AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1382590

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1382590 MtrunA17_Chr1g0155301 95.972 422 8 2 1 413 1 422 0.0 837
Msa1382590 MtrunA17_Chr5g0404321 62.353 425 139 5 1 413 1 416 0.0 534
Msa1382590 MtrunA17_Chr8g0373001 60.377 424 145 5 1 413 1 412 2.79e-178 503
Msa1382590 MtrunA17_Chr2g0286441 58.470 366 142 4 55 413 31 393 2.22e-147 424
Msa1382590 MtrunA17_Chr1g0201891 49.007 453 169 9 1 413 1 431 4.67e-146 422
Msa1382590 MtrunA17_Chr2g0278511 50.794 441 175 9 1 413 1 427 1.23e-145 421
Msa1382590 MtrunA17_Chr1g0156151 86.441 236 17 2 1 233 1 224 1.40e-137 392
Msa1382590 MtrunA17_Chr1g0196071 50.868 403 181 6 20 413 8 402 3.09e-136 395
Msa1382590 MtrunA17_Chr2g0315641 49.580 357 159 4 57 413 36 371 1.13e-121 357
Msa1382590 MtrunA17_Chr8g0373361 47.619 420 181 11 1 406 11 405 2.60e-116 345
Msa1382590 MtrunA17_Chr2g0315651 45.479 365 173 10 52 413 26 367 1.91e-95 290
Msa1382590 MtrunA17_Chr8g0373331 41.463 410 170 9 1 404 1 346 1.07e-92 283
Msa1382590 MtrunA17_Chr8g0373341 38.049 410 157 13 1 403 1 320 5.06e-61 200
Msa1382590 MtrunA17_Chr8g0373351 36.559 372 133 10 53 410 9 291 4.82e-57 189
Msa1382590 MtrunA17_Chr3g0091401 44.792 96 46 2 108 203 11 99 6.03e-17 76.3
Msa1382590 MtrunA17_Chr2g0334041 28.223 287 128 15 143 412 134 359 1.72e-14 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1382590 AT1G47270.3 61.323 393 129 6 28 413 12 388 7.01e-166 471
Msa1382590 AT1G47270.1 59.277 415 140 7 6 413 21 413 9.02e-166 471
Msa1382590 AT1G47270.2 59.179 414 140 7 7 413 21 412 9.51e-166 471
Msa1382590 AT2G18280.2 55.396 417 158 6 1 413 1 393 2.24e-156 447
Msa1382590 AT2G18280.1 55.396 417 158 6 1 413 1 393 2.24e-156 447
Msa1382590 AT2G47900.2 50.835 419 187 4 1 413 1 406 8.24e-147 423
Msa1382590 AT2G47900.1 50.835 419 187 4 1 413 1 406 8.24e-147 423
Msa1382590 AT2G47900.3 50.714 420 187 5 1 413 1 407 3.25e-145 419
Msa1382590 AT1G25280.1 49.336 452 183 8 1 413 1 445 1.30e-141 411
Msa1382590 AT1G76900.2 48.394 467 175 8 1 413 1 455 6.06e-139 405
Msa1382590 AT1G76900.1 48.394 467 175 8 1 413 1 455 6.06e-139 405
Msa1382590 AT1G76900.3 48.394 467 175 8 1 413 1 455 6.06e-139 405
Msa1382590 AT1G43640.1 45.721 444 195 8 1 413 1 429 4.35e-127 374
Msa1382590 AT3G06380.1 50.949 369 168 5 50 413 20 380 1.69e-125 368
Msa1382590 AT5G18680.1 51.220 369 160 8 55 413 31 389 6.08e-121 357
Msa1382590 AT1G53320.1 47.970 394 183 6 23 413 5 379 1.96e-120 355
Msa1382590 AT1G43640.2 49.454 366 155 5 75 413 1 363 4.19e-118 348
Msa1382590 AT3G06380.2 51.370 292 130 4 126 413 5 288 1.01e-100 301
Msa1382590 AT5G18680.2 50.820 305 131 7 118 413 3 297 3.73e-97 293
Msa1382590 AT1G25280.3 51.311 267 94 4 183 413 1 267 3.62e-85 261
Msa1382590 AT1G25280.2 51.311 267 94 4 183 413 1 267 3.62e-85 261
Msa1382590 AT1G61940.2 51.724 87 42 0 66 152 1 87 3.61e-25 103
Msa1382590 AT1G61940.1 54.098 61 28 0 66 126 1 61 9.25e-15 73.9

Find 206 sgRNAs with CRISPR-Local

Find 304 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGTATTTGCAGCTCTTTC+TGG 0.147891 tig0020520:-53259 Msa1382590:intron
AAGCCAGAAGTTGTTGAATT+TGG 0.203153 tig0020520:-52722 Msa1382590:CDS
GTGCCTCAGTTTGTAGATTA+TGG 0.216681 tig0020520:-53900 Msa1382590:CDS
GTTCTGCAGCTGAAACATTT+TGG 0.237846 tig0020520:+52543 None:intergenic
TTGAGGTTTGTGAAGATTAT+AGG 0.238744 tig0020520:+54978 None:intergenic
TCTACCTCGCAAGAAGAAAA+AGG 0.256159 tig0020520:+51003 Msa1382580:CDS
TAGGAGCAGTTCCTCCTTCT+TGG 0.256813 tig0020520:+52798 None:intergenic
GGACAGAACAAAACTTAATT+TGG 0.258161 tig0020520:+55124 None:intergenic
TTCAATGGGGACTTCATCTT+TGG 0.269120 tig0020520:-51066 Msa1382590:three_prime_UTR
TTGTGTAATGCTAGCTGTTT+AGG 0.272202 tig0020520:-52288 Msa1382590:three_prime_UTR
TCCTTCTTGGACTGAAGATA+TGG 0.272539 tig0020520:+52811 None:intergenic
GTCTTTATCGTCGTGGTTGA+TGG 0.275216 tig0020520:-51880 Msa1382590:three_prime_UTR
GAGCCAAATTCAACAACTTC+TGG 0.280199 tig0020520:+52719 None:intergenic
ATTACCTGCTTCAGTGATAT+AGG 0.293400 tig0020520:+53820 None:intergenic
AATAATTCACTCATGTTTCA+TGG 0.298043 tig0020520:+51798 Msa1382580:CDS
GATAAGTTGTGTATATTTGT+AGG 0.300528 tig0020520:+54206 None:intergenic
TCTCTTTCTCTTTAGTTTGA+AGG 0.302700 tig0020520:-51206 Msa1382590:three_prime_UTR
TGACATTGCACATAATAAAA+AGG 0.306400 tig0020520:-52180 Msa1382590:three_prime_UTR
TGATGTTGTCAACACTTTCT+TGG 0.313117 tig0020520:-52134 Msa1382590:three_prime_UTR
TGCTGCAACTTGCAACTTGA+TGG 0.318405 tig0020520:+52965 None:intergenic
TCCACATATAGTTTATATCT+TGG 0.326644 tig0020520:-53632 Msa1382590:CDS
AAGGGTCAATCCACATTCAT+AGG 0.332387 tig0020520:+51640 Msa1382580:CDS
CGCTCTAGGGCCGAAGGATA+TGG 0.340717 tig0020520:-50880 Msa1382590:three_prime_UTR
CACTGAATTGGATTGTCTCT+TGG 0.352418 tig0020520:+53680 None:intergenic
CTCTTAATAAAACACTGAAT+TGG 0.367629 tig0020520:+53668 None:intergenic
TCTAGAAAATGAGGAAATCT+TGG 0.369298 tig0020520:+54852 None:intergenic
TTTGTCCAAGACATTGCCTT+TGG 0.372742 tig0020520:-54930 Msa1382590:five_prime_UTR
ATCTTTGGTTCTGCTTCACT+TGG 0.374825 tig0020520:-51051 Msa1382590:three_prime_UTR
CAGCCAACAGTTCGCCGTTA+TGG 0.376958 tig0020520:+51957 Msa1382580:CDS
TTGGAAGTACTTGCTTCATA+TGG 0.387123 tig0020520:+52936 None:intergenic
CAGCTAGCATTACACAAACT+TGG 0.390221 tig0020520:+52294 None:intergenic
GTGAGAGTGGATGGAGGTTG+AGG 0.390805 tig0020520:+54961 None:intergenic
ATACTTACATTGGAAAGTTA+AGG 0.392067 tig0020520:-53135 Msa1382590:intron
CTCTTCGGACTCAGTCTTGT+AGG 0.397966 tig0020520:-51664 Msa1382590:three_prime_UTR
TTCATGCTCATTGTGGCAAT+TGG 0.398540 tig0020520:+52760 None:intergenic
GTTGGTGCTGATAGTGATAT+TGG 0.400859 tig0020520:+54048 None:intergenic
ATGTCTTGGACAAAACAAAA+AGG 0.404771 tig0020520:+54939 None:intergenic
GGTTCTGCTTCACTTGGAAC+CGG 0.405060 tig0020520:-51045 Msa1382590:three_prime_UTR
TTACCTGCTTCAGTGATATA+GGG 0.408155 tig0020520:+53821 None:intergenic
CATTGCCTTTGGAGTATTTG+AGG 0.412801 tig0020520:-54919 Msa1382590:five_prime_UTR
GGAAAGGCACCAATGCAAAA+AGG 0.413609 tig0020520:+51465 Msa1382580:CDS
ACCAAGTTCACGATCTTAGA+TGG 0.416091 tig0020520:-53013 Msa1382590:CDS
AGGAAATCTTGGAGAGAACT+TGG 0.419492 tig0020520:+54863 None:intergenic
TGTGCCTGAAGAAGATGCTT+TGG 0.420059 tig0020520:-51304 Msa1382590:three_prime_UTR
CATGCCAAAGCATCTTCTTC+AGG 0.430172 tig0020520:+51300 Msa1382580:CDS
CATCATCATCAGAGAGTCAA+TGG 0.430216 tig0020520:-54014 Msa1382590:CDS
TTTGTTCTGTCCATGTGGTT+TGG 0.430653 tig0020520:-55113 Msa1382590:five_prime_UTR
ATCTCCTTGGACCTGGACCC+CGG 0.433688 tig0020520:+50924 Msa1382580:CDS
GTGTGCTTAGAAAGATTCTC+TGG 0.434313 tig0020520:-51108 Msa1382590:three_prime_UTR
AAGGGAAAAGGAAAATGAAA+AGG 0.436087 tig0020520:+51443 Msa1382580:CDS
TGAGTTTCCAAAGGGGACTT+TGG 0.437954 tig0020520:-51174 Msa1382590:three_prime_UTR
TGCAGTTGTTCATGCCATCT+TGG 0.442171 tig0020520:+52647 None:intergenic
GATGATGATGATGGTGTTGT+TGG 0.443322 tig0020520:+54030 None:intergenic
CCTTCTTGGACTGAAGATAT+GGG 0.443500 tig0020520:+52812 None:intergenic
GTTGCTACTTTATAGTTAGC+AGG 0.444061 tig0020520:+52905 None:intergenic
TTGTGTTGGATTATAGCTTA+AGG 0.446378 tig0020520:-55232 Msa1382590:five_prime_UTR
GAGTATTGTGAGAGAGATGA+AGG 0.449992 tig0020520:-54169 Msa1382590:CDS
ACCATCTAAGATCGTGAACT+TGG 0.451643 tig0020520:+53012 None:intergenic
ATGTGGTTTGGTTGTAAAGA+AGG 0.456396 tig0020520:-55101 Msa1382590:five_prime_UTR
GGGGTCCAGGTCCAAGGAGA+TGG 0.458939 tig0020520:-50922 Msa1382590:three_prime_UTR
AAGGAGATGAAAGAGGGAAT+TGG 0.461217 tig0020520:-54150 Msa1382590:CDS
AATAACACCAAAGTCCCCTT+TGG 0.463540 tig0020520:+51167 Msa1382580:CDS
CATAACGGCGAACTGTTGGC+TGG 0.467257 tig0020520:-51956 Msa1382590:three_prime_UTR
CTTTGGCATGTTGATTCGGT+TGG 0.469572 tig0020520:-51287 Msa1382590:three_prime_UTR
TCGTGGTTGATGGTTTCTAC+AGG 0.474450 tig0020520:-51870 Msa1382590:three_prime_UTR
GATATTGGTGAGGAACATTC+TGG 0.475287 tig0020520:+54063 None:intergenic
TTTCCCTTTCCTCGCTCGAT+GGG 0.476396 tig0020520:-51428 Msa1382590:three_prime_UTR
CGCTCTAGGGCCGAAGGAGA+TGG 0.477305 tig0020520:-50838 Msa1382590:three_prime_UTR
TGTCACATCAATGCTCTCTT+CGG 0.477554 tig0020520:-51679 Msa1382590:three_prime_UTR
GATGCTTTGGCATGTTGATT+CGG 0.479473 tig0020520:-51291 Msa1382590:three_prime_UTR
CAATGGAACATGGTGAGGTC+GGG 0.480101 tig0020520:+51742 Msa1382580:CDS
ATTGGATTGTCTCTTGGAGC+AGG 0.482570 tig0020520:+53686 None:intergenic
TTACACAAACTTGGGCCTGC+AGG 0.483485 tig0020520:+52303 None:intergenic
AGCATCGTCCACCATCTCCT+TGG 0.484267 tig0020520:+50911 Msa1382580:CDS
GGGACGGGTTACAGTGGCTT+CGG 0.485242 tig0020520:-52603 Msa1382590:CDS
AGGAACTGCTCCTACTCCTA+TGG 0.485778 tig0020520:-52789 Msa1382590:CDS
GAGATGAAGGAGATGAAAGA+GGG 0.489271 tig0020520:-54156 Msa1382590:CDS
GTGTTTGAATTTCAAGGGAC+GGG 0.490017 tig0020520:-52618 Msa1382590:CDS
AGGAGTTATCATTCACAAGC+TGG 0.492801 tig0020520:+52401 None:intergenic
TGTCCCATCGAGCGAGGAAA+GGG 0.494803 tig0020520:+51425 Msa1382580:CDS
TCTGAATCAGATAAGAAAGA+AGG 0.496204 tig0020520:+51621 Msa1382580:CDS
AACACTTTCTTGGTGGAAAG+TGG 0.497925 tig0020520:-52124 Msa1382590:three_prime_UTR
AGTTGCAATATTTCTGTACA+AGG 0.499276 tig0020520:-52066 Msa1382590:three_prime_UTR
ATCGAGCGAGGAAAGGGAAA+AGG 0.501898 tig0020520:+51431 Msa1382580:CDS
AAGTTGCAAGTTGCAGCAAA+AGG 0.503666 tig0020520:-52960 Msa1382590:CDS
TGATATGAGCAAACTTCTCT+TGG 0.505017 tig0020520:-53236 Msa1382590:CDS
AGTGCAACCAACACAACCTA+TGG 0.506396 tig0020520:+51533 Msa1382580:CDS
TGCTCCGGGGTCCAGGTCCA+AGG 0.507731 tig0020520:-50928 Msa1382590:three_prime_UTR
TTATATCTTGGTCTAAGCCC+TGG 0.508714 tig0020520:-53620 Msa1382590:intron
AGAGATGAAGGAGATGAAAG+AGG 0.511465 tig0020520:-54157 Msa1382590:CDS
AGACACTTGAACAGTGTGGC+TGG 0.511733 tig0020520:-53852 Msa1382590:CDS
GGAAGTGTAACTTGAACTCC+AGG 0.513147 tig0020520:-51840 Msa1382590:three_prime_UTR
CACAAAGAGGGGAGGGAATG+TGG 0.514278 tig0020520:+54890 None:intergenic
AGATTCTCTGGTTGTGACAC+GGG 0.514523 tig0020520:-51096 Msa1382590:three_prime_UTR
TAGTTAGCAGGAGCAGCCCT+TGG 0.515473 tig0020520:+52917 None:intergenic
GAAGATGTCAATGTCTGATC+CGG 0.516487 tig0020520:+51026 Msa1382580:CDS
ACTATTAGACATAATTCAAA+GGG 0.516941 tig0020520:-53971 Msa1382590:CDS
ACCAAGATATAAACTATATG+TGG 0.517704 tig0020520:+53631 None:intergenic
GGAACATGGTGAGGTCGGGC+AGG 0.517884 tig0020520:+51746 Msa1382580:CDS
ATTGTACTCCGCATTCGCCT+TGG 0.518756 tig0020520:+52845 None:intergenic
GGTGTTTGAATTTCAAGGGA+CGG 0.518832 tig0020520:-52619 Msa1382590:CDS
AGGGAAAAGGAAAATGAAAA+GGG 0.518930 tig0020520:+51444 Msa1382580:CDS
TTGTCCTCCATAGGTTGTGT+TGG 0.520819 tig0020520:-51540 Msa1382590:three_prime_UTR
GATATCAAAGAAGATTTCAA+AGG 0.522974 tig0020520:+52381 None:intergenic
ACTTCCTTTGAGTTTCCAAA+GGG 0.524701 tig0020520:-51182 Msa1382590:three_prime_UTR
GCATTATCCACCATCTCCTT+CGG 0.525548 tig0020520:+50828 Msa1382580:CDS
GCATCATCCACCATATCCTT+CGG 0.526033 tig0020520:+50870 Msa1382580:CDS
TTATGGAGAGACATAACAAA+GGG 0.526760 tig0020520:-53883 Msa1382590:CDS
ATACTCCAAAGGCAATGTCT+TGG 0.529963 tig0020520:+54925 None:intergenic
AGCATTGATGTGACAACAGA+TGG 0.531129 tig0020520:+51687 Msa1382580:CDS
AGAGCTAACAATACTTACAT+TGG 0.531671 tig0020520:-53145 Msa1382590:CDS
TGGCAATTGGTAAATTCCAT+AGG 0.538026 tig0020520:+52773 None:intergenic
GTCCACCATCTCCTTGGACC+TGG 0.539700 tig0020520:+50917 Msa1382580:CDS
TCAATGGAACATGGTGAGGT+CGG 0.539979 tig0020520:+51741 Msa1382580:CDS
TTCAGTGTCATAACACTGAC+TGG 0.540460 tig0020520:+51567 Msa1382580:CDS
AACTTTCAAAACAAAGCTTG+TGG 0.541276 tig0020520:+55020 None:intergenic
TTTCCCTATATCACTGAAGC+AGG 0.541444 tig0020520:-53824 Msa1382590:intron
ATGTTGGTGTTTGAATTTCA+AGG 0.542716 tig0020520:-52624 Msa1382590:CDS
TTGGTAAATTCCATAGGAGT+AGG 0.543440 tig0020520:+52779 None:intergenic
TCAATCAATGAAAGAGACCC+TGG 0.549089 tig0020520:+51822 Msa1382580:CDS
CGACCATAACGGCGAACTGT+TGG 0.551046 tig0020520:-51960 Msa1382590:three_prime_UTR
CAATGAGGAAGAAGACTCAA+TGG 0.551065 tig0020520:+51725 Msa1382580:CDS
AGACAAGAGTCTAGAAAATG+AGG 0.552127 tig0020520:+54843 None:intergenic
AACTTCCTTTGAGTTTCCAA+AGG 0.552524 tig0020520:-51183 Msa1382590:three_prime_UTR
TACTATTAGACATAATTCAA+AGG 0.554134 tig0020520:-53972 Msa1382590:CDS
AGCTAGCATTACACAAACTT+GGG 0.554445 tig0020520:+52295 None:intergenic
TACACAAACTTGGGCCTGCA+GGG 0.554604 tig0020520:+52304 None:intergenic
ATATGAAGCAAGTACTTCCA+AGG 0.555692 tig0020520:-52934 Msa1382590:CDS
TGTTGTCAACACTTTCTTGG+TGG 0.557651 tig0020520:-52131 Msa1382590:three_prime_UTR
TACCATGCAAGATATTGTGT+TGG 0.558830 tig0020520:-55246 Msa1382590:five_prime_UTR
GGACTGAGTTTAACATTAGT+CGG 0.558841 tig0020520:+51916 Msa1382580:CDS
GTCCAGGTCCAAGGAGATGG+TGG 0.560062 tig0020520:-50919 Msa1382590:three_prime_UTR
ATTATGTCTAATAGTAACTC+AGG 0.562516 tig0020520:+53979 None:intergenic
TGTTGGTGTTTGAATTTCAA+GGG 0.562671 tig0020520:-52623 Msa1382590:CDS
TTGTCCCATCGAGCGAGGAA+AGG 0.563695 tig0020520:+51424 Msa1382580:CDS
ATCATCATCAGAGAGTCAAT+GGG 0.565599 tig0020520:-54013 Msa1382590:CDS
GAACTTGGAAAGCACAAAGA+GGG 0.569713 tig0020520:+54878 None:intergenic
TATGGTCGCAAGTTCAGTTG+TGG 0.571661 tig0020520:+51975 Msa1382580:CDS
AAAGGAAAATGAAAAGGGAA+AGG 0.572297 tig0020520:+51449 Msa1382580:CDS
CTGAATCAGATAAGAAAGAA+GGG 0.574236 tig0020520:+51622 Msa1382580:CDS
TTTCCACATCAAACTCTAGT+GGG 0.574624 tig0020520:+51379 Msa1382580:CDS
AACTGAACTTGCGACCATAA+CGG 0.579458 tig0020520:-51971 Msa1382590:three_prime_UTR
AGGGTCAATCCACATTCATA+GGG 0.580060 tig0020520:+51641 Msa1382580:CDS
GCTACAAGTGCTCGGCGAGA+TGG 0.582646 tig0020520:+53925 None:intergenic
TTGCAGATTCATGCTCATTG+TGG 0.583392 tig0020520:+52753 None:intergenic
TCTCTCACAATACTCTTGAA+AGG 0.583510 tig0020520:+54177 None:intergenic
AAGATTCTCTGGTTGTGACA+CGG 0.584236 tig0020520:-51097 Msa1382590:three_prime_UTR
CCCATATCTTCAGTCCAAGA+AGG 0.587259 tig0020520:-52812 Msa1382590:CDS
TCTTGTAGGCCCTATGAATG+TGG 0.587297 tig0020520:-51650 Msa1382590:three_prime_UTR
TTCTTTACAACCAAACCACA+TGG 0.588770 tig0020520:+55103 None:intergenic
TTTCCCACTAGAGTTTGATG+TGG 0.593804 tig0020520:-51382 Msa1382590:three_prime_UTR
ATCCAACACAATATCTTGCA+TGG 0.596382 tig0020520:+55244 None:intergenic
CTCAATGTTCTTCGTACGAG+AGG 0.596421 tig0020520:-52869 Msa1382590:CDS
AAGAATTGTCCCATCGAGCG+AGG 0.597091 tig0020520:+51419 Msa1382580:CDS
GGCATGAACAACTGCAATGT+TGG 0.599403 tig0020520:-52640 Msa1382590:CDS
GAGAGGATATCGATAATCCA+TGG 0.599763 tig0020520:+52472 None:intergenic
CTTCTTGGACTGAAGATATG+GGG 0.599973 tig0020520:+52813 None:intergenic
CGAGAGGTCCAAGGCGAATG+CGG 0.601851 tig0020520:-52853 Msa1382590:CDS
AGCATCTTCTTCAGGCACAA+AGG 0.605435 tig0020520:+51308 Msa1382580:CDS
ATAAAGACACTCACAATCGC+AGG 0.606983 tig0020520:+51895 Msa1382580:CDS
ACAAAGGATAAGAAGCATCA+TGG 0.606998 tig0020520:+51324 Msa1382580:CDS
ATTAGACATAATTCAAAGGG+TGG 0.608482 tig0020520:-53968 Msa1382590:CDS
TGGAAAGCACAAAGAGGGGA+GGG 0.611473 tig0020520:+54883 None:intergenic
GATTCTCTGGTTGTGACACG+GGG 0.616837 tig0020520:-51095 Msa1382590:three_prime_UTR
TTCTTCGTACGAGAGGTCCA+AGG 0.616949 tig0020520:-52862 Msa1382590:CDS
ACTAAGCATATGCATAGACA+TGG 0.617362 tig0020520:-54102 Msa1382590:CDS
AGTCCCCTTTGGAAACTCAA+AGG 0.618613 tig0020520:+51178 Msa1382580:CDS
TACAAGAAAATATATGCTTG+AGG 0.619008 tig0020520:+52230 None:intergenic
ACACTGACTGGTGACAATAG+AGG 0.621058 tig0020520:+51579 Msa1382580:CDS
TCTTGCATGGTATTTAAGTG+TGG 0.625775 tig0020520:+55257 None:intergenic
AAGTACCTCAAATACTCCAA+AGG 0.627238 tig0020520:+54914 None:intergenic
AGAGGAGCAACAATGCATGT+TGG 0.629716 tig0020520:+51597 Msa1382580:CDS
GCTGCTCAAACAGATTGCAA+AGG 0.630739 tig0020520:+52436 None:intergenic
GCAACCAACACAACCTATGG+AGG 0.633233 tig0020520:+51536 Msa1382580:CDS
AGGCAAGTGATAAAAGTGCA+AGG 0.635649 tig0020520:-55212 Msa1382590:five_prime_UTR
TGTGGTTTGGTTGTAAAGAA+GGG 0.635990 tig0020520:-55100 Msa1382590:five_prime_UTR
ATTGGCAACATGTATAGAAG+AGG 0.636703 tig0020520:-54132 Msa1382590:CDS
CGGGAAGGACATATTCACCA+TGG 0.637363 tig0020520:-52489 Msa1382590:CDS
TGGAAGCTAGTGAAACCACT+TGG 0.639315 tig0020520:-53948 Msa1382590:CDS
TTTCAAGGGACGGGTTACAG+TGG 0.641025 tig0020520:-52609 Msa1382590:CDS
CTAACCATGAAGAAAACACT+CGG 0.642497 tig0020520:+50971 Msa1382580:CDS
AGACTCAATGGAACATGGTG+AGG 0.644780 tig0020520:+51737 Msa1382580:CDS
AGGCACAAGCTACAAGTGCT+CGG 0.645228 tig0020520:+53917 None:intergenic
AGAACTTGGAAAGCACAAAG+AGG 0.645315 tig0020520:+54877 None:intergenic
AACTTGGAAAGCACAAAGAG+GGG 0.645714 tig0020520:+54879 None:intergenic
TATGAAGCAAGTACTTCCAA+GGG 0.646074 tig0020520:-52933 Msa1382590:CDS
TTGGAAAGCACAAAGAGGGG+AGG 0.646454 tig0020520:+54882 None:intergenic
GAAGAAGACTCAATGGAACA+TGG 0.649274 tig0020520:+51732 Msa1382580:CDS
TCTAGGGCCGAAGGAGATGG+TGG 0.656152 tig0020520:-50835 Msa1382590:three_prime_UTR
TCTAGGGCCGAAGGATATGG+TGG 0.657238 tig0020520:-50877 Msa1382590:three_prime_UTR
TGTGAGTGTCTTTATCGTCG+TGG 0.661271 tig0020520:-51887 Msa1382590:three_prime_UTR
GATGTGACAACAGATGGAGA+AGG 0.661909 tig0020520:+51693 Msa1382580:CDS
ATGTCTAATAGTAACTCAGG+AGG 0.663267 tig0020520:+53982 None:intergenic
AAGGCTTGAAAAGCAGAGAG+AGG 0.664732 tig0020520:+52455 None:intergenic
ATTATGGAGAGACATAACAA+AGG 0.674047 tig0020520:-53884 Msa1382590:CDS
ATATCTTCAGTCCAAGAAGG+AGG 0.675556 tig0020520:-52809 Msa1382590:CDS
TCTCCATAATCTACAAACTG+AGG 0.678466 tig0020520:+53897 None:intergenic
TGCTCGGCGAGATGGCCAAG+TGG 0.681754 tig0020520:+53933 None:intergenic
CTTCCTTTGAGTTTCCAAAG+GGG 0.694307 tig0020520:-51181 Msa1382590:three_prime_UTR
AGAAGGAAACACTAACAATG+AGG 0.700109 tig0020520:+51710 Msa1382580:CDS
TGCTGATAGTGATATTGGTG+AGG 0.701371 tig0020520:+54053 None:intergenic
GATGGAAGCGAAGAAACATG+TGG 0.713919 tig0020520:+52983 None:intergenic
AGGTTGTGTTGGTTGCACTG+AGG 0.746719 tig0020520:-51529 Msa1382590:three_prime_UTR
GAAGTGTAACTTGAACTCCA+GGG 0.753989 tig0020520:-51839 Msa1382590:three_prime_UTR
GGAAGCGAAGAAACATGTGG+AGG 0.757377 tig0020520:+52986 None:intergenic
GTTAAGACACTTGAACAGTG+TGG 0.771470 tig0020520:-53856 Msa1382590:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTAAATTCATATTAGATTA+AGG - tig0020520:51321-51340 Msa1382590:three_prime_UTR 15.0%
!!! TTTTGTAATAAATATTGTGA+TGG + tig0020520:51367-51386 Msa1382580:CDS 15.0%
!! ATCACAATATTTATTACAAA+AGG - tig0020520:51366-51385 Msa1382590:three_prime_UTR 15.0%
!! TCACAATATTTATTACAAAA+GGG - tig0020520:51367-51386 Msa1382590:three_prime_UTR 15.0%
!!! TTTTCTTTCACTTCTTTTTT+TGG - tig0020520:51288-51307 Msa1382590:three_prime_UTR 20.0%
!! GATATAGCAGTAAAAAAATA+AGG - tig0020520:51389-51408 Msa1382590:three_prime_UTR 20.0%
!!! AACTGCCTTAATTATTTTTT+GGG + tig0020520:51584-51603 Msa1382580:CDS 20.0%
!!! AAACTGCCTTAATTATTTTT+TGG + tig0020520:51585-51604 Msa1382580:CDS 20.0%
!!! GCAGTTTTTTTTTTTTTTGA+GGG - tig0020520:51598-51617 Msa1382590:three_prime_UTR 20.0%
!!! TTTTTTGAGGGAAATAATTA+AGG - tig0020520:51610-51629 Msa1382590:three_prime_UTR 20.0%
!!! TTTTTCTTGTTCCTTTTTAT+AGG + tig0020520:51659-51678 Msa1382580:CDS 20.0%
!! TACTATTAGACATAATTCAA+AGG - tig0020520:52102-52121 Msa1382590:three_prime_UTR 20.0%
!! ACTATTAGACATAATTCAAA+GGG - tig0020520:52103-52122 Msa1382590:three_prime_UTR 20.0%
!!! TGTAATTTTTGTTTTAAGCT+TGG - tig0020520:52308-52327 Msa1382590:three_prime_UTR 20.0%
!! ACATGAAATTATTCTCTATT+TGG - tig0020520:52493-52512 Msa1382590:CDS 20.0%
!!! TTTGGTAACAAAATTTTGTT+TGG - tig0020520:52511-52530 Msa1382590:CDS 20.0%
!! AAAAAAAAAAGCCTAAAAGA+AGG + tig0020520:52607-52626 None:intergenic 20.0%
!!! TTTTTTTAGTCTTTGAACTA+TGG - tig0020520:52715-52734 Msa1382590:CDS 20.0%
!! AATAACACAATTCACAAATA+TGG + tig0020520:52798-52817 None:intergenic 20.0%
!!! CACAGAATTTTTAATATCAT+TGG - tig0020520:52889-52908 Msa1382590:CDS 20.0%
!! TAATCATTAAGCAAAATAAC+AGG - tig0020520:53023-53042 Msa1382590:CDS 20.0%
!! GAAAAAGTTATACTACAATT+TGG - tig0020520:53556-53575 Msa1382590:intron 20.0%
!! ATACTACAATTTGGAAAAAT+CGG - tig0020520:53565-53584 Msa1382590:intron 20.0%
!!! TTTTGTGTTTCTTACTTTTT+TGG - tig0020520:54830-54849 Msa1382590:intron 20.0%
! CAAGACCCAAAAAATAATTA+AGG - tig0020520:51576-51595 Msa1382590:three_prime_UTR 25.0%
!!! GGCAGTTTTTTTTTTTTTTG+AGG - tig0020520:51597-51616 Msa1382590:three_prime_UTR 25.0%
! GATAAGTTGTGTATATTTGT+AGG + tig0020520:51871-51890 Msa1382580:CDS 25.0%
! ATTATGTCTAATAGTAACTC+AGG + tig0020520:52098-52117 None:intergenic 25.0%
! CTCTTAATAAAACACTGAAT+TGG + tig0020520:52409-52428 None:intergenic 25.0%
!!! AGTGTTTTATTAAGAGAGAA+AGG - tig0020520:52413-52432 Msa1382590:CDS 25.0%
!!! GTGTTTTATTAAGAGAGAAA+GGG - tig0020520:52414-52433 Msa1382590:CDS 25.0%
! TCCACATATAGTTTATATCT+TGG - tig0020520:52442-52461 Msa1382590:CDS 25.0%
! ACCAAGATATAAACTATATG+TGG + tig0020520:52446-52465 None:intergenic 25.0%
!! AACTTGAATTACCTTCTTTT+AGG - tig0020520:52593-52612 Msa1382590:CDS 25.0%
!!! ATTGATTTGTAAGTTTACTC+TGG + tig0020520:52664-52683 None:intergenic 25.0%
! GTTCAAAGACTAAAAAAAGA+AGG + tig0020520:52713-52732 None:intergenic 25.0%
! ATACTTACATTGGAAAGTTA+AGG - tig0020520:52939-52958 Msa1382590:CDS 25.0%
!! AATAACAGGTCTAATTTTCT+CGG - tig0020520:53037-53056 Msa1382590:intron 25.0%
! TACTACAATTTGGAAAAATC+GGG - tig0020520:53566-53585 Msa1382590:intron 25.0%
! GATATCAAAGAAGATTTCAA+AGG + tig0020520:53696-53715 None:intergenic 25.0%
! TACAAGAAAATATATGCTTG+AGG + tig0020520:53847-53866 None:intergenic 25.0%
! TGACATTGCACATAATAAAA+AGG - tig0020520:53894-53913 Msa1382590:CDS 25.0%
!!! TGTATTTTTATGGTGTACAA+GGG - tig0020520:54031-54050 Msa1382590:CDS 25.0%
! AATAATTCACTCATGTTTCA+TGG + tig0020520:54279-54298 None:intergenic 25.0%
! CACAAAAACAAAATTAGATG+AGG + tig0020520:54949-54968 None:intergenic 25.0%
TTGTGTTGGATTATAGCTTA+AGG - tig0020520:50842-50861 Msa1382590:three_prime_UTR 30.0%
GGACAGAACAAAACTTAATT+TGG + tig0020520:50953-50972 Msa1382580:CDS 30.0%
AACTTTCAAAACAAAGCTTG+TGG + tig0020520:51057-51076 Msa1382580:CDS 30.0%
TTGAGGTTTGTGAAGATTAT+AGG + tig0020520:51099-51118 Msa1382580:CDS 30.0%
ATGTCTTGGACAAAACAAAA+AGG + tig0020520:51138-51157 Msa1382580:CDS 30.0%
TCTAGAAAATGAGGAAATCT+TGG + tig0020520:51225-51244 Msa1382580:CDS 30.0%
AGAAAAGCTCAAAAATTGAG+AGG + tig0020520:51274-51293 Msa1382580:CDS 30.0%
!! GCAGTTGAAAATTTTGAAAG+AGG - tig0020520:51632-51651 Msa1382590:three_prime_UTR 30.0%
TTGAAAGAGGTCCTATAAAA+AGG - tig0020520:51645-51664 Msa1382590:three_prime_UTR 30.0%
ACACTAACTACATTGAACTT+AGG - tig0020520:51754-51773 Msa1382590:three_prime_UTR 30.0%
GTGTTTGAAAGAGAGAAATT+AGG + tig0020520:51825-51844 Msa1382580:CDS 30.0%
TTTCTCTCTTTCAAACACAA+TGG - tig0020520:51827-51846 Msa1382590:three_prime_UTR 30.0%
ATTAGACATAATTCAAAGGG+TGG - tig0020520:52106-52125 Msa1382590:three_prime_UTR 30.0%
ATTATGGAGAGACATAACAA+AGG - tig0020520:52190-52209 Msa1382590:three_prime_UTR 30.0%
TTATGGAGAGACATAACAAA+GGG - tig0020520:52191-52210 Msa1382590:three_prime_UTR 30.0%
CACAAATCGAGAATAGTTAA+CGG + tig0020520:52757-52776 None:intergenic 30.0%
! TAACTATTCTCGATTTGTGA+AGG - tig0020520:52758-52777 Msa1382590:CDS 30.0%
AGAGCTAACAATACTTACAT+TGG - tig0020520:52929-52948 Msa1382590:CDS 30.0%
! AATCAATCCACATCTTTTCA+TGG - tig0020520:52973-52992 Msa1382590:CDS 30.0%
!!! GCTTTGTTTTTCAAAATCAG+AGG + tig0020520:53406-53425 None:intergenic 30.0%
! ATGTTGGTGTTTGAATTTCA+AGG - tig0020520:53450-53469 Msa1382590:intron 30.0%
! TGTTGGTGTTTGAATTTCAA+GGG - tig0020520:53451-53470 Msa1382590:intron 30.0%
! AGTTGCAATATTTCTGTACA+AGG - tig0020520:54008-54027 Msa1382590:CDS 30.0%
!! CTGTACAAGGTGTATTTTTA+TGG - tig0020520:54021-54040 Msa1382590:CDS 30.0%
!! GTGTATTTTTATGGTGTACA+AGG - tig0020520:54030-54049 Msa1382590:CDS 30.0%
CTGAATCAGATAAGAAAGAA+GGG + tig0020520:54455-54474 None:intergenic 30.0%
TCTGAATCAGATAAGAAAGA+AGG + tig0020520:54456-54475 None:intergenic 30.0%
! TCTTTGATACCTTTTTGCAT+TGG - tig0020520:54600-54619 Msa1382590:intron 30.0%
AAAGGAAAATGAAAAGGGAA+AGG + tig0020520:54628-54647 None:intergenic 30.0%
AGGGAAAAGGAAAATGAAAA+GGG + tig0020520:54633-54652 None:intergenic 30.0%
AAGGGAAAAGGAAAATGAAA+AGG + tig0020520:54634-54653 None:intergenic 30.0%
!! TCTCTTTCTCTTTAGTTTGA+AGG - tig0020520:54868-54887 Msa1382590:five_prime_UTR 30.0%
! GCAAAAAAATGAGGTGTTAA+AGG + tig0020520:55129-55148 None:intergenic 30.0%
TCTTGCATGGTATTTAAGTG+TGG + tig0020520:50820-50839 Msa1382580:CDS 35.0%
TACCATGCAAGATATTGTGT+TGG - tig0020520:50828-50847 Msa1382590:three_prime_UTR 35.0%
ATCCAACACAATATCTTGCA+TGG + tig0020520:50833-50852 Msa1382580:CDS 35.0%
!!! TAAGTTTTGTTCTGTCCATG+TGG - tig0020520:50956-50975 Msa1382590:three_prime_UTR 35.0%
TTCTTTACAACCAAACCACA+TGG + tig0020520:50974-50993 Msa1382580:CDS 35.0%
!! ATGTGGTTTGGTTGTAAAGA+AGG - tig0020520:50973-50992 Msa1382590:three_prime_UTR 35.0%
!! TGTGGTTTGGTTGTAAAGAA+GGG - tig0020520:50974-50993 Msa1382590:three_prime_UTR 35.0%
ACAAAACAAAAAGGTGAGAG+TGG + tig0020520:51129-51148 Msa1382580:CDS 35.0%
AAGTACCTCAAATACTCCAA+AGG + tig0020520:51163-51182 Msa1382580:CDS 35.0%
AGACAAGAGTCTAGAAAATG+AGG + tig0020520:51234-51253 Msa1382580:CDS 35.0%
! TTTTCTAGACTCTTGTCTCA+CGG - tig0020520:51236-51255 Msa1382590:three_prime_UTR 35.0%
! CATTGATTACACAGCTTTCA+AGG - tig0020520:51502-51521 Msa1382590:three_prime_UTR 35.0%
AGAAAAATCTCAAGAGAAGC+AGG - tig0020520:51672-51691 Msa1382590:three_prime_UTR 35.0%
TCTCTCACAATACTCTTGAA+AGG + tig0020520:51900-51919 Msa1382580:CDS 35.0%
ATTGGCAACATGTATAGAAG+AGG - tig0020520:51942-51961 Msa1382590:three_prime_UTR 35.0%
ACTAAGCATATGCATAGACA+TGG - tig0020520:51972-51991 Msa1382590:three_prime_UTR 35.0%
ATCATCATCAGAGAGTCAAT+GGG - tig0020520:52061-52080 Msa1382590:three_prime_UTR 35.0%
ATGTCTAATAGTAACTCAGG+AGG + tig0020520:52095-52114 None:intergenic 35.0%
TCTCCATAATCTACAAACTG+AGG + tig0020520:52180-52199 None:intergenic 35.0%
TTACCTGCTTCAGTGATATA+GGG + tig0020520:52256-52275 None:intergenic 35.0%
ATTACCTGCTTCAGTGATAT+AGG + tig0020520:52257-52276 None:intergenic 35.0%
!! GTTTTAAGCTTGGAAGAACA+TGG - tig0020520:52318-52337 Msa1382590:three_prime_UTR 35.0%
ATTGTATTTGCAGCTCTTTC+TGG - tig0020520:52815-52834 Msa1382590:CDS 35.0%
TGATATGAGCAAACTTCTCT+TGG - tig0020520:52838-52857 Msa1382590:CDS 35.0%
! GAAAAGATGTGGATTGATTG+AGG + tig0020520:52972-52991 None:intergenic 35.0%
GCATTAACCATGAAAAGATG+TGG + tig0020520:52983-53002 None:intergenic 35.0%
!! TTGGAAGTACTTGCTTCATA+TGG + tig0020520:53141-53160 None:intergenic 35.0%
ATATGAAGCAAGTACTTCCA+AGG - tig0020520:53140-53159 Msa1382590:CDS 35.0%
TATGAAGCAAGTACTTCCAA+GGG - tig0020520:53141-53160 Msa1382590:CDS 35.0%
!! GTTGCTACTTTATAGTTAGC+AGG + tig0020520:53172-53191 None:intergenic 35.0%
TTGGTAAATTCCATAGGAGT+AGG + tig0020520:53298-53317 None:intergenic 35.0%
TGGCAATTGGTAAATTCCAT+AGG + tig0020520:53304-53323 None:intergenic 35.0%
AAGCCAGAAGTTGTTGAATT+TGG - tig0020520:53352-53371 Msa1382590:intron 35.0%
! TGAAAAACAAAGCACCAAGA+TGG - tig0020520:53413-53432 Msa1382590:intron 35.0%
ACAATTTGGAAAAATCGGGA+AGG - tig0020520:53570-53589 Msa1382590:intron 35.0%
AGCTAGCATTACACAAACTT+GGG + tig0020520:53782-53801 None:intergenic 35.0%
TTGTGTAATGCTAGCTGTTT+AGG - tig0020520:53786-53805 Msa1382590:intron 35.0%
TGATGTTGTCAACACTTTCT+TGG - tig0020520:53940-53959 Msa1382590:CDS 35.0%
GGACTGAGTTTAACATTAGT+CGG + tig0020520:54161-54180 None:intergenic 35.0%
AGAAGGAAACACTAACAATG+AGG + tig0020520:54367-54386 None:intergenic 35.0%
!!! CTGAATTTTTTGTCCTCCAT+AGG - tig0020520:54525-54544 Msa1382590:intron 35.0%
TTTCCACATCAAACTCTAGT+GGG + tig0020520:54698-54717 None:intergenic 35.0%
! TTTTCCACATCAAACTCTAG+TGG + tig0020520:54699-54718 None:intergenic 35.0%
!! ACAAAGGATAAGAAGCATCA+TGG + tig0020520:54753-54772 None:intergenic 35.0%
! AACTTCCTTTGAGTTTCCAA+AGG - tig0020520:54891-54910 Msa1382590:five_prime_UTR 35.0%
! ACTTCCTTTGAGTTTCCAAA+GGG - tig0020520:54892-54911 Msa1382590:five_prime_UTR 35.0%
CTAACCATGAAGAAAACACT+CGG + tig0020520:55106-55125 None:intergenic 35.0%
!!! AACACCTCATTTTTTTGCTC+CGG - tig0020520:55131-55150 Msa1382590:five_prime_UTR 35.0%
!!! ATGCTCATTTTTTCGCTCTA+GGG - tig0020520:55181-55200 Msa1382590:five_prime_UTR 35.0%
!!! ATGCTCATTTTTTCGCTCTA+GGG - tig0020520:55181-55200 Msa1382590:five_prime_UTR 35.0%
! AGGCAAGTGATAAAAGTGCA+AGG - tig0020520:50862-50881 Msa1382590:three_prime_UTR 40.0%
TTTGTTCTGTCCATGTGGTT+TGG - tig0020520:50961-50980 Msa1382590:three_prime_UTR 40.0%
AACAAAAAGGTGAGAGTGGA+TGG + tig0020520:51125-51144 Msa1382580:CDS 40.0%
TTTGTCCAAGACATTGCCTT+TGG - tig0020520:51144-51163 Msa1382590:three_prime_UTR 40.0%
ATACTCCAAAGGCAATGTCT+TGG + tig0020520:51152-51171 Msa1382580:CDS 40.0%
!! CATTGCCTTTGGAGTATTTG+AGG - tig0020520:51155-51174 Msa1382590:three_prime_UTR 40.0%
! AACTTGGAAAGCACAAAGAG+GGG + tig0020520:51198-51217 Msa1382580:CDS 40.0%
! GAACTTGGAAAGCACAAAGA+GGG + tig0020520:51199-51218 Msa1382580:CDS 40.0%
! AGAACTTGGAAAGCACAAAG+AGG + tig0020520:51200-51219 Msa1382580:CDS 40.0%
AGGAAATCTTGGAGAGAACT+TGG + tig0020520:51214-51233 Msa1382580:CDS 40.0%
AAAATGTTAACGAGTGCCTC+CGG - tig0020520:51412-51431 Msa1382590:three_prime_UTR 40.0%
! GAGTATTGTGAGAGAGATGA+AGG - tig0020520:51905-51924 Msa1382590:three_prime_UTR 40.0%
AGAGATGAAGGAGATGAAAG+AGG - tig0020520:51917-51936 Msa1382590:three_prime_UTR 40.0%
GAGATGAAGGAGATGAAAGA+GGG - tig0020520:51918-51937 Msa1382590:three_prime_UTR 40.0%
AAGGAGATGAAAGAGGGAAT+TGG - tig0020520:51924-51943 Msa1382590:three_prime_UTR 40.0%
GATATTGGTGAGGAACATTC+TGG + tig0020520:52014-52033 None:intergenic 40.0%
! TGCTGATAGTGATATTGGTG+AGG + tig0020520:52024-52043 None:intergenic 40.0%
!! GTTGGTGCTGATAGTGATAT+TGG + tig0020520:52029-52048 None:intergenic 40.0%
!! GATGATGATGATGGTGTTGT+TGG + tig0020520:52047-52066 None:intergenic 40.0%
! CTCTCTGATGATGATGATGA+TGG + tig0020520:52056-52075 None:intergenic 40.0%
CATCATCATCAGAGAGTCAA+TGG - tig0020520:52060-52079 Msa1382590:three_prime_UTR 40.0%
GTGCCTCAGTTTGTAGATTA+TGG - tig0020520:52174-52193 Msa1382590:three_prime_UTR 40.0%
GTTAAGACACTTGAACAGTG+TGG - tig0020520:52218-52237 Msa1382590:three_prime_UTR 40.0%
TTTCCCTATATCACTGAAGC+AGG - tig0020520:52250-52269 Msa1382590:three_prime_UTR 40.0%
CACTGAATTGGATTGTCTCT+TGG + tig0020520:52397-52416 None:intergenic 40.0%
TTATATCTTGGTCTAAGCCC+TGG - tig0020520:52454-52473 Msa1382590:CDS 40.0%
TGAGCACAACAATCTTACCA+GGG + tig0020520:52474-52493 None:intergenic 40.0%
ACCAAGTTCACGATCTTAGA+TGG - tig0020520:53061-53080 Msa1382590:intron 40.0%
! ACCATCTAAGATCGTGAACT+TGG + tig0020520:53065-53084 None:intergenic 40.0%
AAGTTGCAAGTTGCAGCAAA+AGG - tig0020520:53114-53133 Msa1382590:intron 40.0%
CTTCTTGGACTGAAGATATG+GGG + tig0020520:53264-53283 None:intergenic 40.0%
CCTTCTTGGACTGAAGATAT+GGG + tig0020520:53265-53284 None:intergenic 40.0%
TCCTTCTTGGACTGAAGATA+TGG + tig0020520:53266-53285 None:intergenic 40.0%
ATATCTTCAGTCCAAGAAGG+AGG - tig0020520:53265-53284 Msa1382590:intron 40.0%
TTCATGCTCATTGTGGCAAT+TGG + tig0020520:53317-53336 None:intergenic 40.0%
TTGCAGATTCATGCTCATTG+TGG + tig0020520:53324-53343 None:intergenic 40.0%
GAGCCAAATTCAACAACTTC+TGG + tig0020520:53358-53377 None:intergenic 40.0%
GGTGTTTGAATTTCAAGGGA+CGG - tig0020520:53455-53474 Msa1382590:intron 40.0%
GTGTTTGAATTTCAAGGGAC+GGG - tig0020520:53456-53475 Msa1382590:intron 40.0%
! GTTCTGCAGCTGAAACATTT+TGG + tig0020520:53534-53553 None:intergenic 40.0%
GAGAGGATATCGATAATCCA+TGG + tig0020520:53605-53624 None:intergenic 40.0%
AGGAGTTATCATTCACAAGC+TGG + tig0020520:53676-53695 None:intergenic 40.0%
CAGCTAGCATTACACAAACT+TGG + tig0020520:53783-53802 None:intergenic 40.0%
TGTTGTCAACACTTTCTTGG+TGG - tig0020520:53943-53962 Msa1382590:CDS 40.0%
AACACTTTCTTGGTGGAAAG+TGG - tig0020520:53950-53969 Msa1382590:CDS 40.0%
AACTGAACTTGCGACCATAA+CGG - tig0020520:54103-54122 Msa1382590:CDS 40.0%
ATAAAGACACTCACAATCGC+AGG + tig0020520:54182-54201 None:intergenic 40.0%
! ATGGTTTCTACAGGCTTTTG+TGG - tig0020520:54213-54232 Msa1382590:five_prime_UTR 40.0%
GAAGTGTAACTTGAACTCCA+GGG - tig0020520:54235-54254 Msa1382590:intron 40.0%
TCAATCAATGAAAGAGACCC+TGG + tig0020520:54255-54274 None:intergenic 40.0%
GAAGAAGACTCAATGGAACA+TGG + tig0020520:54345-54364 None:intergenic 40.0%
CAATGAGGAAGAAGACTCAA+TGG + tig0020520:54352-54371 None:intergenic 40.0%
!! AGCATTGATGTGACAACAGA+TGG + tig0020520:54390-54409 None:intergenic 40.0%
TGTCACATCAATGCTCTCTT+CGG - tig0020520:54395-54414 Msa1382590:intron 40.0%
AGGGTCAATCCACATTCATA+GGG + tig0020520:54436-54455 None:intergenic 40.0%
AAGGGTCAATCCACATTCAT+AGG + tig0020520:54437-54456 None:intergenic 40.0%
TTCAGTGTCATAACACTGAC+TGG + tig0020520:54510-54529 None:intergenic 40.0%
TTTCCCACTAGAGTTTGATG+TGG - tig0020520:54692-54711 Msa1382590:intron 40.0%
! GATGCTTTGGCATGTTGATT+CGG - tig0020520:54783-54802 Msa1382590:intron 40.0%
! CTTCCTTTGAGTTTCCAAAG+GGG - tig0020520:54893-54912 Msa1382590:five_prime_UTR 40.0%
AATAACACCAAAGTCCCCTT+TGG + tig0020520:54910-54929 None:intergenic 40.0%
GTGTGCTTAGAAAGATTCTC+TGG - tig0020520:54966-54985 Msa1382590:five_prime_UTR 40.0%
AAGATTCTCTGGTTGTGACA+CGG - tig0020520:54977-54996 Msa1382590:five_prime_UTR 40.0%
! ACACGGGGATGATTTTCAAT+GGG - tig0020520:54994-55013 Msa1382590:five_prime_UTR 40.0%
TTCAATGGGGACTTCATCTT+TGG - tig0020520:55008-55027 Msa1382590:five_prime_UTR 40.0%
!! ATCTTTGGTTCTGCTTCACT+TGG - tig0020520:55023-55042 Msa1382590:five_prime_UTR 40.0%
GAAGATGTCAATGTCTGATC+CGG + tig0020520:55051-55070 None:intergenic 40.0%
CTACCTCGCAAGAAGAAAAA+GGG + tig0020520:55073-55092 None:intergenic 40.0%
TCTACCTCGCAAGAAGAAAA+AGG + tig0020520:55074-55093 None:intergenic 40.0%
! TATGCCGAGTGTTTTCTTCA+TGG - tig0020520:55099-55118 Msa1382590:five_prime_UTR 40.0%
!!! ACACCTCATTTTTTTGCTCC+GGG - tig0020520:55132-55151 Msa1382590:five_prime_UTR 40.0%
!!! GATGCTCATTTTTTCGCTCT+AGG - tig0020520:55180-55199 Msa1382590:five_prime_UTR 40.0%
!!! GATGCTCATTTTTTCGCTCT+AGG - tig0020520:55180-55199 Msa1382590:five_prime_UTR 40.0%
AAAAAGGTGAGAGTGGATGG+AGG + tig0020520:51122-51141 Msa1382580:CDS 45.0%
AAATGTTAACGAGTGCCTCC+GGG - tig0020520:51413-51432 Msa1382590:three_prime_UTR 45.0%
TGCTTCCTTAACTAGTGCCA+CGG - tig0020520:51541-51560 Msa1382590:three_prime_UTR 45.0%
TGGAAGCTAGTGAAACCACT+TGG - tig0020520:52126-52145 Msa1382590:three_prime_UTR 45.0%
ATTGGATTGTCTCTTGGAGC+AGG + tig0020520:52391-52410 None:intergenic 45.0%
GTGAGCACAACAATCTTACC+AGG + tig0020520:52475-52494 None:intergenic 45.0%
GATGGAAGCGAAGAAACATG+TGG + tig0020520:53094-53113 None:intergenic 45.0%
TGCTGCAACTTGCAACTTGA+TGG + tig0020520:53112-53131 None:intergenic 45.0%
CTCAATGTTCTTCGTACGAG+AGG - tig0020520:53205-53224 Msa1382590:CDS 45.0%
CCCATATCTTCAGTCCAAGA+AGG - tig0020520:53262-53281 Msa1382590:intron 45.0%
TGCAGTTGTTCATGCCATCT+TGG + tig0020520:53430-53449 None:intergenic 45.0%
GGCATGAACAACTGCAATGT+TGG - tig0020520:53434-53453 Msa1382590:intron 45.0%
!!! GCAGCTGAAACATTTTGGCT+AGG + tig0020520:53529-53548 None:intergenic 45.0%
AAGGCTTGAAAAGCAGAGAG+AGG + tig0020520:53622-53641 None:intergenic 45.0%
GCTGCTCAAACAGATTGCAA+AGG + tig0020520:53641-53660 None:intergenic 45.0%
!! ACTCTCATTTTTCACCCTGC+AGG - tig0020520:53756-53775 Msa1382590:intron 45.0%
TATGGTCGCAAGTTCAGTTG+TGG + tig0020520:54102-54121 None:intergenic 45.0%
TGTGAGTGTCTTTATCGTCG+TGG - tig0020520:54187-54206 Msa1382590:exon 45.0%
GTCTTTATCGTCGTGGTTGA+TGG - tig0020520:54194-54213 Msa1382590:exon 45.0%
!! TCGTGGTTGATGGTTTCTAC+AGG - tig0020520:54204-54223 Msa1382590:five_prime_UTR 45.0%
! GGAAGTGTAACTTGAACTCC+AGG - tig0020520:54234-54253 Msa1382590:intron 45.0%
TCAATGGAACATGGTGAGGT+CGG + tig0020520:54336-54355 None:intergenic 45.0%
AGACTCAATGGAACATGGTG+AGG + tig0020520:54340-54359 None:intergenic 45.0%
GATGTGACAACAGATGGAGA+AGG + tig0020520:54384-54403 None:intergenic 45.0%
TCTTGTAGGCCCTATGAATG+TGG - tig0020520:54424-54443 Msa1382590:intron 45.0%
AGAGGAGCAACAATGCATGT+TGG + tig0020520:54480-54499 None:intergenic 45.0%
! ACACTGACTGGTGACAATAG+AGG + tig0020520:54498-54517 None:intergenic 45.0%
TTGTCCTCCATAGGTTGTGT+TGG - tig0020520:54534-54553 Msa1382590:intron 45.0%
AGTGCAACCAACACAACCTA+TGG + tig0020520:54544-54563 None:intergenic 45.0%
!! GGAAAGGCACCAATGCAAAA+AGG + tig0020520:54612-54631 None:intergenic 45.0%
AGCATCTTCTTCAGGCACAA+AGG + tig0020520:54769-54788 None:intergenic 45.0%
TGTGCCTGAAGAAGATGCTT+TGG - tig0020520:54770-54789 Msa1382590:intron 45.0%
! CATGCCAAAGCATCTTCTTC+AGG + tig0020520:54777-54796 None:intergenic 45.0%
!! CTTTGGCATGTTGATTCGGT+TGG - tig0020520:54787-54806 Msa1382590:intron 45.0%
! AGTCCCCTTTGGAAACTCAA+AGG + tig0020520:54899-54918 None:intergenic 45.0%
TGAGTTTCCAAAGGGGACTT+TGG - tig0020520:54900-54919 Msa1382590:five_prime_UTR 45.0%
AGATTCTCTGGTTGTGACAC+GGG - tig0020520:54978-54997 Msa1382590:five_prime_UTR 45.0%
! GACACGGGGATGATTTTCAA+TGG - tig0020520:54993-55012 Msa1382590:five_prime_UTR 45.0%
! CACGGGGATGATTTTCAATG+GGG - tig0020520:54995-55014 Msa1382590:five_prime_UTR 45.0%
! CTTCCCTTTTTCTTCTTGCG+AGG - tig0020520:55067-55086 Msa1382590:five_prime_UTR 45.0%
!!! CACCTCATTTTTTTGCTCCG+GGG - tig0020520:55133-55152 Msa1382590:five_prime_UTR 45.0%
GCATCATCCACCATATCCTT+CGG + tig0020520:55207-55226 None:intergenic 45.0%
GCATTATCCACCATCTCCTT+CGG + tig0020520:55249-55268 None:intergenic 45.0%
! TGGAAAGCACAAAGAGGGGA+GGG + tig0020520:51194-51213 Msa1382580:CDS 50.0%
! TTGGAAAGCACAAAGAGGGG+AGG + tig0020520:51195-51214 Msa1382580:CDS 50.0%
AATGTTAACGAGTGCCTCCG+GGG - tig0020520:51414-51433 Msa1382590:three_prime_UTR 50.0%
GCTTCCTTAACTAGTGCCAC+GGG - tig0020520:51542-51561 Msa1382590:three_prime_UTR 50.0%
CTTCCTTAACTAGTGCCACG+GGG - tig0020520:51543-51562 Msa1382590:three_prime_UTR 50.0%
AGGCACAAGCTACAAGTGCT+CGG + tig0020520:52160-52179 None:intergenic 50.0%
AGACACTTGAACAGTGTGGC+TGG - tig0020520:52222-52241 Msa1382590:three_prime_UTR 50.0%
GGAAGCGAAGAAACATGTGG+AGG + tig0020520:53091-53110 None:intergenic 50.0%
TTCTTCGTACGAGAGGTCCA+AGG - tig0020520:53212-53231 Msa1382590:CDS 50.0%
ATTGTACTCCGCATTCGCCT+TGG + tig0020520:53232-53251 None:intergenic 50.0%
TAGGAGCAGTTCCTCCTTCT+TGG + tig0020520:53279-53298 None:intergenic 50.0%
AGGAACTGCTCCTACTCCTA+TGG - tig0020520:53285-53304 Msa1382590:intron 50.0%
!! TTTCAAGGGACGGGTTACAG+TGG - tig0020520:53465-53484 Msa1382590:intron 50.0%
CGGGAAGGACATATTCACCA+TGG - tig0020520:53585-53604 Msa1382590:intron 50.0%
TACACAAACTTGGGCCTGCA+GGG + tig0020520:53773-53792 None:intergenic 50.0%
TTACACAAACTTGGGCCTGC+AGG + tig0020520:53774-53793 None:intergenic 50.0%
CAATGGAACATGGTGAGGTC+GGG + tig0020520:54335-54354 None:intergenic 50.0%
! CTCTTCGGACTCAGTCTTGT+AGG - tig0020520:54410-54429 Msa1382590:intron 50.0%
GCAACCAACACAACCTATGG+AGG + tig0020520:54541-54560 None:intergenic 50.0%
!! AGGTTGTGTTGGTTGCACTG+AGG - tig0020520:54545-54564 Msa1382590:intron 50.0%
ATCGAGCGAGGAAAGGGAAA+AGG + tig0020520:54646-54665 None:intergenic 50.0%
! TTTTCCCTTTCCTCGCTCGA+TGG - tig0020520:54645-54664 Msa1382590:intron 50.0%
TTTCCCTTTCCTCGCTCGAT+GGG - tig0020520:54646-54665 Msa1382590:intron 50.0%
AAGAATTGTCCCATCGAGCG+AGG + tig0020520:54658-54677 None:intergenic 50.0%
GATTCTCTGGTTGTGACACG+GGG - tig0020520:54979-54998 Msa1382590:five_prime_UTR 50.0%
GGTTCTGCTTCACTTGGAAC+CGG - tig0020520:55029-55048 Msa1382590:five_prime_UTR 50.0%
GACCCCGGAGCAAAAAAATG+AGG + tig0020520:55138-55157 None:intergenic 50.0%
!!! ATTTTTTTGCTCCGGGGTCC+AGG - tig0020520:55139-55158 Msa1382590:five_prime_UTR 50.0%
!!! TTTTTTCGCTCTAGGGCCGA+AGG - tig0020520:55188-55207 Msa1382590:five_prime_UTR 50.0%
!!! TTTTTTCGCTCTAGGGCCGA+AGG - tig0020520:55188-55207 Msa1382590:five_prime_UTR 50.0%
GTGAGAGTGGATGGAGGTTG+AGG + tig0020520:51116-51135 Msa1382580:CDS 55.0%
CACAAAGAGGGGAGGGAATG+TGG + tig0020520:51187-51206 Msa1382580:CDS 55.0%
AGCTTCCTTAACCAGTGCCC+CGG + tig0020520:51434-51453 Msa1382580:CDS 55.0%
TCTTGTTAACCAGTGCCCCG+TGG + tig0020520:51561-51580 Msa1382580:CDS 55.0%
TAGTTAGCAGGAGCAGCCCT+TGG + tig0020520:53160-53179 None:intergenic 55.0%
CGACCATAACGGCGAACTGT+TGG - tig0020520:54114-54133 Msa1382590:CDS 55.0%
CAGCCAACAGTTCGCCGTTA+TGG + tig0020520:54120-54139 None:intergenic 55.0%
CATAACGGCGAACTGTTGGC+TGG - tig0020520:54118-54137 Msa1382590:CDS 55.0%
TGTCCCATCGAGCGAGGAAA+GGG + tig0020520:54652-54671 None:intergenic 55.0%
TTGTCCCATCGAGCGAGGAA+AGG + tig0020520:54653-54672 None:intergenic 55.0%
AGCATCGTCCACCATCTCCT+TGG + tig0020520:55166-55185 None:intergenic 55.0%
! TCTAGGGCCGAAGGATATGG+TGG - tig0020520:55197-55216 Msa1382590:five_prime_UTR 55.0%
TTCCTTAACCAGTGCCCCGG+AGG + tig0020520:51431-51450 Msa1382580:CDS 60.0%
GTGCCCCGTGGCACTAGTTA+AGG + tig0020520:51549-51568 Msa1382580:CDS 60.0%
TAACTAGTGCCACGGGGCAC+TGG - tig0020520:51549-51568 Msa1382590:three_prime_UTR 60.0%
GCTACAAGTGCTCGGCGAGA+TGG + tig0020520:52152-52171 None:intergenic 60.0%
CGAGAGGTCCAAGGCGAATG+CGG - tig0020520:53221-53240 Msa1382590:CDS 60.0%
!! GGGACGGGTTACAGTGGCTT+CGG - tig0020520:53471-53490 Msa1382590:intron 60.0%
ATCTCCTTGGACCTGGACCC+CGG + tig0020520:55153-55172 None:intergenic 60.0%
GTCCAGGTCCAAGGAGATGG+TGG - tig0020520:55155-55174 Msa1382590:five_prime_UTR 60.0%
GTCCACCATCTCCTTGGACC+TGG + tig0020520:55160-55179 None:intergenic 60.0%
!! CGCTCTAGGGCCGAAGGATA+TGG - tig0020520:55194-55213 Msa1382590:five_prime_UTR 60.0%
! TCTAGGGCCGAAGGAGATGG+TGG - tig0020520:55239-55258 Msa1382590:five_prime_UTR 60.0%
!! TGCCTCCGGGGCACTGGTTA+AGG - tig0020520:51426-51445 Msa1382590:three_prime_UTR 65.0%
TGCTCGGCGAGATGGCCAAG+TGG + tig0020520:52144-52163 None:intergenic 65.0%
GGAACATGGTGAGGTCGGGC+AGG + tig0020520:54331-54350 None:intergenic 65.0%
GGGGTCCAGGTCCAAGGAGA+TGG - tig0020520:55152-55171 Msa1382590:five_prime_UTR 65.0%
!! CGCTCTAGGGCCGAAGGAGA+TGG - tig0020520:55236-55255 Msa1382590:five_prime_UTR 65.0%
AACGAGTGCCTCCGGGGCAC+TGG - tig0020520:51420-51439 Msa1382590:three_prime_UTR 70.0%
TGCTCCGGGGTCCAGGTCCA+AGG - tig0020520:55146-55165 Msa1382590:five_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
tig0020520 gene 50812 55284 50812 ID=Msa1382590;Name=Msa1382590
tig0020520 mRNA 50812 55284 50812 ID=Msa1382590-mRNA-1;Parent=Msa1382590;Name=Msa1382590-mRNA-1;_AED=0.09;_eAED=0.09;_QI=481|1|1|1|0.75|0.6|5|1597|413
tig0020520 exon 50812 53051 50812 ID=Msa1382590-mRNA-1:exon:8216;Parent=Msa1382590-mRNA-1
tig0020520 exon 53136 53268 53136 ID=Msa1382590-mRNA-1:exon:8215;Parent=Msa1382590-mRNA-1
tig0020520 exon 53621 53708 53621 ID=Msa1382590-mRNA-1:exon:8214;Parent=Msa1382590-mRNA-1
tig0020520 exon 53825 54237 53825 ID=Msa1382590-mRNA-1:exon:8213;Parent=Msa1382590-mRNA-1
tig0020520 exon 54839 55284 54839 ID=Msa1382590-mRNA-1:exon:8212;Parent=Msa1382590-mRNA-1
tig0020520 five_prime_UTR 54839 55284 54839 ID=Msa1382590-mRNA-1:five_prime_utr;Parent=Msa1382590-mRNA-1
tig0020520 five_prime_UTR 54203 54237 54203 ID=Msa1382590-mRNA-1:five_prime_utr;Parent=Msa1382590-mRNA-1
tig0020520 CDS 53825 54202 53825 ID=Msa1382590-mRNA-1:cds;Parent=Msa1382590-mRNA-1
tig0020520 CDS 53621 53708 53621 ID=Msa1382590-mRNA-1:cds;Parent=Msa1382590-mRNA-1
tig0020520 CDS 53136 53268 53136 ID=Msa1382590-mRNA-1:cds;Parent=Msa1382590-mRNA-1
tig0020520 CDS 52409 53051 52409 ID=Msa1382590-mRNA-1:cds;Parent=Msa1382590-mRNA-1
tig0020520 three_prime_UTR 50812 52408 50812 ID=Msa1382590-mRNA-1:three_prime_utr;Parent=Msa1382590-mRNA-1
Gene Sequence

>Msa1382590

ATGCCTTTCAAGAGTATTGTGAGAGAGATGAAGGAGATGAAAGAGGGAATTGGCAACATGTATAGAAGAGGTGTAGAAACTAAGCATATGCATAGACATGGAAAATCTCACATAGCACCAGAATGTTCCTCACCAATATCACTATCAGCACCAACAACACCATCATCATCATCATCAGAGAGTCAATGGGCTAATTTACCTCCTGAGTTACTATTAGACATAATTCAAAGGGTGGAAGCTAGTGAAACCACTTGGCCATCTCGCCGAGCACTTGTAGCTTGTGCCTCAGTTTGTAGATTATGGAGAGACATAACAAAGGGTGTTGTTAAGACACTTGAACAGTGTGGCTGGATCACTTTCCCTATATCACTGAAGCAGCCTGCTCCAAGAGACAATCCAATTCAGTGTTTTATTAAGAGAGAAAGGGCTACTTCCACATATAGTTTATATCTTGGTCTAAGCCCTGCTCTTTCTGGTGATATGAGCAAACTTCTCTTGGCAGCAAAGAAGATTAGAAGAGCAACATGCACAGAATTTTTAATATCATTGGTTTCTGATGATTTCTCTAGAGCTAACAATACTTACATTGGAAAGTTAAGGTCTAATTTTCTCGGCACCAAGTTCACGATCTTAGATGGTCATCCTCCACATGTTTCTTCGCTTCCATCAAGTTGCAAGTTGCAGCAAAAGGTCCATATGAAGCAAGTACTTCCAAGGGCTGCTCCTGCTAACTATAAAGTAGCAACTGTATCTTATGAACTCAATGTTCTTCGTACGAGAGGTCCAAGGCGAATGCGGAGTACAATGCACTTAATCCCCATATCTTCAGTCCAAGAAGGAGGAACTGCTCCTACTCCTATGGAATTTACCAATTGCCACAATGAGCATGAATCTGCAAAAGAAAAGAAGCCAGAAGTTGTTGAATTTGGCTCAACTTGCACTGATTTTGCAAGAGAGCCTCTGATTTTGAAAAACAAAGCACCAAGATGGCATGAACAACTGCAATGTTGGTGTTTGAATTTCAAGGGACGGGTTACAGTGGCTTCGGTGAAGAATTTTCAGCTTGTAGCAGTTGCAGAACCTAGCCAAAATGTTTCAGCTGCAGAACAAGAAAAAGTTATACTACAATTTGGAAAAATCGGGAAGGACATATTCACCATGGATTATCGATATCCTCTCTCTGCTTTTCAAGCCTTTGCAATCTGTTTGAGCAGCTTTGACACAAAACCAGCTTGTGAATGA
Protein sequence

>Msa1382590

MPFKSIVREMKEMKEGIGNMYRRGVETKHMHRHGKSHIAPECSSPISLSAPTTPSSSSSESQWANLPPELLLDIIQRVEASETTWPSRRALVACASVCRLWRDITKGVVKTLEQCGWITFPISLKQPAPRDNPIQCFIKRERATSTYSLYLGLSPALSGDMSKLLLAAKKIRRATCTEFLISLVSDDFSRANNTYIGKLRSNFLGTKFTILDGHPPHVSSLPSSCKLQQKVHMKQVLPRAAPANYKVATVSYELNVLRTRGPRRMRSTMHLIPISSVQEGGTAPTPMEFTNCHNEHESAKEKKPEVVEFGSTCTDFAREPLILKNKAPRWHEQLQCWCLNFKGRVTVASVKNFQLVAVAEPSQNVSAAEQEKVILQFGKIGKDIFTMDYRYPLSAFQAFAICLSSFDTKPACE*