AlfalfaGEDB | Msa1314140

M. sativa cultivar zhongmu-4 / Msa1314140

KEGG: K05747
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1314140	G7LJ04	64.706	68	22	1	122	189	206	271	4.62e-17	89.0
Msa1314140	G7LJ04	95.238	189	6	1	1	189	1	186	2.85e-124	375

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0176980	Msa1314140	0.809516	1.820542e-50	-8.615850e-47
Msa0207690	Msa1314140	0.800731	1.256161e-48	-8.615850e-47
Msa0287660	Msa1314140	0.800393	1.472284e-48	-8.615850e-47
Msa0553950	Msa1314140	0.851192	1.041670e-60	-8.615850e-47
Msa0608060	Msa1314140	0.810599	1.063764e-50	-8.615850e-47
Msa0627580	Msa1314140	0.807249	5.542177e-50	-8.615850e-47
Msa0690930	Msa1314140	0.809030	2.313762e-50	-8.615850e-47
Msa0719740	Msa1314140	0.801859	7.378854e-49	-8.615850e-47
Msa0802750	Msa1314140	0.808146	3.574415e-50	-8.615850e-47
Msa1026770	Msa1314140	0.811543	6.643267e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1314140	MtrunA17_Chr8g0343091	95.238	189	6	1	1	189	1	186	5.50e-128	375
Msa1314140	MtrunA17_Chr8g0343091	64.706	68	22	1	122	189	206	271	8.89e-21	89.0
Msa1314140	MtrunA17_Chr2g0329841	49.215	191	71	9	1	178	1	178	5.33e-48	162

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1314140	AT1G72790.1	36.649	191	95	6	3	174	2	185	2.39e-26	105

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Find 57 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCCATCGGTGTTTAAT+CGG	0.354028	8_4:-12676419	Msa1314140:CDS
AAATGAAAGAGTATATAGTG+GGG	0.724153	8_4:+12676655	None:intergenic
AACGGTGGCGTTTCGGTGTC+AGG	0.289649	8_4:+12676456	None:intergenic
ACAAGATGTTAAACACTCTT+GGG	0.456069	8_4:-12676610	Msa1314140:CDS
ACACCGAAACGCCACCGTTT+TGG	0.346871	8_4:-12676452	Msa1314140:CDS
ACCTCCGGTCGCCGGAGATC+GGG	0.432859	8_4:+12676375	None:intergenic
ACGGCGGCGATGATCGGTAC+CGG	0.578565	8_4:-12676341	Msa1314140:CDS
ACGGTGGCGTTTCGGTGTCA+GGG	0.565810	8_4:+12676457	None:intergenic
AGAAGATTAGGATTGTTATG+AGG	0.478777	8_4:+12676691	None:intergenic
AGAGGGTGGTGGTTGTTGTG+AGG	0.540215	8_4:+12676721	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAAGATTAGGATTGTTATG+AGG	+	chr8_4:12676394-12676413	None:intergenic	30.0%
ACAAGATGTTAAACACTCTT+GGG	-	chr8_4:12676472-12676491	Msa1314140:CDS	30.0%
TTATAACTATCAAAACGACG+CGG	+	chr8_4:12676581-12676600	None:intergenic	30.0%
TCATCATCACTTTCAAATCT+CGG	+	chr8_4:12676806-12676825	None:intergenic	30.0%
TTAGGATTGTTATGAGGATG+AGG	+	chr8_4:12676388-12676407	None:intergenic	35.0%
CACAAGATGTTAAACACTCT+TGG	-	chr8_4:12676471-12676490	Msa1314140:CDS	35.0%
CACAAGTATCATCGTAAAAC+CGG	+	chr8_4:12676763-12676782	None:intergenic	35.0%
TGTTATGAGGATGAGGAAGA+GGG	+	chr8_4:12676381-12676400	None:intergenic	40.0%
TTGTTATGAGGATGAGGAAG+AGG	+	chr8_4:12676382-12676401	None:intergenic	40.0%
CCACGATGAGTAAGAAGATT+AGG	+	chr8_4:12676406-12676425	None:intergenic	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	12676266	12676838	12676266	ID=Msa1314140;Name=Msa1314140
chr8_4	mRNA	12676266	12676838	12676266	ID=Msa1314140-mRNA-1;Parent=Msa1314140;Name=Msa1314140-mRNA-1;_AED=0.40;_eAED=0.40;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|190
chr8_4	exon	12676266	12676838	12676266	ID=Msa1314140-mRNA-1:exon:4763;Parent=Msa1314140-mRNA-1
chr8_4	CDS	12676266	12676838	12676266	ID=Msa1314140-mRNA-1:cds;Parent=Msa1314140-mRNA-1

Gene Sequence

>Msa1314140

ATGGAACAAGAAGAAGACATATCACCAATGTCACCATTTTGGCTTCCAAAAACCACCACCAACCACCACCGTCTCCGCCGCACATACTCACTCCTCCTCACAACAACCACCACCCTCTTCCTCATCCTCATAACAATCCTAATCTTCTTACTCATCGTGGTCCCCACTATATACTCTTTCATTTCAAACATCTTCAAATTCAAACCACAAGATGTTAAACACTCTTGGGATTACTTAAACCTTATACTAGTTCTCTTCGCTGTCGTTTGTGGTTTTCTTACTAAAAACGACACTAACGAAACGCAAACGACACCGCGTCGTTTTGATAGTTATAATACTCATCGTAGAAGCTTTTCCAACCCTGACACCGAAACGCCACCGTTTTGGTATGGTAATGAAAATCCATCGGTGTTTAATCGGTTGAGAAGTGGTGGATCGTATCCCGATCTCCGGCGACCGGAGGTGGTGGTTGCTGACGGCGGCGATGATCGGTACCGGTTTTACGATGATACTTGTGTTAATTTTCGTTATCGGTATCCGAGATTTGAAAGTGATGATGAATTGCATTACTAG

Protein sequence

>Msa1314140

MEQEEDISPMSPFWLPKTTTNHHRLRRTYSLLLTTTTTLFLILITILIFLLIVVPTIYSFISNIFKFKPQDVKHSWDYLNLILVLFAVVCGFLTKNDTNETQTTPRRFDSYNTHRRSFSNPDTETPPFWYGNENPSVFNRLRSGGSYPDLRRPEVVVADGGDDRYRFYDDTCVNFRYRYPRFESDDELHY*