AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1372210

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1372210 MtrunA17_Chr2g0287651 42.816 696 379 6 93 783 63 744 0.0 584
Msa1372210 MtrunA17_Chr1g0203051 34.396 596 368 6 68 648 34 621 3.72e-116 371
Msa1372210 MtrunA17_Chr1g0202341 32.538 587 373 10 104 676 50 627 3.89e-96 318
Msa1372210 MtrunA17_Chr5g0448761 32.867 572 359 10 109 663 64 627 1.13e-93 315
Msa1372210 MtrunA17_Chr2g0324011 29.181 586 397 6 109 682 14 593 3.36e-86 289
Msa1372210 MtrunA17_Chr2g0296781 29.204 678 433 15 109 764 1 653 1.73e-84 288
Msa1372210 MtrunA17_Chr8g0363841 28.037 642 423 12 101 716 9 637 1.76e-83 284
Msa1372210 MtrunA17_Chr5g0441581 28.346 635 408 12 109 724 36 642 7.79e-83 283
Msa1372210 MtrunA17_Chr1g0198761 31.023 577 367 11 111 665 53 620 6.15e-80 276
Msa1372210 MtrunA17_Chr3g0082121 29.934 608 397 12 108 704 46 635 7.76e-79 271
Msa1372210 MtrunA17_Chr6g0477261 34.813 428 264 6 245 661 22 445 1.78e-78 270
Msa1372210 MtrunA17_Chr1g0203721 28.205 624 427 12 111 724 69 681 5.83e-76 268
Msa1372210 MtrunA17_Chr1g0207551 30.660 561 372 9 109 661 38 589 2.34e-74 261
Msa1372210 MtrunA17_Chr6g0458391 27.866 567 365 11 109 661 229 765 8.47e-72 253
Msa1372210 MtrunA17_Chr3g0080351 27.724 624 384 13 100 699 71 651 4.99e-71 253
Msa1372210 MtrunA17_Chr1g0196541 30.420 572 363 15 109 661 1 556 1.13e-70 249
Msa1372210 MtrunA17_Chr2g0329031 25.932 617 410 11 92 694 1 584 1.44e-68 244
Msa1372210 MtrunA17_Chr5g0440451 27.200 625 405 17 109 709 47 645 8.54e-65 236
Msa1372210 MtrunA17_Chr3g0118611 29.412 510 326 12 211 709 56 542 1.15e-63 230
Msa1372210 MtrunA17_Chr5g0448511 30.402 398 267 5 271 662 5 398 1.52e-63 225
Msa1372210 MtrunA17_Chr2g0276841 27.360 625 424 14 111 721 76 684 4.27e-63 227
Msa1372210 MtrunA17_Chr5g0431001 31.915 423 262 8 111 512 67 484 1.87e-62 221
Msa1372210 MtrunA17_Chr2g0290391 26.929 661 411 17 109 730 55 682 8.18e-61 221
Msa1372210 MtrunA17_Chr2g0291581 27.592 569 374 13 109 661 75 621 2.85e-60 219
Msa1372210 MtrunA17_Chr2g0287061 27.558 606 388 14 110 709 188 748 1.12e-58 216
Msa1372210 MtrunA17_Chr2g0291591 30.247 486 313 10 106 580 65 535 1.07e-57 209
Msa1372210 MtrunA17_Chr2g0294841 27.719 570 383 13 111 662 51 609 2.23e-57 212
Msa1372210 MtrunA17_Chr1g0164591 32.131 305 193 3 192 491 12 307 1.58e-49 178
Msa1372210 MtrunA17_Chr2g0308841 33.051 236 156 1 436 671 4 237 1.32e-38 145
Msa1372210 MtrunA17_Chr7g0258861 31.579 285 176 5 109 382 14 290 1.03e-37 144
Msa1372210 MtrunA17_Chr1g0149131 33.636 110 68 1 383 487 1 110 2.23e-15 73.6
Msa1372210 MtrunA17_Chr8g0340801 33.945 109 70 1 213 321 1 107 3.96e-14 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1372210 AT4G38180.1 31.269 646 421 12 33 665 3 638 1.77e-92 312
Msa1372210 AT4G15090.1 29.552 670 442 14 57 711 2 656 1.02e-84 291
Msa1372210 AT4G15090.5 29.552 670 442 14 57 711 2 656 1.02e-84 291
Msa1372210 AT4G15090.3 29.552 670 442 14 57 711 2 656 1.02e-84 291
Msa1372210 AT4G15090.2 29.552 670 442 14 57 711 2 656 1.02e-84 291
Msa1372210 AT4G15090.4 29.552 670 442 14 57 711 2 656 1.02e-84 291
Msa1372210 AT3G22170.2 26.599 688 441 14 56 720 30 676 1.30e-71 255
Msa1372210 AT3G22170.1 26.599 688 441 14 56 720 30 676 1.30e-71 255
Msa1372210 AT2G27110.2 26.260 655 414 16 63 703 14 613 4.55e-67 242
Msa1372210 AT2G27110.1 26.260 655 414 16 63 703 14 613 4.55e-67 242
Msa1372210 AT5G28530.1 28.423 577 381 11 111 667 61 625 3.33e-64 231
Msa1372210 AT3G06250.2 26.942 605 391 11 109 709 194 751 1.88e-63 230
Msa1372210 AT3G06250.1 26.942 605 391 11 109 709 194 751 1.88e-63 230
Msa1372210 AT3G06250.3 26.942 605 391 11 109 709 194 751 1.88e-63 230
Msa1372210 AT1G10240.2 27.244 635 412 13 111 721 54 662 2.82e-63 228
Msa1372210 AT1G10240.1 27.244 635 412 13 111 721 54 662 2.82e-63 228
Msa1372210 AT5G28530.3 28.076 577 379 11 111 667 61 621 4.92e-63 227
Msa1372210 AT5G28530.2 28.076 577 379 11 111 667 61 621 4.92e-63 227
Msa1372210 AT1G52520.1 26.435 662 437 16 30 666 7 643 2.00e-62 226
Msa1372210 AT2G27110.3 30.485 433 279 9 278 703 51 468 3.34e-62 225
Msa1372210 AT4G19990.7 27.451 612 375 14 83 665 6 577 5.08e-62 226
Msa1372210 AT4G19990.6 27.556 577 358 12 110 665 26 563 5.69e-62 226
Msa1372210 AT4G19990.2 27.556 577 358 12 110 665 26 563 5.69e-62 226
Msa1372210 AT4G19990.3 27.903 577 356 13 110 665 26 563 5.73e-62 225
Msa1372210 AT4G19990.4 27.903 577 356 13 110 665 26 563 5.73e-62 225
Msa1372210 AT4G19990.5 27.903 577 356 13 110 665 26 563 5.92e-62 225
Msa1372210 AT1G76320.1 26.876 573 376 13 109 664 1 547 7.46e-62 225
Msa1372210 AT1G76320.4 26.876 573 376 13 109 664 1 547 7.46e-62 225
Msa1372210 AT1G76320.2 26.876 573 376 13 109 664 1 547 8.11e-62 224
Msa1372210 AT1G76320.3 26.876 573 376 13 109 664 1 547 1.16e-61 223
Msa1372210 AT1G80010.1 28.155 618 387 20 84 670 79 670 3.21e-61 223
Msa1372210 AT5G18960.2 26.568 606 395 13 109 709 215 775 2.77e-59 218
Msa1372210 AT5G18960.1 26.568 606 395 13 109 709 215 775 2.77e-59 218
Msa1372210 AT4G19990.1 29.825 399 272 4 273 665 110 506 2.99e-59 216
Msa1372210 AT2G32250.8 26.580 617 367 14 109 701 1 555 1.16e-55 207
Msa1372210 AT2G32250.2 26.742 617 366 14 109 701 42 596 1.32e-55 207
Msa1372210 AT2G32250.4 26.742 617 366 14 109 701 42 596 1.32e-55 207
Msa1372210 AT2G32250.6 26.742 617 366 14 109 701 42 596 1.41e-55 207
Msa1372210 AT2G32250.1 26.742 617 366 14 109 701 42 596 1.41e-55 207
Msa1372210 AT2G32250.3 26.742 617 366 14 109 701 42 596 1.41e-55 207
Msa1372210 AT2G32250.5 26.742 617 366 14 109 701 42 596 1.43e-55 207
Msa1372210 AT2G32250.7 26.580 617 367 14 109 701 1 555 1.63e-55 207
Msa1372210 AT4G38170.1 28.103 427 278 9 310 720 45 458 4.32e-54 198
Msa1372210 AT5G28530.4 25.397 567 348 14 111 667 61 562 1.93e-43 168
Msa1372210 AT5G28530.5 25.044 567 346 14 111 667 61 558 2.27e-42 165

Find 175 sgRNAs with CRISPR-Local

Find 221 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAACTGATCCACCATTATT+TGG 0.155571 tig0019271:+139683 None:intergenic
CAAAGGCGATGCCTTGTATT+TGG 0.159430 tig0019271:+141429 None:intergenic
TCAATGTTGATTGTGATATT+TGG 0.171428 tig0019271:+139920 None:intergenic
CTCTTGCAAATCAGACTTTC+AGG 0.192125 tig0019271:+140709 None:intergenic
TCACTCAATTTACGGTAATT+AGG 0.235603 tig0019271:+142008 None:intergenic
CCCATAGTTTCTCAATGTTT+TGG 0.242135 tig0019271:+139408 None:intergenic
ATATCTTCAGTATCACTATC+AGG 0.265411 tig0019271:+142500 None:intergenic
TATCAACTTCACTCAATTTA+CGG 0.276736 tig0019271:+142000 None:intergenic
ATCACTCGCAACAAATTTAC+TGG 0.280329 tig0019271:+140456 None:intergenic
CCATCAGTAATCACCGCTTT+TGG 0.302214 tig0019271:+141489 None:intergenic
TCCATACTTGTTCTTTCTAT+AGG 0.305560 tig0019271:+141650 None:intergenic
TAACACCATACCCAACATTA+TGG 0.313783 tig0019271:+139732 None:intergenic
AGGACTTGGGTTTATCAAAA+AGG 0.326599 tig0019271:-141892 Msa1372210:CDS
CAGTGTTAGAATGTGATGAT+AGG 0.338987 tig0019271:+139636 None:intergenic
TGATAAACCCAAGTCCTTCA+TGG 0.346028 tig0019271:+141898 None:intergenic
AGATGATGATATAAGGGATA+TGG 0.347376 tig0019271:-142345 Msa1372210:CDS
ACCTATAGAAAGAACAAGTA+TGG 0.349144 tig0019271:-141651 Msa1372210:CDS
ACGACTTTGTTCGTACGATT+TGG 0.356788 tig0019271:-142097 Msa1372210:CDS
ACACCAATTCCAGCAAGTTC+TGG 0.360337 tig0019271:+140301 None:intergenic
ATGTGACAGATGATGATATA+AGG 0.367162 tig0019271:-142352 Msa1372210:CDS
GAGTTTACATTTACAGATGA+TGG 0.367609 tig0019271:-141756 Msa1372210:CDS
CTCGGAACATTGTGTGTTCC+AGG 0.370631 tig0019271:+139587 None:intergenic
TAAGAGCGCAAGTGATTATA+AGG 0.371814 tig0019271:-140557 Msa1372210:CDS
AGGTTTGACTATGAGTGTTT+TGG 0.377109 tig0019271:-141696 Msa1372210:CDS
TTACCCACCTGTTGTTTGAT+AGG 0.378005 tig0019271:+141268 None:intergenic
TGTAAGAACATGTCATATCT+TGG 0.387474 tig0019271:-141946 Msa1372210:CDS
TGTTCTAATTGTAGAAATCC+TGG 0.392101 tig0019271:-140343 Msa1372210:CDS
CACTATGGGGTGCCAAATAA+TGG 0.393131 tig0019271:-139695 Msa1372210:CDS
ATCCATGTTGTCATACTTCA+TGG 0.394339 tig0019271:+140738 None:intergenic
ACTGGAACACACAATGTTCC+TGG 0.398163 tig0019271:-139605 Msa1372210:CDS
ATCATTCGCAACAAATTTGT+TGG 0.403036 tig0019271:+140429 None:intergenic
TCTAACACTGCTCTGCATAC+TGG 0.407068 tig0019271:-139623 Msa1372210:CDS
ATATAATTTCACGGATCATC+AGG 0.409639 tig0019271:-141865 Msa1372210:CDS
TTTGGGCAGATTCTACTAGT+AGG 0.409763 tig0019271:-141716 Msa1372210:CDS
GATTATAAGGAGGCTCTAGA+AGG 0.413898 tig0019271:-140544 Msa1372210:CDS
GATTCATTATCAGACTCAAT+AGG 0.414121 tig0019271:+140247 None:intergenic
GCACTGGTATTACACGTAAG+AGG 0.418801 tig0019271:-140039 Msa1372210:CDS
CAAGGCATCGCCTTTGCGCC+TGG 0.420887 tig0019271:-141422 Msa1372210:CDS
AACAAATACAAGCACTAGAA+AGG 0.422563 tig0019271:-139960 Msa1372210:CDS
TGTCCAGAACTTGCTGGAAT+TGG 0.422787 tig0019271:-140304 Msa1372210:CDS
AAAGTTTAAAGATGTCAAAA+AGG 0.436388 tig0019271:-140959 Msa1372210:CDS
TACGGTAATTAGGCATCAAA+TGG 0.442752 tig0019271:+142018 None:intergenic
CGCAGAACAATTAGTTCGAA+TGG 0.445789 tig0019271:+142136 None:intergenic
AAGGGTGGCCATGAAGGACT+TGG 0.449428 tig0019271:-141906 Msa1372210:CDS
TAAATTTGTTGCGAGTGATA+AGG 0.460501 tig0019271:-140452 Msa1372210:CDS
AAATGGACATGACGAGGATA+AGG 0.462491 tig0019271:-140479 Msa1372210:CDS
ATACTTGTTCTTTCTATAGG+TGG 0.464692 tig0019271:+141653 None:intergenic
GTAAGAACATGTCATATCTT+GGG 0.464989 tig0019271:-141945 Msa1372210:CDS
GCGTCAATTAGTATGCAATA+AGG 0.467578 tig0019271:-142210 Msa1372210:CDS
AACGCAAAGGAGCTAATTCA+TGG 0.469712 tig0019271:+142045 None:intergenic
AGTGTTAGAATGTGATGATA+GGG 0.470693 tig0019271:+139637 None:intergenic
TTCGAATGGTTAGCGTAGAT+CGG 0.471186 tig0019271:+142150 None:intergenic
AATAGCCGTCATGAGGGAAA+TGG 0.477370 tig0019271:-140496 Msa1372210:CDS
CAAGGGAAAGAGAACTTTGA+TGG 0.478759 tig0019271:-142392 Msa1372210:CDS
CGGACGTGTCCAGAACTTGC+TGG 0.483560 tig0019271:-140310 Msa1372210:CDS
AAAGGACTTATATAATTTCA+CGG 0.493961 tig0019271:-141874 Msa1372210:CDS
GAAATCCTGGGCACACAATA+CGG 0.497533 tig0019271:-140330 Msa1372210:CDS
CACTCTGTAGCAGTGTCAAA+TGG 0.499398 tig0019271:-140082 Msa1372210:CDS
CCAAAAGCGGTGATTACTGA+TGG 0.502215 tig0019271:-141489 Msa1372210:CDS
ATGTGAAGCGTGATTCTAAT+GGG 0.504223 tig0019271:-142244 Msa1372210:CDS
AATGGTGTGACCCGTGACTT+TGG 0.505092 tig0019271:-139803 Msa1372210:CDS
GCAAGGGTTAAAATACAAGA+TGG 0.508614 tig0019271:-141843 Msa1372210:CDS
GTATCACTATGTACATCAAA+AGG 0.509968 tig0019271:+142623 None:intergenic
GGTCAAAAGGGTGGCCATGA+AGG 0.510809 tig0019271:-141912 Msa1372210:CDS
TTTGAGTTACTTCCAAGGTA+AGG 0.511685 tig0019271:-141805 Msa1372210:CDS
CCACGGGTTTGAAGTAAGAA+AGG 0.512169 tig0019271:-142270 Msa1372210:CDS
TTAGAACAACATCTTGTCCT+TGG 0.513299 tig0019271:+140358 None:intergenic
TGTGATGATAGGGTAGTTGT+CGG 0.517812 tig0019271:+139647 None:intergenic
AGTTTGAATCTGAGTGGAAG+AGG 0.520947 tig0019271:-141308 Msa1372210:CDS
ATTTAGTCTGCAACTTGTCA+AGG 0.524883 tig0019271:+140821 None:intergenic
CAAGGGTTAAAATACAAGAT+GGG 0.527779 tig0019271:-141842 Msa1372210:CDS
AACAACGATGTTCGTACTGA+TGG 0.529175 tig0019271:-142464 Msa1372210:CDS
AAAGTCTGATTTGCAAGAGA+TGG 0.531904 tig0019271:-140705 Msa1372210:CDS
GATGTGAAGCGTGATTCTAA+TGG 0.532287 tig0019271:-142245 Msa1372210:CDS
GTTCTAATTGTAGAAATCCT+GGG 0.533951 tig0019271:-140342 Msa1372210:CDS
AAGTGCCCCTCGACGAAACA+TGG 0.534361 tig0019271:+140615 None:intergenic
CACGTCCGTATTGTGTGCCC+AGG 0.534840 tig0019271:+140325 None:intergenic
ACAAATACAAGCACTAGAAA+GGG 0.535750 tig0019271:-139959 Msa1372210:CDS
AGCATTTCGATGCAAATGCC+AGG 0.536176 tig0019271:+141404 None:intergenic
GTAGTTGTCGGAGGGAATTG+TGG 0.539742 tig0019271:+139659 None:intergenic
TTTATCGGTTGAAGAGAATG+TGG 0.541414 tig0019271:-141239 Msa1372210:CDS
GAGTTGTGTTTCCAAATACA+AGG 0.541485 tig0019271:-141440 Msa1372210:CDS
AATAAGGAGGGTGTTAATGA+TGG 0.543414 tig0019271:-142194 Msa1372210:CDS
AAGACAGCCATTGTGCAAGT+TGG 0.545484 tig0019271:-139757 Msa1372210:CDS
AGCTAATTCATGGTTGTGAG+AGG 0.545517 tig0019271:+142055 None:intergenic
TATCAGTATCAGCCTTACCT+TGG 0.546983 tig0019271:+141793 None:intergenic
ACTTCATGGCACAAATAATG+TGG 0.547507 tig0019271:+140752 None:intergenic
CAAAAGCGGTGATTACTGAT+GGG 0.550752 tig0019271:-141488 Msa1372210:CDS
TTGACCTATCAAACAACAGG+TGG 0.553778 tig0019271:-141272 Msa1372210:CDS
GATGCAAATGCCAGGCGCAA+AGG 0.554082 tig0019271:+141412 None:intergenic
TCGTCATTTGCCTCCCAAGA+AGG 0.555407 tig0019271:-140655 Msa1372210:CDS
GTTTGAATCTGAGTGGAAGA+GGG 0.557830 tig0019271:-141307 Msa1372210:CDS
CACTGGTATTACACGTAAGA+GGG 0.559146 tig0019271:-140038 Msa1372210:CDS
GGTGTCGTGTCTGAAGATCA+AGG 0.560464 tig0019271:-140283 Msa1372210:CDS
CGAGATGTTGAGAAAAGCAC+TGG 0.563759 tig0019271:-140055 Msa1372210:CDS
TTCATCATTACCTTCTTGGG+AGG 0.565482 tig0019271:+140645 None:intergenic
GTCTGAAGATCAAGGGGAGA+AGG 0.567087 tig0019271:-140275 Msa1372210:CDS
TATCTTCAGTATCACTATCA+GGG 0.570319 tig0019271:+142501 None:intergenic
TATTGTTTGCAAAATACCAC+GGG 0.573778 tig0019271:-142286 Msa1372210:CDS
CCTTTGACATTCACCATCAG+TGG 0.574175 tig0019271:+140406 None:intergenic
GTGGAATTTAACCATGGTAA+TGG 0.576772 tig0019271:-142428 Msa1372210:CDS
GCACAAATAATGTGGGAACA+TGG 0.578248 tig0019271:+140760 None:intergenic
TGTGACAGATGATGATATAA+GGG 0.581249 tig0019271:-142351 Msa1372210:CDS
AAATGACCATGTTTCGTCGA+GGG 0.581274 tig0019271:-140621 Msa1372210:CDS
GTGTCGTGTCTGAAGATCAA+GGG 0.581492 tig0019271:-140282 Msa1372210:CDS
GCTAATTCATGGTTGTGAGA+GGG 0.581523 tig0019271:+142056 None:intergenic
TCAATTAGTATGCAATAAGG+AGG 0.584633 tig0019271:-142207 Msa1372210:CDS
AATGACCATGTTTCGTCGAG+GGG 0.585541 tig0019271:-140620 Msa1372210:CDS
GCAAGTTGGAACCATAATGT+TGG 0.585606 tig0019271:-139743 Msa1372210:CDS
TAAGATAAATAGCCGTCATG+AGG 0.585792 tig0019271:-140503 Msa1372210:CDS
AGGGTGGCCATGAAGGACTT+GGG 0.586883 tig0019271:-141905 Msa1372210:CDS
GAAGCTTCAAAGATATTCAC+CGG 0.587720 tig0019271:-140985 Msa1372210:CDS
AAGATAAATAGCCGTCATGA+GGG 0.587755 tig0019271:-140502 Msa1372210:CDS
ATACTCACCGGTGTATAACT+CGG 0.588936 tig0019271:+139569 None:intergenic
ATGATAGGGTAGTTGTCGGA+GGG 0.591320 tig0019271:+139651 None:intergenic
ATTTCACGGATCATCAGGCA+AGG 0.592596 tig0019271:-141860 Msa1372210:CDS
TGGAACCATAATGTTGGGTA+TGG 0.594448 tig0019271:-139737 Msa1372210:CDS
CTTCATGGCACAAATAATGT+GGG 0.594948 tig0019271:+140753 None:intergenic
TTATCGGTTGAAGAGAATGT+GGG 0.595180 tig0019271:-141238 Msa1372210:CDS
TATGAGTGCGTTGAAGAATG+TGG 0.597255 tig0019271:-139466 Msa1372210:CDS
GAACGAGATGAAGATGAGGA+TGG 0.598765 tig0019271:-140904 Msa1372210:CDS
GTATTGTTTGCAAAATACCA+CGG 0.599210 tig0019271:-142287 Msa1372210:CDS
CAAGTTGGAACCATAATGTT+GGG 0.599464 tig0019271:-139742 Msa1372210:CDS
GTCAATGCAGTTGTCAGCAT+AGG 0.607551 tig0019271:+141021 None:intergenic
CTGAAAAGTTTGAATCTGAG+TGG 0.609008 tig0019271:-141314 Msa1372210:CDS
AAGGGTTAAAATACAAGATG+GGG 0.610552 tig0019271:-141841 Msa1372210:CDS
AAACATCGCAGTGACTATTG+TGG 0.611836 tig0019271:-139432 Msa1372210:CDS
ATTATAAGGAGGCTCTAGAA+GGG 0.613050 tig0019271:-140543 Msa1372210:CDS
GACACTGATAGTGATACAGA+TGG 0.614810 tig0019271:-142566 Msa1372210:CDS
GCTACTTTGAGTTACTTCCA+AGG 0.617165 tig0019271:-141810 Msa1372210:CDS
GTTGATAAACAAGTTGCTAG+TGG 0.617676 tig0019271:-141984 Msa1372210:CDS
TGATAGTGATACAGATGGGG+TGG 0.618742 tig0019271:-142561 Msa1372210:CDS
CATGTCCATTTCCCTCATGA+CGG 0.619225 tig0019271:+140491 None:intergenic
GATGATAGGGTAGTTGTCGG+AGG 0.619255 tig0019271:+139650 None:intergenic
AAATGGTTGTTTCTCCATGG+TGG 0.622494 tig0019271:+141598 None:intergenic
CATTGAACGAGATGAAGATG+AGG 0.623154 tig0019271:-140908 Msa1372210:CDS
ACATAGTGATACAGATGAGG+TGG 0.623452 tig0019271:-142612 Msa1372210:CDS
CCACTGATGGTGAATGTCAA+AGG 0.624002 tig0019271:-140406 Msa1372210:CDS
TATGGTTCCAACTTGCACAA+TGG 0.627946 tig0019271:+139750 None:intergenic
TTTCACGGATCATCAGGCAA+GGG 0.637022 tig0019271:-141859 Msa1372210:CDS
AAAAGCGGTGATTACTGATG+GGG 0.637407 tig0019271:-141487 Msa1372210:CDS
TTTAGTGGATATAACCACCA+TGG 0.638528 tig0019271:-141612 Msa1372210:CDS
AGAACGACATCAATCTGTGA+AGG 0.639278 tig0019271:-141177 Msa1372210:CDS
CCTTTCTTACTTCAAACCCG+TGG 0.639832 tig0019271:+142270 None:intergenic
CATTGCAAACACCATTACCA+TGG 0.642035 tig0019271:+142417 None:intergenic
TATGGGGTGCCAAATAATGG+TGG 0.646881 tig0019271:-139692 Msa1372210:CDS
AATGGTGTTTGCAATGAGCA+AGG 0.648235 tig0019271:-142410 Msa1372210:CDS
GCTAGTGGTCTACATTCGCA+AGG 0.649023 tig0019271:-141969 Msa1372210:CDS
AACAATAGAGACATACAAGT+GGG 0.649289 tig0019271:-141550 Msa1372210:CDS
ATCAAATGGACAAAACGCAA+AGG 0.651947 tig0019271:+142032 None:intergenic
AAAATGACCATGTTTCGTCG+AGG 0.652304 tig0019271:-140622 Msa1372210:CDS
TGACCTATCAAACAACAGGT+GGG 0.653955 tig0019271:-141271 Msa1372210:CDS
ATGGTGTTTGCAATGAGCAA+GGG 0.657603 tig0019271:-142409 Msa1372210:CDS
TCTGTAGCAGTGTCAAATGG+TGG 0.657609 tig0019271:-140079 Msa1372210:CDS
TGATGGAGATGCAGACGGTG+TGG 0.660310 tig0019271:-142447 Msa1372210:CDS
ACACTGATAGTGATACAGAT+GGG 0.663559 tig0019271:-142565 Msa1372210:CDS
TGTCGTGTCTGAAGATCAAG+GGG 0.668190 tig0019271:-140281 Msa1372210:CDS
AAACAATAGAGACATACAAG+TGG 0.671084 tig0019271:-141551 Msa1372210:CDS
TGCCATGAAGTATGACAACA+TGG 0.671427 tig0019271:-140740 Msa1372210:CDS
GTATGACAAACATCCAAAAG+CGG 0.671446 tig0019271:-141502 Msa1372210:CDS
TGAATGTCAAAGGTGCCCGT+AGG 0.674674 tig0019271:-140396 Msa1372210:CDS
CAATTAGTATGCAATAAGGA+GGG 0.675920 tig0019271:-142206 Msa1372210:CDS
TGTAGACGCTTTGAACGTAG+AGG 0.677604 tig0019271:-140787 Msa1372210:CDS
CAATGTTCCGAGTTATACAC+CGG 0.682160 tig0019271:-139576 Msa1372210:intron
CATTTGACACTGCTACAGAG+TGG 0.682675 tig0019271:+140083 None:intergenic
GAGCGCAAGTGATTATAAGG+AGG 0.683366 tig0019271:-140554 Msa1372210:CDS
TGTTGCGAATGATCCACTGA+TGG 0.687875 tig0019271:-140419 Msa1372210:CDS
GACGGTGTGGAATTTAACCA+TGG 0.688761 tig0019271:-142434 Msa1372210:CDS
TGAGGGAAATGGACATGACG+AGG 0.691164 tig0019271:-140485 Msa1372210:CDS
CGTACTGATGGAGATGCAGA+CGG 0.711480 tig0019271:-142452 Msa1372210:CDS
ACATAGACTATTGAACGCAG+CGG 0.718507 tig0019271:+140591 None:intergenic
CACTGATAGTGATACAGATG+GGG 0.724418 tig0019271:-142564 Msa1372210:CDS
TGTACATAGTGATACAGATG+AGG 0.739686 tig0019271:-142615 Msa1372210:CDS
AATTATGCAAGAAATCCACA+AGG 0.743577 tig0019271:+141112 None:intergenic
GGATATGGAATTCAACACAG+AGG 0.763384 tig0019271:-142330 Msa1372210:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGAAATTGTATATGATATTT+TGG - tig0019271:141915-141934 Msa1372210:CDS 15.0%
!!! AATTATTTTAATATGCACTA+TGG - tig0019271:142359-142378 Msa1372210:CDS 15.0%
!!! ATTATTTTAATATGCACTAT+GGG - tig0019271:142360-142379 Msa1372210:CDS 15.0%
!!! TCATAAACTGTTTTAAATAT+AGG + tig0019271:142588-142607 None:intergenic 15.0%
!! AAAGGACTTATATAATTTCA+CGG - tig0019271:140195-140214 Msa1372210:intron 20.0%
!! AAAGTTTAAAGATGTCAAAA+AGG - tig0019271:141110-141129 Msa1372210:CDS 20.0%
!!! ATTTTCAAGGTTAATGTATT+TGG - tig0019271:141198-141217 Msa1372210:CDS 20.0%
!!! TTATTTTAATATGCACTATG+GGG - tig0019271:142361-142380 Msa1372210:CDS 20.0%
! GATGGAAAAAATAAGAAGAA+TGG - tig0019271:139893-139912 Msa1372210:intron 25.0%
! TATCAACTTCACTCAATTTA+CGG + tig0019271:140072-140091 None:intergenic 25.0%
! ATATCCACTAAAAATGACAA+GGG + tig0019271:140449-140468 None:intergenic 25.0%
! TATATCCACTAAAAATGACA+AGG + tig0019271:140450-140469 None:intergenic 25.0%
! AATTAAAGCACAAGCAAAAA+TGG + tig0019271:140491-140510 None:intergenic 25.0%
!!! GGTTCTAAATTTGTTTTCAA+AGG - tig0019271:140540-140559 Msa1372210:CDS 25.0%
! GGTGGGTAAAAAAAATTTAT+CGG - tig0019271:140815-140834 Msa1372210:CDS 25.0%
!! GACATCTTTAAACTTTTTAC+CGG + tig0019271:141106-141125 None:intergenic 25.0%
!! CAAAAAAGATGTAGCTTTTT+CGG - tig0019271:141221-141240 Msa1372210:CDS 25.0%
!! TTTTTCATCATTACCTTCTT+GGG + tig0019271:141430-141449 None:intergenic 25.0%
!!! TTTTTTCATCATTACCTTCT+TGG + tig0019271:141431-141450 None:intergenic 25.0%
!! TCAATGTTGATTGTGATATT+TGG + tig0019271:142152-142171 None:intergenic 25.0%
! CAGCAAATATACTATAACAA+AGG + tig0019271:142185-142204 None:intergenic 25.0%
!!! TTGTTTTGATTGTTGTAGAA+TGG - tig0019271:142248-142267 Msa1372210:CDS 25.0%
! TAAACAATCAAAATACTCAC+CGG + tig0019271:142515-142534 None:intergenic 25.0%
GTATCACTATGTACATCAAA+AGG + tig0019271:139449-139468 None:intergenic 30.0%
TATCTTCAGTATCACTATCA+GGG + tig0019271:139571-139590 None:intergenic 30.0%
ATATCTTCAGTATCACTATC+AGG + tig0019271:139572-139591 None:intergenic 30.0%
ATGTGACAGATGATGATATA+AGG - tig0019271:139717-139736 Msa1372210:CDS 30.0%
TGTGACAGATGATGATATAA+GGG - tig0019271:139718-139737 Msa1372210:CDS 30.0%
AGATGATGATATAAGGGATA+TGG - tig0019271:139724-139743 Msa1372210:CDS 30.0%
GTATTGTTTGCAAAATACCA+CGG - tig0019271:139782-139801 Msa1372210:CDS 30.0%
TATTGTTTGCAAAATACCAC+GGG - tig0019271:139783-139802 Msa1372210:CDS 30.0%
TCAATTAGTATGCAATAAGG+AGG - tig0019271:139862-139881 Msa1372210:intron 30.0%
CAATTAGTATGCAATAAGGA+GGG - tig0019271:139863-139882 Msa1372210:intron 30.0%
TCACTCAATTTACGGTAATT+AGG + tig0019271:140064-140083 None:intergenic 30.0%
TGTAAGAACATGTCATATCT+TGG - tig0019271:140123-140142 Msa1372210:intron 30.0%
GTAAGAACATGTCATATCTT+GGG - tig0019271:140124-140143 Msa1372210:intron 30.0%
!!! TCATATCTTGGGTTTTTTGA+TGG - tig0019271:140135-140154 Msa1372210:intron 30.0%
!!! CATATCTTGGGTTTTTTGAT+GGG - tig0019271:140136-140155 Msa1372210:intron 30.0%
ATATAATTTCACGGATCATC+AGG - tig0019271:140204-140223 Msa1372210:intron 30.0%
CAAGGGTTAAAATACAAGAT+GGG - tig0019271:140227-140246 Msa1372210:intron 30.0%
AAGGGTTAAAATACAAGATG+GGG - tig0019271:140228-140247 Msa1372210:intron 30.0%
GAGTTTACATTTACAGATGA+TGG - tig0019271:140313-140332 Msa1372210:CDS 30.0%
!!! ACGCCTAGAAAACATTTTTT+GGG - tig0019271:140336-140355 Msa1372210:CDS 30.0%
ATACTTGTTCTTTCTATAGG+TGG + tig0019271:140419-140438 None:intergenic 30.0%
ACCTATAGAAAGAACAAGTA+TGG - tig0019271:140418-140437 Msa1372210:CDS 30.0%
TCCATACTTGTTCTTTCTAT+AGG + tig0019271:140422-140441 None:intergenic 30.0%
TATCCACTAAAAATGACAAG+GGG + tig0019271:140448-140467 None:intergenic 30.0%
AAACAATAGAGACATACAAG+TGG - tig0019271:140518-140537 Msa1372210:CDS 30.0%
AACAATAGAGACATACAAGT+GGG - tig0019271:140519-140538 Msa1372210:CDS 30.0%
! TTTTGGATGTTTGTCATACA+TGG + tig0019271:140566-140585 None:intergenic 30.0%
!! ATTTTGACCTATCAAACAAC+AGG - tig0019271:140794-140813 Msa1372210:CDS 30.0%
TTCTAACACCACACAAAAAA+TGG + tig0019271:140876-140895 None:intergenic 30.0%
AATTATGCAAGAAATCCACA+AGG + tig0019271:140960-140979 None:intergenic 30.0%
!!! TGGAAACATTGTCATTTTCA+AGG - tig0019271:141185-141204 Msa1372210:CDS 30.0%
!! TAAATTTGTTGCGAGTGATA+AGG - tig0019271:141617-141636 Msa1372210:CDS 30.0%
ATCATTCGCAACAAATTTGT+TGG + tig0019271:141643-141662 None:intergenic 30.0%
TGTTCTAATTGTAGAAATCC+TGG - tig0019271:141726-141745 Msa1372210:CDS 30.0%
GTTCTAATTGTAGAAATCCT+GGG - tig0019271:141727-141746 Msa1372210:CDS 30.0%
GATTCATTATCAGACTCAAT+AGG + tig0019271:141825-141844 None:intergenic 30.0%
AACAAATACAAGCACTAGAA+AGG - tig0019271:142109-142128 Msa1372210:CDS 30.0%
ACAAATACAAGCACTAGAAA+GGG - tig0019271:142110-142129 Msa1372210:CDS 30.0%
! ATTTTTCATGTCCAAAGTCA+CGG + tig0019271:142280-142299 None:intergenic 30.0%
AAAACTGATCCACCATTATT+TGG + tig0019271:142389-142408 None:intergenic 30.0%
AGTGTTAGAATGTGATGATA+GGG + tig0019271:142435-142454 None:intergenic 30.0%
TGTACATAGTGATACAGATG+AGG - tig0019271:139454-139473 Msa1372210:CDS 35.0%
! ACACTGATAGTGATACAGAT+GGG - tig0019271:139504-139523 Msa1372210:intron 35.0%
GTGGAATTTAACCATGGTAA+TGG - tig0019271:139641-139660 Msa1372210:CDS 35.0%
ATGTGAAGCGTGATTCTAAT+GGG - tig0019271:139825-139844 Msa1372210:intron 35.0%
GCGTCAATTAGTATGCAATA+AGG - tig0019271:139859-139878 Msa1372210:intron 35.0%
!! AATAAGGAGGGTGTTAATGA+TGG - tig0019271:139875-139894 Msa1372210:intron 35.0%
ATCAAATGGACAAAACGCAA+AGG + tig0019271:140040-140059 None:intergenic 35.0%
TACGGTAATTAGGCATCAAA+TGG + tig0019271:140054-140073 None:intergenic 35.0%
! GTTGATAAACAAGTTGCTAG+TGG - tig0019271:140085-140104 Msa1372210:CDS 35.0%
!!! GGTTTTTTGATGGGTCAAAA+GGG - tig0019271:140145-140164 Msa1372210:intron 35.0%
AGGACTTGGGTTTATCAAAA+AGG - tig0019271:140177-140196 Msa1372210:intron 35.0%
GCAAGGGTTAAAATACAAGA+TGG - tig0019271:140226-140245 Msa1372210:intron 35.0%
! TTTGAGTTACTTCCAAGGTA+AGG - tig0019271:140264-140283 Msa1372210:CDS 35.0%
!!! GACGCCTAGAAAACATTTTT+TGG - tig0019271:140335-140354 Msa1372210:CDS 35.0%
! CTGCCCAAAAAATGTTTTCT+AGG + tig0019271:140342-140361 None:intergenic 35.0%
! AGGTTTGACTATGAGTGTTT+TGG - tig0019271:140373-140392 Msa1372210:CDS 35.0%
!!! AAACCCCTTGTCATTTTTAG+TGG - tig0019271:140442-140461 Msa1372210:CDS 35.0%
! TTTAGTGGATATAACCACCA+TGG - tig0019271:140457-140476 Msa1372210:CDS 35.0%
!! CAAAAATGGTTGTTTCTCCA+TGG + tig0019271:140477-140496 None:intergenic 35.0%
GTATGACAAACATCCAAAAG+CGG - tig0019271:140567-140586 Msa1372210:CDS 35.0%
GAGTTGTGTTTCCAAATACA+AGG - tig0019271:140629-140648 Msa1372210:CDS 35.0%
!!! TCAAACTTTTCAGGTGTACA+AGG + tig0019271:140748-140767 None:intergenic 35.0%
! CACTCAGATTCAAACTTTTC+AGG + tig0019271:140757-140776 None:intergenic 35.0%
CTGAAAAGTTTGAATCTGAG+TGG - tig0019271:140755-140774 Msa1372210:CDS 35.0%
! TTTATCGGTTGAAGAGAATG+TGG - tig0019271:140830-140849 Msa1372210:CDS 35.0%
! TTATCGGTTGAAGAGAATGT+GGG - tig0019271:140831-140850 Msa1372210:CDS 35.0%
GAAGCTTCAAAGATATTCAC+CGG - tig0019271:141084-141103 Msa1372210:CDS 35.0%
ATTTAGTCTGCAACTTGTCA+AGG + tig0019271:141251-141270 None:intergenic 35.0%
CTTCATGGCACAAATAATGT+GGG + tig0019271:141319-141338 None:intergenic 35.0%
ACTTCATGGCACAAATAATG+TGG + tig0019271:141320-141339 None:intergenic 35.0%
ATCCATGTTGTCATACTTCA+TGG + tig0019271:141334-141353 None:intergenic 35.0%
! AAAGTCTGATTTGCAAGAGA+TGG - tig0019271:141364-141383 Msa1372210:CDS 35.0%
! TAAGAGCGCAAGTGATTATA+AGG - tig0019271:141512-141531 Msa1372210:CDS 35.0%
!! ATTATAAGGAGGCTCTAGAA+GGG - tig0019271:141526-141545 Msa1372210:CDS 35.0%
TAAGATAAATAGCCGTCATG+AGG - tig0019271:141566-141585 Msa1372210:CDS 35.0%
AAGATAAATAGCCGTCATGA+GGG - tig0019271:141567-141586 Msa1372210:CDS 35.0%
ATCACTCGCAACAAATTTAC+TGG + tig0019271:141616-141635 None:intergenic 35.0%
!!! TGTTGACTTTTTACTCCTAC+GGG + tig0019271:141691-141710 None:intergenic 35.0%
!!! GTGTTGACTTTTTACTCCTA+CGG + tig0019271:141692-141711 None:intergenic 35.0%
TTAGAACAACATCTTGTCCT+TGG + tig0019271:141714-141733 None:intergenic 35.0%
TATCATTACTCATAGACAGC+TGG + tig0019271:141953-141972 None:intergenic 35.0%
!! TGCTGAAAATTGTGTTGCTT+TGG - tig0019271:142200-142219 Msa1372210:CDS 35.0%
! TTTTTCATGTCCAAAGTCAC+GGG + tig0019271:142279-142298 None:intergenic 35.0%
! CAAGTTGGAACCATAATGTT+GGG - tig0019271:142327-142346 Msa1372210:CDS 35.0%
TAACACCATACCCAACATTA+TGG + tig0019271:142340-142359 None:intergenic 35.0%
CAGTGTTAGAATGTGATGAT+AGG + tig0019271:142436-142455 None:intergenic 35.0%
ACATAGTGATACAGATGAGG+TGG - tig0019271:139457-139476 Msa1372210:CDS 40.0%
! GACACTGATAGTGATACAGA+TGG - tig0019271:139503-139522 Msa1372210:intron 40.0%
! CACTGATAGTGATACAGATG+GGG - tig0019271:139505-139524 Msa1372210:intron 40.0%
AACAACGATGTTCGTACTGA+TGG - tig0019271:139605-139624 Msa1372210:CDS 40.0%
CATTGCAAACACCATTACCA+TGG + tig0019271:139655-139674 None:intergenic 40.0%
AATGGTGTTTGCAATGAGCA+AGG - tig0019271:139659-139678 Msa1372210:CDS 40.0%
ATGGTGTTTGCAATGAGCAA+GGG - tig0019271:139660-139679 Msa1372210:CDS 40.0%
CAAGGGAAAGAGAACTTTGA+TGG - tig0019271:139677-139696 Msa1372210:CDS 40.0%
GGATATGGAATTCAACACAG+AGG - tig0019271:139739-139758 Msa1372210:CDS 40.0%
GATGTGAAGCGTGATTCTAA+TGG - tig0019271:139824-139843 Msa1372210:intron 40.0%
TTCGAATGGTTAGCGTAGAT+CGG + tig0019271:139922-139941 None:intergenic 40.0%
CGCAGAACAATTAGTTCGAA+TGG + tig0019271:139936-139955 None:intergenic 40.0%
ACGACTTTGTTCGTACGATT+TGG - tig0019271:139972-139991 Msa1372210:CDS 40.0%
GCTAATTCATGGTTGTGAGA+GGG + tig0019271:140016-140035 None:intergenic 40.0%
AGCTAATTCATGGTTGTGAG+AGG + tig0019271:140017-140036 None:intergenic 40.0%
AACGCAAAGGAGCTAATTCA+TGG + tig0019271:140027-140046 None:intergenic 40.0%
!!! GGGTTTTTTGATGGGTCAAA+AGG - tig0019271:140144-140163 Msa1372210:intron 40.0%
!! TTTTTGATGGGTCAAAAGGG+TGG - tig0019271:140148-140167 Msa1372210:intron 40.0%
TGATAAACCCAAGTCCTTCA+TGG + tig0019271:140174-140193 None:intergenic 40.0%
! GCTACTTTGAGTTACTTCCA+AGG - tig0019271:140259-140278 Msa1372210:CDS 40.0%
TATCAGTATCAGCCTTACCT+TGG + tig0019271:140279-140298 None:intergenic 40.0%
TTTGGGCAGATTCTACTAGT+AGG - tig0019271:140353-140372 Msa1372210:CDS 40.0%
!! AAATGGTTGTTTCTCCATGG+TGG + tig0019271:140474-140493 None:intergenic 40.0%
CAAAAGCGGTGATTACTGAT+GGG - tig0019271:140581-140600 Msa1372210:CDS 40.0%
AAAAGCGGTGATTACTGATG+GGG - tig0019271:140582-140601 Msa1372210:CDS 40.0%
AGTTTGAATCTGAGTGGAAG+AGG - tig0019271:140761-140780 Msa1372210:CDS 40.0%
GTTTGAATCTGAGTGGAAGA+GGG - tig0019271:140762-140781 Msa1372210:CDS 40.0%
TTGACCTATCAAACAACAGG+TGG - tig0019271:140797-140816 Msa1372210:CDS 40.0%
TGACCTATCAAACAACAGGT+GGG - tig0019271:140798-140817 Msa1372210:CDS 40.0%
! TTACCCACCTGTTGTTTGAT+AGG + tig0019271:140804-140823 None:intergenic 40.0%
!!! ATGCGAGACCATTTTTTGTG+TGG - tig0019271:140865-140884 Msa1372210:CDS 40.0%
AGAACGACATCAATCTGTGA+AGG - tig0019271:140892-140911 Msa1372210:CDS 40.0%
AGATGTCAAAAAGGCGATAG+AGG - tig0019271:141119-141138 Msa1372210:CDS 40.0%
CATTGAACGAGATGAAGATG+AGG - tig0019271:141161-141180 Msa1372210:CDS 40.0%
GCACAAATAATGTGGGAACA+TGG + tig0019271:141312-141331 None:intergenic 40.0%
TGCCATGAAGTATGACAACA+TGG - tig0019271:141329-141348 Msa1372210:CDS 40.0%
CTCTTGCAAATCAGACTTTC+AGG + tig0019271:141363-141382 None:intergenic 40.0%
TTCATCATTACCTTCTTGGG+AGG + tig0019271:141427-141446 None:intergenic 40.0%
AAAATGACCATGTTTCGTCG+AGG - tig0019271:141447-141466 Msa1372210:CDS 40.0%
AAATGACCATGTTTCGTCGA+GGG - tig0019271:141448-141467 Msa1372210:CDS 40.0%
ACATAGACTATTGAACGCAG+CGG + tig0019271:141481-141500 None:intergenic 40.0%
!! GATTATAAGGAGGCTCTAGA+AGG - tig0019271:141525-141544 Msa1372210:CDS 40.0%
AAATGGACATGACGAGGATA+AGG - tig0019271:141590-141609 Msa1372210:CDS 40.0%
GGAGTAAAAAGTCAACACCA+AGG - tig0019271:141694-141713 Msa1372210:CDS 40.0%
CACTGGTATTACACGTAAGA+GGG - tig0019271:142031-142050 Msa1372210:CDS 40.0%
TATGGTTCCAACTTGCACAA+TGG + tig0019271:142322-142341 None:intergenic 40.0%
! GCAAGTTGGAACCATAATGT+TGG - tig0019271:142326-142345 Msa1372210:CDS 40.0%
TGGAACCATAATGTTGGGTA+TGG - tig0019271:142332-142351 Msa1372210:CDS 40.0%
TGTGATGATAGGGTAGTTGT+CGG + tig0019271:142425-142444 None:intergenic 40.0%
CAATGTTCCGAGTTATACAC+CGG - tig0019271:142493-142512 Msa1372210:CDS 40.0%
ATACTCACCGGTGTATAACT+CGG + tig0019271:142503-142522 None:intergenic 40.0%
TATGAGTGCGTTGAAGAATG+TGG - tig0019271:142603-142622 Msa1372210:CDS 40.0%
AAACATCGCAGTGACTATTG+TGG - tig0019271:142637-142656 Msa1372210:CDS 40.0%
! TGATAGTGATACAGATGGGG+TGG - tig0019271:139508-139527 Msa1372210:intron 45.0%
GACGGTGTGGAATTTAACCA+TGG - tig0019271:139635-139654 Msa1372210:CDS 45.0%
CCTTTCTTACTTCAAACCCG+TGG + tig0019271:139802-139821 None:intergenic 45.0%
! CCACGGGTTTGAAGTAAGAA+AGG - tig0019271:139799-139818 Msa1372210:CDS 45.0%
ATTTCACGGATCATCAGGCA+AGG - tig0019271:140209-140228 Msa1372210:intron 45.0%
TTTCACGGATCATCAGGCAA+GGG - tig0019271:140210-140229 Msa1372210:intron 45.0%
! CCATCAGTAATCACCGCTTT+TGG + tig0019271:140583-140602 None:intergenic 45.0%
CCAAAAGCGGTGATTACTGA+TGG - tig0019271:140580-140599 Msa1372210:CDS 45.0%
CAAAGGCGATGCCTTGTATT+TGG + tig0019271:140643-140662 None:intergenic 45.0%
AGCATTTCGATGCAAATGCC+AGG + tig0019271:140668-140687 None:intergenic 45.0%
!!! CCACAAGGCTGTTTTTGTGT+CGG + tig0019271:140945-140964 None:intergenic 45.0%
GTCAATGCAGTTGTCAGCAT+AGG + tig0019271:141051-141070 None:intergenic 45.0%
GAACGAGATGAAGATGAGGA+TGG - tig0019271:141165-141184 Msa1372210:CDS 45.0%
! TGTAGACGCTTTGAACGTAG+AGG - tig0019271:141282-141301 Msa1372210:CDS 45.0%
AATGACCATGTTTCGTCGAG+GGG - tig0019271:141449-141468 Msa1372210:CDS 45.0%
GAGCGCAAGTGATTATAAGG+AGG - tig0019271:141515-141534 Msa1372210:CDS 45.0%
AATAGCCGTCATGAGGGAAA+TGG - tig0019271:141573-141592 Msa1372210:CDS 45.0%
CATGTCCATTTCCCTCATGA+CGG + tig0019271:141581-141600 None:intergenic 45.0%
TGTTGCGAATGATCCACTGA+TGG - tig0019271:141650-141669 Msa1372210:CDS 45.0%
CCTTTGACATTCACCATCAG+TGG + tig0019271:141666-141685 None:intergenic 45.0%
! CCACTGATGGTGAATGTCAA+AGG - tig0019271:141663-141682 Msa1372210:CDS 45.0%
GAAATCCTGGGCACACAATA+CGG - tig0019271:141739-141758 Msa1372210:CDS 45.0%
!! TGTCCAGAACTTGCTGGAAT+TGG - tig0019271:141765-141784 Msa1372210:CDS 45.0%
ACACCAATTCCAGCAAGTTC+TGG + tig0019271:141771-141790 None:intergenic 45.0%
GTGTCGTGTCTGAAGATCAA+GGG - tig0019271:141787-141806 Msa1372210:CDS 45.0%
TGTCGTGTCTGAAGATCAAG+GGG - tig0019271:141788-141807 Msa1372210:CDS 45.0%
CATTTGACACTGCTACAGAG+TGG + tig0019271:141989-142008 None:intergenic 45.0%
CACTCTGTAGCAGTGTCAAA+TGG - tig0019271:141987-142006 Msa1372210:CDS 45.0%
TCTGTAGCAGTGTCAAATGG+TGG - tig0019271:141990-142009 Msa1372210:CDS 45.0%
! CGAGATGTTGAGAAAAGCAC+TGG - tig0019271:142014-142033 Msa1372210:CDS 45.0%
! GCACTGGTATTACACGTAAG+AGG - tig0019271:142030-142049 Msa1372210:CDS 45.0%
AAGACAGCCATTGTGCAAGT+TGG - tig0019271:142312-142331 Msa1372210:CDS 45.0%
! CACTATGGGGTGCCAAATAA+TGG - tig0019271:142374-142393 Msa1372210:CDS 45.0%
! TATGGGGTGCCAAATAATGG+TGG - tig0019271:142377-142396 Msa1372210:CDS 45.0%
ATGATAGGGTAGTTGTCGGA+GGG + tig0019271:142421-142440 None:intergenic 45.0%
TCTAACACTGCTCTGCATAC+TGG - tig0019271:142446-142465 Msa1372210:CDS 45.0%
ACTGGAACACACAATGTTCC+TGG - tig0019271:142464-142483 Msa1372210:CDS 45.0%
! CGTACTGATGGAGATGCAGA+CGG - tig0019271:139617-139636 Msa1372210:CDS 50.0%
GCTAGTGGTCTACATTCGCA+AGG - tig0019271:140100-140119 Msa1372210:CDS 50.0%
CCGACACAAAAACAGCCTTG+TGG - tig0019271:140942-140961 Msa1372210:CDS 50.0%
TCGTCATTTGCCTCCCAAGA+AGG - tig0019271:141414-141433 Msa1372210:CDS 50.0%
TGAGGGAAATGGACATGACG+AGG - tig0019271:141584-141603 Msa1372210:CDS 50.0%
!! TGAATGTCAAAGGTGCCCGT+AGG - tig0019271:141673-141692 Msa1372210:CDS 50.0%
! GGTGTCGTGTCTGAAGATCA+AGG - tig0019271:141786-141805 Msa1372210:CDS 50.0%
GTCTGAAGATCAAGGGGAGA+AGG - tig0019271:141794-141813 Msa1372210:CDS 50.0%
AATGGTGTGACCCGTGACTT+TGG - tig0019271:142266-142285 Msa1372210:CDS 50.0%
GTAGTTGTCGGAGGGAATTG+TGG + tig0019271:142413-142432 None:intergenic 50.0%
GATGATAGGGTAGTTGTCGG+AGG + tig0019271:142422-142441 None:intergenic 50.0%
CTCGGAACATTGTGTGTTCC+AGG + tig0019271:142485-142504 None:intergenic 50.0%
TGATGGAGATGCAGACGGTG+TGG - tig0019271:139622-139641 Msa1372210:CDS 55.0%
GGTCAAAAGGGTGGCCATGA+AGG - tig0019271:140157-140176 Msa1372210:intron 55.0%
AAGGGTGGCCATGAAGGACT+TGG - tig0019271:140163-140182 Msa1372210:intron 55.0%
AGGGTGGCCATGAAGGACTT+GGG - tig0019271:140164-140183 Msa1372210:intron 55.0%
GATGCAAATGCCAGGCGCAA+AGG + tig0019271:140660-140679 None:intergenic 55.0%
AAGTGCCCCTCGACGAAACA+TGG + tig0019271:141457-141476 None:intergenic 55.0%
CACGTCCGTATTGTGTGCCC+AGG + tig0019271:141747-141766 None:intergenic 60.0%
CGGACGTGTCCAGAACTTGC+TGG - tig0019271:141759-141778 Msa1372210:CDS 60.0%
!! CAAGGCATCGCCTTTGCGCC+TGG - tig0019271:140647-140666 Msa1372210:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0019271 gene 139419 142672 139419 ID=Msa1372210;Name=Msa1372210
tig0019271 mRNA 139419 142672 139419 ID=Msa1372210-mRNA-1;Parent=Msa1372210;Name=Msa1372210-mRNA-1;_AED=0.25;_eAED=0.26;_QI=0|0|0|0.25|1|1|4|0|993
tig0019271 exon 140234 142672 140234 ID=Msa1372210-mRNA-1:exon:2651;Parent=Msa1372210-mRNA-1
tig0019271 exon 139916 140137 139916 ID=Msa1372210-mRNA-1:exon:2650;Parent=Msa1372210-mRNA-1
tig0019271 exon 139577 139825 139577 ID=Msa1372210-mRNA-1:exon:2649;Parent=Msa1372210-mRNA-1
tig0019271 exon 139419 139490 139419 ID=Msa1372210-mRNA-1:exon:2648;Parent=Msa1372210-mRNA-1
tig0019271 CDS 140234 142672 140234 ID=Msa1372210-mRNA-1:cds;Parent=Msa1372210-mRNA-1
tig0019271 CDS 139916 140137 139916 ID=Msa1372210-mRNA-1:cds;Parent=Msa1372210-mRNA-1
tig0019271 CDS 139577 139825 139577 ID=Msa1372210-mRNA-1:cds;Parent=Msa1372210-mRNA-1
tig0019271 CDS 139419 139490 139419 ID=Msa1372210-mRNA-1:cds;Parent=Msa1372210-mRNA-1
Gene Sequence

>Msa1372210

ATGTCTACATATAGTGAAGCAACATTGCCTTTTGATGTACATAGTGATACAGATGAGGTGGAATTTAATAGTGTTAATGATATTGACACTGATAGTGATACAGATGGGGTGGAATTTAATAGTGTTAATGATGTTGACACTGATAGTGACCCTGATAGTGATACTGAAGATATAAAAGTCAATAGCAACAACGATGTTCGTACTGATGGAGATGCAGACGGTGTGGAATTTAACCATGGTAATGGTGTTTGCAATGAGCAAGGGAAAGAGAACTTTGATGGCTACAAATGCATCAGTGATGTGACAGATGATGATATAAGGGATATGGAATTCAACACAGAGGATGAAGCTATAAAGTTTTATGTATTGTTTGCAAAATACCACGGGTTTGAAGTAAGAAAGGATGATGTGAAGCGTGATTCTAATGGGAACATTATTATGCGTCAATTAGTATGCAATAAGGAGGGTGTTAATGATGGAAAAAATAAGAAGAATGGCAGCCGATCTACGCTAACCATTCGAACTAATTGTTCTGCGAAACTTCGAGTAGTCTACGACTTTGTTCGTACGATTTGGAGAGTTTCTATTTTCAAACCCTCTCACAACCATGAATTAGCTCCTTTGCGTTTTGTCCATTTGATGCCTAATTACCGTAAATTGAGTGAAGTTGATAAACAAGTTGCTAGTGGTCTACATTCGCAAGGTGTAAGAACATGTCATATCTTGGGTTTTTTGATGGGTCAAAAGGGTGGCCATGAAGGACTTGGGTTTATCAAAAAGGACTTATATAATTTCACGGATCATCAGGCAAGGGTTAAAATACAAGATGGGGATACTTTTGCTACTTTGAGTTACTTCCAAGGTAAGGCTGATACTGATACATCATTCTTTTCAGAGTTTACATTTACAGATGATGGACGCCTAGAAAACATTTTTTGGGCAGATTCTACTAGTAGGTTTGACTATGAGTGTTTTGGCGATGTTGTTGCATTTGACACCACCTATAGAAAGAACAAGTATGGAAAACCCCTTGTCATTTTTAGTGGATATAACCACCATGGAGAAACAACCATTTTTGCTTGTGCTTTAATTTGTGATGAAACAATAGAGACATACAAGTGGGTTCTAAATTTGTTTTCAAAGGCCATGTATGACAAACATCCAAAAGCGGTGATTACTGATGGGGATAAATCAATGAGAAAAGCTATCAGAGTTGTGTTTCCAAATACAAGGCATCGCCTTTGCGCCTGGCATTTGCATCGAAATGCTGTTCAACATGTGAAGAATTCAAAAATTTTAGAAGAGTTCAAGTCACTGATGTATTCTCCTTGTACACCTGAAAAGTTTGAATCTGAGTGGAAGAGGGTTGTTGAAAATTTTGACCTATCAAACAACAGGTGGGTAAAAAAAATTTATCGGTTGAAGAGAATGTGGGCAAGTGCATACATGCGAGACCATTTTTTGTGTGGTGTTAGAACGACATCAATCTGTGAAGGAGTGAATTCATTTATCAAGAAGTATGTCCGACACAAAAACAGCCTTGTGGATTTCTTGCATAATTTTGAAAGAGCTCTCAAAGAATACAGACATAATGAATTATTGTCTGATTTCAAGTCGTTCTATTATCATCCTATGCTGACAACTGCATTGACTACTTTTGAGTCTGAAGCTTCAAAGATATTCACCGGTAAAAAGTTTAAAGATGTCAAAAAGGCGATAGAGGATGTTGTTGCATTATCAGTCATTGAACGAGATGAAGATGAGGATGGAAACATTGTCATTTTCAAGGTTAATGTATTTGGCAAAAAAGATGTAGCTTTTTCGGTATACCTTGACAAGTTGCAGACTAAATTTTTATGTGATTGTAGACGCTTTGAACGTAGAGGACTGCCATGTTCCCACATTATTTGTGCCATGAAGTATGACAACATGGATGTGTTTCCTGAAAGTCTGATTTGCAAGAGATGGACAAAGACTGCAAAGAGTGATCACATCTCGTCATTTGCCTCCCAAGAAGGTAATGATGAAAAAATGACCATGTTTCGTCGAGGGGCACTTGCCGCTGCGTTCAATAGTCTATGTGATGTTTCGTGTAAGAGCGCAAGTGATTATAAGGAGGCTCTAGAAGGGATTTATAGTCTGTGTAATAAGATAAATAGCCGTCATGAGGGAAATGGACATGACGAGGATAAGGCCAGTAAATTTGTTGCGAGTGATAAGGCCAACAAATTTGTTGCGAATGATCCACTGATGGTGAATGTCAAAGGTGCCCGTAGGAGTAAAAAGTCAACACCAAGGACAAGATGTTGTTCTAATTGTAGAAATCCTGGGCACACAATACGGACGTGTCCAGAACTTGCTGGAATTGGTGTCGTGTCTGAAGATCAAGGGGAGAAGGCTTCACCTATTGAGTCTGATAATGAATCAAAAAGTTTAAGTCTGTCTATGAGTAATGATATGAATGATGACATCCACTCTGTAGCAGTGTCAAATGGTGGACGAGATGTTGAGAAAAGCACTGGTATTACACGTAAGAGGGAATGTGACAATGTTGCATTTTCAACACAAGACTCTGTCAACAAAAGAAAAACAAAAACAAATACAAGCACTAGAAAGGGTGATGCTTGTGTGACTCCAAATATCACAATCAACATTGATAATAATGGTGTGACCCGTGACTTTGGACATGAAAAATCTACTCTCAAACAAGACAGCCATTGTGCAAGTTGGAACCATAATGTTGGGTATGGTGTTAATTATTTTAATATGCACTATGGGGTGCCAAATAATGGTGGATCAGTTTTTCCACAATTCCCTCCGACAACTACCCTATCATCACATTCTAACACTGCTCTGCATACTGGAACACACAATGTTCCTGGAACACACAATGTTCCGAGTTATACACCGTTTATGAGTGCGTTGAAGAATGTGGAGCTGTTAGCAAAACATCGCAGTGACTATTGTGGACCAAAACATTGA
Protein sequence

>Msa1372210

MSTYSEATLPFDVHSDTDEVEFNSVNDIDTDSDTDGVEFNSVNDVDTDSDPDSDTEDIKVNSNNDVRTDGDADGVEFNHGNGVCNEQGKENFDGYKCISDVTDDDIRDMEFNTEDEAIKFYVLFAKYHGFEVRKDDVKRDSNGNIIMRQLVCNKEGVNDGKNKKNGSRSTLTIRTNCSAKLRVVYDFVRTIWRVSIFKPSHNHELAPLRFVHLMPNYRKLSEVDKQVASGLHSQGVRTCHILGFLMGQKGGHEGLGFIKKDLYNFTDHQARVKIQDGDTFATLSYFQGKADTDTSFFSEFTFTDDGRLENIFWADSTSRFDYECFGDVVAFDTTYRKNKYGKPLVIFSGYNHHGETTIFACALICDETIETYKWVLNLFSKAMYDKHPKAVITDGDKSMRKAIRVVFPNTRHRLCAWHLHRNAVQHVKNSKILEEFKSLMYSPCTPEKFESEWKRVVENFDLSNNRWVKKIYRLKRMWASAYMRDHFLCGVRTTSICEGVNSFIKKYVRHKNSLVDFLHNFERALKEYRHNELLSDFKSFYYHPMLTTALTTFESEASKIFTGKKFKDVKKAIEDVVALSVIERDEDEDGNIVIFKVNVFGKKDVAFSVYLDKLQTKFLCDCRRFERRGLPCSHIICAMKYDNMDVFPESLICKRWTKTAKSDHISSFASQEGNDEKMTMFRRGALAAAFNSLCDVSCKSASDYKEALEGIYSLCNKINSRHEGNGHDEDKASKFVASDKANKFVANDPLMVNVKGARRSKKSTPRTRCCSNCRNPGHTIRTCPELAGIGVVSEDQGEKASPIESDNESKSLSLSMSNDMNDDIHSVAVSNGGRDVEKSTGITRKRECDNVAFSTQDSVNKRKTKTNTSTRKGDACVTPNITINIDNNGVTRDFGHEKSTLKQDSHCASWNHNVGYGVNYFNMHYGVPNNGGSVFPQFPPTTTLSSHSNTALHTGTHNVPGTHNVPSYTPFMSALKNVELLAKHRSDYCGPKH*