AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1363840

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1363840 MtrunA17_Chr8g0387941 94.703 472 5 2 1 471 1 453 0.0 902
Msa1363840 MtrunA17_Chr4g0027241 47.312 465 220 3 6 465 15 459 1.20e-149 434
Msa1363840 MtrunA17_Chr1g0198081 44.469 461 236 2 8 468 41 481 5.50e-135 398
Msa1363840 MtrunA17_Chr1g0184721 30.786 471 286 7 1 465 1 437 5.25e-70 229
Msa1363840 MtrunA17_Chr6g0458931 26.834 477 300 11 5 466 13 455 3.25e-52 182
Msa1363840 MtrunA17_Chr4g0027261 44.792 192 98 3 274 465 1 184 9.24e-51 171
Msa1363840 MtrunA17_Chr2g0331721 26.539 471 305 10 2 465 24 460 1.01e-48 173
Msa1363840 MtrunA17_Chr7g0230011 27.578 446 282 10 28 466 32 443 6.35e-44 160
Msa1363840 MtrunA17_Chr6g0458921 27.803 446 282 9 28 466 16 428 1.02e-43 159
Msa1363840 MtrunA17_Chr6g0459031 26.068 468 297 10 6 466 3 428 4.20e-43 157
Msa1363840 MtrunA17_Chr8g0376991 25.216 464 298 10 14 466 11 436 8.89e-42 154
Msa1363840 MtrunA17_Chr2g0328081 26.282 468 296 10 6 466 3 428 8.08e-40 148
Msa1363840 MtrunA17_Chr6g0459021 25.427 468 308 11 6 466 14 447 5.15e-39 146
Msa1363840 MtrunA17_Chr6g0459001 26.804 485 281 12 26 466 22 476 7.78e-39 147
Msa1363840 MtrunA17_Chr4g0058161 26.582 474 300 14 3 465 11 447 8.18e-39 146
Msa1363840 MtrunA17_Chr3g0122921 25.214 468 291 11 6 466 13 428 1.83e-38 144
Msa1363840 MtrunA17_Chr2g0281021 87.671 73 9 0 399 471 1 73 2.36e-38 134
Msa1363840 MtrunA17_Chr5g0436281 27.845 413 258 10 57 465 4 380 2.93e-38 143
Msa1363840 MtrunA17_Chr3g0112971 28.238 386 232 11 88 465 85 433 4.16e-38 144
Msa1363840 MtrunA17_Chr1g0198531 27.801 482 275 16 4 465 8 436 1.41e-37 142
Msa1363840 MtrunA17_Chr6g0459011 27.765 425 268 9 42 460 1 392 2.42e-36 138
Msa1363840 MtrunA17_Chr4g0057851 26.018 442 280 10 33 464 17 421 3.29e-36 138
Msa1363840 MtrunA17_Chr3g0077651 25.885 452 286 12 28 466 54 469 1.34e-35 137
Msa1363840 MtrunA17_Chr3g0143701 25.858 437 279 12 15 436 73 479 2.23e-35 137
Msa1363840 MtrunA17_Chr3g0130641 27.083 480 300 18 13 468 23 476 5.05e-33 130
Msa1363840 MtrunA17_Chr5g0422741 26.667 480 283 15 5 465 9 438 5.57e-33 129
Msa1363840 MtrunA17_Chr1g0206871 24.742 485 296 13 25 468 14 470 1.23e-32 129
Msa1363840 MtrunA17_Chr1g0198541 27.093 454 272 14 28 465 26 436 1.63e-32 128
Msa1363840 MtrunA17_Chr3g0125081 27.216 485 302 17 8 467 19 477 1.63e-31 126
Msa1363840 MtrunA17_Chr3g0130651 27.416 445 270 15 15 436 20 434 3.96e-31 125
Msa1363840 MtrunA17_Chr1g0159761 25.991 454 275 15 17 439 21 444 2.39e-29 120
Msa1363840 MtrunA17_Chr3g0125101 26.884 491 297 17 8 467 19 478 2.44e-29 120
Msa1363840 MtrunA17_Chr4g0038711 24.947 473 317 13 5 463 52 500 1.30e-28 118
Msa1363840 MtrunA17_Chr3g0140761 24.795 488 304 17 14 468 24 481 7.98e-28 115
Msa1363840 MtrunA17_Chr5g0406351 24.318 440 276 13 15 433 96 499 4.59e-27 114
Msa1363840 MtrunA17_Chr3g0125091 25.866 491 302 17 8 467 19 478 6.51e-27 113
Msa1363840 MtrunA17_Chr4g0037201 24.795 488 302 17 17 468 53 511 9.87e-27 112
Msa1363840 MtrunA17_Chr8g0384661 24.895 474 314 15 15 468 42 493 1.57e-26 112
Msa1363840 MtrunA17_Chr1g0146491 24.194 496 309 17 9 468 7 471 2.29e-26 111
Msa1363840 MtrunA17_Chr2g0281341 25.636 472 294 16 15 466 84 518 1.90e-25 109
Msa1363840 MtrunA17_Chr1g0146391 23.614 487 307 14 11 466 2 454 2.09e-25 108
Msa1363840 MtrunA17_Chr5g0406331 24.524 473 303 15 15 468 75 512 3.93e-25 108
Msa1363840 MtrunA17_Chr1g0146411 25.116 430 284 12 17 430 27 434 2.11e-24 105
Msa1363840 MtrunA17_Chr3g0140541 26.411 443 267 14 17 430 19 431 3.28e-23 102
Msa1363840 MtrunA17_Chr5g0447221 26.185 443 284 13 12 432 15 436 7.65e-23 100
Msa1363840 MtrunA17_Chr1g0146331 21.875 480 323 15 11 465 10 462 1.74e-21 97.1
Msa1363840 MtrunA17_Chr3g0103231 25.000 468 289 16 17 452 17 454 2.43e-20 93.6
Msa1363840 MtrunA17_Chr3g0103211 26.395 466 285 18 17 452 17 454 1.64e-19 90.9
Msa1363840 MtrunA17_Chr1g0206851 24.843 318 205 10 172 466 72 378 1.77e-19 90.1
Msa1363840 MtrunA17_Chr1g0206831 26.460 291 181 10 172 440 35 314 2.83e-19 89.0
Msa1363840 MtrunA17_Chr3g0103221 24.384 406 269 11 37 422 41 428 4.24e-19 89.7
Msa1363840 MtrunA17_Chr3g0140531 22.222 486 321 11 9 465 10 467 9.34e-19 88.6
Msa1363840 MtrunA17_Chr2g0293331 22.292 480 314 17 15 465 4 453 2.19e-18 87.4
Msa1363840 MtrunA17_Chr5g0447231 23.349 424 266 11 25 417 24 419 2.63e-17 84.3
Msa1363840 MtrunA17_Chr3g0103271 24.709 429 266 14 19 417 20 421 2.73e-17 84.0
Msa1363840 MtrunA17_Chr1g0206821 28.571 224 137 7 262 468 313 530 7.23e-15 77.0
Msa1363840 MtrunA17_Chr3g0130661 30.601 183 109 6 296 466 2 178 7.82e-13 67.0
Msa1363840 MtrunA17_Chr7g0241841 25.152 493 292 23 3 465 16 461 1.40e-12 69.7
Msa1363840 MtrunA17_Chr4g0006031 24.524 473 294 18 14 465 31 461 1.09e-11 66.6
Msa1363840 MtrunA17_Chr4g0037361 23.790 496 286 22 7 467 21 459 3.84e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1363840 AT5G41800.1 76.008 471 94 1 1 471 1 452 0.0 733
Msa1363840 AT1G08230.2 43.709 453 235 2 14 465 15 448 5.93e-135 397
Msa1363840 AT1G08230.3 43.953 339 189 1 128 465 10 348 4.05e-99 301
Msa1363840 AT1G08230.4 43.902 328 183 1 139 465 2 329 2.29e-97 296
Msa1363840 AT1G08230.6 43.902 328 183 1 139 465 2 329 2.29e-97 296
Msa1363840 AT1G08230.5 43.902 328 183 1 139 465 2 329 2.29e-97 296
Msa1363840 AT5G40780.1 26.458 480 294 12 9 466 4 446 1.73e-47 170
Msa1363840 AT1G48640.1 25.887 479 302 12 4 466 12 453 2.10e-42 156
Msa1363840 AT2G39890.2 27.902 448 274 11 27 465 32 439 5.95e-42 155
Msa1363840 AT2G39890.1 27.902 448 274 11 27 465 32 439 5.95e-42 155
Msa1363840 AT2G39890.3 28.571 413 255 9 57 465 4 380 1.38e-40 150
Msa1363840 AT5G40780.3 26.667 435 272 11 42 466 1 398 2.57e-40 149
Msa1363840 AT5G40780.2 26.667 435 272 11 42 466 1 398 2.57e-40 149
Msa1363840 AT1G24400.1 26.190 462 296 11 14 466 16 441 3.85e-40 150
Msa1363840 AT3G55740.1 27.455 448 276 11 27 465 29 436 1.64e-39 148
Msa1363840 AT2G36590.1 27.902 448 274 11 27 465 26 433 6.73e-39 146
Msa1363840 AT1G67640.1 26.439 469 303 11 5 466 8 441 9.05e-39 146
Msa1363840 AT3G55740.2 27.845 413 258 9 57 465 4 380 2.65e-38 144
Msa1363840 AT3G55740.3 27.845 413 258 9 57 465 4 380 2.65e-38 144
Msa1363840 AT1G61270.1 25.688 436 277 12 7 433 18 415 3.69e-34 134
Msa1363840 AT1G71680.1 25.696 467 306 11 7 466 16 448 7.06e-34 132
Msa1363840 AT3G01760.1 24.161 447 292 10 1 433 1 414 3.64e-31 125
Msa1363840 AT1G25530.1 27.578 446 278 13 29 465 30 439 8.31e-31 124
Msa1363840 AT1G44100.1 25.000 496 305 18 10 468 14 479 1.42e-28 118
Msa1363840 AT1G10010.1 25.054 467 290 13 3 438 6 443 1.74e-27 115
Msa1363840 AT1G71680.2 26.316 380 241 12 7 380 16 362 1.79e-27 113
Msa1363840 AT1G10010.2 25.054 467 290 13 3 438 7 444 2.05e-27 115
Msa1363840 AT5G09220.1 24.638 483 302 16 17 467 39 491 1.74e-26 112
Msa1363840 AT1G77380.1 25.541 462 288 16 9 436 3 442 8.97e-26 110
Msa1363840 AT5G49630.1 24.740 481 308 16 15 467 26 480 3.82e-25 108
Msa1363840 AT2G36590.2 27.532 316 187 8 27 336 26 305 1.08e-24 104
Msa1363840 AT5G63850.1 23.188 483 309 16 17 467 12 464 2.68e-24 105
Msa1363840 AT1G58360.1 23.228 508 312 15 8 467 5 482 1.60e-23 103
Msa1363840 AT1G47670.1 23.830 470 290 13 15 455 82 512 4.45e-22 99.4
Msa1363840 AT5G23810.1 25.845 414 268 12 24 414 23 420 1.27e-16 82.4
Msa1363840 AT4G35180.1 23.060 451 288 14 28 462 59 466 6.03e-16 80.5
Msa1363840 AT2G38120.1 24.949 489 286 22 6 465 25 461 1.06e-11 67.4
Msa1363840 AT4G35180.2 24.468 282 184 8 28 303 59 317 3.03e-11 65.1

Find 155 sgRNAs with CRISPR-Local

Find 310 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTACTCTGCTGCAGTTTC+TGG 0.055926 tig0018089:-42483 Msa1363840:CDS
CAAAATCATCATTGACTTAT+TGG 0.111694 tig0018089:-41826 Msa1363840:CDS
GTTTCTGGGTATTGGGTATT+TGG 0.176881 tig0018089:-42468 Msa1363840:CDS
AACAATATAGATTTAATTCT+TGG 0.191163 tig0018089:+41663 None:intergenic
CACTATCAAACAGCTTAAAT+TGG 0.193422 tig0018089:+41717 None:intergenic
CAATAAGTCAATGATGATTT+TGG 0.212437 tig0018089:+41827 None:intergenic
TTTATCATCTTTCCAGTTGC+AGG 0.255166 tig0018089:+42543 None:intergenic
CTGCTGCAGTTTCTGGGTAT+TGG 0.260317 tig0018089:-42476 Msa1363840:CDS
TGTAGCCCAAGCTGAGAAAA+AGG 0.265214 tig0018089:+42927 None:intergenic
AGTGTACTATGTCTATTATT+TGG 0.273537 tig0018089:+45598 None:intergenic
TGCTGCAGTTTCTGGGTATT+GGG 0.278167 tig0018089:-42475 Msa1363840:CDS
GCCAGATGATGGACCTTCTT+TGG 0.282256 tig0018089:-42409 Msa1363840:CDS
TTGGCTCCAACTTGGGTCCT+TGG 0.286614 tig0018089:-42390 Msa1363840:CDS
CAAGTGCTATGTTTGGTTAA+AGG 0.289633 tig0018089:-41582 Msa1363840:three_prime_UTR
GCGAGGAAGAAGATTTCTTT+TGG 0.301221 tig0018089:+42003 None:intergenic
TGCTGTTGGTTTCATTCCTC+TGG 0.308064 tig0018089:-41894 Msa1363840:CDS
GCAACTGGAAAGATGATAAA+AGG 0.310014 tig0018089:-42540 Msa1363840:CDS
CCGTACGCATTCAGAGGATT+AGG 0.313937 tig0018089:-45236 Msa1363840:CDS
TCAAGTTGCTTATGAGATAA+TGG 0.316818 tig0018089:-42062 Msa1363840:CDS
CCACGCGTTAGGCAATTCTA+TGG 0.325920 tig0018089:-45622 Msa1363840:five_prime_UTR
AGTTTGTAAGATGGGGAGAT+AGG 0.337681 tig0018089:-41612 Msa1363840:three_prime_UTR
ACCTTCTTTGGCTCCAACTT+GGG 0.338327 tig0018089:-42397 Msa1363840:CDS
ATCAAGAACTAATTTCCTTA+TGG 0.340524 tig0018089:+41745 None:intergenic
GGCTACACCTTCCTTGTAGT+TGG 0.342809 tig0018089:-42911 Msa1363840:CDS
TGCTCCATGTGGTGCAATAT+TGG 0.345478 tig0018089:+43048 None:intergenic
CGAGTTCTCGGAAACGGATA+TGG 0.345658 tig0018089:+45108 None:intergenic
GAACTCGCCGCCGATGTCTT+AGG 0.352198 tig0018089:-45092 Msa1363840:CDS
AAAATTCTCAAACATGCCTT+TGG 0.360160 tig0018089:+41543 None:intergenic
TCTACTCTGCTGCAGTTTCT+GGG 0.376927 tig0018089:-42482 Msa1363840:CDS
CATTTAACAACGGCGATTGT+AGG 0.384219 tig0018089:-45278 Msa1363840:CDS
GACCTTCTTTGGCTCCAACT+TGG 0.385739 tig0018089:-42398 Msa1363840:CDS
TTTGTGTTCAGTTGTCTCTC+TGG 0.386553 tig0018089:+45646 None:intergenic
CGCCGGTATTGATTGCCGTT+TGG 0.387244 tig0018089:+44426 None:intergenic
AATTGCTGCAATTATGGAAA+TGG 0.392812 tig0018089:+42700 None:intergenic
CATGAGGCAGTAAGAGTAGA+AGG 0.394794 tig0018089:+45169 None:intergenic
GTAGGGGCAATCCTGCTTGC+TGG 0.394857 tig0018089:-44398 Msa1363840:CDS
CTTCTTCAACTCTTCGCAAT+CGG 0.397741 tig0018089:-42348 Msa1363840:CDS
CTCCTAAGTTCTCTCTTGGT+TGG 0.401737 tig0018089:-45549 Msa1363840:five_prime_UTR
TTCTCTCCTAAGTTCTCTCT+TGG 0.402896 tig0018089:-45553 Msa1363840:five_prime_UTR
TGGTGATTCTGATTCTCCTT+AGG 0.405695 tig0018089:+45496 None:intergenic
TGTAAAGTGTTCGAAGAATC+AGG 0.407219 tig0018089:+41981 None:intergenic
TATGTGTTATGCCGTAATTT+TGG 0.409726 tig0018089:-42511 Msa1363840:CDS
TTGGATCAACATTTCAATCA+TGG 0.409998 tig0018089:-41807 Msa1363840:CDS
ACTCTAGCACCTCCTGCAAC+TGG 0.420616 tig0018089:-42555 Msa1363840:CDS
CGGCGTCATCTTCATCTTGA+GGG 0.427559 tig0018089:+45473 None:intergenic
AGACATCGGCGGCGAGTTCT+CGG 0.429362 tig0018089:+45096 None:intergenic
ATGTTATGCCTAACAGTTAT+GGG 0.431198 tig0018089:-45200 Msa1363840:CDS
CGTACGCATTCAGAGGATTA+GGG 0.431714 tig0018089:-45235 Msa1363840:CDS
GTTATTTCAGTTTGTAAGAT+GGG 0.432603 tig0018089:-41620 Msa1363840:three_prime_UTR
CATCAAACTCTTCAAAATGT+TGG 0.435510 tig0018089:+42431 None:intergenic
GAAAAGTCAAGTGCTATGTT+TGG 0.438684 tig0018089:-41589 Msa1363840:three_prime_UTR
TCAATCATGGTGGCATTCAC+TGG 0.438826 tig0018089:-41794 Msa1363840:CDS
GTAGAATGTCACCAAAATTA+CGG 0.442251 tig0018089:+42500 None:intergenic
AAAATGGTTACTTGTATCTC+AGG 0.442563 tig0018089:+42659 None:intergenic
TGAGATAATGGAGAAGAAAT+CGG 0.444629 tig0018089:-42050 Msa1363840:CDS
ATCCAAACGGCAATCAATAC+CGG 0.447790 tig0018089:-44428 Msa1363840:CDS
CTCCTTAGGTGGGTTTGCCA+TGG 0.450574 tig0018089:+45510 None:intergenic
ACTCTTCAAAATGTTGGAGT+TGG 0.450754 tig0018089:+42437 None:intergenic
AAGACCCTTGAGGATTCCTT+AGG 0.451852 tig0018089:+42737 None:intergenic
AATGTTATGCCTAACAGTTA+TGG 0.453009 tig0018089:-45201 Msa1363840:CDS
GCCAAAGAAGGTCCATCATC+TGG 0.458042 tig0018089:+42408 None:intergenic
TCAATACCGGCGTTGGCGTA+GGG 0.464596 tig0018089:-44415 Msa1363840:CDS
TCAACTCTTCGCAATCGGTC+TGG 0.469711 tig0018089:-42343 Msa1363840:intron
GGACTATTCCTTGGAACCTA+AGG 0.470002 tig0018089:-42753 Msa1363840:CDS
ATTCACTGGTGCAGGAATCA+TGG 0.473389 tig0018089:-41780 Msa1363840:CDS
CTGGAGGACGAAAGCAGCTC+CGG 0.474259 tig0018089:+45453 None:intergenic
ATTTAACAACGGCGATTGTA+GGG 0.474479 tig0018089:-45277 Msa1363840:CDS
GGTTATTTCAGTTTGTAAGA+TGG 0.475308 tig0018089:-41621 Msa1363840:three_prime_UTR
TGATTCTGATTCTCCTTAGG+TGG 0.479279 tig0018089:+45499 None:intergenic
TTACGGCATAACACATAGTA+AGG 0.482389 tig0018089:+42517 None:intergenic
AAGAGTTTGATGCCAGATGA+TGG 0.482621 tig0018089:-42420 Msa1363840:CDS
GCTTGCTGGTCAATGCCTCC+AGG 0.485762 tig0018089:-44384 Msa1363840:intron
GATTCTGATTCTCCTTAGGT+GGG 0.485762 tig0018089:+45500 None:intergenic
CTTGATCATTGCGAAAATGC+CGG 0.487042 tig0018089:-45137 Msa1363840:CDS
TGGGTGCATTCTCGTCCATA+AGG 0.489029 tig0018089:-41760 Msa1363840:CDS
AATGGCGTGGTTGGTGCTGT+TGG 0.491300 tig0018089:-41908 Msa1363840:CDS
TTACAAGACCCATAACTGTT+AGG 0.492363 tig0018089:+45192 None:intergenic
AGTTGATGTGTCGTAGGGAA+TGG 0.492712 tig0018089:+42957 None:intergenic
CAAACTGAAATAACCTTGTG+TGG 0.494564 tig0018089:+41629 None:intergenic
ATGGTGGCATTCACTGGTGC+AGG 0.497413 tig0018089:-41788 Msa1363840:CDS
CCGGCGTCATCTTCATCTTG+AGG 0.503188 tig0018089:+45472 None:intergenic
TGAACACAAACCCACGCGTT+AGG 0.506374 tig0018089:-45633 Msa1363840:five_prime_UTR
CCCTCCAAGGGACTATTCCT+TGG 0.507686 tig0018089:-42762 Msa1363840:CDS
TGAGGATTCCTTAGGTTCCA+AGG 0.508139 tig0018089:+42745 None:intergenic
GTTTGTAAGATGGGGAGATA+GGG 0.509993 tig0018089:-41611 Msa1363840:three_prime_UTR
ATCAATACCGGCGTTGGCGT+AGG 0.512369 tig0018089:-44416 Msa1363840:CDS
AAAATTGCATGCCTTTGGAC+TGG 0.514853 tig0018089:+45434 None:intergenic
CATTGATGACAGGGTCGGGT+TGG 0.517341 tig0018089:-44472 Msa1363840:intron
GAAACGGATATGGCGGCGAC+CGG 0.520639 tig0018089:+45118 None:intergenic
TTCACTGGTGCAGGAATCAT+GGG 0.520800 tig0018089:-41779 Msa1363840:CDS
CATTCAGAGGATTAGGGTGG+GGG 0.524963 tig0018089:-45229 Msa1363840:CDS
TGTTGTATAAAATCATAGGC+AGG 0.525971 tig0018089:+41864 None:intergenic
GTGTCGTAGGGAATGGAAAG+TGG 0.532912 tig0018089:+42964 None:intergenic
GGTTCCAAGGAATAGTCCCT+TGG 0.544138 tig0018089:+42758 None:intergenic
TGTCGTAGGGAATGGAAAGT+GGG 0.547788 tig0018089:+42965 None:intergenic
TACGGCAGCGTTAGTATCGT+TGG 0.548155 tig0018089:+45254 None:intergenic
ACCCAAGTTGGAGCCAAAGA+AGG 0.550451 tig0018089:+42396 None:intergenic
TGCCATGGCAAACCCACCTA+AGG 0.551622 tig0018089:-45512 Msa1363840:exon
CGGCGGCGAGTTCTCGGAAA+CGG 0.551808 tig0018089:+45102 None:intergenic
GCGAGTCCAAGGACCCAAGT+TGG 0.554753 tig0018089:+42384 None:intergenic
GGAGACATCAATGGCGTGGT+TGG 0.554958 tig0018089:-41917 Msa1363840:CDS
GCATTCAGAGGATTAGGGTG+GGG 0.556854 tig0018089:-45230 Msa1363840:CDS
GTTGGTGCTTGTATTCATGC+AGG 0.557410 tig0018089:-42893 Msa1363840:intron
TCCAAGGAATAGTCCCTTGG+AGG 0.563185 tig0018089:+42761 None:intergenic
ACGGCAATCAATACCGGCGT+TGG 0.565368 tig0018089:-44422 Msa1363840:CDS
CGCATTCAGAGGATTAGGGT+GGG 0.565491 tig0018089:-45231 Msa1363840:CDS
ATCATCTTTCCAGTTGCAGG+AGG 0.565721 tig0018089:+42546 None:intergenic
GGAGGCATTGACCAGCAAGC+AGG 0.567407 tig0018089:+44387 None:intergenic
AAGTTCTCTCTTGGTTGGCG+TGG 0.569475 tig0018089:-45544 Msa1363840:five_prime_UTR
CAAAGACGATAGCGAGTCCA+AGG 0.572437 tig0018089:+42373 None:intergenic
AAATTCTTTAGTTTCTCCAA+AGG 0.577765 tig0018089:-41559 Msa1363840:three_prime_UTR
GAATACAAGCACCAACTACA+AGG 0.578514 tig0018089:+42900 None:intergenic
TTATTTCAGTTTGTAAGATG+GGG 0.580413 tig0018089:-41619 Msa1363840:three_prime_UTR
CATAGAATTGCCTAACGCGT+GGG 0.581260 tig0018089:+45623 None:intergenic
GCTGTTTGATAGTGAAGTTG+TGG 0.589328 tig0018089:-41708 Msa1363840:CDS
CCTAATCCTCTGAATGCGTA+CGG 0.589330 tig0018089:+45236 None:intergenic
CAATACCGGCGTTGGCGTAG+GGG 0.590022 tig0018089:-44414 Msa1363840:CDS
GATTACATCACCTAAGACAT+CGG 0.591318 tig0018089:+45082 None:intergenic
CCATAGAATTGCCTAACGCG+TGG 0.591931 tig0018089:+45622 None:intergenic
ACGCTGCCGTACGCATTCAG+AGG 0.593272 tig0018089:-45242 Msa1363840:CDS
AATTATGGAAATGGCAGTGA+AGG 0.596045 tig0018089:+42709 None:intergenic
CCAAGGAATAGTCCCTTGGA+GGG 0.596394 tig0018089:+42762 None:intergenic
AATTGATTGATATAGGTGAA+TGG 0.604073 tig0018089:-45316 Msa1363840:intron
TACTCCAATATTGCACCACA+TGG 0.605379 tig0018089:-43052 Msa1363840:CDS
AACACAAGTTGATGTGTCGT+AGG 0.607210 tig0018089:+42951 None:intergenic
GCTTTCGTCCTCCAGTCCAA+AGG 0.609297 tig0018089:-45445 Msa1363840:intron
ATTGCATGCCTTTGGACTGG+AGG 0.609574 tig0018089:+45437 None:intergenic
GATTGCCCCTACGCCAACGC+CGG 0.613136 tig0018089:+44409 None:intergenic
CACGCGTTAGGCAATTCTAT+GGG 0.613215 tig0018089:-45621 Msa1363840:five_prime_UTR
AAATCGGCAGATGTGAAGCA+AGG 0.618331 tig0018089:-42034 Msa1363840:CDS
ACACAAGTTGATGTGTCGTA+GGG 0.621227 tig0018089:+42952 None:intergenic
ACTGTACGACTTCATAGCAA+TGG 0.624920 tig0018089:-43020 Msa1363840:CDS
ACAAGCACCAACTACAAGGA+AGG 0.626871 tig0018089:+42904 None:intergenic
TGGAACCTAAGGAATCCTCA+AGG 0.628374 tig0018089:-42742 Msa1363840:CDS
TATAGGTGAATGGTGGCACG+CGG 0.632366 tig0018089:-45306 Msa1363840:intron
ACGCATTCAGAGGATTAGGG+TGG 0.633907 tig0018089:-45232 Msa1363840:CDS
GCGTGGCTAAACTCATGCCA+TGG 0.638366 tig0018089:-45527 Msa1363840:exon
CGTCATCTTCATCTTGAGGG+TGG 0.638459 tig0018089:+45476 None:intergenic
TAGGTGAATGGTGGCACGCG+GGG 0.647836 tig0018089:-45304 Msa1363840:CDS
ATAGGTGAATGGTGGCACGC+GGG 0.648320 tig0018089:-45305 Msa1363840:intron
GGAACCTAAGGAATCCTCAA+GGG 0.649619 tig0018089:-42741 Msa1363840:CDS
AATCGGCAGATGTGAAGCAA+GGG 0.649745 tig0018089:-42033 Msa1363840:CDS
GATCAACATTTCAATCATGG+TGG 0.650071 tig0018089:-41804 Msa1363840:CDS
CGCCAACCAAGAGAGAACTT+AGG 0.653575 tig0018089:+45547 None:intergenic
TACAGTTTCAATGCTCCATG+TGG 0.654464 tig0018089:+43037 None:intergenic
CAAGGAATAGTCCCTTGGAG+GGG 0.654932 tig0018089:+42763 None:intergenic
GATCAAGAACTTTAGACATG+AGG 0.658137 tig0018089:+45153 None:intergenic
GTTCTCGGAAACGGATATGG+CGG 0.683221 tig0018089:+45111 None:intergenic
CCTCAAGATGAAGATGACGC+CGG 0.694783 tig0018089:-45472 Msa1363840:CDS
GAAGGCACTGAAGACCCTTG+AGG 0.696373 tig0018089:+42727 None:intergenic
CGCGGGGTATCATTTAACAA+CGG 0.699944 tig0018089:-45288 Msa1363840:CDS
ATAGGCAGGACAAAATCCAG+AGG 0.715818 tig0018089:+41878 None:intergenic
TGATTGATATAGGTGAATGG+TGG 0.718250 tig0018089:-45313 Msa1363840:intron
TACATCACCTAAGACATCGG+CGG 0.726868 tig0018089:+45085 None:intergenic
AGTGTTCGAAGAATCAGGCG+AGG 0.728618 tig0018089:+41986 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAAAAAAAAAAAAAGATA+CGG - tig0018089:41514-41533 Msa1363840:three_prime_UTR 10.0%
!! TAATTATTAATGTTTGAAAA+TGG + tig0018089:42229-42248 None:intergenic 10.0%
!! ACTATTTATCTTATAAAATA+TGG + tig0018089:42492-42511 None:intergenic 10.0%
!! GTAAAATTACTAAAAATAAT+TGG + tig0018089:42859-42878 None:intergenic 10.0%
!! AAAAAAAAATTAATATTGCA+CGG - tig0018089:42911-42930 Msa1363840:CDS 10.0%
!! ATTAAGAACAAAAAAAAAAT+CGG + tig0018089:43985-44004 None:intergenic 10.0%
!!! ATTTAAGATTTTCTATATTC+AGG - tig0018089:42198-42217 Msa1363840:intron 15.0%
!!! ATCAATAATTGTTTTTTTAG+AGG - tig0018089:42310-42329 Msa1363840:intron 15.0%
!! AAATAATTTCCACAATTATT+TGG - tig0018089:42412-42431 Msa1363840:CDS 15.0%
!!! TGAATTTTCTTAAATTTTGT+TGG - tig0018089:42711-42730 Msa1363840:CDS 15.0%
!! TAAATAAATAAGGGTAAAAT+AGG - tig0018089:42937-42956 Msa1363840:CDS 15.0%
!! AAATAAATAAGGGTAAAATA+GGG - tig0018089:42938-42957 Msa1363840:CDS 15.0%
!!! TAAGAAAAGTGTATAAATAA+AGG - tig0018089:43197-43216 Msa1363840:intron 15.0%
!!! AAGAAAAGTGTATAAATAAA+GGG - tig0018089:43198-43217 Msa1363840:intron 15.0%
!! TATTTAGTGTTATATTGTAT+TGG - tig0018089:43262-43281 Msa1363840:intron 15.0%
!!! GACACTTTTTTTTAAAAAAT+GGG - tig0018089:43332-43351 Msa1363840:intron 15.0%
!! GACAAATATGTAAAATAAAT+AGG - tig0018089:43795-43814 Msa1363840:intron 15.0%
!! AAAATGTATTCTATAAAAAG+AGG - tig0018089:43860-43879 Msa1363840:intron 15.0%
!! TTGTTCTTAATTAGTAATAT+AGG - tig0018089:43994-44013 Msa1363840:intron 15.0%
!!! ATTTAGAATGTTAAAATACT+TGG - tig0018089:44070-44089 Msa1363840:intron 15.0%
!! TAATATTTCAATTTGTGATT+TGG - tig0018089:44115-44134 Msa1363840:intron 15.0%
!! AATTATTTCAATTTAGAAAG+AGG + tig0018089:44578-44597 None:intergenic 15.0%
!! TCATTATGAATTAATTGTAT+AGG - tig0018089:44629-44648 Msa1363840:intron 15.0%
!!! TTTAGTCTTATCATATTTAT+AGG - tig0018089:45117-45136 Msa1363840:CDS 15.0%
!! AACAATATAGATTTAATTCT+TGG + tig0018089:45549-45568 None:intergenic 15.0%
!! AGATATGAATTGATTGATAT+AGG - tig0018089:41886-41905 Msa1363840:CDS 20.0%
!!! TTCTTTTCTAATTCTTTTTC+TGG - tig0018089:42174-42193 Msa1363840:intron 20.0%
!!! GACTTTAATTTCAATTTGTA+CGG + tig0018089:42273-42292 None:intergenic 20.0%
!!! AAACAATTATTGATTGATAG+AGG + tig0018089:42304-42323 None:intergenic 20.0%
!! ATTAGATTACCAAATAATTG+TGG + tig0018089:42424-42443 None:intergenic 20.0%
!!! ATATCATTGATTCTAATCAT+TGG + tig0018089:42562-42581 None:intergenic 20.0%
!!! TTTTGTAATCTTTATCCAAA+CGG - tig0018089:42768-42787 Msa1363840:CDS 20.0%
!! AATAAATAAGGGTAAAATAG+GGG - tig0018089:42939-42958 Msa1363840:CDS 20.0%
!!! TCTAGTATTTTTCTTGTATA+TGG + tig0018089:43029-43048 None:intergenic 20.0%
!!! TTTTTTTTCTGCTTTTTTTG+AGG - tig0018089:43111-43130 Msa1363840:intron 20.0%
!!! TTTTTTTCTGCTTTTTTTGA+GGG - tig0018089:43112-43131 Msa1363840:intron 20.0%
!!! AGAAAAGTGTATAAATAAAG+GGG - tig0018089:43199-43218 Msa1363840:intron 20.0%
!! AAAGTGGAATATATTGTATT+GGG - tig0018089:43222-43241 Msa1363840:intron 20.0%
!! AGTTGTATTGAAAATTGAAA+TGG - tig0018089:43295-43314 Msa1363840:intron 20.0%
!!! GGACACTTTTTTTTAAAAAA+TGG - tig0018089:43331-43350 Msa1363840:intron 20.0%
!! ATTCAATACTAGTATGAAAT+AGG - tig0018089:43515-43534 Msa1363840:intron 20.0%
!! TATGAATTATGATAACTCTT+AGG - tig0018089:43600-43619 Msa1363840:intron 20.0%
!!! TATTTTAGAACCATTATTTG+TGG + tig0018089:43656-43675 None:intergenic 20.0%
!! TCAAGATAATTAAGAAAAAC+TGG - tig0018089:43714-43733 Msa1363840:intron 20.0%
!! TGTAACATAAATGTAATCAA+GGG + tig0018089:44377-44396 None:intergenic 20.0%
!! TTGTAACATAAATGTAATCA+AGG + tig0018089:44378-44397 None:intergenic 20.0%
!! ACAATTAATTCATAATGACA+TGG + tig0018089:44627-44646 None:intergenic 20.0%
!!! GTTTTGTTGTATAAAATCAT+AGG + tig0018089:45352-45371 None:intergenic 20.0%
! AGTGTACTATGTCTATTATT+TGG + tig0018089:41614-41633 None:intergenic 25.0%
! AATTGATTGATATAGGTGAA+TGG - tig0018089:41893-41912 Msa1363840:CDS 25.0%
!! TTTGTACGGTTTTTCTAATT+AGG + tig0018089:42259-42278 None:intergenic 25.0%
! CCATATCTTGTAATAAAACT+TGG + tig0018089:42585-42604 None:intergenic 25.0%
!!! CCAAGTTTTATTACAAGATA+TGG - tig0018089:42582-42601 Msa1363840:intron 25.0%
!!! AGTTTTATTACAAGATATGG+TGG - tig0018089:42585-42604 Msa1363840:intron 25.0%
! TGCACGGATTTAAATAAATA+AGG - tig0018089:42927-42946 Msa1363840:CDS 25.0%
! GCACGGATTTAAATAAATAA+GGG - tig0018089:42928-42947 Msa1363840:CDS 25.0%
!!! AAGTGGGGTATTATATTTTT+TGG + tig0018089:43059-43078 None:intergenic 25.0%
! GAAAGTGGAATATATTGTAT+TGG - tig0018089:43221-43240 Msa1363840:intron 25.0%
!! TTGAAATGGATCAGTTATTT+TGG - tig0018089:43309-43328 Msa1363840:intron 25.0%
!!! TGAAATGGATCAGTTATTTT+GGG - tig0018089:43310-43329 Msa1363840:intron 25.0%
!! TGATTTTCATTCAACATAGA+CGG - tig0018089:43401-43420 Msa1363840:intron 25.0%
!! TTTCATACTAGTATTGAATG+TGG + tig0018089:43514-43533 None:intergenic 25.0%
!! TATATGGAACTTTTCACATT+TGG - tig0018089:43558-43577 Msa1363840:intron 25.0%
! AGAGTTATCATAATTCATAC+AGG + tig0018089:43599-43618 None:intergenic 25.0%
! TATATTCTCTCCACAAATAA+TGG - tig0018089:43643-43662 Msa1363840:intron 25.0%
! TTAATTATCTTGATCTAGCA+TGG + tig0018089:43707-43726 None:intergenic 25.0%
!!! AGGCAAGAATTTTTTTCTAT+AGG - tig0018089:43880-43899 Msa1363840:intron 25.0%
! TAACATAAATGTAATCAAGG+GGG + tig0018089:44375-44394 None:intergenic 25.0%
! GTAACATAAATGTAATCAAG+GGG + tig0018089:44376-44395 None:intergenic 25.0%
! AATTTAGAAAGAGGACAAAA+TGG + tig0018089:44569-44588 None:intergenic 25.0%
! AATCTAGCAATATATTTGTC+TGG + tig0018089:44602-44621 None:intergenic 25.0%
! ACAAATATAAGGGATTGAAT+TGG - tig0018089:44902-44921 Msa1363840:intron 25.0%
! TAATTACAAGTTTGTAATCG+CGG + tig0018089:44940-44959 None:intergenic 25.0%
! TACTGTAATTGTTGATATTG+CGG - tig0018089:44960-44979 Msa1363840:intron 25.0%
!! CAATAAGTCAATGATGATTT+TGG + tig0018089:45385-45404 None:intergenic 25.0%
!!! CAAAATCATCATTGACTTAT+TGG - tig0018089:45383-45402 Msa1363840:intron 25.0%
! ATCAAGAACTAATTTCCTTA+TGG + tig0018089:45467-45486 None:intergenic 25.0%
! TTAATATGAAAAACCACACA+AGG - tig0018089:45567-45586 Msa1363840:five_prime_UTR 25.0%
!! GTTATTTCAGTTTGTAAGAT+GGG - tig0018089:45589-45608 Msa1363840:five_prime_UTR 25.0%
! TTATTTCAGTTTGTAAGATG+GGG - tig0018089:45590-45609 Msa1363840:five_prime_UTR 25.0%
!! AAATTCTTTAGTTTCTCCAA+AGG - tig0018089:45650-45669 Msa1363840:five_prime_UTR 25.0%
ATAGAAAAATTGCATGCCTT+TGG + tig0018089:41783-41802 None:intergenic 30.0%
AATGTTATGCCTAACAGTTA+TGG - tig0018089:42008-42027 Msa1363840:CDS 30.0%
ATGTTATGCCTAACAGTTAT+GGG - tig0018089:42009-42028 Msa1363840:CDS 30.0%
AACGCATATCAGATAATGAA+TGG + tig0018089:42359-42378 None:intergenic 30.0%
TGAGTAGTAAGTTAGTAACA+AGG - tig0018089:42649-42668 Msa1363840:intron 30.0%
ATCGTACAAATCTAGAAATG+CGG + tig0018089:42690-42709 None:intergenic 30.0%
AAATAATTGGAAAGCTAACC+TGG + tig0018089:42846-42865 None:intergenic 30.0%
AAAAAACTAGTTGCTCTCAT+TGG + tig0018089:43097-43116 None:intergenic 30.0%
TGTATAAATAAAGGGGAAAG+TGG - tig0018089:43206-43225 Msa1363840:intron 30.0%
AAAAAATGGGTCAGTTATTC+CGG - tig0018089:43345-43364 Msa1363840:intron 30.0%
! ATACTAGTATTGAATGTGGA+TGG + tig0018089:43510-43529 None:intergenic 30.0%
AAATACTTGGCTCAAGAAAA+TGG - tig0018089:44083-44102 Msa1363840:intron 30.0%
GCGATTAAACTTGATATTGT+AGG - tig0018089:44409-44428 Msa1363840:CDS 30.0%
AATTGCTGCAATTATGGAAA+TGG + tig0018089:44512-44531 None:intergenic 30.0%
! TCCATAATTGCAGCAATTTT+TGG - tig0018089:44514-44533 Msa1363840:intron 30.0%
! CCATAATTGCAGCAATTTTT+GGG - tig0018089:44515-44534 Msa1363840:intron 30.0%
AAAATGGTTACTTGTATCTC+AGG + tig0018089:44553-44572 None:intergenic 30.0%
! TATGTGTTATGCCGTAATTT+TGG - tig0018089:44698-44717 Msa1363840:intron 30.0%
GTAGAATGTCACCAAAATTA+CGG + tig0018089:44712-44731 None:intergenic 30.0%
CATCAAACTCTTCAAAATGT+TGG + tig0018089:44781-44800 None:intergenic 30.0%
AGCTCACAAACACAAATATA+AGG - tig0018089:44891-44910 Msa1363840:intron 30.0%
GCTCACAAACACAAATATAA+GGG - tig0018089:44892-44911 Msa1363840:intron 30.0%
!! GATTGAATTGGTTTGAACTA+TGG - tig0018089:44914-44933 Msa1363840:intron 30.0%
!! ATTGTAAAGGCTTTGGAAAT+TGG + tig0018089:45040-45059 None:intergenic 30.0%
! TCAAGTTGCTTATGAGATAA+TGG - tig0018089:45147-45166 Msa1363840:CDS 30.0%
!! TGAGATAATGGAGAAGAAAT+CGG - tig0018089:45159-45178 Msa1363840:CDS 30.0%
! ACACTTTACATGATTTTCTG+TGG - tig0018089:45241-45260 Msa1363840:CDS 30.0%
TGTTGTATAAAATCATAGGC+AGG + tig0018089:45348-45367 None:intergenic 30.0%
! TAAGTCAATGATGATTTTGG+AGG + tig0018089:45382-45401 None:intergenic 30.0%
TTGGATCAACATTTCAATCA+TGG - tig0018089:45402-45421 Msa1363840:intron 30.0%
CACTATCAAACAGCTTAAAT+TGG + tig0018089:45495-45514 None:intergenic 30.0%
! GGTTATTTCAGTTTGTAAGA+TGG - tig0018089:45588-45607 Msa1363840:five_prime_UTR 30.0%
AAAATTCTCAAACATGCCTT+TGG + tig0018089:45669-45688 None:intergenic 30.0%
TGATTGATATAGGTGAATGG+TGG - tig0018089:41896-41915 Msa1363840:CDS 35.0%
ATTTAACAACGGCGATTGTA+GGG - tig0018089:41932-41951 Msa1363840:CDS 35.0%
TTACAAGACCCATAACTGTT+AGG + tig0018089:42020-42039 None:intergenic 35.0%
GATCAAGAACTTTAGACATG+AGG + tig0018089:42059-42078 None:intergenic 35.0%
GATTACATCACCTAAGACAT+CGG + tig0018089:42130-42149 None:intergenic 35.0%
GAGAAGAAACACTCAACAAA+AGG + tig0018089:42528-42547 None:intergenic 35.0%
!! TTGTTGGTTTCATTGATGAC+AGG - tig0018089:42727-42746 Msa1363840:CDS 35.0%
!! TGTTGGTTTCATTGATGACA+GGG - tig0018089:42728-42747 Msa1363840:CDS 35.0%
CAAAGTAGGTCAATTATACC+GGG + tig0018089:43162-43181 None:intergenic 35.0%
CCAAAGTAGGTCAATTATAC+CGG + tig0018089:43163-43182 None:intergenic 35.0%
TCTGTGTGAAAAACCAAAGT+AGG + tig0018089:43176-43195 None:intergenic 35.0%
AAAAATGGGTCAGTTATTCC+GGG - tig0018089:43346-43365 Msa1363840:intron 35.0%
AGTGAGTGACAATCTGAAAA+CGG - tig0018089:43448-43467 Msa1363840:intron 35.0%
CATATACACCATAGTGATAG+GGG + tig0018089:43544-43563 None:intergenic 35.0%
CCATATACACCATAGTGATA+GGG + tig0018089:43545-43564 None:intergenic 35.0%
TCCATATACACCATAGTGAT+AGG + tig0018089:43546-43565 None:intergenic 35.0%
!!! TTTTATTTTGCTTCCGATCG+TGG - tig0018089:43930-43949 Msa1363840:intron 35.0%
AATTATGGAAATGGCAGTGA+AGG + tig0018089:44503-44522 None:intergenic 35.0%
CCCAAAAATTGCTGCAATTA+TGG + tig0018089:44518-44537 None:intergenic 35.0%
!! ATTGCAGCAATTTTTGGGAA+TGG - tig0018089:44520-44539 Msa1363840:intron 35.0%
TTTATCATCTTTCCAGTTGC+AGG + tig0018089:44669-44688 None:intergenic 35.0%
GCAACTGGAAAGATGATAAA+AGG - tig0018089:44669-44688 Msa1363840:intron 35.0%
TTACGGCATAACACATAGTA+AGG + tig0018089:44695-44714 None:intergenic 35.0%
ACTCTTCAAAATGTTGGAGT+TGG + tig0018089:44775-44794 None:intergenic 35.0%
! TGTAAAGTGTTCGAAGAATC+AGG + tig0018089:45231-45250 None:intergenic 35.0%
CCATTGATGTCTCCAAAAAA+CGG + tig0018089:45286-45305 None:intergenic 35.0%
GATCAACATTTCAATCATGG+TGG - tig0018089:45405-45424 Msa1363840:intron 35.0%
CAAACTGAAATAACCTTGTG+TGG + tig0018089:45583-45602 None:intergenic 35.0%
GAAAAGTCAAGTGCTATGTT+TGG - tig0018089:45620-45639 Msa1363840:five_prime_UTR 35.0%
CAAGTGCTATGTTTGGTTAA+AGG - tig0018089:45627-45646 Msa1363840:five_prime_UTR 35.0%
TTTGTGTTCAGTTGTCTCTC+TGG + tig0018089:41566-41585 None:intergenic 40.0%
TTCTCTCCTAAGTTCTCTCT+TGG - tig0018089:41656-41675 Msa1363840:three_prime_UTR 40.0%
GATTCTGATTCTCCTTAGGT+GGG + tig0018089:41712-41731 None:intergenic 40.0%
TGATTCTGATTCTCCTTAGG+TGG + tig0018089:41713-41732 None:intergenic 40.0%
TGGTGATTCTGATTCTCCTT+AGG + tig0018089:41716-41735 None:intergenic 40.0%
AAAATTGCATGCCTTTGGAC+TGG + tig0018089:41778-41797 None:intergenic 40.0%
CATTTAACAACGGCGATTGT+AGG - tig0018089:41931-41950 Msa1363840:CDS 40.0%
CTTGATCATTGCGAAAATGC+CGG - tig0018089:42072-42091 Msa1363840:CDS 40.0%
AGTGTTTCTTCTCTCTCGTA+AGG - tig0018089:42535-42554 Msa1363840:CDS 40.0%
ATCCAAACGGCAATCAATAC+CGG - tig0018089:42781-42800 Msa1363840:CDS 40.0%
TAATTGGAAAGCTAACCTGG+AGG + tig0018089:42843-42862 None:intergenic 40.0%
!! CTCATTGGTGTCACTATCAT+GGG + tig0018089:43082-43101 None:intergenic 40.0%
!! TCTCATTGGTGTCACTATCA+TGG + tig0018089:43083-43102 None:intergenic 40.0%
CCGGTATAATTGACCTACTT+TGG - tig0018089:43160-43179 Msa1363840:intron 40.0%
GTGAGTGACAATCTGAAAAC+GGG - tig0018089:43449-43468 Msa1363840:intron 40.0%
CCCTATCACTATGGTGTATA+TGG - tig0018089:43542-43561 Msa1363840:intron 40.0%
CACATTTGGTCCACTTTGTT+TGG - tig0018089:43572-43591 Msa1363840:intron 40.0%
TCATACAGGTCCAAACAAAG+TGG + tig0018089:43585-43604 None:intergenic 40.0%
! TGTCGGATCTTTTATGGAAC+AGG - tig0018089:43954-43973 Msa1363840:intron 40.0%
! GTCGGATCTTTTATGGAACA+GGG - tig0018089:43955-43974 Msa1363840:intron 40.0%
! TCGGATCTTTTATGGAACAG+GGG - tig0018089:43956-43975 Msa1363840:intron 40.0%
TACTCCAATATTGCACCACA+TGG - tig0018089:44157-44176 Msa1363840:intron 40.0%
TACAGTTTCAATGCTCCATG+TGG + tig0018089:44175-44194 None:intergenic 40.0%
ACTGTACGACTTCATAGCAA+TGG - tig0018089:44189-44208 Msa1363840:intron 40.0%
!! ACACAAGTTGATGTGTCGTA+GGG + tig0018089:44260-44279 None:intergenic 40.0%
!! AACACAAGTTGATGTGTCGT+AGG + tig0018089:44261-44280 None:intergenic 40.0%
! TGTTCCCTTTTTCTCAGCTT+GGG - tig0018089:44277-44296 Msa1363840:intron 40.0%
GAATACAAGCACCAACTACA+AGG + tig0018089:44312-44331 None:intergenic 40.0%
TGCTTGTATTCATGCAGGTA+TGG - tig0018089:44321-44340 Msa1363840:intron 40.0%
ATCAAAAAATGCCCCTCCAA+GGG - tig0018089:44435-44454 Msa1363840:CDS 40.0%
!! GTTTCTGGGTATTGGGTATT+TGG - tig0018089:44741-44760 Msa1363840:intron 40.0%
AAGAGTTTGATGCCAGATGA+TGG - tig0018089:44789-44808 Msa1363840:intron 40.0%
CTTCTTCAACTCTTCGCAAT+CGG - tig0018089:44861-44880 Msa1363840:intron 40.0%
GTTGATATTGCGGACAAATG+CGG - tig0018089:44970-44989 Msa1363840:intron 40.0%
GTAGTTGCTATAAGTCGTAG+AGG - tig0018089:45011-45030 Msa1363840:intron 40.0%
CCAAAGCCTTTACAATGTGA+CGG - tig0018089:45044-45063 Msa1363840:CDS 40.0%
CAAAGACCGTCACATTGTAA+AGG + tig0018089:45053-45072 None:intergenic 40.0%
GTCTTTGCAGGTTGCAATTT+CGG - tig0018089:45066-45085 Msa1363840:CDS 40.0%
! GCGAGGAAGAAGATTTCTTT+TGG + tig0018089:45209-45228 None:intergenic 40.0%
!!! CCGTTTTTTGGAGACATCAA+TGG - tig0018089:45283-45302 Msa1363840:CDS 40.0%
!! GCTGTTTGATAGTGAAGTTG+TGG - tig0018089:45501-45520 Msa1363840:CDS 40.0%
AGTTTGTAAGATGGGGAGAT+AGG - tig0018089:45597-45616 Msa1363840:five_prime_UTR 40.0%
GTTTGTAAGATGGGGAGATA+GGG - tig0018089:45598-45617 Msa1363840:five_prime_UTR 40.0%
CATAGAATTGCCTAACGCGT+GGG + tig0018089:41589-41608 None:intergenic 45.0%
CACGCGTTAGGCAATTCTAT+GGG - tig0018089:41588-41607 Msa1363840:three_prime_UTR 45.0%
CTCCTAAGTTCTCTCTTGGT+TGG - tig0018089:41660-41679 Msa1363840:three_prime_UTR 45.0%
! CGCGGGGTATCATTTAACAA+CGG - tig0018089:41921-41940 Msa1363840:CDS 45.0%
CCTAATCCTCTGAATGCGTA+CGG + tig0018089:41976-41995 None:intergenic 45.0%
CGTACGCATTCAGAGGATTA+GGG - tig0018089:41974-41993 Msa1363840:CDS 45.0%
CATGAGGCAGTAAGAGTAGA+AGG + tig0018089:42043-42062 None:intergenic 45.0%
TACATCACCTAAGACATCGG+CGG + tig0018089:42127-42146 None:intergenic 45.0%
! GGTTTCATTGATGACAGGGT+CGG - tig0018089:42732-42751 Msa1363840:CDS 45.0%
! GTTTCATTGATGACAGGGTC+GGG - tig0018089:42733-42752 Msa1363840:CDS 45.0%
TGTCACTATCATGGGAAGTG+GGG + tig0018089:43074-43093 None:intergenic 45.0%
! GTGTCACTATCATGGGAAGT+GGG + tig0018089:43075-43094 None:intergenic 45.0%
GTAGGTCAATTATACCGGGA+CGG + tig0018089:43158-43177 None:intergenic 45.0%
GACAATCTGAAAACGGGTGA+TGG - tig0018089:43455-43474 Msa1363840:intron 45.0%
ATAGGTGACCCCTATCACTA+TGG - tig0018089:43533-43552 Msa1363840:intron 45.0%
AGCATGGACAAGCTAACAAG+TGG + tig0018089:43691-43710 None:intergenic 45.0%
AAAGATCCGACATCCACGAT+CGG + tig0018089:43946-43965 None:intergenic 45.0%
! CGTGGATGTCGGATCTTTTA+TGG - tig0018089:43948-43967 Msa1363840:intron 45.0%
TGCTCCATGTGGTGCAATAT+TGG + tig0018089:44164-44183 None:intergenic 45.0%
TGTCGTAGGGAATGGAAAGT+GGG + tig0018089:44247-44266 None:intergenic 45.0%
! AGTTGATGTGTCGTAGGGAA+TGG + tig0018089:44255-44274 None:intergenic 45.0%
! GTGTTCCCTTTTTCTCAGCT+TGG - tig0018089:44276-44295 Msa1363840:intron 45.0%
GTAGCCCAAGCTGAGAAAAA+GGG + tig0018089:44284-44303 None:intergenic 45.0%
TGTAGCCCAAGCTGAGAAAA+AGG + tig0018089:44285-44304 None:intergenic 45.0%
ACAAGCACCAACTACAAGGA+AGG + tig0018089:44308-44327 None:intergenic 45.0%
! GTTGGTGCTTGTATTCATGC+AGG - tig0018089:44316-44335 Msa1363840:intron 45.0%
CATCAAAAAATGCCCCTCCA+AGG - tig0018089:44434-44453 Msa1363840:CDS 45.0%
GGACTATTCCTTGGAACCTA+AGG - tig0018089:44456-44475 Msa1363840:CDS 45.0%
TGAGGATTCCTTAGGTTCCA+AGG + tig0018089:44467-44486 None:intergenic 45.0%
TGGAACCTAAGGAATCCTCA+AGG - tig0018089:44467-44486 Msa1363840:intron 45.0%
GGAACCTAAGGAATCCTCAA+GGG - tig0018089:44468-44487 Msa1363840:intron 45.0%
AAGACCCTTGAGGATTCCTT+AGG + tig0018089:44475-44494 None:intergenic 45.0%
ATCATCTTTCCAGTTGCAGG+AGG + tig0018089:44666-44685 None:intergenic 45.0%
TTCTACTCTGCTGCAGTTTC+TGG - tig0018089:44726-44745 Msa1363840:intron 45.0%
TCTACTCTGCTGCAGTTTCT+GGG - tig0018089:44727-44746 Msa1363840:intron 45.0%
! TGCTGCAGTTTCTGGGTATT+GGG - tig0018089:44734-44753 Msa1363840:intron 45.0%
! ACCTTCTTTGGCTCCAACTT+GGG - tig0018089:44812-44831 Msa1363840:intron 45.0%
CCGTCACATTGTAAAGGCTT+TGG + tig0018089:45047-45066 None:intergenic 45.0%
TACAATGTGACGGTCTTTGC+AGG - tig0018089:45054-45073 Msa1363840:CDS 45.0%
AAATCGGCAGATGTGAAGCA+AGG - tig0018089:45175-45194 Msa1363840:CDS 45.0%
AATCGGCAGATGTGAAGCAA+GGG - tig0018089:45176-45195 Msa1363840:CDS 45.0%
!!! GCAGCTATGTTGCCGTTTTT+TGG - tig0018089:45271-45290 Msa1363840:CDS 45.0%
!! TTTTGGAGACATCAATGGCG+TGG - tig0018089:45288-45307 Msa1363840:CDS 45.0%
!! TGCTGTTGGTTTCATTCCTC+TGG - tig0018089:45315-45334 Msa1363840:intron 45.0%
ATAGGCAGGACAAAATCCAG+AGG + tig0018089:45334-45353 None:intergenic 45.0%
TCAATCATGGTGGCATTCAC+TGG - tig0018089:45415-45434 Msa1363840:intron 45.0%
ATTCACTGGTGCAGGAATCA+TGG - tig0018089:45429-45448 Msa1363840:intron 45.0%
TTCACTGGTGCAGGAATCAT+GGG - tig0018089:45430-45449 Msa1363840:intron 45.0%
TGAACACAAACCCACGCGTT+AGG - tig0018089:41576-41595 Msa1363840:three_prime_UTR 50.0%
CCATAGAATTGCCTAACGCG+TGG + tig0018089:41590-41609 None:intergenic 50.0%
CCACGCGTTAGGCAATTCTA+TGG - tig0018089:41587-41606 Msa1363840:three_prime_UTR 50.0%
CGCCAACCAAGAGAGAACTT+AGG + tig0018089:41665-41684 None:intergenic 50.0%
AAGTTCTCTCTTGGTTGGCG+TGG - tig0018089:41665-41684 Msa1363840:three_prime_UTR 50.0%
CGTCATCTTCATCTTGAGGG+TGG + tig0018089:41736-41755 None:intergenic 50.0%
CGGCGTCATCTTCATCTTGA+GGG + tig0018089:41739-41758 None:intergenic 50.0%
CCTCAAGATGAAGATGACGC+CGG - tig0018089:41737-41756 Msa1363840:CDS 50.0%
! ATTGCATGCCTTTGGACTGG+AGG + tig0018089:41775-41794 None:intergenic 50.0%
TATAGGTGAATGGTGGCACG+CGG - tig0018089:41903-41922 Msa1363840:CDS 50.0%
TACGGCAGCGTTAGTATCGT+TGG + tig0018089:41958-41977 None:intergenic 50.0%
CCGTACGCATTCAGAGGATT+AGG - tig0018089:41973-41992 Msa1363840:CDS 50.0%
ACGCATTCAGAGGATTAGGG+TGG - tig0018089:41977-41996 Msa1363840:CDS 50.0%
CGCATTCAGAGGATTAGGGT+GGG - tig0018089:41978-41997 Msa1363840:CDS 50.0%
GCATTCAGAGGATTAGGGTG+GGG - tig0018089:41979-41998 Msa1363840:CDS 50.0%
CATTCAGAGGATTAGGGTGG+GGG - tig0018089:41980-41999 Msa1363840:CDS 50.0%
GTTCTCGGAAACGGATATGG+CGG + tig0018089:42101-42120 None:intergenic 50.0%
CGAGTTCTCGGAAACGGATA+TGG + tig0018089:42104-42123 None:intergenic 50.0%
GAAATGCGGTTCTGCACGAT+TGG + tig0018089:42676-42695 None:intergenic 50.0%
!! GGTGTCACTATCATGGGAAG+TGG + tig0018089:43076-43095 None:intergenic 50.0%
GTCAATTATACCGGGACGGA+GGG + tig0018089:43154-43173 None:intergenic 50.0%
ATGGGTCAGTTATTCCGGGA+CGG - tig0018089:43350-43369 Msa1363840:intron 50.0%
TTGCTTCCGATCGTGGATGT+CGG - tig0018089:43937-43956 Msa1363840:intron 50.0%
GTGTCGTAGGGAATGGAAAG+TGG + tig0018089:44248-44267 None:intergenic 50.0%
GGCTACACCTTCCTTGTAGT+TGG - tig0018089:44298-44317 Msa1363840:intron 50.0%
CAAGGAATAGTCCCTTGGAG+GGG + tig0018089:44449-44468 None:intergenic 50.0%
CCAAGGAATAGTCCCTTGGA+GGG + tig0018089:44450-44469 None:intergenic 50.0%
TCCAAGGAATAGTCCCTTGG+AGG + tig0018089:44451-44470 None:intergenic 50.0%
GGTTCCAAGGAATAGTCCCT+TGG + tig0018089:44454-44473 None:intergenic 50.0%
! CTGCTGCAGTTTCTGGGTAT+TGG - tig0018089:44733-44752 Msa1363840:intron 50.0%
!! GCCAGATGATGGACCTTCTT+TGG - tig0018089:44800-44819 Msa1363840:intron 50.0%
GCCAAAGAAGGTCCATCATC+TGG + tig0018089:44804-44823 None:intergenic 50.0%
! GACCTTCTTTGGCTCCAACT+TGG - tig0018089:44811-44830 Msa1363840:intron 50.0%
! ACCCAAGTTGGAGCCAAAGA+AGG + tig0018089:44816-44835 None:intergenic 50.0%
CAAAGACGATAGCGAGTCCA+AGG + tig0018089:44839-44858 None:intergenic 50.0%
TCAACTCTTCGCAATCGGTC+TGG - tig0018089:44866-44885 Msa1363840:intron 50.0%
AGTGTTCGAAGAATCAGGCG+AGG + tig0018089:45226-45245 None:intergenic 50.0%
TGGGTGCATTCTCGTCCATA+AGG - tig0018089:45449-45468 Msa1363840:CDS 50.0%
GCGTGGCTAAACTCATGCCA+TGG - tig0018089:41682-41701 Msa1363840:three_prime_UTR 55.0%
TGCCATGGCAAACCCACCTA+AGG - tig0018089:41697-41716 Msa1363840:exon 55.0%
! CTCCTTAGGTGGGTTTGCCA+TGG + tig0018089:41702-41721 None:intergenic 55.0%
CCGGCGTCATCTTCATCTTG+AGG + tig0018089:41740-41759 None:intergenic 55.0%
GCTTTCGTCCTCCAGTCCAA+AGG - tig0018089:41764-41783 Msa1363840:CDS 55.0%
ATAGGTGAATGGTGGCACGC+GGG - tig0018089:41904-41923 Msa1363840:CDS 55.0%
AGAGGATTAGGGTGGGGGAT+TGG - tig0018089:41985-42004 Msa1363840:CDS 55.0%
! CATTGATGACAGGGTCGGGT+TGG - tig0018089:42737-42756 Msa1363840:CDS 55.0%
!! CGCCGGTATTGATTGCCGTT+TGG + tig0018089:42786-42805 None:intergenic 55.0%
ACGGCAATCAATACCGGCGT+TGG - tig0018089:42787-42806 Msa1363840:intron 55.0%
!! ATCAATACCGGCGTTGGCGT+AGG - tig0018089:42793-42812 Msa1363840:intron 55.0%
!! TCAATACCGGCGTTGGCGTA+GGG - tig0018089:42794-42813 Msa1363840:intron 55.0%
GGTCAATTATACCGGGACGG+AGG + tig0018089:43155-43174 None:intergenic 55.0%
GTCAGTTATTCCGGGACGGA+GGG - tig0018089:43354-43373 Msa1363840:intron 55.0%
AACAACTACTCCCTCCGTCC+CGG + tig0018089:43367-43386 None:intergenic 55.0%
CCCTCCAAGGGACTATTCCT+TGG - tig0018089:44447-44466 Msa1363840:CDS 55.0%
!! GAAGGCACTGAAGACCCTTG+AGG + tig0018089:44485-44504 None:intergenic 55.0%
ACTCTAGCACCTCCTGCAAC+TGG - tig0018089:44654-44673 Msa1363840:intron 55.0%
TTGGCTCCAACTTGGGTCCT+TGG - tig0018089:44819-44838 Msa1363840:intron 55.0%
GGAGACATCAATGGCGTGGT+TGG - tig0018089:45292-45311 Msa1363840:CDS 55.0%
!! AATGGCGTGGTTGGTGCTGT+TGG - tig0018089:45301-45320 Msa1363840:CDS 55.0%
ATGGTGGCATTCACTGGTGC+AGG - tig0018089:45421-45440 Msa1363840:intron 55.0%
CTGGAGGACGAAAGCAGCTC+CGG + tig0018089:41759-41778 None:intergenic 60.0%
TAGGTGAATGGTGGCACGCG+GGG - tig0018089:41905-41924 Msa1363840:CDS 60.0%
ACGCTGCCGTACGCATTCAG+AGG - tig0018089:41967-41986 Msa1363840:CDS 60.0%
GAAACGGATATGGCGGCGAC+CGG + tig0018089:42094-42113 None:intergenic 60.0%
AGACATCGGCGGCGAGTTCT+CGG + tig0018089:42116-42135 None:intergenic 60.0%
GAACTCGCCGCCGATGTCTT+AGG - tig0018089:42117-42136 Msa1363840:intron 60.0%
!! CAATACCGGCGTTGGCGTAG+GGG - tig0018089:42795-42814 Msa1363840:intron 60.0%
GTAGGGGCAATCCTGCTTGC+TGG - tig0018089:42811-42830 Msa1363840:intron 60.0%
!! GGAGGCATTGACCAGCAAGC+AGG + tig0018089:42825-42844 None:intergenic 60.0%
! GCTTGCTGGTCAATGCCTCC+AGG - tig0018089:42825-42844 Msa1363840:intron 60.0%
AGCAACTACTCCCTCCGTCC+CGG - tig0018089:43141-43160 Msa1363840:intron 60.0%
GGTCAGTTATTCCGGGACGG+AGG - tig0018089:43353-43372 Msa1363840:intron 60.0%
GCGAGTCCAAGGACCCAAGT+TGG + tig0018089:44828-44847 None:intergenic 60.0%
CGGCGGCGAGTTCTCGGAAA+CGG + tig0018089:42110-42129 None:intergenic 65.0%
GATTGCCCCTACGCCAACGC+CGG + tig0018089:42803-42822 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0018089 gene 41502 45729 41502 ID=Msa1363840;Name=Msa1363840
tig0018089 mRNA 41502 45729 41502 ID=Msa1363840-mRNA-1;Parent=Msa1363840;Name=Msa1363840-mRNA-1;_AED=0.12;_eAED=0.12;_QI=199|0.83|0.85|1|1|1|7|201|471
tig0018089 exon 41502 42092 41502 ID=Msa1363840-mRNA-1:exon:13722;Parent=Msa1363840-mRNA-1
tig0018089 exon 42344 42580 42344 ID=Msa1363840-mRNA-1:exon:13721;Parent=Msa1363840-mRNA-1
tig0018089 exon 42670 42800 42670 ID=Msa1363840-mRNA-1:exon:13720;Parent=Msa1363840-mRNA-1
tig0018089 exon 42894 43080 42894 ID=Msa1363840-mRNA-1:exon:13719;Parent=Msa1363840-mRNA-1
tig0018089 exon 44385 44482 44385 ID=Msa1363840-mRNA-1:exon:13718;Parent=Msa1363840-mRNA-1
tig0018089 exon 45036 45323 45036 ID=Msa1363840-mRNA-1:exon:13717;Parent=Msa1363840-mRNA-1
tig0018089 exon 45446 45729 45446 ID=Msa1363840-mRNA-1:exon:13716;Parent=Msa1363840-mRNA-1
tig0018089 five_prime_UTR 45531 45729 45531 ID=Msa1363840-mRNA-1:five_prime_utr;Parent=Msa1363840-mRNA-1
tig0018089 CDS 45446 45530 45446 ID=Msa1363840-mRNA-1:cds;Parent=Msa1363840-mRNA-1
tig0018089 CDS 45036 45323 45036 ID=Msa1363840-mRNA-1:cds;Parent=Msa1363840-mRNA-1
tig0018089 CDS 44385 44482 44385 ID=Msa1363840-mRNA-1:cds;Parent=Msa1363840-mRNA-1
tig0018089 CDS 42894 43080 42894 ID=Msa1363840-mRNA-1:cds;Parent=Msa1363840-mRNA-1
tig0018089 CDS 42670 42800 42670 ID=Msa1363840-mRNA-1:cds;Parent=Msa1363840-mRNA-1
tig0018089 CDS 42344 42580 42344 ID=Msa1363840-mRNA-1:cds;Parent=Msa1363840-mRNA-1
tig0018089 CDS 41703 42092 41703 ID=Msa1363840-mRNA-1:cds;Parent=Msa1363840-mRNA-1
tig0018089 three_prime_UTR 41502 41702 41502 ID=Msa1363840-mRNA-1:three_prime_utr;Parent=Msa1363840-mRNA-1
Gene Sequence

>Msa1363840

ATGGCAAACCCACCTAAGGAGAATCAGAATCACCACCCTCAAGATGAAGATGACGCCGGAGCTGCTTTCGTCCTCCAGTCCAAAGGTGAATGGTGGCACGCGGGGTATCATTTAACAACGGCGATTGTAGGGCCAACGATACTAACGCTGCCGTACGCATTCAGAGGATTAGGGTGGGGGATTGGAATGTTATGCCTAACAGTTATGGGTCTTGTAACCTTCTACTCTTACTGCCTCATGTCTAAAGTTCTTGATCATTGCGAAAATGCCGGTCGCCGCCATATCCGTTTCCGAGAACTCGCCGCCGATGTCTTAGGTGATGTAATCTTTACTTTGCACGTTTGCATTGTGTTCTTTTCTAATTCTTTTTCTGGGTCGGGTTGGATGTTCTATTTTGTAATCTTTATCCAAACGGCAATCAATACCGGCGTTGGCGTAGGGGCAATCCTGCTTGCTGGTCAATGCCTCCAGATCGTGTACTCCAATATTGCACCACATGGAGCATTGAAACTGTACGACTTCATAGCAATGGTGACAGTAGTCATGATAATTTTGTCTCAGCTTCCCACTTTCCATTCCCTACGACACATCAACTTGTGTTCCCTTTTTCTCAGCTTGGGCTACACCTTCCTTGTAGTTGGTGCTTGTATTCATGCAGGTACATCAAAAAATGCCCCTCCAAGGGACTATTCCTTGGAACCTAAGGAATCCTCAAGGGTCTTCAGTGCCTTCACTGCCATTTCCATAATTGCAGCAATTTTTGGGAATGGCATATTACCTGAGATACAAGCAACTCTAGCACCTCCTGCAACTGGAAAGATGATAAAAGGCCTTACTATGTGTTATGCCGTAATTTTGGTGACATTCTACTCTGCTGCAGTTTCTGGGTATTGGGTATTTGGAAACAAGTCCAACTCCAACATTTTGAAGAGTTTGATGCCAGATGATGGACCTTCTTTGGCTCCAACTTGGGTCCTTGGACTCGCTATCGTCTTTGTTCTTCTTCAACTCTTCGCAATCGGTCTGGTTTATTCTCAAGTTGCTTATGAGATAATGGAGAAGAAATCGGCAGATGTGAAGCAAGGGATGTTTTCCAAAAGAAATCTTCTTCCTCGCCTGATTCTTCGAACACTTTACATGATTTTCTGTGGATTTCTAGCAGCTATGTTGCCGTTTTTTGGAGACATCAATGGCGTGGTTGGTGCTGTTGGTTTCATTCCTCTGGATTTTGTCCTGCCTATGATTTTATACAACAAAACACACAAGCCTCCAAAATCATCATTGACTTATTGGATCAACATTTCAATCATGGTGGCATTCACTGGTGCAGGAATCATGGGTGCATTCTCGTCCATAAGGAAATTAGTTCTTGATGCTAACCAATTTAAGCTGTTTGATAGTGAAGTTGTGGATTGA
Protein sequence

>Msa1363840

MANPPKENQNHHPQDEDDAGAAFVLQSKGEWWHAGYHLTTAIVGPTILTLPYAFRGLGWGIGMLCLTVMGLVTFYSYCLMSKVLDHCENAGRRHIRFRELAADVLGDVIFTLHVCIVFFSNSFSGSGWMFYFVIFIQTAINTGVGVGAILLAGQCLQIVYSNIAPHGALKLYDFIAMVTVVMIILSQLPTFHSLRHINLCSLFLSLGYTFLVVGACIHAGTSKNAPPRDYSLEPKESSRVFSAFTAISIIAAIFGNGILPEIQATLAPPATGKMIKGLTMCYAVILVTFYSAAVSGYWVFGNKSNSNILKSLMPDDGPSLAPTWVLGLAIVFVLLQLFAIGLVYSQVAYEIMEKKSADVKQGMFSKRNLLPRLILRTLYMIFCGFLAAMLPFFGDINGVVGAVGFIPLDFVLPMILYNKTHKPPKSSLTYWINISIMVAFTGAGIMGAFSSIRKLVLDANQFKLFDSEVVD*