AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1440090

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1440090 MtrunA17_Chr2g0287651 42.960 696 378 6 93 783 63 744 0.0 586
Msa1440090 MtrunA17_Chr1g0203051 34.564 596 367 6 68 648 34 621 4.72e-117 373
Msa1440090 MtrunA17_Chr1g0202341 32.709 587 372 10 104 676 50 627 1.02e-96 320
Msa1440090 MtrunA17_Chr5g0448761 32.632 570 363 9 109 663 64 627 1.15e-93 315
Msa1440090 MtrunA17_Chr2g0324011 29.181 586 397 6 109 682 14 593 1.47e-85 287
Msa1440090 MtrunA17_Chr2g0296781 29.204 678 433 15 109 764 1 653 3.61e-84 287
Msa1440090 MtrunA17_Chr8g0363841 28.081 641 424 11 101 716 9 637 1.10e-83 284
Msa1440090 MtrunA17_Chr5g0441581 28.346 635 408 12 109 724 36 642 1.89e-82 281
Msa1440090 MtrunA17_Chr1g0198761 31.196 577 366 11 111 665 53 620 2.34e-80 277
Msa1440090 MtrunA17_Chr3g0082121 30.099 608 396 12 108 704 46 635 1.95e-79 273
Msa1440090 MtrunA17_Chr6g0477261 34.813 428 264 6 245 661 22 445 2.05e-78 270
Msa1440090 MtrunA17_Chr1g0203721 27.885 624 429 11 111 724 69 681 2.91e-76 268
Msa1440090 MtrunA17_Chr1g0207551 30.838 561 371 9 109 661 38 589 2.12e-74 262
Msa1440090 MtrunA17_Chr6g0458391 28.042 567 364 11 109 661 229 765 2.51e-72 255
Msa1440090 MtrunA17_Chr3g0080351 27.885 624 383 13 100 699 71 651 2.03e-71 254
Msa1440090 MtrunA17_Chr1g0196541 30.594 572 362 15 109 661 1 556 5.60e-70 248
Msa1440090 MtrunA17_Chr2g0329031 26.094 617 409 11 92 694 1 584 4.22e-69 245
Msa1440090 MtrunA17_Chr5g0440451 27.244 624 406 15 109 709 47 645 7.84e-65 236
Msa1440090 MtrunA17_Chr3g0118611 29.862 509 325 11 211 709 56 542 1.43e-64 233
Msa1440090 MtrunA17_Chr5g0448511 30.653 398 266 5 271 662 5 398 1.65e-64 228
Msa1440090 MtrunA17_Chr5g0431001 32.151 423 261 8 111 512 67 484 6.95e-63 222
Msa1440090 MtrunA17_Chr2g0276841 27.200 625 425 14 111 721 76 684 7.98e-63 226
Msa1440090 MtrunA17_Chr2g0290391 26.596 658 417 16 109 730 55 682 4.79e-61 221
Msa1440090 MtrunA17_Chr2g0291581 27.592 569 374 13 109 661 75 621 6.80e-60 218
Msa1440090 MtrunA17_Chr2g0287061 27.558 606 388 14 110 709 188 748 1.17e-58 216
Msa1440090 MtrunA17_Chr2g0291591 30.247 486 313 10 106 580 65 535 1.18e-57 209
Msa1440090 MtrunA17_Chr2g0294841 28.196 571 379 14 111 662 51 609 2.79e-57 211
Msa1440090 MtrunA17_Chr1g0164591 32.459 305 192 3 192 491 12 307 2.16e-50 181
Msa1440090 MtrunA17_Chr2g0308841 33.051 236 156 1 436 671 4 237 1.33e-38 145
Msa1440090 MtrunA17_Chr7g0258861 31.579 285 176 5 109 382 14 290 1.14e-37 143
Msa1440090 MtrunA17_Chr1g0149131 34.545 110 67 1 383 487 1 110 2.94e-16 76.3
Msa1440090 MtrunA17_Chr8g0340801 33.945 109 70 1 213 321 1 107 4.00e-14 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1440090 AT4G38180.1 31.424 646 420 12 33 665 3 638 1.92e-93 314
Msa1440090 AT4G15090.1 29.552 670 442 14 57 711 2 656 1.11e-84 291
Msa1440090 AT4G15090.5 29.552 670 442 14 57 711 2 656 1.11e-84 291
Msa1440090 AT4G15090.3 29.552 670 442 14 57 711 2 656 1.11e-84 291
Msa1440090 AT4G15090.2 29.552 670 442 14 57 711 2 656 1.11e-84 291
Msa1440090 AT4G15090.4 29.552 670 442 14 57 711 2 656 1.11e-84 291
Msa1440090 AT3G22170.2 26.744 688 440 14 56 720 30 676 3.69e-72 256
Msa1440090 AT3G22170.1 26.744 688 440 14 56 720 30 676 3.69e-72 256
Msa1440090 AT2G27110.2 26.147 654 416 14 63 703 14 613 5.72e-67 242
Msa1440090 AT2G27110.1 26.147 654 416 14 63 703 14 613 5.72e-67 242
Msa1440090 AT3G06250.2 27.107 605 390 11 109 709 194 751 7.50e-64 231
Msa1440090 AT3G06250.1 27.107 605 390 11 109 709 194 751 7.50e-64 231
Msa1440090 AT3G06250.3 27.107 605 390 11 109 709 194 751 7.50e-64 231
Msa1440090 AT5G28530.1 28.250 577 382 11 111 667 61 625 1.10e-63 229
Msa1440090 AT1G10240.2 27.087 635 413 13 111 721 54 662 7.40e-63 226
Msa1440090 AT1G10240.1 27.087 635 413 13 111 721 54 662 7.40e-63 226
Msa1440090 AT5G28530.3 28.076 577 379 12 111 667 61 621 2.33e-62 225
Msa1440090 AT5G28530.2 28.076 577 379 12 111 667 61 621 2.33e-62 225
Msa1440090 AT2G27110.3 30.324 432 281 7 278 703 51 468 3.93e-62 225
Msa1440090 AT1G76320.2 26.876 573 376 13 109 664 1 547 9.36e-62 224
Msa1440090 AT1G76320.1 26.876 573 376 13 109 664 1 547 9.56e-62 224
Msa1440090 AT1G76320.4 26.876 573 376 13 109 664 1 547 9.56e-62 224
Msa1440090 AT1G76320.3 26.876 573 376 13 109 664 1 547 1.41e-61 223
Msa1440090 AT4G19990.3 27.903 577 356 13 110 665 26 563 1.72e-61 224
Msa1440090 AT4G19990.4 27.903 577 356 13 110 665 26 563 1.72e-61 224
Msa1440090 AT4G19990.6 27.556 577 358 12 110 665 26 563 1.73e-61 224
Msa1440090 AT4G19990.2 27.556 577 358 12 110 665 26 563 1.73e-61 224
Msa1440090 AT4G19990.7 27.778 612 373 15 83 665 6 577 1.89e-61 224
Msa1440090 AT4G19990.5 27.903 577 356 13 110 665 26 563 2.21e-61 224
Msa1440090 AT1G80010.1 28.155 618 387 20 84 670 79 670 2.34e-61 223
Msa1440090 AT1G52520.1 27.604 576 378 13 109 666 89 643 2.41e-61 223
Msa1440090 AT5G18960.2 26.733 606 394 13 109 709 215 775 9.12e-60 219
Msa1440090 AT5G18960.1 26.733 606 394 13 109 709 215 775 9.12e-60 219
Msa1440090 AT4G19990.1 29.825 399 272 4 273 665 110 506 3.58e-59 216
Msa1440090 AT2G32250.2 26.904 617 365 14 109 701 42 596 3.97e-56 209
Msa1440090 AT2G32250.4 26.904 617 365 14 109 701 42 596 3.97e-56 209
Msa1440090 AT2G32250.8 26.742 617 366 14 109 701 1 555 4.12e-56 208
Msa1440090 AT2G32250.7 26.742 617 366 14 109 701 1 555 5.14e-56 208
Msa1440090 AT2G32250.6 26.904 617 365 14 109 701 42 596 5.22e-56 209
Msa1440090 AT2G32250.1 26.904 617 365 14 109 701 42 596 5.22e-56 209
Msa1440090 AT2G32250.3 26.904 617 365 14 109 701 42 596 5.22e-56 209
Msa1440090 AT2G32250.5 26.904 617 365 14 109 701 42 596 6.66e-56 208
Msa1440090 AT4G38170.1 28.337 427 277 9 310 720 45 458 6.51e-55 201
Msa1440090 AT5G28530.4 25.220 567 349 14 111 667 61 562 6.90e-43 167
Msa1440090 AT5G28530.5 24.868 567 347 14 111 667 61 558 7.74e-42 163

Find 173 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAACTGATCCACCATTATT+TGG 0.155571 tig0028948:-35965 None:intergenic
CAAAGGCGATGCCTTGTATT+TGG 0.159430 tig0028948:-34219 None:intergenic
TCAATGTTGATTGTGATATT+TGG 0.171428 tig0028948:-35728 None:intergenic
CTCTTGCAAATCAGACTTTC+AGG 0.192125 tig0028948:-34939 None:intergenic
TCACTCAATTTACGGTAATT+AGG 0.235603 tig0028948:-33640 None:intergenic
CCCATAGTTTCTCAATGTTT+TGG 0.242135 tig0028948:-36240 None:intergenic
TATCAACTTCACTCAATTTA+CGG 0.276736 tig0028948:-33648 None:intergenic
ATCACTCGCAACAAATTTAC+TGG 0.280329 tig0028948:-35192 None:intergenic
CCATCAGTAATCACCGCTTT+TGG 0.302214 tig0028948:-34159 None:intergenic
TCCATACTTGTTCTTTCTAT+AGG 0.305560 tig0028948:-33998 None:intergenic
TAACACCATACCCAACATTA+TGG 0.313783 tig0028948:-35916 None:intergenic
AGGACTTGGGTTTATCAAAA+AGG 0.326599 tig0028948:+33756 Msa1440090:CDS
CAGTGTTAGAATGTGATGAT+AGG 0.338987 tig0028948:-36012 None:intergenic
TGATAAACCCAAGTCCTTCA+TGG 0.346028 tig0028948:-33750 None:intergenic
AGATGATGATATAAGGGATA+TGG 0.347376 tig0028948:+33303 Msa1440090:CDS
ACCTATAGAAAGAACAAGTA+TGG 0.349144 tig0028948:+33997 Msa1440090:CDS
ACGACTTTGTTCGTACGATT+TGG 0.356788 tig0028948:+33551 Msa1440090:CDS
ACACCAATTCCAGCAAGTTC+TGG 0.360337 tig0028948:-35347 None:intergenic
ATGTGACAGATGATGATATA+AGG 0.367162 tig0028948:+33296 Msa1440090:CDS
GAGTTTACATTTACAGATGA+TGG 0.367609 tig0028948:+33892 Msa1440090:CDS
CTCGGAACATTGTGTGTTCC+AGG 0.370631 tig0028948:-36061 None:intergenic
TAAGAGCGCAAGTGATTATA+AGG 0.371814 tig0028948:+35091 Msa1440090:CDS
AGGTTTGACTATGAGTGTTT+TGG 0.377109 tig0028948:+33952 Msa1440090:CDS
TTACCCACCTGTTGTTTGAT+AGG 0.378005 tig0028948:-34380 None:intergenic
TGTAAGAACATGTCATATCT+TGG 0.387474 tig0028948:+33702 Msa1440090:CDS
TGTTCTAATTGTAGAAATCC+TGG 0.392101 tig0028948:+35305 Msa1440090:CDS
CACTATGGGGTGCCAAATAA+TGG 0.393131 tig0028948:+35953 Msa1440090:CDS
ATCCATGTTGTCATACTTCA+TGG 0.394339 tig0028948:-34910 None:intergenic
ACTGGAACACACAATGTTCC+TGG 0.398163 tig0028948:+36043 Msa1440090:CDS
ATCATTCGCAACAAATTTGT+TGG 0.403036 tig0028948:-35219 None:intergenic
TCTAACACTGCTCTGCATAC+TGG 0.407068 tig0028948:+36025 Msa1440090:CDS
ATATAATTTCACGGATCATC+AGG 0.409639 tig0028948:+33783 Msa1440090:CDS
TTTGGGCAGATTCTACTAGT+AGG 0.409763 tig0028948:+33932 Msa1440090:CDS
GATTATAAGGAGGCTCTAGA+AGG 0.413898 tig0028948:+35104 Msa1440090:CDS
GATTCATTATCAGACTCAAT+AGG 0.414121 tig0028948:-35401 None:intergenic
GCACTGGTATTACACGTAAG+AGG 0.418801 tig0028948:+35609 Msa1440090:CDS
CAAGGCATCGCCTTTGCGCC+TGG 0.420887 tig0028948:+34226 Msa1440090:CDS
AACAAATACAAGCACTAGAA+AGG 0.422563 tig0028948:+35688 Msa1440090:CDS
TGTCCAGAACTTGCTGGAAT+TGG 0.422787 tig0028948:+35344 Msa1440090:CDS
AAAGTTTAAAGATGTCAAAA+AGG 0.436388 tig0028948:+34689 Msa1440090:CDS
TACGGTAATTAGGCATCAAA+TGG 0.442752 tig0028948:-33630 None:intergenic
CGCAGAACAATTAGTTCGAA+TGG 0.445789 tig0028948:-33512 None:intergenic
AAGGGTGGCCATGAAGGACT+TGG 0.449428 tig0028948:+33742 Msa1440090:CDS
TAAATTTGTTGCGAGTGATA+AGG 0.460501 tig0028948:+35196 Msa1440090:CDS
AAATGGACATGACGAGGATA+AGG 0.462491 tig0028948:+35169 Msa1440090:CDS
ATACTTGTTCTTTCTATAGG+TGG 0.464692 tig0028948:-33995 None:intergenic
GTAAGAACATGTCATATCTT+GGG 0.464989 tig0028948:+33703 Msa1440090:CDS
GCGTCAATTAGTATGCAATA+AGG 0.467578 tig0028948:+33438 Msa1440090:CDS
AACGCAAAGGAGCTAATTCA+TGG 0.469712 tig0028948:-33603 None:intergenic
AGTGTTAGAATGTGATGATA+GGG 0.470693 tig0028948:-36011 None:intergenic
TTCGAATGGTTAGCGTAGAT+CGG 0.471186 tig0028948:-33498 None:intergenic
AATAGCCGTCATGAGGGAAA+TGG 0.477370 tig0028948:+35152 Msa1440090:CDS
CAAGGGAAAGAGAACTTTGA+TGG 0.478759 tig0028948:+33256 Msa1440090:CDS
CGGACGTGTCCAGAACTTGC+TGG 0.483560 tig0028948:+35338 Msa1440090:CDS
AAAGGACTTATATAATTTCA+CGG 0.493961 tig0028948:+33774 Msa1440090:CDS
GAAATCCTGGGCACACAATA+CGG 0.497533 tig0028948:+35318 Msa1440090:CDS
CACTCTGTAGCAGTGTCAAA+TGG 0.499398 tig0028948:+35566 Msa1440090:CDS
CCAAAAGCGGTGATTACTGA+TGG 0.502215 tig0028948:+34159 Msa1440090:CDS
ATGTGAAGCGTGATTCTAAT+GGG 0.504223 tig0028948:+33404 Msa1440090:CDS
AATGGTGTGACCCGTGACTT+TGG 0.505092 tig0028948:+35845 Msa1440090:CDS
GCAAGGGTTAAAATACAAGA+TGG 0.508614 tig0028948:+33805 Msa1440090:CDS
GTATCACTATGTACATCAAA+AGG 0.509968 tig0028948:-33025 None:intergenic
GGTCAAAAGGGTGGCCATGA+AGG 0.510809 tig0028948:+33736 Msa1440090:CDS
TTTGAGTTACTTCCAAGGTA+AGG 0.511685 tig0028948:+33843 Msa1440090:CDS
CCACGGGTTTGAAGTAAGAA+AGG 0.512169 tig0028948:+33378 Msa1440090:CDS
TTAGAACAACATCTTGTCCT+TGG 0.513299 tig0028948:-35290 None:intergenic
TGTGATGATAGGGTAGTTGT+CGG 0.517812 tig0028948:-36001 None:intergenic
AGTTTGAATCTGAGTGGAAG+AGG 0.520947 tig0028948:+34340 Msa1440090:CDS
ATTTAGTCTGCAACTTGTCA+AGG 0.524883 tig0028948:-34827 None:intergenic
CAAGGGTTAAAATACAAGAT+GGG 0.527779 tig0028948:+33806 Msa1440090:CDS
AACAACGATGTTCGTACTGA+TGG 0.529175 tig0028948:+33184 Msa1440090:CDS
AAAGTCTGATTTGCAAGAGA+TGG 0.531904 tig0028948:+34943 Msa1440090:CDS
GATGTGAAGCGTGATTCTAA+TGG 0.532287 tig0028948:+33403 Msa1440090:CDS
GTTCTAATTGTAGAAATCCT+GGG 0.533951 tig0028948:+35306 Msa1440090:CDS
AAGTGCCCCTCGACGAAACA+TGG 0.534361 tig0028948:-35033 None:intergenic
CACGTCCGTATTGTGTGCCC+AGG 0.534840 tig0028948:-35323 None:intergenic
ACAAATACAAGCACTAGAAA+GGG 0.535750 tig0028948:+35689 Msa1440090:CDS
AGCATTTCGATGCAAATGCC+AGG 0.536176 tig0028948:-34244 None:intergenic
GTAGTTGTCGGAGGGAATTG+TGG 0.539742 tig0028948:-35989 None:intergenic
TTTATCGGTTGAAGAGAATG+TGG 0.541414 tig0028948:+34409 Msa1440090:CDS
GAGTTGTGTTTCCAAATACA+AGG 0.541485 tig0028948:+34208 Msa1440090:CDS
AATAAGGAGGGTGTTAATGA+TGG 0.543414 tig0028948:+33454 Msa1440090:CDS
AAGACAGCCATTGTGCAAGT+TGG 0.545484 tig0028948:+35891 Msa1440090:CDS
AGCTAATTCATGGTTGTGAG+AGG 0.545517 tig0028948:-33593 None:intergenic
TATCAGTATCAGCCTTACCT+TGG 0.546983 tig0028948:-33855 None:intergenic
ACTTCATGGCACAAATAATG+TGG 0.547507 tig0028948:-34896 None:intergenic
CAAAAGCGGTGATTACTGAT+GGG 0.550752 tig0028948:+34160 Msa1440090:CDS
TTGACCTATCAAACAACAGG+TGG 0.553778 tig0028948:+34376 Msa1440090:CDS
GATGCAAATGCCAGGCGCAA+AGG 0.554082 tig0028948:-34236 None:intergenic
TCGTCATTTGCCTCCCAAGA+AGG 0.555407 tig0028948:+34993 Msa1440090:CDS
GTTTGAATCTGAGTGGAAGA+GGG 0.557830 tig0028948:+34341 Msa1440090:CDS
CACTGGTATTACACGTAAGA+GGG 0.559146 tig0028948:+35610 Msa1440090:CDS
GGTGTCGTGTCTGAAGATCA+AGG 0.560464 tig0028948:+35365 Msa1440090:CDS
CGAGATGTTGAGAAAAGCAC+TGG 0.563759 tig0028948:+35593 Msa1440090:CDS
TTCATCATTACCTTCTTGGG+AGG 0.565482 tig0028948:-35003 None:intergenic
GTCTGAAGATCAAGGGGAGA+AGG 0.567087 tig0028948:+35373 Msa1440090:CDS
TATTGTTTGCAAAATACCAC+GGG 0.573778 tig0028948:+33362 Msa1440090:CDS
CCTTTGACATTCACCATCAG+TGG 0.574175 tig0028948:-35242 None:intergenic
GTGGAATTTAACCATGGTAA+TGG 0.576772 tig0028948:+33220 Msa1440090:CDS
GCACAAATAATGTGGGAACA+TGG 0.578248 tig0028948:-34888 None:intergenic
TGTGACAGATGATGATATAA+GGG 0.581249 tig0028948:+33297 Msa1440090:CDS
AAATGACCATGTTTCGTCGA+GGG 0.581274 tig0028948:+35027 Msa1440090:CDS
GTGTCGTGTCTGAAGATCAA+GGG 0.581492 tig0028948:+35366 Msa1440090:CDS
GCTAATTCATGGTTGTGAGA+GGG 0.581523 tig0028948:-33592 None:intergenic
TCAATTAGTATGCAATAAGG+AGG 0.584633 tig0028948:+33441 Msa1440090:CDS
AATGACCATGTTTCGTCGAG+GGG 0.585541 tig0028948:+35028 Msa1440090:CDS
GCAAGTTGGAACCATAATGT+TGG 0.585606 tig0028948:+35905 Msa1440090:CDS
TAAGATAAATAGCCGTCATG+AGG 0.585792 tig0028948:+35145 Msa1440090:CDS
AGGGTGGCCATGAAGGACTT+GGG 0.586883 tig0028948:+33743 Msa1440090:CDS
GAAGCTTCAAAGATATTCAC+CGG 0.587720 tig0028948:+34663 Msa1440090:CDS
AAGATAAATAGCCGTCATGA+GGG 0.587755 tig0028948:+35146 Msa1440090:CDS
ATACTCACCGGTGTATAACT+CGG 0.588936 tig0028948:-36079 None:intergenic
ATGATAGGGTAGTTGTCGGA+GGG 0.591320 tig0028948:-35997 None:intergenic
ATTTCACGGATCATCAGGCA+AGG 0.592596 tig0028948:+33788 Msa1440090:CDS
TGGAACCATAATGTTGGGTA+TGG 0.594448 tig0028948:+35911 Msa1440090:CDS
CTTCATGGCACAAATAATGT+GGG 0.594948 tig0028948:-34895 None:intergenic
TTATCGGTTGAAGAGAATGT+GGG 0.595180 tig0028948:+34410 Msa1440090:CDS
TATGAGTGCGTTGAAGAATG+TGG 0.597255 tig0028948:+36182 Msa1440090:CDS
GAACGAGATGAAGATGAGGA+TGG 0.598765 tig0028948:+34744 Msa1440090:CDS
GTATTGTTTGCAAAATACCA+CGG 0.599210 tig0028948:+33361 Msa1440090:CDS
CAAGTTGGAACCATAATGTT+GGG 0.599464 tig0028948:+35906 Msa1440090:CDS
GTCAATGCAGTTGTCAGCAT+AGG 0.607551 tig0028948:-34627 None:intergenic
CTGAAAAGTTTGAATCTGAG+TGG 0.609008 tig0028948:+34334 Msa1440090:CDS
AAGGGTTAAAATACAAGATG+GGG 0.610552 tig0028948:+33807 Msa1440090:CDS
AAACATCGCAGTGACTATTG+TGG 0.611836 tig0028948:+36216 Msa1440090:CDS
ATTATAAGGAGGCTCTAGAA+GGG 0.613050 tig0028948:+35105 Msa1440090:CDS
GACACTGATAGTGATACAGA+TGG 0.614810 tig0028948:+33082 Msa1440090:CDS
GCTACTTTGAGTTACTTCCA+AGG 0.617165 tig0028948:+33838 Msa1440090:CDS
GTTGATAAACAAGTTGCTAG+TGG 0.617676 tig0028948:+33664 Msa1440090:CDS
TGATAGTGATACAGATGGGG+TGG 0.618742 tig0028948:+33087 Msa1440090:CDS
CATGTCCATTTCCCTCATGA+CGG 0.619225 tig0028948:-35157 None:intergenic
GATGATAGGGTAGTTGTCGG+AGG 0.619255 tig0028948:-35998 None:intergenic
AAATGGTTGTTTCTCCATGG+TGG 0.622494 tig0028948:-34050 None:intergenic
CATTGAACGAGATGAAGATG+AGG 0.623154 tig0028948:+34740 Msa1440090:CDS
ACATAGTGATACAGATGAGG+TGG 0.623452 tig0028948:+33036 Msa1440090:CDS
CCACTGATGGTGAATGTCAA+AGG 0.624002 tig0028948:+35242 Msa1440090:CDS
TATGGTTCCAACTTGCACAA+TGG 0.627946 tig0028948:-35898 None:intergenic
TTTCACGGATCATCAGGCAA+GGG 0.637022 tig0028948:+33789 Msa1440090:CDS
AAAAGCGGTGATTACTGATG+GGG 0.637407 tig0028948:+34161 Msa1440090:CDS
TTTAGTGGATATAACCACCA+TGG 0.638528 tig0028948:+34036 Msa1440090:CDS
AGAACGACATCAATCTGTGA+AGG 0.639278 tig0028948:+34471 Msa1440090:CDS
CCTTTCTTACTTCAAACCCG+TGG 0.639832 tig0028948:-33378 None:intergenic
CATTGCAAACACCATTACCA+TGG 0.642035 tig0028948:-33231 None:intergenic
TATGGGGTGCCAAATAATGG+TGG 0.646881 tig0028948:+35956 Msa1440090:CDS
AATGGTGTTTGCAATGAGCA+AGG 0.648235 tig0028948:+33238 Msa1440090:CDS
GCTAGTGGTCTACATTCGCA+AGG 0.649023 tig0028948:+33679 Msa1440090:CDS
AACAATAGAGACATACAAGT+GGG 0.649289 tig0028948:+34098 Msa1440090:CDS
ATCAAATGGACAAAACGCAA+AGG 0.651947 tig0028948:-33616 None:intergenic
AAAATGACCATGTTTCGTCG+AGG 0.652304 tig0028948:+35026 Msa1440090:CDS
TGACCTATCAAACAACAGGT+GGG 0.653955 tig0028948:+34377 Msa1440090:CDS
ATGGTGTTTGCAATGAGCAA+GGG 0.657603 tig0028948:+33239 Msa1440090:CDS
TCTGTAGCAGTGTCAAATGG+TGG 0.657609 tig0028948:+35569 Msa1440090:CDS
TGATGGAGATGCAGACGGTG+TGG 0.660310 tig0028948:+33201 Msa1440090:CDS
ACACTGATAGTGATACAGAT+GGG 0.663559 tig0028948:+33083 Msa1440090:CDS
TGTCGTGTCTGAAGATCAAG+GGG 0.668190 tig0028948:+35367 Msa1440090:CDS
AAACAATAGAGACATACAAG+TGG 0.671084 tig0028948:+34097 Msa1440090:CDS
TGCCATGAAGTATGACAACA+TGG 0.671427 tig0028948:+34908 Msa1440090:CDS
GTATGACAAACATCCAAAAG+CGG 0.671446 tig0028948:+34146 Msa1440090:CDS
TGAATGTCAAAGGTGCCCGT+AGG 0.674674 tig0028948:+35252 Msa1440090:CDS
CAATTAGTATGCAATAAGGA+GGG 0.675920 tig0028948:+33442 Msa1440090:CDS
TGTAGACGCTTTGAACGTAG+AGG 0.677604 tig0028948:+34861 Msa1440090:CDS
CAATGTTCCGAGTTATACAC+CGG 0.682160 tig0028948:+36072 Msa1440090:CDS
CATTTGACACTGCTACAGAG+TGG 0.682675 tig0028948:-35565 None:intergenic
GAGCGCAAGTGATTATAAGG+AGG 0.683366 tig0028948:+35094 Msa1440090:CDS
TGTTGCGAATGATCCACTGA+TGG 0.687875 tig0028948:+35229 Msa1440090:CDS
GACGGTGTGGAATTTAACCA+TGG 0.688761 tig0028948:+33214 Msa1440090:CDS
TGAGGGAAATGGACATGACG+AGG 0.691164 tig0028948:+35163 Msa1440090:CDS
CGTACTGATGGAGATGCAGA+CGG 0.711480 tig0028948:+33196 Msa1440090:CDS
ACATAGACTATTGAACGCAG+CGG 0.718507 tig0028948:-35057 None:intergenic
GGATATGGAATTCAACAGAG+AGG 0.719788 tig0028948:+33318 Msa1440090:CDS
CACTGATAGTGATACAGATG+GGG 0.724418 tig0028948:+33084 Msa1440090:CDS
TGTACATAGTGATACAGATG+AGG 0.739686 tig0028948:+33033 Msa1440090:CDS
AATTATGCAAGAAATCCACA+AGG 0.743577 tig0028948:-34536 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGAAATTGTATATGATATTT+TGG + tig0028948:35494-35513 Msa1440090:intron 15.0%
!!! AATTATTTTAATATGCACTA+TGG + tig0028948:35938-35957 Msa1440090:CDS 15.0%
!!! ATTATTTTAATATGCACTAT+GGG + tig0028948:35939-35958 Msa1440090:CDS 15.0%
!!! TCATAAACTGTTTTAAATAT+AGG - tig0028948:36167-36186 None:intergenic 15.0%
!! AAAGGACTTATATAATTTCA+CGG + tig0028948:33774-33793 Msa1440090:CDS 20.0%
!! AAAGTTTAAAGATGTCAAAA+AGG + tig0028948:34689-34708 Msa1440090:CDS 20.0%
!!! ATTTTCAAGGTTAATGTATT+TGG + tig0028948:34777-34796 Msa1440090:CDS 20.0%
!!! TTATTTTAATATGCACTATG+GGG + tig0028948:35940-35959 Msa1440090:CDS 20.0%
! GATGGAAAAAATAAGAAGAA+TGG + tig0028948:33472-33491 Msa1440090:CDS 25.0%
! TATCAACTTCACTCAATTTA+CGG - tig0028948:33651-33670 None:intergenic 25.0%
! ATATCCACTAAAAATGACAA+GGG - tig0028948:34028-34047 None:intergenic 25.0%
! TATATCCACTAAAAATGACA+AGG - tig0028948:34029-34048 None:intergenic 25.0%
! AATTAAAGCACAAGCAAAAA+TGG - tig0028948:34070-34089 None:intergenic 25.0%
!!! GGTTCTAAATTTGTTTTCAA+AGG + tig0028948:34119-34138 Msa1440090:CDS 25.0%
! GGTGGGTAAAAAAAATTTAT+CGG + tig0028948:34394-34413 Msa1440090:CDS 25.0%
!! GACATCTTTAAACTTTTTAC+CGG - tig0028948:34685-34704 None:intergenic 25.0%
!! CAAAAAAGATGTAGCTTTTT+CGG + tig0028948:34800-34819 Msa1440090:CDS 25.0%
!! TTTTTCATCATTACCTTCTT+GGG - tig0028948:35009-35028 None:intergenic 25.0%
!!! TTTTTTCATCATTACCTTCT+TGG - tig0028948:35010-35029 None:intergenic 25.0%
!! TCAATGTTGATTGTGATATT+TGG - tig0028948:35731-35750 None:intergenic 25.0%
! CAGCAAATATACTATAACAA+AGG - tig0028948:35764-35783 None:intergenic 25.0%
!!! TTGTTTTGATTGTTGTAGAA+TGG + tig0028948:35827-35846 Msa1440090:intron 25.0%
! TAAACAATCAAAATACTCAC+CGG - tig0028948:36094-36113 None:intergenic 25.0%
GTATCACTATGTACATCAAA+AGG - tig0028948:33028-33047 None:intergenic 30.0%
ATGTGACAGATGATGATATA+AGG + tig0028948:33296-33315 Msa1440090:CDS 30.0%
TGTGACAGATGATGATATAA+GGG + tig0028948:33297-33316 Msa1440090:CDS 30.0%
AGATGATGATATAAGGGATA+TGG + tig0028948:33303-33322 Msa1440090:CDS 30.0%
GTATTGTTTGCAAAATACCA+CGG + tig0028948:33361-33380 Msa1440090:CDS 30.0%
TATTGTTTGCAAAATACCAC+GGG + tig0028948:33362-33381 Msa1440090:CDS 30.0%
TCAATTAGTATGCAATAAGG+AGG + tig0028948:33441-33460 Msa1440090:CDS 30.0%
CAATTAGTATGCAATAAGGA+GGG + tig0028948:33442-33461 Msa1440090:CDS 30.0%
TCACTCAATTTACGGTAATT+AGG - tig0028948:33643-33662 None:intergenic 30.0%
TGTAAGAACATGTCATATCT+TGG + tig0028948:33702-33721 Msa1440090:CDS 30.0%
GTAAGAACATGTCATATCTT+GGG + tig0028948:33703-33722 Msa1440090:CDS 30.0%
!!! TCATATCTTGGGTTTTTTGA+TGG + tig0028948:33714-33733 Msa1440090:CDS 30.0%
!!! CATATCTTGGGTTTTTTGAT+GGG + tig0028948:33715-33734 Msa1440090:CDS 30.0%
ATATAATTTCACGGATCATC+AGG + tig0028948:33783-33802 Msa1440090:CDS 30.0%
CAAGGGTTAAAATACAAGAT+GGG + tig0028948:33806-33825 Msa1440090:CDS 30.0%
AAGGGTTAAAATACAAGATG+GGG + tig0028948:33807-33826 Msa1440090:CDS 30.0%
GAGTTTACATTTACAGATGA+TGG + tig0028948:33892-33911 Msa1440090:CDS 30.0%
!!! ACGCCTAGAAAACATTTTTT+GGG + tig0028948:33915-33934 Msa1440090:CDS 30.0%
ATACTTGTTCTTTCTATAGG+TGG - tig0028948:33998-34017 None:intergenic 30.0%
ACCTATAGAAAGAACAAGTA+TGG + tig0028948:33997-34016 Msa1440090:CDS 30.0%
TCCATACTTGTTCTTTCTAT+AGG - tig0028948:34001-34020 None:intergenic 30.0%
TATCCACTAAAAATGACAAG+GGG - tig0028948:34027-34046 None:intergenic 30.0%
AAACAATAGAGACATACAAG+TGG + tig0028948:34097-34116 Msa1440090:CDS 30.0%
AACAATAGAGACATACAAGT+GGG + tig0028948:34098-34117 Msa1440090:CDS 30.0%
! TTTTGGATGTTTGTCATACA+TGG - tig0028948:34145-34164 None:intergenic 30.0%
!! ATTTTGACCTATCAAACAAC+AGG + tig0028948:34373-34392 Msa1440090:CDS 30.0%
TTCTAACACCACACAAAAAA+TGG - tig0028948:34455-34474 None:intergenic 30.0%
AATTATGCAAGAAATCCACA+AGG - tig0028948:34539-34558 None:intergenic 30.0%
!!! TGGAAACATTGTCATTTTCA+AGG + tig0028948:34764-34783 Msa1440090:CDS 30.0%
!! TAAATTTGTTGCGAGTGATA+AGG + tig0028948:35196-35215 Msa1440090:CDS 30.0%
ATCATTCGCAACAAATTTGT+TGG - tig0028948:35222-35241 None:intergenic 30.0%
TGTTCTAATTGTAGAAATCC+TGG + tig0028948:35305-35324 Msa1440090:CDS 30.0%
GTTCTAATTGTAGAAATCCT+GGG + tig0028948:35306-35325 Msa1440090:CDS 30.0%
GATTCATTATCAGACTCAAT+AGG - tig0028948:35404-35423 None:intergenic 30.0%
AACAAATACAAGCACTAGAA+AGG + tig0028948:35688-35707 Msa1440090:CDS 30.0%
ACAAATACAAGCACTAGAAA+GGG + tig0028948:35689-35708 Msa1440090:CDS 30.0%
! ATTTTTCATGTCCAAAGTCA+CGG - tig0028948:35859-35878 None:intergenic 30.0%
AAAACTGATCCACCATTATT+TGG - tig0028948:35968-35987 None:intergenic 30.0%
AGTGTTAGAATGTGATGATA+GGG - tig0028948:36014-36033 None:intergenic 30.0%
TGTACATAGTGATACAGATG+AGG + tig0028948:33033-33052 Msa1440090:CDS 35.0%
! ACACTGATAGTGATACAGAT+GGG + tig0028948:33083-33102 Msa1440090:CDS 35.0%
GTGGAATTTAACCATGGTAA+TGG + tig0028948:33220-33239 Msa1440090:CDS 35.0%
ATGTGAAGCGTGATTCTAAT+GGG + tig0028948:33404-33423 Msa1440090:CDS 35.0%
GCGTCAATTAGTATGCAATA+AGG + tig0028948:33438-33457 Msa1440090:CDS 35.0%
!! AATAAGGAGGGTGTTAATGA+TGG + tig0028948:33454-33473 Msa1440090:CDS 35.0%
ATCAAATGGACAAAACGCAA+AGG - tig0028948:33619-33638 None:intergenic 35.0%
TACGGTAATTAGGCATCAAA+TGG - tig0028948:33633-33652 None:intergenic 35.0%
! GTTGATAAACAAGTTGCTAG+TGG + tig0028948:33664-33683 Msa1440090:CDS 35.0%
!!! GGTTTTTTGATGGGTCAAAA+GGG + tig0028948:33724-33743 Msa1440090:CDS 35.0%
AGGACTTGGGTTTATCAAAA+AGG + tig0028948:33756-33775 Msa1440090:CDS 35.0%
GCAAGGGTTAAAATACAAGA+TGG + tig0028948:33805-33824 Msa1440090:CDS 35.0%
! TTTGAGTTACTTCCAAGGTA+AGG + tig0028948:33843-33862 Msa1440090:CDS 35.0%
!!! GACGCCTAGAAAACATTTTT+TGG + tig0028948:33914-33933 Msa1440090:CDS 35.0%
! CTGCCCAAAAAATGTTTTCT+AGG - tig0028948:33921-33940 None:intergenic 35.0%
! AGGTTTGACTATGAGTGTTT+TGG + tig0028948:33952-33971 Msa1440090:CDS 35.0%
!!! AAACCCCTTGTCATTTTTAG+TGG + tig0028948:34021-34040 Msa1440090:CDS 35.0%
! TTTAGTGGATATAACCACCA+TGG + tig0028948:34036-34055 Msa1440090:CDS 35.0%
!! CAAAAATGGTTGTTTCTCCA+TGG - tig0028948:34056-34075 None:intergenic 35.0%
GTATGACAAACATCCAAAAG+CGG + tig0028948:34146-34165 Msa1440090:CDS 35.0%
GAGTTGTGTTTCCAAATACA+AGG + tig0028948:34208-34227 Msa1440090:CDS 35.0%
!!! TCAAACTTTTCAGGTGTACA+AGG - tig0028948:34327-34346 None:intergenic 35.0%
! CACTCAGATTCAAACTTTTC+AGG - tig0028948:34336-34355 None:intergenic 35.0%
CTGAAAAGTTTGAATCTGAG+TGG + tig0028948:34334-34353 Msa1440090:CDS 35.0%
! TTTATCGGTTGAAGAGAATG+TGG + tig0028948:34409-34428 Msa1440090:CDS 35.0%
! TTATCGGTTGAAGAGAATGT+GGG + tig0028948:34410-34429 Msa1440090:CDS 35.0%
GAAGCTTCAAAGATATTCAC+CGG + tig0028948:34663-34682 Msa1440090:CDS 35.0%
ATTTAGTCTGCAACTTGTCA+AGG - tig0028948:34830-34849 None:intergenic 35.0%
CTTCATGGCACAAATAATGT+GGG - tig0028948:34898-34917 None:intergenic 35.0%
ACTTCATGGCACAAATAATG+TGG - tig0028948:34899-34918 None:intergenic 35.0%
ATCCATGTTGTCATACTTCA+TGG - tig0028948:34913-34932 None:intergenic 35.0%
! AAAGTCTGATTTGCAAGAGA+TGG + tig0028948:34943-34962 Msa1440090:CDS 35.0%
! TAAGAGCGCAAGTGATTATA+AGG + tig0028948:35091-35110 Msa1440090:CDS 35.0%
!! ATTATAAGGAGGCTCTAGAA+GGG + tig0028948:35105-35124 Msa1440090:CDS 35.0%
TAAGATAAATAGCCGTCATG+AGG + tig0028948:35145-35164 Msa1440090:CDS 35.0%
AAGATAAATAGCCGTCATGA+GGG + tig0028948:35146-35165 Msa1440090:CDS 35.0%
ATCACTCGCAACAAATTTAC+TGG - tig0028948:35195-35214 None:intergenic 35.0%
!!! TGTTGACTTTTTACTCCTAC+GGG - tig0028948:35270-35289 None:intergenic 35.0%
!!! GTGTTGACTTTTTACTCCTA+CGG - tig0028948:35271-35290 None:intergenic 35.0%
TTAGAACAACATCTTGTCCT+TGG - tig0028948:35293-35312 None:intergenic 35.0%
TATCATTACTCATAGACAGC+TGG - tig0028948:35532-35551 None:intergenic 35.0%
!! TGCTGAAAATTGTGTTGCTT+TGG + tig0028948:35779-35798 Msa1440090:intron 35.0%
! TTTTTCATGTCCAAAGTCAC+GGG - tig0028948:35858-35877 None:intergenic 35.0%
! CAAGTTGGAACCATAATGTT+GGG + tig0028948:35906-35925 Msa1440090:CDS 35.0%
TAACACCATACCCAACATTA+TGG - tig0028948:35919-35938 None:intergenic 35.0%
CAGTGTTAGAATGTGATGAT+AGG - tig0028948:36015-36034 None:intergenic 35.0%
ACATAGTGATACAGATGAGG+TGG + tig0028948:33036-33055 Msa1440090:CDS 40.0%
! GACACTGATAGTGATACAGA+TGG + tig0028948:33082-33101 Msa1440090:CDS 40.0%
! CACTGATAGTGATACAGATG+GGG + tig0028948:33084-33103 Msa1440090:CDS 40.0%
AACAACGATGTTCGTACTGA+TGG + tig0028948:33184-33203 Msa1440090:CDS 40.0%
CATTGCAAACACCATTACCA+TGG - tig0028948:33234-33253 None:intergenic 40.0%
AATGGTGTTTGCAATGAGCA+AGG + tig0028948:33238-33257 Msa1440090:CDS 40.0%
ATGGTGTTTGCAATGAGCAA+GGG + tig0028948:33239-33258 Msa1440090:CDS 40.0%
CAAGGGAAAGAGAACTTTGA+TGG + tig0028948:33256-33275 Msa1440090:CDS 40.0%
GGATATGGAATTCAACAGAG+AGG + tig0028948:33318-33337 Msa1440090:CDS 40.0%
GATGTGAAGCGTGATTCTAA+TGG + tig0028948:33403-33422 Msa1440090:CDS 40.0%
TTCGAATGGTTAGCGTAGAT+CGG - tig0028948:33501-33520 None:intergenic 40.0%
CGCAGAACAATTAGTTCGAA+TGG - tig0028948:33515-33534 None:intergenic 40.0%
ACGACTTTGTTCGTACGATT+TGG + tig0028948:33551-33570 Msa1440090:CDS 40.0%
GCTAATTCATGGTTGTGAGA+GGG - tig0028948:33595-33614 None:intergenic 40.0%
AGCTAATTCATGGTTGTGAG+AGG - tig0028948:33596-33615 None:intergenic 40.0%
AACGCAAAGGAGCTAATTCA+TGG - tig0028948:33606-33625 None:intergenic 40.0%
!!! GGGTTTTTTGATGGGTCAAA+AGG + tig0028948:33723-33742 Msa1440090:CDS 40.0%
!! TTTTTGATGGGTCAAAAGGG+TGG + tig0028948:33727-33746 Msa1440090:CDS 40.0%
TGATAAACCCAAGTCCTTCA+TGG - tig0028948:33753-33772 None:intergenic 40.0%
! GCTACTTTGAGTTACTTCCA+AGG + tig0028948:33838-33857 Msa1440090:CDS 40.0%
TATCAGTATCAGCCTTACCT+TGG - tig0028948:33858-33877 None:intergenic 40.0%
TTTGGGCAGATTCTACTAGT+AGG + tig0028948:33932-33951 Msa1440090:CDS 40.0%
!! AAATGGTTGTTTCTCCATGG+TGG - tig0028948:34053-34072 None:intergenic 40.0%
CAAAAGCGGTGATTACTGAT+GGG + tig0028948:34160-34179 Msa1440090:CDS 40.0%
AAAAGCGGTGATTACTGATG+GGG + tig0028948:34161-34180 Msa1440090:CDS 40.0%
AGTTTGAATCTGAGTGGAAG+AGG + tig0028948:34340-34359 Msa1440090:CDS 40.0%
GTTTGAATCTGAGTGGAAGA+GGG + tig0028948:34341-34360 Msa1440090:CDS 40.0%
TTGACCTATCAAACAACAGG+TGG + tig0028948:34376-34395 Msa1440090:CDS 40.0%
TGACCTATCAAACAACAGGT+GGG + tig0028948:34377-34396 Msa1440090:CDS 40.0%
! TTACCCACCTGTTGTTTGAT+AGG - tig0028948:34383-34402 None:intergenic 40.0%
!!! ATGCGAGACCATTTTTTGTG+TGG + tig0028948:34444-34463 Msa1440090:CDS 40.0%
AGAACGACATCAATCTGTGA+AGG + tig0028948:34471-34490 Msa1440090:CDS 40.0%
AGATGTCAAAAAGGCGATAG+AGG + tig0028948:34698-34717 Msa1440090:CDS 40.0%
CATTGAACGAGATGAAGATG+AGG + tig0028948:34740-34759 Msa1440090:CDS 40.0%
GCACAAATAATGTGGGAACA+TGG - tig0028948:34891-34910 None:intergenic 40.0%
TGCCATGAAGTATGACAACA+TGG + tig0028948:34908-34927 Msa1440090:CDS 40.0%
CTCTTGCAAATCAGACTTTC+AGG - tig0028948:34942-34961 None:intergenic 40.0%
TTCATCATTACCTTCTTGGG+AGG - tig0028948:35006-35025 None:intergenic 40.0%
AAAATGACCATGTTTCGTCG+AGG + tig0028948:35026-35045 Msa1440090:CDS 40.0%
AAATGACCATGTTTCGTCGA+GGG + tig0028948:35027-35046 Msa1440090:CDS 40.0%
ACATAGACTATTGAACGCAG+CGG - tig0028948:35060-35079 None:intergenic 40.0%
!! GATTATAAGGAGGCTCTAGA+AGG + tig0028948:35104-35123 Msa1440090:CDS 40.0%
AAATGGACATGACGAGGATA+AGG + tig0028948:35169-35188 Msa1440090:CDS 40.0%
GGAGTAAAAAGTCAACACCA+AGG + tig0028948:35273-35292 Msa1440090:CDS 40.0%
CACTGGTATTACACGTAAGA+GGG + tig0028948:35610-35629 Msa1440090:CDS 40.0%
TATGGTTCCAACTTGCACAA+TGG - tig0028948:35901-35920 None:intergenic 40.0%
! GCAAGTTGGAACCATAATGT+TGG + tig0028948:35905-35924 Msa1440090:CDS 40.0%
TGGAACCATAATGTTGGGTA+TGG + tig0028948:35911-35930 Msa1440090:CDS 40.0%
TGTGATGATAGGGTAGTTGT+CGG - tig0028948:36004-36023 None:intergenic 40.0%
CAATGTTCCGAGTTATACAC+CGG + tig0028948:36072-36091 Msa1440090:CDS 40.0%
ATACTCACCGGTGTATAACT+CGG - tig0028948:36082-36101 None:intergenic 40.0%
TATGAGTGCGTTGAAGAATG+TGG + tig0028948:36182-36201 Msa1440090:CDS 40.0%
AAACATCGCAGTGACTATTG+TGG + tig0028948:36216-36235 Msa1440090:CDS 40.0%
! TGATAGTGATACAGATGGGG+TGG + tig0028948:33087-33106 Msa1440090:CDS 45.0%
GACGGTGTGGAATTTAACCA+TGG + tig0028948:33214-33233 Msa1440090:CDS 45.0%
CCTTTCTTACTTCAAACCCG+TGG - tig0028948:33381-33400 None:intergenic 45.0%
! CCACGGGTTTGAAGTAAGAA+AGG + tig0028948:33378-33397 Msa1440090:CDS 45.0%
ATTTCACGGATCATCAGGCA+AGG + tig0028948:33788-33807 Msa1440090:CDS 45.0%
TTTCACGGATCATCAGGCAA+GGG + tig0028948:33789-33808 Msa1440090:CDS 45.0%
! CCATCAGTAATCACCGCTTT+TGG - tig0028948:34162-34181 None:intergenic 45.0%
CCAAAAGCGGTGATTACTGA+TGG + tig0028948:34159-34178 Msa1440090:CDS 45.0%
CAAAGGCGATGCCTTGTATT+TGG - tig0028948:34222-34241 None:intergenic 45.0%
AGCATTTCGATGCAAATGCC+AGG - tig0028948:34247-34266 None:intergenic 45.0%
!!! CCACAAGGCTGTTTTTGTGT+CGG - tig0028948:34524-34543 None:intergenic 45.0%
GTCAATGCAGTTGTCAGCAT+AGG - tig0028948:34630-34649 None:intergenic 45.0%
GAACGAGATGAAGATGAGGA+TGG + tig0028948:34744-34763 Msa1440090:CDS 45.0%
! TGTAGACGCTTTGAACGTAG+AGG + tig0028948:34861-34880 Msa1440090:CDS 45.0%
AATGACCATGTTTCGTCGAG+GGG + tig0028948:35028-35047 Msa1440090:CDS 45.0%
GAGCGCAAGTGATTATAAGG+AGG + tig0028948:35094-35113 Msa1440090:CDS 45.0%
AATAGCCGTCATGAGGGAAA+TGG + tig0028948:35152-35171 Msa1440090:CDS 45.0%
CATGTCCATTTCCCTCATGA+CGG - tig0028948:35160-35179 None:intergenic 45.0%
TGTTGCGAATGATCCACTGA+TGG + tig0028948:35229-35248 Msa1440090:CDS 45.0%
CCTTTGACATTCACCATCAG+TGG - tig0028948:35245-35264 None:intergenic 45.0%
! CCACTGATGGTGAATGTCAA+AGG + tig0028948:35242-35261 Msa1440090:CDS 45.0%
GAAATCCTGGGCACACAATA+CGG + tig0028948:35318-35337 Msa1440090:CDS 45.0%
!! TGTCCAGAACTTGCTGGAAT+TGG + tig0028948:35344-35363 Msa1440090:CDS 45.0%
ACACCAATTCCAGCAAGTTC+TGG - tig0028948:35350-35369 None:intergenic 45.0%
GTGTCGTGTCTGAAGATCAA+GGG + tig0028948:35366-35385 Msa1440090:CDS 45.0%
TGTCGTGTCTGAAGATCAAG+GGG + tig0028948:35367-35386 Msa1440090:CDS 45.0%
CATTTGACACTGCTACAGAG+TGG - tig0028948:35568-35587 None:intergenic 45.0%
CACTCTGTAGCAGTGTCAAA+TGG + tig0028948:35566-35585 Msa1440090:CDS 45.0%
TCTGTAGCAGTGTCAAATGG+TGG + tig0028948:35569-35588 Msa1440090:CDS 45.0%
! CGAGATGTTGAGAAAAGCAC+TGG + tig0028948:35593-35612 Msa1440090:CDS 45.0%
! GCACTGGTATTACACGTAAG+AGG + tig0028948:35609-35628 Msa1440090:CDS 45.0%
AAGACAGCCATTGTGCAAGT+TGG + tig0028948:35891-35910 Msa1440090:CDS 45.0%
! CACTATGGGGTGCCAAATAA+TGG + tig0028948:35953-35972 Msa1440090:CDS 45.0%
! TATGGGGTGCCAAATAATGG+TGG + tig0028948:35956-35975 Msa1440090:CDS 45.0%
ATGATAGGGTAGTTGTCGGA+GGG - tig0028948:36000-36019 None:intergenic 45.0%
TCTAACACTGCTCTGCATAC+TGG + tig0028948:36025-36044 Msa1440090:CDS 45.0%
ACTGGAACACACAATGTTCC+TGG + tig0028948:36043-36062 Msa1440090:CDS 45.0%
! CGTACTGATGGAGATGCAGA+CGG + tig0028948:33196-33215 Msa1440090:CDS 50.0%
GCTAGTGGTCTACATTCGCA+AGG + tig0028948:33679-33698 Msa1440090:CDS 50.0%
CCGACACAAAAACAGCCTTG+TGG + tig0028948:34521-34540 Msa1440090:CDS 50.0%
TCGTCATTTGCCTCCCAAGA+AGG + tig0028948:34993-35012 Msa1440090:CDS 50.0%
TGAGGGAAATGGACATGACG+AGG + tig0028948:35163-35182 Msa1440090:CDS 50.0%
!! TGAATGTCAAAGGTGCCCGT+AGG + tig0028948:35252-35271 Msa1440090:CDS 50.0%
! GGTGTCGTGTCTGAAGATCA+AGG + tig0028948:35365-35384 Msa1440090:CDS 50.0%
GTCTGAAGATCAAGGGGAGA+AGG + tig0028948:35373-35392 Msa1440090:CDS 50.0%
AATGGTGTGACCCGTGACTT+TGG + tig0028948:35845-35864 Msa1440090:CDS 50.0%
GTAGTTGTCGGAGGGAATTG+TGG - tig0028948:35992-36011 None:intergenic 50.0%
GATGATAGGGTAGTTGTCGG+AGG - tig0028948:36001-36020 None:intergenic 50.0%
CTCGGAACATTGTGTGTTCC+AGG - tig0028948:36064-36083 None:intergenic 50.0%
TGATGGAGATGCAGACGGTG+TGG + tig0028948:33201-33220 Msa1440090:CDS 55.0%
GGTCAAAAGGGTGGCCATGA+AGG + tig0028948:33736-33755 Msa1440090:CDS 55.0%
AAGGGTGGCCATGAAGGACT+TGG + tig0028948:33742-33761 Msa1440090:CDS 55.0%
AGGGTGGCCATGAAGGACTT+GGG + tig0028948:33743-33762 Msa1440090:CDS 55.0%
GATGCAAATGCCAGGCGCAA+AGG - tig0028948:34239-34258 None:intergenic 55.0%
AAGTGCCCCTCGACGAAACA+TGG - tig0028948:35036-35055 None:intergenic 55.0%
CACGTCCGTATTGTGTGCCC+AGG - tig0028948:35326-35345 None:intergenic 60.0%
CGGACGTGTCCAGAACTTGC+TGG + tig0028948:35338-35357 Msa1440090:CDS 60.0%
!! CAAGGCATCGCCTTTGCGCC+TGG + tig0028948:34226-34245 Msa1440090:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0028948 gene 32998 36251 32998 ID=Msa1440090;Name=Msa1440090
tig0028948 mRNA 32998 36251 32998 ID=Msa1440090-mRNA-1;Parent=Msa1440090;Name=Msa1440090-mRNA-1;_AED=0.25;_eAED=0.28;_QI=0|0|0|0.25|1|1|4|0|993
tig0028948 exon 32998 35436 32998 ID=Msa1440090-mRNA-1:exon:12073;Parent=Msa1440090-mRNA-1
tig0028948 exon 35533 35754 35533 ID=Msa1440090-mRNA-1:exon:12074;Parent=Msa1440090-mRNA-1
tig0028948 exon 35845 36093 35845 ID=Msa1440090-mRNA-1:exon:12075;Parent=Msa1440090-mRNA-1
tig0028948 exon 36180 36251 36180 ID=Msa1440090-mRNA-1:exon:12076;Parent=Msa1440090-mRNA-1
tig0028948 CDS 32998 35436 32998 ID=Msa1440090-mRNA-1:cds;Parent=Msa1440090-mRNA-1
tig0028948 CDS 35533 35754 35533 ID=Msa1440090-mRNA-1:cds;Parent=Msa1440090-mRNA-1
tig0028948 CDS 35845 36093 35845 ID=Msa1440090-mRNA-1:cds;Parent=Msa1440090-mRNA-1
tig0028948 CDS 36180 36251 36180 ID=Msa1440090-mRNA-1:cds;Parent=Msa1440090-mRNA-1
Gene Sequence

>Msa1440090

ATGTCTACATATAGTGAAGCAACATTGCCTTTTGATGTACATAGTGATACAGATGAGGTGGAATTTAATAGTGTTAATGATATTGACACTGATAGTGATACAGATGGGGTGGAATTTAATAGTGTTAATGATGTTGACACTGATAGTGACACTGATAGTGATACTGAAGATATAAAAGTCAATAGCAACAACGATGTTCGTACTGATGGAGATGCAGACGGTGTGGAATTTAACCATGGTAATGGTGTTTGCAATGAGCAAGGGAAAGAGAACTTTGATGGCTACAAATGCATCAGTGATGTGACAGATGATGATATAAGGGATATGGAATTCAACAGAGAGGATGAAGCTATAAAGTTTTATGTATTGTTTGCAAAATACCACGGGTTTGAAGTAAGAAAGGATGATGTGAAGCGTGATTCTAATGGGAACATTATTATGCGTCAATTAGTATGCAATAAGGAGGGTGTTAATGATGGAAAAAATAAGAAGAATGGCAGCCGATCTACGCTAACCATTCGAACTAATTGTTCTGCGAAACTTCGAGTAGTCTACGACTTTGTTCGTACGATTTGGAGAGTTTCTATTTTCAAACCCTCTCACAACCATGAATTAGCTCCTTTGCGTTTTGTCCATTTGATGCCTAATTACCGTAAATTGAGTGAAGTTGATAAACAAGTTGCTAGTGGTCTACATTCGCAAGGTGTAAGAACATGTCATATCTTGGGTTTTTTGATGGGTCAAAAGGGTGGCCATGAAGGACTTGGGTTTATCAAAAAGGACTTATATAATTTCACGGATCATCAGGCAAGGGTTAAAATACAAGATGGGGATACTTTTGCTACTTTGAGTTACTTCCAAGGTAAGGCTGATACTGATACATCATTCTTTTCAGAGTTTACATTTACAGATGATGGACGCCTAGAAAACATTTTTTGGGCAGATTCTACTAGTAGGTTTGACTATGAGTGTTTTGGCGATGTTGTTGCATTTGACACCACCTATAGAAAGAACAAGTATGGAAAACCCCTTGTCATTTTTAGTGGATATAACCACCATGGAGAAACAACCATTTTTGCTTGTGCTTTAATTTGTGATGAAACAATAGAGACATACAAGTGGGTTCTAAATTTGTTTTCAAAGGCCATGTATGACAAACATCCAAAAGCGGTGATTACTGATGGGGATAAATCAATGAGAAAAGCTATCAGAGTTGTGTTTCCAAATACAAGGCATCGCCTTTGCGCCTGGCATTTGCATCGAAATGCTGTTCAACATGTGAAGAATTCAAAATTTTTAGAAGAGTTCAAGTCACTGATGTATTCTCCTTGTACACCTGAAAAGTTTGAATCTGAGTGGAAGAGGGTTGTTGAAAATTTTGACCTATCAAACAACAGGTGGGTAAAAAAAATTTATCGGTTGAAGAGAATGTGGGCAAGTGCATACATGCGAGACCATTTTTTGTGTGGTGTTAGAACGACATCAATCTGTGAAGGAGTGAATTCATTTATCAAGAAGTATGTCCGACACAAAAACAGCCTTGTGGATTTCTTGCATAATTTTGAAAGAGCTCTCAAAGAATACAGACATAATGAATTATTGTCTGATTTCAAGTCGTTCTATTATCATCCTATGCTGACAACTGCATTGACTACTTTTGAGTCTGAAGCTTCAAAGATATTCACCGGTAAAAAGTTTAAAGATGTCAAAAAGGCGATAGAGGATGTTGTTGCATTATCAGTCATTGAACGAGATGAAGATGAGGATGGAAACATTGTCATTTTCAAGGTTAATGTATTTGGCAAAAAAGATGTAGCTTTTTCGGTATACCTTGACAAGTTGCAGACTAAATTTTTATGTGATTGTAGACGCTTTGAACGTAGAGGACTGCCATGTTCCCACATTATTTGTGCCATGAAGTATGACAACATGGATGTGTTTCCTGAAAGTCTGATTTGCAAGAGATGGACAAAGACTGCAAAGAGTGATCACATCTCGTCATTTGCCTCCCAAGAAGGTAATGATGAAAAAATGACCATGTTTCGTCGAGGGGCACTTGCCGCTGCGTTCAATAGTCTATGTGATGTTTCGTGTAAGAGCGCAAGTGATTATAAGGAGGCTCTAGAAGGGATTTATAGTCTGTGTAATAAGATAAATAGCCGTCATGAGGGAAATGGACATGACGAGGATAAGGCCAGTAAATTTGTTGCGAGTGATAAGGCCAACAAATTTGTTGCGAATGATCCACTGATGGTGAATGTCAAAGGTGCCCGTAGGAGTAAAAAGTCAACACCAAGGACAAGATGTTGTTCTAATTGTAGAAATCCTGGGCACACAATACGGACGTGTCCAGAACTTGCTGGAATTGGTGTCGTGTCTGAAGATCAAGGGGAGAAGGCTTCACCTATTGAGTCTGATAATGAATCAAAAAGTTTAAGTCTGTCTATGAGTAATGATATGAATGATGACATCCACTCTGTAGCAGTGTCAAATGGTGGACGAGATGTTGAGAAAAGCACTGGTATTACACGTAAGAGGGAATGTGACAATGTTGCATTTTCAACACAAGACTCTGTCAACAAAAGAAAAACAAAAACAAATACAAGCACTAGAAAGGGTGATGCTTGTGTGACTCCAAATATCACAATCAACATTGATAATAATGGTGTGACCCGTGACTTTGGACATGAAAAATCTACTCTCAAACAAGACAGCCATTGTGCAAGTTGGAACCATAATGTTGGGTATGGTGTTAATTATTTTAATATGCACTATGGGGTGCCAAATAATGGTGGATCAGTTTTTCCACAATTCCCTCCGACAACTACCCTATCATCACATTCTAACACTGCTCTGCATACTGGAACACACAATGTTCCTGGAACACACAATGTTCCGAGTTATACACCGTTTATGAGTGCGTTGAAGAATGTGGAGCTGTTAGCAAAACATCGCAGTGACTATTGTGGACCAAAACATTGA
Protein sequence

>Msa1440090

MSTYSEATLPFDVHSDTDEVEFNSVNDIDTDSDTDGVEFNSVNDVDTDSDTDSDTEDIKVNSNNDVRTDGDADGVEFNHGNGVCNEQGKENFDGYKCISDVTDDDIRDMEFNREDEAIKFYVLFAKYHGFEVRKDDVKRDSNGNIIMRQLVCNKEGVNDGKNKKNGSRSTLTIRTNCSAKLRVVYDFVRTIWRVSIFKPSHNHELAPLRFVHLMPNYRKLSEVDKQVASGLHSQGVRTCHILGFLMGQKGGHEGLGFIKKDLYNFTDHQARVKIQDGDTFATLSYFQGKADTDTSFFSEFTFTDDGRLENIFWADSTSRFDYECFGDVVAFDTTYRKNKYGKPLVIFSGYNHHGETTIFACALICDETIETYKWVLNLFSKAMYDKHPKAVITDGDKSMRKAIRVVFPNTRHRLCAWHLHRNAVQHVKNSKFLEEFKSLMYSPCTPEKFESEWKRVVENFDLSNNRWVKKIYRLKRMWASAYMRDHFLCGVRTTSICEGVNSFIKKYVRHKNSLVDFLHNFERALKEYRHNELLSDFKSFYYHPMLTTALTTFESEASKIFTGKKFKDVKKAIEDVVALSVIERDEDEDGNIVIFKVNVFGKKDVAFSVYLDKLQTKFLCDCRRFERRGLPCSHIICAMKYDNMDVFPESLICKRWTKTAKSDHISSFASQEGNDEKMTMFRRGALAAAFNSLCDVSCKSASDYKEALEGIYSLCNKINSRHEGNGHDEDKASKFVASDKANKFVANDPLMVNVKGARRSKKSTPRTRCCSNCRNPGHTIRTCPELAGIGVVSEDQGEKASPIESDNESKSLSLSMSNDMNDDIHSVAVSNGGRDVEKSTGITRKRECDNVAFSTQDSVNKRKTKTNTSTRKGDACVTPNITINIDNNGVTRDFGHEKSTLKQDSHCASWNHNVGYGVNYFNMHYGVPNNGGSVFPQFPPTTTLSSHSNTALHTGTHNVPGTHNVPSYTPFMSALKNVELLAKHRSDYCGPKH*