AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1396010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1396010 MtrunA17_Chr7g0254951 92.727 220 12 1 21 236 1 220 1.46e-138 389
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1396010 AT3G56820.1 54.673 214 75 5 49 245 12 220 1.32e-70 216
Msa1396010 AT3G56820.3 50.935 214 67 6 49 245 12 204 2.89e-59 186
Msa1396010 AT3G56820.2 50.340 147 53 3 49 178 12 155 4.39e-38 131

Find 97 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTAAGTTGTTCTCTTTAT+AGG 0.149046 tig0021982:+36269 Msa1396010:exon
GCACTTTCTTCCAGCATTTC+AGG 0.170527 tig0021982:+34582 Msa1396010:CDS
TTTATGCCTTAGTCTATTAA+AGG 0.217854 tig0021982:+36486 Msa1396010:three_prime_UTR
GGGTGCTGCTATCAAGTTTA+GGG 0.274684 tig0021982:+34707 Msa1396010:CDS
GTTCGGAGTGCTTACTTTAT+AGG 0.282848 tig0021982:+36185 Msa1396010:CDS
CTCTTCATCTTGGTGAAATT+CGG 0.295807 tig0021982:+34625 Msa1396010:CDS
GGGGTGCTGCTATCAAGTTT+AGG 0.298006 tig0021982:+34706 Msa1396010:CDS
TAGCTCTAATGTCGTCGTTT+GGG 0.317927 tig0021982:-34479 None:intergenic
AGGATGGTGGTATGTTGATT+TGG 0.324496 tig0021982:-34296 None:intergenic
CAAGTACGGACTTTAATGTT+TGG 0.354005 tig0021982:+36240 Msa1396010:CDS
CCATCTTCCTCTTCCTCGCT+TGG 0.355448 tig0021982:+34409 Msa1396010:CDS
GTGGTGGTGGTGGTTCTCTC+TGG 0.360872 tig0021982:-34210 None:intergenic
ACTTGTGCTTCTCTTCATCT+TGG 0.370893 tig0021982:+34615 Msa1396010:CDS
TTCCTTCCTTTAATAGACTA+AGG 0.372920 tig0021982:-36492 None:intergenic
AACAGCGAGGTAACTGATAT+TGG 0.393984 tig0021982:+36074 Msa1396010:CDS
CATCTTGGTGAAATTCGGCC+TGG 0.396019 tig0021982:+34630 Msa1396010:CDS
TATAGTTTATGATACTATCT+TGG 0.398381 tig0021982:-36211 None:intergenic
TTAGCTCTAATGTCGTCGTT+TGG 0.404910 tig0021982:-34480 None:intergenic
TTTACAATCAGTACAAGTTC+AGG 0.405213 tig0021982:-36536 None:intergenic
GGAGAGAGTGAGGTCTTTGA+AGG 0.412613 tig0021982:-34374 None:intergenic
GAAGAAAGTGCGAGAGCAAT+AGG 0.413504 tig0021982:-34570 None:intergenic
AAGAGTAAAGAATACACTAT+TGG 0.415723 tig0021982:+34166 Msa1396010:five_prime_UTR
CCGTTGAAACATGGCGGAAG+TGG 0.425519 tig0021982:-34325 None:intergenic
TTATTATATAGACATCTAAA+AGG 0.426582 tig0021982:-36385 None:intergenic
AACAGCTCCACCTGAAATGC+TGG 0.433121 tig0021982:-34592 None:intergenic
GGAAGATGGAGAGGACGAAA+GGG 0.435556 tig0021982:-34395 None:intergenic
AGGAAGATGGAGAGGACGAA+AGG 0.436973 tig0021982:-34396 None:intergenic
CCACGATTATCCTTGGCAAT+AGG 0.438610 tig0021982:-34534 None:intergenic
GTAAATAGGCAACGCAGATT+AGG 0.445935 tig0021982:-36612 None:intergenic
GGTGGTATGTTGATTTGGAT+TGG 0.448511 tig0021982:-34291 None:intergenic
GGAATGAGGTGACGAGGGAG+GGG 0.455393 tig0021982:-34353 None:intergenic
ATCTTGGTGAAATTCGGCCT+GGG 0.462049 tig0021982:+34631 Msa1396010:CDS
TGGTCTTCGCGGTGTTGCGC+CGG 0.464153 tig0021982:-34271 None:intergenic
GAGAGATCCAAGCGAGGAAG+AGG 0.466239 tig0021982:-34416 None:intergenic
TGACATTGCGTTGCTGCTAC+TGG 0.469672 tig0021982:-34241 None:intergenic
TTTGGGGAAGAGAGAGGGAG+TGG 0.476012 tig0021982:-34462 None:intergenic
GCTGCTACTGGTGGTGGTGG+TGG 0.477279 tig0021982:-34229 None:intergenic
AAAAGAAAGACGGAATGAAA+GGG 0.482053 tig0021982:+36456 Msa1396010:three_prime_UTR
AGGAATGAGGTGACGAGGGA+GGG 0.496689 tig0021982:-34354 None:intergenic
AAAGCATGTGCAGTATGAGC+AGG 0.498771 tig0021982:-36146 None:intergenic
CCAAGCGAGGAAGAGGAAGA+TGG 0.501259 tig0021982:-34409 None:intergenic
TAGTGAACATAGATCCGGTT+CGG 0.501356 tig0021982:+36168 Msa1396010:CDS
AACGCAATGTCATCAACAAC+CGG 0.502607 tig0021982:+34252 Msa1396010:CDS
TTAGTCTATTAAAGGAAGGA+AGG 0.509234 tig0021982:+36494 Msa1396010:three_prime_UTR
TGCGTTGCTGCTACTGGTGG+TGG 0.511327 tig0021982:-34235 None:intergenic
ATAAAGTAAGCACTCCGAAC+CGG 0.512043 tig0021982:-36182 None:intergenic
TGCTTTAGTGAACATAGATC+CGG 0.514575 tig0021982:+36163 Msa1396010:CDS
CTTTCCTTCTCCTATTGCCA+AGG 0.517574 tig0021982:+34524 Msa1396010:CDS
CATGGCGGAAGTGGAGGAGG+AGG 0.520349 tig0021982:-34316 None:intergenic
TGATTTCCTCTCAACTTCCC+AGG 0.521431 tig0021982:-34648 None:intergenic
GTGAGGTCTTTGAAGGAATG+AGG 0.525795 tig0021982:-34367 None:intergenic
AAACATGGCGGAAGTGGAGG+AGG 0.531130 tig0021982:-34319 None:intergenic
CCACTTCCGCCATGTTTCAA+CGG 0.538029 tig0021982:+34325 Msa1396010:CDS
AATCCAACCACGATTATCCT+TGG 0.538228 tig0021982:-34541 None:intergenic
AACATTAAAGTCCGTACTTG+AGG 0.548423 tig0021982:-36237 None:intergenic
ATGAGGTGACGAGGGAGGGG+CGG 0.548655 tig0021982:-34350 None:intergenic
AAACTATAATGCCTCAAGTA+CGG 0.551251 tig0021982:+36226 Msa1396010:CDS
CTTTCTTCCAGCATTTCAGG+TGG 0.551639 tig0021982:+34585 Msa1396010:CDS
AACTTGTACTGATTGTAAAG+AGG 0.554257 tig0021982:+36540 Msa1396010:three_prime_UTR
TTCATCAAGAGGAATGTGAT+GGG 0.554812 tig0021982:-36296 None:intergenic
GGCTATGCTGAGGTGACCAT+TGG 0.556044 tig0021982:+36095 Msa1396010:CDS
AGCTCTAATGTCGTCGTTTG+GGG 0.556431 tig0021982:-34478 None:intergenic
TGCCTTAGTCTATTAAAGGA+AGG 0.558946 tig0021982:+36490 Msa1396010:three_prime_UTR
TTGAAACATGGCGGAAGTGG+AGG 0.573268 tig0021982:-34322 None:intergenic
TTGCCAAGGATAATCGTGGT+TGG 0.573821 tig0021982:+34538 Msa1396010:CDS
CGTCGTTTGGGGAAGAGAGA+GGG 0.576409 tig0021982:-34467 None:intergenic
GGGGCGGATCCGTTGAAACA+TGG 0.576935 tig0021982:-34334 None:intergenic
GCGGAAGTGGAGGAGGAGGA+TGG 0.577933 tig0021982:-34312 None:intergenic
TTTCATCAAGAGGAATGTGA+TGG 0.579388 tig0021982:-36297 None:intergenic
GGCAATAGGAGAAGGAAAGC+GGG 0.581159 tig0021982:-34520 None:intergenic
AAGGAATGAGGTGACGAGGG+AGG 0.581491 tig0021982:-34355 None:intergenic
CGAGGAAGAGGAAGATGGAG+AGG 0.582911 tig0021982:-34404 None:intergenic
AGGACGAAAGGGAGAGAGTG+AGG 0.583353 tig0021982:-34384 None:intergenic
AACAACCTCATCTCTGCCAA+TGG 0.586486 tig0021982:-36111 None:intergenic
TAGTCTATTAAAGGAAGGAA+GGG 0.588168 tig0021982:+36495 Msa1396010:three_prime_UTR
TTATCCTTGGCAATAGGAGA+AGG 0.589934 tig0021982:-34528 None:intergenic
ACATCTTAGATTTCATCAAG+AGG 0.594395 tig0021982:-36307 None:intergenic
GAAGTGGAGGAGGAGGATGG+TGG 0.598353 tig0021982:-34309 None:intergenic
TTGATTTGGATTGGTCTTCG+CGG 0.598960 tig0021982:-34282 None:intergenic
GTTGCTGCTACTGGTGGTGG+TGG 0.603371 tig0021982:-34232 None:intergenic
TCGTCGTTTGGGGAAGAGAG+AGG 0.610938 tig0021982:-34468 None:intergenic
AAAGAAAGACGGAATGAAAG+GGG 0.614950 tig0021982:+36457 Msa1396010:three_prime_UTR
ATTCGGCCTGGGAAGTTGAG+AGG 0.620512 tig0021982:+34642 Msa1396010:CDS
GATGAGAAGAGCGATATGAA+CGG 0.633747 tig0021982:-34440 None:intergenic
CCTATTGCCAAGGATAATCG+TGG 0.641137 tig0021982:+34534 Msa1396010:CDS
AAGCATGTGCAGTATGAGCA+GGG 0.642060 tig0021982:-36145 None:intergenic
TGGCAATAGGAGAAGGAAAG+CGG 0.647675 tig0021982:-34521 None:intergenic
TTTGAAGGAATGAGGTGACG+AGG 0.649908 tig0021982:-34359 None:intergenic
TTGAAGGAATGAGGTGACGA+GGG 0.664590 tig0021982:-34358 None:intergenic
TCATCAAGAGGAATGTGATG+GGG 0.666815 tig0021982:-36295 None:intergenic
CATTGCGTTGCTGCTACTGG+TGG 0.668867 tig0021982:-34238 None:intergenic
AACTGATATTGGCTATGCTG+AGG 0.679963 tig0021982:+36085 Msa1396010:CDS
AAGAAAGACGGAATGAAAGG+GGG 0.685351 tig0021982:+36458 Msa1396010:three_prime_UTR
GGTGACCATTGGCAGAGATG+AGG 0.708921 tig0021982:+36106 Msa1396010:CDS
AACGGAGAGAGATCCAAGCG+AGG 0.715907 tig0021982:-34422 None:intergenic
GCGGATCCGTTGAAACATGG+CGG 0.736324 tig0021982:-34331 None:intergenic
TTTGCAGGTGGAAAACAGCG+AGG 0.739005 tig0021982:+36061 Msa1396010:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCTTTTTTTTTTTTTGTTT+TGG - tig0021982:34128-34147 None:intergenic 10.0%
!!! TAATATTATTATGATTTTGT+TGG + tig0021982:36340-36359 Msa1396010:three_prime_UTR 10.0%
!!! AAATATCAACTTAAGTTTTT+TGG + tig0021982:35812-35831 Msa1396010:intron 15.0%
!! TTATTATATAGACATCTAAA+AGG - tig0021982:36388-36407 None:intergenic 15.0%
!! AGATAAAATGCTAGTTATAT+TGG + tig0021982:34794-34813 Msa1396010:intron 20.0%
!! AACTATCAATTATACATCAA+TGG - tig0021982:34891-34910 None:intergenic 20.0%
!!! TATTTTGCAAACCAATTTTA+TGG + tig0021982:35410-35429 Msa1396010:intron 20.0%
!! AAAAGATTCTTCATTTCATT+GGG - tig0021982:35989-36008 None:intergenic 20.0%
!! AAAAAGATTCTTCATTTCAT+TGG - tig0021982:35990-36009 None:intergenic 20.0%
!! TATAGTTTATGATACTATCT+TGG - tig0021982:36214-36233 None:intergenic 20.0%
! AAGAGTAAAGAATACACTAT+TGG + tig0021982:34166-34185 Msa1396010:five_prime_UTR 25.0%
!! TAATGAGACTTTTGTTCTTT+GGG + tig0021982:34686-34705 Msa1396010:CDS 25.0%
! ATTAATTTAAGAGAGAGGAA+AGG - tig0021982:34741-34760 None:intergenic 25.0%
! TCCTCTCTCTTAAATTAATT+TGG + tig0021982:34742-34761 Msa1396010:intron 25.0%
! ACCAAATTAATTTAAGAGAG+AGG - tig0021982:34746-34765 None:intergenic 25.0%
! TTTCAAAACCATGTTTGAAT+TGG - tig0021982:34775-34794 None:intergenic 25.0%
! TGAATGTTCGAATTAACATT+TGG + tig0021982:34824-34843 Msa1396010:intron 25.0%
! TAGTCCTTAACTATTATGTT+AGG + tig0021982:35049-35068 Msa1396010:intron 25.0%
! ACAACCTAACATAATAGTTA+AGG - tig0021982:35056-35075 None:intergenic 25.0%
! AGTAATAACTATAGTATACC+TGG - tig0021982:35146-35165 None:intergenic 25.0%
!!! GTTATTACTGAAAAAGTGTT+TGG + tig0021982:35157-35176 Msa1396010:intron 25.0%
! TAGCATCACTTTATATCATT+GGG - tig0021982:35221-35240 None:intergenic 25.0%
! TTAGCATCACTTTATATCAT+TGG - tig0021982:35222-35241 None:intergenic 25.0%
! AAGCAAACACTAAAAATGTT+TGG + tig0021982:35383-35402 Msa1396010:intron 25.0%
! CAAATATAAGTTTGCAAACT+AGG - tig0021982:35608-35627 None:intergenic 25.0%
! ATATTTAGTATGTACATGTG+TGG - tig0021982:35798-35817 None:intergenic 25.0%
!! TTTCATTGGGATTGTAATTT+TGG - tig0021982:35976-35995 None:intergenic 25.0%
!!! TGAAGAATCTTTTTTGTTGT+TGG + tig0021982:35996-36015 Msa1396010:intron 25.0%
!!! CTTTAAGTTGTTCTCTTTAT+AGG + tig0021982:36269-36288 Msa1396010:exon 25.0%
! TTTATGCCTTAGTCTATTAA+AGG + tig0021982:36486-36505 Msa1396010:three_prime_UTR 25.0%
!! ACATTTTCAGAGTTTAACAA+GGG - tig0021982:36588-36607 None:intergenic 25.0%
! GTAATGAGACTTTTGTTCTT+TGG + tig0021982:34685-34704 Msa1396010:CDS 30.0%
! AATGAGACTTTTGTTCTTTG+GGG + tig0021982:34687-34706 Msa1396010:CDS 30.0%
TTGGTTCCTCTTATTCTATT+TGG + tig0021982:34843-34862 Msa1396010:intron 30.0%
TACACTCCAAATAGAATAAG+AGG - tig0021982:34852-34871 None:intergenic 30.0%
!!! GATTTTAACTTGTTGACTTG+TGG + tig0021982:35077-35096 Msa1396010:intron 30.0%
ATTGTGTTTGATGTTATACC+AGG + tig0021982:35125-35144 Msa1396010:intron 30.0%
CATTAATAGATCCTAGTTAC+AGG - tig0021982:35291-35310 None:intergenic 30.0%
AAGATGAAAATTACGCTCTA+TGG + tig0021982:35334-35353 Msa1396010:intron 30.0%
AATACTTATTGATCCAATGG+TGG - tig0021982:35483-35502 None:intergenic 30.0%
ACATATCACATTTGATCCAA+TGG - tig0021982:35507-35526 None:intergenic 30.0%
TTGCTATGCAAAATGAAGTT+GGG - tig0021982:35632-35651 None:intergenic 30.0%
TTAAAATGGAAACAAAGGGA+AGG - tig0021982:35655-35674 None:intergenic 30.0%
! CGGTTTAAAATGGAAACAAA+GGG - tig0021982:35659-35678 None:intergenic 30.0%
! TCGGTTTAAAATGGAAACAA+AGG - tig0021982:35660-35679 None:intergenic 30.0%
!!! TTGTTCAGTTGTTTGGTTTT+CGG - tig0021982:35721-35740 None:intergenic 30.0%
TGCTGTGTTAAAATAAGCTA+CGG + tig0021982:35751-35770 Msa1396010:intron 30.0%
!! CTCAAAATGATTAGCTTATG+CGG + tig0021982:35911-35930 Msa1396010:intron 30.0%
AAACTATAATGCCTCAAGTA+CGG + tig0021982:36226-36245 Msa1396010:CDS 30.0%
ACATCTTAGATTTCATCAAG+AGG - tig0021982:36310-36329 None:intergenic 30.0%
! ACTCAGTATGAGCATTTTAT+AGG + tig0021982:36428-36447 Msa1396010:three_prime_UTR 30.0%
! CTCAGTATGAGCATTTTATA+GGG + tig0021982:36429-36448 Msa1396010:three_prime_UTR 30.0%
ATAGGGTACAAAAAGAAAGA+CGG + tig0021982:36446-36465 Msa1396010:three_prime_UTR 30.0%
AAAAAGAAAGACGGAATGAA+AGG + tig0021982:36455-36474 Msa1396010:three_prime_UTR 30.0%
AAAAGAAAGACGGAATGAAA+GGG + tig0021982:36456-36475 Msa1396010:three_prime_UTR 30.0%
TTCCTTCCTTTAATAGACTA+AGG - tig0021982:36495-36514 None:intergenic 30.0%
TTAGTCTATTAAAGGAAGGA+AGG + tig0021982:36494-36513 Msa1396010:three_prime_UTR 30.0%
TAGTCTATTAAAGGAAGGAA+GGG + tig0021982:36495-36514 Msa1396010:three_prime_UTR 30.0%
TTTACAATCAGTACAAGTTC+AGG - tig0021982:36539-36558 None:intergenic 30.0%
!! AACTTGTACTGATTGTAAAG+AGG + tig0021982:36540-36559 Msa1396010:three_prime_UTR 30.0%
! CACATTTTCAGAGTTTAACA+AGG - tig0021982:36589-36608 None:intergenic 30.0%
CTCTTCATCTTGGTGAAATT+CGG + tig0021982:34625-34644 Msa1396010:CDS 35.0%
GTTCATTGCCAATTCAAACA+TGG + tig0021982:34764-34783 Msa1396010:intron 35.0%
! CATCAATGGTTGATATCATG+CGG - tig0021982:34877-34896 None:intergenic 35.0%
GATCAACCATGTTGACATAT+CGG + tig0021982:34941-34960 Msa1396010:intron 35.0%
ATTGAAAGAACCCTTCGTTA+AGG - tig0021982:35011-35030 None:intergenic 35.0%
CTCATGACATCATTCTTAGA+AGG + tig0021982:35242-35261 Msa1396010:intron 35.0%
TTTAGTGTTTGCTTGATCGT+AGG - tig0021982:35377-35396 None:intergenic 35.0%
TCACTTGTGCACCATAAAAT+TGG - tig0021982:35424-35443 None:intergenic 35.0%
! AACTTGTTTGTTTCCACCAT+TGG + tig0021982:35467-35486 Msa1396010:intron 35.0%
GGCAATACTTATTGATCCAA+TGG - tig0021982:35486-35505 None:intergenic 35.0%
! GGATCAATAAGTATTGCCAT+TGG + tig0021982:35488-35507 Msa1396010:intron 35.0%
GTTGTGACTTATTGACCTAA+CGG + tig0021982:35525-35544 Msa1396010:intron 35.0%
!! CAAGTTTGTTTTCTACCGTT+AGG - tig0021982:35543-35562 None:intergenic 35.0%
GTAGAAAACAAACTTGTGCA+TGG + tig0021982:35547-35566 Msa1396010:intron 35.0%
GTTGCTATGCAAAATGAAGT+TGG - tig0021982:35633-35652 None:intergenic 35.0%
CCTTCAAACTCGGTTTAAAA+TGG - tig0021982:35669-35688 None:intergenic 35.0%
! CCATTTTAAACCGAGTTTGA+AGG + tig0021982:35666-35685 Msa1396010:intron 35.0%
CTAACTCTTGTTCAGTTGTT+TGG - tig0021982:35728-35747 None:intergenic 35.0%
GCTGTGTTAAAATAAGCTAC+GGG + tig0021982:35752-35771 Msa1396010:intron 35.0%
CTGTGTTAAAATAAGCTACG+GGG + tig0021982:35753-35772 Msa1396010:intron 35.0%
TAGTATGTACATGTGTGGTT+GGG - tig0021982:35793-35812 None:intergenic 35.0%
TTAGTATGTACATGTGTGGT+TGG - tig0021982:35794-35813 None:intergenic 35.0%
TGCTTTAGTGAACATAGATC+CGG + tig0021982:36163-36182 Msa1396010:CDS 35.0%
AACATTAAAGTCCGTACTTG+AGG - tig0021982:36240-36259 None:intergenic 35.0%
CAAGTACGGACTTTAATGTT+TGG + tig0021982:36240-36259 Msa1396010:CDS 35.0%
TTCATCAAGAGGAATGTGAT+GGG - tig0021982:36299-36318 None:intergenic 35.0%
TTTCATCAAGAGGAATGTGA+TGG - tig0021982:36300-36319 None:intergenic 35.0%
AAAGAAAGACGGAATGAAAG+GGG + tig0021982:36457-36476 Msa1396010:three_prime_UTR 35.0%
TGCCTTAGTCTATTAAAGGA+AGG + tig0021982:36490-36509 Msa1396010:three_prime_UTR 35.0%
AACGCAATGTCATCAACAAC+CGG + tig0021982:34252-34271 Msa1396010:CDS 40.0%
!! TTGATTTGGATTGGTCTTCG+CGG - tig0021982:34285-34304 None:intergenic 40.0%
!! GGTGGTATGTTGATTTGGAT+TGG - tig0021982:34294-34313 None:intergenic 40.0%
! AGGATGGTGGTATGTTGATT+TGG - tig0021982:34299-34318 None:intergenic 40.0%
GATGAGAAGAGCGATATGAA+CGG - tig0021982:34443-34462 None:intergenic 40.0%
!! TAGCTCTAATGTCGTCGTTT+GGG - tig0021982:34482-34501 None:intergenic 40.0%
!! TTAGCTCTAATGTCGTCGTT+TGG - tig0021982:34483-34502 None:intergenic 40.0%
TTATCCTTGGCAATAGGAGA+AGG - tig0021982:34531-34550 None:intergenic 40.0%
AATCCAACCACGATTATCCT+TGG - tig0021982:34544-34563 None:intergenic 40.0%
ACTTGTGCTTCTCTTCATCT+TGG + tig0021982:34615-34634 Msa1396010:CDS 40.0%
ATGTCAACATGGTTGATCGT+CGG - tig0021982:34939-34958 None:intergenic 40.0%
CAATGACCGATATGTCAACA+TGG - tig0021982:34950-34969 None:intergenic 40.0%
ATGAGCCAAATCCTTAACGA+AGG + tig0021982:34997-35016 Msa1396010:intron 40.0%
TGAGCCAAATCCTTAACGAA+GGG + tig0021982:34998-35017 Msa1396010:intron 40.0%
AGAACCCTTCGTTAAGGATT+TGG - tig0021982:35005-35024 None:intergenic 40.0%
TAAGGAGTTAAACATCCAGC+AGG - tig0021982:35184-35203 None:intergenic 40.0%
AGTTACAGGTAGCAGTTTAG+TGG - tig0021982:35277-35296 None:intergenic 40.0%
CTAAACTGCTACCTGTAACT+AGG + tig0021982:35277-35296 Msa1396010:intron 40.0%
! GAGCGTAATTTTCATCTTGC+TGG - tig0021982:35332-35351 None:intergenic 40.0%
GAAACAAACAAGTTCGTGCA+TGG - tig0021982:35461-35480 None:intergenic 40.0%
CACATCATTGCCTTCAAACT+CGG - tig0021982:35679-35698 None:intergenic 40.0%
!!! TCAGTTGTTTGGTTTTCGGT+TGG - tig0021982:35717-35736 None:intergenic 40.0%
GTTCACAGTAATCTGTTTGC+AGG + tig0021982:36046-36065 Msa1396010:intron 40.0%
AACAGCGAGGTAACTGATAT+TGG + tig0021982:36074-36093 Msa1396010:CDS 40.0%
! AACTGATATTGGCTATGCTG+AGG + tig0021982:36085-36104 Msa1396010:CDS 40.0%
TAGTGAACATAGATCCGGTT+CGG + tig0021982:36168-36187 Msa1396010:CDS 40.0%
ATAAAGTAAGCACTCCGAAC+CGG - tig0021982:36185-36204 None:intergenic 40.0%
! GTTCGGAGTGCTTACTTTAT+AGG + tig0021982:36185-36204 Msa1396010:CDS 40.0%
TCATCAAGAGGAATGTGATG+GGG - tig0021982:36298-36317 None:intergenic 40.0%
AAGAAAGACGGAATGAAAGG+GGG + tig0021982:36458-36477 Msa1396010:three_prime_UTR 40.0%
TTGAAGGAATGAGGTGACGA+GGG - tig0021982:34361-34380 None:intergenic 45.0%
TTTGAAGGAATGAGGTGACG+AGG - tig0021982:34362-34381 None:intergenic 45.0%
! GTGAGGTCTTTGAAGGAATG+AGG - tig0021982:34370-34389 None:intergenic 45.0%
! AGCTCTAATGTCGTCGTTTG+GGG - tig0021982:34481-34500 None:intergenic 45.0%
TGGCAATAGGAGAAGGAAAG+CGG - tig0021982:34524-34543 None:intergenic 45.0%
CTTTCCTTCTCCTATTGCCA+AGG + tig0021982:34524-34543 Msa1396010:CDS 45.0%
CCACGATTATCCTTGGCAAT+AGG - tig0021982:34537-34556 None:intergenic 45.0%
CCTATTGCCAAGGATAATCG+TGG + tig0021982:34534-34553 Msa1396010:CDS 45.0%
TTGCCAAGGATAATCGTGGT+TGG + tig0021982:34538-34557 Msa1396010:CDS 45.0%
! GAAGAAAGTGCGAGAGCAAT+AGG - tig0021982:34573-34592 None:intergenic 45.0%
GCACTTTCTTCCAGCATTTC+AGG + tig0021982:34582-34601 Msa1396010:CDS 45.0%
CTTTCTTCCAGCATTTCAGG+TGG + tig0021982:34585-34604 Msa1396010:CDS 45.0%
ATCTTGGTGAAATTCGGCCT+GGG + tig0021982:34631-34650 Msa1396010:CDS 45.0%
TGATTTCCTCTCAACTTCCC+AGG - tig0021982:34651-34670 None:intergenic 45.0%
!! GGGTGCTGCTATCAAGTTTA+GGG + tig0021982:34707-34726 Msa1396010:CDS 45.0%
!! GAAAAAGTGTTTGGTCCTGC+TGG + tig0021982:35166-35185 Msa1396010:intron 45.0%
CGAGTTTGAAGGCAATGATG+TGG + tig0021982:35677-35696 Msa1396010:intron 45.0%
ATAAGCTACGGGGTTCTCAA+CGG + tig0021982:35763-35782 Msa1396010:intron 45.0%
!! GATTAGCTTATGCGGTCTGT+AGG + tig0021982:35919-35938 Msa1396010:intron 45.0%
TGCGGTCTGTAGGCAAAATT+TGG + tig0021982:35929-35948 Msa1396010:intron 45.0%
GCGGTCTGTAGGCAAAATTT+GGG + tig0021982:35930-35949 Msa1396010:intron 45.0%
CACAGTAATCTGTTTGCAGG+TGG + tig0021982:36049-36068 Msa1396010:intron 45.0%
AACAACCTCATCTCTGCCAA+TGG - tig0021982:36114-36133 None:intergenic 45.0%
AAGCATGTGCAGTATGAGCA+GGG - tig0021982:36148-36167 None:intergenic 45.0%
AAAGCATGTGCAGTATGAGC+AGG - tig0021982:36149-36168 None:intergenic 45.0%
TGACATTGCGTTGCTGCTAC+TGG - tig0021982:34244-34263 None:intergenic 50.0%
! TTGAAACATGGCGGAAGTGG+AGG - tig0021982:34325-34344 None:intergenic 50.0%
CCACTTCCGCCATGTTTCAA+CGG + tig0021982:34325-34344 Msa1396010:CDS 50.0%
GGAGAGAGTGAGGTCTTTGA+AGG - tig0021982:34377-34396 None:intergenic 50.0%
GGAAGATGGAGAGGACGAAA+GGG - tig0021982:34398-34417 None:intergenic 50.0%
AGGAAGATGGAGAGGACGAA+AGG - tig0021982:34399-34418 None:intergenic 50.0%
GGCAATAGGAGAAGGAAAGC+GGG - tig0021982:34523-34542 None:intergenic 50.0%
AACAGCTCCACCTGAAATGC+TGG - tig0021982:34595-34614 None:intergenic 50.0%
CATCTTGGTGAAATTCGGCC+TGG + tig0021982:34630-34649 Msa1396010:CDS 50.0%
!! GGGGTGCTGCTATCAAGTTT+AGG + tig0021982:34706-34725 Msa1396010:CDS 50.0%
TGGGTCATGCGCACAAGTTA+AGG - tig0021982:35202-35221 None:intergenic 50.0%
TTTGCAGGTGGAAAACAGCG+AGG + tig0021982:36061-36080 Msa1396010:intron 50.0%
CATTGCGTTGCTGCTACTGG+TGG - tig0021982:34241-34260 None:intergenic 55.0%
! AAACATGGCGGAAGTGGAGG+AGG - tig0021982:34322-34341 None:intergenic 55.0%
! CCGTTGAAACATGGCGGAAG+TGG - tig0021982:34328-34347 None:intergenic 55.0%
GCGGATCCGTTGAAACATGG+CGG - tig0021982:34334-34353 None:intergenic 55.0%
AGGAATGAGGTGACGAGGGA+GGG - tig0021982:34357-34376 None:intergenic 55.0%
AAGGAATGAGGTGACGAGGG+AGG - tig0021982:34358-34377 None:intergenic 55.0%
AGGACGAAAGGGAGAGAGTG+AGG - tig0021982:34387-34406 None:intergenic 55.0%
CGAGGAAGAGGAAGATGGAG+AGG - tig0021982:34407-34426 None:intergenic 55.0%
CCAAGCGAGGAAGAGGAAGA+TGG - tig0021982:34412-34431 None:intergenic 55.0%
CCATCTTCCTCTTCCTCGCT+TGG + tig0021982:34409-34428 Msa1396010:CDS 55.0%
GAGAGATCCAAGCGAGGAAG+AGG - tig0021982:34419-34438 None:intergenic 55.0%
AACGGAGAGAGATCCAAGCG+AGG - tig0021982:34425-34444 None:intergenic 55.0%
TTTGGGGAAGAGAGAGGGAG+TGG - tig0021982:34465-34484 None:intergenic 55.0%
CGTCGTTTGGGGAAGAGAGA+GGG - tig0021982:34470-34489 None:intergenic 55.0%
TCGTCGTTTGGGGAAGAGAG+AGG - tig0021982:34471-34490 None:intergenic 55.0%
ATTCGGCCTGGGAAGTTGAG+AGG + tig0021982:34642-34661 Msa1396010:CDS 55.0%
GGCTATGCTGAGGTGACCAT+TGG + tig0021982:36095-36114 Msa1396010:CDS 55.0%
GGTGACCATTGGCAGAGATG+AGG + tig0021982:36106-36125 Msa1396010:CDS 55.0%
!! GTGGTGGTGGTGGTTCTCTC+TGG - tig0021982:34213-34232 None:intergenic 60.0%
!! GTTGCTGCTACTGGTGGTGG+TGG - tig0021982:34235-34254 None:intergenic 60.0%
TGCGTTGCTGCTACTGGTGG+TGG - tig0021982:34238-34257 None:intergenic 60.0%
GAAGTGGAGGAGGAGGATGG+TGG - tig0021982:34312-34331 None:intergenic 60.0%
GGGGCGGATCCGTTGAAACA+TGG - tig0021982:34337-34356 None:intergenic 60.0%
GGAATGAGGTGACGAGGGAG+GGG - tig0021982:34356-34375 None:intergenic 60.0%
!! ACTGGTGGTGGTGGTGGTGG+TGG - tig0021982:34226-34245 None:intergenic 65.0%
!! GCTACTGGTGGTGGTGGTGG+TGG - tig0021982:34229-34248 None:intergenic 65.0%
!! GCTGCTACTGGTGGTGGTGG+TGG - tig0021982:34232-34251 None:intergenic 65.0%
!! TGGTCTTCGCGGTGTTGCGC+CGG - tig0021982:34274-34293 None:intergenic 65.0%
GCGGAAGTGGAGGAGGAGGA+TGG - tig0021982:34315-34334 None:intergenic 65.0%
! CATGGCGGAAGTGGAGGAGG+AGG - tig0021982:34319-34338 None:intergenic 65.0%
ATGAGGTGACGAGGGAGGGG+CGG - tig0021982:34353-34372 None:intergenic 65.0%
!! GGTGGTGGTGGTGGTGGTGG+TGG - tig0021982:34220-34239 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
tig0021982 gene 34097 36621 34097 ID=Msa1396010;Name=Msa1396010
tig0021982 mRNA 34097 36621 34097 ID=Msa1396010-mRNA-1;Parent=Msa1396010;Name=Msa1396010-mRNA-1;_AED=0.01;_eAED=0.01;_QI=101|1|1|1|1|1|2|347|245
tig0021982 exon 34097 34728 34097 ID=Msa1396010-mRNA-1:exon:15214;Parent=Msa1396010-mRNA-1
tig0021982 exon 36068 36621 36068 ID=Msa1396010-mRNA-1:exon:15215;Parent=Msa1396010-mRNA-1
tig0021982 five_prime_UTR 34097 34197 34097 ID=Msa1396010-mRNA-1:five_prime_utr;Parent=Msa1396010-mRNA-1
tig0021982 CDS 34198 34728 34198 ID=Msa1396010-mRNA-1:cds;Parent=Msa1396010-mRNA-1
tig0021982 CDS 36068 36274 36068 ID=Msa1396010-mRNA-1:cds;Parent=Msa1396010-mRNA-1
tig0021982 three_prime_UTR 36275 36621 36275 ID=Msa1396010-mRNA-1:three_prime_utr;Parent=Msa1396010-mRNA-1
Gene Sequence

>Msa1396010

ATGAGAGTGCAACCAGAGAGAACCACCACCACCACCACCACCACCAGTAGCAGCAACGCAATGTCATCAACAACCGGCGCAACACCGCGAAGACCAATCCAAATCAACATACCACCATCCTCCTCCTCCACTTCCGCCATGTTTCAACGGATCCGCCCCTCCCTCGTCACCTCATTCCTTCAAAGACCTCACTCTCTCCCTTTCGTCCTCTCCATCTTCCTCTTCCTCGCTTGGATCTCTCTCCGTTCATATCGCTCTTCTCATCCACTCCCTCTCTCTTCCCCAAACGACGACATTAGAGCTAATCTTCGCAGATTCTCTTCCCGCTTTCCTTCTCCTATTGCCAAGGATAATCGTGGTTGGATTCTTGATCCTATTGCTCTCGCACTTTCTTCCAGCATTTCAGGTGGAGCTGTTACTTGTGCTTCTCTTCATCTTGGTGAAATTCGGCCTGGGAAGTTGAGAGGAAATCATAGACATCATGATTGTAATGAGACTTTTGTTCTTTGGGGTGCTGCTATCAAGTTTAGGGTGGAAAACAGCGAGGTAACTGATATTGGCTATGCTGAGGTGACCATTGGCAGAGATGAGGTTGTTGTTGCTGCTAGCCCTGCTCATACTGCACATGCTTTAGTGAACATAGATCCGGTTCGGAGTGCTTACTTTATAGGATGCCAAGATAGTATCATAAACTATAATGCCTCAAGTACGGACTTTAATGTTTGGAAAGATCTTTAA
Protein sequence

>Msa1396010

MRVQPERTTTTTTTTSSSNAMSSTTGATPRRPIQINIPPSSSSTSAMFQRIRPSLVTSFLQRPHSLPFVLSIFLFLAWISLRSYRSSHPLPLSSPNDDIRANLRRFSSRFPSPIAKDNRGWILDPIALALSSSISGGAVTCASLHLGEIRPGKLRGNHRHHDCNETFVLWGAAIKFRVENSEVTDIGYAEVTIGRDEVVVAASPAHTAHALVNIDPVRSAYFIGCQDSIINYNASSTDFNVWKDL*