AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1453870


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 45 sgRNAs with CRISPR-Local

Find 223 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCCCTTTCTTTAACAAAT+TGG 0.214714 tig0034911:-26324 None:intergenic
ATGGCCGCGGCGATGGAATC+TGG 0.262688 tig0034911:+23843 Msa1453870:CDS
GCTGGAGCTGCCACAGCTTA+TGG 0.304281 tig0034911:+23983 Msa1453870:CDS
ATCCCTTTCTTTAACAAATT+GGG 0.320797 tig0034911:-26323 None:intergenic
CCGCCCGCTTGGCGGGCGGC+AGG 0.332763 tig0034911:+24060 Msa1453870:CDS
GCTCCTGCCGCCCGCCAAGC+GGG 0.366253 tig0034911:-24063 None:intergenic
TCCTTACAGTTCTCTATGCC+GGG 0.375787 tig0034911:-24026 None:intergenic
TGGCGGGCGGCAGGAGCTGC+GGG 0.393725 tig0034911:+24069 Msa1453870:CDS
ATCCTTACAGTTCTCTATGC+CGG 0.406330 tig0034911:-24027 None:intergenic
CAGGGGAATTGCAGTTTCAC+TGG 0.408308 tig0034911:+26355 Msa1453870:CDS
TTGGCGGGCGGCAGGAGCTG+CGG 0.413881 tig0034911:+24068 Msa1453870:CDS
TGTCTACTACCGTATCAGTT+TGG 0.414989 tig0034911:+26387 Msa1453870:CDS
ATACCCAATTTGTTAAAGAA+AGG 0.426291 tig0034911:+26320 Msa1453870:CDS
CAGCTTATGGCTAGAGCAAT+GGG 0.447959 tig0034911:+23996 Msa1453870:CDS
AGCTCCAGCTCGAAACCAAA+GGG 0.448212 tig0034911:-23969 None:intergenic
TACCCAATTTGTTAAAGAAA+GGG 0.454404 tig0034911:+26321 Msa1453870:CDS
GGGCCCCTTTGGTTTCGAGC+TGG 0.465740 tig0034911:+23965 Msa1453870:CDS
CTGCAATTCCCCTGCGGAGT+CGG 0.468714 tig0034911:-26346 None:intergenic
GAACCAGATGGCCGCGGCGA+TGG 0.481859 tig0034911:+23836 None:intergenic
TGGCCGCGGCGATGGAATCT+GGG 0.496216 tig0034911:+23844 Msa1453870:CDS
GACGGCAAAGATGATTTGAC+TGG 0.510517 tig0034911:+26277 Msa1453870:CDS
AGGGGAATTGCAGTTTCACT+GGG 0.514956 tig0034911:+26356 Msa1453870:CDS
GGCGATGGAATCTGGGACGG+AGG 0.517778 tig0034911:+23851 Msa1453870:CDS
AGAAAGGGATCCGACTCCGC+AGG 0.519025 tig0034911:+26336 Msa1453870:CDS
ACAGCTTATGGCTAGAGCAA+TGG 0.524590 tig0034911:+23995 Msa1453870:CDS
ATGGCTAGAGCAATGGGAGG+GGG 0.529861 tig0034911:+24002 Msa1453870:CDS
TATGGCTAGAGCAATGGGAG+GGG 0.529887 tig0034911:+24001 Msa1453870:CDS
TGGGACGAGAAACAAACAAC+GGG 0.535214 tig0034911:+26297 Msa1453870:CDS
GAAAGGGATCCGACTCCGCA+GGG 0.540119 tig0034911:+26337 Msa1453870:CDS
CAGCTCCAGCTCGAAACCAA+AGG 0.542714 tig0034911:-23970 None:intergenic
CGCGGCGATGGAATCTGGGA+CGG 0.582286 tig0034911:+23848 Msa1453870:CDS
ACCGGTGAACCAGATGGCCG+CGG 0.591781 tig0034911:+23830 None:intergenic
GCTATCGTTCCAAACTGATA+CGG 0.594176 tig0034911:-26396 None:intergenic
TTATGGCTAGAGCAATGGGA+GGG 0.604810 tig0034911:+24000 Msa1453870:CDS
CCCCGGCATAGAGAACTGTA+AGG 0.611801 tig0034911:+24025 Msa1453870:CDS
CCTTACAGTTCTCTATGCCG+GGG 0.621235 tig0034911:-24025 None:intergenic
ACGGCAAAGATGATTTGACT+GGG 0.671984 tig0034911:+26278 Msa1453870:CDS
CTTATGGCTAGAGCAATGGG+AGG 0.675758 tig0034911:+23999 Msa1453870:CDS
AAAGGGATCCGACTCCGCAG+GGG 0.687311 tig0034911:+26338 Msa1453870:CDS
CTGGGACGAGAAACAAACAA+CGG 0.690127 tig0034911:+26296 Msa1453870:CDS
ATTGCTCTAGCCATAAGCTG+TGG 0.691371 tig0034911:-23993 None:intergenic
GCTCCAGCTCGAAACCAAAG+GGG 0.703117 tig0034911:-23968 None:intergenic
GTGAAACTGCAATTCCCCTG+CGG 0.730557 tig0034911:-26352 None:intergenic
CGTCCCAGATTCCATCGCCG+CGG 0.737524 tig0034911:-23847 None:intergenic
GGGGAATTGCAGTTTCACTG+GGG 0.756841 tig0034911:+26357 Msa1453870:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAAAATTTATTTAAATTG+GGG + tig0034911:24297-24316 Msa1453870:intron 10.0%
!! AAAAAATTTATTTAAATTGG+GGG + tig0034911:24298-24317 Msa1453870:intron 10.0%
!!! AATTTTGAAATGATTAAAAA+AGG + tig0034911:24453-24472 Msa1453870:intron 10.0%
!!! TTTTTATATTGAAAGAAAAA+TGG + tig0034911:24664-24683 Msa1453870:intron 10.0%
!! ATAAATGAAAATTAAACTAA+CGG + tig0034911:24827-24846 Msa1453870:intron 10.0%
!! AATACAAAAAAAATAGATTA+GGG - tig0034911:24909-24928 None:intergenic 10.0%
!! AAATACAAAAAAAATAGATT+AGG - tig0034911:24910-24929 None:intergenic 10.0%
!!! TTTTTTTTATGTATATAAAG+CGG + tig0034911:24928-24947 Msa1453870:intron 10.0%
!! TTAAAAGCTAAAATAAAAAT+TGG + tig0034911:25867-25886 Msa1453870:intron 10.0%
!! AAAAATTTATTTAAATTGGG+GGG + tig0034911:24299-24318 Msa1453870:intron 15.0%
!! AAAAGTTAAATAAAATCAAG+AGG - tig0034911:24390-24409 None:intergenic 15.0%
!! TCAATAAAATGTAGATAATT+AGG - tig0034911:24483-24502 None:intergenic 15.0%
!!! TTATCTACATTTTATTGATT+TGG + tig0034911:24485-24504 Msa1453870:intron 15.0%
!!! ATTTGTTTTGTGTTTTTTAT+TGG + tig0034911:24584-24603 Msa1453870:intron 15.0%
!!! TTTTTATTGGTTTTGAAAAA+AGG + tig0034911:24597-24616 Msa1453870:intron 15.0%
!! TTTCTCATTTATATTTACAT+TGG + tig0034911:24994-25013 Msa1453870:intron 15.0%
!!! ATTTGAATGATTTTTCAAAT+GGG + tig0034911:25386-25405 Msa1453870:intron 15.0%
!! CGAAAAAAATAAAAACAAAA+TGG + tig0034911:25543-25562 Msa1453870:intron 15.0%
!! ATAAAAACAAAATGGTAAAA+TGG + tig0034911:25551-25570 Msa1453870:intron 15.0%
!!! TTTTAAAATAAAAAGCCAAG+TGG + tig0034911:24620-24639 Msa1453870:intron 20.0%
!!! TTTGAATGTTTTTGTTGTTT+TGG + tig0034911:24800-24819 Msa1453870:intron 20.0%
!!! GTTGATAAAAAATGGAAAAT+TGG + tig0034911:25312-25331 Msa1453870:intron 20.0%
!!! GATTTGAATGATTTTTCAAA+TGG + tig0034911:25385-25404 Msa1453870:intron 20.0%
!! ACTTGAGAAAGAAAAAAAAT+TGG + tig0034911:25445-25464 Msa1453870:intron 20.0%
!!! CTATATTTTTGGACAATTTT+TGG + tig0034911:25477-25496 Msa1453870:intron 20.0%
!! TTTCGTTTAATTACTTCTTT+AGG - tig0034911:25528-25547 None:intergenic 20.0%
!! TAAATTAAAAAAGTGAGAAG+AGG + tig0034911:25652-25671 Msa1453870:intron 20.0%
!!! ACTTTGGTTTTTTTTTTACT+TGG + tig0034911:25799-25818 Msa1453870:intron 20.0%
!! TAGAGTACTACGAATATTTA+GGG - tig0034911:24361-24380 None:intergenic 25.0%
!!! CTACATTTTATTGATTTGGA+AGG + tig0034911:24489-24508 Msa1453870:intron 25.0%
!! AAAATAGATTAGGGATTTTG+TGG - tig0034911:24900-24919 None:intergenic 25.0%
! AATGCAAGAAAATACAAGAA+AGG + tig0034911:25102-25121 Msa1453870:intron 25.0%
! ATGCAAGAAAATACAAGAAA+GGG + tig0034911:25103-25122 Msa1453870:intron 25.0%
!!! GTTCTAAGGTTGATAAAAAA+TGG + tig0034911:25304-25323 Msa1453870:intron 25.0%
! TAAAAATATAAAGAGCGAAG+AGG + tig0034911:25979-25998 Msa1453870:intron 25.0%
! AAAAATATAAAGAGCGAAGA+GGG + tig0034911:25980-25999 Msa1453870:intron 25.0%
! ATCTTAAAATACCTCATGTT+TGG - tig0034911:26018-26037 None:intergenic 25.0%
!!! AATAGTCTTTGACTTTTCAA+AGG - tig0034911:26059-26078 None:intergenic 25.0%
!! ATGTATGAATGTAGGATTTT+AGG + tig0034911:26091-26110 Msa1453870:intron 25.0%
!!! TAGGATTTTAGGTCAAAAAT+TGG + tig0034911:26102-26121 Msa1453870:intron 25.0%
!!! AGGATTTTAGGTCAAAAATT+GGG + tig0034911:26103-26122 Msa1453870:intron 25.0%
! CAACATCTTAAGTCAAAAAT+TGG - tig0034911:26226-26245 None:intergenic 25.0%
!!! ATACCCAATTTGTTAAAGAA+AGG + tig0034911:26320-26339 Msa1453870:CDS 25.0%
!!! TACCCAATTTGTTAAAGAAA+GGG + tig0034911:26321-26340 Msa1453870:CDS 25.0%
! ATCCCTTTCTTTAACAAATT+GGG - tig0034911:26326-26345 None:intergenic 25.0%
! GTAGAGTACTACGAATATTT+AGG - tig0034911:24362-24381 None:intergenic 30.0%
! TTGAAAGAAAAATGGACTCA+AGG + tig0034911:24672-24691 Msa1453870:intron 30.0%
GATCACTTGATTGAAAATCA+AGG + tig0034911:24744-24763 Msa1453870:intron 30.0%
CCAGCAATTAGAATGTATTT+AGG - tig0034911:25021-25040 None:intergenic 30.0%
CCTAAATACATTCTAATTGC+TGG + tig0034911:25018-25037 Msa1453870:intron 30.0%
AGCTCTATTTATAGGTTCTA+AGG + tig0034911:25290-25309 Msa1453870:intron 30.0%
! ATTGGTTTAATGCACTTTGA+TGG + tig0034911:25330-25349 Msa1453870:intron 30.0%
TTGGTTTAATGCACTTTGAT+GGG + tig0034911:25331-25350 Msa1453870:intron 30.0%
!! TTGAATGAGATTGATGGTTT+TGG + tig0034911:25357-25376 Msa1453870:intron 30.0%
TGTAAAAATGGCATGATTGA+TGG + tig0034911:25419-25438 Msa1453870:intron 30.0%
TGTCCAAAAATATAGTGTCA+AGG - tig0034911:25472-25491 None:intergenic 30.0%
TGAAAAGTCAAAGACTATTC+AGG + tig0034911:26060-26079 Msa1453870:intron 30.0%
GAAAAGTCAAAGACTATTCA+GGG + tig0034911:26061-26080 Msa1453870:intron 30.0%
!! GGATTTTAGGTCAAAAATTG+GGG + tig0034911:26104-26123 Msa1453870:intron 30.0%
GACGAAGAAAACTTAAATAG+GGG - tig0034911:26141-26160 None:intergenic 30.0%
GGACGAAGAAAACTTAAATA+GGG - tig0034911:26142-26161 None:intergenic 30.0%
!! CTATTTAAGTTTTCTTCGTC+CGG + tig0034911:26141-26160 Msa1453870:intron 30.0%
AGATTTCATCTTCAAATCTC+CGG - tig0034911:26163-26182 None:intergenic 30.0%
!! AGATGAAATCTTTGAGTTGA+CGG + tig0034911:26172-26191 Msa1453870:intron 30.0%
GATCCCTTTCTTTAACAAAT+TGG - tig0034911:26327-26346 None:intergenic 30.0%
TAAAGACCCCTTAATTCGAA+TGG + tig0034911:23906-23925 Msa1453870:intron 35.0%
AAAGACCCCTTAATTCGAAT+GGG + tig0034911:23907-23926 Msa1453870:intron 35.0%
ATTAAAAGCAAGGACCACTA+CGG + tig0034911:24190-24209 Msa1453870:intron 35.0%
TTTATTTAAATTGGGGGGTC+CGG + tig0034911:24304-24323 Msa1453870:intron 35.0%
! GCAATAAAAATAAAAGCGCG+TGG + tig0034911:24851-24870 Msa1453870:intron 35.0%
! TGGATTTTTGTAGTGACGTT+GGG + tig0034911:24871-24890 Msa1453870:intron 35.0%
! TGACAACTCAAGAGAACTTT+TGG + tig0034911:25244-25263 Msa1453870:intron 35.0%
! TTTGGTGTGTGAAATTGTGA+AGG + tig0034911:25262-25281 Msa1453870:intron 35.0%
AGGATGAGAGCTCTATTTAT+AGG + tig0034911:25282-25301 Msa1453870:intron 35.0%
TGGTTTAATGCACTTTGATG+GGG + tig0034911:25332-25351 Msa1453870:intron 35.0%
GGAGAGTTGACATGTAAAAA+TGG + tig0034911:25407-25426 Msa1453870:intron 35.0%
! GGACCTTGACACTATATTTT+TGG + tig0034911:25466-25485 Msa1453870:intron 35.0%
!! CTTTCTTCCCATTTTTCATC+AGG - tig0034911:25698-25717 None:intergenic 35.0%
!!! TTTTGACTCTGCACTGATTA+TGG + tig0034911:25718-25737 Msa1453870:intron 35.0%
! GGCTTGGAGGAAAAATATTT+TGG + tig0034911:25744-25763 Msa1453870:intron 35.0%
AGTCCAAAATTAAGTGTCCA+GGG - tig0034911:25772-25791 None:intergenic 35.0%
! TTTTGGACTCATTGACACTT+TGG + tig0034911:25783-25802 Msa1453870:intron 35.0%
CAAAGGAAATTTCAGCAGAA+AGG - tig0034911:26042-26061 None:intergenic 35.0%
GTCAGACAATGTATGAATGT+AGG + tig0034911:26083-26102 Msa1453870:intron 35.0%
CGGACGAAGAAAACTTAAAT+AGG - tig0034911:26143-26162 None:intergenic 35.0%
!! GATGAAATCTTTGAGTTGAC+GGG + tig0034911:26173-26192 Msa1453870:intron 35.0%
CATGATGCAATTATGAATGC+AGG + tig0034911:26255-26274 Msa1453870:intron 35.0%
ATGCAATTATGAATGCAGGA+CGG + tig0034911:26259-26278 Msa1453870:intron 35.0%
TCGTAGTATCTCTCTAGATC+TGG + tig0034911:23876-23895 Msa1453870:intron 40.0%
AAGACCCCTTAATTCGAATG+GGG + tig0034911:23908-23927 Msa1453870:intron 40.0%
ATCCTTACAGTTCTCTATGC+CGG - tig0034911:24030-24049 None:intergenic 40.0%
AGTACGATAAATTAGCCCTG+CGG + tig0034911:24161-24180 Msa1453870:intron 40.0%
! GCTTTTAATAAAGCTCCGCA+GGG - tig0034911:24179-24198 None:intergenic 40.0%
! TGCTTTTAATAAAGCTCCGC+AGG - tig0034911:24180-24199 None:intergenic 40.0%
!! GCGGAGCTTTATTAAAAGCA+AGG + tig0034911:24180-24199 Msa1453870:intron 40.0%
TTAAAAGCAAGGACCACTAC+GGG + tig0034911:24191-24210 Msa1453870:intron 40.0%
!!! GCGTCATTTTTTGGAGATCA+AGG + tig0034911:24232-24251 Msa1453870:intron 40.0%
TTATTTAAATTGGGGGGTCC+GGG + tig0034911:24305-24324 Msa1453870:intron 40.0%
!! GGGTTGGTTATATTTAGGGA+AGG + tig0034911:24327-24346 Msa1453870:intron 40.0%
!!! TTTTTTAACCCCGAACTACG+AGG - tig0034911:24554-24573 None:intergenic 40.0%
ACCAATTCTCATTTGCCACT+TGG - tig0034911:24638-24657 None:intergenic 40.0%
! GTGGATTTTTGTAGTGACGT+TGG + tig0034911:24870-24889 Msa1453870:intron 40.0%
! CTTCGGTAATTTTGTCCCTT+CGG - tig0034911:25133-25152 None:intergenic 40.0%
GAAGGGACAAAATTACCGAA+GGG + tig0034911:25132-25151 Msa1453870:intron 40.0%
!!! CTCTCTTTTTTCTGTCCCTT+CGG - tig0034911:25165-25184 None:intergenic 40.0%
TCGTCAACACAAGCTAAATG+AGG + tig0034911:25201-25220 Msa1453870:intron 40.0%
CGTCAACACAAGCTAAATGA+GGG + tig0034911:25202-25221 Msa1453870:intron 40.0%
GGGGATTTGAATGAGATTGA+TGG + tig0034911:25351-25370 Msa1453870:intron 40.0%
AACGCGTTCCTGATGAAAAA+TGG + tig0034911:25687-25706 Msa1453870:intron 40.0%
ACGCGTTCCTGATGAAAAAT+GGG + tig0034911:25688-25707 Msa1453870:intron 40.0%
!! GGAAAAATATTTTGGACCCC+TGG + tig0034911:25752-25771 Msa1453870:intron 40.0%
GTCCAAAATTAAGTGTCCAG+GGG - tig0034911:25771-25790 None:intergenic 40.0%
GAGTCCAAAATTAAGTGTCC+AGG - tig0034911:25773-25792 None:intergenic 40.0%
ACGGCAAAGATGATTTGACT+GGG + tig0034911:26278-26297 Msa1453870:CDS 40.0%
TGTCTACTACCGTATCAGTT+TGG + tig0034911:26387-26406 Msa1453870:CDS 40.0%
AGACCCCTTAATTCGAATGG+GGG + tig0034911:23909-23928 Msa1453870:intron 45.0%
TCAGCCCCCATTCGAATTAA+GGG - tig0034911:23916-23935 None:intergenic 45.0%
ATTGCTCTAGCCATAAGCTG+TGG - tig0034911:23996-24015 None:intergenic 45.0%
ACAGCTTATGGCTAGAGCAA+TGG + tig0034911:23995-24014 Msa1453870:CDS 45.0%
CAGCTTATGGCTAGAGCAAT+GGG + tig0034911:23996-24015 Msa1453870:CDS 45.0%
TTATGGCTAGAGCAATGGGA+GGG + tig0034911:24000-24019 Msa1453870:CDS 45.0%
TCCTTACAGTTCTCTATGCC+GGG - tig0034911:24029-24048 None:intergenic 45.0%
CAAAAAATGACGCGACACCT+TGG - tig0034911:24225-24244 None:intergenic 45.0%
!!! CAAGGTGTCGCGTCATTTTT+TGG + tig0034911:24223-24242 Msa1453870:intron 45.0%
TATTTAAATTGGGGGGTCCG+GGG + tig0034911:24306-24325 Msa1453870:intron 45.0%
!! TCCGGGGGTTGGTTATATTT+AGG + tig0034911:24322-24341 Msa1453870:intron 45.0%
CCCTAAATATAACCAACCCC+CGG - tig0034911:24326-24345 None:intergenic 45.0%
!! CCGGGGGTTGGTTATATTTA+GGG + tig0034911:24323-24342 Msa1453870:intron 45.0%
!! TACGAGGTTTTGATCCCTCA+CGG - tig0034911:24538-24557 None:intergenic 45.0%
GGGATCAAAACCTCGTAGTT+CGG + tig0034911:24541-24560 Msa1453870:intron 45.0%
GGATCAAAACCTCGTAGTTC+GGG + tig0034911:24542-24561 Msa1453870:intron 45.0%
GATCAAAACCTCGTAGTTCG+GGG + tig0034911:24543-24562 Msa1453870:intron 45.0%
GCCAAGTGGCAAATGAGAAT+TGG + tig0034911:24634-24653 Msa1453870:intron 45.0%
TACAAGAAAGGGACAACCGA+AGG + tig0034911:25114-25133 Msa1453870:intron 45.0%
ACAAGAAAGGGACAACCGAA+GGG + tig0034911:25115-25134 Msa1453870:intron 45.0%
CGAAGGGACAAAATTACCGA+AGG + tig0034911:25131-25150 Msa1453870:intron 45.0%
!! ACTCTGCACTGATTATGGCA+TGG + tig0034911:25723-25742 Msa1453870:intron 45.0%
! GACCCCTGGACACTTAATTT+TGG + tig0034911:25766-25785 Msa1453870:intron 45.0%
GACGGCAAAGATGATTTGAC+TGG + tig0034911:26277-26296 Msa1453870:CDS 45.0%
CTGGGACGAGAAACAAACAA+CGG + tig0034911:26296-26315 Msa1453870:CDS 45.0%
TGGGACGAGAAACAAACAAC+GGG + tig0034911:26297-26316 Msa1453870:CDS 45.0%
AGGGGAATTGCAGTTTCACT+GGG + tig0034911:26356-26375 Msa1453870:CDS 45.0%
!! TTGAAAAAATTTATTTAAAT+TGG + tig0034911:24295-24314 Msa1453870:intron 5.0%
!! TGAAAAAATTTATTTAAATT+GGG + tig0034911:24296-24315 Msa1453870:intron 5.0%
!! TTATAAAATTAAATAAAAGA+TGG + tig0034911:25905-25924 Msa1453870:intron 5.0%
CAGCCCCCATTCGAATTAAG+GGG - tig0034911:23915-23934 None:intergenic 50.0%
GTCAGCCCCCATTCGAATTA+AGG - tig0034911:23917-23936 None:intergenic 50.0%
AGCTCCAGCTCGAAACCAAA+GGG - tig0034911:23972-23991 None:intergenic 50.0%
CTTATGGCTAGAGCAATGGG+AGG + tig0034911:23999-24018 Msa1453870:CDS 50.0%
TATGGCTAGAGCAATGGGAG+GGG + tig0034911:24001-24020 Msa1453870:CDS 50.0%
CCTTACAGTTCTCTATGCCG+GGG - tig0034911:24028-24047 None:intergenic 50.0%
ATTTAAATTGGGGGGTCCGG+GGG + tig0034911:24307-24326 Msa1453870:intron 50.0%
!! ACGAGGTTTTGATCCCTCAC+GGG - tig0034911:24537-24556 None:intergenic 50.0%
ACCGAAGGGACTTATTCCGA+AGG + tig0034911:25146-25165 Msa1453870:intron 50.0%
CCCTTCGGAATAAGTCCCTT+CGG - tig0034911:25150-25169 None:intergenic 50.0%
CCGAAGGGACTTATTCCGAA+GGG + tig0034911:25147-25166 Msa1453870:intron 50.0%
!! GCACTGATTATGGCATGGCT+TGG + tig0034911:25728-25747 Msa1453870:intron 50.0%
! CTGATTATGGCATGGCTTGG+AGG + tig0034911:25731-25750 Msa1453870:intron 50.0%
! CCTCATGTTTGGTTCCGAGT+CGG - tig0034911:26007-26026 None:intergenic 50.0%
GTGAAACTGCAATTCCCCTG+CGG - tig0034911:26355-26374 None:intergenic 50.0%
CAGGGGAATTGCAGTTTCAC+TGG + tig0034911:26355-26374 Msa1453870:CDS 50.0%
GGGGAATTGCAGTTTCACTG+GGG + tig0034911:26357-26376 Msa1453870:CDS 50.0%
GCTCCAGCTCGAAACCAAAG+GGG - tig0034911:23971-23990 None:intergenic 55.0%
CAGCTCCAGCTCGAAACCAA+AGG - tig0034911:23973-23992 None:intergenic 55.0%
ATGGCTAGAGCAATGGGAGG+GGG + tig0034911:24002-24021 Msa1453870:CDS 55.0%
CCCCGGCATAGAGAACTGTA+AGG + tig0034911:24025-24044 Msa1453870:CDS 55.0%
AAAGCAAGGACCACTACGGG+AGG + tig0034911:24194-24213 Msa1453870:intron 55.0%
! CTTGGTTTGACCTCCCGTAG+TGG - tig0034911:24207-24226 None:intergenic 55.0%
CACTACGGGAGGTCAAACCA+AGG + tig0034911:24205-24224 Msa1453870:intron 55.0%
TTGATCCCTCACGGGTACGT+AGG - tig0034911:24529-24548 None:intergenic 55.0%
!! CCGACTCGGAACCAAACATG+AGG + tig0034911:26004-26023 Msa1453870:intron 55.0%
GCTGGAGCTGCCACAGCTTA+TGG + tig0034911:23983-24002 Msa1453870:CDS 60.0%
AGGCCAACAGCCCAGTGAAC+CGG + tig0034911:24104-24123 Msa1453870:intron 60.0%
CTCTGCCTACGTACCCGTGA+GGG + tig0034911:24521-24540 Msa1453870:intron 60.0%
AGAGCGAAGAGGGTCCGACT+CGG + tig0034911:25990-26009 Msa1453870:intron 60.0%
AGAAAGGGATCCGACTCCGC+AGG + tig0034911:26336-26355 Msa1453870:CDS 60.0%
GAAAGGGATCCGACTCCGCA+GGG + tig0034911:26337-26356 Msa1453870:CDS 60.0%
AAAGGGATCCGACTCCGCAG+GGG + tig0034911:26338-26357 Msa1453870:CDS 60.0%
CTGCAATTCCCCTGCGGAGT+CGG - tig0034911:26349-26368 None:intergenic 60.0%
ATGGCCGCGGCGATGGAATC+TGG + tig0034911:23843-23862 Msa1453870:CDS 65.0%
TGGCCGCGGCGATGGAATCT+GGG + tig0034911:23844-23863 Msa1453870:CDS 65.0%
CGTCCCAGATTCCATCGCCG+CGG - tig0034911:23850-23869 None:intergenic 65.0%
CGCGGCGATGGAATCTGGGA+CGG + tig0034911:23848-23867 Msa1453870:CDS 65.0%
! GGCGATGGAATCTGGGACGG+AGG + tig0034911:23851-23870 Msa1453870:CDS 65.0%
GCTGACCACTGAGCCCACTC+TGG + tig0034911:23931-23950 Msa1453870:intron 65.0%
! GGGCCCCTTTGGTTTCGAGC+TGG + tig0034911:23965-23984 Msa1453870:CDS 65.0%
AGGAGCTGCGGGCAAGTGCT+TGG + tig0034911:24080-24099 Msa1453870:intron 65.0%
GCTGCGGGCAAGTGCTTGGT+AGG + tig0034911:24084-24103 Msa1453870:intron 65.0%
GGCCAACAGCCCAGTGAACC+GGG + tig0034911:24105-24124 Msa1453870:intron 65.0%
CAACAGCCCAGTGAACCGGG+CGG + tig0034911:24108-24127 Msa1453870:intron 65.0%
AACAGCCCAGTGAACCGGGC+GGG + tig0034911:24109-24128 Msa1453870:intron 65.0%
AAATTGGGGGGTCCGGGGGT+TGG + tig0034911:24311-24330 Msa1453870:intron 65.0%
CCTCACGGGTACGTAGGCAG+AGG - tig0034911:24523-24542 None:intergenic 65.0%
CCTCTGCCTACGTACCCGTG+AGG + tig0034911:24520-24539 Msa1453870:intron 65.0%
! CGAGGCCAGAGTGGGCTCAG+TGG - tig0034911:23939-23958 None:intergenic 70.0%
TGAGCCCACTCTGGCCTCGC+AGG + tig0034911:23940-23959 Msa1453870:intron 70.0%
GAGCCCACTCTGGCCTCGCA+GGG + tig0034911:23941-23960 Msa1453870:intron 70.0%
AGAGCAATGGGAGGGGGCCC+CGG + tig0034911:24008-24027 Msa1453870:CDS 70.0%
TAACGAGCGCTCCGCCCGCT+TGG + tig0034911:24049-24068 Msa1453870:CDS 70.0%
CGCCCGGTTCACTGGGCTGT+TGG - tig0034911:24110-24129 None:intergenic 70.0%
ACAGCCCAGTGAACCGGGCG+GGG + tig0034911:24110-24129 Msa1453870:intron 70.0%
AGTGCCCCGCCCGGTTCACT+GGG - tig0034911:24117-24136 None:intergenic 70.0%
CTTTCGTCGAGTGCCCCGCC+CGG - tig0034911:24126-24145 None:intergenic 70.0%
! GGGCGGGGCACTCGACGAAA+GGG + tig0034911:24125-24144 Msa1453870:intron 70.0%
! GGCGGGGCACTCGACGAAAG+GGG + tig0034911:24126-24145 Msa1453870:intron 70.0%
! GCGGGGCACTCGACGAAAGG+GGG + tig0034911:24127-24146 Msa1453870:intron 70.0%
CCGCCCTGCGAGGCCAGAGT+GGG - tig0034911:23947-23966 None:intergenic 75.0%
CCCACTCTGGCCTCGCAGGG+CGG + tig0034911:23944-23963 Msa1453870:intron 75.0%
CCACTCTGGCCTCGCAGGGC+GGG + tig0034911:23945-23964 Msa1453870:intron 75.0%
AGCTCCTGCCGCCCGCCAAG+CGG - tig0034911:24067-24086 None:intergenic 75.0%
! TTGGCGGGCGGCAGGAGCTG+CGG + tig0034911:24068-24087 Msa1453870:CDS 75.0%
GAGTGCCCCGCCCGGTTCAC+TGG - tig0034911:24118-24137 None:intergenic 75.0%
! CGGGCGGGGCACTCGACGAA+AGG + tig0034911:24124-24143 Msa1453870:intron 75.0%
! CCCGCCCTGCGAGGCCAGAG+TGG - tig0034911:23948-23967 None:intergenic 80.0%
! CCAAAGGGGCCCGCCCTGCG+AGG - tig0034911:23957-23976 None:intergenic 80.0%
! CCTCGCAGGGCGGGCCCCTT+TGG + tig0034911:23954-23973 Msa1453870:intron 80.0%
! CGAGCGCTCCGCCCGCTTGG+CGG + tig0034911:24052-24071 Msa1453870:CDS 80.0%
! GAGCGCTCCGCCCGCTTGGC+GGG + tig0034911:24053-24072 Msa1453870:CDS 80.0%
! GCTCCTGCCGCCCGCCAAGC+GGG - tig0034911:24066-24085 None:intergenic 80.0%
!! TGGCGGGCGGCAGGAGCTGC+GGG + tig0034911:24069-24088 Msa1453870:CDS 80.0%
! CGCTCCGCCCGCTTGGCGGG+CGG + tig0034911:24056-24075 Msa1453870:CDS 85.0%
! CCTGCCGCCCGCCAAGCGGG+CGG - tig0034911:24063-24082 None:intergenic 85.0%
!! CCGCCCGCTTGGCGGGCGGC+AGG + tig0034911:24060-24079 Msa1453870:CDS 90.0%
Chromosome Type Strat End Strand Name
tig0034911 gene 23843 26417 23843 ID=Msa1453870;Name=Msa1453870
tig0034911 mRNA 23843 26417 23843 ID=Msa1453870-mRNA-1;Parent=Msa1453870;Name=Msa1453870-mRNA-1;_AED=0.93;_eAED=1.00;_QI=0|0|0|0.33|1|1|3|0|102
tig0034911 exon 23843 23877 23843 ID=Msa1453870-mRNA-1:exon:19345;Parent=Msa1453870-mRNA-1
tig0034911 exon 23962 24094 23962 ID=Msa1453870-mRNA-1:exon:19346;Parent=Msa1453870-mRNA-1
tig0034911 exon 26277 26417 26277 ID=Msa1453870-mRNA-1:exon:19347;Parent=Msa1453870-mRNA-1
tig0034911 CDS 23843 23877 23843 ID=Msa1453870-mRNA-1:cds;Parent=Msa1453870-mRNA-1
tig0034911 CDS 23962 24094 23962 ID=Msa1453870-mRNA-1:cds;Parent=Msa1453870-mRNA-1
tig0034911 CDS 26277 26417 26277 ID=Msa1453870-mRNA-1:cds;Parent=Msa1453870-mRNA-1
Gene Sequence

>Msa1453870

ATGGCCGCGGCGATGGAATCTGGGACGGAGGACTCGGCGGGCCCCTTTGGTTTCGAGCTGGAGCTGCCACAGCTTATGGCTAGAGCAATGGGAGGGGGCCCCGGCATAGAGAACTGTAAGGATAACGAGCGCTCCGCCCGCTTGGCGGGCGGCAGGAGCTGCGGGCAAGACGGCAAAGATGATTTGACTGGGACGAGAAACAAACAACGGGATACCCAATTTGTTAAAGAAAGGGATCCGACTCCGCAGGGGAATTGCAGTTTCACTGGGGAAGAGATTGTCTACTACCGTATCAGTTTGGAACGATAG

Protein sequence

>Msa1453870

MAAAMESGTEDSAGPFGFELELPQLMARAMGGGPGIENCKDNERSARLAGGRSCGQDGKDDLTGTRNKQRDTQFVKERDPTPQGNCSFTGEEIVYYRISLER*