AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1317970

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1317970 MtrunA17_Chr7g0232921 68.421 76 22 1 1 76 1 74 1.71e-33 116
Msa1317970 MtrunA17_Chr4g0042631 60.870 69 23 1 21 89 33 97 5.89e-26 95.9
Msa1317970 MtrunA17_Chr4g0054601 61.667 60 23 0 72 131 1 60 3.75e-23 87.4
Msa1317970 MtrunA17_Chr6g0473661 45.070 71 39 0 59 129 256 326 1.80e-17 77.4
Msa1317970 MtrunA17_Chr6g0473651 43.056 72 41 0 59 130 225 296 6.17e-17 75.5
Msa1317970 MtrunA17_Chr1g0193281 38.776 98 58 1 28 125 22 117 1.65e-16 72.0
Msa1317970 MtrunA17_Chr1g0207921 35.052 97 63 0 28 124 19 115 7.12e-16 70.5
Msa1317970 MtrunA17_Chr1g0206471 33.333 108 66 2 19 124 14 117 2.31e-15 70.5
Msa1317970 MtrunA17_Chr7g0270401 34.286 105 63 2 28 130 22 122 4.57e-13 63.9
Msa1317970 MtrunA17_Chr6g0473911 43.478 69 25 1 1 55 1 69 4.85e-13 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 54 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTTCAAGTGTTGTCTTAA+TGG 0.101943 8_4:-19773393 Msa1317970:exon
AGCTCAATTGTTGCTGTTAA+AGG 0.143233 8_4:-19773323 Msa1317970:intron
TTTGCTCGTTGTTTGATATA+TGG 0.259333 8_4:-19773532 Msa1317970:five_prime_UTR
TTGCTCGTTGTTTGATATAT+GGG 0.271086 8_4:-19773531 Msa1317970:five_prime_UTR
GTAAATTTCAAAGTAGAAAA+AGG 0.274349 8_4:+19773417 None:intergenic
CCCTAAAATTTGAATTGAAT+TGG 0.279458 8_4:-19773643 Msa1317970:five_prime_UTR
CATATCAACTATTAAGAAAA+AGG 0.285285 8_4:+19770421 None:intergenic
AGTTGATGCTGCTGTTAAAA+AGG 0.315160 8_4:-19773167 Msa1317970:CDS
CAGCTACCTCGATCTCATTC+TGG 0.335624 8_4:+19770553 None:intergenic
TAACCTCAGGACTTTCCATT+TGG 0.366022 8_4:-19773137 Msa1317970:CDS
TAAATAATCATTAGATTATG+AGG 0.369309 8_4:-19771138 Msa1317970:intron
CCACCATGGCGGCCTCTGAT+TGG 0.379949 8_4:+19773578 None:intergenic
TTAACAGCAGCATCAACTTC+AGG 0.389206 8_4:+19773172 None:intergenic
GCGTGCTTTGCAAGAGGGTT+TGG 0.392025 8_4:-19770615 Msa1317970:CDS
TTACTTTCTTCTATTTAAGT+TGG 0.415757 8_4:-19773484 Msa1317970:five_prime_UTR
AAGTGTTGTCTTAATGGCTT+CGG 0.424310 8_4:-19773387 Msa1317970:exon
GTTATAGTACAAGGTAATGC+TGG 0.451080 8_4:-19773196 Msa1317970:intron
TGCAGTAATCATATTCCAAA+TGG 0.451090 8_4:+19773122 None:intergenic
GCCATTAAGACAACACTTGA+AGG 0.466445 8_4:+19773392 None:intergenic
CTGCCTGCGTGCTTTGCAAG+AGG 0.478120 8_4:-19770621 Msa1317970:CDS
AGAAAGTAAATCACATGGAT+TGG 0.483831 8_4:+19773498 None:intergenic
AGAGGGTTTGGTTTGCAAGT+TGG 0.486214 8_4:-19770603 Msa1317970:CDS
AAAAGGATTTGGTTAACCTC+AGG 0.497363 8_4:-19773150 Msa1317970:CDS
TGCCTGCGTGCTTTGCAAGA+GGG 0.503377 8_4:-19770620 Msa1317970:CDS
TCTTCTATTTAAGTTGGAAG+AGG 0.505442 8_4:-19773478 Msa1317970:five_prime_UTR
TAAGAAGGGGTGGCAACTGA+AGG 0.507073 8_4:+19773603 None:intergenic
GGCCTCTGATTGGAATAAGA+AGG 0.511845 8_4:+19773588 None:intergenic
GACAGTTGAATTGAGATCCC+TGG 0.519116 8_4:-19773556 Msa1317970:five_prime_UTR
GCATCACGCAATATCACAAA+AGG 0.522306 8_4:+19770579 None:intergenic
TAGCTGTGGAAAAGAGGAAC+GGG 0.523415 8_4:-19770536 Msa1317970:CDS
GCCTCTGATTGGAATAAGAA+GGG 0.530746 8_4:+19773589 None:intergenic
TGCTCGTTGTTTGATATATG+GGG 0.538969 8_4:-19773530 Msa1317970:five_prime_UTR
TCGAGGTAGCTGTGGAAAAG+AGG 0.543631 8_4:-19770542 Msa1317970:CDS
CCCCTTCTTATTCCAATCAG+AGG 0.550727 8_4:-19773590 Msa1317970:five_prime_UTR
ATCAAACAACGAGCAAAACC+AGG 0.556368 8_4:+19773538 None:intergenic
CTTCTATTTAAGTTGGAAGA+GGG 0.561406 8_4:-19773477 Msa1317970:five_prime_UTR
TTATAGTACAAGGTAATGCT+GGG 0.577463 8_4:-19773195 Msa1317970:intron
ATTCCAATCAGAGGCCGCCA+TGG 0.601566 8_4:-19773581 Msa1317970:five_prime_UTR
GAATGAGATCGAGGTAGCTG+TGG 0.606624 8_4:-19770550 Msa1317970:CDS
CCATTAAGACAACACTTGAA+GGG 0.615112 8_4:+19773393 None:intergenic
TATGTTTGTATAGCGAGGGT+AGG 0.615651 8_4:-19770644 Msa1317970:intron
CAACTGCAGCTAGCCAGGCG+CGG 0.619561 8_4:-19773669 Msa1317970:five_prime_UTR
CTGATTGGAATAAGAAGGGG+TGG 0.626420 8_4:+19773593 None:intergenic
CCAATCAGAGGCCGCCATGG+TGG 0.636349 8_4:-19773578 Msa1317970:five_prime_UTR
AAGAAGGGGTGGCAACTGAA+GGG 0.637209 8_4:+19773604 None:intergenic
TCAAACAACGAGCAAAACCA+GGG 0.640805 8_4:+19773539 None:intergenic
AACCCTCTTGCAAAGCACGC+AGG 0.643073 8_4:+19770618 None:intergenic
GTAGCTGTGGAAAAGAGGAA+CGG 0.653786 8_4:-19770537 Msa1317970:CDS
CTCAATTCAACTGTCCACCA+TGG 0.659375 8_4:+19773564 None:intergenic
CCTCTGATTGGAATAAGAAG+GGG 0.660301 8_4:+19773590 None:intergenic
ATTCCAAATGGAAAGTCCTG+AGG 0.676362 8_4:+19773134 None:intergenic
ATAGAAGAAAGTAAATCACA+TGG 0.677339 8_4:+19773493 None:intergenic
TGCAAACCAGAATGAGATCG+AGG 0.685619 8_4:-19770559 Msa1317970:CDS
AATTCAACTGTCCACCATGG+CGG 0.753831 8_4:+19773567 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTAATAATGATAACAA+AGG - chr8_4:19771488-19771507 Msa1317970:intron 10.0%
!!! ATTTTAATAATGATAACAAA+GGG - chr8_4:19771489-19771508 Msa1317970:intron 10.0%
!! TAATATAAAACTTTACTTAA+AGG + chr8_4:19772694-19772713 None:intergenic 10.0%
!!! AATGAATATCATAAGTTTTA+AGG + chr8_4:19771052-19771071 None:intergenic 15.0%
!! TACTCATAAAATAAACATAA+AGG + chr8_4:19771581-19771600 None:intergenic 15.0%
!! TAAACTTTATTAAGGAAAAA+AGG + chr8_4:19771937-19771956 None:intergenic 15.0%
!! TGTTTCAAAAAAAAAAAGTT+TGG - chr8_4:19771959-19771978 Msa1317970:intron 15.0%
!! TATTACTTAGATTTATGAAA+AGG - chr8_4:19772656-19772675 Msa1317970:intron 15.0%
!! ATTTAACTAAGACAAATATA+TGG - chr8_4:19772716-19772735 Msa1317970:intron 15.0%
!!! TATACTTTTCAAATTAAAGA+TGG + chr8_4:19772806-19772825 None:intergenic 15.0%
!! TAAATAATCATTAGATTATG+AGG - chr8_4:19772954-19772973 Msa1317970:intron 15.0%
!!! CCAATTCAATTCAAATTTTA+GGG + chr8_4:19770452-19770471 None:intergenic 20.0%
!!! ACCAATTCAATTCAAATTTT+AGG + chr8_4:19770453-19770472 None:intergenic 20.0%
!! TTACTTTCTTCTATTTAAGT+TGG - chr8_4:19770608-19770627 Msa1317970:CDS 20.0%
!! GTAAATTTCAAAGTAGAAAA+AGG + chr8_4:19770678-19770697 None:intergenic 20.0%
!!! AGAAATGTTTTATTTTTCTG+AGG - chr8_4:19771119-19771138 Msa1317970:intron 20.0%
!!! CATTTTCCTGTTTTTATTTT+TGG - chr8_4:19771206-19771225 Msa1317970:intron 20.0%
!! CATAAATTATCAAACTGTAA+TGG + chr8_4:19771350-19771369 None:intergenic 20.0%
!! TTATTACAGTTAACTCTAAA+AGG + chr8_4:19771442-19771461 None:intergenic 20.0%
!! GAAACAACTAAACTTTATTA+AGG + chr8_4:19771945-19771964 None:intergenic 20.0%
!! ATTCTCATAATTATGAGATT+TGG - chr8_4:19772233-19772252 Msa1317970:intron 20.0%
!! CTTCAATTATGTACTTTAAA+TGG - chr8_4:19772312-19772331 Msa1317970:intron 20.0%
!! TTGTTAACGTATAAGAAAAA+AGG - chr8_4:19772555-19772574 Msa1317970:intron 20.0%
!!! GATTAATGTTTTTTAGTGAA+TGG - chr8_4:19772882-19772901 Msa1317970:intron 20.0%
!!! ACTGATATGTTAAAAAGTAT+AGG + chr8_4:19772923-19772942 None:intergenic 20.0%
!!! TTTTCTATATGGTTTAACTT+TGG - chr8_4:19773000-19773019 Msa1317970:intron 20.0%
! CCCTAAAATTTGAATTGAAT+TGG - chr8_4:19770449-19770468 Msa1317970:CDS 25.0%
! ATAGAAGAAAGTAAATCACA+TGG + chr8_4:19770602-19770621 None:intergenic 25.0%
!!! TATCATAAGTTTTAAGGTAC+TGG + chr8_4:19771046-19771065 None:intergenic 25.0%
! ATGCATCCAAAAATAAAAAC+AGG + chr8_4:19771215-19771234 None:intergenic 25.0%
! TCATACTGAATAATGACAAA+AGG - chr8_4:19771324-19771343 Msa1317970:intron 25.0%
! GATAACTATGCATACTATTA+TGG - chr8_4:19771385-19771404 Msa1317970:intron 25.0%
!! ATTACTTGCTAAGATAATTG+TGG - chr8_4:19771618-19771637 Msa1317970:intron 25.0%
!! GTTCTTCTATTTGTTAAACA+CGG - chr8_4:19771981-19772000 Msa1317970:intron 25.0%
!!! AGTTGAGAAATGTACTTTTT+TGG - chr8_4:19772009-19772028 Msa1317970:intron 25.0%
!!! AATGTACTTTTTTGGTTTAG+TGG - chr8_4:19772017-19772036 Msa1317970:intron 25.0%
! TACATAAGGCAACTAATAAA+AGG + chr8_4:19772085-19772104 None:intergenic 25.0%
! GGAAAAATCATCATTATCTT+TGG - chr8_4:19772135-19772154 Msa1317970:intron 25.0%
! AGAATTGAAAAAGTTCGTAA+GGG + chr8_4:19772271-19772290 None:intergenic 25.0%
! AAGAATTGAAAAAGTTCGTA+AGG + chr8_4:19772272-19772291 None:intergenic 25.0%
! TTCATGCATATGTTACAATT+AGG - chr8_4:19772348-19772367 Msa1317970:intron 25.0%
!!! CATTTTTTTCCTACTGAAAT+TGG + chr8_4:19772408-19772427 None:intergenic 25.0%
! AGTAAAGTATAGTCATAAGA+AGG + chr8_4:19772527-19772546 None:intergenic 25.0%
! TTGTGAATTGACTAGAAAAA+TGG - chr8_4:19772591-19772610 Msa1317970:intron 25.0%
!!! TGTTGCTTATGTTTTCTATA+TGG - chr8_4:19772989-19773008 Msa1317970:intron 25.0%
!! TATATGGTTTAACTTTGGAT+AGG - chr8_4:19773005-19773024 Msa1317970:intron 25.0%
!!! CACAATTAGTTTTTAGAGTA+TGG + chr8_4:19773190-19773209 None:intergenic 25.0%
!! ATACTCTAAAAATACTTCTG+CGG - chr8_4:19773222-19773241 Msa1317970:intron 25.0%
!!! CTGTTTTGAAGAATTTTTAC+AGG - chr8_4:19773604-19773623 Msa1317970:five_prime_UTR 25.0%
!! AATTTTTACAGGATTTTTGC+TGG - chr8_4:19773615-19773634 Msa1317970:five_prime_UTR 25.0%
TTTGCTCGTTGTTTGATATA+TGG - chr8_4:19770560-19770579 Msa1317970:CDS 30.0%
TTGCTCGTTGTTTGATATAT+GGG - chr8_4:19770561-19770580 Msa1317970:CDS 30.0%
AGAAAGTAAATCACATGGAT+TGG + chr8_4:19770597-19770616 None:intergenic 30.0%
!! TCTTCTATTTAAGTTGGAAG+AGG - chr8_4:19770614-19770633 Msa1317970:CDS 30.0%
!! CTTCTATTTAAGTTGGAAGA+GGG - chr8_4:19770615-19770634 Msa1317970:CDS 30.0%
! AAACTACAGCATGTTTTCAA+TGG - chr8_4:19770732-19770751 Msa1317970:intron 30.0%
GTTATCTGTGTTATAGTACA+AGG - chr8_4:19770887-19770906 Msa1317970:intron 30.0%
TTATAGTACAAGGTAATGCT+GGG - chr8_4:19770897-19770916 Msa1317970:intron 30.0%
TGCAGTAATCATATTCCAAA+TGG + chr8_4:19770973-19770992 None:intergenic 30.0%
CAGTATGAATAGAAGTAACA+TGG + chr8_4:19771312-19771331 None:intergenic 30.0%
TATCTTAGCAAGTAATCCTT+TGG + chr8_4:19771614-19771633 None:intergenic 30.0%
! CTAAGATAATTGTGGAGTTT+TGG - chr8_4:19771626-19771645 Msa1317970:intron 30.0%
!!! ATAATTGTGGAGTTTTGGAT+CGG - chr8_4:19771631-19771650 Msa1317970:intron 30.0%
AAGCGAAAACAACATACAAA+AGG + chr8_4:19771890-19771909 None:intergenic 30.0%
ACTGCATAAGCTTAACTAAA+TGG + chr8_4:19773047-19773066 None:intergenic 30.0%
! CTCATTGAGTTTTCAGAATT+AGG + chr8_4:19773141-19773160 None:intergenic 30.0%
!!! TTTTTAGAGTATGGCAGAAT+TGG + chr8_4:19773181-19773200 None:intergenic 30.0%
! TACTCTAAAAATACTTCTGC+GGG - chr8_4:19773223-19773242 Msa1317970:intron 30.0%
AACATATGTTTGTATAGCGA+GGG - chr8_4:19773444-19773463 Msa1317970:five_prime_UTR 30.0%
!!! AAGCTTATTTTGTTGATGGT+TGG - chr8_4:19773580-19773599 Msa1317970:five_prime_UTR 30.0%
!!! AGCTTATTTTGTTGATGGTT+GGG - chr8_4:19773581-19773600 Msa1317970:five_prime_UTR 30.0%
TGCTCGTTGTTTGATATATG+GGG - chr8_4:19770562-19770581 Msa1317970:CDS 35.0%
CCATTAAGACAACACTTGAA+GGG + chr8_4:19770702-19770721 None:intergenic 35.0%
! AAGTGTTGTCTTAATGGCTT+CGG - chr8_4:19770705-19770724 Msa1317970:intron 35.0%
! AGCTCAATTGTTGCTGTTAA+AGG - chr8_4:19770769-19770788 Msa1317970:intron 35.0%
!! AAAGGTATTGAGATCGAAGT+CGG - chr8_4:19770787-19770806 Msa1317970:intron 35.0%
AAAAATGAAGCTATGCACAC+AGG + chr8_4:19770822-19770841 None:intergenic 35.0%
GTTATAGTACAAGGTAATGC+TGG - chr8_4:19770896-19770915 Msa1317970:intron 35.0%
! AGTTGATGCTGCTGTTAAAA+AGG - chr8_4:19770925-19770944 Msa1317970:intron 35.0%
TGCTGCTGTTAAAAAGGATT+TGG - chr8_4:19770931-19770950 Msa1317970:intron 35.0%
! AAAAGGATTTGGTTAACCTC+AGG - chr8_4:19770942-19770961 Msa1317970:intron 35.0%
TTGTCCTTCTAATTATGCTC+AGG - chr8_4:19771168-19771187 Msa1317970:intron 35.0%
ATTGAGCATGGTTGCATATA+GGG - chr8_4:19771241-19771260 Msa1317970:intron 35.0%
TATGGTGTTTACATAGAGTC+AGG - chr8_4:19771552-19771571 Msa1317970:intron 35.0%
TGAGTAGAGTTGAAATCCAA+AGG - chr8_4:19771595-19771614 Msa1317970:intron 35.0%
!!! TAATTGTGGAGTTTTGGATC+GGG - chr8_4:19771632-19771651 Msa1317970:intron 35.0%
!! TACTTTTGGTGATTGTCTTG+AGG - chr8_4:19771844-19771863 Msa1317970:intron 35.0%
! AAACAACATACAAAAGGCAC+CGG + chr8_4:19771884-19771903 None:intergenic 35.0%
CTGTTACATCCAATTTCAGT+AGG - chr8_4:19772396-19772415 Msa1317970:intron 35.0%
TTAGTGAATGGCAAGTTTCT+TGG - chr8_4:19772894-19772913 Msa1317970:intron 35.0%
TTTCAGAATTAGGTCCAAAG+TGG + chr8_4:19773131-19773150 None:intergenic 35.0%
CGCTATACAAACATATGTTC+AGG + chr8_4:19773443-19773462 None:intergenic 35.0%
GAACATATGTTTGTATAGCG+AGG - chr8_4:19773443-19773462 Msa1317970:five_prime_UTR 35.0%
!!! GGGAAAGCTTATTTTGTTGA+TGG - chr8_4:19773576-19773595 Msa1317970:five_prime_UTR 35.0%
CCTCTGATTGGAATAAGAAG+GGG + chr8_4:19770505-19770524 None:intergenic 40.0%
GCCTCTGATTGGAATAAGAA+GGG + chr8_4:19770506-19770525 None:intergenic 40.0%
TCAAACAACGAGCAAAACCA+GGG + chr8_4:19770556-19770575 None:intergenic 40.0%
ATCAAACAACGAGCAAAACC+AGG + chr8_4:19770557-19770576 None:intergenic 40.0%
! CCCTTCAAGTGTTGTCTTAA+TGG - chr8_4:19770699-19770718 Msa1317970:intron 40.0%
GCCATTAAGACAACACTTGA+AGG + chr8_4:19770703-19770722 None:intergenic 40.0%
TTAACAGCAGCATCAACTTC+AGG + chr8_4:19770923-19770942 None:intergenic 40.0%
TAACCTCAGGACTTTCCATT+TGG - chr8_4:19770955-19770974 Msa1317970:intron 40.0%
ATTCCAAATGGAAAGTCCTG+AGG + chr8_4:19770961-19770980 None:intergenic 40.0%
! CGAATATGACAGAAAGGTAC+TGG + chr8_4:19771022-19771041 None:intergenic 40.0%
ACTGGTCGAATATGACAGAA+AGG + chr8_4:19771028-19771047 None:intergenic 40.0%
CTAGCCTGAGCATAATTAGA+AGG + chr8_4:19771175-19771194 None:intergenic 40.0%
CTTCTAATTATGCTCAGGCT+AGG - chr8_4:19771173-19771192 Msa1317970:intron 40.0%
! ATGCATTGATGCATTGAGCA+TGG - chr8_4:19771229-19771248 Msa1317970:intron 40.0%
CATTGAGCATGGTTGCATAT+AGG - chr8_4:19771240-19771259 Msa1317970:intron 40.0%
TTAAGGCGATGTTGTGGATA+TGG - chr8_4:19771534-19771553 Msa1317970:intron 40.0%
ACAGATGCTGTCACAGAAAA+GGG + chr8_4:19771682-19771701 None:intergenic 40.0%
AACAGATGCTGTCACAGAAA+AGG + chr8_4:19771683-19771702 None:intergenic 40.0%
! TTTTTCACGAACCTCTCTGT+TGG - chr8_4:19771707-19771726 Msa1317970:intron 40.0%
! TTGGCAGCAGAAAGTACATA+AGG + chr8_4:19772099-19772118 None:intergenic 40.0%
TCCTTAATACCCAACCAACT+TGG + chr8_4:19772118-19772137 None:intergenic 40.0%
TGAGAATTGAACTCAGATGC+AGG + chr8_4:19772220-19772239 None:intergenic 40.0%
AATTGGATGTAACAGCTGCA+AGG + chr8_4:19772391-19772410 None:intergenic 40.0%
AAAATACTGCTAGCTGTGTG+TGG + chr8_4:19772620-19772639 None:intergenic 40.0%
CTTAACTAAATGGCAGTGTG+TGG + chr8_4:19773037-19773056 None:intergenic 40.0%
!! AGTGGTCAATATGGTCTTGT+AGG + chr8_4:19773113-19773132 None:intergenic 40.0%
CAAGACCATATTGACCACTT+TGG - chr8_4:19773114-19773133 Msa1317970:intron 40.0%
TAGGTCCAAAGTGGTCAATA+TGG + chr8_4:19773122-19773141 None:intergenic 40.0%
TATGTTTGTATAGCGAGGGT+AGG - chr8_4:19773448-19773467 Msa1317970:five_prime_UTR 40.0%
GCATCACGCAATATCACAAA+AGG + chr8_4:19773516-19773535 None:intergenic 40.0%
!!! ACAGGATTTTTGCTGGTTCT+TGG - chr8_4:19773622-19773641 Msa1317970:five_prime_UTR 40.0%
CTGATTGGAATAAGAAGGGG+TGG + chr8_4:19770502-19770521 None:intergenic 45.0%
CCCCTTCTTATTCCAATCAG+AGG - chr8_4:19770502-19770521 Msa1317970:CDS 45.0%
GGCCTCTGATTGGAATAAGA+AGG + chr8_4:19770507-19770526 None:intergenic 45.0%
AATTCAACTGTCCACCATGG+CGG + chr8_4:19770528-19770547 None:intergenic 45.0%
CTCAATTCAACTGTCCACCA+TGG + chr8_4:19770531-19770550 None:intergenic 45.0%
GACAGTTGAATTGAGATCCC+TGG - chr8_4:19770536-19770555 Msa1317970:CDS 45.0%
TATGCTCAGGCTAGGTAAGT+TGG - chr8_4:19771181-19771200 Msa1317970:intron 45.0%
! TAACAAAGGGCATGTCGAGA+TGG - chr8_4:19771502-19771521 Msa1317970:intron 45.0%
AGGAGATTAAGGCGATGTTG+TGG - chr8_4:19771528-19771547 Msa1317970:intron 45.0%
ACAGGAAAACACCAACAGAG+AGG + chr8_4:19771721-19771740 None:intergenic 45.0%
AACAGGAGCGAGAAAAACAC+AGG + chr8_4:19771739-19771758 None:intergenic 45.0%
CTGAGGGAAAACGGAAAAAC+AGG + chr8_4:19771756-19771775 None:intergenic 45.0%
! TAGGGTGTGTGTGTATTGAC+AGG - chr8_4:19771814-19771833 Msa1317970:intron 45.0%
! AGGGTGTGTGTGTATTGACA+GGG - chr8_4:19771815-19771834 Msa1317970:intron 45.0%
! TGACAGGGGGCTTATACTTT+TGG - chr8_4:19771830-19771849 Msa1317970:intron 45.0%
TGTACTTTCTGCTGCCAAGT+TGG - chr8_4:19772101-19772120 Msa1317970:intron 45.0%
!! TTTCTGCTGCCAAGTTGGTT+GGG - chr8_4:19772106-19772125 Msa1317970:intron 45.0%
!! GCCAAGTTGGTTGGGTATTA+AGG - chr8_4:19772114-19772133 Msa1317970:intron 45.0%
CAGTCAGTTACTTGACGATG+AGG - chr8_4:19773063-19773082 Msa1317970:intron 45.0%
!! AGAGGGTTTGGTTTGCAAGT+TGG - chr8_4:19773489-19773508 Msa1317970:five_prime_UTR 45.0%
TGCAAACCAGAATGAGATCG+AGG - chr8_4:19773533-19773552 Msa1317970:five_prime_UTR 45.0%
GTAGCTGTGGAAAAGAGGAA+CGG - chr8_4:19773555-19773574 Msa1317970:five_prime_UTR 45.0%
TAGCTGTGGAAAAGAGGAAC+GGG - chr8_4:19773556-19773575 Msa1317970:five_prime_UTR 45.0%
!!! NNNNNNNNTTCTAAAATTAT+AGG - chr8_4:19773358-19773377 Msa1317970:CDS 5.0%
!!! AATTTTAGGGTTTCCGCGCC+TGG + chr8_4:19770439-19770458 None:intergenic 50.0%
AAGAAGGGGTGGCAACTGAA+GGG + chr8_4:19770491-19770510 None:intergenic 50.0%
TAAGAAGGGGTGGCAACTGA+AGG + chr8_4:19770492-19770511 None:intergenic 50.0%
GAGCATGGTTGCATATAGGG+TGG - chr8_4:19771244-19771263 Msa1317970:intron 50.0%
!! CGAGATGGTGGAGGAGATTA+AGG - chr8_4:19771517-19771536 Msa1317970:intron 50.0%
!!! GTGGAGTTTTGGATCGGGAT+CGG - chr8_4:19771637-19771656 Msa1317970:intron 50.0%
! GGGTGTGTGTGTATTGACAG+GGG - chr8_4:19771816-19771835 Msa1317970:intron 50.0%
! GGTGTGTGTGTATTGACAGG+GGG - chr8_4:19771817-19771836 Msa1317970:intron 50.0%
! TGAGGTTAGCCGCGTATATC+CGG - chr8_4:19771862-19771881 Msa1317970:intron 50.0%
!! CAAAAGGCACCGGATATACG+CGG + chr8_4:19771874-19771893 None:intergenic 50.0%
!! CTTTCTGCTGCCAAGTTGGT+TGG - chr8_4:19772105-19772124 Msa1317970:intron 50.0%
CAGCTACCTCGATCTCATTC+TGG + chr8_4:19773542-19773561 None:intergenic 50.0%
GAATGAGATCGAGGTAGCTG+TGG - chr8_4:19773542-19773561 Msa1317970:five_prime_UTR 50.0%
TCGAGGTAGCTGTGGAAAAG+AGG - chr8_4:19773550-19773569 Msa1317970:five_prime_UTR 50.0%
ATTCCAATCAGAGGCCGCCA+TGG - chr8_4:19770511-19770530 Msa1317970:CDS 55.0%
! CAAAGGGCATGTCGAGATGG+TGG - chr8_4:19771505-19771524 Msa1317970:intron 55.0%
GCAGCACAGCTGAGGGAAAA+CGG + chr8_4:19771765-19771784 None:intergenic 55.0%
! TTTTCCCTCAGCTGTGCTGC+TGG - chr8_4:19771765-19771784 Msa1317970:intron 55.0%
AAGACCAGCAGCACAGCTGA+GGG + chr8_4:19771772-19771791 None:intergenic 55.0%
AAAGACCAGCAGCACAGCTG+AGG + chr8_4:19771773-19771792 None:intergenic 55.0%
!! CAGCTGTGCTGCTGGTCTTT+CGG - chr8_4:19771773-19771792 Msa1317970:intron 55.0%
!! AGCTGTGCTGCTGGTCTTTC+GGG - chr8_4:19771774-19771793 Msa1317970:intron 55.0%
! GTAGGCAGGTGGGATTGGTA+GGG - chr8_4:19771796-19771815 Msa1317970:intron 55.0%
CTTGACAACACGTGCTGCAG+TGG + chr8_4:19772464-19772483 None:intergenic 55.0%
TGCCTGCGTGCTTTGCAAGA+GGG - chr8_4:19773472-19773491 Msa1317970:five_prime_UTR 55.0%
AACCCTCTTGCAAAGCACGC+AGG + chr8_4:19773477-19773496 None:intergenic 55.0%
GCGTGCTTTGCAAGAGGGTT+TGG - chr8_4:19773477-19773496 Msa1317970:five_prime_UTR 55.0%
! AGGGCATGTCGAGATGGTGG+AGG - chr8_4:19771508-19771527 Msa1317970:intron 60.0%
!! GTGCTGCTGGTCTTTCGGGT+AGG - chr8_4:19771778-19771797 Msa1317970:intron 60.0%
!! TGCTGGTCTTTCGGGTAGGC+AGG - chr8_4:19771782-19771801 Msa1317970:intron 60.0%
! TGGTCTTTCGGGTAGGCAGG+TGG - chr8_4:19771785-19771804 Msa1317970:intron 60.0%
! GGTCTTTCGGGTAGGCAGGT+GGG - chr8_4:19771786-19771805 Msa1317970:intron 60.0%
TTCGGGTAGGCAGGTGGGAT+TGG - chr8_4:19771791-19771810 Msa1317970:intron 60.0%
! GGTAGGCAGGTGGGATTGGT+AGG - chr8_4:19771795-19771814 Msa1317970:intron 60.0%
TTGCATTGTGCGTCTCGCGC+AGG - chr8_4:19771908-19771927 Msa1317970:intron 60.0%
CTGCCTGCGTGCTTTGCAAG+AGG - chr8_4:19773471-19773490 Msa1317970:five_prime_UTR 60.0%
CCACCATGGCGGCCTCTGAT+TGG + chr8_4:19770517-19770536 None:intergenic 65.0%
CCAATCAGAGGCCGCCATGG+TGG - chr8_4:19770514-19770533 Msa1317970:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 19770425 19773689 19770425 ID=Msa1317970;Name=Msa1317970
chr8_4 mRNA 19770425 19773689 19770425 ID=Msa1317970-mRNA-1;Parent=Msa1317970;Name=Msa1317970-mRNA-1;_AED=0.60;_eAED=0.60;_QI=293|0.33|0.25|0.5|0.33|0.75|4|0|133
chr8_4 exon 19770425 19770653 19770425 ID=Msa1317970-mRNA-1:exon:7239;Parent=Msa1317970-mRNA-1
chr8_4 exon 19771139 19771146 19771139 ID=Msa1317970-mRNA-1:exon:7238;Parent=Msa1317970-mRNA-1
chr8_4 exon 19773120 19773211 19773120 ID=Msa1317970-mRNA-1:exon:7237;Parent=Msa1317970-mRNA-1
chr8_4 exon 19773324 19773689 19773324 ID=Msa1317970-mRNA-1:exon:7236;Parent=Msa1317970-mRNA-1
chr8_4 five_prime_UTR 19773397 19773689 19773397 ID=Msa1317970-mRNA-1:five_prime_utr;Parent=Msa1317970-mRNA-1
chr8_4 CDS 19773324 19773396 19773324 ID=Msa1317970-mRNA-1:cds;Parent=Msa1317970-mRNA-1
chr8_4 CDS 19773120 19773211 19773120 ID=Msa1317970-mRNA-1:cds;Parent=Msa1317970-mRNA-1
chr8_4 CDS 19771139 19771146 19771139 ID=Msa1317970-mRNA-1:cds;Parent=Msa1317970-mRNA-1
chr8_4 CDS 19770425 19770653 19770425 ID=Msa1317970-mRNA-1:cds;Parent=Msa1317970-mRNA-1
Gene Sequence

>Msa1317970

ATGGCTTCGGTTGAAAACTACAGCATGTTTTCAATGGATCGTTCGTATGCAAGCTCAATTGTTGCTGTTAAAGTACAAGGTAATGCTGGGCCTGAAGTTGATGCTGCTGTTAAAAAGGATTTGGTTAACCTCAGGACTTTCCATTTGGAATATGATTACTGCAGTATTATGAGCGAGGGTAGGCTGCCTGCGTGCTTTGCAAGAGGGTTTGGTTTGCAAGTTGGTCCTTTTGTGATATTGCGTGATGCAAACCAGAATGAGATCGAGGTAGCTGTGGAAAAGAGGAACGGGAAAGCTTATTTTGTTGATGGTTGGGCTGTTTTGAAGAATTTTTACAGGATTTTTGCTGGTTCTTGGATCACTGTCATTTTTGCAAATCGACGCCTTTTTCTTAATAGTTGA
Protein sequence

>Msa1317970

MASVENYSMFSMDRSYASSIVAVKVQGNAGPEVDAAVKKDLVNLRTFHLEYDYCSIMSEGRLPACFARGFGLQVGPFVILRDANQNEIEVAVEKRNGKAYFVDGWAVLKNFYRIFAGSWITVIFANRRLFLNS*