Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0054250 | Msa1351090 | 0.822916 | 1.833469e-53 | -8.615850e-47 |
| Msa0076670 | Msa1351090 | 0.801556 | 8.517760e-49 | -8.615850e-47 |
| Msa0078610 | Msa1351090 | 0.814750 | 1.314423e-51 | -8.615850e-47 |
| Msa0100080 | Msa1351090 | 0.811322 | 7.419368e-51 | -8.615850e-47 |
| Msa1351090 | Msa1392940 | 0.828306 | 9.676070e-55 | -8.615850e-47 |
| Msa0220450 | Msa1351090 | 0.810800 | 9.625495e-51 | -8.615850e-47 |
| Msa1247920 | Msa1351090 | 0.804795 | 1.819358e-49 | -8.615850e-47 |
| Msa0378380 | Msa1351090 | 0.822848 | 1.901478e-53 | -8.615850e-47 |
| Msa0427730 | Msa1351090 | 0.811264 | 7.638798e-51 | -8.615850e-47 |
| Msa0427980 | Msa1351090 | 0.832025 | 1.196280e-55 | -8.615850e-47 |
| Msa1006630 | Msa1351090 | 0.818726 | 1.686063e-52 | -8.615850e-47 |
| Msa0518560 | Msa1351090 | 0.817530 | 3.144259e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1351090 | MtrunA17_Chr3g0083351 | 52.778 | 72 | 30 | 3 | 1 | 70 | 15 | 84 | 1.31e-13 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCCGCGCAGGGATCGAATT+CGG | 0.296686 | tig0012121:-8348 | None:intergenic |
| TCCGCGCAGGGATCGAATTC+GGG | 0.341695 | tig0012121:-8347 | None:intergenic |
| GCTGGGCGAGGTTTGAATAT+TGG | 0.374204 | tig0012121:-8302 | None:intergenic |
| ATCCCTGCGCGGAGGAACTC+GGG | 0.478074 | tig0012121:+8357 | Msa1351090:CDS |
| GATCCCTGCGCGGAGGAACT+CGG | 0.500670 | tig0012121:+8356 | Msa1351090:CDS |
| TTTAGGAACGTTTAGGTGGA+GGG | 0.514244 | tig0012121:-8400 | None:intergenic |
| CTTCCCGAGTTCCTCCGCGC+AGG | 0.526240 | tig0012121:-8360 | None:intergenic |
| GGGCGAGGTTTGAATATTGG+AGG | 0.534593 | tig0012121:-8299 | None:intergenic |
| TTCCCGAGTTCCTCCGCGCA+GGG | 0.540712 | tig0012121:-8359 | None:intergenic |
| GGCGAGGTTTGAATATTGGA+GGG | 0.603093 | tig0012121:-8298 | None:intergenic |
| GCAGCATCAGCAAAATGTTG+AGG | 0.624488 | tig0012121:-8266 | None:intergenic |
| GCCCGAATTCGATCCCTGCG+CGG | 0.698606 | tig0012121:+8346 | Msa1351090:CDS |
| GAACAAACATAAGAGCAGCA+AGG | 0.711648 | tig0012121:-8244 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAATTTAGGGAATTTTGCT+GGG | - | tig0012121:8322-8341 | None:intergenic | 25.0% |
| !!! | TTTAATTTAGGGAATTTTGC+TGG | - | tig0012121:8323-8342 | None:intergenic | 25.0% |
| !!! | ATTCGGGCTTTTTTAATTTA+GGG | - | tig0012121:8334-8353 | None:intergenic | 25.0% |
| !!! | AATTCGGGCTTTTTTAATTT+AGG | - | tig0012121:8335-8354 | None:intergenic | 25.0% |
| ! | AGCAAGGAACAACACTTTTA+AGG | - | tig0012121:8231-8250 | None:intergenic | 35.0% |
| GAACAAACATAAGAGCAGCA+AGG | - | tig0012121:8247-8266 | None:intergenic | 40.0% | |
| GCAGCATCAGCAAAATGTTG+AGG | - | tig0012121:8269-8288 | None:intergenic | 45.0% | |
| GGCGAGGTTTGAATATTGGA+GGG | - | tig0012121:8301-8320 | None:intergenic | 45.0% | |
| GGGCGAGGTTTGAATATTGG+AGG | - | tig0012121:8302-8321 | None:intergenic | 50.0% | |
| GCTGGGCGAGGTTTGAATAT+TGG | - | tig0012121:8305-8324 | None:intergenic | 50.0% | |
| ! | TTAGGGAATTTTGCTGGGCG+AGG | - | tig0012121:8317-8336 | None:intergenic | 50.0% |
| TCCGCGCAGGGATCGAATTC+GGG | - | tig0012121:8350-8369 | None:intergenic | 60.0% | |
| CTCCGCGCAGGGATCGAATT+CGG | - | tig0012121:8351-8370 | None:intergenic | 60.0% | |
| GCCCGAATTCGATCCCTGCG+CGG | + | tig0012121:8346-8365 | Msa1351090:CDS | 65.0% | |
| CGAATTCGATCCCTGCGCGG+AGG | + | tig0012121:8349-8368 | Msa1351090:CDS | 65.0% | |
| GATCCCTGCGCGGAGGAACT+CGG | + | tig0012121:8356-8375 | Msa1351090:CDS | 65.0% | |
| ATCCCTGCGCGGAGGAACTC+GGG | + | tig0012121:8357-8376 | Msa1351090:CDS | 65.0% | |
| TTCCCGAGTTCCTCCGCGCA+GGG | - | tig0012121:8362-8381 | None:intergenic | 65.0% | |
| CTTCCCGAGTTCCTCCGCGC+AGG | - | tig0012121:8363-8382 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0012121 | gene | 8206 | 8421 | 8206 | ID=Msa1351090;Name=Msa1351090 |
| tig0012121 | mRNA | 8206 | 8421 | 8206 | ID=Msa1351090-mRNA-1;Parent=Msa1351090;Name=Msa1351090-mRNA-1;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|71 |
| tig0012121 | exon | 8206 | 8421 | 8206 | ID=Msa1351090-mRNA-1:exon:7651;Parent=Msa1351090-mRNA-1 |
| tig0012121 | CDS | 8206 | 8421 | 8206 | ID=Msa1351090-mRNA-1:cds;Parent=Msa1351090-mRNA-1 |
| Gene Sequence |
| Protein sequence |
