AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1380040

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1380040 MtrunA17_Chr3g0123821 99.550 222 1 0 1 222 1 222 2.06e-167 459
Msa1380040 MtrunA17_Chr8g0381841 89.732 224 21 2 1 222 1 224 3.77e-151 419
Msa1380040 MtrunA17_Chr1g0174281 73.394 218 56 2 6 221 5 222 1.49e-116 331
Msa1380040 MtrunA17_Chr7g0231131 72.558 215 59 0 6 220 5 219 6.45e-115 327
Msa1380040 MtrunA17_Chr8g0346421 65.438 217 67 2 8 220 18 230 3.92e-97 282
Msa1380040 MtrunA17_Chr7g0275991 66.184 207 67 2 11 216 15 219 4.57e-96 280
Msa1380040 MtrunA17_Chr4g0032201 63.208 212 74 1 10 221 6 213 1.77e-95 277
Msa1380040 MtrunA17_Chr2g0276941 61.321 212 78 2 11 221 10 218 1.59e-92 270
Msa1380040 MtrunA17_Chr3g0112681 62.802 207 75 1 14 220 10 214 7.89e-92 268
Msa1380040 MtrunA17_Chr2g0315551 59.633 218 84 4 4 221 2 215 1.30e-90 265
Msa1380040 MtrunA17_Chr8g0351861 56.561 221 90 3 7 221 6 226 3.47e-90 265
Msa1380040 MtrunA17_Chr2g0319951 64.865 185 64 1 4 188 2 185 2.34e-88 259
Msa1380040 MtrunA17_Chr2g0283131 57.674 215 84 3 9 221 6 215 6.64e-87 256
Msa1380040 MtrunA17_Chr4g0046261 55.814 215 88 2 9 221 6 215 2.69e-86 254
Msa1380040 MtrunA17_Chr2g0325091 55.814 215 91 1 7 221 2 212 6.10e-86 253
Msa1380040 MtrunA17_Chr4g0040781 55.814 215 88 2 9 221 6 215 1.19e-85 253
Msa1380040 MtrunA17_Chr1g0189361 57.820 211 86 1 14 221 12 222 1.33e-85 253
Msa1380040 MtrunA17_Chr7g0214241 55.924 211 84 2 15 221 14 219 9.16e-83 245
Msa1380040 MtrunA17_Chr7g0234201 56.250 208 85 2 15 221 12 214 1.41e-79 237
Msa1380040 MtrunA17_Chr6g0482721 51.887 212 99 1 10 221 6 214 4.28e-75 226
Msa1380040 MtrunA17_Chr8g0392931 53.271 214 94 3 10 221 8 217 3.96e-73 221
Msa1380040 MtrunA17_Chr2g0290891 46.193 197 101 2 15 211 5 196 1.83e-60 188
Msa1380040 MtrunA17_Chr6g0458081 51.515 165 80 0 14 178 4 168 8.85e-60 187
Msa1380040 MtrunA17_Chr8g0335471 44.811 212 105 2 10 221 2 201 2.14e-59 186
Msa1380040 MtrunA17_Chr4g0055651 43.868 212 106 2 10 221 2 200 5.40e-59 184
Msa1380040 MtrunA17_Chr8g0349711 51.829 164 79 0 15 178 5 168 6.06e-59 184
Msa1380040 MtrunA17_Chr7g0250531 43.396 212 107 2 10 221 2 200 1.23e-57 181
Msa1380040 MtrunA17_Chr2g0276931 42.723 213 110 2 9 221 2 202 8.49e-57 179
Msa1380040 MtrunA17_Chr4g0030911 42.584 209 111 3 14 221 13 213 1.82e-56 178
Msa1380040 MtrunA17_Chr8g0349681 50.610 164 81 0 15 178 383 546 1.32e-54 183
Msa1380040 MtrunA17_Chr8g0349681 47.027 185 97 1 10 193 169 353 1.31e-51 175
Msa1380040 MtrunA17_Chr8g0349681 51.923 156 75 0 15 170 12 167 1.47e-50 172
Msa1380040 MtrunA17_Chr1g0212521 42.105 209 113 3 14 221 13 214 1.54e-54 173
Msa1380040 MtrunA17_Chr1g0182321 47.059 170 89 1 9 177 8 177 2.00e-54 173
Msa1380040 MtrunA17_Chr7g0263071 47.879 165 85 1 14 177 13 177 6.12e-54 172
Msa1380040 MtrunA17_Chr1g0182301 46.471 170 90 1 9 177 8 177 6.39e-54 172
Msa1380040 MtrunA17_Chr2g0327911 44.578 166 90 2 14 177 13 178 4.59e-49 159
Msa1380040 MtrunA17_Chr3g0109721 44.509 173 90 1 1 173 1 167 4.66e-49 159
Msa1380040 MtrunA17_Chr1g0199331 47.904 167 83 2 5 170 23 186 8.04e-49 158
Msa1380040 MtrunA17_Chr5g0433561 39.819 221 111 5 1 221 1 199 1.43e-47 155
Msa1380040 MtrunA17_Chr8g0384311 38.140 215 119 5 9 221 6 208 1.23e-46 153
Msa1380040 MtrunA17_Chr8g0390561 37.745 204 110 2 18 221 11 197 2.34e-46 152
Msa1380040 MtrunA17_Chr1g0159481 37.264 212 120 5 12 221 13 213 2.84e-44 147
Msa1380040 MtrunA17_Chr3g0130271 37.383 214 119 6 11 221 7 208 1.02e-42 143
Msa1380040 MtrunA17_Chr8g0382221 34.848 198 113 2 17 198 10 207 1.12e-42 143
Msa1380040 MtrunA17_Chr3g0123971 39.610 154 93 0 17 170 10 163 1.28e-42 143
Msa1380040 MtrunA17_Chr5g0400131 39.610 154 93 0 17 170 10 163 2.43e-42 142
Msa1380040 MtrunA17_Chr7g0265001 45.283 159 85 2 14 170 14 172 2.61e-42 142
Msa1380040 MtrunA17_Chr1g0197331 42.286 175 98 3 9 180 8 182 3.82e-41 139
Msa1380040 MtrunA17_Chr4g0055081 37.662 154 96 0 17 170 10 163 5.25e-41 139
Msa1380040 MtrunA17_Chr1g0180291 42.012 169 95 3 14 180 12 179 4.59e-40 136
Msa1380040 MtrunA17_Chr8g0383261 38.182 165 95 3 16 173 12 176 5.93e-35 123
Msa1380040 MtrunA17_Chr6g0466301 39.394 165 93 3 16 173 8 172 7.97e-35 122
Msa1380040 MtrunA17_Chr6g0465491 39.394 165 93 3 16 173 8 172 7.97e-35 122
Msa1380040 MtrunA17_Chr4g0034871 35.714 196 117 4 16 202 8 203 8.50e-35 122
Msa1380040 MtrunA17_Chr1g0201121 38.788 165 94 3 16 173 8 172 1.40e-34 122
Msa1380040 MtrunA17_Chr2g0331691 37.576 165 96 3 16 173 10 174 6.36e-33 118
Msa1380040 MtrunA17_Chr4g0004661 37.576 165 96 3 16 173 64 228 6.67e-32 117
Msa1380040 MtrunA17_Chr6g0465471 38.514 148 84 4 33 173 7 154 1.85e-26 101
Msa1380040 MtrunA17_Chr6g0466161 38.514 148 84 4 33 173 7 154 1.89e-26 101
Msa1380040 MtrunA17_Chr3g0138111 28.986 207 130 5 11 214 7 199 6.63e-24 95.1
Msa1380040 MtrunA17_Chr3g0138101 28.986 207 130 5 11 214 7 199 7.85e-24 94.7
Msa1380040 MtrunA17_Chr3g0138121 29.126 206 129 5 11 213 7 198 2.92e-23 93.2
Msa1380040 MtrunA17_Chr2g0325101 33.333 165 96 5 18 170 8 170 3.94e-21 87.0
Msa1380040 MtrunA17_Chr4g0046211 35.537 121 75 3 18 137 8 126 5.22e-20 82.8
Msa1380040 MtrunA17_Chr3g0117931 32.121 165 98 5 18 170 10 172 6.30e-20 84.3
Msa1380040 MtrunA17_Chr6g0486041 28.877 187 119 5 18 192 8 192 1.11e-19 83.6
Msa1380040 MtrunA17_Chr4g0037021 35.294 119 74 3 18 135 8 124 2.20e-18 79.7
Msa1380040 MtrunA17_Chr8g0371501 31.325 166 98 6 18 170 8 170 1.09e-17 77.8
Msa1380040 MtrunA17_Chr5g0405791 31.325 166 98 6 18 170 8 170 3.82e-17 76.6
Msa1380040 MtrunA17_Chr6g0466431 37.374 99 53 1 16 114 8 97 5.05e-17 73.6
Msa1380040 MtrunA17_Chr6g0465611 37.374 99 53 1 16 114 8 97 5.05e-17 73.6
Msa1380040 MtrunA17_Chr1g0178291 31.447 159 102 3 19 177 431 582 2.25e-15 74.3
Msa1380040 MtrunA17_Chr5g0443631 26.744 172 114 5 17 180 105 272 1.88e-13 67.8
Msa1380040 MtrunA17_Chr2g0331841 44.828 58 32 0 85 142 2 59 1.09e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1380040 AT5G65270.1 85.135 222 31 2 2 221 3 224 1.21e-138 387
Msa1380040 AT4G39990.1 80.569 211 38 2 12 221 13 221 5.99e-126 355
Msa1380040 AT3G12160.1 75.926 216 51 1 6 221 5 219 8.27e-124 350
Msa1380040 AT5G47960.1 74.528 212 53 1 10 220 9 220 2.59e-115 328
Msa1380040 AT1G09630.1 60.190 211 82 1 10 220 6 214 1.33e-94 276
Msa1380040 AT1G07410.1 61.321 212 77 1 10 221 6 212 5.22e-94 274
Msa1380040 AT4G18800.1 61.009 218 78 3 4 221 2 212 2.74e-92 270
Msa1380040 AT1G16920.1 60.092 218 82 3 4 221 2 214 5.74e-91 266
Msa1380040 AT5G45750.1 60.550 218 81 3 4 221 2 214 4.34e-90 264
Msa1380040 AT3G46830.1 69.143 175 54 0 10 184 6 180 2.08e-89 263
Msa1380040 AT3G46830.2 69.143 175 54 0 10 184 6 180 2.08e-89 263
Msa1380040 AT4G18430.1 56.744 215 86 2 9 221 6 215 2.76e-89 262
Msa1380040 AT5G59150.1 69.540 174 53 0 11 184 7 180 1.62e-88 260
Msa1380040 AT2G33870.1 57.674 215 85 2 9 221 6 216 3.34e-88 259
Msa1380040 AT5G47520.1 57.991 219 90 2 4 221 2 219 4.45e-88 259
Msa1380040 AT1G28550.1 58.605 215 83 2 9 221 6 216 1.33e-87 258
Msa1380040 AT1G06400.1 57.991 219 85 3 4 221 2 214 8.31e-87 256
Msa1380040 AT1G01200.1 60.369 217 75 5 11 220 23 235 9.89e-87 256
Msa1380040 AT5G60860.1 57.674 215 84 3 9 221 6 215 1.29e-86 255
Msa1380040 AT3G15060.1 56.744 215 86 3 9 221 6 215 1.59e-85 253
Msa1380040 AT1G05810.1 63.636 176 64 0 14 189 10 185 2.49e-82 244
Msa1380040 AT1G05810.2 63.636 176 64 0 14 189 56 231 3.67e-82 246
Msa1380040 AT2G43130.1 54.808 208 89 1 14 221 10 212 5.78e-81 241
Msa1380040 AT2G31680.1 61.364 176 68 0 14 189 10 185 2.85e-80 239
Msa1380040 AT3G07410.1 63.068 176 65 0 14 189 10 185 6.55e-79 236
Msa1380040 AT1G73640.1 51.339 224 96 4 11 221 8 231 7.06e-75 226
Msa1380040 AT1G18200.1 50.935 214 98 3 1 214 1 207 2.30e-74 225
Msa1380040 AT5G59150.2 70.400 125 37 0 60 184 5 129 9.38e-62 191
Msa1380040 AT4G17170.1 44.076 211 109 3 15 221 5 210 9.56e-61 189
Msa1380040 AT1G02130.1 46.226 212 102 3 10 221 2 201 1.15e-60 189
Msa1380040 AT4G17160.1 44.340 212 101 3 15 221 5 204 7.47e-60 187
Msa1380040 AT4G35860.1 43.192 213 110 2 14 221 4 210 2.01e-59 186
Msa1380040 AT5G47200.1 44.811 212 104 2 10 221 2 200 1.29e-57 181
Msa1380040 AT4G17530.1 44.811 212 104 2 10 221 2 200 1.33e-57 181
Msa1380040 AT3G11730.1 45.455 209 102 5 14 221 6 203 1.86e-56 178
Msa1380040 AT5G59840.1 41.627 209 114 3 14 221 13 214 2.10e-54 174
Msa1380040 AT3G46060.2 41.627 209 114 3 14 221 13 214 2.37e-54 173
Msa1380040 AT3G46060.3 41.627 209 114 3 14 221 13 214 2.37e-54 173
Msa1380040 AT3G46060.1 41.627 209 114 3 14 221 13 214 2.37e-54 173
Msa1380040 AT5G03520.1 41.121 214 117 3 9 221 8 213 7.33e-53 169
Msa1380040 AT3G53610.2 46.667 165 87 1 14 177 13 177 2.10e-52 168
Msa1380040 AT3G53610.3 46.667 165 87 1 14 177 13 177 2.10e-52 168
Msa1380040 AT3G53610.1 46.667 165 87 1 14 177 13 177 2.10e-52 168
Msa1380040 AT3G54840.2 51.948 154 73 1 18 170 35 188 1.23e-51 166
Msa1380040 AT3G09900.1 41.364 220 110 4 9 221 8 215 1.38e-51 166
Msa1380040 AT3G54840.1 51.948 154 73 1 18 170 35 188 1.75e-51 166
Msa1380040 AT3G54840.3 51.634 153 73 1 19 170 2 154 5.60e-51 163
Msa1380040 AT4G19640.1 37.557 221 116 3 1 221 1 199 6.21e-48 156
Msa1380040 AT1G43890.3 41.232 211 109 6 5 212 2 200 1.18e-46 154
Msa1380040 AT1G43890.1 41.232 211 109 6 5 212 2 200 1.18e-46 154
Msa1380040 AT1G43890.2 41.232 211 109 6 5 212 2 200 1.18e-46 154
Msa1380040 AT5G45130.1 42.775 173 93 1 1 173 1 167 7.15e-46 151
Msa1380040 AT4G35860.2 52.066 121 58 0 58 178 2 122 1.88e-44 146
Msa1380040 AT5G45130.2 44.156 154 86 0 20 173 22 175 6.84e-44 146
Msa1380040 AT2G22290.1 40.260 154 92 0 17 170 10 163 4.70e-43 144
Msa1380040 AT5G10260.1 38.961 154 94 0 17 170 10 163 6.15e-42 141
Msa1380040 AT2G44610.1 38.312 154 95 0 17 170 10 163 2.87e-41 140
Msa1380040 AT4G39890.1 38.065 155 95 1 17 170 10 164 5.17e-41 139
Msa1380040 AT5G03520.2 39.535 172 95 3 51 221 40 203 7.17e-41 139
Msa1380040 AT5G03530.2 36.986 219 125 6 5 221 2 209 1.32e-39 135
Msa1380040 AT5G03530.1 36.986 219 125 6 5 221 2 209 1.32e-39 135
Msa1380040 AT5G64990.1 35.404 161 104 0 17 177 8 168 4.73e-37 129
Msa1380040 AT4G19640.3 37.500 176 103 2 1 176 1 169 5.55e-37 128
Msa1380040 AT4G19640.2 42.958 142 75 2 1 142 1 136 4.15e-36 124
Msa1380040 AT1G22740.1 39.394 165 93 3 16 173 8 172 3.90e-35 124
Msa1380040 AT2G21880.1 39.394 165 93 3 16 173 9 173 4.96e-35 124
Msa1380040 AT3G18820.1 39.394 165 93 3 16 173 8 172 1.73e-34 122
Msa1380040 AT4G09720.1 40.000 165 92 3 16 173 8 172 2.07e-34 122
Msa1380040 AT5G64990.2 33.929 168 104 1 17 177 8 175 2.36e-34 122
Msa1380040 AT1G52280.1 37.647 170 99 3 16 178 8 177 3.58e-34 121
Msa1380040 AT1G49300.2 38.323 167 96 3 16 175 8 174 1.43e-33 120
Msa1380040 AT1G49300.1 38.323 167 96 3 16 175 8 174 1.43e-33 120
Msa1380040 AT4G09720.4 38.824 170 92 3 16 173 8 177 1.48e-33 120
Msa1380040 AT5G46025.1 54.286 105 47 1 78 181 6 110 4.38e-33 115
Msa1380040 AT4G09720.3 37.500 176 92 3 16 173 8 183 1.65e-32 117
Msa1380040 AT3G16100.1 36.970 165 97 3 16 173 8 172 1.18e-31 115
Msa1380040 AT3G09910.3 37.079 178 109 3 5 180 2 178 1.47e-31 114
Msa1380040 AT3G09910.1 37.079 178 109 3 5 180 2 178 1.47e-31 114
Msa1380040 AT3G09910.2 37.079 178 109 3 5 180 2 178 1.47e-31 114
Msa1380040 AT3G09910.5 40.252 159 92 3 14 170 11 168 4.47e-31 113
Msa1380040 AT3G09910.4 40.252 159 92 3 14 170 11 168 4.47e-31 113
Msa1380040 AT5G39620.1 31.604 212 125 5 17 221 6 204 6.44e-30 110
Msa1380040 AT2G21880.4 38.514 148 84 3 33 173 1 148 1.57e-27 103
Msa1380040 AT2G21880.3 38.514 148 84 3 33 173 1 148 1.57e-27 103
Msa1380040 AT5G55080.1 31.548 168 111 3 11 177 7 171 1.16e-25 100
Msa1380040 AT4G09720.6 41.481 135 72 3 46 173 4 138 1.46e-25 98.6
Msa1380040 AT4G09720.5 41.481 135 72 3 46 173 4 138 1.46e-25 98.6
Msa1380040 AT4G09720.2 41.481 135 72 3 46 173 4 138 1.46e-25 98.6
Msa1380040 AT2G21880.2 34.545 165 93 4 16 173 9 165 3.46e-25 98.2
Msa1380040 AT2G44690.1 35.758 165 92 5 18 170 20 182 2.45e-24 96.3
Msa1380040 AT5G20020.1 29.126 206 129 5 11 213 7 198 1.97e-23 94.0
Msa1380040 AT5G55190.1 29.126 206 129 5 11 213 7 198 3.67e-23 93.6
Msa1380040 AT5G20010.1 30.435 184 111 5 11 191 7 176 6.20e-23 92.8
Msa1380040 AT3G48040.1 32.121 165 98 5 18 170 10 172 1.22e-19 84.0
Msa1380040 AT1G20090.1 32.727 165 97 6 18 170 7 169 3.48e-19 82.4
Msa1380040 AT5G45970.1 31.361 169 102 5 18 174 8 174 3.93e-19 82.4
Msa1380040 AT4G35020.1 32.168 143 87 4 18 159 8 141 4.51e-19 82.0
Msa1380040 AT4G35020.2 32.168 143 87 4 18 159 8 141 4.51e-19 82.0
Msa1380040 AT4G35020.3 32.168 143 87 4 18 159 8 141 4.51e-19 82.0
Msa1380040 AT1G75840.1 32.121 165 98 6 18 170 8 170 5.22e-19 82.0
Msa1380040 AT5G62880.1 30.233 172 106 5 18 177 10 179 6.25e-19 82.0
Msa1380040 AT3G48040.2 36.364 121 74 3 18 137 10 128 3.90e-18 79.0
Msa1380040 AT3G51300.1 30.909 165 100 6 18 170 8 170 5.38e-18 79.3
Msa1380040 AT4G28950.1 27.222 180 119 4 17 186 7 184 1.80e-17 78.2
Msa1380040 AT5G62880.2 34.711 121 76 3 18 137 10 128 1.88e-17 77.4
Msa1380040 AT2G17800.2 30.909 165 100 6 18 170 8 170 2.35e-17 77.4
Msa1380040 AT2G17800.1 30.909 165 100 6 18 170 8 170 2.35e-17 77.4
Msa1380040 AT4G35950.1 30.909 165 100 6 18 170 8 170 2.89e-17 77.4
Msa1380040 AT4G08190.1 59.375 64 23 1 107 170 67 127 3.41e-17 75.1
Msa1380040 AT4G35020.4 33.333 114 73 3 18 130 8 119 3.06e-16 73.2
Msa1380040 AT4G35020.5 33.333 114 73 3 18 130 8 119 3.06e-16 73.2
Msa1380040 AT3G22950.1 29.167 120 79 3 17 135 18 132 4.92e-11 60.1
Msa1380040 AT3G22950.2 29.167 120 79 3 17 135 18 132 4.92e-11 60.1

Find 78 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCTCTATATTAGGCTATT+TGG 0.161280 tig0020319:-24547 None:intergenic
TGACAACACATGCTCTTCTT+AGG 0.168776 tig0020319:-24275 None:intergenic
TTAATTAGCGGGTTTGAATT+TGG 0.271621 tig0020319:-21075 None:intergenic
AATATAGTCATCATTCTTAT+AGG 0.279237 tig0020319:+23993 Msa1380040:CDS
CCTGGCTGCTGATGAGAATC+AGG 0.310405 tig0020319:+24187 Msa1380040:CDS
GTAATCAACCTCTCTATATT+AGG 0.319532 tig0020319:-24555 None:intergenic
ATAGGTAGCCTGATTGGAAT+TGG 0.345742 tig0020319:+24495 Msa1380040:three_prime_UTR
ATATTAGGCTATTTAGATAA+GGG 0.350505 tig0020319:-24599 None:intergenic
CATACTACAGGGGCGCTGTT+GGG 0.366375 tig0020319:+23883 Msa1380040:CDS
AGAAAGAAGAGAACCGTTCT+TGG 0.377427 tig0020319:-21381 None:intergenic
GTGATCGAAGCTCGGACGTT+TGG 0.386754 tig0020319:-23928 None:intergenic
ATGTAAATATACACGGGAAA+TGG 0.392454 tig0020319:-24381 None:intergenic
GGCAAGAAGATTATTGTTCC+TGG 0.407195 tig0020319:+24236 Msa1380040:CDS
AATTTCATAGGTAGCCTGAT+TGG 0.411337 tig0020319:+24489 Msa1380040:three_prime_UTR
GACAACACATGCTCTTCTTA+GGG 0.416162 tig0020319:-24274 None:intergenic
TTTGAATTTGGTTTGAAAAG+TGG 0.418073 tig0020319:-21063 None:intergenic
TAGTAACCAGGCTCTATATT+AGG 0.419159 tig0020319:-24614 None:intergenic
AGAGTGTTAAGGCTCAGATC+TGG 0.425788 tig0020319:+21347 Msa1380040:CDS
GGGCGCTGTTGGGGCAATGT+TGG 0.427779 tig0020319:+23893 Msa1380040:CDS
TATATTAGGCTATTTAGATA+AGG 0.432288 tig0020319:-24600 None:intergenic
CCTGATTCTCATCAGCAGCC+AGG 0.437809 tig0020319:-24187 None:intergenic
TTCTTAGGGATTTCTTGTGC+AGG 0.438762 tig0020319:-24260 None:intergenic
TGAGTTTCAAACTCGTACTT+TGG 0.440485 tig0020319:+21312 Msa1380040:CDS
AAAGAATTTGCTGAGAAAGA+AGG 0.441025 tig0020319:+24065 Msa1380040:CDS
GAAGATAGACTACGTCTTCA+AGG 0.447575 tig0020319:+21192 Msa1380040:CDS
GCATACTACAGGGGCGCTGT+TGG 0.449830 tig0020319:+23882 Msa1380040:CDS
TGAAGACGTAGTCTATCTTC+TGG 0.451825 tig0020319:-21189 None:intergenic
ACAAGCGCTACTAATTTCAT+AGG 0.455963 tig0020319:+24477 Msa1380040:three_prime_UTR
CGCAGTTCTTCTAACCATCT+AGG 0.458553 tig0020319:-23954 None:intergenic
AATTGATTAGTTTAATTAGC+GGG 0.466537 tig0020319:-21086 None:intergenic
GTTTAATTTACTAGATTCCA+TGG 0.478421 tig0020319:+24323 Msa1380040:three_prime_UTR
AGGAACAATAATCTTCTTGC+CGG 0.480386 tig0020319:-24234 None:intergenic
ACAATGTTCCAATTCCAATC+AGG 0.483149 tig0020319:-24503 None:intergenic
GCTGATGAGAATCAGGGAAA+TGG 0.488775 tig0020319:+24194 Msa1380040:CDS
TATTGAACATAAGAGTGTTA+AGG 0.489565 tig0020319:+21336 Msa1380040:CDS
CAGTTACAAGTGCATACTAC+AGG 0.490649 tig0020319:+23871 Msa1380040:CDS
AGCATGTGTTGTCAGTAATC+CGG 0.495819 tig0020319:+24284 Msa1380040:exon
GCTTCGATCACATACCTAGA+TGG 0.498381 tig0020319:+23940 Msa1380040:CDS
ATTACCTCCGACTCCATCTC+AGG 0.499272 tig0020319:-24439 None:intergenic
GGCAACTCAGCATCTCTGTC+CGG 0.499309 tig0020319:+24215 Msa1380040:CDS
GGGATTTCTTGTGCAGGACC+AGG 0.502312 tig0020319:-24254 None:intergenic
TGTTGATTTAAGACCTGAGA+TGG 0.509875 tig0020319:+24426 Msa1380040:three_prime_UTR
TTAATCGGTGATTCCGCCGT+TGG 0.519747 tig0020319:+21220 Msa1380040:CDS
ATACTACAGGGGCGCTGTTG+GGG 0.525539 tig0020319:+23884 Msa1380040:CDS
AGAGAGGTTGATTACTGCTA+TGG 0.526309 tig0020319:+24563 Msa1380040:three_prime_UTR
GCTCAGATCTGGGACACTGC+TGG 0.529753 tig0020319:+21358 Msa1380040:CDS
GAGTGTTAAGGCTCAGATCT+GGG 0.538288 tig0020319:+21348 Msa1380040:CDS
GCAAGAACAATGGCGAGTGC+AGG 0.539693 tig0020319:+21151 Msa1380040:exon
TTCTGTCAAAACAGTCATGA+AGG 0.542515 tig0020319:-24138 None:intergenic
ACAATGGCGAGTGCAGGTTA+CGG 0.545612 tig0020319:+21157 Msa1380040:exon
ATGGCCAAACCATCAAAACT+TGG 0.558491 tig0020319:-24362 None:intergenic
AAATAGCCTAATATAGAGCC+TGG 0.566946 tig0020319:+24608 Msa1380040:three_prime_UTR
GTGGACTCAAAAGCTACAAT+CGG 0.568023 tig0020319:+21286 Msa1380040:CDS
CATTGTCAACAAGAAGAACC+TGG 0.569186 tig0020319:+24169 Msa1380040:CDS
TATTAGGCTATTTAGATAAG+GGG 0.572036 tig0020319:-24598 None:intergenic
GGGACACTGCTGGCCAAGAA+CGG 0.580774 tig0020319:+21368 Msa1380040:CDS
AGGAAACAAAAGTGATCTTG+AGG 0.580983 tig0020319:+24013 Msa1380040:CDS
CTAGGTATGTGATCGAAGCT+CGG 0.584273 tig0020319:-23936 None:intergenic
GAAAATCTCAGATACTAGCT+CGG 0.589018 tig0020319:+21242 Msa1380040:CDS
TTGAATGATGTAAATATACA+CGG 0.596315 tig0020319:-24388 None:intergenic
TGAATGATGTAAATATACAC+GGG 0.603917 tig0020319:-24387 None:intergenic
AGTTACAAGTGCATACTACA+GGG 0.605565 tig0020319:+23872 Msa1380040:CDS
TAATTGATTAGTTTAATTAG+CGG 0.611155 tig0020319:-21087 None:intergenic
TTTAAGACCTGAGATGGAGT+CGG 0.620598 tig0020319:+24432 Msa1380040:three_prime_UTR
CTGGCTGCTGATGAGAATCA+GGG 0.625486 tig0020319:+24188 Msa1380040:CDS
CCAAATAGCCTAATATAGAG+AGG 0.634926 tig0020319:+24547 Msa1380040:three_prime_UTR
TGACTATATTCTTGTCAGCA+TGG 0.639191 tig0020319:-23980 None:intergenic
GAAATCAAAAGCAAGAACAA+TGG 0.640264 tig0020319:+21141 Msa1380040:exon
ATTAGTTGCTTGCAGCGCAG+AGG 0.640396 tig0020319:-24105 None:intergenic
GCAAGCAACTAATGTTGAGA+CGG 0.646912 tig0020319:+24115 Msa1380040:CDS
GGTTGATTACTGCTATGGTG+TGG 0.657425 tig0020319:+24568 Msa1380040:three_prime_UTR
GAAGTAATTCAGTAGTAACC+AGG 0.665431 tig0020319:-24626 None:intergenic
AAGACCTGAGATGGAGTCGG+AGG 0.667296 tig0020319:+24435 Msa1380040:three_prime_UTR
GTCATACGAACATAGAACCA+TGG 0.670149 tig0020319:-24340 None:intergenic
TGCAAGAAACGACTTCAGCG+TGG 0.696247 tig0020319:+21267 Msa1380040:CDS
GTTACAAGTGCATACTACAG+GGG 0.696627 tig0020319:+23873 Msa1380040:CDS
GCAGCGTGCAGTACCCACAG+AGG 0.703658 tig0020319:+24037 Msa1380040:CDS
GATAGACTACGTCTTCAAGG+TGG 0.714276 tig0020319:+21195 Msa1380040:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATTTTTTTTTTTAAAAACT+CGG - tig0020319:22007-22026 None:intergenic 10.0%
!!! AATTATATCAAATTTAAAAC+TGG - tig0020319:22422-22441 None:intergenic 10.0%
!!! TAATTGATTAGTTTAATTAG+CGG - tig0020319:21090-21109 None:intergenic 15.0%
!! TATTCCTCAATAAAAAAAAT+TGG - tig0020319:21643-21662 None:intergenic 15.0%
!!! GAAGAAAAACATAAATTTTA+TGG + tig0020319:22670-22689 Msa1380040:intron 15.0%
!!! ACTGTTGATAAATAAATATT+TGG - tig0020319:23034-23053 None:intergenic 15.0%
!! GTAATTTAAATATTGGTATT+TGG - tig0020319:23783-23802 None:intergenic 15.0%
!! TGTACTTGTAATTTAAATAT+TGG - tig0020319:23790-23809 None:intergenic 15.0%
!!! AATTGATTAGTTTAATTAGC+GGG - tig0020319:21089-21108 None:intergenic 20.0%
!!! GTTTATTTATCTGAAGTTAA+TGG + tig0020319:21529-21548 Msa1380040:intron 20.0%
!!! AACACCAATTTTTTTTATTG+AGG + tig0020319:21636-21655 Msa1380040:intron 20.0%
!! AAAAAAATCTAGTATGTGAA+TGG + tig0020319:22018-22037 Msa1380040:intron 20.0%
!!! ATGATTTTTATTTCAAAGAG+TGG - tig0020319:23522-23541 None:intergenic 20.0%
!!! ATTTTCAAAATCAGCAATTT+TGG + tig0020319:23594-23613 Msa1380040:intron 20.0%
!! TTGATTGAGTAAAAAAACAT+GGG - tig0020319:23669-23688 None:intergenic 20.0%
!! ATTGATTGAGTAAAAAAACA+TGG - tig0020319:23670-23689 None:intergenic 20.0%
!!! GTTTTGTCTGATTTTATAAA+TGG + tig0020319:23826-23845 Msa1380040:intron 20.0%
!! AATATAGTCATCATTCTTAT+AGG + tig0020319:23993-24012 Msa1380040:CDS 20.0%
!!! AACATTTTATAAATGAACTC+CGG - tig0020319:24306-24325 None:intergenic 20.0%
!! TTGAATGATGTAAATATACA+CGG - tig0020319:24391-24410 None:intergenic 20.0%
!! ATATTAGGCTATTTAGATAA+GGG - tig0020319:24602-24621 None:intergenic 20.0%
!! TATATTAGGCTATTTAGATA+AGG - tig0020319:24603-24622 None:intergenic 20.0%
!!! TTTGAATTTGGTTTGAAAAG+TGG - tig0020319:21066-21085 None:intergenic 25.0%
!!! TATTGAACATAAGAGTGTTA+AGG + tig0020319:21336-21355 Msa1380040:CDS 25.0%
!!! TTGTTTATTGTTTTTGCCTA+TGG + tig0020319:21835-21854 Msa1380040:intron 25.0%
! TATGTGAATTAACCCATTTA+GGG + tig0020319:21956-21975 Msa1380040:intron 25.0%
! AGTAGAAATATTACAGTTTG+CGG + tig0020319:22117-22136 Msa1380040:intron 25.0%
!! TGATTTTACCATAAGTTCAA+AGG - tig0020319:22254-22273 None:intergenic 25.0%
!!! ACCATATTTTGATTTGAAAC+CGG - tig0020319:22278-22297 None:intergenic 25.0%
!!! ATGTATTGACTGATTTCAAT+TGG + tig0020319:22353-22372 Msa1380040:intron 25.0%
!! TCAAATTTAAAACTGGAATG+TGG - tig0020319:22415-22434 None:intergenic 25.0%
! TGATTATTTATAGAGCAGTT+AGG + tig0020319:22604-22623 Msa1380040:intron 25.0%
! GAACCAAATCATTTATTCAT+GGG - tig0020319:22917-22936 None:intergenic 25.0%
!! ACCTTTTGTACATATTAACA+GGG - tig0020319:23456-23475 None:intergenic 25.0%
!! AACCTTTTGTACATATTAAC+AGG - tig0020319:23457-23476 None:intergenic 25.0%
!! TATCAATAACACGTTTTCAA+AGG - tig0020319:23571-23590 None:intergenic 25.0%
!!! GTTTTGCACTATTTTTTCTA+CGG + tig0020319:23707-23726 Msa1380040:intron 25.0%
! TAAATTACAAGTACATCGAA+TGG + tig0020319:23795-23814 Msa1380040:intron 25.0%
!! GTTTAATTTACTAGATTCCA+TGG + tig0020319:24323-24342 Msa1380040:three_prime_UTR 25.0%
! TGAATGATGTAAATATACAC+GGG - tig0020319:24390-24409 None:intergenic 25.0%
! TATTAGGCTATTTAGATAAG+GGG - tig0020319:24601-24620 None:intergenic 25.0%
!! TTAATTAGCGGGTTTGAATT+TGG - tig0020319:21078-21097 None:intergenic 30.0%
!! TTGGTTTTCGATTTTGAATG+TGG - tig0020319:21121-21140 None:intergenic 30.0%
!!! TGTTCTTGCTTTTGATTTCT+TGG - tig0020319:21140-21159 None:intergenic 30.0%
GAAATCAAAAGCAAGAACAA+TGG + tig0020319:21141-21160 Msa1380040:exon 30.0%
!! TAGTATCTGAGATTTTCCAA+CGG - tig0020319:21239-21258 None:intergenic 30.0%
ACATCAGTCAAACTAATTCA+AGG + tig0020319:21687-21706 Msa1380040:intron 30.0%
GTGTCAAACAAATTCAAGAT+TGG - tig0020319:21739-21758 None:intergenic 30.0%
! TTTTTGCCTATGGTTTCTTT+GGG + tig0020319:21845-21864 Msa1380040:intron 30.0%
GTATGTGAATTAACCCATTT+AGG + tig0020319:21955-21974 Msa1380040:intron 30.0%
CAGATTTATGAAACTTACGA+GGG + tig0020319:22058-22077 Msa1380040:intron 30.0%
AGATTTATGAAACTTACGAG+GGG + tig0020319:22059-22078 Msa1380040:intron 30.0%
TCCATATAGTATGCTAATAG+TGG + tig0020319:22189-22208 Msa1380040:intron 30.0%
ACCACTATTAGCATACTATA+TGG - tig0020319:22193-22212 None:intergenic 30.0%
CAATGATTCCTTTGAACTTA+TGG + tig0020319:22243-22262 Msa1380040:intron 30.0%
GAACTTATGGTAAAATCATC+CGG + tig0020319:22256-22275 Msa1380040:intron 30.0%
TCCGGTTTCAAATCAAAATA+TGG + tig0020319:22274-22293 Msa1380040:intron 30.0%
GTTATATAAGTCCACAGATT+TGG - tig0020319:22379-22398 None:intergenic 30.0%
TGTTCATATACATAGTGTGT+TGG - tig0020319:22463-22482 None:intergenic 30.0%
ATGTAGTTGTAAAGAGAAGA+AGG + tig0020319:22735-22754 Msa1380040:intron 30.0%
! TATGTTGCTTAAACAAAGCT+GGG + tig0020319:22769-22788 Msa1380040:intron 30.0%
GGAACCAAATCATTTATTCA+TGG - tig0020319:22918-22937 None:intergenic 30.0%
! ACTTTACTGATTACTCACTT+TGG - tig0020319:22942-22961 None:intergenic 30.0%
CAGTAAAGTGAGAAAATCAA+TGG + tig0020319:22953-22972 Msa1380040:intron 30.0%
! AGCAAGTATCTGTTACTTAA+GGG - tig0020319:23276-23295 None:intergenic 30.0%
AAATACTTGTAGGGAGAAAT+GGG - tig0020319:23317-23336 None:intergenic 30.0%
ACCCTGTTAATATGTACAAA+AGG + tig0020319:23452-23471 Msa1380040:intron 30.0%
!! GCTGATTATTTTTCTGTCTT+GGG + tig0020319:23616-23635 Msa1380040:intron 30.0%
TCAATCAATGTTCTTCAATC+TGG + tig0020319:23681-23700 Msa1380040:intron 30.0%
TACTTCTGATCATTTCATCA+TGG + tig0020319:23755-23774 Msa1380040:intron 30.0%
!! AAAGAATTTGCTGAGAAAGA+AGG + tig0020319:24065-24084 Msa1380040:CDS 30.0%
ATGTAAATATACACGGGAAA+TGG - tig0020319:24384-24403 None:intergenic 30.0%
GTAATCAACCTCTCTATATT+AGG - tig0020319:24558-24577 None:intergenic 30.0%
! TCGATTTTGAATGTGGAGTT+GGG - tig0020319:21114-21133 None:intergenic 35.0%
! TTCGATTTTGAATGTGGAGT+TGG - tig0020319:21115-21134 None:intergenic 35.0%
!!! GTCTTCAAGGTGGTTTTAAT+CGG + tig0020319:21205-21224 Msa1380040:CDS 35.0%
GAAAATCTCAGATACTAGCT+CGG + tig0020319:21242-21261 Msa1380040:CDS 35.0%
TGAGTTTCAAACTCGTACTT+TGG + tig0020319:21312-21331 Msa1380040:CDS 35.0%
! GTTTTTGCCTATGGTTTCTT+TGG + tig0020319:21844-21863 Msa1380040:intron 35.0%
CGCAGGATTTAAGTAAGAAA+AGG + tig0020319:21903-21922 Msa1380040:intron 35.0%
! TGAATTAACCCATTTAGGGT+TGG + tig0020319:21960-21979 Msa1380040:intron 35.0%
GCAGATTTATGAAACTTACG+AGG + tig0020319:22057-22076 Msa1380040:intron 35.0%
GTTCAAAGGAATCATTGCTA+AGG - tig0020319:22240-22259 None:intergenic 35.0%
ATTTCAATTGGCCAAATCTG+TGG + tig0020319:22365-22384 Msa1380040:intron 35.0%
!! TGTTGGTAGCATTTCTAACA+TGG - tig0020319:22446-22465 None:intergenic 35.0%
ATTTACTTGAATGACACGTG+TGG + tig0020319:22551-22570 Msa1380040:intron 35.0%
! ACATTGATGGGAAATGGATT+TGG + tig0020319:22630-22649 Msa1380040:intron 35.0%
ATTTGGAAGTTGGGAGAAAA+TGG + tig0020319:22647-22666 Msa1380040:intron 35.0%
TTTGGAAGTTGGGAGAAAAT+GGG + tig0020319:22648-22667 Msa1380040:intron 35.0%
! GTATGTTGCTTAAACAAAGC+TGG + tig0020319:22768-22787 Msa1380040:intron 35.0%
TAGGAGTTACTGAGATTTCA+GGG + tig0020319:22857-22876 Msa1380040:intron 35.0%
CAGCCCATGAATAAATGATT+TGG + tig0020319:22911-22930 Msa1380040:intron 35.0%
! TTACTGATTACTCACTTTGG+AGG - tig0020319:22939-22958 None:intergenic 35.0%
GCACATGCATGATAAATCAT+GGG - tig0020319:22994-23013 None:intergenic 35.0%
TGCACATGCATGATAAATCA+TGG - tig0020319:22995-23014 None:intergenic 35.0%
CTCTAATTTATTGCTGGATC+AGG - tig0020319:23106-23125 None:intergenic 35.0%
GCAAGACTCTAATTTATTGC+TGG - tig0020319:23112-23131 None:intergenic 35.0%
TTACTTAAGGGAGTTAGAGT+TGG - tig0020319:23264-23283 None:intergenic 35.0%
! GAGCAAGTATCTGTTACTTA+AGG - tig0020319:23277-23296 None:intergenic 35.0%
GAAATACTTGTAGGGAGAAA+TGG - tig0020319:23318-23337 None:intergenic 35.0%
CCAGAAAGGAAATACTTGTA+GGG - tig0020319:23326-23345 None:intergenic 35.0%
ATTCTGTGATACTAGCATAC+TGG - tig0020319:23412-23431 None:intergenic 35.0%
!! GGCTGATTATTTTTCTGTCT+TGG + tig0020319:23615-23634 Msa1380040:intron 35.0%
AGTTACAAGTGCATACTACA+GGG + tig0020319:23872-23891 Msa1380040:CDS 35.0%
TGACTATATTCTTGTCAGCA+TGG - tig0020319:23983-24002 None:intergenic 35.0%
AGGAAACAAAAGTGATCTTG+AGG + tig0020319:24013-24032 Msa1380040:CDS 35.0%
! AATTCTTTTGCATCCTCTGT+GGG - tig0020319:24053-24072 None:intergenic 35.0%
! AAATTCTTTTGCATCCTCTG+TGG - tig0020319:24054-24073 None:intergenic 35.0%
TTCTGTCAAAACAGTCATGA+AGG - tig0020319:24141-24160 None:intergenic 35.0%
AGGAACAATAATCTTCTTGC+CGG - tig0020319:24237-24256 None:intergenic 35.0%
!! TTCGTATGACCAAGTTTTGA+TGG + tig0020319:24353-24372 Msa1380040:three_prime_UTR 35.0%
!! ATGACCAAGTTTTGATGGTT+TGG + tig0020319:24358-24377 Msa1380040:three_prime_UTR 35.0%
!! TGTTGATTTAAGACCTGAGA+TGG + tig0020319:24426-24445 Msa1380040:three_prime_UTR 35.0%
ACAAGCGCTACTAATTTCAT+AGG + tig0020319:24477-24496 Msa1380040:three_prime_UTR 35.0%
AATTTCATAGGTAGCCTGAT+TGG + tig0020319:24489-24508 Msa1380040:three_prime_UTR 35.0%
ACAATGTTCCAATTCCAATC+AGG - tig0020319:24506-24525 None:intergenic 35.0%
CCTCTCTATATTAGGCTATT+TGG - tig0020319:24550-24569 None:intergenic 35.0%
CCAAATAGCCTAATATAGAG+AGG + tig0020319:24547-24566 Msa1380040:three_prime_UTR 35.0%
AAATAGCCTAATATAGAGCC+TGG + tig0020319:24608-24627 Msa1380040:three_prime_UTR 35.0%
TAGTAACCAGGCTCTATATT+AGG - tig0020319:24617-24636 None:intergenic 35.0%
GAAGTAATTCAGTAGTAACC+AGG - tig0020319:24629-24648 None:intergenic 35.0%
TGAAGACGTAGTCTATCTTC+TGG - tig0020319:21192-21211 None:intergenic 40.0%
GAAGATAGACTACGTCTTCA+AGG + tig0020319:21192-21211 Msa1380040:CDS 40.0%
!! TATCTGAGATTTTCCAACGG+CGG - tig0020319:21236-21255 None:intergenic 40.0%
GTGGACTCAAAAGCTACAAT+CGG + tig0020319:21286-21305 Msa1380040:CDS 40.0%
AGAAAGAAGAGAACCGTTCT+TGG - tig0020319:21384-21403 None:intergenic 40.0%
ACTCATCCCAAAGAAACCAT+AGG - tig0020319:21854-21873 None:intergenic 40.0%
GTATGCATTCGACATGAATG+AGG - tig0020319:22311-22330 None:intergenic 40.0%
GTTGGATTTCATACGTCACA+AGG + tig0020319:22573-22592 Msa1380040:intron 40.0%
AGCAGTTAGGAAGACATTGA+TGG + tig0020319:22617-22636 Msa1380040:intron 40.0%
GCAGTTAGGAAGACATTGAT+GGG + tig0020319:22618-22637 Msa1380040:intron 40.0%
AGGAAGACATTGATGGGAAA+TGG + tig0020319:22624-22643 Msa1380040:intron 40.0%
! TGGGAAATGGATTTGGAAGT+TGG + tig0020319:22637-22656 Msa1380040:intron 40.0%
! GGGAAATGGATTTGGAAGTT+GGG + tig0020319:22638-22657 Msa1380040:intron 40.0%
GGGTCAGAAAGTAAGAAGAA+CGG + tig0020319:22789-22808 Msa1380040:intron 40.0%
GGTCAGAAAGTAAGAAGAAC+GGG + tig0020319:22790-22809 Msa1380040:intron 40.0%
CTAGGAGTTACTGAGATTTC+AGG + tig0020319:22856-22875 Msa1380040:intron 40.0%
CATGCATGATAAATCATGGG+TGG - tig0020319:22991-23010 None:intergenic 40.0%
GTAGTTGTGTTTAGAGGGAA+AGG - tig0020319:23134-23153 None:intergenic 40.0%
TAGCGGTAGTTGTGTTTAGA+GGG - tig0020319:23139-23158 None:intergenic 40.0%
TTAGCGGTAGTTGTGTTTAG+AGG - tig0020319:23140-23159 None:intergenic 40.0%
CAATAGGGGATTCAGTTTAG+CGG - tig0020319:23156-23175 None:intergenic 40.0%
ATGTGTGGAGAGAACAATAG+GGG - tig0020319:23170-23189 None:intergenic 40.0%
GATGTGTGGAGAGAACAATA+GGG - tig0020319:23171-23190 None:intergenic 40.0%
AGGTAGGTTATAAGGATGTG+TGG - tig0020319:23185-23204 None:intergenic 40.0%
ATTGGCAGAGGTAGGTTATA+AGG - tig0020319:23193-23212 None:intergenic 40.0%
CCCTACAAGTATTTCCTTTC+TGG + tig0020319:23323-23342 Msa1380040:intron 40.0%
GCCAGAAAGGAAATACTTGT+AGG - tig0020319:23327-23346 None:intergenic 40.0%
CAGTTACAAGTGCATACTAC+AGG + tig0020319:23871-23890 Msa1380040:CDS 40.0%
GTTACAAGTGCATACTACAG+GGG + tig0020319:23873-23892 Msa1380040:CDS 40.0%
GCAAGCAACTAATGTTGAGA+CGG + tig0020319:24115-24134 Msa1380040:CDS 40.0%
CATTGTCAACAAGAAGAACC+TGG + tig0020319:24169-24188 Msa1380040:CDS 40.0%
GGCAAGAAGATTATTGTTCC+TGG + tig0020319:24236-24255 Msa1380040:CDS 40.0%
TTCTTAGGGATTTCTTGTGC+AGG - tig0020319:24263-24282 None:intergenic 40.0%
GACAACACATGCTCTTCTTA+GGG - tig0020319:24277-24296 None:intergenic 40.0%
TGACAACACATGCTCTTCTT+AGG - tig0020319:24278-24297 None:intergenic 40.0%
AGCATGTGTTGTCAGTAATC+CGG + tig0020319:24284-24303 Msa1380040:exon 40.0%
GTCATACGAACATAGAACCA+TGG - tig0020319:24343-24362 None:intergenic 40.0%
ATGGCCAAACCATCAAAACT+TGG - tig0020319:24365-24384 None:intergenic 40.0%
! TTTAAGACCTGAGATGGAGT+CGG + tig0020319:24432-24451 Msa1380040:three_prime_UTR 40.0%
ATAGGTAGCCTGATTGGAAT+TGG + tig0020319:24495-24514 Msa1380040:three_prime_UTR 40.0%
! AGAGAGGTTGATTACTGCTA+TGG + tig0020319:24563-24582 Msa1380040:three_prime_UTR 40.0%
! GATTTTTACCACGAAGTGTG+TGG + tig0020319:24651-24670 Msa1380040:three_prime_UTR 40.0%
! AATGTTTTCCACACACTTCG+TGG - tig0020319:24662-24681 None:intergenic 40.0%
GATAGACTACGTCTTCAAGG+TGG + tig0020319:21195-21214 Msa1380040:CDS 45.0%
! AGAGTGTTAAGGCTCAGATC+TGG + tig0020319:21347-21366 Msa1380040:CDS 45.0%
GAGTGTTAAGGCTCAGATCT+GGG + tig0020319:21348-21367 Msa1380040:CDS 45.0%
!! GTTTGACTGATGTTGACTCG+AGG - tig0020319:21771-21790 None:intergenic 45.0%
!! GTTTGACTGATGTTGACTCG+AGG + tig0020319:21771-21790 Msa1380040:intron 45.0%
AGTTTGAGTGTGTATGAGCG+AGG + tig0020319:21871-21890 Msa1380040:intron 45.0%
!! TAACCCATTTAGGGTTGGCT+TGG + tig0020319:21965-21984 Msa1380040:intron 45.0%
!! AACCCATTTAGGGTTGGCTT+GGG + tig0020319:21966-21985 Msa1380040:intron 45.0%
AACCCAAGCCAACCCTAAAT+GGG - tig0020319:21971-21990 None:intergenic 45.0%
GCGGCATATTTGAACTTCAG+TGG + tig0020319:22136-22155 Msa1380040:intron 45.0%
CATACTGCCTGACTTAACTC+AGG + tig0020319:22326-22345 Msa1380040:intron 45.0%
CATGTCACCTGAGTTAAGTC+AGG - tig0020319:22336-22355 None:intergenic 45.0%
ACTTGAATGACACGTGTGGT+TGG + tig0020319:22555-22574 Msa1380040:intron 45.0%
CTCAGTAACTCCTAGCTGTT+GGG - tig0020319:22851-22870 None:intergenic 45.0%
TCTCAGTAACTCCTAGCTGT+TGG - tig0020319:22852-22871 None:intergenic 45.0%
TGTGAGCAACTCACTGTAGA+GGG - tig0020319:23074-23093 None:intergenic 45.0%
TTGTGAGCAACTCACTGTAG+AGG - tig0020319:23075-23094 None:intergenic 45.0%
CAGTGAGTTGCTCACAATAG+AGG + tig0020319:23078-23097 Msa1380040:intron 45.0%
GGATGTGTGGAGAGAACAAT+AGG - tig0020319:23172-23191 None:intergenic 45.0%
AGATAGCACTCTCACAGGAT+TGG - tig0020319:23211-23230 None:intergenic 45.0%
!! AATCCTGTGAGAGTGCTATC+TGG + tig0020319:23210-23229 Msa1380040:intron 45.0%
GTAACAGATACTTGCTCAGC+TGG + tig0020319:23280-23299 Msa1380040:intron 45.0%
GCTGTGTGCTTACAGACATT+GGG + tig0020319:23345-23364 Msa1380040:intron 45.0%
GGTGCATGAGTTGTACACTA+AGG - tig0020319:23435-23454 None:intergenic 45.0%
CTAGGTATGTGATCGAAGCT+CGG - tig0020319:23939-23958 None:intergenic 45.0%
GCTTCGATCACATACCTAGA+TGG + tig0020319:23940-23959 Msa1380040:CDS 45.0%
CGCAGTTCTTCTAACCATCT+AGG - tig0020319:23957-23976 None:intergenic 45.0%
GCTGATGAGAATCAGGGAAA+TGG + tig0020319:24194-24213 Msa1380040:CDS 45.0%
GGTTGATTACTGCTATGGTG+TGG + tig0020319:24568-24587 Msa1380040:three_prime_UTR 45.0%
ACAATGGCGAGTGCAGGTTA+CGG + tig0020319:21157-21176 Msa1380040:exon 50.0%
TTAATCGGTGATTCCGCCGT+TGG + tig0020319:21220-21239 Msa1380040:CDS 50.0%
TGCAAGAAACGACTTCAGCG+TGG + tig0020319:21267-21286 Msa1380040:CDS 50.0%
!! GACTGATGTTGACTCGAGGT+TGG + tig0020319:21775-21794 Msa1380040:intron 50.0%
GAACCCAAGCCAACCCTAAA+TGG - tig0020319:21972-21991 None:intergenic 50.0%
!! TTAGGGTTGGCTTGGGTTCT+TGG + tig0020319:21973-21992 Msa1380040:intron 50.0%
AGCGATACAGACTGCAGAGT+AGG - tig0020319:22704-22723 None:intergenic 50.0%
CATAACTCCTCCCAACAGCT+AGG + tig0020319:22838-22857 Msa1380040:intron 50.0%
AGTAACTCCTAGCTGTTGGG+AGG - tig0020319:22848-22867 None:intergenic 50.0%
AGACCAGATAGCACTCTCAC+AGG - tig0020319:23216-23235 None:intergenic 50.0%
GGGAGAAATGGGTGAGTGTT+TGG - tig0020319:23306-23325 None:intergenic 50.0%
CTGTAAGCACACAGCCAGAA+AGG - tig0020319:23340-23359 None:intergenic 50.0%
GGCTGTGTGCTTACAGACAT+TGG + tig0020319:23344-23363 Msa1380040:intron 50.0%
ATTAGTTGCTTGCAGCGCAG+AGG - tig0020319:24108-24127 None:intergenic 50.0%
CTGGCTGCTGATGAGAATCA+GGG + tig0020319:24188-24207 Msa1380040:CDS 50.0%
ATTACCTCCGACTCCATCTC+AGG - tig0020319:24442-24461 None:intergenic 50.0%
GCAAGAACAATGGCGAGTGC+AGG + tig0020319:21151-21170 Msa1380040:exon 55.0%
! CTCACAGGATTGGCAGAGGT+AGG - tig0020319:23201-23220 None:intergenic 55.0%
! CACTCTCACAGGATTGGCAG+AGG - tig0020319:23205-23224 None:intergenic 55.0%
!! CATACTACAGGGGCGCTGTT+GGG + tig0020319:23883-23902 Msa1380040:CDS 55.0%
!! ATACTACAGGGGCGCTGTTG+GGG + tig0020319:23884-23903 Msa1380040:CDS 55.0%
GTGATCGAAGCTCGGACGTT+TGG - tig0020319:23931-23950 None:intergenic 55.0%
CCTGATTCTCATCAGCAGCC+AGG - tig0020319:24190-24209 None:intergenic 55.0%
CCTGGCTGCTGATGAGAATC+AGG + tig0020319:24187-24206 Msa1380040:CDS 55.0%
GGCAACTCAGCATCTCTGTC+CGG + tig0020319:24215-24234 Msa1380040:CDS 55.0%
GGGATTTCTTGTGCAGGACC+AGG - tig0020319:24257-24276 None:intergenic 55.0%
AAGACCTGAGATGGAGTCGG+AGG + tig0020319:24435-24454 Msa1380040:three_prime_UTR 55.0%
! GCTCAGATCTGGGACACTGC+TGG + tig0020319:21358-21377 Msa1380040:CDS 60.0%
GGGACACTGCTGGCCAAGAA+CGG + tig0020319:21368-21387 Msa1380040:CDS 60.0%
GAGCGAGGTGTGATTGACGC+AGG + tig0020319:21886-21905 Msa1380040:intron 60.0%
!! GCATACTACAGGGGCGCTGT+TGG + tig0020319:23882-23901 Msa1380040:CDS 60.0%
! GGGCGCTGTTGGGGCAATGT+TGG + tig0020319:23893-23912 Msa1380040:CDS 65.0%
GCAGCGTGCAGTACCCACAG+AGG + tig0020319:24037-24056 Msa1380040:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0020319 gene 21063 24690 21063 ID=Msa1380040;Name=Msa1380040
tig0020319 mRNA 21063 24690 21063 ID=Msa1380040-mRNA-1;Parent=Msa1380040;Name=Msa1380040-mRNA-1;_AED=0.06;_eAED=0.06;_QI=97|1|1|1|1|1|2|389|222
tig0020319 exon 21063 21389 21063 ID=Msa1380040-mRNA-1:exon:6889;Parent=Msa1380040-mRNA-1
tig0020319 exon 23863 24690 23863 ID=Msa1380040-mRNA-1:exon:6890;Parent=Msa1380040-mRNA-1
tig0020319 five_prime_UTR 21063 21159 21063 ID=Msa1380040-mRNA-1:five_prime_utr;Parent=Msa1380040-mRNA-1
tig0020319 CDS 21160 21389 21160 ID=Msa1380040-mRNA-1:cds;Parent=Msa1380040-mRNA-1
tig0020319 CDS 23863 24301 23863 ID=Msa1380040-mRNA-1:cds;Parent=Msa1380040-mRNA-1
tig0020319 three_prime_UTR 24302 24690 24302 ID=Msa1380040-mRNA-1:three_prime_utr;Parent=Msa1380040-mRNA-1
Gene Sequence

>Msa1380040

ATGGCGAGTGCAGGTTACGGTGATGCAAACCAGAAGATAGACTACGTCTTCAAGGTGGTTTTAATCGGTGATTCCGCCGTTGGAAAATCTCAGATACTAGCTCGGTTTGCAAGAAACGACTTCAGCGTGGACTCAAAAGCTACAATCGGTGTTGAGTTTCAAACTCGTACTTTGGTTATTGAACATAAGAGTGTTAAGGCTCAGATCTGGGACACTGCTGGCCAAGAACGATATAGAGCAGTTACAAGTGCATACTACAGGGGCGCTGTTGGGGCAATGTTGGTTTATGACATTACCAAACGTCCGAGCTTCGATCACATACCTAGATGGTTAGAAGAACTGCGCAACCATGCTGACAAGAATATAGTCATCATTCTTATAGGAAACAAAAGTGATCTTGAGGAGCAGCGTGCAGTACCCACAGAGGATGCAAAAGAATTTGCTGAGAAAGAAGGCTTATTTTTCTTAGAGACCTCTGCGCTGCAAGCAACTAATGTTGAGACGGCCTTCATGACTGTTTTGACAGAAATATTTAACATTGTCAACAAGAAGAACCTGGCTGCTGATGAGAATCAGGGAAATGGCAACTCAGCATCTCTGTCCGGCAAGAAGATTATTGTTCCTGGTCCTGCACAAGAAATCCCTAAGAAGAGCATGTGTTGTCAGTAA
Protein sequence

>Msa1380040

MASAGYGDANQKIDYVFKVVLIGDSAVGKSQILARFARNDFSVDSKATIGVEFQTRTLVIEHKSVKAQIWDTAGQERYRAVTSAYYRGAVGAMLVYDITKRPSFDHIPRWLEELRNHADKNIVIILIGNKSDLEEQRAVPTEDAKEFAEKEGLFFLETSALQATNVETAFMTVLTEIFNIVNKKNLAADENQGNGNSASLSGKKIIVPGPAQEIPKKSMCCQ*