.2 25.401 374 210 15 72 401 99 447 6.58e-16 80.1
Msa0577060 AT2G03200.1 23.798 416 222 20 45 404 85 461 1.55e-15 78.6
Msa0577060 AT1G66180.1 22.572 381 217 14 77 404 74 429 4.12e-15 77.0
Msa0577060 AT1G01300.1 27.005 374 199 17 73 401 140 484 2.44e-14 75.1
Msa0577060 AT4G35880.2 26.648 349 193 17 69 376 100 426 2.69e-14 74.7
Msa0577060 AT3G59080.2 24.359 390 239 15 44 404 137 499 2.69e-14 75.1
Msa0577060 AT3G51330.2 25.641 351 195 17 72 376 98 428 9.04e-14 73.2
Msa0577060 AT3G51360.7 22.164 379 219 16 72 402 2 352 2.69e-13 71.6
Msa0577060 AT3G51360.9 22.951 366 226 14 74 402 100 446 3.87e-13 71.2
Msa0577060 AT3G51360.1 22.951 366 226 14 74 402 90 436 4.83e-13 70.9
Msa0577060 AT3G18490.1 23.925 372 209 19 73 401 160 500 1.59e-12 69.3
Msa0577060 AT3G20015.1 24.865 370 209 16 73 401 129 470 3.43e-12 68.2
Msa0577060 AT2G42980.1 24.742 388 218 14 73 404 158 527 5.27e-12 67.8
Msa0577060 AT3G59080.1 23.389 419 243 15 44 404 137 535 7.68e-12 67.4
Msa0577060 AT3G51340.3 26.820 261 148 9 79 300 107 363 1.98e-11 65.9
Msa0577060 AT3G51340.4 26.523 279 157 11 79 316 107 378 2.12e-11 65.5

Find 148 sgRNAs with CRISPR-Local

Find 263 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATTGTGATCCCTATTTA+TGG 0.123424 4_1:+81223411 Msa0577060:CDS
AAACTGAAATCTCTGGATTT+TGG 0.144007 4_1:-81226966 None:intergenic
AGATAGGTAGGGTGGGATTA+AGG 0.217206 4_1:+81223238 Msa0577060:CDS
TTTCTAAATGCAGCTGAATA+AGG 0.250514 4_1:+81226221 Msa0577060:intron
GGATAAACGTTGCCATAAAT+AGG 0.257987 4_1:-81223423 None:intergenic
AACGAATCGATTGATGAAAT+TGG 0.264253 4_1:-81225380 None:intergenic
ATTTCATAGTCACATTGATT+AGG 0.289901 4_1:-81224569 None:intergenic
CACCCTTTAGATGGAATACT+TGG 0.291237 4_1:+81225212 Msa0577060:CDS
TTACCAAGCAATGATTTCTT+TGG 0.294065 4_1:+81225623 Msa0577060:CDS
AGTGTCAACATCAAGAAAAT+AGG 0.297686 4_1:-81224076 None:intergenic
TTTCATAGTCACATTGATTA+GGG 0.312831 4_1:-81224568 None:intergenic
AGCATTGTGTAGAGTATATT+GGG 0.314014 4_1:+81227142 Msa0577060:three_prime_UTR
TCAACTTACCACCCTTTAGA+TGG 0.325308 4_1:+81225203 Msa0577060:CDS
CTAAATTCTCACCTCCAATT+AGG 0.327781 4_1:-81226602 None:intergenic
AAGCATTGTGTAGAGTATAT+TGG 0.329838 4_1:+81227141 Msa0577060:three_prime_UTR
TTGGGTGAACTGAACCTAAT+TGG 0.334057 4_1:+81226588 Msa0577060:CDS
TCTTTAAAAGTACCTAATTA+AGG 0.339236 4_1:+81226993 Msa0577060:three_prime_UTR
TTTAAATTATTGATTCTTGT+TGG 0.351690 4_1:-81223078 None:intergenic
GTCATATGCATGCCTTAATT+AGG 0.358204 4_1:-81227005 None:intergenic
TCTTCTTGTTCAGCTTGGTT+TGG 0.372917 4_1:+81223300 Msa0577060:CDS
GATAGGTAGGGTGGGATTAA+GGG 0.398450 4_1:+81223239 Msa0577060:CDS
AGATCATAAACTGAAATCTC+TGG 0.399104 4_1:-81226973 None:intergenic
TGAATCATTAGATTATTTCC+TGG 0.405081 4_1:-81222783 None:intergenic
AGTTCACCCAATCCTACTTC+AGG 0.414059 4_1:-81226576 None:intergenic
GGAGCAGGAAAGAAGTTTCC+AGG 0.414558 4_1:+81222765 None:intergenic
AAGTTGAAGGTGAAAATATC+TGG 0.417456 4_1:-81225187 None:intergenic
GACAACGAGAAGCAGTTGAT+TGG 0.418942 4_1:+81226915 Msa0577060:CDS
GTGCCATTCTAACTTTAAGT+TGG 0.419171 4_1:-81224667 None:intergenic
AGAAGAAGATGAAGATAGGT+AGG 0.420924 4_1:+81223226 Msa0577060:exon
GATAAACGTTGCCATAAATA+GGG 0.423110 4_1:-81223422 None:intergenic
ATGGCAACGTTTATCCTGTT+GGG 0.427167 4_1:+81223430 Msa0577060:CDS
GTGTCAACATCAAGAAAATA+GGG 0.431013 4_1:-81224075 None:intergenic
ATAGGTAAAGTCTGGTCATC+AGG 0.433183 4_1:-81226273 None:intergenic
AACAAGAAGAAGATGAAGAT+AGG 0.439947 4_1:+81223222 Msa0577060:exon
TTAAATATTATCTTTGACAC+TGG 0.441413 4_1:+81225570 Msa0577060:CDS
ACTTGTCTTCTTGTTCAGCT+TGG 0.441843 4_1:+81223295 Msa0577060:CDS
GAAGATGAAGATAGGTAGGG+TGG 0.442669 4_1:+81223230 Msa0577060:exon
CTGAGAGTTGAAGTATGTAT+AGG 0.443665 4_1:-81225598 None:intergenic
GATATTATAAGATAAGCTTC+AGG 0.447328 4_1:-81226411 None:intergenic
TGAAAGAAATTGAAGGATAA+TGG 0.452938 4_1:-81223119 None:intergenic
GATATCACAGTTGAATAGTC+AGG 0.453876 4_1:+81225259 Msa0577060:CDS
AAGTTGAAAAGTTGTAGAAA+AGG 0.460593 4_1:-81222888 None:intergenic
TTGGTGAAACTAAGTGTTAG+TGG 0.461386 4_1:-81226354 None:intergenic
AGTCCAAGTATTCCATCTAA+AGG 0.461510 4_1:-81225215 None:intergenic
GGTGGGATTAAGGGTGATGA+TGG 0.463114 4_1:+81223248 Msa0577060:CDS
AACTTTAAGTTGGACTCCAT+TGG 0.463263 4_1:-81224657 None:intergenic
TATGGCAACGTTTATCCTGT+TGG 0.466319 4_1:+81223429 Msa0577060:CDS
GTTATTTGTTGCAAGAATAT+GGG 0.467337 4_1:+81226525 Msa0577060:intron
TTAGATGGAATACTTGGACT+TGG 0.471121 4_1:+81225218 Msa0577060:CDS
TGTACTAGTTCATGAAGAAA+AGG 0.472388 4_1:-81223044 None:intergenic
GAACGGCCCTGAAGTAGGAT+TGG 0.472405 4_1:+81226569 Msa0577060:CDS
GGAATACTATTGCAATCTGC+AGG 0.473909 4_1:-81226945 None:intergenic
GTCCAAGTATTCCATCTAAA+GGG 0.477058 4_1:-81225214 None:intergenic
ATTAATGCATATTATAGAGC+AGG 0.483123 4_1:+81227054 Msa0577060:three_prime_UTR
ATCGTATCACGTTAATTTGA+CGG 0.486448 4_1:+81227229 Msa0577060:three_prime_UTR
TAATATTACCTCAGAACATT+GGG 0.489036 4_1:-81224139 None:intergenic
TCCTCTATGATCTATAGTAA+TGG 0.492324 4_1:-81223197 None:intergenic
ACATCAAGAAAATAGGGCCT+TGG 0.492843 4_1:-81224069 None:intergenic
AATGAAATATCATACCCAAC+AGG 0.493100 4_1:-81223444 None:intergenic
GGAGGAAGGAAGACCGGGGT+TGG 0.493414 4_1:+81225543 Msa0577060:CDS
ACCTCAGAACATTGGGTACA+AGG 0.494290 4_1:-81224132 None:intergenic
CTTAAAGTTAGAATGGCACT+TGG 0.502205 4_1:+81224671 Msa0577060:CDS
GCCAGCACATAGGTAAAGTC+TGG 0.502564 4_1:-81226281 None:intergenic
AAGATGAAGATAGGTAGGGT+GGG 0.502567 4_1:+81223231 Msa0577060:exon
AACGGCCCTGAAGTAGGATT+GGG 0.503963 4_1:+81226570 Msa0577060:CDS
GTTCACCCAATCCTACTTCA+GGG 0.504181 4_1:-81226575 None:intergenic
ATGATTCTTCTCGAATGAGT+TGG 0.504399 4_1:+81225354 Msa0577060:CDS
TACCATCACTTTGTCTAGCA+TGG 0.505530 4_1:-81226889 None:intergenic
CATACCTTGTGAGTAAGAAA+AGG 0.506186 4_1:+81222959 Msa0577060:five_prime_UTR
ACAGTTGAATAGTCAGGGCT+TGG 0.506595 4_1:+81225265 Msa0577060:CDS
ATTGGGGTAAGCTATTATGC+AGG 0.506850 4_1:+81227159 Msa0577060:three_prime_UTR
AGGTTTAACTCTTCCACCAT+TGG 0.508559 4_1:-81226373 None:intergenic
AGTCCAACTTAAAGTTAGAA+TGG 0.510476 4_1:+81224664 Msa0577060:CDS
TCAAGAAAATAGGGCCTTGG+TGG 0.511612 4_1:-81224066 None:intergenic
TACCTTCTCAACTTCACCAA+TGG 0.512014 4_1:+81224641 Msa0577060:CDS
ACGAGAAGCAGTTGATTGGA+TGG 0.515480 4_1:+81226919 Msa0577060:CDS
CTATACATACTTCAACTCTC+AGG 0.516576 4_1:+81225599 Msa0577060:CDS
TGTTTAAGCTCGCGAGGAGG+GGG 0.519451 4_1:+81225308 Msa0577060:CDS
TGGGAGAGCGTGTGAATGAA+TGG 0.519940 4_1:-81222834 None:intergenic
ATGGAATACTTGGACTTGGA+AGG 0.520338 4_1:+81225222 Msa0577060:CDS
TTTGGTAATAAACGCAAAAG+TGG 0.521006 4_1:+81223318 Msa0577060:CDS
ATTCTGAACGGCCCTGAAGT+AGG 0.523129 4_1:+81226564 Msa0577060:CDS
TGGTGAATGAGCAAGTGTTA+AGG 0.528403 4_1:-81222814 None:intergenic
CTTGAAGACATGGCTTCACC+TGG 0.531998 4_1:-81223357 None:intergenic
TCTAAAGGGTGGTAAGTTGA+AGG 0.532635 4_1:-81225200 None:intergenic
TGGAATACTTGGACTTGGAA+GGG 0.533058 4_1:+81225223 Msa0577060:CDS
TTCACATGTGTAGTCATCGG+TGG 0.533364 4_1:-81224543 None:intergenic
GAAAATAGGGCCTTGGTGGT+TGG 0.533650 4_1:-81224062 None:intergenic
GGGCTTGGTGCGAAATGTGA+TGG 0.536228 4_1:+81225280 Msa0577060:CDS
CGAGAAGCAGTTGATTGGAT+GGG 0.543766 4_1:+81226920 Msa0577060:CDS
AGGGGAAAACATTACTCTGC+AGG 0.544182 4_1:+81225501 Msa0577060:intron
TGATTCTTGTTGGAAAGAGT+TGG 0.546273 4_1:-81223068 None:intergenic
AATATGGGAAATGTTTGTCT+AGG 0.546632 4_1:+81226540 Msa0577060:CDS
ACCAGACTTTACCTATGTGC+TGG 0.546884 4_1:+81226280 Msa0577060:CDS
CTCCATTGGTGAAGTTGAGA+AGG 0.547745 4_1:-81224643 None:intergenic
ATAAACTCAACATTAGAAAG+TGG 0.548243 4_1:-81223015 None:intergenic
GGAATACTTGGACTTGGAAG+GGG 0.550065 4_1:+81225224 Msa0577060:CDS
GTAATATTACCTCAGAACAT+TGG 0.551100 4_1:-81224140 None:intergenic
AGAAATTGAAGGATAATGGT+GGG 0.551649 4_1:-81223115 None:intergenic
ACACTTAGTTTCACCAATGG+TGG 0.552466 4_1:+81226360 Msa0577060:CDS
ATAAATAGGGATCACAATTG+AGG 0.553125 4_1:-81223409 None:intergenic
AGTCTCATGAACCATGCTGC+TGG 0.557344 4_1:+81223384 Msa0577060:CDS
TCAGGAGGGATCTCAAACTG+AGG 0.558793 4_1:-81226393 None:intergenic
GAAGAAGATGAAGATAGGTA+GGG 0.561089 4_1:+81223227 Msa0577060:exon
GTCTTCACATGTGTAGTCAT+CGG 0.562506 4_1:-81224546 None:intergenic
GCTGATCAATACTCAACCCT+TGG 0.562559 4_1:+81224599 Msa0577060:CDS
TGACCAAAGAAATCATTGCT+TGG 0.564667 4_1:-81225626 None:intergenic
AAGAAATTGAAGGATAATGG+TGG 0.566068 4_1:-81223116 None:intergenic
ACTTCATTTATGCTTCTGAA+AGG 0.566258 4_1:-81226315 None:intergenic
ATATCACAGTTGAATAGTCA+GGG 0.567767 4_1:+81225260 Msa0577060:CDS
GAGACTTGGTCTTGAAGACA+TGG 0.568180 4_1:-81223367 None:intergenic
GTATGGATGAAAGAAATTGA+AGG 0.568953 4_1:-81223126 None:intergenic
ATTGTTTAAGCTCGCGAGGA+GGG 0.569291 4_1:+81225306 Msa0577060:CDS
TCACATGTGTAGTCATCGGT+GGG 0.576365 4_1:-81224542 None:intergenic
TATAATGTTACTCTCAACAT+AGG 0.586798 4_1:+81224039 Msa0577060:CDS
GCAGCATGGTTCATGAGACT+TGG 0.589743 4_1:-81223381 None:intergenic
CATTGTTTAAGCTCGCGAGG+AGG 0.594028 4_1:+81225305 Msa0577060:CDS
GAGAAGCAGTTGATTGGATG+GGG 0.594642 4_1:+81226921 Msa0577060:CDS
GGCTTGGTGCGAAATGTGAT+GGG 0.595102 4_1:+81225281 Msa0577060:CDS
CATATTATAGAGCAGGGTGA+TGG 0.595503 4_1:+81227061 Msa0577060:three_prime_UTR
ATTCAACTGTGATATCAAGC+TGG 0.597014 4_1:-81225252 None:intergenic
TCCATTACTATAGATCATAG+AGG 0.597908 4_1:+81223196 Msa0577060:five_prime_UTR
TTAATGCATATTATAGAGCA+GGG 0.602995 4_1:+81227055 Msa0577060:three_prime_UTR
ACTTGGACTTGGAAGGGGAA+AGG 0.604303 4_1:+81225229 Msa0577060:CDS
TTGGGGTAAGCTATTATGCA+GGG 0.608982 4_1:+81227160 Msa0577060:three_prime_UTR
CTAACACTTAGTTTCACCAA+TGG 0.609380 4_1:+81226357 Msa0577060:CDS
ACTTTACCTATGTGCTGGCA+TGG 0.609706 4_1:+81226285 Msa0577060:CDS
TTGTTTAAGCTCGCGAGGAG+GGG 0.611048 4_1:+81225307 Msa0577060:CDS
ATCATTTAGAAGTACACCAA+GGG 0.613371 4_1:-81224615 None:intergenic
GGACATTGTTTAAGCTCGCG+AGG 0.614419 4_1:+81225302 Msa0577060:CDS
AATATTTAAACTTCCAACCC+CGG 0.615370 4_1:-81225556 None:intergenic
TTAAAGTTAGAATGGCACTT+GGG 0.620505 4_1:+81224672 Msa0577060:CDS
ATTATAAGATAAGCTTCAGG+AGG 0.624826 4_1:-81226408 None:intergenic
ATCTTTGCAAGATTCATGTG+TGG 0.624858 4_1:+81222935 Msa0577060:five_prime_UTR
GAAATTGAAGGATAATGGTG+GGG 0.633191 4_1:-81223114 None:intergenic
TCCTTGTACCCAATGTTCTG+AGG 0.637225 4_1:+81224131 Msa0577060:CDS
ATCCATGCTAGACAAAGTGA+TGG 0.638414 4_1:+81226887 Msa0577060:CDS
ACACTGGTAGCGAACTCACA+TGG 0.642611 4_1:+81224094 Msa0577060:CDS
GGTGAACTGAACCTAATTGG+AGG 0.647086 4_1:+81226591 Msa0577060:CDS
CAAGTATTCCATCTAAAGGG+TGG 0.648915 4_1:-81225211 None:intergenic
GGGTACAAGGAGCATCACAT+TGG 0.654731 4_1:-81224119 None:intergenic
GTTTGTCTAGGCATTCTGAA+CGG 0.654989 4_1:+81226552 Msa0577060:CDS
CATCATTTAGAAGTACACCA+AGG 0.656080 4_1:-81224616 None:intergenic
CAATTGAGGAACCAGCAGCA+TGG 0.660713 4_1:-81223395 None:intergenic
AATTTGACGGCATTAGCACG+AGG 0.660806 4_1:+81227242 Msa0577060:three_prime_UTR
GCATTGTGTAGAGTATATTG+GGG 0.685659 4_1:+81227143 Msa0577060:three_prime_UTR
TCAACATAGGCCAACCACCA+AGG 0.689202 4_1:+81224052 Msa0577060:CDS
TTATAAGATAAGCTTCAGGA+GGG 0.705622 4_1:-81226407 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAATAATAATAATAAAT+GGG - chr4_1:81222856-81222875 None:intergenic 0.0%
!! ATAATAATAATAATAATAAA+TGG - chr4_1:81222857-81222876 None:intergenic 0.0%
!!! TTTATTTTTACTATAATGTA+TGG - chr4_1:81223146-81223165 None:intergenic 10.0%
!!! AATAATGTTTTTCTTTTTTA+AGG + chr4_1:81223515-81223534 Msa0577060:intron 10.0%
!! ATTAAAATTCAATTCATAAA+AGG - chr4_1:81223590-81223609 None:intergenic 10.0%
!!! TGAATTGAATTTTAATTTTT+TGG + chr4_1:81223594-81223613 Msa0577060:intron 10.0%
!!! AAATTGATATTCATTTTTAT+TGG + chr4_1:81223852-81223871 Msa0577060:intron 10.0%
!!! ATTTATATTATTTTCTAGAA+TGG - chr4_1:81225951-81225970 None:intergenic 10.0%
!!! ATTAATAATTTCTAGTATAA+AGG - chr4_1:81226169-81226188 None:intergenic 10.0%
!! TTATACTAGAAATTATTAAT+TGG + chr4_1:81226169-81226188 Msa0577060:intron 10.0%
!!! TTTAAATTATTGATTCTTGT+TGG - chr4_1:81223081-81223100 None:intergenic 15.0%
!! ATATTATATGCAATTTATGT+AGG - chr4_1:81224410-81224429 None:intergenic 15.0%
!! ATGATTTATATAAGTGAATT+AGG + chr4_1:81224837-81224856 Msa0577060:intron 15.0%
!! ATTAACATAAGATATAATCA+AGG + chr4_1:81225884-81225903 Msa0577060:intron 15.0%
!!! AATATTTTGTGATTTCAAAA+TGG + chr4_1:81225976-81225995 Msa0577060:intron 15.0%
!! TAAGTATTTAATGATGAAAT+TGG - chr4_1:81226105-81226124 None:intergenic 15.0%
!! ATGATATTTCATTTCTTTCT+AGG + chr4_1:81223454-81223473 Msa0577060:intron 20.0%
!!! TTTCTTGTTTATCAATTCTT+AGG + chr4_1:81223703-81223722 Msa0577060:intron 20.0%
!!! GGTTTTCTTTTATTTAATTG+TGG - chr4_1:81223808-81223827 None:intergenic 20.0%
!!! ACAGACAAATGTATTTTTTT+GGG + chr4_1:81223875-81223894 Msa0577060:intron 20.0%
!! TGAAATTTATCAATTTCATG+AGG - chr4_1:81224005-81224024 None:intergenic 20.0%
!! TTCACTTATATAAATCATTC+TGG - chr4_1:81224835-81224854 None:intergenic 20.0%
!! AATAGATATGACATAAATTG+TGG - chr4_1:81225103-81225122 None:intergenic 20.0%
!! TTAAATATTATCTTTGACAC+TGG + chr4_1:81225570-81225589 Msa0577060:CDS 20.0%
!!! GTATTTTTAGTTAACTAGTT+TGG + chr4_1:81225818-81225837 Msa0577060:intron 20.0%
!! GCATTTAGAAAATTAACAAA+AGG - chr4_1:81226212-81226231 None:intergenic 20.0%
!! TTGTTAACTGTTAGAATAAA+TGG + chr4_1:81226466-81226485 Msa0577060:intron 20.0%
!! ATATTATGTTATGTAAGTGT+TGG + chr4_1:81226843-81226862 Msa0577060:intron 20.0%
!!! TCTTTAAAAGTACCTAATTA+AGG + chr4_1:81226993-81227012 Msa0577060:three_prime_UTR 20.0%
!!! GTTTTATTACTTTATCTTCA+TGG + chr4_1:81227093-81227112 Msa0577060:three_prime_UTR 20.0%
! TGAATCATTAGATTATTTCC+TGG - chr4_1:81222786-81222805 None:intergenic 25.0%
! AAGTTGAAAAGTTGTAGAAA+AGG - chr4_1:81222891-81222910 None:intergenic 25.0%
! ATAAACTCAACATTAGAAAG+TGG - chr4_1:81223018-81223037 None:intergenic 25.0%
! TGAAAGAAATTGAAGGATAA+TGG - chr4_1:81223122-81223141 None:intergenic 25.0%
! AATTTCAAAGCAACAGAAAA+GGG - chr4_1:81223729-81223748 None:intergenic 25.0%
! AAATTTCAAAGCAACAGAAA+AGG - chr4_1:81223730-81223749 None:intergenic 25.0%
!!! GACAGACAAATGTATTTTTT+TGG + chr4_1:81223874-81223893 Msa0577060:intron 25.0%
!!! TGTATTTTTTTGGGACATTT+CGG + chr4_1:81223884-81223903 Msa0577060:intron 25.0%
!! TGATAAATTTCAACTTTTGC+AGG + chr4_1:81224013-81224032 Msa0577060:intron 25.0%
! TATAATGTTACTCTCAACAT+AGG + chr4_1:81224039-81224058 Msa0577060:CDS 25.0%
! TAATATTACCTCAGAACATT+GGG - chr4_1:81224142-81224161 None:intergenic 25.0%
!!! ATGTGCTGATTATAATTGAT+TGG + chr4_1:81224335-81224354 Msa0577060:intron 25.0%
! CAGCAAAAAAAAAATTACTC+TGG + chr4_1:81224365-81224384 Msa0577060:intron 25.0%
! CAAAAAAAAAATTACTCTGG+TGG + chr4_1:81224368-81224387 Msa0577060:intron 25.0%
!! TTTCATAGTCACATTGATTA+GGG - chr4_1:81224571-81224590 None:intergenic 25.0%
!! ATTTCATAGTCACATTGATT+AGG - chr4_1:81224572-81224591 None:intergenic 25.0%
!! TGATTACATTACTTCTAAGA+GGG - chr4_1:81225676-81225695 None:intergenic 25.0%
! TTGATTACATTACTTCTAAG+AGG - chr4_1:81225677-81225696 None:intergenic 25.0%
! GTAATGTAATCAAGTAGTTA+TGG + chr4_1:81225684-81225703 Msa0577060:intron 25.0%
! TCAAGTAGTTATGGTTATTA+TGG + chr4_1:81225693-81225712 Msa0577060:intron 25.0%
! ATTATGTGTATTACACAACA+GGG - chr4_1:81226018-81226037 None:intergenic 25.0%
! AATTATGTGTATTACACAAC+AGG - chr4_1:81226019-81226038 None:intergenic 25.0%
! ACTAGAAATTATTAATTGGC+AGG + chr4_1:81226173-81226192 Msa0577060:intron 25.0%
! GATATTATAAGATAAGCTTC+AGG - chr4_1:81226414-81226433 None:intergenic 25.0%
!! TGTTATTTGTTGCAAGAATA+TGG + chr4_1:81226524-81226543 Msa0577060:intron 25.0%
!! GTTATTTGTTGCAAGAATAT+GGG + chr4_1:81226525-81226544 Msa0577060:intron 25.0%
! ATTAATGCATATTATAGAGC+AGG + chr4_1:81227054-81227073 Msa0577060:three_prime_UTR 25.0%
! TTAATGCATATTATAGAGCA+GGG + chr4_1:81227055-81227074 Msa0577060:three_prime_UTR 25.0%
! TTATCCTTTTCTTACTCACA+AGG - chr4_1:81222966-81222985 None:intergenic 30.0%
TGTACTAGTTCATGAAGAAA+AGG - chr4_1:81223047-81223066 None:intergenic 30.0%
! AGAAATTGAAGGATAATGGT+GGG - chr4_1:81223118-81223137 None:intergenic 30.0%
! AAGAAATTGAAGGATAATGG+TGG - chr4_1:81223119-81223138 None:intergenic 30.0%
GTATGGATGAAAGAAATTGA+AGG - chr4_1:81223129-81223148 None:intergenic 30.0%
TCCATTACTATAGATCATAG+AGG + chr4_1:81223196-81223215 Msa0577060:five_prime_UTR 30.0%
TCCTCTATGATCTATAGTAA+TGG - chr4_1:81223200-81223219 None:intergenic 30.0%
AACAAGAAGAAGATGAAGAT+AGG + chr4_1:81223222-81223241 Msa0577060:exon 30.0%
! TTTGGTAATAAACGCAAAAG+TGG + chr4_1:81223318-81223337 Msa0577060:CDS 30.0%
ATAAATAGGGATCACAATTG+AGG - chr4_1:81223412-81223431 None:intergenic 30.0%
TCAATTGTGATCCCTATTTA+TGG + chr4_1:81223411-81223430 Msa0577060:CDS 30.0%
GATAAACGTTGCCATAAATA+GGG - chr4_1:81223425-81223444 None:intergenic 30.0%
AATGAAATATCATACCCAAC+AGG - chr4_1:81223447-81223466 None:intergenic 30.0%
AAAACCTCTTCTTACATTGA+TGG + chr4_1:81223822-81223841 Msa0577060:intron 30.0%
AAACCTCTTCTTACATTGAT+GGG + chr4_1:81223823-81223842 Msa0577060:intron 30.0%
AGTTTAAGGGGTTAAAAGAT+CGG - chr4_1:81223961-81223980 None:intergenic 30.0%
GTGTCAACATCAAGAAAATA+GGG - chr4_1:81224078-81224097 None:intergenic 30.0%
AGTGTCAACATCAAGAAAAT+AGG - chr4_1:81224079-81224098 None:intergenic 30.0%
! TATTTTCTTGATGTTGACAC+TGG + chr4_1:81224078-81224097 Msa0577060:CDS 30.0%
GTAATATTACCTCAGAACAT+TGG - chr4_1:81224143-81224162 None:intergenic 30.0%
! ATCATTTAGAAGTACACCAA+GGG - chr4_1:81224618-81224637 None:intergenic 30.0%
AGTCCAACTTAAAGTTAGAA+TGG + chr4_1:81224664-81224683 Msa0577060:CDS 30.0%
TTAAAGTTAGAATGGCACTT+GGG + chr4_1:81224672-81224691 Msa0577060:CDS 30.0%
ATATTTCAAGAGAACATGCA+AGG - chr4_1:81224710-81224729 None:intergenic 30.0%
TTTGTGGAATGGTAAAACTT+AGG - chr4_1:81224997-81225016 None:intergenic 30.0%
TCAAACATGTCATTGTTGAA+AGG - chr4_1:81225066-81225085 None:intergenic 30.0%
TTGATTCCAATATACAGATG+TGG + chr4_1:81225158-81225177 Msa0577060:intron 30.0%
TCATATCCACATCTGTATAT+TGG - chr4_1:81225167-81225186 None:intergenic 30.0%
AAGTTGAAGGTGAAAATATC+TGG - chr4_1:81225190-81225209 None:intergenic 30.0%
ATATCACAGTTGAATAGTCA+GGG + chr4_1:81225260-81225279 Msa0577060:CDS 30.0%
AACGAATCGATTGATGAAAT+TGG - chr4_1:81225383-81225402 None:intergenic 30.0%
AGTCACTAGTATAATACATG+TGG - chr4_1:81225432-81225451 None:intergenic 30.0%
!! TCTTTTATGTTGTTTGTGTC+AGG + chr4_1:81225481-81225500 Msa0577060:intron 30.0%
!! CTTTTATGTTGTTTGTGTCA+GGG + chr4_1:81225482-81225501 Msa0577060:intron 30.0%
!! TTTTATGTTGTTTGTGTCAG+GGG + chr4_1:81225483-81225502 Msa0577060:intron 30.0%
AATATTTAAACTTCCAACCC+CGG - chr4_1:81225559-81225578 None:intergenic 30.0%
TTACCAAGCAATGATTTCTT+TGG + chr4_1:81225623-81225642 Msa0577060:CDS 30.0%
! ATTACATTACTTCTAAGAGG+GGG - chr4_1:81225674-81225693 None:intergenic 30.0%
! GATTACATTACTTCTAAGAG+GGG - chr4_1:81225675-81225694 None:intergenic 30.0%
GTGTTTCTCATTAGTTAGTT+TGG + chr4_1:81225787-81225806 Msa0577060:intron 30.0%
TTTCAGTTTCAGTATATCCA+AGG + chr4_1:81225915-81225934 Msa0577060:intron 30.0%
TTTCTAAATGCAGCTGAATA+AGG + chr4_1:81226221-81226240 Msa0577060:intron 30.0%
ACTTCATTTATGCTTCTGAA+AGG - chr4_1:81226318-81226337 None:intergenic 30.0%
TTATAAGATAAGCTTCAGGA+GGG - chr4_1:81226410-81226429 None:intergenic 30.0%
ATTATAAGATAAGCTTCAGG+AGG - chr4_1:81226411-81226430 None:intergenic 30.0%
AATATGGGAAATGTTTGTCT+AGG + chr4_1:81226540-81226559 Msa0577060:CDS 30.0%
! TTAGCTACATTGTCGTATTT+TGG + chr4_1:81226621-81226640 Msa0577060:intron 30.0%
! AAACTGAAATCTCTGGATTT+TGG - chr4_1:81226969-81226988 None:intergenic 30.0%
AGATCATAAACTGAAATCTC+TGG - chr4_1:81226976-81226995 None:intergenic 30.0%
! AAGCATTGTGTAGAGTATAT+TGG + chr4_1:81227141-81227160 Msa0577060:three_prime_UTR 30.0%
AGCATTGTGTAGAGTATATT+GGG + chr4_1:81227142-81227161 Msa0577060:three_prime_UTR 30.0%
! ATCGTATCACGTTAATTTGA+CGG + chr4_1:81227229-81227248 Msa0577060:three_prime_UTR 30.0%
ATCTTTGCAAGATTCATGTG+TGG + chr4_1:81222935-81222954 Msa0577060:five_prime_UTR 35.0%
CATACCTTGTGAGTAAGAAA+AGG + chr4_1:81222959-81222978 Msa0577060:five_prime_UTR 35.0%
TGATTCTTGTTGGAAAGAGT+TGG - chr4_1:81223071-81223090 None:intergenic 35.0%
! GAAATTGAAGGATAATGGTG+GGG - chr4_1:81223117-81223136 None:intergenic 35.0%
AGAAGAAGATGAAGATAGGT+AGG + chr4_1:81223226-81223245 Msa0577060:exon 35.0%
GAAGAAGATGAAGATAGGTA+GGG + chr4_1:81223227-81223246 Msa0577060:exon 35.0%
! GGAAGAAACTCAATTTTACC+AGG + chr4_1:81223339-81223358 Msa0577060:CDS 35.0%
GGATAAACGTTGCCATAAAT+AGG - chr4_1:81223426-81223445 None:intergenic 35.0%
TGAGTATCCAAATTCATACC+AGG + chr4_1:81223667-81223686 Msa0577060:intron 35.0%
AGAAAGTAATATTCCAGTCC+TGG - chr4_1:81223688-81223707 None:intergenic 35.0%
AACCTCTTCTTACATTGATG+GGG + chr4_1:81223824-81223843 Msa0577060:intron 35.0%
TTAACCCCTTAAACTCAAAC+CGG + chr4_1:81223966-81223985 Msa0577060:intron 35.0%
!! TTCAACCGGTTTGAGTTTAA+GGG - chr4_1:81223974-81223993 None:intergenic 35.0%
ATGTATCTATAGGCAAGGAT+GGG - chr4_1:81224251-81224270 None:intergenic 35.0%
TATGTATCTATAGGCAAGGA+TGG - chr4_1:81224252-81224271 None:intergenic 35.0%
TCAACGCGTTATGTATCTAT+AGG - chr4_1:81224261-81224280 None:intergenic 35.0%
CAGGGTATAAAATCATTGCT+AGG - chr4_1:81224494-81224513 None:intergenic 35.0%
! CATCATTTAGAAGTACACCA+AGG - chr4_1:81224619-81224638 None:intergenic 35.0%
!! AACTTTAAGTTGGACTCCAT+TGG - chr4_1:81224660-81224679 None:intergenic 35.0%
! GTGCCATTCTAACTTTAAGT+TGG - chr4_1:81224670-81224689 None:intergenic 35.0%
CTTAAAGTTAGAATGGCACT+TGG + chr4_1:81224671-81224690 Msa0577060:CDS 35.0%
TCAAATAGCAGATCATGCAT+TGG - chr4_1:81225022-81225041 None:intergenic 35.0%
GACATAAATTGTGGTCATCA+TGG - chr4_1:81225094-81225113 None:intergenic 35.0%
GTCCAAGTATTCCATCTAAA+GGG - chr4_1:81225217-81225236 None:intergenic 35.0%
AGTCCAAGTATTCCATCTAA+AGG - chr4_1:81225218-81225237 None:intergenic 35.0%
TTAGATGGAATACTTGGACT+TGG + chr4_1:81225218-81225237 Msa0577060:CDS 35.0%
ATTCAACTGTGATATCAAGC+TGG - chr4_1:81225255-81225274 None:intergenic 35.0%
GATATCACAGTTGAATAGTC+AGG + chr4_1:81225259-81225278 Msa0577060:CDS 35.0%
ATGATTCTTCTCGAATGAGT+TGG + chr4_1:81225354-81225373 Msa0577060:CDS 35.0%
CTGAGAGTTGAAGTATGTAT+AGG - chr4_1:81225601-81225620 None:intergenic 35.0%
CTATACATACTTCAACTCTC+AGG + chr4_1:81225599-81225618 Msa0577060:CDS 35.0%
TGACCAAAGAAATCATTGCT+TGG - chr4_1:81225629-81225648 None:intergenic 35.0%
TTGGTGAAACTAAGTGTTAG+TGG - chr4_1:81226357-81226376 None:intergenic 35.0%
CTAACACTTAGTTTCACCAA+TGG + chr4_1:81226357-81226376 Msa0577060:CDS 35.0%
CTAAATTCTCACCTCCAATT+AGG - chr4_1:81226605-81226624 None:intergenic 35.0%
!! TGGTGCTACATAGATAGTAA+TGG + chr4_1:81226767-81226786 Msa0577060:intron 35.0%
GTCATATGCATGCCTTAATT+AGG - chr4_1:81227008-81227027 None:intergenic 35.0%
GCATTGTGTAGAGTATATTG+GGG + chr4_1:81227143-81227162 Msa0577060:three_prime_UTR 35.0%
TGGTGAATGAGCAAGTGTTA+AGG - chr4_1:81222817-81222836 None:intergenic 40.0%
AAGATGAAGATAGGTAGGGT+GGG + chr4_1:81223231-81223250 Msa0577060:exon 40.0%
ACTTGTCTTCTTGTTCAGCT+TGG + chr4_1:81223295-81223314 Msa0577060:CDS 40.0%
TCTTCTTGTTCAGCTTGGTT+TGG + chr4_1:81223300-81223319 Msa0577060:CDS 40.0%
TATGGCAACGTTTATCCTGT+TGG + chr4_1:81223429-81223448 Msa0577060:CDS 40.0%
ATGGCAACGTTTATCCTGTT+GGG + chr4_1:81223430-81223449 Msa0577060:CDS 40.0%
ATCCAAATTCATACCAGGAC+TGG + chr4_1:81223672-81223691 Msa0577060:intron 40.0%
TTCCAGTCCTGGTATGAATT+TGG - chr4_1:81223677-81223696 None:intergenic 40.0%
ACCTCTTCTTACATTGATGG+GGG + chr4_1:81223825-81223844 Msa0577060:intron 40.0%
!! TCAACCGGTTTGAGTTTAAG+GGG - chr4_1:81223973-81223992 None:intergenic 40.0%
!! CTTCAACCGGTTTGAGTTTA+AGG - chr4_1:81223975-81223994 None:intergenic 40.0%
ATGAGGTTCTCTACTTCAAC+CGG - chr4_1:81223988-81224007 None:intergenic 40.0%
ACATCAAGAAAATAGGGCCT+TGG - chr4_1:81224072-81224091 None:intergenic 40.0%
GCGTTATGTATCTATAGGCA+AGG - chr4_1:81224256-81224275 None:intergenic 40.0%
GTCTTCACATGTGTAGTCAT+CGG - chr4_1:81224549-81224568 None:intergenic 40.0%
TACCTTCTCAACTTCACCAA+TGG + chr4_1:81224641-81224660 Msa0577060:CDS 40.0%
TGCTATTGTCTCTATGTGTG+AGG - chr4_1:81224969-81224988 None:intergenic 40.0%
! TCTAAAGGGTGGTAAGTTGA+AGG - chr4_1:81225203-81225222 None:intergenic 40.0%
TCAACTTACCACCCTTTAGA+TGG + chr4_1:81225203-81225222 Msa0577060:CDS 40.0%
CAAGTATTCCATCTAAAGGG+TGG - chr4_1:81225214-81225233 None:intergenic 40.0%
CACCCTTTAGATGGAATACT+TGG + chr4_1:81225212-81225231 Msa0577060:CDS 40.0%
ATGGAATACTTGGACTTGGA+AGG + chr4_1:81225222-81225241 Msa0577060:CDS 40.0%
TGGAATACTTGGACTTGGAA+GGG + chr4_1:81225223-81225242 Msa0577060:CDS 40.0%
!!! CTGAACTTGTTTTTGGAGGA+AGG + chr4_1:81225529-81225548 Msa0577060:CDS 40.0%
AGAATGGACACTATAGTCCT+TGG - chr4_1:81225935-81225954 None:intergenic 40.0%
TCATTTGCTCAATCAACTCG+AGG + chr4_1:81226055-81226074 Msa0577060:intron 40.0%
!!! TCATCAGGTGCTGCTTTTAT+TGG - chr4_1:81226261-81226280 None:intergenic 40.0%
ATAGGTAAAGTCTGGTCATC+AGG - chr4_1:81226276-81226295 None:intergenic 40.0%
ACACTTAGTTTCACCAATGG+TGG + chr4_1:81226360-81226379 Msa0577060:CDS 40.0%
AGGTTTAACTCTTCCACCAT+TGG - chr4_1:81226376-81226395 None:intergenic 40.0%
GTTTGTCTAGGCATTCTGAA+CGG + chr4_1:81226552-81226571 Msa0577060:CDS 40.0%
TTGGGTGAACTGAACCTAAT+TGG + chr4_1:81226588-81226607 Msa0577060:CDS 40.0%
! ACACCTTCAGTTTGAAGTGT+TGG + chr4_1:81226747-81226766 Msa0577060:intron 40.0%
ATCCATGCTAGACAAAGTGA+TGG + chr4_1:81226887-81226906 Msa0577060:CDS 40.0%
TACCATCACTTTGTCTAGCA+TGG - chr4_1:81226892-81226911 None:intergenic 40.0%
GGAATACTATTGCAATCTGC+AGG - chr4_1:81226948-81226967 None:intergenic 40.0%
CATATTATAGAGCAGGGTGA+TGG + chr4_1:81227061-81227080 Msa0577060:three_prime_UTR 40.0%
ATTGGGGTAAGCTATTATGC+AGG + chr4_1:81227159-81227178 Msa0577060:three_prime_UTR 40.0%
TTGGGGTAAGCTATTATGCA+GGG + chr4_1:81227160-81227179 Msa0577060:three_prime_UTR 40.0%
GAAGATGAAGATAGGTAGGG+TGG + chr4_1:81223230-81223249 Msa0577060:exon 45.0%
AGATAGGTAGGGTGGGATTA+AGG + chr4_1:81223238-81223257 Msa0577060:CDS 45.0%
GATAGGTAGGGTGGGATTAA+GGG + chr4_1:81223239-81223258 Msa0577060:CDS 45.0%
!! GAGACTTGGTCTTGAAGACA+TGG - chr4_1:81223370-81223389 None:intergenic 45.0%
TCCCCCATCAATGTAAGAAG+AGG - chr4_1:81223829-81223848 None:intergenic 45.0%
CTTCTTACATTGATGGGGGA+AGG + chr4_1:81223829-81223848 Msa0577060:intron 45.0%
TCAAGAAAATAGGGCCTTGG+TGG - chr4_1:81224069-81224088 None:intergenic 45.0%
TCCTTGTACCCAATGTTCTG+AGG + chr4_1:81224131-81224150 Msa0577060:CDS 45.0%
ACCTCAGAACATTGGGTACA+AGG - chr4_1:81224135-81224154 None:intergenic 45.0%
TCATTGCTAGGCTTGTAGAG+TGG - chr4_1:81224482-81224501 None:intergenic 45.0%
GCACAAAGTGGATCTTTGCA+GGG - chr4_1:81224512-81224531 None:intergenic 45.0%
TCACATGTGTAGTCATCGGT+GGG - chr4_1:81224545-81224564 None:intergenic 45.0%
TTCACATGTGTAGTCATCGG+TGG - chr4_1:81224546-81224565 None:intergenic 45.0%
GCTGATCAATACTCAACCCT+TGG + chr4_1:81224599-81224618 Msa0577060:CDS 45.0%
CTCCATTGGTGAAGTTGAGA+AGG - chr4_1:81224646-81224665 None:intergenic 45.0%
ATGCCTAGACGACTACACAA+TGG + chr4_1:81224905-81224924 Msa0577060:intron 45.0%
! ATGCATTGGCGTTTGTGGAA+TGG - chr4_1:81225008-81225027 None:intergenic 45.0%
! AGATCATGCATTGGCGTTTG+TGG - chr4_1:81225013-81225032 None:intergenic 45.0%
GGAATACTTGGACTTGGAAG+GGG + chr4_1:81225224-81225243 Msa0577060:CDS 45.0%
ACAGTTGAATAGTCAGGGCT+TGG + chr4_1:81225265-81225284 Msa0577060:CDS 45.0%
ATTGTTTAAGCTCGCGAGGA+GGG + chr4_1:81225306-81225325 Msa0577060:CDS 45.0%
AGGGGAAAACATTACTCTGC+AGG + chr4_1:81225501-81225520 Msa0577060:intron 45.0%
!! GGACCAGCTGAACTTGTTTT+TGG + chr4_1:81225522-81225541 Msa0577060:CDS 45.0%
CCTCCAAAAACAAGTTCAGC+TGG - chr4_1:81225528-81225547 None:intergenic 45.0%
!!! CCAGCTGAACTTGTTTTTGG+AGG + chr4_1:81225525-81225544 Msa0577060:CDS 45.0%
!! GTTTTTGGAGGAAGGAAGAC+CGG + chr4_1:81225537-81225556 Msa0577060:CDS 45.0%
!! TTTTTGGAGGAAGGAAGACC+GGG + chr4_1:81225538-81225557 Msa0577060:CDS 45.0%
GCTGACGCTTAACACTTTCA+AGG - chr4_1:81225745-81225764 None:intergenic 45.0%
TGATGCGCCAAACTGACTAA+CGG - chr4_1:81225860-81225879 None:intergenic 45.0%
ACCAGACTTTACCTATGTGC+TGG + chr4_1:81226280-81226299 Msa0577060:CDS 45.0%
! ACTTTACCTATGTGCTGGCA+TGG + chr4_1:81226285-81226304 Msa0577060:CDS 45.0%
! CTTTTACCATGCCAGCACAT+AGG - chr4_1:81226294-81226313 None:intergenic 45.0%
GTTCACCCAATCCTACTTCA+GGG - chr4_1:81226578-81226597 None:intergenic 45.0%
AGTTCACCCAATCCTACTTC+AGG - chr4_1:81226579-81226598 None:intergenic 45.0%
GGTGAACTGAACCTAATTGG+AGG + chr4_1:81226591-81226610 Msa0577060:CDS 45.0%
! CTTCAAACTGAAGGTGTGTC+TGG - chr4_1:81226744-81226763 None:intergenic 45.0%
GCACCAACACTTCAAACTGA+AGG - chr4_1:81226753-81226772 None:intergenic 45.0%
GACAACGAGAAGCAGTTGAT+TGG + chr4_1:81226915-81226934 Msa0577060:CDS 45.0%
ACGAGAAGCAGTTGATTGGA+TGG + chr4_1:81226919-81226938 Msa0577060:CDS 45.0%
CGAGAAGCAGTTGATTGGAT+GGG + chr4_1:81226920-81226939 Msa0577060:CDS 45.0%
GAGAAGCAGTTGATTGGATG+GGG + chr4_1:81226921-81226940 Msa0577060:CDS 45.0%
! TGGGAGAGCGTGTGAATGAA+TGG - chr4_1:81222837-81222856 None:intergenic 50.0%
GGTGGGATTAAGGGTGATGA+TGG + chr4_1:81223248-81223267 Msa0577060:CDS 50.0%
CTTGAAGACATGGCTTCACC+TGG - chr4_1:81223360-81223379 None:intergenic 50.0%
GCAGCATGGTTCATGAGACT+TGG - chr4_1:81223384-81223403 None:intergenic 50.0%
AGTCTCATGAACCATGCTGC+TGG + chr4_1:81223384-81223403 Msa0577060:CDS 50.0%
CAATTGAGGAACCAGCAGCA+TGG - chr4_1:81223398-81223417 None:intergenic 50.0%
TCAACATAGGCCAACCACCA+AGG + chr4_1:81224052-81224071 Msa0577060:CDS 50.0%
! GAAAATAGGGCCTTGGTGGT+TGG - chr4_1:81224065-81224084 None:intergenic 50.0%
! ACACTGGTAGCGAACTCACA+TGG + chr4_1:81224094-81224113 Msa0577060:CDS 50.0%
!! GGGTACAAGGAGCATCACAT+TGG - chr4_1:81224122-81224141 None:intergenic 50.0%
CTAGGCTTGTAGAGTGGATG+GGG - chr4_1:81224476-81224495 None:intergenic 50.0%
GCTAGGCTTGTAGAGTGGAT+GGG - chr4_1:81224477-81224496 None:intergenic 50.0%
TGCTAGGCTTGTAGAGTGGA+TGG - chr4_1:81224478-81224497 None:intergenic 50.0%
CGCACAAAGTGGATCTTTGC+AGG - chr4_1:81224513-81224532 None:intergenic 50.0%
CGACCATTGTGTAGTCGTCT+AGG - chr4_1:81224911-81224930 None:intergenic 50.0%
ACTTGGACTTGGAAGGGGAA+AGG + chr4_1:81225229-81225248 Msa0577060:CDS 50.0%
!! GGCTTGGTGCGAAATGTGAT+GGG + chr4_1:81225281-81225300 Msa0577060:CDS 50.0%
GGACATTGTTTAAGCTCGCG+AGG + chr4_1:81225302-81225321 Msa0577060:CDS 50.0%
CATTGTTTAAGCTCGCGAGG+AGG + chr4_1:81225305-81225324 Msa0577060:CDS 50.0%
TTGTTTAAGCTCGCGAGGAG+GGG + chr4_1:81225307-81225326 Msa0577060:CDS 50.0%
GGAGGGGGATACATCTTCTT+TGG + chr4_1:81225323-81225342 Msa0577060:CDS 50.0%
!! TTTTGGAGGAAGGAAGACCG+GGG + chr4_1:81225539-81225558 Msa0577060:CDS 50.0%
GTGCCTTCCGTTAGTCAGTT+TGG + chr4_1:81225850-81225869 Msa0577060:intron 50.0%
GCCAGCACATAGGTAAAGTC+TGG - chr4_1:81226284-81226303 None:intergenic 50.0%
TCAGGAGGGATCTCAAACTG+AGG - chr4_1:81226396-81226415 None:intergenic 50.0%
ATTCTGAACGGCCCTGAAGT+AGG + chr4_1:81226564-81226583 Msa0577060:CDS 50.0%
AACGGCCCTGAAGTAGGATT+GGG + chr4_1:81226570-81226589 Msa0577060:CDS 50.0%
!! GGGCTTGGTGCGAAATGTGA+TGG + chr4_1:81225280-81225299 Msa0577060:CDS 55.0%
TGTTTAAGCTCGCGAGGAGG+GGG + chr4_1:81225308-81225327 Msa0577060:CDS 55.0%
GCGCCAAACTGACTAACGGA+AGG - chr4_1:81225856-81225875 None:intergenic 55.0%
GAACGGCCCTGAAGTAGGAT+TGG + chr4_1:81226569-81226588 Msa0577060:CDS 55.0%
TCTGGTGTGCGAGACATGTC+TGG - chr4_1:81226726-81226745 None:intergenic 55.0%
GGCTGCAAGGACGCACAAAG+TGG - chr4_1:81224524-81224543 None:intergenic 60.0%
!! GTAGTCATCGGTGGGCTGCA+AGG - chr4_1:81224537-81224556 None:intergenic 60.0%
GGAGGAAGGAAGACCGGGGT+TGG + chr4_1:81225543-81225562 Msa0577060:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 81222770 81227261 81222770 ID=Msa0577060;Name=Msa0577060
chr4_1 mRNA 81222770 81227261 81222770 ID=Msa0577060-mRNA-1;Parent=Msa0577060;Name=Msa0577060-mRNA-1;_AED=0.04;_eAED=0.04;_QI=464|0.85|0.87|1|1|1|8|269|412
chr4_1 exon 81222770 81223451 81222770 ID=Msa0577060-mRNA-1:exon:29206;Parent=Msa0577060-mRNA-1
chr4_1 exon 81224035 81224152 81224035 ID=Msa0577060-mRNA-1:exon:29207;Parent=Msa0577060-mRNA-1
chr4_1 exon 81224542 81224693 81224542 ID=Msa0577060-mRNA-1:exon:29208;Parent=Msa0577060-mRNA-1
chr4_1 exon 81225175 81225399 81225175 ID=Msa0577060-mRNA-1:exon:29209;Parent=Msa0577060-mRNA-1
chr4_1 exon 81225503 81225644 81225503 ID=Msa0577060-mRNA-1:exon:29210;Parent=Msa0577060-mRNA-1
chr4_1 exon 81226234 81226434 81226234 ID=Msa0577060-mRNA-1:exon:29211;Parent=Msa0577060-mRNA-1
chr4_1 exon 81226540 81226612 81226540 ID=Msa0577060-mRNA-1:exon:29212;Parent=Msa0577060-mRNA-1
chr4_1 exon 81226883 81227261 81226883 ID=Msa0577060-mRNA-1:exon:29213;Parent=Msa0577060-mRNA-1
chr4_1 five_prime_UTR 81222770 81223233 81222770 ID=Msa0577060-mRNA-1:five_prime_utr;Parent=Msa0577060-mRNA-1
chr4_1 CDS 81223234 81223451 81223234 ID=Msa0577060-mRNA-1:cds;Parent=Msa0577060-mRNA-1
chr4_1 CDS 81224035 81224152 81224035 ID=Msa0577060-mRNA-1:cds;Parent=Msa0577060-mRNA-1
chr4_1 CDS 81224542 81224693 81224542 ID=Msa0577060-mRNA-1:cds;Parent=Msa0577060-mRNA-1
chr4_1 CDS 81225175 81225399 81225175 ID=Msa0577060-mRNA-1:cds;Parent=Msa0577060-mRNA-1
chr4_1 CDS 81225503 81225644 81225503 ID=Msa0577060-mRNA-1:cds;Parent=Msa0577060-mRNA-1
chr4_1 CDS 81226234 81226434 81226234 ID=Msa0577060-mRNA-1:cds;Parent=Msa0577060-mRNA-1
chr4_1 CDS 81226540 81226612 81226540 ID=Msa0577060-mRNA-1:cds;Parent=Msa0577060-mRNA-1
chr4_1 CDS 81226883 81226992 81226883 ID=Msa0577060-mRNA-1:cds;Parent=Msa0577060-mRNA-1
chr4_1 three_prime_UTR 81226993 81227261 81226993 ID=Msa0577060-mRNA-1:three_prime_utr;Parent=Msa0577060-mRNA-1
Gene Sequence

>Msa0577060

ATGAAGATAGGTAGGGTGGGATTAAGGGTGATGATGGTTGCATTGATGATGATGATGTTAAACTTGTCTTCTTGTTCAGCTTGGTTTGGTAATAAACGCAAAAGTGGAAGAAACTCAATTTTACCAGGTGAAGCCATGTCTTCAAGACCAAGTCTCATGAACCATGCTGCTGGTTCCTCAATTGTGATCCCTATTTATGGCAACGTTTATCCTGTTGGGTTCTATAATGTTACTCTCAACATAGGCCAACCACCAAGGCCCTATTTTCTTGATGTTGACACTGGTAGCGAACTCACATGGCTCCAATGTGATGCTCCTTGTACCCAATGTTCTGAGCCCACCGATGACTACACATGTGAAGACCCTAATCAATGTGACTATGAAATTAAGTATGCTGATCAATACTCAACCCTTGGTGTACTTCTAAATGATGTTTACCTTCTCAACTTCACCAATGGAGTCCAACTTAAAGTTAGAATGGCACTTGGATGTGGATATGACCAGATATTTTCACCTTCAACTTACCACCCTTTAGATGGAATACTTGGACTTGGAAGGGGAAAGGCCAGCTTGATATCACAGTTGAATAGTCAGGGCTTGGTGCGAAATGTGATGGGACATTGTTTAAGCTCGCGAGGAGGGGGATACATCTTCTTTGGAAATGCTTATGATTCTTCTCGAATGAGTTGGACTCCAATTTCATCAATCGATTCGGGAAAACATTACTCTGCAGGACCAGCTGAACTTGTTTTTGGAGGAAGGAAGACCGGGGTTGGAAGTTTAAATATTATCTTTGACACTGGAAGTTCCTATACATACTTCAACTCTCAGGCTTACCAAGCAATGATTTCTTTGCTGAATAAGGAATTACATAGAAAGCCAATAAAAGCAGCACCTGATGACCAGACTTTACCTATGTGCTGGCATGGTAAAAGACCTTTCAGAAGCATAAATGAAGTGAAGAAATACTTCAAGCCACTAACACTTAGTTTCACCAATGGTGGAAGAGTTAAACCTCAGTTTGAGATCCCTCCTGAAGCTTATCTTATAATATCAAATATGGGAAATGTTTGTCTAGGCATTCTGAACGGCCCTGAAGTAGGATTGGGTGAACTGAACCTAATTGGAGACATATCCATGCTAGACAAAGTGATGGTATTCGACAACGAGAAGCAGTTGATTGGATGGGGACCTGCAGATTGCAATAGTATTCCAAAATCCAGAGATTTCAGTTTATGA
Protein sequence

>Msa0577060

MKIGRVGLRVMMVALMMMMLNLSSCSAWFGNKRKSGRNSILPGEAMSSRPSLMNHAAGSSIVIPIYGNVYPVGFYNVTLNIGQPPRPYFLDVDTGSELTWLQCDAPCTQCSEPTDDYTCEDPNQCDYEIKYADQYSTLGVLLNDVYLLNFTNGVQLKVRMALGCGYDQIFSPSTYHPLDGILGLGRGKASLISQLNSQGLVRNVMGHCLSSRGGGYIFFGNAYDSSRMSWTPISSIDSGKHYSAGPAELVFGGRKTGVGSLNIIFDTGSSYTYFNSQAYQAMISLLNKELHRKPIKAAPDDQTLPMCWHGKRPFRSINEVKKYFKPLTLSFTNGGRVKPQFEIPPEAYLIISNMGNVCLGILNGPEVGLGELNLIGDISMLDKVMVFDNEKQLIGWGPADCNSIPKSRDFSL*