AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1413430

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1413430 MtrunA17_Chr4g0076821 86.253 371 44 4 1 370 1 365 0.0 533
Msa1413430 MtrunA17_Chr1g0188441 42.500 240 82 5 114 353 5 188 5.86e-61 194
Msa1413430 MtrunA17_Chr5g0404271 41.633 245 102 7 111 353 92 297 2.82e-53 178
Msa1413430 MtrunA17_Chr3g0133181 40.000 245 107 5 109 353 71 275 2.74e-50 170
Msa1413430 MtrunA17_Chr5g0427051 41.057 246 101 7 116 351 56 267 5.25e-50 169
Msa1413430 MtrunA17_Chr2g0315941 37.959 245 112 8 110 351 60 267 1.81e-42 149
Msa1413430 MtrunA17_Chr7g0217231 70.312 64 19 0 289 352 220 283 2.84e-26 107
Msa1413430 MtrunA17_Chr6g0453231 64.286 70 24 1 289 358 222 290 1.28e-24 102
Msa1413430 MtrunA17_Chr6g0453231 57.692 52 20 1 118 167 91 142 1.83e-13 70.9
Msa1413430 MtrunA17_Chr5g0412501 74.510 51 13 0 117 167 77 127 1.16e-22 96.3
Msa1413430 MtrunA17_Chr8g0361671 58.824 68 28 0 289 356 271 338 1.93e-22 97.4
Msa1413430 MtrunA17_Chr3g0092371 56.757 74 30 1 101 172 1 74 3.07e-20 84.3
Msa1413430 MtrunA17_Chr8g0367691 53.731 67 29 1 103 167 52 118 1.94e-19 87.0
Msa1413430 MtrunA17_Chr6g0479901 55.000 60 26 1 289 347 101 160 7.90e-19 83.2
Msa1413430 MtrunA17_Chr6g0479901 60.000 50 19 1 118 166 19 68 1.29e-14 71.6
Msa1413430 MtrunA17_Chr8g0357741 59.677 62 24 1 290 351 36 96 1.68e-16 74.3
Msa1413430 MtrunA17_Chr6g0453221 61.538 52 16 1 115 166 18 65 3.97e-14 67.4
Msa1413430 MtrunA17_Chr4g0060731 56.250 64 23 2 111 173 18 77 4.31e-14 67.0
Msa1413430 MtrunA17_Chr7g0243651 56.098 41 18 0 116 156 1 41 7.61e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1413430 AT2G02540.2 47.581 248 90 4 106 351 75 284 7.86e-69 219
Msa1413430 AT2G02540.1 47.581 248 90 4 106 351 75 284 7.86e-69 219
Msa1413430 AT1G14440.1 48.571 245 67 7 115 351 87 280 5.61e-62 201
Msa1413430 AT1G14440.2 48.571 245 67 7 115 351 87 280 5.61e-62 201
Msa1413430 AT2G18350.1 37.234 282 101 7 84 353 45 262 7.30e-48 163
Msa1413430 AT1G69600.1 37.143 245 96 7 116 354 29 221 3.88e-43 150
Msa1413430 AT5G60480.1 31.489 235 96 5 114 346 33 204 7.37e-34 125
Msa1413430 AT1G75240.1 78.571 56 12 0 115 170 73 128 3.24e-26 107
Msa1413430 AT4G24660.1 64.516 62 22 0 290 351 158 219 7.85e-24 98.6
Msa1413430 AT4G24660.1 72.222 54 15 0 116 169 47 100 8.47e-23 95.9
Msa1413430 AT4G24660.2 56.164 73 32 0 290 362 158 230 4.60e-23 96.7
Msa1413430 AT4G24660.2 72.222 54 15 0 116 169 47 100 8.68e-23 95.9
Msa1413430 AT5G65410.1 60.317 63 25 0 288 350 190 252 9.88e-23 97.1
Msa1413430 AT5G65410.1 69.811 53 16 0 116 168 73 125 5.52e-21 92.0
Msa1413430 AT5G39760.1 51.220 82 38 1 289 368 200 281 2.92e-21 94.0
Msa1413430 AT5G15210.1 63.158 57 21 0 289 345 179 235 3.58e-21 92.4
Msa1413430 AT5G15210.1 61.818 55 19 1 115 167 53 107 6.91e-16 77.4
Msa1413430 AT1G74660.1 66.667 57 19 0 116 172 37 93 5.73e-21 87.0
Msa1413430 AT1G74660.2 67.857 56 18 0 116 171 37 92 7.81e-21 86.7
Msa1413430 AT3G28917.1 66.667 57 18 1 116 171 31 87 2.14e-20 85.5
Msa1413430 AT3G28920.1 60.000 60 24 0 289 348 192 251 2.79e-20 90.5
Msa1413430 AT3G50890.1 65.455 55 19 0 116 170 58 112 7.49e-20 88.2
Msa1413430 AT3G50890.1 47.368 57 30 0 294 350 186 242 2.09e-14 72.8
Msa1413430 AT1G18835.1 63.158 57 18 1 116 172 24 77 8.50e-17 75.1
Msa1413430 AT5G42780.1 60.784 51 18 1 118 168 64 112 4.38e-13 68.9
Msa1413430 AT5G42780.1 41.176 68 38 1 281 348 173 238 1.05e-12 67.8

Find 84 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCCATTAACTGAAGGGTTT+TGG 0.183475 tig0024026:+78596 None:intergenic
TTATTATTATTGTGGTTTAT+TGG 0.190415 tig0024026:+78796 None:intergenic
TATTATTATTGTGGTTTATT+GGG 0.222211 tig0024026:+78797 None:intergenic
CCCCAAAACCCTTCAGTTAA+TGG 0.254181 tig0024026:-78598 Msa1413430:CDS
CCTGATCAGGCTCTTGGTCT+TGG 0.311106 tig0024026:-78220 Msa1413430:CDS
CTCTTCTTGTTCATCAGATT+CGG 0.338219 tig0024026:+78057 None:intergenic
AATGGTTCCTTGTTCACCAC+TGG 0.354032 tig0024026:+78405 None:intergenic
TTATTGGGATTTCTCCATCT+TGG 0.359976 tig0024026:+78812 None:intergenic
GTTGCTGCTGATGCTGTATT+AGG 0.369444 tig0024026:+78175 None:intergenic
CCTCTTCCTGATCAGGCTCT+TGG 0.375629 tig0024026:-78226 Msa1413430:CDS
CATGTTACCTCTTCCTGATC+AGG 0.387160 tig0024026:-78233 Msa1413430:CDS
CCTTCAACTTCAACTTCAAC+AGG 0.390687 tig0024026:-78282 Msa1413430:CDS
CCTGTTGAAGTTGAAGTTGA+AGG 0.398274 tig0024026:+78282 None:intergenic
GGTACTACTGCCACTGGTAC+TGG 0.421288 tig0024026:+78619 None:intergenic
TGGTGTAGTGGAGGAGGATG+AGG 0.421641 tig0024026:+78688 None:intergenic
GATTTCTCCATCTTGGCTGT+TGG 0.421739 tig0024026:+78819 None:intergenic
AGAGCAGTTGAGTGCTTCAA+TGG 0.440838 tig0024026:+78387 None:intergenic
AATAATTATGGTACTAGTAA+TGG 0.445222 tig0024026:-78754 Msa1413430:CDS
AAAGAAGAGTGCTCAAGGTT+TGG 0.446033 tig0024026:-77832 Msa1413430:CDS
GAAGGTATGTTGTAGTTGAA+TGG 0.452483 tig0024026:+78079 None:intergenic
CCTGATCCTCAGTCTCACCT+TGG 0.455452 tig0024026:+78326 None:intergenic
CCAAGACCAAGAGCCTGATC+AGG 0.460517 tig0024026:+78220 None:intergenic
CCAAGGTGAGACTGAGGATC+AGG 0.460530 tig0024026:-78326 Msa1413430:CDS
AACTGATGACGATTTCTTGT+AGG 0.461489 tig0024026:+78510 None:intergenic
AGCAGGTAGTGATGAAGAAA+AGG 0.463951 tig0024026:-77979 Msa1413430:CDS
TAGTGATGAAGAAAAGGTTC+AGG 0.467781 tig0024026:-77973 Msa1413430:CDS
CAACAGTTCTGCCAAGAGAT+TGG 0.479137 tig0024026:-77860 Msa1413430:CDS
TAGGAGTAGAGTGGTGGTGT+TGG 0.483759 tig0024026:+78194 None:intergenic
AATACAGCATCAGCAGCAAC+AGG 0.487107 tig0024026:-78172 Msa1413430:CDS
AAGAATCATGCAGCTGCAAT+GGG 0.493612 tig0024026:-78463 Msa1413430:CDS
ATGGTTCCTTGTTCACCACT+GGG 0.498200 tig0024026:+78406 None:intergenic
AAAGAATCATGCAGCTGCAA+TGG 0.500219 tig0024026:-78464 Msa1413430:CDS
CGCAGTAGTGCCAGTACCAG+TGG 0.506274 tig0024026:-78629 Msa1413430:CDS
GGCAGCACCACCACAGCATG+AGG 0.512800 tig0024026:-78261 Msa1413430:CDS
GAACTTTCCAACAGCCAAGA+TGG 0.514451 tig0024026:-78826 Msa1413430:CDS
CAAGCAGCAGCTGTTAGTAC+TGG 0.515000 tig0024026:-78559 Msa1413430:CDS
AGAGCTGTAGCGCCACCTCA+TGG 0.521678 tig0024026:-78121 Msa1413430:CDS
GGTGTAGTGGAGGAGGATGA+GGG 0.535345 tig0024026:+78689 None:intergenic
GGTGGCGCTACAGCTCTAGA+AGG 0.538474 tig0024026:+78127 None:intergenic
CGAATCTGATGAACAAGAAG+AGG 0.550515 tig0024026:-78056 Msa1413430:CDS
ATATAATATTACTGTGATTG+TGG 0.551597 tig0024026:+78668 None:intergenic
GAAGTTTCTGTGACAATGGC+AGG 0.555632 tig0024026:+78363 None:intergenic
CCAAGAGCCTGATCAGGAAG+AGG 0.556475 tig0024026:+78226 None:intergenic
TCTTGTTCATCAGATTCGGA+AGG 0.560538 tig0024026:+78061 None:intergenic
CTAACAGCTGCTGCTTGCAT+AGG 0.566961 tig0024026:+78565 None:intergenic
AAAATTCACACAGGAACAGA+AGG 0.568057 tig0024026:-77948 Msa1413430:CDS
GTGGTTGTGCCATTAACTGA+AGG 0.568135 tig0024026:+78589 None:intergenic
TGATTGTGGTGTAGTGGAGG+AGG 0.569506 tig0024026:+78682 None:intergenic
TTCATGCCCAGTGGTGAACA+AGG 0.570431 tig0024026:-78412 Msa1413430:CDS
TGTGGAAGTTTCTGTGACAA+TGG 0.570726 tig0024026:+78359 None:intergenic
CAAGAAATCGTCATCAGTTG+TGG 0.580092 tig0024026:-78506 Msa1413430:CDS
GATGAACAAGAAGAGGCAGC+AGG 0.580135 tig0024026:-78049 Msa1413430:CDS
TGGTTGTGCCATTAACTGAA+GGG 0.581647 tig0024026:+78590 None:intergenic
TGATGACGATTTCTTGTAGG+AGG 0.584121 tig0024026:+78513 None:intergenic
CTGTGATTGTGGTGTAGTGG+AGG 0.591651 tig0024026:+78679 None:intergenic
AATCATGCAGCTGCAATGGG+AGG 0.592565 tig0024026:-78460 Msa1413430:CDS
GTAGCGCCACCTCATGGACA+TGG 0.593213 tig0024026:-78115 Msa1413430:CDS
TGAATTTCCTCATGCTGTGG+TGG 0.602127 tig0024026:+78254 None:intergenic
TGTGAAAAGAAGAGTGCTCA+AGG 0.602235 tig0024026:-77837 Msa1413430:CDS
ATCATGCAGCTGCAATGGGA+GGG 0.602761 tig0024026:-78459 Msa1413430:CDS
ATGGAAATTTCAGAAGCAAG+AGG 0.613533 tig0024026:-77894 Msa1413430:CDS
GGGAATGCCACAGATGGCTG+TGG 0.614228 tig0024026:-78439 Msa1413430:CDS
TTACTGTGATTGTGGTGTAG+TGG 0.616570 tig0024026:+78676 None:intergenic
TGAAGTTGAAGGCTGAGTGA+TGG 0.622721 tig0024026:+78293 None:intergenic
AGTTGAAGGCTGAGTGATGG+TGG 0.623553 tig0024026:+78296 None:intergenic
ATGGGAGGGAATGCCACAGA+TGG 0.625053 tig0024026:-78445 Msa1413430:CDS
AGCTGCTGCTTGCATAGGAG+TGG 0.626710 tig0024026:+78570 None:intergenic
AGAAGTCCAAGGTGAGACTG+AGG 0.639644 tig0024026:-78332 Msa1413430:CDS
GGTGTTCATGAACATGATGA+TGG 0.640624 tig0024026:+78710 None:intergenic
AGCAGCTGTTAGTACTGGTG+TGG 0.641970 tig0024026:-78554 Msa1413430:CDS
CACTGGTACTGGCACTACTG+CGG 0.649774 tig0024026:+78630 None:intergenic
TTCCACAGAAAAGAAGTCCA+AGG 0.650236 tig0024026:-78343 Msa1413430:CDS
TCAGAAGCAAGAGGAATCTG+TGG 0.650938 tig0024026:-77885 Msa1413430:CDS
AGTTGAATGGCATGATGATG+TGG 0.657862 tig0024026:+78092 None:intergenic
ATGTGGCCATGTCCATGAGG+TGG 0.659218 tig0024026:+78109 None:intergenic
ACATGAATTTCCTCATGCTG+TGG 0.665457 tig0024026:+78251 None:intergenic
AATCGTCATCAGTTGTGGTG+AGG 0.674142 tig0024026:-78501 Msa1413430:CDS
CTGTATTAGGAGTAGAGTGG+TGG 0.678813 tig0024026:+78188 None:intergenic
TGTGGTGAGTTCATGCCCAG+TGG 0.682320 tig0024026:-78421 Msa1413430:CDS
TATGGTACTAGTAATGGACA+TGG 0.682582 tig0024026:-78748 Msa1413430:CDS
GAACTCACCACAGCCATCTG+TGG 0.688684 tig0024026:+78432 None:intergenic
GTTCAGGACAAAATTCACAC+AGG 0.704613 tig0024026:-77957 Msa1413430:CDS
ATGCTGTATTAGGAGTAGAG+TGG 0.716681 tig0024026:+78185 None:intergenic
ATGATGTGGCCATGTCCATG+AGG 0.730801 tig0024026:+78106 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATAATAATAATAATTA+TGG - tig0024026:77802-77821 Msa1413430:CDS 0.0%
!! AATAATAATAATAATAATTA+TGG - tig0024026:77802-77821 Msa1413430:CDS 0.0%
!! TATTATTATTGTGGTTTATT+GGG + tig0024026:77774-77793 None:intergenic 15.0%
!! TTATTATTATTGTGGTTTAT+TGG + tig0024026:77775-77794 None:intergenic 15.0%
!! TATTATTATTGTGGTTTATT+GGG + tig0024026:77774-77793 None:intergenic 15.0%
!! TTATTATTATTGTGGTTTAT+TGG + tig0024026:77775-77794 None:intergenic 15.0%
!! AATAATTATGGTACTAGTAA+TGG - tig0024026:77814-77833 Msa1413430:CDS 20.0%
!! ATATAATATTACTGTGATTG+TGG + tig0024026:77903-77922 None:intergenic 20.0%
!! AATAATTATGGTACTAGTAA+TGG - tig0024026:77814-77833 Msa1413430:CDS 20.0%
!! ATATAATATTACTGTGATTG+TGG + tig0024026:77903-77922 None:intergenic 20.0%
!!! TTCATGTTGAAGTTGTTTTT+TGG + tig0024026:78787-78806 None:intergenic 25.0%
!!! TTCATGTTGAAGTTGTTTTT+TGG + tig0024026:78787-78806 None:intergenic 25.0%
! TTCAATTTTGCTGAGAAAGT+TGG - tig0024026:78651-78670 Msa1413430:CDS 30.0%
! TTCAATTTTGCTGAGAAAGT+TGG - tig0024026:78651-78670 Msa1413430:CDS 30.0%
TTATTGGGATTTCTCCATCT+TGG + tig0024026:77759-77778 None:intergenic 35.0%
!! TATGGTACTAGTAATGGACA+TGG - tig0024026:77820-77839 Msa1413430:CDS 35.0%
! AACTGATGACGATTTCTTGT+AGG + tig0024026:78061-78080 None:intergenic 35.0%
! GAAGGTATGTTGTAGTTGAA+TGG + tig0024026:78492-78511 None:intergenic 35.0%
CTCTTCTTGTTCATCAGATT+CGG + tig0024026:78514-78533 None:intergenic 35.0%
TAGTGATGAAGAAAAGGTTC+AGG - tig0024026:78595-78614 Msa1413430:CDS 35.0%
AAAATTCACACAGGAACAGA+AGG - tig0024026:78620-78639 Msa1413430:CDS 35.0%
! ATTTTGCTGAGAAAGTTGGA+TGG - tig0024026:78655-78674 Msa1413430:CDS 35.0%
ATGGAAATTTCAGAAGCAAG+AGG - tig0024026:78674-78693 Msa1413430:CDS 35.0%
! TTCTTTTCACACCAATCTCT+TGG + tig0024026:78722-78741 None:intergenic 35.0%
TTATTGGGATTTCTCCATCT+TGG + tig0024026:77759-77778 None:intergenic 35.0%
!! TATGGTACTAGTAATGGACA+TGG - tig0024026:77820-77839 Msa1413430:CDS 35.0%
! AACTGATGACGATTTCTTGT+AGG + tig0024026:78061-78080 None:intergenic 35.0%
! GAAGGTATGTTGTAGTTGAA+TGG + tig0024026:78492-78511 None:intergenic 35.0%
CTCTTCTTGTTCATCAGATT+CGG + tig0024026:78514-78533 None:intergenic 35.0%
TAGTGATGAAGAAAAGGTTC+AGG - tig0024026:78595-78614 Msa1413430:CDS 35.0%
AAAATTCACACAGGAACAGA+AGG - tig0024026:78620-78639 Msa1413430:CDS 35.0%
! ATTTTGCTGAGAAAGTTGGA+TGG - tig0024026:78655-78674 Msa1413430:CDS 35.0%
ATGGAAATTTCAGAAGCAAG+AGG - tig0024026:78674-78693 Msa1413430:CDS 35.0%
! TTCTTTTCACACCAATCTCT+TGG + tig0024026:78722-78741 None:intergenic 35.0%
GGTGTTCATGAACATGATGA+TGG + tig0024026:77861-77880 None:intergenic 40.0%
TTACTGTGATTGTGGTGTAG+TGG + tig0024026:77895-77914 None:intergenic 40.0%
! CCATTAACTGAAGGGTTTTG+GGG + tig0024026:77973-77992 None:intergenic 40.0%
! GCCATTAACTGAAGGGTTTT+GGG + tig0024026:77974-77993 None:intergenic 40.0%
! TGCCATTAACTGAAGGGTTT+TGG + tig0024026:77975-77994 None:intergenic 40.0%
! TGGTTGTGCCATTAACTGAA+GGG + tig0024026:77981-78000 None:intergenic 40.0%
! TGATGACGATTTCTTGTAGG+AGG + tig0024026:78058-78077 None:intergenic 40.0%
CAAGAAATCGTCATCAGTTG+TGG - tig0024026:78062-78081 Msa1413430:CDS 40.0%
AAAGAATCATGCAGCTGCAA+TGG - tig0024026:78104-78123 Msa1413430:CDS 40.0%
AAGAATCATGCAGCTGCAAT+GGG - tig0024026:78105-78124 Msa1413430:CDS 40.0%
TGTGGAAGTTTCTGTGACAA+TGG + tig0024026:78212-78231 None:intergenic 40.0%
TTCCACAGAAAAGAAGTCCA+AGG - tig0024026:78225-78244 Msa1413430:CDS 40.0%
CCTGTTGAAGTTGAAGTTGA+AGG + tig0024026:78289-78308 None:intergenic 40.0%
CCTTCAACTTCAACTTCAAC+AGG - tig0024026:78286-78305 Msa1413430:CDS 40.0%
ACATGAATTTCCTCATGCTG+TGG + tig0024026:78320-78339 None:intergenic 40.0%
ATGCTGTATTAGGAGTAGAG+TGG + tig0024026:78386-78405 None:intergenic 40.0%
! AGTTGAATGGCATGATGATG+TGG + tig0024026:78479-78498 None:intergenic 40.0%
TCTTGTTCATCAGATTCGGA+AGG + tig0024026:78510-78529 None:intergenic 40.0%
CGAATCTGATGAACAAGAAG+AGG - tig0024026:78512-78531 Msa1413430:CDS 40.0%
AGCAGGTAGTGATGAAGAAA+AGG - tig0024026:78589-78608 Msa1413430:CDS 40.0%
GTTCAGGACAAAATTCACAC+AGG - tig0024026:78611-78630 Msa1413430:CDS 40.0%
!! TGTGAAAAGAAGAGTGCTCA+AGG - tig0024026:78731-78750 Msa1413430:CDS 40.0%
!! AAAGAAGAGTGCTCAAGGTT+TGG - tig0024026:78736-78755 Msa1413430:CDS 40.0%
GGTGTTCATGAACATGATGA+TGG + tig0024026:77861-77880 None:intergenic 40.0%
TTACTGTGATTGTGGTGTAG+TGG + tig0024026:77895-77914 None:intergenic 40.0%
! CCATTAACTGAAGGGTTTTG+GGG + tig0024026:77973-77992 None:intergenic 40.0%
! GCCATTAACTGAAGGGTTTT+GGG + tig0024026:77974-77993 None:intergenic 40.0%
! TGCCATTAACTGAAGGGTTT+TGG + tig0024026:77975-77994 None:intergenic 40.0%
! TGGTTGTGCCATTAACTGAA+GGG + tig0024026:77981-78000 None:intergenic 40.0%
! TGATGACGATTTCTTGTAGG+AGG + tig0024026:78058-78077 None:intergenic 40.0%
CAAGAAATCGTCATCAGTTG+TGG - tig0024026:78062-78081 Msa1413430:CDS 40.0%
AAAGAATCATGCAGCTGCAA+TGG - tig0024026:78104-78123 Msa1413430:CDS 40.0%
AAGAATCATGCAGCTGCAAT+GGG - tig0024026:78105-78124 Msa1413430:CDS 40.0%
TGTGGAAGTTTCTGTGACAA+TGG + tig0024026:78212-78231 None:intergenic 40.0%
TTCCACAGAAAAGAAGTCCA+AGG - tig0024026:78225-78244 Msa1413430:CDS 40.0%
CCTGTTGAAGTTGAAGTTGA+AGG + tig0024026:78289-78308 None:intergenic 40.0%
CCTTCAACTTCAACTTCAAC+AGG - tig0024026:78286-78305 Msa1413430:CDS 40.0%
ACATGAATTTCCTCATGCTG+TGG + tig0024026:78320-78339 None:intergenic 40.0%
ATGCTGTATTAGGAGTAGAG+TGG + tig0024026:78386-78405 None:intergenic 40.0%
! AGTTGAATGGCATGATGATG+TGG + tig0024026:78479-78498 None:intergenic 40.0%
TCTTGTTCATCAGATTCGGA+AGG + tig0024026:78510-78529 None:intergenic 40.0%
CGAATCTGATGAACAAGAAG+AGG - tig0024026:78512-78531 Msa1413430:CDS 40.0%
AGCAGGTAGTGATGAAGAAA+AGG - tig0024026:78589-78608 Msa1413430:CDS 40.0%
GTTCAGGACAAAATTCACAC+AGG - tig0024026:78611-78630 Msa1413430:CDS 40.0%
!! TGTGAAAAGAAGAGTGCTCA+AGG - tig0024026:78731-78750 Msa1413430:CDS 40.0%
!! AAAGAAGAGTGCTCAAGGTT+TGG - tig0024026:78736-78755 Msa1413430:CDS 40.0%
GAACTTTCCAACAGCCAAGA+TGG - tig0024026:77742-77761 Msa1413430:CDS 45.0%
GATTTCTCCATCTTGGCTGT+TGG + tig0024026:77752-77771 None:intergenic 45.0%
CCCCAAAACCCTTCAGTTAA+TGG - tig0024026:77970-77989 Msa1413430:CDS 45.0%
! GTGGTTGTGCCATTAACTGA+AGG + tig0024026:77982-78001 None:intergenic 45.0%
AATCGTCATCAGTTGTGGTG+AGG - tig0024026:78067-78086 Msa1413430:CDS 45.0%
ATGGTTCCTTGTTCACCACT+GGG + tig0024026:78165-78184 None:intergenic 45.0%
AATGGTTCCTTGTTCACCAC+TGG + tig0024026:78166-78185 None:intergenic 45.0%
AGAGCAGTTGAGTGCTTCAA+TGG + tig0024026:78184-78203 None:intergenic 45.0%
GAAGTTTCTGTGACAATGGC+AGG + tig0024026:78208-78227 None:intergenic 45.0%
! CACCTTGGACTTCTTTTCTG+TGG + tig0024026:78230-78249 None:intergenic 45.0%
TGAAGTTGAAGGCTGAGTGA+TGG + tig0024026:78278-78297 None:intergenic 45.0%
TGAATTTCCTCATGCTGTGG+TGG + tig0024026:78317-78336 None:intergenic 45.0%
CATGTTACCTCTTCCTGATC+AGG - tig0024026:78335-78354 Msa1413430:CDS 45.0%
CTGTATTAGGAGTAGAGTGG+TGG + tig0024026:78383-78402 None:intergenic 45.0%
GTTGCTGCTGATGCTGTATT+AGG + tig0024026:78396-78415 None:intergenic 45.0%
AATACAGCATCAGCAGCAAC+AGG - tig0024026:78396-78415 Msa1413430:CDS 45.0%
TCAGAAGCAAGAGGAATCTG+TGG - tig0024026:78683-78702 Msa1413430:CDS 45.0%
CAACAGTTCTGCCAAGAGAT+TGG - tig0024026:78708-78727 Msa1413430:CDS 45.0%
GAACTTTCCAACAGCCAAGA+TGG - tig0024026:77742-77761 Msa1413430:CDS 45.0%
GATTTCTCCATCTTGGCTGT+TGG + tig0024026:77752-77771 None:intergenic 45.0%
CCCCAAAACCCTTCAGTTAA+TGG - tig0024026:77970-77989 Msa1413430:CDS 45.0%
! GTGGTTGTGCCATTAACTGA+AGG + tig0024026:77982-78001 None:intergenic 45.0%
AATCGTCATCAGTTGTGGTG+AGG - tig0024026:78067-78086 Msa1413430:CDS 45.0%
ATGGTTCCTTGTTCACCACT+GGG + tig0024026:78165-78184 None:intergenic 45.0%
AATGGTTCCTTGTTCACCAC+TGG + tig0024026:78166-78185 None:intergenic 45.0%
AGAGCAGTTGAGTGCTTCAA+TGG + tig0024026:78184-78203 None:intergenic 45.0%
GAAGTTTCTGTGACAATGGC+AGG + tig0024026:78208-78227 None:intergenic 45.0%
! CACCTTGGACTTCTTTTCTG+TGG + tig0024026:78230-78249 None:intergenic 45.0%
TGAAGTTGAAGGCTGAGTGA+TGG + tig0024026:78278-78297 None:intergenic 45.0%
TGAATTTCCTCATGCTGTGG+TGG + tig0024026:78317-78336 None:intergenic 45.0%
CATGTTACCTCTTCCTGATC+AGG - tig0024026:78335-78354 Msa1413430:CDS 45.0%
CTGTATTAGGAGTAGAGTGG+TGG + tig0024026:78383-78402 None:intergenic 45.0%
GTTGCTGCTGATGCTGTATT+AGG + tig0024026:78396-78415 None:intergenic 45.0%
AATACAGCATCAGCAGCAAC+AGG - tig0024026:78396-78415 Msa1413430:CDS 45.0%
TCAGAAGCAAGAGGAATCTG+TGG - tig0024026:78683-78702 Msa1413430:CDS 45.0%
CAACAGTTCTGCCAAGAGAT+TGG - tig0024026:78708-78727 Msa1413430:CDS 45.0%
!! TATTATTATTATTATTATTG+TGG + tig0024026:77783-77802 None:intergenic 5.0%
!! TATTATTATTATTATTATTG+TGG + tig0024026:77783-77802 None:intergenic 5.0%
! TGATTGTGGTGTAGTGGAGG+AGG + tig0024026:77889-77908 None:intergenic 50.0%
CTGTGATTGTGGTGTAGTGG+AGG + tig0024026:77892-77911 None:intergenic 50.0%
!!! TTTTGGGGTACTACTGCCAC+TGG + tig0024026:77958-77977 None:intergenic 50.0%
! CTAACAGCTGCTGCTTGCAT+AGG + tig0024026:78006-78025 None:intergenic 50.0%
CAAGCAGCAGCTGTTAGTAC+TGG - tig0024026:78009-78028 Msa1413430:CDS 50.0%
!! AGCAGCTGTTAGTACTGGTG+TGG - tig0024026:78014-78033 Msa1413430:CDS 50.0%
AATCATGCAGCTGCAATGGG+AGG - tig0024026:78108-78127 Msa1413430:CDS 50.0%
ATCATGCAGCTGCAATGGGA+GGG - tig0024026:78109-78128 Msa1413430:CDS 50.0%
TTCATGCCCAGTGGTGAACA+AGG - tig0024026:78156-78175 Msa1413430:CDS 50.0%
AGAAGTCCAAGGTGAGACTG+AGG - tig0024026:78236-78255 Msa1413430:CDS 50.0%
AGTTGAAGGCTGAGTGATGG+TGG + tig0024026:78275-78294 None:intergenic 50.0%
! TAGGAGTAGAGTGGTGGTGT+TGG + tig0024026:78377-78396 None:intergenic 50.0%
ATGATGTGGCCATGTCCATG+AGG + tig0024026:78465-78484 None:intergenic 50.0%
GATGAACAAGAAGAGGCAGC+AGG - tig0024026:78519-78538 Msa1413430:CDS 50.0%
! TGATTGTGGTGTAGTGGAGG+AGG + tig0024026:77889-77908 None:intergenic 50.0%
CTGTGATTGTGGTGTAGTGG+AGG + tig0024026:77892-77911 None:intergenic 50.0%
!!! TTTTGGGGTACTACTGCCAC+TGG + tig0024026:77958-77977 None:intergenic 50.0%
! CTAACAGCTGCTGCTTGCAT+AGG + tig0024026:78006-78025 None:intergenic 50.0%
CAAGCAGCAGCTGTTAGTAC+TGG - tig0024026:78009-78028 Msa1413430:CDS 50.0%
!! AGCAGCTGTTAGTACTGGTG+TGG - tig0024026:78014-78033 Msa1413430:CDS 50.0%
AATCATGCAGCTGCAATGGG+AGG - tig0024026:78108-78127 Msa1413430:CDS 50.0%
ATCATGCAGCTGCAATGGGA+GGG - tig0024026:78109-78128 Msa1413430:CDS 50.0%
TTCATGCCCAGTGGTGAACA+AGG - tig0024026:78156-78175 Msa1413430:CDS 50.0%
AGAAGTCCAAGGTGAGACTG+AGG - tig0024026:78236-78255 Msa1413430:CDS 50.0%
AGTTGAAGGCTGAGTGATGG+TGG + tig0024026:78275-78294 None:intergenic 50.0%
! TAGGAGTAGAGTGGTGGTGT+TGG + tig0024026:78377-78396 None:intergenic 50.0%
ATGATGTGGCCATGTCCATG+AGG + tig0024026:78465-78484 None:intergenic 50.0%
GATGAACAAGAAGAGGCAGC+AGG - tig0024026:78519-78538 Msa1413430:CDS 50.0%
! GGTGTAGTGGAGGAGGATGA+GGG + tig0024026:77882-77901 None:intergenic 55.0%
! TGGTGTAGTGGAGGAGGATG+AGG + tig0024026:77883-77902 None:intergenic 55.0%
!! CACTGGTACTGGCACTACTG+CGG + tig0024026:77941-77960 None:intergenic 55.0%
GGTACTACTGCCACTGGTAC+TGG + tig0024026:77952-77971 None:intergenic 55.0%
! AGCTGCTGCTTGCATAGGAG+TGG + tig0024026:78001-78020 None:intergenic 55.0%
ATGGGAGGGAATGCCACAGA+TGG - tig0024026:78123-78142 Msa1413430:CDS 55.0%
GAACTCACCACAGCCATCTG+TGG + tig0024026:78139-78158 None:intergenic 55.0%
TGTGGTGAGTTCATGCCCAG+TGG - tig0024026:78147-78166 Msa1413430:CDS 55.0%
CCTGATCCTCAGTCTCACCT+TGG + tig0024026:78245-78264 None:intergenic 55.0%
CCAAGGTGAGACTGAGGATC+AGG - tig0024026:78242-78261 Msa1413430:CDS 55.0%
CCAAGAGCCTGATCAGGAAG+AGG + tig0024026:78345-78364 None:intergenic 55.0%
CCTCTTCCTGATCAGGCTCT+TGG - tig0024026:78342-78361 Msa1413430:CDS 55.0%
CCAAGACCAAGAGCCTGATC+AGG + tig0024026:78351-78370 None:intergenic 55.0%
CCTGATCAGGCTCTTGGTCT+TGG - tig0024026:78348-78367 Msa1413430:CDS 55.0%
ATGTGGCCATGTCCATGAGG+TGG + tig0024026:78462-78481 None:intergenic 55.0%
! GGTGTAGTGGAGGAGGATGA+GGG + tig0024026:77882-77901 None:intergenic 55.0%
! TGGTGTAGTGGAGGAGGATG+AGG + tig0024026:77883-77902 None:intergenic 55.0%
!! CACTGGTACTGGCACTACTG+CGG + tig0024026:77941-77960 None:intergenic 55.0%
GGTACTACTGCCACTGGTAC+TGG + tig0024026:77952-77971 None:intergenic 55.0%
! AGCTGCTGCTTGCATAGGAG+TGG + tig0024026:78001-78020 None:intergenic 55.0%
ATGGGAGGGAATGCCACAGA+TGG - tig0024026:78123-78142 Msa1413430:CDS 55.0%
GAACTCACCACAGCCATCTG+TGG + tig0024026:78139-78158 None:intergenic 55.0%
TGTGGTGAGTTCATGCCCAG+TGG - tig0024026:78147-78166 Msa1413430:CDS 55.0%
CCTGATCCTCAGTCTCACCT+TGG + tig0024026:78245-78264 None:intergenic 55.0%
CCAAGGTGAGACTGAGGATC+AGG - tig0024026:78242-78261 Msa1413430:CDS 55.0%
CCAAGAGCCTGATCAGGAAG+AGG + tig0024026:78345-78364 None:intergenic 55.0%
CCTCTTCCTGATCAGGCTCT+TGG - tig0024026:78342-78361 Msa1413430:CDS 55.0%
CCAAGACCAAGAGCCTGATC+AGG + tig0024026:78351-78370 None:intergenic 55.0%
CCTGATCAGGCTCTTGGTCT+TGG - tig0024026:78348-78367 Msa1413430:CDS 55.0%
ATGTGGCCATGTCCATGAGG+TGG + tig0024026:78462-78481 None:intergenic 55.0%
CGCAGTAGTGCCAGTACCAG+TGG - tig0024026:77939-77958 Msa1413430:CDS 60.0%
GGGAATGCCACAGATGGCTG+TGG - tig0024026:78129-78148 Msa1413430:CDS 60.0%
!! GGTGGCGCTACAGCTCTAGA+AGG + tig0024026:78444-78463 None:intergenic 60.0%
AGAGCTGTAGCGCCACCTCA+TGG - tig0024026:78447-78466 Msa1413430:CDS 60.0%
GTAGCGCCACCTCATGGACA+TGG - tig0024026:78453-78472 Msa1413430:CDS 60.0%
CGCAGTAGTGCCAGTACCAG+TGG - tig0024026:77939-77958 Msa1413430:CDS 60.0%
GGGAATGCCACAGATGGCTG+TGG - tig0024026:78129-78148 Msa1413430:CDS 60.0%
!! GGTGGCGCTACAGCTCTAGA+AGG + tig0024026:78444-78463 None:intergenic 60.0%
AGAGCTGTAGCGCCACCTCA+TGG - tig0024026:78447-78466 Msa1413430:CDS 60.0%
GTAGCGCCACCTCATGGACA+TGG - tig0024026:78453-78472 Msa1413430:CDS 60.0%
GGCAGCACCACCACAGCATG+AGG - tig0024026:78307-78326 Msa1413430:CDS 65.0%
GGCAGCACCACCACAGCATG+AGG - tig0024026:78307-78326 Msa1413430:CDS 65.0%
GCAGCAGCAGCAGCAGCAGC+AGG - tig0024026:78569-78588 Msa1413430:CDS 70.0%
GCAGCAGCAGCAGCAGCAGC+AGG - tig0024026:78569-78588 Msa1413430:CDS 70.0%
Chromosome Type Strat End Strand Name
tig0024026 gene 77739 78851 77739 ID=Msa1413430;Name=Msa1413430
tig0024026 mRNA 77739 78851 77739 ID=Msa1413430-mRNA-1;Parent=Msa1413430;Name=Msa1413430-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|370
tig0024026 exon 77739 78851 77739 ID=Msa1413430-mRNA-1:exon:21138;Parent=Msa1413430-mRNA-1
tig0024026 CDS 77739 78851 77739 ID=Msa1413430-mRNA-1:cds;Parent=Msa1413430-mRNA-1
Gene Sequence

>Msa1413430

ATGGAACTTTCCAACAGCCAAGATGGAGAAATCCCAATAAACCACAATAATAATAATAATAATAATAATAATAATAATAATTATGGTACTAGTAATGGACATGGTCATCACATGATTCACCATCATCATGTTCATGAACACCCTCATCCTCCTCCACTACACCACAATCACAGTAATATTATATCATCATCTTCTTCAGCCGCAGTAGTGCCAGTACCAGTGGCAGTAGTACCCCAAAACCCTTCAGTTAATGGCACAACCACTCCTATGCAAGCAGCAGCTGTTAGTACTGGTGTGGAATTAGATCACGATCAGTCCTCCTACAAGAAATCGTCATCAGTTGTGGTGAGGTACAGAGAATGTCTAAAGAATCATGCAGCTGCAATGGGAGGGAATGCCACAGATGGCTGTGGTGAGTTCATGCCCAGTGGTGAACAAGGAACCATTGAAGCACTCAACTGCTCTGCCTGCCATTGTCACAGAAACTTCCACAGAAAAGAAGTCCAAGGTGAGACTGAGGATCAGGAAAACTACCACCATCACTCAGCCTTCAACTTCAACTTCAACAGGCAGCACCACCACAGCATGAGGAAATTCATGTTACCTCTTCCTGATCAGGCTCTTGGTCTTGGCTACCAACACCACCACTCTACTCCTAATACAGCATCAGCAGCAACAGGTAACAACAACAACAACATTCTTCCTTCTAGAGCTGTAGCGCCACCTCATGGACATGGCCACATCATCATGCCATTCAACTACAACATACCTTCCGAATCTGATGAACAAGAAGAGGCAGCAGGTAGACTACATATTCATCATCATCATCAGCAGCAGCAGCAGCAGCAGCAGCAGGTAGTGATGAAGAAAAGGTTCAGGACAAAATTCACACAGGAACAGAAGGACAAAATGTTCAATTTTGCTGAGAAAGTTGGATGGAAATTTCAGAAGCAAGAGGAATCTGTGGTGCAACAGTTCTGCCAAGAGATTGGTGTGAAAAGAAGAGTGCTCAAGGTTTGGATGCATAACAATAAACACAATCTCGCCAAAAAACAACTTCAACATGAACATATTACTGCTAGTGCTAGTGCTAATCATCTCCCAACTCCATAA
Protein sequence

>Msa1413430

MELSNSQDGEIPINHNNNNNNNNNNNNYGTSNGHGHHMIHHHHVHEHPHPPPLHHNHSNIISSSSSAAVVPVPVAVVPQNPSVNGTTTPMQAAAVSTGVELDHDQSSYKKSSSVVVRYRECLKNHAAAMGGNATDGCGEFMPSGEQGTIEALNCSACHCHRNFHRKEVQGETEDQENYHHHSAFNFNFNRQHHHSMRKFMLPLPDQALGLGYQHHHSTPNTASAATGNNNNNILPSRAVAPPHGHGHIIMPFNYNIPSESDEQEEAAGRLHIHHHHQQQQQQQQQVVMKKRFRTKFTQEQKDKMFNFAEKVGWKFQKQEESVVQQFCQEIGVKRRVLKVWMHNNKHNLAKKQLQHEHITASASANHLPTP*