AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1361230


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1361230 MtrunA17_Chr5g0396771 86.957 92 7 2 1 88 1 91 1.29e-50 154
Msa1361230 MtrunA17_Chr2g0322831 53.968 63 24 2 17 79 20 77 1.20e-16 67.8
Msa1361230 MtrunA17_Chr4g0024181 43.836 73 40 1 9 81 2 73 7.34e-16 65.9
Msa1361230 MtrunA17_Chr2g0288361 49.020 51 25 1 31 81 25 74 1.96e-13 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1361230 AT2G30420.1 62.651 83 24 2 6 88 17 92 2.18e-28 99.0
Msa1361230 AT5G53200.1 69.231 65 19 1 24 88 22 85 3.14e-28 98.2
Msa1361230 AT2G46410.1 60.000 75 29 1 13 87 16 89 5.53e-27 94.7
Msa1361230 AT2G30424.1 58.974 78 30 2 12 88 12 88 7.93e-26 92.0
Msa1361230 AT2G30432.1 64.179 67 23 1 19 85 19 84 3.38e-25 90.1
Msa1361230 AT4G01060.1 55.128 78 34 1 1 78 1 77 3.75e-24 87.0
Msa1361230 AT4G01060.2 62.500 64 23 1 15 78 12 74 1.52e-23 85.5
Msa1361230 AT1G01380.1 73.913 46 11 1 33 78 32 76 9.33e-19 73.6
Msa1361230 AT4G01060.3 68.085 47 14 1 32 78 30 75 8.22e-18 70.9
Msa1361230 AT2G30424.3 53.333 60 24 2 12 70 12 68 7.68e-12 55.8

Find 31 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATCTTCACTTCTTTCTTT+TGG 0.236926 tig0017392:-54332 None:intergenic
CGGAGGTTTGACTTGTGTTT+CGG 0.239671 tig0017392:-53374 None:intergenic
CAGAAGAAATAGAGAGATTC+TGG 0.240435 tig0017392:+54281 Msa1361230:CDS
GGAGGTTTGACTTGTGTTTC+GGG 0.242590 tig0017392:-53373 None:intergenic
GGGAGATATTTGCCTTAGTT+TGG 0.291933 tig0017392:-53353 None:intergenic
CTAGCATACAAACTTTGTTT+TGG 0.300692 tig0017392:+54426 Msa1361230:three_prime_UTR
ACCACCAGGTAGATAAAATA+TGG 0.317616 tig0017392:+53320 None:intergenic
TGTCTGAAGCAAGCTTTATA+TGG 0.347994 tig0017392:+54499 Msa1361230:three_prime_UTR
TTGATAGCAGGTCGCATTCC+TGG 0.360830 tig0017392:+54250 Msa1361230:CDS
GGAGATATTTGCCTTAGTTT+GGG 0.373654 tig0017392:-53352 None:intergenic
TTTGTATTGCATCTCGTTGA+AGG 0.424176 tig0017392:+54463 Msa1361230:three_prime_UTR
CAATTATATCTCTCTCTCCT+AGG 0.480221 tig0017392:+54389 Msa1361230:three_prime_UTR
GGAAGAACGTTCCCAAACTA+AGG 0.483455 tig0017392:+53341 Msa1361230:CDS
TGTACGACCTAGTTGGAGAT+AGG 0.497484 tig0017392:+53673 Msa1361230:CDS
AGCAGGTGGAATTTGATAGC+AGG 0.532686 tig0017392:+54238 Msa1361230:intron
CGCAGGATGTACGACCTAGT+TGG 0.536525 tig0017392:+53666 Msa1361230:CDS
CAGAAGTGAGCAGCACTGAG+TGG 0.541606 tig0017392:+53604 Msa1361230:CDS
ATTTCTTCTGCTTTACGACC+AGG 0.542074 tig0017392:-54268 None:intergenic
AAAATCAAGTGTAGGACAAA+CGG 0.544304 tig0017392:-54573 None:intergenic
TAGAATAATCAATCATGCCT+AGG 0.562767 tig0017392:-54406 None:intergenic
AGAAGTGAGCAGCACTGAGT+GGG 0.563793 tig0017392:+53605 Msa1361230:CDS
AAGATAAAGCAAATTAAACT+AGG 0.566688 tig0017392:+54348 Msa1361230:exon
CATAGAGATGAGCGAGCAAG+AGG 0.568119 tig0017392:+53632 Msa1361230:CDS
TATAAAGCTTGCTTCAGACA+TGG 0.570675 tig0017392:-54496 None:intergenic
ATAAAGCTTGCTTCAGACAT+GGG 0.573902 tig0017392:-54495 None:intergenic
AAGAGGAGGATCTCATTCGC+AGG 0.581290 tig0017392:+53649 Msa1361230:CDS
ACAAAAGGAAAATCAAGTGT+AGG 0.581382 tig0017392:-54581 None:intergenic
AGAGATGAGCGAGCAAGAGG+AGG 0.612798 tig0017392:+53635 Msa1361230:CDS
AGATTAGAAACCACCTTCGG+AGG 0.612968 tig0017392:-53391 None:intergenic
TTAATTACCTATCTCCAACT+AGG 0.634724 tig0017392:-53680 None:intergenic
AACACAAGTCAAACCTCCGA+AGG 0.653483 tig0017392:+53378 Msa1361230:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAGCAATAGAGAAAATAA+TGG - tig0017392:53452-53471 None:intergenic 20.0%
!! AAACATTAAATACTAGTATG+AGG - tig0017392:53559-53578 None:intergenic 20.0%
!! TATGATACTAATTGAATGTT+TGG - tig0017392:53937-53956 None:intergenic 20.0%
!! TAATCTTATATTATTGTGTC+CGG - tig0017392:54073-54092 None:intergenic 20.0%
!!! CATATAAGCTTTTATATGAA+AGG + tig0017392:54189-54208 Msa1361230:intron 20.0%
!! AAGATAAAGCAAATTAAACT+AGG + tig0017392:54348-54367 Msa1361230:exon 20.0%
!!! TGTATTTTGTACATAGTTTA+AGG + tig0017392:54532-54551 Msa1361230:three_prime_UTR 20.0%
!! AAAAGCAATAGAGAAAATAA+TGG - tig0017392:53452-53471 None:intergenic 20.0%
!! AAACATTAAATACTAGTATG+AGG - tig0017392:53559-53578 None:intergenic 20.0%
!! TATGATACTAATTGAATGTT+TGG - tig0017392:53937-53956 None:intergenic 20.0%
!! TAATCTTATATTATTGTGTC+CGG - tig0017392:54073-54092 None:intergenic 20.0%
!!! CATATAAGCTTTTATATGAA+AGG + tig0017392:54189-54208 Msa1361230:intron 20.0%
!! AAGATAAAGCAAATTAAACT+AGG + tig0017392:54348-54367 Msa1361230:exon 20.0%
!!! TGTATTTTGTACATAGTTTA+AGG + tig0017392:54532-54551 Msa1361230:three_prime_UTR 20.0%
!! TACAGACAAAGTATTATACA+TGG - tig0017392:53706-53725 None:intergenic 25.0%
! GTTAGTTAATTCTAACAACT+TGG - tig0017392:53970-53989 None:intergenic 25.0%
! ATATGCTAAAAAGTAAACAC+CGG + tig0017392:54051-54070 Msa1361230:intron 25.0%
!!! TCTTTAAGTTGTATGATGTT+TGG + tig0017392:54129-54148 Msa1361230:intron 25.0%
!! TTATCTTCACTTCTTTCTTT+TGG - tig0017392:54335-54354 None:intergenic 25.0%
!! TACAGACAAAGTATTATACA+TGG - tig0017392:53706-53725 None:intergenic 25.0%
! GTTAGTTAATTCTAACAACT+TGG - tig0017392:53970-53989 None:intergenic 25.0%
! ATATGCTAAAAAGTAAACAC+CGG + tig0017392:54051-54070 Msa1361230:intron 25.0%
!!! TCTTTAAGTTGTATGATGTT+TGG + tig0017392:54129-54148 Msa1361230:intron 25.0%
!! TTATCTTCACTTCTTTCTTT+TGG - tig0017392:54335-54354 None:intergenic 25.0%
AGAGAAAATAATGGCATGTT+GGG - tig0017392:53443-53462 None:intergenic 30.0%
TAGAGAAAATAATGGCATGT+TGG - tig0017392:53444-53463 None:intergenic 30.0%
TTATACGTGTACCAATTAAG+AGG + tig0017392:53501-53520 Msa1361230:intron 30.0%
TTAATTACCTATCTCCAACT+AGG - tig0017392:53683-53702 None:intergenic 30.0%
AAAAAAACCTCAAACGTCAA+AGG - tig0017392:53731-53750 None:intergenic 30.0%
!! TAAGAAGCTTTGACGTTTTA+AGG + tig0017392:53755-53774 Msa1361230:intron 30.0%
! ATTCTAACAACTTGGTCTTT+AGG - tig0017392:53962-53981 None:intergenic 30.0%
ATAGAAAAACTGTGATTGTG+AGG + tig0017392:54003-54022 Msa1361230:intron 30.0%
! TTAAGTTGTATGATGTTTGG+TGG + tig0017392:54132-54151 Msa1361230:intron 30.0%
TAGAATAATCAATCATGCCT+AGG - tig0017392:54409-54428 None:intergenic 30.0%
!! CTAGCATACAAACTTTGTTT+TGG + tig0017392:54426-54445 Msa1361230:three_prime_UTR 30.0%
AAAATCAAGTGTAGGACAAA+CGG - tig0017392:54576-54595 None:intergenic 30.0%
AGAGAAAATAATGGCATGTT+GGG - tig0017392:53443-53462 None:intergenic 30.0%
TAGAGAAAATAATGGCATGT+TGG - tig0017392:53444-53463 None:intergenic 30.0%
TTATACGTGTACCAATTAAG+AGG + tig0017392:53501-53520 Msa1361230:intron 30.0%
TTAATTACCTATCTCCAACT+AGG - tig0017392:53683-53702 None:intergenic 30.0%
AAAAAAACCTCAAACGTCAA+AGG - tig0017392:53731-53750 None:intergenic 30.0%
!! TAAGAAGCTTTGACGTTTTA+AGG + tig0017392:53755-53774 Msa1361230:intron 30.0%
! ATTCTAACAACTTGGTCTTT+AGG - tig0017392:53962-53981 None:intergenic 30.0%
ATAGAAAAACTGTGATTGTG+AGG + tig0017392:54003-54022 Msa1361230:intron 30.0%
! TTAAGTTGTATGATGTTTGG+TGG + tig0017392:54132-54151 Msa1361230:intron 30.0%
TAGAATAATCAATCATGCCT+AGG - tig0017392:54409-54428 None:intergenic 30.0%
!! CTAGCATACAAACTTTGTTT+TGG + tig0017392:54426-54445 Msa1361230:three_prime_UTR 30.0%
AAAATCAAGTGTAGGACAAA+CGG - tig0017392:54576-54595 None:intergenic 30.0%
GGAGATATTTGCCTTAGTTT+GGG - tig0017392:53355-53374 None:intergenic 35.0%
AGAAAATAATGGCATGTTGG+GGG - tig0017392:53441-53460 None:intergenic 35.0%
GAGAAAATAATGGCATGTTG+GGG - tig0017392:53442-53461 None:intergenic 35.0%
ATGCATCATGTCCTCTTAAT+TGG - tig0017392:53515-53534 None:intergenic 35.0%
TACAGTTATAAAGAAGCCGA+GGG + tig0017392:53811-53830 Msa1361230:intron 35.0%
CAGAAGAAATAGAGAGATTC+TGG + tig0017392:54281-54300 Msa1361230:CDS 35.0%
! TCTTCACTTCTTTCTTTTGG+CGG - tig0017392:54332-54351 None:intergenic 35.0%
CAATTATATCTCTCTCTCCT+AGG + tig0017392:54389-54408 Msa1361230:three_prime_UTR 35.0%
TTTGTATTGCATCTCGTTGA+AGG + tig0017392:54463-54482 Msa1361230:three_prime_UTR 35.0%
! ATAAAGCTTGCTTCAGACAT+GGG - tig0017392:54498-54517 None:intergenic 35.0%
! TATAAAGCTTGCTTCAGACA+TGG - tig0017392:54499-54518 None:intergenic 35.0%
! TGTCTGAAGCAAGCTTTATA+TGG + tig0017392:54499-54518 Msa1361230:three_prime_UTR 35.0%
GGAGATATTTGCCTTAGTTT+GGG - tig0017392:53355-53374 None:intergenic 35.0%
AGAAAATAATGGCATGTTGG+GGG - tig0017392:53441-53460 None:intergenic 35.0%
GAGAAAATAATGGCATGTTG+GGG - tig0017392:53442-53461 None:intergenic 35.0%
ATGCATCATGTCCTCTTAAT+TGG - tig0017392:53515-53534 None:intergenic 35.0%
TACAGTTATAAAGAAGCCGA+GGG + tig0017392:53811-53830 Msa1361230:intron 35.0%
CAGAAGAAATAGAGAGATTC+TGG + tig0017392:54281-54300 Msa1361230:CDS 35.0%
! TCTTCACTTCTTTCTTTTGG+CGG - tig0017392:54332-54351 None:intergenic 35.0%
CAATTATATCTCTCTCTCCT+AGG + tig0017392:54389-54408 Msa1361230:three_prime_UTR 35.0%
TTTGTATTGCATCTCGTTGA+AGG + tig0017392:54463-54482 Msa1361230:three_prime_UTR 35.0%
! ATAAAGCTTGCTTCAGACAT+GGG - tig0017392:54498-54517 None:intergenic 35.0%
! TATAAAGCTTGCTTCAGACA+TGG - tig0017392:54499-54518 None:intergenic 35.0%
! TGTCTGAAGCAAGCTTTATA+TGG + tig0017392:54499-54518 Msa1361230:three_prime_UTR 35.0%
GGGAGATATTTGCCTTAGTT+TGG - tig0017392:53356-53375 None:intergenic 40.0%
GGTAGATTAGAAACCACCTT+CGG - tig0017392:53397-53416 None:intergenic 40.0%
CTGTATTCCTTTGACGTTTG+AGG + tig0017392:53721-53740 Msa1361230:intron 40.0%
CTACAGTTATAAAGAAGCCG+AGG + tig0017392:53810-53829 Msa1361230:intron 40.0%
ATTTCTTCTGCTTTACGACC+AGG - tig0017392:54271-54290 None:intergenic 40.0%
! GTCCTACACTTGATTTTCCT+TGG + tig0017392:54579-54598 Msa1361230:three_prime_UTR 40.0%
GGGAGATATTTGCCTTAGTT+TGG - tig0017392:53356-53375 None:intergenic 40.0%
GGTAGATTAGAAACCACCTT+CGG - tig0017392:53397-53416 None:intergenic 40.0%
CTGTATTCCTTTGACGTTTG+AGG + tig0017392:53721-53740 Msa1361230:intron 40.0%
CTACAGTTATAAAGAAGCCG+AGG + tig0017392:53810-53829 Msa1361230:intron 40.0%
ATTTCTTCTGCTTTACGACC+AGG - tig0017392:54271-54290 None:intergenic 40.0%
GGAAGAACGTTCCCAAACTA+AGG + tig0017392:53341-53360 Msa1361230:CDS 45.0%
!! GGAGGTTTGACTTGTGTTTC+GGG - tig0017392:53376-53395 None:intergenic 45.0%
!! CGGAGGTTTGACTTGTGTTT+CGG - tig0017392:53377-53396 None:intergenic 45.0%
AACACAAGTCAAACCTCCGA+AGG + tig0017392:53378-53397 Msa1361230:CDS 45.0%
AGATTAGAAACCACCTTCGG+AGG - tig0017392:53394-53413 None:intergenic 45.0%
AATAATGGCATGTTGGGGGT+GGG - tig0017392:53437-53456 None:intergenic 45.0%
AAATAATGGCATGTTGGGGG+TGG - tig0017392:53438-53457 None:intergenic 45.0%
TGTACGACCTAGTTGGAGAT+AGG + tig0017392:53673-53692 Msa1361230:CDS 45.0%
!! CCGTGATTTTGAGAAACCCT+CGG - tig0017392:53830-53849 None:intergenic 45.0%
CCGAGGGTTTCTCAAAATCA+CGG + tig0017392:53827-53846 Msa1361230:intron 45.0%
TAGAAACTACACAACGCAGC+AGG + tig0017392:54221-54240 Msa1361230:intron 45.0%
! AGCAGGTGGAATTTGATAGC+AGG + tig0017392:54238-54257 Msa1361230:intron 45.0%
GGAAGAACGTTCCCAAACTA+AGG + tig0017392:53341-53360 Msa1361230:CDS 45.0%
!! GGAGGTTTGACTTGTGTTTC+GGG - tig0017392:53376-53395 None:intergenic 45.0%
!! CGGAGGTTTGACTTGTGTTT+CGG - tig0017392:53377-53396 None:intergenic 45.0%
AACACAAGTCAAACCTCCGA+AGG + tig0017392:53378-53397 Msa1361230:CDS 45.0%
AGATTAGAAACCACCTTCGG+AGG - tig0017392:53394-53413 None:intergenic 45.0%
AATAATGGCATGTTGGGGGT+GGG - tig0017392:53437-53456 None:intergenic 45.0%
AAATAATGGCATGTTGGGGG+TGG - tig0017392:53438-53457 None:intergenic 45.0%
TGTACGACCTAGTTGGAGAT+AGG + tig0017392:53673-53692 Msa1361230:CDS 45.0%
!! CCGTGATTTTGAGAAACCCT+CGG - tig0017392:53830-53849 None:intergenic 45.0%
CCGAGGGTTTCTCAAAATCA+CGG + tig0017392:53827-53846 Msa1361230:intron 45.0%
TAGAAACTACACAACGCAGC+AGG + tig0017392:54221-54240 Msa1361230:intron 45.0%
! AGCAGGTGGAATTTGATAGC+AGG + tig0017392:54238-54257 Msa1361230:intron 45.0%
ACAAGTCAAACCTCCGAAGG+TGG + tig0017392:53381-53400 Msa1361230:intron 50.0%
! AGAAGTGAGCAGCACTGAGT+GGG + tig0017392:53605-53624 Msa1361230:CDS 50.0%
CATAGAGATGAGCGAGCAAG+AGG + tig0017392:53632-53651 Msa1361230:CDS 50.0%
AAGAGGAGGATCTCATTCGC+AGG + tig0017392:53649-53668 Msa1361230:CDS 50.0%
AAACTACACAACGCAGCAGG+TGG + tig0017392:54224-54243 Msa1361230:intron 50.0%
! TTGATAGCAGGTCGCATTCC+TGG + tig0017392:54250-54269 Msa1361230:CDS 50.0%
ACAAGTCAAACCTCCGAAGG+TGG + tig0017392:53381-53400 Msa1361230:intron 50.0%
! AGAAGTGAGCAGCACTGAGT+GGG + tig0017392:53605-53624 Msa1361230:CDS 50.0%
CATAGAGATGAGCGAGCAAG+AGG + tig0017392:53632-53651 Msa1361230:CDS 50.0%
AAGAGGAGGATCTCATTCGC+AGG + tig0017392:53649-53668 Msa1361230:CDS 50.0%
AAACTACACAACGCAGCAGG+TGG + tig0017392:54224-54243 Msa1361230:intron 50.0%
! TTGATAGCAGGTCGCATTCC+TGG + tig0017392:54250-54269 Msa1361230:CDS 50.0%
TGGGTGTGTGTGTGTGTTGG+GGG - tig0017392:53418-53437 None:intergenic 55.0%
GTGGGTGTGTGTGTGTGTTG+GGG - tig0017392:53419-53438 None:intergenic 55.0%
GGTGGGTGTGTGTGTGTGTT+GGG - tig0017392:53420-53439 None:intergenic 55.0%
! CAGAAGTGAGCAGCACTGAG+TGG + tig0017392:53604-53623 Msa1361230:CDS 55.0%
AGAGATGAGCGAGCAAGAGG+AGG + tig0017392:53635-53654 Msa1361230:CDS 55.0%
CGCAGGATGTACGACCTAGT+TGG + tig0017392:53666-53685 Msa1361230:CDS 55.0%
TGGGTGTGTGTGTGTGTTGG+GGG - tig0017392:53418-53437 None:intergenic 55.0%
GTGGGTGTGTGTGTGTGTTG+GGG - tig0017392:53419-53438 None:intergenic 55.0%
GGTGGGTGTGTGTGTGTGTT+GGG - tig0017392:53420-53439 None:intergenic 55.0%
! CAGAAGTGAGCAGCACTGAG+TGG + tig0017392:53604-53623 Msa1361230:CDS 55.0%
AGAGATGAGCGAGCAAGAGG+AGG + tig0017392:53635-53654 Msa1361230:CDS 55.0%
CGCAGGATGTACGACCTAGT+TGG + tig0017392:53666-53685 Msa1361230:CDS 55.0%
GGGTGGGTGTGTGTGTGTGT+TGG - tig0017392:53421-53440 None:intergenic 60.0%
GGGTGGGTGTGTGTGTGTGT+TGG - tig0017392:53421-53440 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0017392 gene 53327 54599 53327 ID=Msa1361230;Name=Msa1361230
tig0017392 mRNA 53327 54599 53327 ID=Msa1361230-mRNA-1;Parent=Msa1361230;Name=Msa1361230-mRNA-1;_AED=0.00;_eAED=0.00;_QI=12|0|0.33|1|0|0|3|251|88
tig0017392 exon 53327 53399 53327 ID=Msa1361230-mRNA-1:exon:12308;Parent=Msa1361230-mRNA-1
tig0017392 exon 53598 53694 53598 ID=Msa1361230-mRNA-1:exon:12309;Parent=Msa1361230-mRNA-1
tig0017392 exon 54240 54599 54240 ID=Msa1361230-mRNA-1:exon:12310;Parent=Msa1361230-mRNA-1
tig0017392 five_prime_UTR 53327 53338 53327 ID=Msa1361230-mRNA-1:five_prime_utr;Parent=Msa1361230-mRNA-1
tig0017392 CDS 53339 53399 53339 ID=Msa1361230-mRNA-1:cds;Parent=Msa1361230-mRNA-1
tig0017392 CDS 53598 53694 53598 ID=Msa1361230-mRNA-1:cds;Parent=Msa1361230-mRNA-1
tig0017392 CDS 54240 54348 54240 ID=Msa1361230-mRNA-1:cds;Parent=Msa1361230-mRNA-1
tig0017392 three_prime_UTR 54349 54599 54349 ID=Msa1361230-mRNA-1:three_prime_utr;Parent=Msa1361230-mRNA-1
Gene Sequence

>Msa1361230

ATGGAAGAACGTTCCCAAACTAAGGCAAATATCTCCCGAAACACAAGTCAAACCTCCGAAGCTGATGCAGAAGTGAGCAGCACTGAGTGGGAGTTCATAGAGATGAGCGAGCAAGAGGAGGATCTCATTCGCAGGATGTACGACCTAGTTGGAGATAGCAGGTGGAATTTGATAGCAGGTCGCATTCCTGGTCGTAAAGCAGAAGAAATAGAGAGATTCTGGATTATGAAACACGCTGATGCTTTTTCCGCCAAAAGAAAGAAGTGA

Protein sequence

>Msa1361230

MEERSQTKANISRNTSQTSEADAEVSSTEWEFIEMSEQEEDLIRRMYDLVGDSRWNLIAGRIPGRKAEEIERFWIMKHADAFSAKRKK*