AlfalfaGEDB | Msa0951460

M. sativa cultivar zhongmu-4 / Msa0951460

KEGG: K19747
GO: GO:0000902 GO:0000904 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009888 GO:0009987 GO:0010026 GO:0010090 GO:0010143 GO:0016043 GO:0030154 GO:0032502 GO:0032989 GO:0043170 GO:0044424 GO:0044464 GO:0048468 GO:0048856 GO:0048869 GO:0051179 GO:0071704 GO:0071840 GO:0090558 GO:0090626 GO:1901576
PFAM: PF02458.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0951460	sp\|Q9FF86\|DCR_ARATH	62.658	474	139	6	45	505	1	449	0.0	599

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0951460	A0A396HFL3	97.633	507	12	0	1	507	1	507	0.0	1015

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003470	Msa0951460	0.820203	7.764167e-53	-8.615850e-47
Msa0004350	Msa0951460	0.814949	1.187230e-51	-8.615850e-47
Msa0005050	Msa0951460	0.801517	8.676288e-49	-8.615850e-47
Msa0012460	Msa0951460	0.804065	2.582612e-49	-8.615850e-47
Msa0017120	Msa0951460	0.843956	1.018697e-58	-8.615850e-47
Msa0018220	Msa0951460	0.841623	4.245982e-58	-8.615850e-47
Msa0024680	Msa0951460	0.813221	2.856811e-51	-8.615850e-47
Msa0030730	Msa0951460	0.820515	6.586403e-53	-8.615850e-47
Msa0032120	Msa0951460	0.805972	1.031338e-49	-8.615850e-47
Msa0032400	Msa0951460	0.807096	5.972453e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0951460	MtrunA17_Chr6g0467861	97.633	507	12	0	1	507	1	507	0.0	1015
Msa0951460	MtrunA17_Chr5g0436621	31.384	513	257	11	49	504	8	482	5.31e-76	247
Msa0951460	MtrunA17_Chr4g0005941	33.063	493	292	10	44	507	3	486	3.11e-74	243
Msa0951460	MtrunA17_Chr3g0113181	30.522	498	275	8	41	504	6	466	1.85e-70	232
Msa0951460	MtrunA17_Chr3g0134041	30.672	476	280	11	47	507	7	447	3.82e-64	215
Msa0951460	MtrunA17_Chr3g0134121	33.195	482	268	13	41	504	2	447	7.68e-64	215
Msa0951460	MtrunA17_Chr3g0134111	31.435	474	282	11	47	507	7	450	1.04e-60	206
Msa0951460	MtrunA17_Chr4g0053111	31.381	478	273	12	46	504	3	444	7.30e-60	204
Msa0951460	MtrunA17_Chr3g0134061	29.412	493	284	10	44	507	2	459	1.64e-59	203
Msa0951460	MtrunA17_Chr3g0134091	30.947	475	277	11	46	503	5	445	4.70e-59	202

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0951460	AT5G23940.1	62.658	474	139	6	45	505	1	449	0.0	599
Msa0951460	AT2G39980.1	31.373	510	265	9	46	507	7	479	3.17e-75	245
Msa0951460	AT5G01210.1	32.406	503	264	7	44	507	3	468	9.79e-74	241
Msa0951460	AT5G42830.1	33.843	458	260	13	57	504	21	445	3.66e-65	218
Msa0951460	AT5G07870.1	30.705	482	290	9	41	504	2	457	2.32e-59	203
Msa0951460	AT5G07860.1	30.561	481	268	12	47	504	8	445	2.44e-59	202
Msa0951460	AT5G67150.1	31.078	473	282	11	47	507	5	445	6.63e-59	201
Msa0951460	AT3G50270.1	29.140	477	287	11	47	507	6	447	4.23e-57	197
Msa0951460	AT3G50280.1	30.290	482	268	16	46	504	3	439	7.31e-57	196
Msa0951460	AT5G07850.1	30.928	485	283	17	41	507	2	452	2.85e-55	192

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Find 147 sgRNAs with CRISPR-Local

Find 540 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCATTCTCAACATTTC+AGG	0.271160	6_3:-42407290	Msa0951460:CDS
AAAGAAGCACCGAATTGTGG+CGG	0.621574	6_3:+42407082	None:intergenic
AAATTAGGAAAAGATGAAGA+GGG	0.635748	6_3:-42410189	Msa0951460:CDS
AAATTCAAGATTCCACTATA+AGG	0.356072	6_3:+42410042	None:intergenic
AACATTGGAGCCTGAGGCTA+TGG	0.403215	6_3:-42406795	Msa0951460:CDS
AACCAAACCCTTCCATCAGA+CGG	0.617474	6_3:-42407316	Msa0951460:CDS
AACCGTCTGATGGAAGGGTT+TGG	0.457328	6_3:+42407314	None:intergenic
AAGAAGCACCGAATTGTGGC+GGG	0.555266	6_3:+42407083	None:intergenic
AAGCACCGAATTGTGGCGGG+TGG	0.568822	6_3:+42407086	None:intergenic
AAGCCAGAGATTGTGAGAAG+TGG	0.561140	6_3:-42406893	Msa0951460:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGAAACCTGAGGAAAAAATT+GGG	-	chr6_3:42406671-42406690	Msa0951460:three_prime_UTR	30.0%
TGAGGAAAAAATTGGGAAAA+AGG	-	chr6_3:42406678-42406697	Msa0951460:three_prime_UTR	30.0%
AGTAAAAAGCTAAGTAAGGT+AGG	+	chr6_3:42406726-42406745	None:intergenic	30.0%
AATGTTGAGAAACTCAAGAA+TGG	-	chr6_3:42406790-42406809	Msa0951460:CDS	30.0%
ATGTTGAGAAACTCAAGAAT+GGG	-	chr6_3:42406791-42406810	Msa0951460:CDS	30.0%
AGGAATCAATTCCTTCAAAA+TGG	+	chr6_3:42406983-42407002	None:intergenic	30.0%
TGCCAAATACTATCTAAATG+TGG	-	chr6_3:42407507-42407526	Msa0951460:CDS	30.0%
AGATTGAAAACCACAAAACA+AGG	-	chr6_3:42407551-42407570	Msa0951460:CDS	30.0%
GATTGAAAACCACAAAACAA+GGG	-	chr6_3:42407552-42407571	Msa0951460:CDS	30.0%
CTTCTACTCTAATTGTTACT+TGG	+	chr6_3:42407792-42407811	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_3	gene	42406484	42410580	42406484	ID=Msa0951460;Name=Msa0951460
chr6_3	mRNA	42406484	42410580	42406484	ID=Msa0951460-mRNA-1;Parent=Msa0951460;Name=Msa0951460-mRNA-1;_AED=0.00;_eAED=0.00;_QI=24\|1\|1\|1\|1\|1\|2\|270\|507
chr6_3	exon	42406484	42407728	42406484	ID=Msa0951460-mRNA-1:exon:9921;Parent=Msa0951460-mRNA-1
chr6_3	exon	42410008	42410580	42410008	ID=Msa0951460-mRNA-1:exon:9920;Parent=Msa0951460-mRNA-1
chr6_3	five_prime_UTR	42410557	42410580	42410557	ID=Msa0951460-mRNA-1:five_prime_utr;Parent=Msa0951460-mRNA-1
chr6_3	CDS	42410008	42410556	42410008	ID=Msa0951460-mRNA-1:cds;Parent=Msa0951460-mRNA-1
chr6_3	CDS	42406754	42407728	42406754	ID=Msa0951460-mRNA-1:cds;Parent=Msa0951460-mRNA-1
chr6_3	three_prime_UTR	42406484	42406753	42406484	ID=Msa0951460-mRNA-1:three_prime_utr;Parent=Msa0951460-mRNA-1

Gene Sequence

>Msa0951460

ATGCCAACAACACTTTTCTATAAAAGTCCTCAAAAATCACGTCTCACACACAAATCAAACAAATTCCAAGAAAAACTTCTAAAAATTTCATTCAACAAAATGTCAAGTGAAGTAGTCAAGAATGAAGAATCTCTAAATGTGAAAATCACTAGCAAAAGCTATGTGAAACCTGAGGAAAAAATTGGGAAAAAGGAGTATCAATTGGTCACATTTGACCTACCTTACTTAGCTTTTTACTACAACCAAAAATTAATTTTTTACAAAGGAGGTAACTTTGAAGAAAATGTTGAGAAACTCAAGAATGGGCTTAGTGTAGTTTTGAAGGAGTTTCATCAACTAGCTGGTAAATTAGGAAAAGATGAAGAGGGTGTGTTTTTTGTTGAATATGATGATGACTTGGTTGGTGTTGAAGTTGTTGAAGCTGTTGCTGATGAGATTGGTGTTGAAGATCTAACTGTTGCTGAAAGTAGTGCCATTTTGAAGGAATTGATTCCTTATAGTGGAATCTTGAATTTGGAAGGCATGCATAGACCCTTGTTGGCTGTTCAGCTAACAAAGCTCAAAGATGGGCTTGCAATGGGCTTGGCTTTTAACCATGCTGTCCTTGATGGAACATCCACGTGGCACTTTATGACTTCATGGGCCGAGATATGCAGTGGTGGACCCATCACAACAGCCCAACCATTCTTGGAACGAACCAAGGCTCGAAACACACGTGTGAAGCTTGACCTCTCCCTACCCGAGCCCAAAGGCCCGCCACCATCAGCCAACGGTGAGGTAAAGGATGAACAAAATGGCCCAGCACCATCAGCCAATGGTGAGGCAAAGGTTGAACCCGAAGGCCCGCCACCATCAGTCAAAGGTGAGGCGAGGCCCATACTTAGGGAAAAGATCTTCAAATTTTCCGAGTCAGCCATCGACAAAATCAAGTCAACAGTCAACCAAACCCTTCCATCAGACGGTTCAAAACCATTCTCAACATTTCAGGCTCTCTCCACTCATATTTGGCGCCACGTGAGCCTTGCACGTGACCTAAAACCCGAAGACTACACAGTGTTCACCGTCTTCGCAGACTGCAGAAAACGAGTGAACCCACCAATGCCGGAGGCATACTTTGGAAACCTAATTCAAGCTATATTCACCGTCACGGCTGCTGGGCTTTTGGCGGCCCACCCGCCACAATTCGGTGCTTCTTTGATCCAAAAGGTTATTGAAGGCCATGATGCTAAAGCTATTGATGAACGTAACAAAGAGTGGGAGAGTGCACCCAAGATTTTTCAATTCAAAGATGCTGGTGTGAATTGTGTTGCAGTTGGAAGTTCACCTAGGTTTAAGGTTTATGACATTGATTTTGGATTTGGGAAGCCAGAGATTGTGAGAAGTGGGACCAATAACAAATTTGATGGAATGATTTATTTGTATCCTGGCAAGAGTGGAGGAAGGAGCATTGATGTTGAATTAACATTGGAGCCTGAGGCTATGGGAAGGTTGGAGCAAGATAAGGAATTTCTTATGGAAGTATAA

Protein sequence

>Msa0951460

MPTTLFYKSPQKSRLTHKSNKFQEKLLKISFNKMSSEVVKNEESLNVKITSKSYVKPEEKIGKKEYQLVTFDLPYLAFYYNQKLIFYKGGNFEENVEKLKNGLSVVLKEFHQLAGKLGKDEEGVFFVEYDDDLVGVEVVEAVADEIGVEDLTVAESSAILKELIPYSGILNLEGMHRPLLAVQLTKLKDGLAMGLAFNHAVLDGTSTWHFMTSWAEICSGGPITTAQPFLERTKARNTRVKLDLSLPEPKGPPPSANGEVKDEQNGPAPSANGEAKVEPEGPPPSVKGEARPILREKIFKFSESAIDKIKSTVNQTLPSDGSKPFSTFQALSTHIWRHVSLARDLKPEDYTVFTVFADCRKRVNPPMPEAYFGNLIQAIFTVTAAGLLAAHPPQFGASLIQKVIEGHDAKAIDERNKEWESAPKIFQFKDAGVNCVAVGSSPRFKVYDIDFGFGKPEIVRSGTNNKFDGMIYLYPGKSGGRSIDVELTLEPEAMGRLEQDKEFLMEV*