AlfalfaGEDB | Msa1331120

M. sativa cultivar zhongmu-4 / Msa1331120

KEGG: -
GO: -
PFAM: PF10250.12

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1331120	sp\|Q4V398\|RRT1_ARATH	72.746	477	121	3	35	510	31	499	0.0	742

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1331120	A0A072URS3	97.885	520	8	2	1	519	1	518	0.0	1052

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0052870	Msa1331120	0.808172	3.528440e-50	-8.615850e-47
Msa0054880	Msa1331120	0.805534	1.274291e-49	-8.615850e-47
Msa0298500	Msa1331120	0.810668	1.028192e-50	-8.615850e-47
Msa0307570	Msa1331120	0.808681	2.748409e-50	-8.615850e-47
Msa0336390	Msa1331120	0.803290	3.741252e-49	-8.615850e-47
Msa0702590	Msa1331120	0.807054	6.096870e-50	-8.615850e-47
Msa1212890	Msa1331120	0.862539	4.717549e-64	-8.615850e-47
Msa1224840	Msa1331120	0.803915	2.775267e-49	-8.615850e-47
Msa1234710	Msa1331120	0.806987	6.298413e-50	-8.615850e-47
Msa1300250	Msa1331120	0.838258	3.199939e-57	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1331120	MtrunA17_Chr4g0061781	97.885	520	8	2	1	519	1	518	0.0	1052
Msa1331120	MtrunA17_Chr2g0307971	79.961	519	91	4	3	519	12	519	0.0	848
Msa1331120	MtrunA17_Chr6g0455571	77.637	474	101	2	42	515	45	513	0.0	776
Msa1331120	MtrunA17_Chr5g0417871	60.805	472	177	5	42	509	9	476	0.0	598
Msa1331120	MtrunA17_Chr4g0042711	60.510	471	177	4	40	509	26	488	0.0	595
Msa1331120	MtrunA17_Chr4g0070551	48.601	393	193	5	114	505	227	611	5.46e-129	389
Msa1331120	MtrunA17_Chr5g0426591	48.515	404	197	5	114	509	110	510	2.02e-128	384
Msa1331120	MtrunA17_Chr4g0008451	49.109	393	191	5	114	505	231	615	1.09e-126	384
Msa1331120	MtrunA17_Chr5g0436321	46.929	407	204	7	116	518	162	560	3.18e-125	377
Msa1331120	MtrunA17_Chr3g0132371	48.889	405	189	7	115	509	141	537	2.81e-121	367

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1331120	AT5G15740.1	72.746	477	121	3	35	510	31	499	0.0	742
Msa1331120	AT3G02250.1	72.881	472	119	3	40	510	36	499	0.0	728
Msa1331120	AT1G14020.1	59.915	469	174	4	42	509	27	482	0.0	597
Msa1331120	AT2G03280.1	56.992	472	182	7	40	509	28	480	0.0	557
Msa1331120	AT2G03280.2	53.908	499	182	8	40	509	28	507	0.0	543
Msa1331120	AT2G37980.1	50.251	398	188	7	114	509	225	614	1.05e-130	394
Msa1331120	AT5G01100.1	48.635	403	185	7	112	505	213	602	2.14e-126	383
Msa1331120	AT3G54100.1	47.739	398	198	7	114	509	221	610	1.16e-123	376
Msa1331120	AT1G22460.1	45.823	419	205	7	105	509	146	556	7.45e-123	372
Msa1331120	AT5G35570.1	47.570	391	196	6	116	505	252	634	1.49e-122	374

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Find 188 sgRNAs with CRISPR-Local

Find 297 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGTACTAACTCTTGGG+AGG	0.632505	8_4:+50528748	None:intergenic
AAACCAAGTGTACCAGGAGA+TGG	0.627844	8_4:+50526162	None:intergenic
AAACTATAGAACGAGTTAAG+GGG	0.632455	8_4:-50529592	Msa1331120:five_prime_UTR
AAATAGAACGGATGCTCGGC+TGG	0.442470	8_4:-50527585	Msa1331120:CDS
AAATATGGCCAAAGTAGTTG+CGG	0.588468	8_4:-50526780	Msa1331120:CDS
AACAGCTATAGGTGATTTGT+GGG	0.454993	8_4:-50528881	Msa1331120:CDS
AACTACAAGTGGCAAGATTT+GGG	0.370307	8_4:-50529042	Msa1331120:CDS
AACTGTGCAGATATGTGACA+TGG	0.717484	8_4:-50528128	Msa1331120:intron
AACTTGATAAAACATCCTTT+TGG	0.293087	8_4:-50528063	Msa1331120:CDS
AAGACGGACAAGTCTTTCAA+AGG	0.582155	8_4:-50527039	Msa1331120:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACTATAGAACGAGTTAAG+GGG	-	chr8_4:50525776-50525795	Msa1331120:three_prime_UTR	30.0%
TGAATGAATATTATAGCCGA+TGG	+	chr8_4:50525933-50525952	None:intergenic	30.0%
TGTTGAATTTGAGGAAATGA+AGG	+	chr8_4:50526031-50526050	None:intergenic	30.0%
CAATATAAAAAATAACCGCG+CGG	-	chr8_4:50526064-50526083	Msa1331120:three_prime_UTR	30.0%
ACAAATAAAAATTAACCGCG+CGG	+	chr8_4:50526082-50526101	None:intergenic	30.0%
GTAAAAGCAATAACTACAAG+TGG	-	chr8_4:50526315-50526334	Msa1331120:CDS	30.0%
TAAAAACAATGGGTACTTGA+TGG	-	chr8_4:50526832-50526851	Msa1331120:CDS	30.0%
TTATATAACCCCTACATTTC+AGG	+	chr8_4:50526947-50526966	None:intergenic	30.0%
ATTTAGTCCTCAAGATTGTA+GGG	+	chr8_4:50527115-50527134	None:intergenic	30.0%
TATTTAGTCCTCAAGATTGT+AGG	+	chr8_4:50527116-50527135	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	50525761	50529629	50525761	ID=Msa1331120;Name=Msa1331120
chr8_4	mRNA	50525761	50529629	50525761	ID=Msa1331120-mRNA-1;Parent=Msa1331120;Name=Msa1331120-mRNA-1;_AED=0.06;_eAED=0.06;_QI=538\|0.85\|0.87\|1\|1\|1\|8\|461\|519
chr8_4	exon	50525761	50526468	50525761	ID=Msa1331120-mRNA-1:exon:14794;Parent=Msa1331120-mRNA-1
chr8_4	exon	50526770	50526927	50526770	ID=Msa1331120-mRNA-1:exon:14793;Parent=Msa1331120-mRNA-1
chr8_4	exon	50527025	50527229	50527025	ID=Msa1331120-mRNA-1:exon:14792;Parent=Msa1331120-mRNA-1
chr8_4	exon	50527356	50527645	50527356	ID=Msa1331120-mRNA-1:exon:14791;Parent=Msa1331120-mRNA-1
chr8_4	exon	50527765	50527930	50527765	ID=Msa1331120-mRNA-1:exon:14790;Parent=Msa1331120-mRNA-1
chr8_4	exon	50528052	50528140	50528052	ID=Msa1331120-mRNA-1:exon:14789;Parent=Msa1331120-mRNA-1
chr8_4	exon	50528492	50528565	50528492	ID=Msa1331120-mRNA-1:exon:14788;Parent=Msa1331120-mRNA-1
chr8_4	exon	50528761	50529629	50528761	ID=Msa1331120-mRNA-1:exon:14787;Parent=Msa1331120-mRNA-1
chr8_4	five_prime_UTR	50529092	50529629	50529092	ID=Msa1331120-mRNA-1:five_prime_utr;Parent=Msa1331120-mRNA-1
chr8_4	CDS	50528761	50529091	50528761	ID=Msa1331120-mRNA-1:cds;Parent=Msa1331120-mRNA-1
chr8_4	CDS	50528492	50528565	50528492	ID=Msa1331120-mRNA-1:cds;Parent=Msa1331120-mRNA-1
chr8_4	CDS	50528052	50528140	50528052	ID=Msa1331120-mRNA-1:cds;Parent=Msa1331120-mRNA-1
chr8_4	CDS	50527765	50527930	50527765	ID=Msa1331120-mRNA-1:cds;Parent=Msa1331120-mRNA-1
chr8_4	CDS	50527356	50527645	50527356	ID=Msa1331120-mRNA-1:cds;Parent=Msa1331120-mRNA-1
chr8_4	CDS	50527025	50527229	50527025	ID=Msa1331120-mRNA-1:cds;Parent=Msa1331120-mRNA-1
chr8_4	CDS	50526770	50526927	50526770	ID=Msa1331120-mRNA-1:cds;Parent=Msa1331120-mRNA-1
chr8_4	CDS	50526222	50526468	50526222	ID=Msa1331120-mRNA-1:cds;Parent=Msa1331120-mRNA-1
chr8_4	three_prime_UTR	50525761	50526221	50525761	ID=Msa1331120-mRNA-1:three_prime_utr;Parent=Msa1331120-mRNA-1

Gene Sequence

>Msa1331120

ATGTGTAAACCAGAGAGTAAAAGCAATAACTACAAGTGGCAAGATTTGGGTTGGATGGGTTTGAAGGCACAAAAAGAGAGACTAGCTTCGGGTTCGGGTTTAGGTTCGGGTTCAGGAAGAGGTAGAATGAAACTGTGGATGATACGTGCAACTACAACGGTGATATTGTGGACTTGTATAGTTCAATTAACAGCTATAGGTGATTTGTGGGGTCCTCGAGTTCTTAAGGGTTGGCCTTCGTGTTTTACACATGTGGATTCTTCTGCTTCAAATTCTAGAGCGTTGGATAATAATGTTCAGTTTCTGCGTCCACCAGTTCTTCCTCCCAAGAGAGTTTATAAAAACAATGGGTACTTGATGGTTTCGTGCAATGGAGGATTGAATCAAATGAGAGCAGCGGTGGTGATATGTGACATGGTTGCCATTGCCAGATATTTAAATGTCACGCTTATAGTTCCTGAACTTGATAAAACATCCTTTTGGGCTGATCCTAGTGAATTCCAAGACATATTTGATGTGGATCATTTTATCACATCCTTGAGAGATGAGGTGCGGATATTGAAAGAGTTGCCTCCTAGACTCAAGCTGAAAGTGGAACGTGGTTTCCTTTACACTATGCCACCCGTTAGTTGGTCCGACATATCTTACTATAAGAACCAGATTCTACCCTTGATACAAAAGCACAAAGTTGTCCATCTAAATAGAACGGATGCTCGGCTGGCCAATAACGGCCAACCTCCAGAGATTCAGAAGCTGCGATGCAGAGTTAATTTTGGTGCTCTGAGATTCACTTCTCAGATAGAGGAGTTGGGCAGAAAGGTGATCAAGCTTCTAAGGAAAAACGGTCCATTTCTGGTCCTTCATCTTAGGTATGAGATGGACATGTTAGCATTTTCTGGCTGTACTCAAGGTTGTAACAGTGATGAGGTGGAAGAGTTGACAAGAATGAGATATGCCTATCCTTGGTGGAAAGAGAAAATAATTAATTCCGACTTGAAAAGAAGAGATGGTTTATGTCCTCTAACACCCGAGGAGACTGCCCTTACACTAAGGGCTCTTGACATTGATCGAAACATTCAAATCTACATTGCAGCCGGTGAAATATATGGTGGAGAAAGAAGACGGACAAGTCTTTCAAAGGAGTACCCAAAATTGGTCAGGAAGGAAACACTGTTGGAGCCATCCGATCTTCGTTTCTTTCAAAATCATTCATCTCAGATGGCAGCATTGGATTATCTTGTGTCGTTAGAGAGTGATATTTTTGTTCCCACATATGATGGAAATATGGCCAAAGTAGTTGCGGGCCACCGCAGATATCTTGGGTTCAAGAAGACGATTTTACTGGACAGAAAGCTCTTAGTTGATTTAATAGATCAGTACAATAACGGTGCACTGAACTGGGACCAATTTTCTTCAGCTGTGAAGGAATCGCATGCTCATCGCATGGGTAACCCTAGTAAAAGGTTGGTAATTCCTGACAAGCCCAAAGAAGAGGATTATTTCTATTCCAACCCAGAGGAATGTTTAGAACCATCAAATGATATGCTGAGTAGCACGTGA

Protein sequence

>Msa1331120

MCKPESKSNNYKWQDLGWMGLKAQKERLASGSGLGSGSGRGRMKLWMIRATTTVILWTCIVQLTAIGDLWGPRVLKGWPSCFTHVDSSASNSRALDNNVQFLRPPVLPPKRVYKNNGYLMVSCNGGLNQMRAAVVICDMVAIARYLNVTLIVPELDKTSFWADPSEFQDIFDVDHFITSLRDEVRILKELPPRLKLKVERGFLYTMPPVSWSDISYYKNQILPLIQKHKVVHLNRTDARLANNGQPPEIQKLRCRVNFGALRFTSQIEELGRKVIKLLRKNGPFLVLHLRYEMDMLAFSGCTQGCNSDEVEELTRMRYAYPWWKEKIINSDLKRRDGLCPLTPEETALTLRALDIDRNIQIYIAAGEIYGGERRRTSLSKEYPKLVRKETLLEPSDLRFFQNHSSQMAALDYLVSLESDIFVPTYDGNMAKVVAGHRRYLGFKKTILLDRKLLVDLIDQYNNGALNWDQFSSAVKESHAHRMGNPSKRLVIPDKPKEEDYFYSNPEECLEPSNDMLSST*