AlfalfaGEDB | Msa1431240

M. sativa cultivar zhongmu-4 / Msa1431240

KEGG: -
GO: -
PFAM: PF07082.14

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1431240	XP_004498831.1	70.098	408	53	7	1	340	1	407	0.0	559

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1431240	A0A1S2Y4U5	70.098	408	53	7	1	340	1	407	0.0	559

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000220	Msa1431240	0.822828	1.922370e-53	-8.615850e-47
Msa0000500	Msa1431240	0.810162	1.321701e-50	-8.615850e-47
Msa0001460	Msa1431240	0.827095	1.890370e-54	-8.615850e-47
Msa0004340	Msa1431240	0.867802	1.045979e-65	-8.615850e-47
Msa0005690	Msa1431240	0.998424	1.111928e-264	-8.615850e-47
Msa0006050	Msa1431240	0.815768	7.805135e-52	-8.615850e-47
Msa0006700	Msa1431240	0.817858	2.651280e-52	-8.615850e-47
Msa0007570	Msa1431240	0.809593	1.752403e-50	-8.615850e-47
Msa0008250	Msa1431240	0.857302	1.790223e-62	-8.615850e-47
Msa0008330	Msa1431240	0.800636	1.313021e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1431240	MtrunA17_Chr1g0150601	98.529	204	3	0	137	340	196	399	5.55e-149	425
Msa1431240	MtrunA17_Chr1g0150601	93.525	139	7	2	1	137	1	139	2.29e-85	263
Msa1431240	MtrunA17_Chr8g0369131	27.000	300	156	9	46	296	81	366	1.55e-27	112

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1431240	AT3G43540.1	53.550	338	99	4	61	340	36	373	2.58e-123	359
Msa1431240	AT3G43540.2	51.485	303	113	6	47	340	24	301	2.59e-103	306
Msa1431240	AT5G47860.1	26.695	236	119	5	81	270	100	327	2.76e-23	100

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Find 69 sgRNAs with CRISPR-Local

Find 253 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACTTACCTACTTGCCATCT+TGG	0.343767	tig0026810:-57962	None:intergenic
AAGATGGTTCTTGATTCTGT+TGG	0.514302	tig0026810:+56820	Msa1431240:CDS
AAGCCTCGTGTGGAGTCCTT+TGG	0.443070	tig0026810:+57787	Msa1431240:CDS
AAGTACCTGGTATTCTTCAA+AGG	0.530570	tig0026810:-53983	None:intergenic
AATGGTTCTGGCAAGGATTC+TGG	0.311836	tig0026810:-58041	None:intergenic
AATTCTGCAGGAAAGCGAGA+AGG	0.631851	tig0026810:+56846	Msa1431240:CDS
ACAAATATATTCATCAATCT+TGG	0.381956	tig0026810:+58287	Msa1431240:three_prime_UTR
ACAACACTTCACAATGGAAA+TGG	0.475322	tig0026810:+53934	Msa1431240:exon
ACGTTTGAACCAGTCACTAA+TGG	0.412105	tig0026810:-58059	None:intergenic
ACTGAAATCAGGAACCAAGA+TGG	0.615679	tig0026810:+57948	Msa1431240:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGTTGAATGAATTTGAGTGT+TGG	-	tig0026810:54033-54052	None:intergenic	30.0%
ATAGCTTCTGATATGTATGT+TGG	+	tig0026810:54106-54125	Msa1431240:CDS	30.0%
TTTGATTATAGCTCTTGGTT+TGG	-	tig0026810:54200-54219	None:intergenic	30.0%
GCTATAATCAAATTCATTGG+TGG	+	tig0026810:54208-54227	Msa1431240:CDS	30.0%
CTATTCCTCAACTTACTTAT+GGG	+	tig0026810:54245-54264	Msa1431240:CDS	30.0%
TGACAAGTTTCATTCTTGTT+TGG	+	tig0026810:54482-54501	Msa1431240:intron	30.0%
TAGTTTAGTACCAATCTTAC+TGG	+	tig0026810:54776-54795	Msa1431240:intron	30.0%
TACCAATCTTACTGGAATTA+AGG	+	tig0026810:54784-54803	Msa1431240:intron	30.0%
TACCTTAATTCCAGTAAGAT+TGG	-	tig0026810:54789-54808	None:intergenic	30.0%
CAGAAAAATAACCAAACCTT+AGG	-	tig0026810:54898-54917	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0026810	gene	53855	58324	53855	ID=Msa1431240;Name=Msa1431240
tig0026810	mRNA	53855	58324	53855	ID=Msa1431240-mRNA-1;Parent=Msa1431240;Name=Msa1431240-mRNA-1;_AED=0.15;_eAED=0.15;_QI=92\|0.87\|0.88\|1\|0.87\|0.77\|9\|195\|340
tig0026810	exon	53855	54266	53855	ID=Msa1431240-mRNA-1:exon:7446;Parent=Msa1431240-mRNA-1
tig0026810	exon	54386	54476	54386	ID=Msa1431240-mRNA-1:exon:7447;Parent=Msa1431240-mRNA-1
tig0026810	exon	55153	55215	55153	ID=Msa1431240-mRNA-1:exon:7448;Parent=Msa1431240-mRNA-1
tig0026810	exon	56003	56078	56003	ID=Msa1431240-mRNA-1:exon:7449;Parent=Msa1431240-mRNA-1
tig0026810	exon	56809	56924	56809	ID=Msa1431240-mRNA-1:exon:7450;Parent=Msa1431240-mRNA-1
tig0026810	exon	57548	57637	57548	ID=Msa1431240-mRNA-1:exon:7451;Parent=Msa1431240-mRNA-1
tig0026810	exon	57733	57864	57733	ID=Msa1431240-mRNA-1:exon:7452;Parent=Msa1431240-mRNA-1
tig0026810	exon	57959	58211	57959	ID=Msa1431240-mRNA-1:exon:7453;Parent=Msa1431240-mRNA-1
tig0026810	exon	58248	58324	58248	ID=Msa1431240-mRNA-1:exon:7454;Parent=Msa1431240-mRNA-1
tig0026810	five_prime_UTR	53855	53946	53855	ID=Msa1431240-mRNA-1:five_prime_utr;Parent=Msa1431240-mRNA-1
tig0026810	CDS	53947	54266	53947	ID=Msa1431240-mRNA-1:cds;Parent=Msa1431240-mRNA-1
tig0026810	CDS	54386	54476	54386	ID=Msa1431240-mRNA-1:cds;Parent=Msa1431240-mRNA-1
tig0026810	CDS	55153	55215	55153	ID=Msa1431240-mRNA-1:cds;Parent=Msa1431240-mRNA-1
tig0026810	CDS	56003	56078	56003	ID=Msa1431240-mRNA-1:cds;Parent=Msa1431240-mRNA-1
tig0026810	CDS	56809	56924	56809	ID=Msa1431240-mRNA-1:cds;Parent=Msa1431240-mRNA-1
tig0026810	CDS	57548	57637	57548	ID=Msa1431240-mRNA-1:cds;Parent=Msa1431240-mRNA-1
tig0026810	CDS	57733	57864	57733	ID=Msa1431240-mRNA-1:cds;Parent=Msa1431240-mRNA-1
tig0026810	CDS	57959	58093	57959	ID=Msa1431240-mRNA-1:cds;Parent=Msa1431240-mRNA-1
tig0026810	three_prime_UTR	58094	58211	58094	ID=Msa1431240-mRNA-1:three_prime_utr;Parent=Msa1431240-mRNA-1
tig0026810	three_prime_UTR	58248	58324	58248	ID=Msa1431240-mRNA-1:three_prime_utr;Parent=Msa1431240-mRNA-1

Gene Sequence

>Msa1431240

ATGGAAATGGCAACCTCAAATTTCAAATTCACTCTCCCTTTGAAGAATACCAGGTACTTGTCTATGTTTCATGGATTGAAACACCAACACTCAAATTCATTCAACAAATGTTTCAAACTTCAATCTTTGTCTACTAGAATAGTGTCAGCTACTACAAACATAGCTTCTGATATGTATGTTGGAAGAAACAATAGTAACAAGATTTACACAAACTTGAATTCTTGTCTTGTGATTCCACCACCTACTTCCACCAAACCAAGAGCTATAATCAAATTCATTGGTGGTGCTTTCGTTGGTGCTATTCCTCAACTTACTTATGGATATCTTATAGAATTATTGGCTAAGGAAGGTTTTGTTGTTGTGGTGGTGCCTTATAATGTGACATTTGATCATTCTCTAGCTGCTAAACAAGCTAATGCTGTAATCGCTTACAACAACAGACCAGCAACAGAGGCGGTTCCTTACTTTGAGCAGCTAGGTCCTGCTGTCAGCCAGATGATGCCAATAGTGGAAGCAAATCCAATTTACTCGATAGCTAGAAATGCACCAGGAGATGCATGGAAGATGGTTCTTGATTCTGTTGGATCAATTCTGCAGGAAAGCGAGAAGGAAGTTCTGAATTCATTAACTAAATTTGTTGATCAGCTGCCTTTGGTAATGAATGAGGTTACACAAGGAGTCTCAGAGTTCAAGCCAACACCATCTGAAAATCGTGATTGCTTTAAAAGTTCGTACAATGTCAAACACACATTATTGGTGCAATTCAATTCCGACACAATTGATGAAACAAATATTCTTGAAGAAACACTCAAGCCTCGTGTGGAGTCCTTTGGTGGGACAATAGAAAAAGTAGGATTGAGTGGTAACCACATCACACCATGCATCCAGGAACCAAGATGGCAAGTAGGTAAGTTGTATACTCCAGCAGATGCTGTAGCTCAAAGGCTTAAATCTTTTTCTTTGAGTGATACCAGAATCCTTGCCAGAACCATTAGTGACTGGTTCAAACGTTTTGAGGATTGA

Protein sequence

>Msa1431240

MEMATSNFKFTLPLKNTRYLSMFHGLKHQHSNSFNKCFKLQSLSTRIVSATTNIASDMYVGRNNSNKIYTNLNSCLVIPPPTSTKPRAIIKFIGGAFVGAIPQLTYGYLIELLAKEGFVVVVVPYNVTFDHSLAAKQANAVIAYNNRPATEAVPYFEQLGPAVSQMMPIVEANPIYSIARNAPGDAWKMVLDSVGSILQESEKEVLNSLTKFVDQLPLVMNEVTQGVSEFKPTPSENRDCFKSSYNVKHTLLVQFNSDTIDETNILEETLKPRVESFGGTIEKVGLSGNHITPCIQEPRWQVGKLYTPADAVAQRLKSFSLSDTRILARTISDWFKRFED*