Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1391980 | AGC78955.1 | 97.959 | 98 | 2 | 0 | 4 | 101 | 7 | 104 | 1.38e-64 | 200 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1391980 | R4ITY9 | 97.959 | 98 | 2 | 0 | 4 | 101 | 7 | 104 | 6.57e-65 | 200 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0069500 | Msa1391980 | 0.801648 | 8.153328e-49 | -8.615850e-47 |
| Msa1391970 | Msa1391980 | 0.840055 | 1.094937e-57 | -8.615850e-47 |
| Msa1391980 | Msa1392030 | 0.810175 | 1.313681e-50 | -8.615850e-47 |
| Msa1391980 | Msa1392160 | 0.808552 | 2.928056e-50 | -8.615850e-47 |
| Msa1391980 | Msa1453860 | 0.916732 | 1.249323e-85 | -8.615850e-47 |
| Msa1391980 | Msa1453870 | 0.815940 | 7.146989e-52 | -8.615850e-47 |
| Msa0983300 | Msa1391980 | 0.820728 | 5.883414e-53 | -8.615850e-47 |
| Msa0983350 | Msa1391980 | 0.842618 | 2.316681e-58 | -8.615850e-47 |
| Msa0920010 | Msa1391980 | 0.852318 | 4.993241e-61 | -8.615850e-47 |
| Msa0920060 | Msa1391980 | 0.815794 | 7.700879e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCGAACGCGTTCGAGTTAT+TGG | 0.162538 | tig0021468:+41810 | None:intergenic |
| TTGGCTGAGCCGTATCTTGC+TGG | 0.274191 | tig0021468:-41940 | Msa1391980:CDS |
| ATACGGACTCCAAGTTCTTA+TGG | 0.378715 | tig0021468:-41742 | Msa1391980:CDS |
| CGCGCTCTGCCATAAGAACT+TGG | 0.425834 | tig0021468:+41733 | None:intergenic |
| GCCAATAACTCGAACGCGTT+CGG | 0.430894 | tig0021468:-41811 | Msa1391980:CDS |
| GGTGGGACCGAGAGCAAGAA+AGG | 0.489559 | tig0021468:-41918 | Msa1391980:CDS |
| TGGCTGAGCCGTATCTTGCT+GGG | 0.491654 | tig0021468:-41939 | Msa1391980:CDS |
| GACCGAGAGCAAGAAAGGAC+GGG | 0.491917 | tig0021468:-41913 | Msa1391980:CDS |
| GAGCGACTCATGAATGCTGC+CGG | 0.495040 | tig0021468:-41843 | Msa1391980:CDS |
| GGACCGAGAGCAAGAAAGGA+CGG | 0.512094 | tig0021468:-41914 | Msa1391980:CDS |
| ACCTTACCTTCACCATGATA+CGG | 0.518013 | tig0021468:-41759 | Msa1391980:CDS |
| ACTCATGAATGCTGCCGGGT+TGG | 0.526859 | tig0021468:-41838 | Msa1391980:CDS |
| TCCGTATCATGGTGAAGGTA+AGG | 0.538798 | tig0021468:+41758 | None:intergenic |
| TGAGCCGTATCTTGCTGGGT+GGG | 0.552172 | tig0021468:-41935 | Msa1391980:CDS |
| ACCGAGAGCAAGAAAGGACG+GGG | 0.558920 | tig0021468:-41912 | Msa1391980:CDS |
| CGGTCCCACCCAGCAAGATA+CGG | 0.559998 | tig0021468:+41931 | None:intergenic |
| AGAACTTGGAGTCCGTATCA+TGG | 0.567784 | tig0021468:+41747 | None:intergenic |
| AGCGACTCATGAATGCTGCC+GGG | 0.590061 | tig0021468:-41842 | Msa1391980:CDS |
| AGTTATTGGCTCGTCCAACC+CGG | 0.599786 | tig0021468:+41824 | None:intergenic |
| CCGAGAGCAAGAAAGGACGG+GGG | 0.605735 | tig0021468:-41911 | Msa1391980:CDS |
| TCAACTGTCCCTCGGAGACA+CGG | 0.609693 | tig0021468:+41868 | None:intergenic |
| TGGAGTCCGTATCATGGTGA+AGG | 0.610039 | tig0021468:+41753 | None:intergenic |
| AGTTCTTATGGCAGAGCGCG+AGG | 0.610368 | tig0021468:-41730 | Msa1391980:CDS |
| GACACGGTCGAGAACATGCA+TGG | 0.621948 | tig0021468:+41884 | None:intergenic |
| CGAGAGCAAGAAAGGACGGG+GGG | 0.629548 | tig0021468:-41910 | Msa1391980:CDS |
| TGTTCTCGACCGTGTCTCCG+AGG | 0.631514 | tig0021468:-41877 | Msa1391980:CDS |
| CTGAGCCGTATCTTGCTGGG+TGG | 0.641566 | tig0021468:-41936 | Msa1391980:CDS |
| GTTCTCGACCGTGTCTCCGA+GGG | 0.655495 | tig0021468:-41876 | Msa1391980:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTGACTTTACTAAAAAAATA+AGG | - | tig0021468:41851-41870 | Msa1391980:CDS | 15.0% |
| !! | ACAGAAATGGATAAAAAAAA+GGG | + | tig0021468:41807-41826 | None:intergenic | 20.0% |
| ! | AGAAATGGATAAAAAAAAGG+GGG | + | tig0021468:41805-41824 | None:intergenic | 25.0% |
| ! | CAGAAATGGATAAAAAAAAG+GGG | + | tig0021468:41806-41825 | None:intergenic | 25.0% |
| ! | GACAGAAATGGATAAAAAAA+AGG | + | tig0021468:41808-41827 | None:intergenic | 25.0% |
| !! | ATTTCTGTCTTTAGTCTTTA+GGG | - | tig0021468:41819-41838 | Msa1391980:CDS | 25.0% |
| !!! | TTTTTAGTAAAGTCAAGCTT+TGG | + | tig0021468:41847-41866 | None:intergenic | 25.0% |
| GAAATGGATAAAAAAAAGGG+GGG | + | tig0021468:41804-41823 | None:intergenic | 30.0% | |
| CTAAAGACTAAAGACAGAAA+TGG | + | tig0021468:41820-41839 | None:intergenic | 30.0% | |
| ! | CATTTCTGTCTTTAGTCTTT+AGG | - | tig0021468:41818-41837 | Msa1391980:CDS | 30.0% |
| ACCTTACCTTCACCATGATA+CGG | - | tig0021468:42079-42098 | Msa1391980:intron | 40.0% | |
| ATACGGACTCCAAGTTCTTA+TGG | - | tig0021468:42096-42115 | Msa1391980:intron | 40.0% | |
| ACATTGTAGGTGCTTGCGAT+GGG | - | tig0021468:41726-41745 | Msa1391980:CDS | 45.0% | |
| ATACCACCCAAACCAACTGA+GGG | + | tig0021468:41765-41784 | None:intergenic | 45.0% | |
| TATACCACCCAAACCAACTG+AGG | + | tig0021468:41766-41785 | None:intergenic | 45.0% | |
| TAAAAAAATAAGGCCCGCGC+AGG | - | tig0021468:41861-41880 | Msa1391980:CDS | 45.0% | |
| AAAAAAATAAGGCCCGCGCA+GGG | - | tig0021468:41862-41881 | Msa1391980:CDS | 45.0% | |
| TCCGTATCATGGTGAAGGTA+AGG | + | tig0021468:42083-42102 | None:intergenic | 45.0% | |
| AGAACTTGGAGTCCGTATCA+TGG | + | tig0021468:42094-42113 | None:intergenic | 45.0% | |
| GACATTGTAGGTGCTTGCGA+TGG | - | tig0021468:41725-41744 | Msa1391980:CDS | 50.0% | |
| CATTGTAGGTGCTTGCGATG+GGG | - | tig0021468:41727-41746 | Msa1391980:CDS | 50.0% | |
| ! | CGACTTCCCTCAGTTGGTTT+GGG | - | tig0021468:41756-41775 | Msa1391980:CDS | 50.0% |
| AAAAGGGGGGACTTCTGCAA+CGG | + | tig0021468:41791-41810 | None:intergenic | 50.0% | |
| AAAAAATAAGGCCCGCGCAG+GGG | - | tig0021468:41863-41882 | Msa1391980:CDS | 50.0% | |
| !! | AGTTATTGGCTCGTCCAACC+CGG | + | tig0021468:42017-42036 | None:intergenic | 50.0% |
| GCCAATAACTCGAACGCGTT+CGG | - | tig0021468:42027-42046 | Msa1391980:intron | 50.0% | |
| ! | ACCGAACGCGTTCGAGTTAT+TGG | + | tig0021468:42031-42050 | None:intergenic | 50.0% |
| TGGAGTCCGTATCATGGTGA+AGG | + | tig0021468:42088-42107 | None:intergenic | 50.0% | |
| GCGACTTCCCTCAGTTGGTT+TGG | - | tig0021468:41755-41774 | Msa1391980:CDS | 55.0% | |
| !! | CTTCCCTCAGTTGGTTTGGG+TGG | - | tig0021468:41759-41778 | Msa1391980:CDS | 55.0% |
| AAAGGGGGGACTTCTGCAAC+GGG | + | tig0021468:41790-41809 | None:intergenic | 55.0% | |
| TTGGCTGAGCCGTATCTTGC+TGG | - | tig0021468:41898-41917 | Msa1391980:CDS | 55.0% | |
| TGGCTGAGCCGTATCTTGCT+GGG | - | tig0021468:41899-41918 | Msa1391980:CDS | 55.0% | |
| TGAGCCGTATCTTGCTGGGT+GGG | - | tig0021468:41903-41922 | Msa1391980:CDS | 55.0% | |
| GGACCGAGAGCAAGAAAGGA+CGG | - | tig0021468:41924-41943 | Msa1391980:CDS | 55.0% | |
| GACCGAGAGCAAGAAAGGAC+GGG | - | tig0021468:41925-41944 | Msa1391980:CDS | 55.0% | |
| ACCGAGAGCAAGAAAGGACG+GGG | - | tig0021468:41926-41945 | Msa1391980:CDS | 55.0% | |
| GACACGGTCGAGAACATGCA+TGG | + | tig0021468:41957-41976 | None:intergenic | 55.0% | |
| TCAACTGTCCCTCGGAGACA+CGG | + | tig0021468:41973-41992 | None:intergenic | 55.0% | |
| TCGCTCGTTCAACTGTCCCT+CGG | + | tig0021468:41981-42000 | None:intergenic | 55.0% | |
| GAGCGACTCATGAATGCTGC+CGG | - | tig0021468:41995-42014 | Msa1391980:CDS | 55.0% | |
| AGCGACTCATGAATGCTGCC+GGG | - | tig0021468:41996-42015 | Msa1391980:CDS | 55.0% | |
| ACTCATGAATGCTGCCGGGT+TGG | - | tig0021468:42000-42019 | Msa1391980:CDS | 55.0% | |
| CGCGCTCTGCCATAAGAACT+TGG | + | tig0021468:42108-42127 | None:intergenic | 55.0% | |
| AGTTCTTATGGCAGAGCGCG+AGG | - | tig0021468:42108-42127 | Msa1391980:intron | 55.0% | |
| TGTAGGTGCTTGCGATGGGG+CGG | - | tig0021468:41730-41749 | Msa1391980:CDS | 60.0% | |
| CGGATGCGACTTCCCTCAGT+TGG | - | tig0021468:41750-41769 | Msa1391980:CDS | 60.0% | |
| !!! | GCAGGGGCGCTCACGTTTTT+TGG | - | tig0021468:41879-41898 | Msa1391980:CDS | 60.0% |
| CTGAGCCGTATCTTGCTGGG+TGG | - | tig0021468:41902-41921 | Msa1391980:CDS | 60.0% | |
| CGGTCCCACCCAGCAAGATA+CGG | + | tig0021468:41910-41929 | None:intergenic | 60.0% | |
| GGTGGGACCGAGAGCAAGAA+AGG | - | tig0021468:41920-41939 | Msa1391980:CDS | 60.0% | |
| CCCCCGTCCTTTCTTGCTCT+CGG | + | tig0021468:41930-41949 | None:intergenic | 60.0% | |
| CCGAGAGCAAGAAAGGACGG+GGG | - | tig0021468:41927-41946 | Msa1391980:CDS | 60.0% | |
| CGAGAGCAAGAAAGGACGGG+GGG | - | tig0021468:41928-41947 | Msa1391980:CDS | 60.0% | |
| TGTTCTCGACCGTGTCTCCG+AGG | - | tig0021468:41961-41980 | Msa1391980:CDS | 60.0% | |
| GTTCTCGACCGTGTCTCCGA+GGG | - | tig0021468:41962-41981 | Msa1391980:CDS | 60.0% | |
| AAAACGTGAGCGCCCCTGCG+CGG | + | tig0021468:41878-41897 | None:intergenic | 65.0% | |
| AAACGTGAGCGCCCCTGCGC+GGG | + | tig0021468:41877-41896 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0021468 | gene | 41723 | 42137 | 41723 | ID=Msa1391980;Name=Msa1391980 |
| tig0021468 | mRNA | 41723 | 42137 | 41723 | ID=Msa1391980-mRNA-1;Parent=Msa1391980;Name=Msa1391980-mRNA-1;_AED=0.47;_eAED=0.49;_QI=0|0|0|1|1|1|2|0|101 |
| tig0021468 | exon | 42130 | 42137 | 42130 | ID=Msa1391980-mRNA-1:exon:12981;Parent=Msa1391980-mRNA-1 |
| tig0021468 | exon | 41723 | 42020 | 41723 | ID=Msa1391980-mRNA-1:exon:12980;Parent=Msa1391980-mRNA-1 |
| tig0021468 | CDS | 42130 | 42137 | 42130 | ID=Msa1391980-mRNA-1:cds;Parent=Msa1391980-mRNA-1 |
| tig0021468 | CDS | 41723 | 42020 | 41723 | ID=Msa1391980-mRNA-1:cds;Parent=Msa1391980-mRNA-1 |
| Gene Sequence |
| Protein sequence |
