AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1453860


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1453860 MtrunA17_MTg0491601 90.625 96 4 1 1 96 37 127 3.47e-54 169
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1453860 ATMG00580.1 87.640 89 11 0 1 89 374 462 6.87e-44 152

Find 61 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATATAATCGTGTGGTTTC+TGG 0.115346 tig0034911:+23148 Msa1453860:CDS
CATTTCTGTCTTTAGTCTTT+AGG 0.139002 tig0034911:+23400 Msa1453860:CDS
ACCGAACGCGTTCGAGTTAT+TGG 0.162538 tig0034911:-23610 None:intergenic
ATTTCTGTCTTTAGTCTTTA+GGG 0.214411 tig0034911:+23401 Msa1453860:CDS
CATTTGGATCGGAGAATTTA+TGG 0.225223 tig0034911:-23192 None:intergenic
TTGGCTGAGCCGTATCTTGC+TGG 0.274191 tig0034911:+23480 Msa1453860:CDS
TGGCTACTAAGCTATTTCTT+TGG 0.294828 tig0034911:-23078 None:intergenic
CCTGGTACTAGCAGCTTTAT+CGG 0.335139 tig0034911:+23028 Msa1453860:CDS
ATGGAAACTTCTCTGCCATT+TGG 0.362258 tig0034911:-23208 None:intergenic
ATACGGACTCCAAGTTCTTA+TGG 0.378715 tig0034911:+23678 Msa1453860:CDS
CTGGTACTAGCAGCTTTATC+GGG 0.404966 tig0034911:+23029 Msa1453860:CDS
CTAAAGACTAAAGACAGAAA+TGG 0.416280 tig0034911:-23399 None:intergenic
CGCGCTCTGCCATAAGAACT+TGG 0.425834 tig0034911:-23687 None:intergenic
GCCAATAACTCGAACGCGTT+CGG 0.430894 tig0034911:+23609 Msa1453860:CDS
AGTACCAGGTGAACTCATAT+TGG 0.455273 tig0034911:-23014 None:intergenic
GGAGAATTTATGGAGGAAAT+CGG 0.458292 tig0034911:-23182 None:intergenic
TAGGCGCGGCGTATTCCCTT+TGG 0.468628 tig0034911:+23125 Msa1453860:CDS
CATTTCCTTCTCTTCCACTT+TGG 0.469790 tig0034911:+22991 Msa1453860:CDS
AAGGAAATGGTAGAGAGATT+CGG 0.474409 tig0034911:-22977 None:intergenic
CCGATAAAGCTGCTAGTACC+AGG 0.489275 tig0034911:-23028 None:intergenic
GTAGCCACATTAGCAGCGCT+TGG 0.489321 tig0034911:+23094 Msa1453860:CDS
GGTGGGACCGAGAGCAAGAA+AGG 0.489559 tig0034911:+23502 Msa1453860:CDS
CATAAATTCTCCGATCCAAA+TGG 0.490582 tig0034911:+23193 Msa1453860:CDS
TGGCTGAGCCGTATCTTGCT+GGG 0.491654 tig0034911:+23481 Msa1453860:CDS
GACCGAGAGCAAGAAAGGAC+GGG 0.491917 tig0034911:+23507 Msa1453860:CDS
TTGGAAAGCTCCTACTGAGA+TGG 0.492362 tig0034911:-23059 None:intergenic
GAGCGACTCATGAATGCTGC+CGG 0.495040 tig0034911:+23577 Msa1453860:CDS
AACTTCTCTGCCATTTGGAT+CGG 0.499588 tig0034911:-23203 None:intergenic
TAGCCACATTAGCAGCGCTT+GGG 0.509701 tig0034911:+23095 Msa1453860:CDS
CATCCCAAGCGCTGCTAATG+TGG 0.509853 tig0034911:-23098 None:intergenic
GGACCGAGAGCAAGAAAGGA+CGG 0.512094 tig0034911:+23506 Msa1453860:CDS
ACCTTACCTTCACCATGATA+CGG 0.518013 tig0034911:+23661 Msa1453860:CDS
ACTCATGAATGCTGCCGGGT+TGG 0.526859 tig0034911:+23582 Msa1453860:CDS
TGGAAAGCTCCTACTGAGAT+GGG 0.532780 tig0034911:-23058 None:intergenic
TCCGTATCATGGTGAAGGTA+AGG 0.538798 tig0034911:-23662 None:intergenic
CACACGATTATATAGCCAAA+GGG 0.551184 tig0034911:-23140 None:intergenic
TGAGCCGTATCTTGCTGGGT+GGG 0.552172 tig0034911:+23485 Msa1453860:CDS
ACCGAGAGCAAGAAAGGACG+GGG 0.558920 tig0034911:+23508 Msa1453860:CDS
CGGTCCCACCCAGCAAGATA+CGG 0.559998 tig0034911:-23489 None:intergenic
ATTGGCCAAAGTGGAAGAGA+AGG 0.560732 tig0034911:-22996 None:intergenic
AGAACTTGGAGTCCGTATCA+TGG 0.567784 tig0034911:-23673 None:intergenic
CAAAGTGGAAGAGAAGGAAA+TGG 0.570201 tig0034911:-22990 None:intergenic
TGAACTCATATTGGCCAAAG+TGG 0.575500 tig0034911:-23005 None:intergenic
CCTTTGGCTATATAATCGTG+TGG 0.579601 tig0034911:+23141 Msa1453860:CDS
AGCGACTCATGAATGCTGCC+GGG 0.590061 tig0034911:+23578 Msa1453860:CDS
AGTTATTGGCTCGTCCAACC+CGG 0.599786 tig0034911:-23596 None:intergenic
CCGAGAGCAAGAAAGGACGG+GGG 0.605735 tig0034911:+23509 Msa1453860:CDS
TTGGCCAATATGAGTTCACC+TGG 0.605951 tig0034911:+23010 Msa1453860:CDS
GGGGAATTTCCCATCTCAGT+AGG 0.607104 tig0034911:+23049 Msa1453860:CDS
TCAACTGTCCCTCGGAGACA+CGG 0.609693 tig0034911:-23552 None:intergenic
TGGAGTCCGTATCATGGTGA+AGG 0.610039 tig0034911:-23667 None:intergenic
AGTTCTTATGGCAGAGCGCG+AGG 0.610368 tig0034911:+23690 Msa1453860:CDS
GACACGGTCGAGAACATGCA+TGG 0.621948 tig0034911:-23536 None:intergenic
CCACACGATTATATAGCCAA+AGG 0.624841 tig0034911:-23141 None:intergenic
CGAGAGCAAGAAAGGACGGG+GGG 0.629548 tig0034911:+23510 Msa1453860:CDS
TGTTCTCGACCGTGTCTCCG+AGG 0.631514 tig0034911:+23543 Msa1453860:CDS
CTGAGCCGTATCTTGCTGGG+TGG 0.641566 tig0034911:+23484 Msa1453860:CDS
GTTCTCGACCGTGTCTCCGA+GGG 0.655495 tig0034911:+23544 Msa1453860:CDS
TGGTACTAGCAGCTTTATCG+GGG 0.656393 tig0034911:+23030 Msa1453860:CDS
AATGGTAGAGAGATTCGGCA+TGG 0.678484 tig0034911:-22972 None:intergenic
TTGGATCGGAGAATTTATGG+AGG 0.680463 tig0034911:-23189 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGACTTTACTAAAAAAATA+AGG + tig0034911:23433-23452 Msa1453860:CDS 15.0%
!!! AACAAGAAAAGGTATAAATA+TGG - tig0034911:23230-23249 None:intergenic 20.0%
!!! ATATTTATACCTTTTCTTGT+TGG + tig0034911:23229-23248 Msa1453860:intron 20.0%
!! ACAGAAATGGATAAAAAAAA+GGG - tig0034911:23389-23408 None:intergenic 20.0%
!!! TTTTTTCTTTGGTAGTTCAA+CGG - tig0034911:23267-23286 None:intergenic 25.0%
! TTGAACTACCAAAGAAAAAA+GGG + tig0034911:23267-23286 Msa1453860:intron 25.0%
! AGAAATGGATAAAAAAAAGG+GGG - tig0034911:23387-23406 None:intergenic 25.0%
! CAGAAATGGATAAAAAAAAG+GGG - tig0034911:23388-23407 None:intergenic 25.0%
! GACAGAAATGGATAAAAAAA+AGG - tig0034911:23390-23409 None:intergenic 25.0%
!! ATTTCTGTCTTTAGTCTTTA+GGG + tig0034911:23401-23420 Msa1453860:CDS 25.0%
!!! TTTTTAGTAAAGTCAAGCTT+TGG - tig0034911:23429-23448 None:intergenic 25.0%
! TTTATACCTTTTCTTGTTGG+AGG + tig0034911:23232-23251 Msa1453860:intron 30.0%
! TTATACCTTTTCTTGTTGGA+GGG + tig0034911:23233-23252 Msa1453860:intron 30.0%
GTTGAACTACCAAAGAAAAA+AGG + tig0034911:23266-23285 Msa1453860:intron 30.0%
!!! TTGGTTTACCCTTTTTTCTT+TGG - tig0034911:23278-23297 None:intergenic 30.0%
GAAATGGATAAAAAAAAGGG+GGG - tig0034911:23386-23405 None:intergenic 30.0%
CTAAAGACTAAAGACAGAAA+TGG - tig0034911:23402-23421 None:intergenic 30.0%
! CATTTCTGTCTTTAGTCTTT+AGG + tig0034911:23400-23419 Msa1453860:CDS 30.0%
AAGGAAATGGTAGAGAGATT+CGG - tig0034911:22980-22999 None:intergenic 35.0%
!! TGGCTACTAAGCTATTTCTT+TGG - tig0034911:23081-23100 None:intergenic 35.0%
CACACGATTATATAGCCAAA+GGG - tig0034911:23143-23162 None:intergenic 35.0%
CTATATAATCGTGTGGTTTC+TGG + tig0034911:23148-23167 Msa1453860:CDS 35.0%
GGAGAATTTATGGAGGAAAT+CGG - tig0034911:23185-23204 None:intergenic 35.0%
CATTTGGATCGGAGAATTTA+TGG - tig0034911:23195-23214 None:intergenic 35.0%
CATAAATTCTCCGATCCAAA+TGG + tig0034911:23193-23212 Msa1453860:CDS 35.0%
! TATACCTTTTCTTGTTGGAG+GGG + tig0034911:23234-23253 Msa1453860:intron 35.0%
CTACAATGTCATGATCACAT+TGG - tig0034911:23297-23316 None:intergenic 35.0%
CAATGTGATCATGACATTGT+AGG + tig0034911:23295-23314 Msa1453860:intron 35.0%
CAAAGTGGAAGAGAAGGAAA+TGG - tig0034911:22993-23012 None:intergenic 40.0%
CATTTCCTTCTCTTCCACTT+TGG + tig0034911:22991-23010 Msa1453860:CDS 40.0%
TGAACTCATATTGGCCAAAG+TGG - tig0034911:23008-23027 None:intergenic 40.0%
AGTACCAGGTGAACTCATAT+TGG - tig0034911:23017-23036 None:intergenic 40.0%
CCACACGATTATATAGCCAA+AGG - tig0034911:23144-23163 None:intergenic 40.0%
CCTTTGGCTATATAATCGTG+TGG + tig0034911:23141-23160 Msa1453860:CDS 40.0%
TTGGATCGGAGAATTTATGG+AGG - tig0034911:23192-23211 None:intergenic 40.0%
! AACTTCTCTGCCATTTGGAT+CGG - tig0034911:23206-23225 None:intergenic 40.0%
ATGGAAACTTCTCTGCCATT+TGG - tig0034911:23211-23230 None:intergenic 40.0%
! TTCTTTGGTAGTTCAACGGA+CGG - tig0034911:23263-23282 None:intergenic 40.0%
ACCTTACCTTCACCATGATA+CGG + tig0034911:23661-23680 Msa1453860:CDS 40.0%
ATACGGACTCCAAGTTCTTA+TGG + tig0034911:23678-23697 Msa1453860:CDS 40.0%
ATTGGCCAAAGTGGAAGAGA+AGG - tig0034911:22999-23018 None:intergenic 45.0%
TTGGCCAATATGAGTTCACC+TGG + tig0034911:23010-23029 Msa1453860:CDS 45.0%
! CCTGGTACTAGCAGCTTTAT+CGG + tig0034911:23028-23047 Msa1453860:CDS 45.0%
! CTGGTACTAGCAGCTTTATC+GGG + tig0034911:23029-23048 Msa1453860:CDS 45.0%
! TGGTACTAGCAGCTTTATCG+GGG + tig0034911:23030-23049 Msa1453860:CDS 45.0%
TGGAAAGCTCCTACTGAGAT+GGG - tig0034911:23061-23080 None:intergenic 45.0%
TTGGAAAGCTCCTACTGAGA+TGG - tig0034911:23062-23081 None:intergenic 45.0%
ACATTGTAGGTGCTTGCGAT+GGG + tig0034911:23308-23327 Msa1453860:intron 45.0%
ATACCACCCAAACCAACTGA+GGG - tig0034911:23347-23366 None:intergenic 45.0%
TATACCACCCAAACCAACTG+AGG - tig0034911:23348-23367 None:intergenic 45.0%
TAAAAAAATAAGGCCCGCGC+AGG + tig0034911:23443-23462 Msa1453860:CDS 45.0%
AAAAAAATAAGGCCCGCGCA+GGG + tig0034911:23444-23463 Msa1453860:CDS 45.0%
TCCGTATCATGGTGAAGGTA+AGG - tig0034911:23665-23684 None:intergenic 45.0%
AGAACTTGGAGTCCGTATCA+TGG - tig0034911:23676-23695 None:intergenic 45.0%
CCGATAAAGCTGCTAGTACC+AGG - tig0034911:23031-23050 None:intergenic 50.0%
GGGGAATTTCCCATCTCAGT+AGG + tig0034911:23049-23068 Msa1453860:CDS 50.0%
TAGCCACATTAGCAGCGCTT+GGG + tig0034911:23095-23114 Msa1453860:CDS 50.0%
! GCAGCGCTTGGGATGATTTT+AGG + tig0034911:23106-23125 Msa1453860:CDS 50.0%
! GCTTGGGATGATTTTAGGCG+CGG + tig0034911:23111-23130 Msa1453860:CDS 50.0%
GACATTGTAGGTGCTTGCGA+TGG + tig0034911:23307-23326 Msa1453860:intron 50.0%
CATTGTAGGTGCTTGCGATG+GGG + tig0034911:23309-23328 Msa1453860:intron 50.0%
! CGACTTCCCTCAGTTGGTTT+GGG + tig0034911:23338-23357 Msa1453860:intron 50.0%
AAAAGGGGGGACTTCTGCAA+CGG - tig0034911:23373-23392 None:intergenic 50.0%
AAAAAATAAGGCCCGCGCAG+GGG + tig0034911:23445-23464 Msa1453860:CDS 50.0%
!! AGTTATTGGCTCGTCCAACC+CGG - tig0034911:23599-23618 None:intergenic 50.0%
GCCAATAACTCGAACGCGTT+CGG + tig0034911:23609-23628 Msa1453860:CDS 50.0%
! ACCGAACGCGTTCGAGTTAT+TGG - tig0034911:23613-23632 None:intergenic 50.0%
TGGAGTCCGTATCATGGTGA+AGG - tig0034911:23670-23689 None:intergenic 50.0%
GTAGCCACATTAGCAGCGCT+TGG + tig0034911:23094-23113 Msa1453860:CDS 55.0%
! CATCCCAAGCGCTGCTAATG+TGG - tig0034911:23101-23120 None:intergenic 55.0%
GTCGCCCCTCCAACAAGAAA+AGG - tig0034911:23241-23260 None:intergenic 55.0%
GCGACTTCCCTCAGTTGGTT+TGG + tig0034911:23337-23356 Msa1453860:intron 55.0%
!! CTTCCCTCAGTTGGTTTGGG+TGG + tig0034911:23341-23360 Msa1453860:intron 55.0%
AAAGGGGGGACTTCTGCAAC+GGG - tig0034911:23372-23391 None:intergenic 55.0%
TTGGCTGAGCCGTATCTTGC+TGG + tig0034911:23480-23499 Msa1453860:CDS 55.0%
TGGCTGAGCCGTATCTTGCT+GGG + tig0034911:23481-23500 Msa1453860:CDS 55.0%
TGAGCCGTATCTTGCTGGGT+GGG + tig0034911:23485-23504 Msa1453860:CDS 55.0%
GGACCGAGAGCAAGAAAGGA+CGG + tig0034911:23506-23525 Msa1453860:CDS 55.0%
GACCGAGAGCAAGAAAGGAC+GGG + tig0034911:23507-23526 Msa1453860:CDS 55.0%
ACCGAGAGCAAGAAAGGACG+GGG + tig0034911:23508-23527 Msa1453860:CDS 55.0%
GACACGGTCGAGAACATGCA+TGG - tig0034911:23539-23558 None:intergenic 55.0%
TCAACTGTCCCTCGGAGACA+CGG - tig0034911:23555-23574 None:intergenic 55.0%
TCGCTCGTTCAACTGTCCCT+CGG - tig0034911:23563-23582 None:intergenic 55.0%
GAGCGACTCATGAATGCTGC+CGG + tig0034911:23577-23596 Msa1453860:CDS 55.0%
AGCGACTCATGAATGCTGCC+GGG + tig0034911:23578-23597 Msa1453860:CDS 55.0%
ACTCATGAATGCTGCCGGGT+TGG + tig0034911:23582-23601 Msa1453860:CDS 55.0%
CGCGCTCTGCCATAAGAACT+TGG - tig0034911:23690-23709 None:intergenic 55.0%
AGTTCTTATGGCAGAGCGCG+AGG + tig0034911:23690-23709 Msa1453860:CDS 55.0%
TAGGCGCGGCGTATTCCCTT+TGG + tig0034911:23125-23144 Msa1453860:CDS 60.0%
TGTAGGTGCTTGCGATGGGG+CGG + tig0034911:23312-23331 Msa1453860:intron 60.0%
CGGATGCGACTTCCCTCAGT+TGG + tig0034911:23332-23351 Msa1453860:intron 60.0%
!!! GCAGGGGCGCTCACGTTTTT+TGG + tig0034911:23461-23480 Msa1453860:CDS 60.0%
CTGAGCCGTATCTTGCTGGG+TGG + tig0034911:23484-23503 Msa1453860:CDS 60.0%
CGGTCCCACCCAGCAAGATA+CGG - tig0034911:23492-23511 None:intergenic 60.0%
GGTGGGACCGAGAGCAAGAA+AGG + tig0034911:23502-23521 Msa1453860:CDS 60.0%
CCCCCGTCCTTTCTTGCTCT+CGG - tig0034911:23512-23531 None:intergenic 60.0%
CCGAGAGCAAGAAAGGACGG+GGG + tig0034911:23509-23528 Msa1453860:CDS 60.0%
CGAGAGCAAGAAAGGACGGG+GGG + tig0034911:23510-23529 Msa1453860:CDS 60.0%
TGTTCTCGACCGTGTCTCCG+AGG + tig0034911:23543-23562 Msa1453860:CDS 60.0%
GTTCTCGACCGTGTCTCCGA+GGG + tig0034911:23544-23563 Msa1453860:CDS 60.0%
AAAACGTGAGCGCCCCTGCG+CGG - tig0034911:23460-23479 None:intergenic 65.0%
AAACGTGAGCGCCCCTGCGC+GGG - tig0034911:23459-23478 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
tig0034911 gene 22974 23719 22974 ID=Msa1453860;Name=Msa1453860
tig0034911 mRNA 22974 23719 22974 ID=Msa1453860-mRNA-1;Parent=Msa1453860;Name=Msa1453860-mRNA-1;_AED=0.25;_eAED=0.26;_QI=0|0|0|1|0|0.5|2|0|199
tig0034911 exon 22974 23222 22974 ID=Msa1453860-mRNA-1:exon:19337;Parent=Msa1453860-mRNA-1
tig0034911 exon 23369 23719 23369 ID=Msa1453860-mRNA-1:exon:19338;Parent=Msa1453860-mRNA-1
tig0034911 CDS 22974 23222 22974 ID=Msa1453860-mRNA-1:cds;Parent=Msa1453860-mRNA-1
tig0034911 CDS 23369 23719 23369 ID=Msa1453860-mRNA-1:cds;Parent=Msa1453860-mRNA-1
Gene Sequence

>Msa1453860

ATGCCGAATCTCTCTACCATTTCCTTCTCTTCCACTTTGGCCAATATGAGTTCACCTGGTACTAGCAGCTTTATCGGGGAATTTCCCATCTCAGTAGGAGCTTTCCAAAGAAATAGCTTAGTAGCCACATTAGCAGCGCTTGGGATGATTTTAGGCGCGGCGTATTCCCTTTGGCTATATAATCGTGTGGTTTCTGGAAATTTAAAAGCCGATTTCCTCCATAAATTCTCCGATCCAAATGGCAGAGAACCCGTTGCAGAAGTCCCCCCTTTTTTTTATCCATTTCTGTCTTTAGTCTTTAGGGAGCCAAAGCTTGACTTTACTAAAAAAATAAGGCCCGCGCAGGGGCGCTCACGTTTTTTGGCTGAGCCGTATCTTGCTGGGTGGGACCGAGAGCAAGAAAGGACGGGGGGCAACCATGCATGTTCTCGACCGTGTCTCCGAGGGACAGTTGAACGAGCGACTCATGAATGCTGCCGGGTTGGACGAGCCAATAACTCGAACGCGTTCGGTCTGTTTTTTGAGCAAGAATCACAGCGTTACCTTACCTTCACCATGATACGGACTCCAAGTTCTTATGGCAGAGCGCGAGGAGATTGA

Protein sequence

>Msa1453860

MPNLSTISFSSTLANMSSPGTSSFIGEFPISVGAFQRNSLVATLAALGMILGAAYSLWLYNRVVSGNLKADFLHKFSDPNGREPVAEVPPFFYPFLSLVFREPKLDFTKKIRPAQGRSRFLAEPYLAGWDREQERTGGNHACSRPCLRGTVERATHECCRVGRANNSNAFGLFFEQESQRYLTFTMIRTPSSYGRARGD*