AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1426080

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1426080 MtrunA17_Chr7g0263731 97.650 468 11 0 1 468 1 468 0.0 929
Msa1426080 MtrunA17_Chr1g0181581 69.665 478 121 11 1 467 1 465 0.0 597
Msa1426080 MtrunA17_Chr6g0462191 50.655 458 189 9 1 453 1 426 2.27e-120 358
Msa1426080 MtrunA17_Chr1g0200161 39.096 509 239 11 1 465 1 482 2.81e-97 301
Msa1426080 MtrunA17_Chr4g0045641 35.294 357 178 15 108 439 146 474 5.68e-43 159
Msa1426080 MtrunA17_Chr8g0346021 31.888 392 202 13 94 438 113 486 5.27e-42 157
Msa1426080 MtrunA17_Chr4g0025441 47.917 192 96 2 107 298 95 282 9.36e-42 154
Msa1426080 MtrunA17_Chr4g0020671 31.808 459 209 23 3 439 4 380 4.72e-39 146
Msa1426080 MtrunA17_Chr2g0283321 41.420 169 96 2 107 275 62 227 9.29e-37 137
Msa1426080 MtrunA17_Chr7g0273861 28.539 438 219 18 1 411 1 371 7.98e-32 126
Msa1426080 MtrunA17_Chr3g0135851 44.385 187 89 5 103 275 80 265 1.42e-31 125
Msa1426080 MtrunA17_Chr2g0316401 35.928 167 106 1 109 275 75 240 6.23e-31 121
Msa1426080 MtrunA17_Chr1g0146931 39.109 202 97 4 106 281 120 321 1.49e-28 117
Msa1426080 MtrunA17_Chr3g0126901 27.352 457 219 15 1 429 1 372 5.23e-26 110
Msa1426080 MtrunA17_Chr1g0204901 31.950 241 130 8 1 211 1 237 1.01e-19 91.7
Msa1426080 MtrunA17_Chr8g0340761 31.496 254 125 12 1 216 1 243 1.17e-18 87.8
Msa1426080 MtrunA17_Chr8g0342551 33.681 288 166 8 1 283 1 268 1.76e-18 87.4
Msa1426080 MtrunA17_Chr1g0203711 27.937 315 174 10 107 411 114 385 2.47e-18 87.0
Msa1426080 MtrunA17_Chr6g0472551 53.165 79 36 1 107 184 115 193 3.61e-18 86.7
Msa1426080 MtrunA17_Chr2g0330051 32.000 325 150 10 105 409 69 342 4.09e-18 86.3
Msa1426080 MtrunA17_Chr8g0389781 55.556 72 32 0 103 174 121 192 6.50e-18 85.9
Msa1426080 MtrunA17_Chr2g0330921 48.780 82 36 1 97 172 80 161 1.14e-16 81.3
Msa1426080 MtrunA17_Chr1g0172611 27.143 280 180 10 1 265 1 271 1.37e-15 78.2
Msa1426080 MtrunA17_Chr7g0273951 50.000 74 37 0 110 183 108 181 1.68e-15 78.2
Msa1426080 MtrunA17_Chr4g0060381 28.507 221 118 3 54 272 61 243 3.21e-15 78.2
Msa1426080 MtrunA17_Chr1g0166431 43.000 100 56 1 108 206 117 216 2.60e-14 74.7
Msa1426080 MtrunA17_Chr8g0371131 48.485 66 34 0 106 171 69 134 8.56e-14 68.6
Msa1426080 MtrunA17_Chr3g0130441 36.842 114 65 3 251 362 1 109 2.08e-12 64.3
Msa1426080 MtrunA17_Chr1g0156121 31.481 162 84 5 108 267 124 260 2.85e-12 68.9
Msa1426080 MtrunA17_Chr4g0057321 46.970 66 35 0 106 171 86 151 1.33e-11 66.2
Msa1426080 MtrunA17_Chr3g0130451 52.459 61 26 1 169 226 47 107 2.48e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1426080 AT5G03040.1 52.245 490 183 15 1 468 1 461 1.12e-146 427
Msa1426080 AT5G03040.3 52.245 490 183 15 1 468 1 461 1.12e-146 427
Msa1426080 AT5G03040.2 52.245 490 183 15 1 468 1 461 1.12e-146 427
Msa1426080 AT3G52290.1 46.383 470 190 14 1 456 1 422 2.33e-111 336
Msa1426080 AT3G09710.1 48.000 475 200 15 1 456 1 447 5.19e-111 335
Msa1426080 AT3G09710.2 46.626 489 200 16 1 456 1 461 1.02e-107 328
Msa1426080 AT2G26410.2 37.126 334 161 8 106 434 146 435 8.75e-56 194
Msa1426080 AT2G26410.1 37.126 334 161 8 106 434 146 435 8.75e-56 194
Msa1426080 AT3G22190.3 44.177 249 123 8 86 323 63 306 2.86e-40 150
Msa1426080 AT3G22190.2 44.177 249 123 8 86 323 63 306 2.86e-40 150
Msa1426080 AT3G22190.4 44.177 249 123 8 86 323 63 306 2.86e-40 150
Msa1426080 AT3G22190.1 44.177 249 123 8 86 323 63 306 2.86e-40 150
Msa1426080 AT3G59690.2 33.770 305 152 8 111 411 172 430 1.59e-37 144
Msa1426080 AT3G59690.1 33.770 305 152 8 111 411 172 430 1.59e-37 144
Msa1426080 AT2G33990.1 40.698 172 100 1 107 278 46 215 1.82e-37 138
Msa1426080 AT1G01110.2 29.459 499 263 22 1 440 1 469 1.77e-36 141
Msa1426080 AT2G26180.1 46.667 195 89 4 95 275 65 258 1.88e-36 139
Msa1426080 AT2G43680.1 31.085 341 175 11 105 439 320 606 3.11e-35 139
Msa1426080 AT2G43680.4 31.085 341 175 11 105 439 319 605 3.37e-35 139
Msa1426080 AT2G43680.2 31.085 341 175 11 105 439 319 605 3.37e-35 139
Msa1426080 AT2G43680.3 31.085 341 175 11 105 439 319 605 3.37e-35 139
Msa1426080 AT2G43680.5 31.085 341 175 11 105 439 319 605 3.37e-35 139
Msa1426080 AT4G00820.1 30.833 360 210 13 106 440 130 475 2.42e-34 135
Msa1426080 AT3G15050.1 37.870 169 104 1 108 276 50 217 2.89e-33 127
Msa1426080 AT3G49260.1 31.046 306 174 10 1 275 1 300 8.35e-31 124
Msa1426080 AT3G49260.4 31.046 306 174 10 1 275 1 300 8.35e-31 124
Msa1426080 AT3G49260.2 31.046 306 174 10 1 275 1 300 8.35e-31 124
Msa1426080 AT5G13460.1 29.508 305 158 10 1 279 1 274 6.73e-30 121
Msa1426080 AT3G49260.3 30.293 307 176 10 1 275 1 301 2.24e-29 120
Msa1426080 AT1G72670.1 34.906 212 124 5 95 295 74 282 2.16e-28 117
Msa1426080 AT4G10640.1 28.189 486 189 20 1 436 1 376 2.77e-22 99.4
Msa1426080 AT1G17480.1 36.306 157 87 3 106 260 92 237 9.21e-21 94.0
Msa1426080 AT4G14750.1 34.884 129 77 1 90 218 87 208 3.68e-18 86.7
Msa1426080 AT4G14750.2 34.884 129 77 1 90 218 146 267 5.33e-18 86.7
Msa1426080 AT4G14750.3 34.884 129 77 1 90 218 146 267 6.91e-18 86.3
Msa1426080 AT4G29150.1 43.434 99 56 0 102 200 125 223 2.72e-17 84.0
Msa1426080 AT3G16490.1 43.000 100 52 2 101 197 100 197 2.88e-17 84.0
Msa1426080 AT1G18840.1 35.659 129 79 2 61 188 64 189 3.88e-16 81.3
Msa1426080 AT1G18840.3 35.659 129 79 2 61 188 64 189 3.88e-16 81.3
Msa1426080 AT1G18840.2 35.659 129 79 2 61 188 64 189 3.88e-16 81.3
Msa1426080 AT5G35670.1 35.542 166 94 5 107 270 159 313 4.07e-16 80.9
Msa1426080 AT1G74690.1 31.953 169 85 3 108 272 113 255 5.53e-16 80.9
Msa1426080 AT5G07240.1 61.017 59 23 0 110 168 108 166 1.79e-15 78.6
Msa1426080 AT4G23060.1 42.157 102 57 1 82 181 139 240 2.21e-15 78.6
Msa1426080 AT5G07240.2 61.017 59 23 0 110 168 154 212 2.22e-15 78.6
Msa1426080 AT1G51960.1 54.839 62 28 0 110 171 102 163 6.11e-15 76.6
Msa1426080 AT5G62070.1 59.649 57 23 0 106 162 115 171 9.39e-15 76.3
Msa1426080 AT3G51380.1 50.000 64 32 0 106 169 35 98 1.68e-14 69.7
Msa1426080 AT5G03960.1 36.000 150 65 3 100 232 99 234 7.40e-14 73.6
Msa1426080 AT5G03960.3 36.000 150 65 3 100 232 100 235 7.92e-14 73.6
Msa1426080 AT5G03960.2 36.000 150 65 3 100 232 100 235 7.92e-14 73.6
Msa1426080 AT1G14380.7 27.928 222 116 7 70 274 42 236 1.81e-11 66.6
Msa1426080 AT1G14380.6 27.928 222 116 7 70 274 42 236 2.22e-11 66.6
Msa1426080 AT1G14380.5 27.928 222 116 7 70 274 42 236 2.22e-11 66.6
Msa1426080 AT1G14380.2 27.928 222 116 7 70 274 42 236 2.22e-11 66.6
Msa1426080 AT1G14380.1 27.928 222 116 7 70 274 42 236 3.26e-11 66.2
Msa1426080 AT1G14380.3 27.928 222 116 7 70 274 42 236 3.26e-11 66.2
Msa1426080 AT1G14380.4 27.928 222 116 7 70 274 42 236 3.26e-11 66.2
Msa1426080 AT3G49380.1 54.237 59 27 0 106 164 108 166 4.33e-11 64.7

Find 145 sgRNAs with CRISPR-Local

Find 255 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTCCCCATTATCTTCT+TGG 0.102885 tig0025942:-25801 None:intergenic
CTCATACTTGCTCAGTAATT+TGG 0.160600 tig0025942:-26882 None:intergenic
CACAGGTAACTCTGCATTTA+TGG 0.176562 tig0025942:-28544 None:intergenic
GACCTTGAGGGGAAGAAATT+TGG 0.205051 tig0025942:+28816 Msa1426080:three_prime_UTR
TTTACAGGAACATGTATTAA+AGG 0.248528 tig0025942:+25291 Msa1426080:five_prime_UTR
CAATTTGTTCTTTAGATTGT+AGG 0.261072 tig0025942:-26856 None:intergenic
AGCTGGGTGAGCCAGGATTT+TGG 0.263792 tig0025942:-28137 None:intergenic
TGATATAAAGGATCAAATTA+AGG 0.280243 tig0025942:+25774 Msa1426080:intron
CTGTACACTTGCCATGCTTT+TGG 0.281192 tig0025942:-28259 None:intergenic
AAAATACAAATTGATTAATA+TGG 0.294508 tig0025942:-28773 None:intergenic
ATTGCTGCCACTTCATCCTT+TGG 0.307055 tig0025942:-26211 None:intergenic
TCAGATCTTTCACTTCATTC+AGG 0.319208 tig0025942:+25386 Msa1426080:five_prime_UTR
TAATGGGGAAGAAGGGAAAT+TGG 0.319680 tig0025942:+25810 Msa1426080:exon
AGTTCGAGCCGCAGCATTAT+AGG 0.323895 tig0025942:+28048 Msa1426080:CDS
TTCCGCAGGCACTCTATTGC+CGG 0.327399 tig0025942:+28291 Msa1426080:CDS
AAAAGTATTAATGAAATGTT+TGG 0.336829 tig0025942:-28723 None:intergenic
AGTCCATTAGCAAAAGAAAA+TGG 0.343691 tig0025942:+28417 Msa1426080:CDS
TCAGTTAATTTAATATCTTC+TGG 0.345648 tig0025942:-26055 None:intergenic
AAGGAATGCCAAGAAGATAA+TGG 0.348066 tig0025942:+25793 Msa1426080:exon
AGGAATGCCAAGAAGATAAT+GGG 0.352188 tig0025942:+25794 Msa1426080:exon
TCCATCAGTTCCAAGTTATA+TGG 0.368788 tig0025942:+28350 Msa1426080:CDS
CGGCTTAGTAGGAGTTGAAT+TGG 0.370892 tig0025942:-28166 None:intergenic
CTTGGCCTCGCAGGCGAAGC+CGG 0.371444 tig0025942:-28489 None:intergenic
TTAGGCAAGAAGAGCATTGC+GGG 0.387422 tig0025942:+26492 Msa1426080:intron
AGAAAATGGCAAACAAGAAA+AGG 0.397205 tig0025942:+28431 Msa1426080:CDS
GGCTTATGCATTCACTCATC+AGG 0.397924 tig0025942:+26931 Msa1426080:CDS
TGGATCCCACCAATCCTACT+TGG 0.400459 tig0025942:+27932 Msa1426080:CDS
GACTGAAAGCTGGGTGAGCC+AGG 0.404427 tig0025942:-28144 None:intergenic
TTTATGGCGGTTTCTACCTT+CGG 0.410128 tig0025942:-28528 None:intergenic
ATCTATGAATCCAATGTTTG+TGG 0.410976 tig0025942:+27912 Msa1426080:CDS
TCCATAACCCAACTTCCCTT+TGG 0.416087 tig0025942:-28228 None:intergenic
ATGGATGGCTGCCCGACCTT+GGG 0.416101 tig0025942:+27975 Msa1426080:CDS
TTCAGAGTTGAGCTGGAAAC+GGG 0.418428 tig0025942:-28094 None:intergenic
AAACAAGAAAAGGGGTCCTT+TGG 0.420323 tig0025942:+28441 Msa1426080:CDS
AGAGCATTGCGGGCTTTAAG+AGG 0.426239 tig0025942:+26502 Msa1426080:CDS
CTGATTATTATATAATCAAC+TGG 0.427029 tig0025942:-28580 None:intergenic
TGCAATCTCAGATTCGTTCT+AGG 0.427507 tig0025942:+26614 Msa1426080:CDS
CATTATAGATTGGGGAGGAA+TGG 0.428913 tig0025942:+26825 Msa1426080:intron
TCCAAAGGGAAGTTGGGTTA+TGG 0.429384 tig0025942:+28227 Msa1426080:CDS
GAAAATGGCAAACAAGAAAA+GGG 0.429410 tig0025942:+28432 Msa1426080:CDS
TTGGCCTCGCAGGCGAAGCC+GGG 0.432715 tig0025942:-28488 None:intergenic
TTCTTTACCATTGACCTTGA+GGG 0.433200 tig0025942:+28804 Msa1426080:three_prime_UTR
GAAGCAGAACCCGGCTTAGT+AGG 0.447007 tig0025942:-28177 None:intergenic
ATTATAGATTGGGGAGGAAT+GGG 0.448809 tig0025942:+26826 Msa1426080:intron
TAGGAGGGTCAGAATGTTAG+AGG 0.452802 tig0025942:+26633 Msa1426080:CDS
TTTCTTCCCCTCAAGGTCAA+TGG 0.453129 tig0025942:-28811 None:intergenic
TTTCAGAGTTGAGCTGGAAA+CGG 0.462924 tig0025942:-28095 None:intergenic
ATATCTTCTGGCGGAGGAAG+AGG 0.467550 tig0025942:-26043 None:intergenic
CAGAATGTTAGAGGAGGATC+AGG 0.467779 tig0025942:+26642 Msa1426080:CDS
GATGGATGGCTGCCCGACCT+TGG 0.469154 tig0025942:+27974 Msa1426080:CDS
CATGTATTAAAGGTGTGGTT+TGG 0.469410 tig0025942:+25301 Msa1426080:five_prime_UTR
CTAGCAACTGAAGCAGAACC+CGG 0.473296 tig0025942:-28186 None:intergenic
GGCGAGTCCAAAGGGAAGTT+GGG 0.474248 tig0025942:+28221 Msa1426080:CDS
CAAATTACTGAGCAAGTATG+AGG 0.474416 tig0025942:+26883 Msa1426080:CDS
AATGTATTTCAGAGTTGAGC+TGG 0.481876 tig0025942:-28101 None:intergenic
GGATCCCACCAATCCTACTT+GGG 0.483131 tig0025942:+27933 Msa1426080:CDS
CAAGAAGATAATGGGGAAGA+AGG 0.486516 tig0025942:+25802 Msa1426080:exon
TAATACATGTTCCTGTAAAG+AGG 0.486866 tig0025942:-25287 None:intergenic
GAAACTCTTGATTCTAAATG+TGG 0.487636 tig0025942:+28963 Msa1426080:three_prime_UTR
CGGGTTCTGCTTCAGTTGCT+AGG 0.487745 tig0025942:+28187 Msa1426080:CDS
CAATTCAACTCCTACTAAGC+CGG 0.488786 tig0025942:+28167 Msa1426080:CDS
AGGCGAGTCCAAAGGGAAGT+TGG 0.492154 tig0025942:+28220 Msa1426080:CDS
CTTGTTGATGAAACACAGAA+TGG 0.495295 tig0025942:-28883 None:intergenic
CTGAAGAAGGCGAGTCCAAA+GGG 0.500013 tig0025942:+28213 Msa1426080:CDS
AGTTGAATTGGACTGAAAGC+TGG 0.502940 tig0025942:-28154 None:intergenic
GCTGTCTGAACAGAACGAAC+AGG 0.506676 tig0025942:-26142 None:intergenic
AGCGGAGGGTACTCATGGCT+TGG 0.509205 tig0025942:-26570 None:intergenic
AGGACAGAAATCATTAAAGT+AGG 0.510647 tig0025942:+28609 Msa1426080:three_prime_UTR
ATTGCGGGCTTTAAGAGGGC+TGG 0.511040 tig0025942:+26507 Msa1426080:CDS
CGACTCAATCTGCGAAAGCT+AGG 0.512605 tig0025942:+28376 Msa1426080:CDS
GGTGGACCTGAATGACGCCT+TGG 0.514462 tig0025942:-28507 None:intergenic
TATTAAAGGTGTGGTTTGGT+TGG 0.514474 tig0025942:+25305 Msa1426080:five_prime_UTR
AGCTCTTACAGAAGCACGCA+AGG 0.515912 tig0025942:+26677 Msa1426080:CDS
TCCGCAGGCACTCTATTGCC+GGG 0.521984 tig0025942:+28292 Msa1426080:CDS
CCAGTAGCGAGCCAAAATCC+TGG 0.525286 tig0025942:+28126 Msa1426080:CDS
AGCTCTCAGCCCTGACTCAA+AGG 0.528686 tig0025942:+25850 Msa1426080:CDS
GGTGAGATTGAAAACATTGA+TGG 0.529438 tig0025942:+26528 Msa1426080:CDS
TACAGTCAGATCGGTTCCGC+AGG 0.535268 tig0025942:+28277 Msa1426080:CDS
AATACATGTTCCTGTAAAGA+GGG 0.535430 tig0025942:-25286 None:intergenic
GTTCATGTTGATCTTGTGAC+GGG 0.537235 tig0025942:+28838 Msa1426080:three_prime_UTR
AACCAAATTTCTTCCCCTCA+AGG 0.538043 tig0025942:-28818 None:intergenic
CATTGAGCGGAGGGTACTCA+TGG 0.539242 tig0025942:-26575 None:intergenic
AATTCAACTCCTACTAAGCC+GGG 0.541175 tig0025942:+28168 Msa1426080:CDS
ACCATATAACTTGGAACTGA+TGG 0.542002 tig0025942:-28351 None:intergenic
TTGGTGGGATCCACAAACAT+TGG 0.547737 tig0025942:-27922 None:intergenic
TTGTAATTTCAGCAACATGA+TGG 0.550799 tig0025942:-26099 None:intergenic
TATATAATAATCAGAGTGAA+AGG 0.551583 tig0025942:+28589 Msa1426080:three_prime_UTR
ATGGCGGTTTCTACCTTCGG+TGG 0.551866 tig0025942:-28525 None:intergenic
TGAGTCGGCACCATATAACT+TGG 0.553119 tig0025942:-28360 None:intergenic
GATCCCACCAATCCTACTTG+GGG 0.554025 tig0025942:+27934 Msa1426080:CDS
CATTCTGTGTTTCATCAACA+AGG 0.560883 tig0025942:+28884 Msa1426080:three_prime_UTR
ATCATCTCTAACTGATGACC+CGG 0.566819 tig0025942:-28310 None:intergenic
ATTCTTTACCATTGACCTTG+AGG 0.568860 tig0025942:+28803 Msa1426080:three_prime_UTR
TACAACTGTGGTGGATGTCG+AGG 0.573197 tig0025942:+26117 Msa1426080:CDS
GAGCATTGCGGGCTTTAAGA+GGG 0.574907 tig0025942:+26503 Msa1426080:CDS
GGTTCATGTTGATCTTGTGA+CGG 0.575573 tig0025942:+28837 Msa1426080:three_prime_UTR
GCTGTATCGATTTCTACAGA+GGG 0.577428 tig0025942:-26016 None:intergenic
AGGAACATGTATTAAAGGTG+TGG 0.580118 tig0025942:+25296 Msa1426080:five_prime_UTR
TAACGTCTGCATTGAGCGGA+GGG 0.581741 tig0025942:-26584 None:intergenic
AATTTAATATCTTCTGGCGG+AGG 0.588204 tig0025942:-26049 None:intergenic
AAGAAGATAATGGGGAAGAA+GGG 0.590179 tig0025942:+25803 Msa1426080:exon
AGATTGAAAACATTGATGGA+AGG 0.590382 tig0025942:+26532 Msa1426080:CDS
ACTGAAGAAGGCGAGTCCAA+AGG 0.594202 tig0025942:+28212 Msa1426080:CDS
ATCCCACCAATCCTACTTGG+GGG 0.597567 tig0025942:+27935 Msa1426080:CDS
AGTTGCTAGGAAACTGAAGA+AGG 0.598507 tig0025942:+28200 Msa1426080:CDS
CTGGCCACACCATTGCTCAC+AGG 0.598982 tig0025942:-28561 None:intergenic
TGCTGTATCGATTTCTACAG+AGG 0.603089 tig0025942:-26017 None:intergenic
TGGTAAACCAAAGGATGAAG+TGG 0.603448 tig0025942:+26204 Msa1426080:CDS
TCTTTACCATTGACCTTGAG+GGG 0.607112 tig0025942:+28805 Msa1426080:three_prime_UTR
ATCTCAGATTCGTTCTAGGA+GGG 0.616350 tig0025942:+26618 Msa1426080:CDS
AATCTCAGATTCGTTCTAGG+AGG 0.623684 tig0025942:+26617 Msa1426080:CDS
GATTGAAAACATTGATGGAA+GGG 0.623713 tig0025942:+26533 Msa1426080:CDS
CTAACGTCTGCATTGAGCGG+AGG 0.624271 tig0025942:-26585 None:intergenic
TGGCAAGTGTACAGTCAGAT+CGG 0.624328 tig0025942:+28268 Msa1426080:CDS
CTAGCTTTCGCAGATTGAGT+CGG 0.624424 tig0025942:-28375 None:intergenic
GGATGATGACTCCAAAAGCA+TGG 0.624895 tig0025942:+28248 Msa1426080:CDS
CATGTTGATCTTGTGACGGG+AGG 0.627453 tig0025942:+28841 Msa1426080:three_prime_UTR
GTTGAATTGGACTGAAAGCT+GGG 0.631003 tig0025942:-28153 None:intergenic
AAGGGAGCTTGAAACCATGC+GGG 0.632453 tig0025942:+26696 Msa1426080:CDS
GCCCGACCTTGGGAGAGCCG+TGG 0.632629 tig0025942:+27985 Msa1426080:CDS
ACCCGGCAATAGAGTGCCTG+CGG 0.633713 tig0025942:-28293 None:intergenic
GAAAGAAACCCATACCCGCA+TGG 0.636453 tig0025942:-26710 None:intergenic
TCTTCTGGCGGAGGAAGAGG+CGG 0.642359 tig0025942:-26040 None:intergenic
AGGTAACTCTGCATTTATGG+CGG 0.642380 tig0025942:-28541 None:intergenic
GCGTCATTCAGGTCCACCGA+AGG 0.644936 tig0025942:+28512 Msa1426080:CDS
TATGCGAAGAGAAAGAGCAC+TGG 0.647271 tig0025942:+26910 Msa1426080:CDS
TGCTGAAATTACAACTGTGG+TGG 0.649384 tig0025942:+26108 Msa1426080:CDS
GGAATGCCAAGAAGATAATG+GGG 0.650329 tig0025942:+25795 Msa1426080:exon
TCGAGCCGCAGCATTATAGG+TGG 0.650702 tig0025942:+28051 Msa1426080:CDS
AAAATGGCAAACAAGAAAAG+GGG 0.651135 tig0025942:+28433 Msa1426080:CDS
TTTAGGCAAGAAGAGCATTG+CGG 0.651340 tig0025942:+26491 Msa1426080:intron
TTTCTCCACCTATAATGCTG+CGG 0.652765 tig0025942:-28056 None:intergenic
GTTAATTTAATATCTTCTGG+CGG 0.660808 tig0025942:-26052 None:intergenic
GAGGGTCAGAATGTTAGAGG+AGG 0.664630 tig0025942:+26636 Msa1426080:CDS
GTTCTTTATCAACAACGCCA+CGG 0.668044 tig0025942:-28002 None:intergenic
CAAGGGAGCTTGAAACCATG+CGG 0.673697 tig0025942:+26695 Msa1426080:CDS
GAGCTGGATAGAACGATGGA+TGG 0.675083 tig0025942:+27960 Msa1426080:CDS
GGTGGAGCTGGATAGAACGA+TGG 0.677630 tig0025942:+27956 Msa1426080:CDS
GCTCTTACAGAAGCACGCAA+GGG 0.686718 tig0025942:+26678 Msa1426080:CDS
GAGCTAACGTCTGCATTGAG+CGG 0.689445 tig0025942:-26588 None:intergenic
GCAGAGTTACCTGTGAGCAA+TGG 0.694985 tig0025942:+28552 Msa1426080:CDS
TCTGTGTTTCATCAACAAGG+AGG 0.705680 tig0025942:+28887 Msa1426080:three_prime_UTR
CTGTATCGATTTCTACAGAG+GGG 0.722657 tig0025942:-26015 None:intergenic
GTTACCTGTGAGCAATGGTG+TGG 0.726390 tig0025942:+28557 Msa1426080:CDS
TGTTGCTGAAATTACAACTG+TGG 0.743093 tig0025942:+26105 Msa1426080:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATAATCAATTTTTTCAA+TGG - tig0025942:25624-25643 None:intergenic 10.0%
!!! AAAATATTTTCAAACTAAAT+TGG - tig0025942:27370-27389 None:intergenic 10.0%
!! AAAATACAAATTGATTAATA+TGG - tig0025942:28776-28795 None:intergenic 10.0%
!!! GCATTTTAAAAAAATGTTAA+AGG - tig0025942:26735-26754 None:intergenic 15.0%
!!! TGAATTTTTTTTAGATGAAT+TGG + tig0025942:26997-27016 Msa1426080:intron 15.0%
!!! TTAAACATTTGTTATAGAAA+GGG - tig0025942:27502-27521 None:intergenic 15.0%
!!! TTTAAACATTTGTTATAGAA+AGG - tig0025942:27503-27522 None:intergenic 15.0%
!! CAATTCATTCAAATTATAAT+AGG - tig0025942:27816-27835 None:intergenic 15.0%
!!! AAAAGTATTAATGAAATGTT+TGG - tig0025942:28726-28745 None:intergenic 15.0%
!!! TTTCATTAATACTTTTGATT+TGG + tig0025942:28730-28749 Msa1426080:three_prime_UTR 15.0%
!!! TTCATTAATACTTTTGATTT+GGG + tig0025942:28731-28750 Msa1426080:three_prime_UTR 15.0%
!! TTTATGAAAAAAAGAGAAAA+AGG - tig0025942:28934-28953 None:intergenic 15.0%
!! ATTCAATCTAAATCAAAGTT+GGG - tig0025942:25508-25527 None:intergenic 20.0%
!! AATTCAATCTAAATCAAAGT+TGG - tig0025942:25509-25528 None:intergenic 20.0%
!! TAATACATACTACTACTAAT+AGG + tig0025942:25532-25551 Msa1426080:intron 20.0%
!! AATACATACTACTACTAATA+GGG + tig0025942:25533-25552 Msa1426080:intron 20.0%
!! TTAAAACATGCTAATATCAA+TGG + tig0025942:25662-25681 Msa1426080:intron 20.0%
!! TGATATAAAGGATCAAATTA+AGG + tig0025942:25774-25793 Msa1426080:intron 20.0%
!! CAAGTAAATCAAAAAAGAAA+TGG + tig0025942:25963-25982 Msa1426080:CDS 20.0%
!! AAATCAAAAAAGAAATGGTT+TGG + tig0025942:25968-25987 Msa1426080:CDS 20.0%
!! AATCAAAAAAGAAATGGTTT+GGG + tig0025942:25969-25988 Msa1426080:CDS 20.0%
!! TCAGTTAATTTAATATCTTC+TGG - tig0025942:26058-26077 None:intergenic 20.0%
!!! TATCATTTTGAAATACCTTT+CGG + tig0025942:26446-26465 Msa1426080:intron 20.0%
!!! ACTGTCTTTTAAAATCATTT+AGG + tig0025942:26474-26493 Msa1426080:intron 20.0%
!!! AATTTTTATTTTCCGATCAA+TGG - tig0025942:27153-27172 None:intergenic 20.0%
!!! CTAAATTGGTTATAAGTTTT+AGG - tig0025942:27356-27375 None:intergenic 20.0%
!! CTATATAGATCTTCAAAAAT+AGG - tig0025942:27448-27467 None:intergenic 20.0%
!! AACTGAATTAAGCATATTAT+TGG - tig0025942:27717-27736 None:intergenic 20.0%
!! CTGATTATTATATAATCAAC+TGG - tig0025942:28583-28602 None:intergenic 20.0%
!! TATATAATAATCAGAGTGAA+AGG + tig0025942:28589-28608 Msa1426080:three_prime_UTR 20.0%
!!! TCATTAATACTTTTGATTTG+GGG + tig0025942:28732-28751 Msa1426080:three_prime_UTR 20.0%
! TTTACAGGAACATGTATTAA+AGG + tig0025942:25291-25310 Msa1426080:five_prime_UTR 25.0%
!!! CTTTTTGTTTAAACACGAAT+TGG + tig0025942:25357-25376 Msa1426080:five_prime_UTR 25.0%
! AAATTTATCTCTCTTGAAGA+AGG - tig0025942:25428-25447 None:intergenic 25.0%
!! TCCTTCTTTTTGTGATATAA+AGG + tig0025942:25762-25781 Msa1426080:intron 25.0%
! TCCTTTATATCACAAAAAGA+AGG - tig0025942:25766-25785 None:intergenic 25.0%
! GTTAATTTAATATCTTCTGG+CGG - tig0025942:26055-26074 None:intergenic 25.0%
! TCTATATATGCAGAATAATG+AGG + tig0025942:26385-26404 Msa1426080:intron 25.0%
!! TTATTTGACTTCGTTTGTAA+TGG + tig0025942:26780-26799 Msa1426080:intron 25.0%
! CCAATCTATAATGAAAATTC+GGG - tig0025942:26818-26837 None:intergenic 25.0%
! CCCAATCTATAATGAAAATT+CGG - tig0025942:26819-26838 None:intergenic 25.0%
!! CCGAATTTTCATTATAGATT+GGG + tig0025942:26816-26835 Msa1426080:intron 25.0%
!! CGAATTTTCATTATAGATTG+GGG + tig0025942:26817-26836 Msa1426080:intron 25.0%
!! CAATTTGTTCTTTAGATTGT+AGG - tig0025942:26859-26878 None:intergenic 25.0%
! TTTGAAGATCTATATAGAAG+AGG + tig0025942:27451-27470 Msa1426080:intron 25.0%
! ATGACAACTGAAAATAACTT+AGG + tig0025942:27573-27592 Msa1426080:intron 25.0%
! ATTATGCAAAAAAATCAACG+TGG - tig0025942:27686-27705 None:intergenic 25.0%
!! TTTTTGCCAATAGAGATATT+TGG + tig0025942:27758-27777 Msa1426080:intron 25.0%
! GTTTGGATACACATAAAAAT+AGG - tig0025942:28709-28728 None:intergenic 25.0%
AATACATGTTCCTGTAAAGA+GGG - tig0025942:25289-25308 None:intergenic 30.0%
TAATACATGTTCCTGTAAAG+AGG - tig0025942:25290-25309 None:intergenic 30.0%
!!! TTAGCATGTTTTAATCTGTG+TGG - tig0025942:25656-25675 None:intergenic 30.0%
! ATTTTCATACCTTTGAGTCA+GGG - tig0025942:25862-25881 None:intergenic 30.0%
! TATTTTCATACCTTTGAGTC+AGG - tig0025942:25863-25882 None:intergenic 30.0%
TTGTAATTTCAGCAACATGA+TGG - tig0025942:26102-26121 None:intergenic 30.0%
! TCAAGATTCAAACAGCTTTT+CGG + tig0025942:26233-26252 Msa1426080:CDS 30.0%
GCGAACAAATTTGAAAATGA+GGG - tig0025942:26286-26305 None:intergenic 30.0%
AGCGAACAAATTTGAAAATG+AGG - tig0025942:26287-26306 None:intergenic 30.0%
AGATTGAAAACATTGATGGA+AGG + tig0025942:26532-26551 Msa1426080:CDS 30.0%
GATTGAAAACATTGATGGAA+GGG + tig0025942:26533-26552 Msa1426080:CDS 30.0%
AAATGCTAATCTCTCAACAT+TGG + tig0025942:26749-26768 Msa1426080:intron 30.0%
! CCCGAATTTTCATTATAGAT+TGG + tig0025942:26815-26834 Msa1426080:intron 30.0%
! ATTTTCATTATAGATTGGGG+AGG + tig0025942:26820-26839 Msa1426080:intron 30.0%
TAGGAAACAAAAGTGAAGAA+AGG - tig0025942:27429-27448 None:intergenic 30.0%
CACTATCCAAATATCTCTAT+TGG - tig0025942:27767-27786 None:intergenic 30.0%
CAATAGAGATATTTGGATAG+TGG + tig0025942:27765-27784 Msa1426080:intron 30.0%
ATAGGACATCACATAATATG+CGG - tig0025942:27798-27817 None:intergenic 30.0%
ATCTATGAATCCAATGTTTG+TGG + tig0025942:27912-27931 Msa1426080:CDS 30.0%
AGTCCATTAGCAAAAGAAAA+TGG + tig0025942:28417-28436 Msa1426080:CDS 30.0%
!!! TTGCCATTTTCTTTTGCTAA+TGG - tig0025942:28423-28442 None:intergenic 30.0%
AGAAAATGGCAAACAAGAAA+AGG + tig0025942:28431-28450 Msa1426080:CDS 30.0%
GAAAATGGCAAACAAGAAAA+GGG + tig0025942:28432-28451 Msa1426080:CDS 30.0%
AAAATGGCAAACAAGAAAAG+GGG + tig0025942:28433-28452 Msa1426080:CDS 30.0%
AGGACAGAAATCATTAAAGT+AGG + tig0025942:28609-28628 Msa1426080:three_prime_UTR 30.0%
GAAACTCTTGATTCTAAATG+TGG + tig0025942:28963-28982 Msa1426080:three_prime_UTR 30.0%
! TCTGATTTTTCCCTCTTTAC+AGG + tig0025942:25276-25295 Msa1426080:five_prime_UTR 35.0%
AGGAACATGTATTAAAGGTG+TGG + tig0025942:25296-25315 Msa1426080:five_prime_UTR 35.0%
! CATGTATTAAAGGTGTGGTT+TGG + tig0025942:25301-25320 Msa1426080:five_prime_UTR 35.0%
!! TATTAAAGGTGTGGTTTGGT+TGG + tig0025942:25305-25324 Msa1426080:five_prime_UTR 35.0%
TCAGATCTTTCACTTCATTC+AGG + tig0025942:25386-25405 Msa1426080:five_prime_UTR 35.0%
CATACTACTACTAATAGGGA+AGG + tig0025942:25537-25556 Msa1426080:intron 35.0%
ACTACTAATAGGGAAGGATT+AGG + tig0025942:25543-25562 Msa1426080:intron 35.0%
AAGGAATGCCAAGAAGATAA+TGG + tig0025942:25793-25812 Msa1426080:exon 35.0%
AGGAATGCCAAGAAGATAAT+GGG + tig0025942:25794-25813 Msa1426080:exon 35.0%
!! AAGAAGATAATGGGGAAGAA+GGG + tig0025942:25803-25822 Msa1426080:exon 35.0%
AATTTAATATCTTCTGGCGG+AGG - tig0025942:26052-26071 None:intergenic 35.0%
TGTTGCTGAAATTACAACTG+TGG + tig0025942:26105-26124 Msa1426080:CDS 35.0%
! TCATTTTGCTGGTAAACCAA+AGG + tig0025942:26195-26214 Msa1426080:CDS 35.0%
! CAAGATTCAAACAGCTTTTC+GGG + tig0025942:26234-26253 Msa1426080:CDS 35.0%
! AAGATTCAAACAGCTTTTCG+GGG + tig0025942:26235-26254 Msa1426080:CDS 35.0%
!! CAGAATAATGAGGCTTTAAG+AGG + tig0025942:26395-26414 Msa1426080:intron 35.0%
AAAAGACAGTTTAGTCCGAA+AGG - tig0025942:26464-26483 None:intergenic 35.0%
GGTGAGATTGAAAACATTGA+TGG + tig0025942:26528-26547 Msa1426080:CDS 35.0%
AAACGAAGTCAAATAAGCGT+TGG - tig0025942:26776-26795 None:intergenic 35.0%
ATTATAGATTGGGGAGGAAT+GGG + tig0025942:26826-26845 Msa1426080:intron 35.0%
CTCATACTTGCTCAGTAATT+TGG - tig0025942:26885-26904 None:intergenic 35.0%
CAAATTACTGAGCAAGTATG+AGG + tig0025942:26883-26902 Msa1426080:CDS 35.0%
CTTGTTAACTTAGATATGCG+AGG + tig0025942:26966-26985 Msa1426080:intron 35.0%
CAGAACATAACATGAAAGCT+TGG - tig0025942:27074-27093 None:intergenic 35.0%
CAAGCTTTCATGTTATGTTC+TGG + tig0025942:27072-27091 Msa1426080:intron 35.0%
ATATGTCAACGACCATTGAT+CGG + tig0025942:27138-27157 Msa1426080:intron 35.0%
AACTGCTGATGAGAAAATCA+CGG - tig0025942:27236-27255 None:intergenic 35.0%
CAACAACTTCAAGTAGGATA+AGG - tig0025942:27324-27343 None:intergenic 35.0%
AAAGAGCAACAACTTCAAGT+AGG - tig0025942:27330-27349 None:intergenic 35.0%
! GTGAAGAAAGGTGACAATTT+TGG - tig0025942:27417-27436 None:intergenic 35.0%
TAGAAGAGGATGAAAACAAG+AGG + tig0025942:27465-27484 Msa1426080:intron 35.0%
AGAAGAGGATGAAAACAAGA+GGG + tig0025942:27466-27485 Msa1426080:intron 35.0%
GAAGATAGTGACTACATGAA+AGG - tig0025942:27617-27636 None:intergenic 35.0%
AAAAAAATCAACGTGGCAGT+GGG - tig0025942:27679-27698 None:intergenic 35.0%
AATGTATTTCAGAGTTGAGC+TGG - tig0025942:28104-28123 None:intergenic 35.0%
TCCATCAGTTCCAAGTTATA+TGG + tig0025942:28350-28369 Msa1426080:CDS 35.0%
ACCATATAACTTGGAACTGA+TGG - tig0025942:28354-28373 None:intergenic 35.0%
ATTCTTTACCATTGACCTTG+AGG + tig0025942:28803-28822 Msa1426080:three_prime_UTR 35.0%
TTCTTTACCATTGACCTTGA+GGG + tig0025942:28804-28823 Msa1426080:three_prime_UTR 35.0%
! CTTGTTGATGAAACACAGAA+TGG - tig0025942:28886-28905 None:intergenic 35.0%
CATTCTGTGTTTCATCAACA+AGG + tig0025942:28884-28903 Msa1426080:three_prime_UTR 35.0%
! TCTCTTGAAGAAGGTGCAAT+GGG - tig0025942:25419-25438 None:intergenic 40.0%
GGAAGGATTAGGAGTTTGAA+TGG + tig0025942:25554-25573 Msa1426080:intron 40.0%
GGAATGCCAAGAAGATAATG+GGG + tig0025942:25795-25814 Msa1426080:exon 40.0%
CTTCTTCCCCATTATCTTCT+TGG - tig0025942:25804-25823 None:intergenic 40.0%
!! CAAGAAGATAATGGGGAAGA+AGG + tig0025942:25802-25821 Msa1426080:exon 40.0%
TAATGGGGAAGAAGGGAAAT+TGG + tig0025942:25810-25829 Msa1426080:exon 40.0%
!! CTGTATCGATTTCTACAGAG+GGG - tig0025942:26018-26037 None:intergenic 40.0%
!! GCTGTATCGATTTCTACAGA+GGG - tig0025942:26019-26038 None:intergenic 40.0%
!! TGCTGTATCGATTTCTACAG+AGG - tig0025942:26020-26039 None:intergenic 40.0%
TGCTGAAATTACAACTGTGG+TGG + tig0025942:26108-26127 Msa1426080:CDS 40.0%
TGGTAAACCAAAGGATGAAG+TGG + tig0025942:26204-26223 Msa1426080:CDS 40.0%
!! TTTAGGCAAGAAGAGCATTG+CGG + tig0025942:26491-26510 Msa1426080:intron 40.0%
!! ATGGCTTGGCGTTTTACAAT+CGG - tig0025942:26559-26578 None:intergenic 40.0%
TGCAATCTCAGATTCGTTCT+AGG + tig0025942:26614-26633 Msa1426080:CDS 40.0%
! AATCTCAGATTCGTTCTAGG+AGG + tig0025942:26617-26636 Msa1426080:CDS 40.0%
!! ATCTCAGATTCGTTCTAGGA+GGG + tig0025942:26618-26637 Msa1426080:CDS 40.0%
CATTATAGATTGGGGAGGAA+TGG + tig0025942:26825-26844 Msa1426080:intron 40.0%
ACTGCTGATGAGAAAATCAC+GGG - tig0025942:27235-27254 None:intergenic 40.0%
GAAGAGGATGAAAACAAGAG+GGG + tig0025942:27467-27486 Msa1426080:intron 40.0%
AAGAGGATGAAAACAAGAGG+GGG + tig0025942:27468-27487 Msa1426080:intron 40.0%
AGAGGTTTAAGCAGAGGAAA+TGG - tig0025942:27644-27663 None:intergenic 40.0%
CAAAAAAATCAACGTGGCAG+TGG - tig0025942:27680-27699 None:intergenic 40.0%
GTTCTTTATCAACAACGCCA+CGG - tig0025942:28005-28024 None:intergenic 40.0%
TTTCTCCACCTATAATGCTG+CGG - tig0025942:28059-28078 None:intergenic 40.0%
TTTCAGAGTTGAGCTGGAAA+CGG - tig0025942:28098-28117 None:intergenic 40.0%
GTTGAATTGGACTGAAAGCT+GGG - tig0025942:28156-28175 None:intergenic 40.0%
AGTTGAATTGGACTGAAAGC+TGG - tig0025942:28157-28176 None:intergenic 40.0%
CAATTCAACTCCTACTAAGC+CGG + tig0025942:28167-28186 Msa1426080:CDS 40.0%
AATTCAACTCCTACTAAGCC+GGG + tig0025942:28168-28187 Msa1426080:CDS 40.0%
AGTTGCTAGGAAACTGAAGA+AGG + tig0025942:28200-28219 Msa1426080:CDS 40.0%
ATCATCTCTAACTGATGACC+CGG - tig0025942:28313-28332 None:intergenic 40.0%
AAACAAGAAAAGGGGTCCTT+TGG + tig0025942:28441-28460 Msa1426080:CDS 40.0%
TTTATGGCGGTTTCTACCTT+CGG - tig0025942:28531-28550 None:intergenic 40.0%
AGGTAACTCTGCATTTATGG+CGG - tig0025942:28544-28563 None:intergenic 40.0%
CACAGGTAACTCTGCATTTA+TGG - tig0025942:28547-28566 None:intergenic 40.0%
TCTTTACCATTGACCTTGAG+GGG + tig0025942:28805-28824 Msa1426080:three_prime_UTR 40.0%
AACCAAATTTCTTCCCCTCA+AGG - tig0025942:28821-28840 None:intergenic 40.0%
GGTTCATGTTGATCTTGTGA+CGG + tig0025942:28837-28856 Msa1426080:three_prime_UTR 40.0%
GTTCATGTTGATCTTGTGAC+GGG + tig0025942:28838-28857 Msa1426080:three_prime_UTR 40.0%
TCTGTGTTTCATCAACAAGG+AGG + tig0025942:28887-28906 Msa1426080:three_prime_UTR 40.0%
! CTCTCTTGAAGAAGGTGCAA+TGG - tig0025942:25420-25439 None:intergenic 45.0%
! GCTGCAGTTTCTCATTTTGC+TGG + tig0025942:26184-26203 Msa1426080:CDS 45.0%
! ATTGCTGCCACTTCATCCTT+TGG - tig0025942:26214-26233 None:intergenic 45.0%
!! AACAGCTTTTCGGGGATACT+TGG + tig0025942:26243-26262 Msa1426080:CDS 45.0%
!! TTAGGCAAGAAGAGCATTGC+GGG + tig0025942:26492-26511 Msa1426080:intron 45.0%
TAGGAGGGTCAGAATGTTAG+AGG + tig0025942:26633-26652 Msa1426080:CDS 45.0%
CAGAATGTTAGAGGAGGATC+AGG + tig0025942:26642-26661 Msa1426080:CDS 45.0%
! TATGCGAAGAGAAAGAGCAC+TGG + tig0025942:26910-26929 Msa1426080:CDS 45.0%
GGCTTATGCATTCACTCATC+AGG + tig0025942:26931-26950 Msa1426080:CDS 45.0%
TTGGTGGGATCCACAAACAT+TGG - tig0025942:27925-27944 None:intergenic 45.0%
TTCAGAGTTGAGCTGGAAAC+GGG - tig0025942:28097-28116 None:intergenic 45.0%
CGGCTTAGTAGGAGTTGAAT+TGG - tig0025942:28169-28188 None:intergenic 45.0%
TCCAAAGGGAAGTTGGGTTA+TGG + tig0025942:28227-28246 Msa1426080:CDS 45.0%
TCCATAACCCAACTTCCCTT+TGG - tig0025942:28231-28250 None:intergenic 45.0%
GGATGATGACTCCAAAAGCA+TGG + tig0025942:28248-28267 Msa1426080:CDS 45.0%
! CTGTACACTTGCCATGCTTT+TGG - tig0025942:28262-28281 None:intergenic 45.0%
TGGCAAGTGTACAGTCAGAT+CGG + tig0025942:28268-28287 Msa1426080:CDS 45.0%
TGAGTCGGCACCATATAACT+TGG - tig0025942:28363-28382 None:intergenic 45.0%
! CTAGCTTTCGCAGATTGAGT+CGG - tig0025942:28378-28397 None:intergenic 45.0%
!! GAAAAAGCGCCTTTCTTACC+CGG + tig0025942:28470-28489 Msa1426080:CDS 45.0%
TTTCTTCCCCTCAAGGTCAA+TGG - tig0025942:28814-28833 None:intergenic 45.0%
GACCTTGAGGGGAAGAAATT+TGG + tig0025942:28816-28835 Msa1426080:three_prime_UTR 45.0%
!!! ATTTTAATTATAATTACAAA+AGG - tig0025942:27547-27566 None:intergenic 5.0%
ATATCTTCTGGCGGAGGAAG+AGG - tig0025942:26046-26065 None:intergenic 50.0%
!! TACAACTGTGGTGGATGTCG+AGG + tig0025942:26117-26136 Msa1426080:CDS 50.0%
GCTGTCTGAACAGAACGAAC+AGG - tig0025942:26145-26164 None:intergenic 50.0%
!! AGAGCATTGCGGGCTTTAAG+AGG + tig0025942:26502-26521 Msa1426080:CDS 50.0%
!! GAGCATTGCGGGCTTTAAGA+GGG + tig0025942:26503-26522 Msa1426080:CDS 50.0%
TAACGTCTGCATTGAGCGGA+GGG - tig0025942:26587-26606 None:intergenic 50.0%
GAGCTAACGTCTGCATTGAG+CGG - tig0025942:26591-26610 None:intergenic 50.0%
GAGGGTCAGAATGTTAGAGG+AGG + tig0025942:26636-26655 Msa1426080:CDS 50.0%
! AGCTCTTACAGAAGCACGCA+AGG + tig0025942:26677-26696 Msa1426080:CDS 50.0%
! GCTCTTACAGAAGCACGCAA+GGG + tig0025942:26678-26697 Msa1426080:CDS 50.0%
CAAGGGAGCTTGAAACCATG+CGG + tig0025942:26695-26714 Msa1426080:CDS 50.0%
AAGGGAGCTTGAAACCATGC+GGG + tig0025942:26696-26715 Msa1426080:CDS 50.0%
AGCTTGAAACCATGCGGGTA+TGG + tig0025942:26701-26720 Msa1426080:intron 50.0%
GCTTGAAACCATGCGGGTAT+GGG + tig0025942:26702-26721 Msa1426080:intron 50.0%
GAAAGAAACCCATACCCGCA+TGG - tig0025942:26713-26732 None:intergenic 50.0%
GGTTGCAGAGGTTTAAGCAG+AGG - tig0025942:27650-27669 None:intergenic 50.0%
AATCAACGTGGCAGTGGGAA+AGG - tig0025942:27674-27693 None:intergenic 50.0%
TGGATCCCACCAATCCTACT+TGG + tig0025942:27932-27951 Msa1426080:CDS 50.0%
GGATCCCACCAATCCTACTT+GGG + tig0025942:27933-27952 Msa1426080:CDS 50.0%
GATCCCACCAATCCTACTTG+GGG + tig0025942:27934-27953 Msa1426080:CDS 50.0%
ATCCCACCAATCCTACTTGG+GGG + tig0025942:27935-27954 Msa1426080:CDS 50.0%
GAGCTGGATAGAACGATGGA+TGG + tig0025942:27960-27979 Msa1426080:CDS 50.0%
AGTTCGAGCCGCAGCATTAT+AGG + tig0025942:28048-28067 Msa1426080:CDS 50.0%
CTAGCAACTGAAGCAGAACC+CGG - tig0025942:28189-28208 None:intergenic 50.0%
ACTGAAGAAGGCGAGTCCAA+AGG + tig0025942:28212-28231 Msa1426080:CDS 50.0%
CTGAAGAAGGCGAGTCCAAA+GGG + tig0025942:28213-28232 Msa1426080:CDS 50.0%
CGACTCAATCTGCGAAAGCT+AGG + tig0025942:28376-28395 Msa1426080:CDS 50.0%
!!! GCGCTTTTTCGCAGTTCCAA+AGG - tig0025942:28460-28479 None:intergenic 50.0%
GCAGAGTTACCTGTGAGCAA+TGG + tig0025942:28552-28571 Msa1426080:CDS 50.0%
GTTACCTGTGAGCAATGGTG+TGG + tig0025942:28557-28576 Msa1426080:CDS 50.0%
! CATGTTGATCTTGTGACGGG+AGG + tig0025942:28841-28860 Msa1426080:three_prime_UTR 50.0%
AGCTCTCAGCCCTGACTCAA+AGG + tig0025942:25850-25869 Msa1426080:CDS 55.0%
!! ATTGCGGGCTTTAAGAGGGC+TGG + tig0025942:26507-26526 Msa1426080:CDS 55.0%
!! CATTGAGCGGAGGGTACTCA+TGG - tig0025942:26578-26597 None:intergenic 55.0%
CTAACGTCTGCATTGAGCGG+AGG - tig0025942:26588-26607 None:intergenic 55.0%
AGTGGGAAAGGAGGTTGCAG+AGG - tig0025942:27662-27681 None:intergenic 55.0%
! CACCCCCAAGTAGGATTGGT+GGG - tig0025942:27940-27959 None:intergenic 55.0%
GGTGGAGCTGGATAGAACGA+TGG + tig0025942:27956-27975 Msa1426080:CDS 55.0%
TCGAGCCGCAGCATTATAGG+TGG + tig0025942:28051-28070 Msa1426080:CDS 55.0%
!!! CCAGGATTTTGGCTCGCTAC+TGG - tig0025942:28129-28148 None:intergenic 55.0%
CCAGTAGCGAGCCAAAATCC+TGG + tig0025942:28126-28145 Msa1426080:CDS 55.0%
! AGCTGGGTGAGCCAGGATTT+TGG - tig0025942:28140-28159 None:intergenic 55.0%
GAAGCAGAACCCGGCTTAGT+AGG - tig0025942:28180-28199 None:intergenic 55.0%
CGGGTTCTGCTTCAGTTGCT+AGG + tig0025942:28187-28206 Msa1426080:CDS 55.0%
AGGCGAGTCCAAAGGGAAGT+TGG + tig0025942:28220-28239 Msa1426080:CDS 55.0%
GGCGAGTCCAAAGGGAAGTT+GGG + tig0025942:28221-28240 Msa1426080:CDS 55.0%
! TACAGTCAGATCGGTTCCGC+AGG + tig0025942:28277-28296 Msa1426080:CDS 55.0%
TTCCGCAGGCACTCTATTGC+CGG + tig0025942:28291-28310 Msa1426080:CDS 55.0%
ATGGCGGTTTCTACCTTCGG+TGG - tig0025942:28528-28547 None:intergenic 55.0%
TCTTCTGGCGGAGGAAGAGG+CGG - tig0025942:26043-26062 None:intergenic 60.0%
!! AGCGGAGGGTACTCATGGCT+TGG - tig0025942:26573-26592 None:intergenic 60.0%
CAACGTGGCAGTGGGAAAGG+AGG - tig0025942:27671-27690 None:intergenic 60.0%
CCACCCCCAAGTAGGATTGG+TGG - tig0025942:27941-27960 None:intergenic 60.0%
CCACCAATCCTACTTGGGGG+TGG + tig0025942:27938-27957 Msa1426080:CDS 60.0%
GCTCCACCCCCAAGTAGGAT+TGG - tig0025942:27944-27963 None:intergenic 60.0%
ATCCTACTTGGGGGTGGAGC+TGG + tig0025942:27944-27963 Msa1426080:CDS 60.0%
ATCCAGCTCCACCCCCAAGT+AGG - tig0025942:27949-27968 None:intergenic 60.0%
ATGGATGGCTGCCCGACCTT+GGG + tig0025942:27975-27994 Msa1426080:CDS 60.0%
GACTGAAAGCTGGGTGAGCC+AGG - tig0025942:28147-28166 None:intergenic 60.0%
TCCGCAGGCACTCTATTGCC+GGG + tig0025942:28292-28311 Msa1426080:CDS 60.0%
! ACCCGGCAATAGAGTGCCTG+CGG - tig0025942:28296-28315 None:intergenic 60.0%
CAGGCGAAGCCGGGTAAGAA+AGG - tig0025942:28482-28501 None:intergenic 60.0%
GGTGGACCTGAATGACGCCT+TGG - tig0025942:28510-28529 None:intergenic 60.0%
GCGTCATTCAGGTCCACCGA+AGG + tig0025942:28512-28531 Msa1426080:CDS 60.0%
CTGGCCACACCATTGCTCAC+AGG - tig0025942:28564-28583 None:intergenic 60.0%
GATGGATGGCTGCCCGACCT+TGG + tig0025942:27974-27993 Msa1426080:CDS 65.0%
ACAACGCCACGGCTCTCCCA+AGG - tig0025942:27994-28013 None:intergenic 65.0%
GAATGACGCCTTGGCCTCGC+AGG - tig0025942:28501-28520 None:intergenic 65.0%
! GCGAGGCCAAGGCGTCATTC+AGG + tig0025942:28501-28520 Msa1426080:CDS 65.0%
GCCACGGCTCTCCCAAGGTC+GGG - tig0025942:27989-28008 None:intergenic 70.0%
CGCCACGGCTCTCCCAAGGT+CGG - tig0025942:27990-28009 None:intergenic 70.0%
CTTACCCGGCTTCGCCTGCG+AGG + tig0025942:28484-28503 Msa1426080:CDS 70.0%
TTGGCCTCGCAGGCGAAGCC+GGG - tig0025942:28491-28510 None:intergenic 70.0%
CTTGGCCTCGCAGGCGAAGC+CGG - tig0025942:28492-28511 None:intergenic 70.0%
GCCCGACCTTGGGAGAGCCG+TGG + tig0025942:27985-28004 Msa1426080:CDS 75.0%
CGGCTTCGCCTGCGAGGCCA+AGG + tig0025942:28490-28509 Msa1426080:CDS 75.0%
Chromosome Type Strat End Strand Name
tig0025942 gene 25234 29080 25234 ID=Msa1426080;Name=Msa1426080
tig0025942 mRNA 25234 29080 25234 ID=Msa1426080-mRNA-1;Parent=Msa1426080;Name=Msa1426080-mRNA-1;_AED=0.06;_eAED=0.06;_QI=206|1|1|1|0.8|0.83|6|493|468
tig0025942 exon 25234 25407 25234 ID=Msa1426080-mRNA-1:exon:4667;Parent=Msa1426080-mRNA-1
tig0025942 exon 25780 25871 25780 ID=Msa1426080-mRNA-1:exon:4668;Parent=Msa1426080-mRNA-1
tig0025942 exon 25959 26264 25959 ID=Msa1426080-mRNA-1:exon:4669;Parent=Msa1426080-mRNA-1
tig0025942 exon 26496 26717 26496 ID=Msa1426080-mRNA-1:exon:4670;Parent=Msa1426080-mRNA-1
tig0025942 exon 26833 26952 26833 ID=Msa1426080-mRNA-1:exon:4671;Parent=Msa1426080-mRNA-1
tig0025942 exon 27889 29080 27889 ID=Msa1426080-mRNA-1:exon:4672;Parent=Msa1426080-mRNA-1
tig0025942 five_prime_UTR 25234 25407 25234 ID=Msa1426080-mRNA-1:five_prime_utr;Parent=Msa1426080-mRNA-1
tig0025942 five_prime_UTR 25780 25811 25780 ID=Msa1426080-mRNA-1:five_prime_utr;Parent=Msa1426080-mRNA-1
tig0025942 CDS 25812 25871 25812 ID=Msa1426080-mRNA-1:cds;Parent=Msa1426080-mRNA-1
tig0025942 CDS 25959 26264 25959 ID=Msa1426080-mRNA-1:cds;Parent=Msa1426080-mRNA-1
tig0025942 CDS 26496 26717 26496 ID=Msa1426080-mRNA-1:cds;Parent=Msa1426080-mRNA-1
tig0025942 CDS 26833 26952 26833 ID=Msa1426080-mRNA-1:cds;Parent=Msa1426080-mRNA-1
tig0025942 CDS 27889 28587 27889 ID=Msa1426080-mRNA-1:cds;Parent=Msa1426080-mRNA-1
tig0025942 three_prime_UTR 28588 29080 28588 ID=Msa1426080-mRNA-1:three_prime_utr;Parent=Msa1426080-mRNA-1
Gene Sequence

>Msa1426080

ATGGGGAAGAAGGGAAATTGGTTTTCTACAGTGAAGAAAGCTCTCAGCCCTGACTCAAAGAAATCAAGTAAATCAAAAAAGAAATGGTTTGGGAAGCAAAAATTGCAGACTTCAGACCCCTCTGTAGAAATCGATACAGCACCGCCTCTTCCTCCGCCAGAAGATATTAAATTAACTGATATTGAAAATCAAAACAATCACCATCATGTTGCTGAAATTACAACTGTGGTGGATGTCGAGGAACCTGTTCGTTCTGTTCAGACAGCAGTTGTTAAGACTCAAGCTGCTGCAGTTTCTCATTTTGCTGGTAAACCAAAGGATGAAGTGGCAGCAATCAAGATTCAAACAGCTTTTCGGGGATACTTGGCAAGAAGAGCATTGCGGGCTTTAAGAGGGCTGGTGAGATTGAAAACATTGATGGAAGGGCCGATTGTAAAACGCCAAGCCATGAGTACCCTCCGCTCAATGCAGACGTTAGCTCGTGTGCAATCTCAGATTCGTTCTAGGAGGGTCAGAATGTTAGAGGAGGATCAGGCTTTACAGAGACAGCTCTTACAGAAGCACGCAAGGGAGCTTGAAACCATGCGGATTGGGGAGGAATGGGACGATAGCCTACAATCTAAAGAACAAATTGAAGCCAAATTACTGAGCAAGTATGAGGCTACTATGCGAAGAGAAAGAGCACTGGCTTATGCATTCACTCATCAGCAAAACTCGAAGAACTCGTCTAGATCTATGAATCCAATGTTTGTGGATCCCACCAATCCTACTTGGGGGTGGAGCTGGATAGAACGATGGATGGCTGCCCGACCTTGGGAGAGCCGTGGCGTTGTTGATAAAGAACTGAATGATCATTCATCTGTTAAAAGTTCGAGCCGCAGCATTATAGGTGGAGAAATCAGCAAATCATTTGCCCGTTTCCAGCTCAACTCTGAAATACATTCTCCAGTAGCGAGCCAAAATCCTGGCTCACCCAGCTTTCAGTCCAATTCAACTCCTACTAAGCCGGGTTCTGCTTCAGTTGCTAGGAAACTGAAGAAGGCGAGTCCAAAGGGAAGTTGGGTTATGGATGATGACTCCAAAAGCATGGCAAGTGTACAGTCAGATCGGTTCCGCAGGCACTCTATTGCCGGGTCATCAGTTAGAGATGATGAGAGTCTTGCTAGCTCTCCATCAGTTCCAAGTTATATGGTGCCGACTCAATCTGCGAAAGCTAGGTTGAGAACACAAAGTCAAAGTCCATTAGCAAAAGAAAATGGCAAACAAGAAAAGGGGTCCTTTGGAACTGCGAAAAAGCGCCTTTCTTACCCGGCTTCGCCTGCGAGGCCAAGGCGTCATTCAGGTCCACCGAAGGTAGAAACCGCCATAAATGCAGAGTTACCTGTGAGCAATGGTGTGGCCAGTTGA
Protein sequence

>Msa1426080

MGKKGNWFSTVKKALSPDSKKSSKSKKKWFGKQKLQTSDPSVEIDTAPPLPPPEDIKLTDIENQNNHHHVAEITTVVDVEEPVRSVQTAVVKTQAAAVSHFAGKPKDEVAAIKIQTAFRGYLARRALRALRGLVRLKTLMEGPIVKRQAMSTLRSMQTLARVQSQIRSRRVRMLEEDQALQRQLLQKHARELETMRIGEEWDDSLQSKEQIEAKLLSKYEATMRRERALAYAFTHQQNSKNSSRSMNPMFVDPTNPTWGWSWIERWMAARPWESRGVVDKELNDHSSVKSSSRSIIGGEISKSFARFQLNSEIHSPVASQNPGSPSFQSNSTPTKPGSASVARKLKKASPKGSWVMDDDSKSMASVQSDRFRRHSIAGSSVRDDESLASSPSVPSYMVPTQSAKARLRTQSQSPLAKENGKQEKGSFGTAKKRLSYPASPARPRRHSGPPKVETAINAELPVSNGVAS*