AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1349100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1349100 MtrunA17_Chr5g0414241 68.852 61 17 1 62 122 91 149 9.47e-23 88.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1349100 AT1G24095.2 52.542 59 26 1 64 122 22 78 1.34e-13 63.5
Msa1349100 AT1G24095.1 52.542 59 26 1 64 122 88 144 3.99e-13 63.5

Find 48 sgRNAs with CRISPR-Local

Find 250 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAATATTCTTTCAACTAAC+TGG 0.262416 tig0010653:-70261 Msa1349100:CDS
TGTATTGGCAGGAGCAAAAT+TGG 0.268687 tig0010653:-69082 Msa1349100:intron
CTTCTTTCTCTTATCCCTCT+TGG 0.335559 tig0010653:-70720 Msa1349100:five_prime_UTR
AATAGCGACGACTCCGATAA+TGG 0.373948 tig0010653:-70238 Msa1349100:CDS
CCGGATTGAAATCGAAAATC+AGG 0.381004 tig0010653:+70849 None:intergenic
ATGTGGTGTCGTCTCCTTCA+AGG 0.387809 tig0010653:-69713 Msa1349100:CDS
CATTGAGTCTCTCTTCTTCA+AGG 0.404900 tig0010653:-70654 Msa1349100:five_prime_UTR
GTCGTAGGACATCCTCTTGT+TGG 0.419317 tig0010653:+68968 None:intergenic
AATAGAAGACTAACCCTTGA+AGG 0.429327 tig0010653:+69699 None:intergenic
GTATTCTCCCCGGGTTCTAC+TGG 0.442067 tig0010653:-68919 Msa1349100:CDS
CCTCTTGGTAACGACTTCTC+TGG 0.444996 tig0010653:-70705 Msa1349100:five_prime_UTR
TTCTCTGGAACCACCACACC+GGG 0.456478 tig0010653:-70690 Msa1349100:five_prime_UTR
GTTTATGACGTCGAGGTGGC+CGG 0.458703 tig0010653:+70830 None:intergenic
ACTTGGTGAAACACCATTAT+CGG 0.468409 tig0010653:+70225 None:intergenic
GACATCCTCTTGTTGGGTAC+CGG 0.470554 tig0010653:+68975 None:intergenic
ACCATTGTCAATCGGAGACT+TGG 0.490959 tig0010653:+70208 None:intergenic
GAGACCTATTTGAGAGCATC+CGG 0.491379 tig0010653:-68994 Msa1349100:CDS
TCGTAGGACATCCTCTTGTT+GGG 0.505389 tig0010653:+68969 None:intergenic
GGTACCGGATGCTCTCAAAT+AGG 0.506052 tig0010653:+68990 None:intergenic
AGGCTTGAATGTATTCTCCC+CGG 0.514935 tig0010653:-68929 Msa1349100:CDS
GGCTTGAATGTATTCTCCCC+GGG 0.546853 tig0010653:-68928 Msa1349100:CDS
TGTGGTGTCGTCTCCTTCAA+GGG 0.550044 tig0010653:-69712 Msa1349100:intron
GAAAATCAGGGCTGAATGTG+AGG 0.553922 tig0010653:+70862 None:intergenic
ATTGAGTCTCTCTTCTTCAA+GGG 0.557749 tig0010653:-70653 Msa1349100:intron
TGAGCCATACCATTGTCAAT+CGG 0.566373 tig0010653:+70200 None:intergenic
CTTCTCTGGAACCACCACAC+CGG 0.571109 tig0010653:-70691 Msa1349100:five_prime_UTR
TCGGAATGACCCCGGTGTGG+TGG 0.573200 tig0010653:+70680 None:intergenic
GTCTCCGATTGACAATGGTA+TGG 0.594789 tig0010653:-70204 Msa1349100:CDS
CAGAGAAGTCGTTACCAAGA+GGG 0.601246 tig0010653:+70706 None:intergenic
ACCAAGTCTCCGATTGACAA+TGG 0.601398 tig0010653:-70209 Msa1349100:CDS
CGGATTGAAATCGAAAATCA+GGG 0.603867 tig0010653:+70850 None:intergenic
ATAGTAGAATTCATATATTG+TGG 0.605035 tig0010653:+69738 None:intergenic
GAATTCTAACCAGTAGAACC+CGG 0.608560 tig0010653:+68910 None:intergenic
TTCAGTTTATGACGTCGAGG+TGG 0.612924 tig0010653:+70826 None:intergenic
AAAATCAGGGCTGAATGTGA+GGG 0.622618 tig0010653:+70863 None:intergenic
AATTCTAACCAGTAGAACCC+GGG 0.623780 tig0010653:+68911 None:intergenic
AGCATCCGGTACCCAACAAG+AGG 0.628194 tig0010653:-68980 Msa1349100:CDS
ATAAGAGAAAGAAGATAACG+AGG 0.637273 tig0010653:+70729 None:intergenic
TAGTTGAAAGAATATTGCAG+AGG 0.649071 tig0010653:+70266 None:intergenic
TATGAATTCTACTATCGATG+TGG 0.654067 tig0010653:-69730 Msa1349100:CDS
CAATGACGTCGGAATGACCC+CGG 0.658724 tig0010653:+70672 None:intergenic
AAGCCGACAAGGATAAAACA+AGG 0.669427 tig0010653:-69053 Msa1349100:CDS
CCAGAGAAGTCGTTACCAAG+AGG 0.671333 tig0010653:+70705 None:intergenic
ATTGGTAATCAAAGCCGACA+AGG 0.673145 tig0010653:-69064 Msa1349100:CDS
GAAGAGAGACTCAATGACGT+CGG 0.675925 tig0010653:+70661 None:intergenic
ATTCTAACCAGTAGAACCCG+GGG 0.714249 tig0010653:+68912 None:intergenic
ACGTCGGAATGACCCCGGTG+TGG 0.729323 tig0010653:+70677 None:intergenic
TCTCTGGAACCACCACACCG+GGG 0.768752 tig0010653:-70689 Msa1349100:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATTAAAAGATAATGA+AGG + tig0010653:70011-70030 None:intergenic 10.0%
!! AAAAAATTAAAAGATAATGA+AGG + tig0010653:70011-70030 None:intergenic 10.0%
!!! TATAATTTGAAACATTCTTT+AGG + tig0010653:70180-70199 None:intergenic 15.0%
!! TATTAGGGTTATTATTTATT+AGG - tig0010653:70221-70240 Msa1349100:CDS 15.0%
!!! TGTTTTTTTCTATGTTATTT+AGG - tig0010653:70269-70288 Msa1349100:CDS 15.0%
!!! GTTTTTTTCTATGTTATTTA+GGG - tig0010653:70270-70289 Msa1349100:CDS 15.0%
!! ATTTAACAAAAAAAAAAGAG+TGG - tig0010653:70333-70352 Msa1349100:intron 15.0%
!! TAAATAAATTGAGCTATTTA+TGG + tig0010653:70477-70496 None:intergenic 15.0%
!!! TATAATTTGAAACATTCTTT+AGG + tig0010653:70180-70199 None:intergenic 15.0%
!! TATTAGGGTTATTATTTATT+AGG - tig0010653:70221-70240 Msa1349100:CDS 15.0%
!!! TGTTTTTTTCTATGTTATTT+AGG - tig0010653:70269-70288 Msa1349100:CDS 15.0%
!!! GTTTTTTTCTATGTTATTTA+GGG - tig0010653:70270-70289 Msa1349100:CDS 15.0%
!! ATTTAACAAAAAAAAAAGAG+TGG - tig0010653:70333-70352 Msa1349100:intron 15.0%
!! TAAATAAATTGAGCTATTTA+TGG + tig0010653:70477-70496 None:intergenic 15.0%
!! ATTTAATTGTGAATCTAAGA+AGG + tig0010653:69184-69203 None:intergenic 20.0%
!! TCAGATTAGTAAAAGAAATT+GGG + tig0010653:69237-69256 None:intergenic 20.0%
!! ATCAGATTAGTAAAAGAAAT+TGG + tig0010653:69238-69257 None:intergenic 20.0%
!!! ATTTCTCTATTTTTGTGTTT+CGG - tig0010653:69300-69319 Msa1349100:intron 20.0%
!!! GTTTTTCAGAAGTTAATAAA+TGG - tig0010653:69437-69456 Msa1349100:intron 20.0%
!! AGAAAACAACAAAAAACTTA+AGG + tig0010653:69625-69644 None:intergenic 20.0%
!! TAAGTATACTAAGATAAGAT+AGG + tig0010653:69697-69716 None:intergenic 20.0%
!!! TTTCATTAAATCTTTTGTTG+TGG - tig0010653:69815-69834 Msa1349100:intron 20.0%
!! ATAGTAGAATTCATATATTG+TGG + tig0010653:70061-70080 None:intergenic 20.0%
!! AGGTTCAAAAGTTAAAAATT+GGG + tig0010653:70160-70179 None:intergenic 20.0%
!! TAGGTTCAAAAGTTAAAAAT+TGG + tig0010653:70161-70180 None:intergenic 20.0%
!! TTTCAAATTATATGACATTC+AGG - tig0010653:70188-70207 Msa1349100:intron 20.0%
!! CATATAAAACTTCAATATTG+TGG + tig0010653:70366-70385 None:intergenic 20.0%
!!! TAGAATTTTGAATATTGACA+GGG + tig0010653:70589-70608 None:intergenic 20.0%
!!! ATAGAATTTTGAATATTGAC+AGG + tig0010653:70590-70609 None:intergenic 20.0%
!!! ATTATTTTATGCATTGTTGT+AGG - tig0010653:70608-70627 Msa1349100:intron 20.0%
!! ATTTAATTGTGAATCTAAGA+AGG + tig0010653:69184-69203 None:intergenic 20.0%
!! TCAGATTAGTAAAAGAAATT+GGG + tig0010653:69237-69256 None:intergenic 20.0%
!! ATCAGATTAGTAAAAGAAAT+TGG + tig0010653:69238-69257 None:intergenic 20.0%
!!! ATTTCTCTATTTTTGTGTTT+CGG - tig0010653:69300-69319 Msa1349100:intron 20.0%
!!! GTTTTTCAGAAGTTAATAAA+TGG - tig0010653:69437-69456 Msa1349100:intron 20.0%
!! AGAAAACAACAAAAAACTTA+AGG + tig0010653:69625-69644 None:intergenic 20.0%
!! TAAGTATACTAAGATAAGAT+AGG + tig0010653:69697-69716 None:intergenic 20.0%
!!! TTTCATTAAATCTTTTGTTG+TGG - tig0010653:69815-69834 Msa1349100:intron 20.0%
!! ATAGTAGAATTCATATATTG+TGG + tig0010653:70061-70080 None:intergenic 20.0%
!! AGGTTCAAAAGTTAAAAATT+GGG + tig0010653:70160-70179 None:intergenic 20.0%
!! TAGGTTCAAAAGTTAAAAAT+TGG + tig0010653:70161-70180 None:intergenic 20.0%
!! TTTCAAATTATATGACATTC+AGG - tig0010653:70188-70207 Msa1349100:intron 20.0%
!! CATATAAAACTTCAATATTG+TGG + tig0010653:70366-70385 None:intergenic 20.0%
!!! TAGAATTTTGAATATTGACA+GGG + tig0010653:70589-70608 None:intergenic 20.0%
!!! ATAGAATTTTGAATATTGAC+AGG + tig0010653:70590-70609 None:intergenic 20.0%
!!! ATTATTTTATGCATTGTTGT+AGG - tig0010653:70608-70627 Msa1349100:intron 20.0%
! TAGGGTTTCACATAAAAATT+GGG + tig0010653:69213-69232 None:intergenic 25.0%
! TTAGGGTTTCACATAAAAAT+TGG + tig0010653:69214-69233 None:intergenic 25.0%
! TAGTAAAAGAAATTGGGTTA+GGG + tig0010653:69231-69250 None:intergenic 25.0%
! TTAGTAAAAGAAATTGGGTT+AGG + tig0010653:69232-69251 None:intergenic 25.0%
!! TTCTGAAAAACAAGTTATCA+GGG + tig0010653:69428-69447 None:intergenic 25.0%
! TTTCAGAAGTTAATAAATGG+TGG - tig0010653:69440-69459 Msa1349100:intron 25.0%
! ACCTTTGATTAATAGTTTAG+TGG - tig0010653:69743-69762 Msa1349100:CDS 25.0%
! CTATACTAGTGAATTACATA+AGG - tig0010653:69877-69896 Msa1349100:intron 25.0%
! GAAAAAGAAAAAATGAAGAG+AGG + tig0010653:69955-69974 None:intergenic 25.0%
! GGTTCAAAAGTTAAAAATTG+GGG + tig0010653:70159-70178 None:intergenic 25.0%
!! TTTTTCTGGATAAATATAGC+AGG + tig0010653:70425-70444 None:intergenic 25.0%
!!! CTTCAAATCATGTTTTTTTC+TGG + tig0010653:70439-70458 None:intergenic 25.0%
! TTTGTTTATACTCCATTTCA+TGG - tig0010653:70641-70660 Msa1349100:intron 25.0%
! AAAATGTAGTACAAAACATG+AGG + tig0010653:70685-70704 None:intergenic 25.0%
!! CATTTTTGACAATTCTGTAT+TGG - tig0010653:70699-70718 Msa1349100:five_prime_UTR 25.0%
! TAGGGTTTCACATAAAAATT+GGG + tig0010653:69213-69232 None:intergenic 25.0%
! TTAGGGTTTCACATAAAAAT+TGG + tig0010653:69214-69233 None:intergenic 25.0%
! TAGTAAAAGAAATTGGGTTA+GGG + tig0010653:69231-69250 None:intergenic 25.0%
! TTAGTAAAAGAAATTGGGTT+AGG + tig0010653:69232-69251 None:intergenic 25.0%
!! TTCTGAAAAACAAGTTATCA+GGG + tig0010653:69428-69447 None:intergenic 25.0%
! TTTCAGAAGTTAATAAATGG+TGG - tig0010653:69440-69459 Msa1349100:intron 25.0%
! ACCTTTGATTAATAGTTTAG+TGG - tig0010653:69743-69762 Msa1349100:CDS 25.0%
! CTATACTAGTGAATTACATA+AGG - tig0010653:69877-69896 Msa1349100:intron 25.0%
! GAAAAAGAAAAAATGAAGAG+AGG + tig0010653:69955-69974 None:intergenic 25.0%
! GGTTCAAAAGTTAAAAATTG+GGG + tig0010653:70159-70178 None:intergenic 25.0%
!! TTTTTCTGGATAAATATAGC+AGG + tig0010653:70425-70444 None:intergenic 25.0%
!!! CTTCAAATCATGTTTTTTTC+TGG + tig0010653:70439-70458 None:intergenic 25.0%
! TTTGTTTATACTCCATTTCA+TGG - tig0010653:70641-70660 Msa1349100:intron 25.0%
! AAAATGTAGTACAAAACATG+AGG + tig0010653:70685-70704 None:intergenic 25.0%
!! CATTTTTGACAATTCTGTAT+TGG - tig0010653:70699-70718 Msa1349100:five_prime_UTR 25.0%
TGCAAAAACACAGAAATGAA+CGG + tig0010653:69043-69062 None:intergenic 30.0%
ATAAGAGAAAGAAGATAACG+AGG + tig0010653:69070-69089 None:intergenic 30.0%
AGGGTTTCACATAAAAATTG+GGG + tig0010653:69212-69231 None:intergenic 30.0%
!!! TTTGTGTTTCGGTACTTTTT+TGG - tig0010653:69311-69330 Msa1349100:intron 30.0%
!!! TTGTGTTTCGGTACTTTTTT+GGG - tig0010653:69312-69331 Msa1349100:intron 30.0%
! CTTCTGAAAAACAAGTTATC+AGG + tig0010653:69429-69448 None:intergenic 30.0%
TAGTTGAAAGAATATTGCAG+AGG + tig0010653:69533-69552 None:intergenic 30.0%
GCAATATTCTTTCAACTAAC+TGG - tig0010653:69535-69554 Msa1349100:intron 30.0%
GTCACTGAACTATTAATCAA+AGG + tig0010653:69661-69680 None:intergenic 30.0%
ACTAAGATAAGATAGCCTAT+AGG + tig0010653:69690-69709 None:intergenic 30.0%
GCCACTAAACTATTAATCAA+AGG + tig0010653:69747-69766 None:intergenic 30.0%
!! CTTTTGTTGTGGAAGTAATA+CGG - tig0010653:69826-69845 Msa1349100:intron 30.0%
!! TTTTGTTGTGGAAGTAATAC+GGG - tig0010653:69827-69846 Msa1349100:intron 30.0%
ATTTGACAGCATGATTATCA+TGG + tig0010653:69853-69872 None:intergenic 30.0%
AAAGAAAAAATGAAGAGAGG+TGG + tig0010653:69952-69971 None:intergenic 30.0%
TATGAATTCTACTATCGATG+TGG - tig0010653:70066-70085 Msa1349100:intron 30.0%
TTCAGGAAGATTGAGTTATT+AGG - tig0010653:70205-70224 Msa1349100:CDS 30.0%
TCAGGAAGATTGAGTTATTA+GGG - tig0010653:70206-70225 Msa1349100:CDS 30.0%
TATTTATTCTGTTGAACCTG+CGG - tig0010653:70491-70510 Msa1349100:intron 30.0%
TGCAAAAACACAGAAATGAA+CGG + tig0010653:69043-69062 None:intergenic 30.0%
ATAAGAGAAAGAAGATAACG+AGG + tig0010653:69070-69089 None:intergenic 30.0%
AGGGTTTCACATAAAAATTG+GGG + tig0010653:69212-69231 None:intergenic 30.0%
!!! TTTGTGTTTCGGTACTTTTT+TGG - tig0010653:69311-69330 Msa1349100:intron 30.0%
!!! TTGTGTTTCGGTACTTTTTT+GGG - tig0010653:69312-69331 Msa1349100:intron 30.0%
! CTTCTGAAAAACAAGTTATC+AGG + tig0010653:69429-69448 None:intergenic 30.0%
TAGTTGAAAGAATATTGCAG+AGG + tig0010653:69533-69552 None:intergenic 30.0%
GCAATATTCTTTCAACTAAC+TGG - tig0010653:69535-69554 Msa1349100:intron 30.0%
GTCACTGAACTATTAATCAA+AGG + tig0010653:69661-69680 None:intergenic 30.0%
ACTAAGATAAGATAGCCTAT+AGG + tig0010653:69690-69709 None:intergenic 30.0%
GCCACTAAACTATTAATCAA+AGG + tig0010653:69747-69766 None:intergenic 30.0%
!! CTTTTGTTGTGGAAGTAATA+CGG - tig0010653:69826-69845 Msa1349100:intron 30.0%
!! TTTTGTTGTGGAAGTAATAC+GGG - tig0010653:69827-69846 Msa1349100:intron 30.0%
ATTTGACAGCATGATTATCA+TGG + tig0010653:69853-69872 None:intergenic 30.0%
AAAGAAAAAATGAAGAGAGG+TGG + tig0010653:69952-69971 None:intergenic 30.0%
TATGAATTCTACTATCGATG+TGG - tig0010653:70066-70085 Msa1349100:intron 30.0%
TTCAGGAAGATTGAGTTATT+AGG - tig0010653:70205-70224 Msa1349100:CDS 30.0%
TCAGGAAGATTGAGTTATTA+GGG - tig0010653:70206-70225 Msa1349100:CDS 30.0%
TATTTATTCTGTTGAACCTG+CGG - tig0010653:70491-70510 Msa1349100:intron 30.0%
CGGATTGAAATCGAAAATCA+GGG + tig0010653:68949-68968 None:intergenic 35.0%
! CCTGATTTTCGATTTCAATC+CGG - tig0010653:68947-68966 Msa1349100:CDS 35.0%
!! TTTTTCAGTTTATGACGTCG+AGG + tig0010653:68976-68995 None:intergenic 35.0%
ATTGAGTCTCTCTTCTTCAA+GGG - tig0010653:69143-69162 Msa1349100:intron 35.0%
! ACTTGGTGAAACACCATTAT+CGG + tig0010653:69574-69593 None:intergenic 35.0%
AATAGAAGACTAACCCTTGA+AGG + tig0010653:70100-70119 None:intergenic 35.0%
! GTTATTTAGGGTCACTAGTT+AGG - tig0010653:70282-70301 Msa1349100:CDS 35.0%
! TAGTTAGGAATCCTCAGTTT+TGG - tig0010653:70297-70316 Msa1349100:exon 35.0%
! AGTTAGGAATCCTCAGTTTT+GGG - tig0010653:70298-70317 Msa1349100:exon 35.0%
TAGCAGGTTCATAGCAAATA+TGG + tig0010653:70409-70428 None:intergenic 35.0%
CTTATGATAGCTTAGTCTCA+AGG + tig0010653:70547-70566 None:intergenic 35.0%
ACTAGTACTCTACCATGAAA+TGG + tig0010653:70656-70675 None:intergenic 35.0%
! TTTGACAATTCTGTATTGGC+AGG - tig0010653:70703-70722 Msa1349100:five_prime_UTR 35.0%
!!! CTTCCTTGTTTTATCCTTGT+CGG + tig0010653:70749-70768 None:intergenic 35.0%
CGGATTGAAATCGAAAATCA+GGG + tig0010653:68949-68968 None:intergenic 35.0%
! CCTGATTTTCGATTTCAATC+CGG - tig0010653:68947-68966 Msa1349100:CDS 35.0%
!! TTTTTCAGTTTATGACGTCG+AGG + tig0010653:68976-68995 None:intergenic 35.0%
ATTGAGTCTCTCTTCTTCAA+GGG - tig0010653:69143-69162 Msa1349100:intron 35.0%
! ACTTGGTGAAACACCATTAT+CGG + tig0010653:69574-69593 None:intergenic 35.0%
AATAGAAGACTAACCCTTGA+AGG + tig0010653:70100-70119 None:intergenic 35.0%
! GTTATTTAGGGTCACTAGTT+AGG - tig0010653:70282-70301 Msa1349100:CDS 35.0%
! TAGTTAGGAATCCTCAGTTT+TGG - tig0010653:70297-70316 Msa1349100:exon 35.0%
! AGTTAGGAATCCTCAGTTTT+GGG - tig0010653:70298-70317 Msa1349100:exon 35.0%
TAGCAGGTTCATAGCAAATA+TGG + tig0010653:70409-70428 None:intergenic 35.0%
CTTATGATAGCTTAGTCTCA+AGG + tig0010653:70547-70566 None:intergenic 35.0%
ACTAGTACTCTACCATGAAA+TGG + tig0010653:70656-70675 None:intergenic 35.0%
! TTTGACAATTCTGTATTGGC+AGG - tig0010653:70703-70722 Msa1349100:five_prime_UTR 35.0%
!!! CTTCCTTGTTTTATCCTTGT+CGG + tig0010653:70749-70768 None:intergenic 35.0%
!!! CTGAATGTGAGGGTTTTGTT+TGG + tig0010653:68926-68945 None:intergenic 40.0%
AAAATCAGGGCTGAATGTGA+GGG + tig0010653:68936-68955 None:intergenic 40.0%
CCGGATTGAAATCGAAAATC+AGG + tig0010653:68950-68969 None:intergenic 40.0%
CTTCTTTCTCTTATCCCTCT+TGG - tig0010653:69076-69095 Msa1349100:intron 40.0%
CATTGAGTCTCTCTTCTTCA+AGG - tig0010653:69142-69161 Msa1349100:intron 40.0%
TTTGGGCCTATCTCTGTTTA+CGG - tig0010653:69329-69348 Msa1349100:intron 40.0%
CAATCACCGTAAACAGAGAT+AGG + tig0010653:69338-69357 None:intergenic 40.0%
TGAGCCATACCATTGTCAAT+CGG + tig0010653:69599-69618 None:intergenic 40.0%
TTCAGTGACTCAACACCTAT+AGG - tig0010653:69672-69691 Msa1349100:intron 40.0%
GAAGGAACTCAGAACAAAGA+AGG + tig0010653:69993-70012 None:intergenic 40.0%
TATCATTGTGCCCAAAACTG+AGG + tig0010653:70311-70330 None:intergenic 40.0%
! TGTATTGGCAGGAGCAAAAT+TGG - tig0010653:70714-70733 Msa1349100:five_prime_UTR 40.0%
! ATTGGTAATCAAAGCCGACA+AGG - tig0010653:70732-70751 Msa1349100:five_prime_UTR 40.0%
AAGCCGACAAGGATAAAACA+AGG - tig0010653:70743-70762 Msa1349100:five_prime_UTR 40.0%
!! CAACGAATAAAAAGCGTCGT+AGG + tig0010653:70846-70865 None:intergenic 40.0%
!! CGACGCTTTTTATTCGTTGA+AGG - tig0010653:70847-70866 Msa1349100:five_prime_UTR 40.0%
!!! CTGAATGTGAGGGTTTTGTT+TGG + tig0010653:68926-68945 None:intergenic 40.0%
AAAATCAGGGCTGAATGTGA+GGG + tig0010653:68936-68955 None:intergenic 40.0%
CCGGATTGAAATCGAAAATC+AGG + tig0010653:68950-68969 None:intergenic 40.0%
CTTCTTTCTCTTATCCCTCT+TGG - tig0010653:69076-69095 Msa1349100:intron 40.0%
CATTGAGTCTCTCTTCTTCA+AGG - tig0010653:69142-69161 Msa1349100:intron 40.0%
TTTGGGCCTATCTCTGTTTA+CGG - tig0010653:69329-69348 Msa1349100:intron 40.0%
CAATCACCGTAAACAGAGAT+AGG + tig0010653:69338-69357 None:intergenic 40.0%
TGAGCCATACCATTGTCAAT+CGG + tig0010653:69599-69618 None:intergenic 40.0%
TTCAGTGACTCAACACCTAT+AGG - tig0010653:69672-69691 Msa1349100:intron 40.0%
GAAGGAACTCAGAACAAAGA+AGG + tig0010653:69993-70012 None:intergenic 40.0%
TATCATTGTGCCCAAAACTG+AGG + tig0010653:70311-70330 None:intergenic 40.0%
! TGTATTGGCAGGAGCAAAAT+TGG - tig0010653:70714-70733 Msa1349100:five_prime_UTR 40.0%
! ATTGGTAATCAAAGCCGACA+AGG - tig0010653:70732-70751 Msa1349100:five_prime_UTR 40.0%
AAGCCGACAAGGATAAAACA+AGG - tig0010653:70743-70762 Msa1349100:five_prime_UTR 40.0%
!! CAACGAATAAAAAGCGTCGT+AGG + tig0010653:70846-70865 None:intergenic 40.0%
!! CGACGCTTTTTATTCGTTGA+AGG - tig0010653:70847-70866 Msa1349100:five_prime_UTR 40.0%
!!! TGTGAGGGTTTTGTTTGGAG+AGG + tig0010653:68921-68940 None:intergenic 45.0%
GAAAATCAGGGCTGAATGTG+AGG + tig0010653:68937-68956 None:intergenic 45.0%
TTCAGTTTATGACGTCGAGG+TGG + tig0010653:68973-68992 None:intergenic 45.0%
CAGAGAAGTCGTTACCAAGA+GGG + tig0010653:69093-69112 None:intergenic 45.0%
GAAGAGAGACTCAATGACGT+CGG + tig0010653:69138-69157 None:intergenic 45.0%
AATAGCGACGACTCCGATAA+TGG - tig0010653:69558-69577 Msa1349100:intron 45.0%
ACCAAGTCTCCGATTGACAA+TGG - tig0010653:69587-69606 Msa1349100:intron 45.0%
ACCATTGTCAATCGGAGACT+TGG + tig0010653:69591-69610 None:intergenic 45.0%
GTCTCCGATTGACAATGGTA+TGG - tig0010653:69592-69611 Msa1349100:intron 45.0%
CACAAATGGTCTAACCACAG+AGG + tig0010653:69928-69947 None:intergenic 45.0%
TGAAGAGAGGTGGTCACAAA+TGG + tig0010653:69942-69961 None:intergenic 45.0%
!! GAGACCTATTTGAGAGCATC+CGG - tig0010653:70802-70821 Msa1349100:five_prime_UTR 45.0%
TCGTAGGACATCCTCTTGTT+GGG + tig0010653:70830-70849 None:intergenic 45.0%
AGGCTTGAATGTATTCTCCC+CGG - tig0010653:70867-70886 Msa1349100:five_prime_UTR 45.0%
!!! TGTGAGGGTTTTGTTTGGAG+AGG + tig0010653:68921-68940 None:intergenic 45.0%
GAAAATCAGGGCTGAATGTG+AGG + tig0010653:68937-68956 None:intergenic 45.0%
TTCAGTTTATGACGTCGAGG+TGG + tig0010653:68973-68992 None:intergenic 45.0%
CAGAGAAGTCGTTACCAAGA+GGG + tig0010653:69093-69112 None:intergenic 45.0%
GAAGAGAGACTCAATGACGT+CGG + tig0010653:69138-69157 None:intergenic 45.0%
AATAGCGACGACTCCGATAA+TGG - tig0010653:69558-69577 Msa1349100:intron 45.0%
ACCAAGTCTCCGATTGACAA+TGG - tig0010653:69587-69606 Msa1349100:intron 45.0%
ACCATTGTCAATCGGAGACT+TGG + tig0010653:69591-69610 None:intergenic 45.0%
GTCTCCGATTGACAATGGTA+TGG - tig0010653:69592-69611 Msa1349100:intron 45.0%
CACAAATGGTCTAACCACAG+AGG + tig0010653:69928-69947 None:intergenic 45.0%
TGAAGAGAGGTGGTCACAAA+TGG + tig0010653:69942-69961 None:intergenic 45.0%
!! GAGACCTATTTGAGAGCATC+CGG - tig0010653:70802-70821 Msa1349100:five_prime_UTR 45.0%
TCGTAGGACATCCTCTTGTT+GGG + tig0010653:70830-70849 None:intergenic 45.0%
AGGCTTGAATGTATTCTCCC+CGG - tig0010653:70867-70886 Msa1349100:five_prime_UTR 45.0%
CCAGAGAAGTCGTTACCAAG+AGG + tig0010653:69094-69113 None:intergenic 50.0%
!! CCTCTTGGTAACGACTTCTC+TGG - tig0010653:69091-69110 Msa1349100:intron 50.0%
AACCACAGAGGAGTAGAGGA+GGG + tig0010653:69916-69935 None:intergenic 50.0%
TAACCACAGAGGAGTAGAGG+AGG + tig0010653:69917-69936 None:intergenic 50.0%
GTCTAACCACAGAGGAGTAG+AGG + tig0010653:69920-69939 None:intergenic 50.0%
!! ATGTGGTGTCGTCTCCTTCA+AGG - tig0010653:70083-70102 Msa1349100:intron 50.0%
!! TGTGGTGTCGTCTCCTTCAA+GGG - tig0010653:70084-70103 Msa1349100:intron 50.0%
! ATATTTTCCCGAGACTCCGC+AGG + tig0010653:70510-70529 None:intergenic 50.0%
! GGTACCGGATGCTCTCAAAT+AGG + tig0010653:70809-70828 None:intergenic 50.0%
GACATCCTCTTGTTGGGTAC+CGG + tig0010653:70824-70843 None:intergenic 50.0%
GTCGTAGGACATCCTCTTGT+TGG + tig0010653:70831-70850 None:intergenic 50.0%
GGCTTGAATGTATTCTCCCC+GGG - tig0010653:70868-70887 Msa1349100:five_prime_UTR 50.0%
CCAGAGAAGTCGTTACCAAG+AGG + tig0010653:69094-69113 None:intergenic 50.0%
!! CCTCTTGGTAACGACTTCTC+TGG - tig0010653:69091-69110 Msa1349100:intron 50.0%
AACCACAGAGGAGTAGAGGA+GGG + tig0010653:69916-69935 None:intergenic 50.0%
TAACCACAGAGGAGTAGAGG+AGG + tig0010653:69917-69936 None:intergenic 50.0%
GTCTAACCACAGAGGAGTAG+AGG + tig0010653:69920-69939 None:intergenic 50.0%
!! ATGTGGTGTCGTCTCCTTCA+AGG - tig0010653:70083-70102 Msa1349100:intron 50.0%
!! TGTGGTGTCGTCTCCTTCAA+GGG - tig0010653:70084-70103 Msa1349100:intron 50.0%
! ATATTTTCCCGAGACTCCGC+AGG + tig0010653:70510-70529 None:intergenic 50.0%
! GGTACCGGATGCTCTCAAAT+AGG + tig0010653:70809-70828 None:intergenic 50.0%
GACATCCTCTTGTTGGGTAC+CGG + tig0010653:70824-70843 None:intergenic 50.0%
GTCGTAGGACATCCTCTTGT+TGG + tig0010653:70831-70850 None:intergenic 50.0%
GGCTTGAATGTATTCTCCCC+GGG - tig0010653:70868-70887 Msa1349100:five_prime_UTR 50.0%
GTTTATGACGTCGAGGTGGC+CGG + tig0010653:68969-68988 None:intergenic 55.0%
!! CTTCTCTGGAACCACCACAC+CGG - tig0010653:69105-69124 Msa1349100:intron 55.0%
!! TTCTCTGGAACCACCACACC+GGG - tig0010653:69106-69125 Msa1349100:intron 55.0%
CAATGACGTCGGAATGACCC+CGG + tig0010653:69127-69146 None:intergenic 55.0%
CTGTTGAACCTGCGGAGTCT+CGG - tig0010653:70499-70518 Msa1349100:intron 55.0%
TGTTGAACCTGCGGAGTCTC+GGG - tig0010653:70500-70519 Msa1349100:intron 55.0%
AGCATCCGGTACCCAACAAG+AGG - tig0010653:70816-70835 Msa1349100:five_prime_UTR 55.0%
GTATTCTCCCCGGGTTCTAC+TGG - tig0010653:70877-70896 Msa1349100:five_prime_UTR 55.0%
GTTTATGACGTCGAGGTGGC+CGG + tig0010653:68969-68988 None:intergenic 55.0%
!! CTTCTCTGGAACCACCACAC+CGG - tig0010653:69105-69124 Msa1349100:intron 55.0%
!! TTCTCTGGAACCACCACACC+GGG - tig0010653:69106-69125 Msa1349100:intron 55.0%
CAATGACGTCGGAATGACCC+CGG + tig0010653:69127-69146 None:intergenic 55.0%
CTGTTGAACCTGCGGAGTCT+CGG - tig0010653:70499-70518 Msa1349100:intron 55.0%
TGTTGAACCTGCGGAGTCTC+GGG - tig0010653:70500-70519 Msa1349100:intron 55.0%
AGCATCCGGTACCCAACAAG+AGG - tig0010653:70816-70835 Msa1349100:five_prime_UTR 55.0%
GTATTCTCCCCGGGTTCTAC+TGG - tig0010653:70877-70896 Msa1349100:five_prime_UTR 55.0%
!! TCTCTGGAACCACCACACCG+GGG - tig0010653:69107-69126 Msa1349100:intron 60.0%
CACCCTCCTCTACTCCTCTG+TGG - tig0010653:69911-69930 Msa1349100:intron 60.0%
!! TCTCTGGAACCACCACACCG+GGG - tig0010653:69107-69126 Msa1349100:intron 60.0%
CACCCTCCTCTACTCCTCTG+TGG - tig0010653:69911-69930 Msa1349100:intron 60.0%
TCGGAATGACCCCGGTGTGG+TGG + tig0010653:69119-69138 None:intergenic 65.0%
ACGTCGGAATGACCCCGGTG+TGG + tig0010653:69122-69141 None:intergenic 65.0%
TCGGAATGACCCCGGTGTGG+TGG + tig0010653:69119-69138 None:intergenic 65.0%
ACGTCGGAATGACCCCGGTG+TGG + tig0010653:69122-69141 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0010653 gene 68915 70903 68915 ID=Msa1349100;Name=Msa1349100
tig0010653 mRNA 68915 70903 68915 ID=Msa1349100-mRNA-1;Parent=Msa1349100;Name=Msa1349100-mRNA-1;_AED=0.27;_eAED=0.27;_QI=259|0.66|0.25|1|0|0.5|4|0|122
tig0010653 exon 68915 69093 68915 ID=Msa1349100-mRNA-1:exon:6699;Parent=Msa1349100-mRNA-1
tig0010653 exon 69713 69781 69713 ID=Msa1349100-mRNA-1:exon:6698;Parent=Msa1349100-mRNA-1
tig0010653 exon 70191 70320 70191 ID=Msa1349100-mRNA-1:exon:6697;Parent=Msa1349100-mRNA-1
tig0010653 exon 70654 70903 70654 ID=Msa1349100-mRNA-1:exon:6696;Parent=Msa1349100-mRNA-1
tig0010653 five_prime_UTR 70654 70903 70654 ID=Msa1349100-mRNA-1:five_prime_utr;Parent=Msa1349100-mRNA-1
tig0010653 five_prime_UTR 70312 70320 70312 ID=Msa1349100-mRNA-1:five_prime_utr;Parent=Msa1349100-mRNA-1
tig0010653 CDS 70191 70311 70191 ID=Msa1349100-mRNA-1:cds;Parent=Msa1349100-mRNA-1
tig0010653 CDS 69713 69781 69713 ID=Msa1349100-mRNA-1:cds;Parent=Msa1349100-mRNA-1
tig0010653 CDS 68915 69093 68915 ID=Msa1349100-mRNA-1:cds;Parent=Msa1349100-mRNA-1
Gene Sequence

>Msa1349100

ATGTCAGAGACAACTCTAAAGAACCTCTGCAATATTCTTTCAACTAACTGGAATAGCGACGACTCCGATAATGGTGTTTCACCAAGTCTCCGATTGACAATGGTATGGCTCAATACCTTAAATTCAATTTTTTTTTACAGAACCACAATATATGAATTCTACTATCGATGTGGTGTCGTCTCCTTCAAGGGAGCAAAATTGGTAATCAAAGCCGACAAGGATAAAACAAGGAAGATCGAATTCTGCTACATTCAGTCTAATGTTGTTGAGACCTATTTGAGAGCATCCGGTACCCAACAAGAGGATGTCCTACGACGCTTTTTATTCGTTGAAGGCTTGAATGTATTCTCCCCGGGTTCTACTGGTTAG
Protein sequence

>Msa1349100

MSETTLKNLCNILSTNWNSDDSDNGVSPSLRLTMVWLNTLNSIFFYRTTIYEFYYRCGVVSFKGAKLVIKADKDKTRKIEFCYIQSNVVETYLRASGTQQEDVLRRFLFVEGLNVFSPGSTG*