Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1442590 | G7LHP4 | 72.308 | 130 | 16 | 3 | 21 | 141 | 50 | 168 | 6.09e-21 | 93.2 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0191040 | Msa1442590 | 0.807626 | 4.609999e-50 | -8.615850e-47 |
| Msa0206800 | Msa1442590 | 0.806350 | 8.586562e-50 | -8.615850e-47 |
| Msa0233340 | Msa1442590 | 0.807169 | 5.764754e-50 | -8.615850e-47 |
| Msa1033560 | Msa1442590 | 0.800339 | 1.509462e-48 | -8.615850e-47 |
| Msa0273990 | Msa1442590 | 0.801281 | 9.695021e-49 | -8.615850e-47 |
| Msa0314630 | Msa1442590 | 0.808345 | 3.242281e-50 | -8.615850e-47 |
| Msa0326540 | Msa1442590 | 0.800250 | 1.574311e-48 | -8.615850e-47 |
| Msa1113850 | Msa1442590 | 0.800780 | 1.227161e-48 | -8.615850e-47 |
| Msa1169990 | Msa1442590 | 0.813164 | 2.940670e-51 | -8.615850e-47 |
| Msa1217940 | Msa1442590 | 0.808817 | 2.570052e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1442590 | MtrunA17_Chr8g0366631 | 72.308 | 130 | 16 | 3 | 21 | 141 | 50 | 168 | 1.17e-24 | 93.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 70 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGGTGATGGAGGACTTCC+TGG | 0.297879 | tig0030133:+2289 | Msa1442590:CDS |
| CCTCCAGATCCTTCTTCATT+AGG | 0.310213 | tig0030133:-2370 | None:intergenic |
| TGTACCAGGTGAAGGTGGTT+TGG | 0.341135 | tig0030133:+2270 | Msa1442590:CDS |
| GAAGGATCTGGAGGACTTCC+TGG | 0.345249 | tig0030133:+2379 | Msa1442590:CDS |
| GTACCAGGTGAAGGTGGTTT+GGG | 0.359814 | tig0030133:+2271 | Msa1442590:CDS |
| GGAGGACTTCCTGGTGGTAT+AGG | 0.375867 | tig0030133:+2388 | Msa1442590:CDS |
| GGAGTTCTGCCTGATGAAAA+AGG | 0.389577 | tig0030133:+2640 | Msa1442590:CDS |
| TGTACCAGATGAAGGAGATT+TGG | 0.396430 | tig0030133:+2441 | Msa1442590:CDS |
| GGAGTAAGTAGTTTAACAAT+AGG | 0.410712 | tig0030133:+2208 | Msa1442590:CDS |
| CCCGTTGGTGCAGAAGTTCC+TGG | 0.413835 | tig0030133:+2538 | Msa1442590:CDS |
| CTTCCTGGTGGTATAGGATC+TGG | 0.418320 | tig0030133:+2394 | Msa1442590:CDS |
| TATCCTGGTGGTGTGAATTC+CGG | 0.423300 | tig0030133:+2499 | Msa1442590:CDS |
| AGTTTAACAATAGGAGTCAT+AGG | 0.432968 | tig0030133:+2217 | Msa1442590:CDS |
| GGGTTTCCTGTAGGACTGCC+TGG | 0.435618 | tig0030133:+2247 | Msa1442590:CDS |
| GGCAGAACTCCTCTTTCATC+AGG | 0.445935 | tig0030133:-2628 | None:intergenic |
| ACAATAGGAGTCATAGGATC+TGG | 0.448118 | tig0030133:+2223 | Msa1442590:CDS |
| TCTTCCAGACCTCCTTCAAC+TGG | 0.449061 | tig0030133:-2595 | None:intergenic |
| GGTGAAGGTGGTTTGGGTGA+TGG | 0.453512 | tig0030133:+2277 | Msa1442590:CDS |
| CCAGGAACTTCTGCACCAAC+GGG | 0.458513 | tig0030133:-2538 | None:intergenic |
| CTTCCTAATGAAGAAGGATC+TGG | 0.459760 | tig0030133:+2367 | Msa1442590:CDS |
| GATGAAGGACTGCCTGGTAC+TGG | 0.459909 | tig0030133:+2571 | Msa1442590:CDS |
| ATAGGATCTGGAGCACTGCC+TGG | 0.462961 | tig0030133:+2406 | Msa1442590:CDS |
| ACCAGGAACTTCTGCACCAA+CGG | 0.467348 | tig0030133:-2539 | None:intergenic |
| TGGACCAGTTGAAGGAGGTC+TGG | 0.467854 | tig0030133:+2591 | Msa1442590:CDS |
| CCTGGTACTGGACCAGTTGA+AGG | 0.468919 | tig0030133:+2583 | Msa1442590:CDS |
| GTAGGACTGCCTGGTGTACC+AGG | 0.469604 | tig0030133:+2256 | Msa1442590:CDS |
| GTACCAGATGAAGGAGATTT+GGG | 0.475834 | tig0030133:+2442 | Msa1442590:CDS |
| TCACCCAAATCTCCTTCATC+TGG | 0.480841 | tig0030133:-2445 | None:intergenic |
| AAAGTACTTGGAGGACTGCC+TGG | 0.491036 | tig0030133:+2472 | Msa1442590:CDS |
| GTTGAAGGAGGTCTGGAAGA+TGG | 0.497831 | tig0030133:+2598 | Msa1442590:CDS |
| CCTTCAACTGGTCCAGTACC+AGG | 0.498932 | tig0030133:-2583 | None:intergenic |
| CCTGGTGAAGGAGGAGATGA+AGG | 0.499207 | tig0030133:+2556 | Msa1442590:CDS |
| GGTACACCAGGCAGTCCTAC+AGG | 0.504481 | tig0030133:-2253 | None:intergenic |
| GCACTGCCTGGAGGACTTCG+TGG | 0.511832 | tig0030133:+2418 | Msa1442590:CDS |
| GAAGGTGGTTTGGGTGATGG+AGG | 0.518517 | tig0030133:+2280 | Msa1442590:CDS |
| GATCCTTCTTCATTAGGAAG+TGG | 0.519388 | tig0030133:-2364 | None:intergenic |
| GGAGGAGATGAAGGACTGCC+TGG | 0.519861 | tig0030133:+2565 | Msa1442590:CDS |
| GAACTCCTCTTTCATCAGGC+AGG | 0.521690 | tig0030133:-2624 | None:intergenic |
| CCACCTTCACCTGGTACACC+AGG | 0.532618 | tig0030133:-2265 | None:intergenic |
| CCTTCATCTCCTCCTTCACC+AGG | 0.541018 | tig0030133:-2556 | None:intergenic |
| GGACTGCCTGGTGAATATCC+TGG | 0.547709 | tig0030133:+2484 | Msa1442590:CDS |
| ATTCCACTTCCTAATGAAGA+AGG | 0.553225 | tig0030133:+2361 | Msa1442590:CDS |
| GGTACACCACGAAGTCCTCC+AGG | 0.556740 | tig0030133:-2424 | None:intergenic |
| GGAGTCCTGCCTGATGAAAG+AGG | 0.569327 | tig0030133:+2619 | Msa1442590:CDS |
| AAGTGGAATGTGCTCACCCG+TGG | 0.571927 | tig0030133:-2347 | None:intergenic |
| CTTCGTGGTGTACCAGATGA+AGG | 0.572822 | tig0030133:+2433 | Msa1442590:CDS |
| CCACCGGAATTCACACCACC+AGG | 0.574503 | tig0030133:-2502 | None:intergenic |
| GGGTTGATAGCCGTCTCCAC+CGG | 0.580996 | tig0030133:-2518 | None:intergenic |
| TCACCCAAACCACCTTCACC+TGG | 0.587899 | tig0030133:-2274 | None:intergenic |
| GCAAGTTACTTAATGATACA+AGG | 0.589099 | tig0030133:+2187 | None:intergenic |
| GGTGTGAATTCCGGTGGAGA+CGG | 0.598585 | tig0030133:+2508 | Msa1442590:CDS |
| GCAGAAGTTCCTGGTGAAGG+AGG | 0.602305 | tig0030133:+2547 | Msa1442590:CDS |
| GGTGCAGAAGTTCCTGGTGA+AGG | 0.603113 | tig0030133:+2544 | Msa1442590:CDS |
| GGAATAGGCTATATTCCCAC+GGG | 0.610164 | tig0030133:+2331 | Msa1442590:intron |
| GGATCTGGAGGGTTTCCTGT+AGG | 0.614115 | tig0030133:+2238 | Msa1442590:CDS |
| ATAGGAGTCATAGGATCTGG+AGG | 0.620341 | tig0030133:+2226 | Msa1442590:CDS |
| TGGAATAGGCTATATTCCCA+CGG | 0.628199 | tig0030133:+2330 | Msa1442590:intron |
| CCTGGTGTACCAGGTGAAGG+TGG | 0.630185 | tig0030133:+2265 | Msa1442590:CDS |
| GCTCCAGATCCTATACCACC+AGG | 0.636137 | tig0030133:-2397 | None:intergenic |
| CCTAATGAAGAAGGATCTGG+AGG | 0.637880 | tig0030133:+2370 | Msa1442590:CDS |
| CTGCCTGGTGTACCAGGTGA+AGG | 0.641187 | tig0030133:+2262 | Msa1442590:CDS |
| AGTGGAATGTGCTCACCCGT+GGG | 0.649600 | tig0030133:-2346 | None:intergenic |
| ACACCACCAGGATATTCACC+AGG | 0.650451 | tig0030133:-2490 | None:intergenic |
| GGATCTGGAGCACTGCCTGG+AGG | 0.653536 | tig0030133:+2409 | Msa1442590:CDS |
| CCTGGTGGTGTGAATTCCGG+TGG | 0.656979 | tig0030133:+2502 | Msa1442590:CDS |
| GGTACTGGACCAGTTGAAGG+AGG | 0.657439 | tig0030133:+2586 | Msa1442590:CDS |
| TAGGAGTCATAGGATCTGGA+GGG | 0.670091 | tig0030133:+2227 | Msa1442590:CDS |
| GGAGACGGCTATCAACCCGT+TGG | 0.670699 | tig0030133:+2523 | Msa1442590:CDS |
| CTGCCTGGTGAATATCCTGG+TGG | 0.687649 | tig0030133:+2487 | Msa1442590:CDS |
| GGATCTGGAGGACTTCCTGG+TGG | 0.723484 | tig0030133:+2382 | Msa1442590:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GGAGTAAGTAGTTTAACAAT+AGG | + | tig0030133:2208-2227 | Msa1442590:CDS | 30.0% | |
| AGTTTAACAATAGGAGTCAT+AGG | + | tig0030133:2217-2236 | Msa1442590:CDS | 30.0% | |
| TCAGGTTTGATATATTAACC+AGG | - | tig0030133:2310-2329 | None:intergenic | 30.0% | |
| GGTTAATATATCAAACCTGA+TGG | + | tig0030133:2310-2329 | Msa1442590:intron | 30.0% | |
| TATATCAAACCTGATGGAAT+AGG | + | tig0030133:2316-2335 | Msa1442590:intron | 30.0% | |
| !! | TATACATAGCCTTTTTCATC+AGG | - | tig0030133:2652-2671 | None:intergenic | 30.0% |
| ATTCCACTTCCTAATGAAGA+AGG | + | tig0030133:2361-2380 | Msa1442590:CDS | 35.0% | |
| !! | TTGGGTGATGAAAAAGTACT+TGG | + | tig0030133:2460-2479 | Msa1442590:CDS | 35.0% |
| ACAATAGGAGTCATAGGATC+TGG | + | tig0030133:2223-2242 | Msa1442590:CDS | 40.0% | |
| GGAATATAGCCTATTCCATC+AGG | - | tig0030133:2328-2347 | None:intergenic | 40.0% | |
| TGGAATAGGCTATATTCCCA+CGG | + | tig0030133:2330-2349 | Msa1442590:intron | 40.0% | |
| GATCCTTCTTCATTAGGAAG+TGG | - | tig0030133:2367-2386 | None:intergenic | 40.0% | |
| CTTCCTAATGAAGAAGGATC+TGG | + | tig0030133:2367-2386 | Msa1442590:CDS | 40.0% | |
| TGTACCAGATGAAGGAGATT+TGG | + | tig0030133:2441-2460 | Msa1442590:CDS | 40.0% | |
| GTACCAGATGAAGGAGATTT+GGG | + | tig0030133:2442-2461 | Msa1442590:CDS | 40.0% | |
| !! | GGTGATGAAAAAGTACTTGG+AGG | + | tig0030133:2463-2482 | Msa1442590:CDS | 40.0% |
| ATAGGAGTCATAGGATCTGG+AGG | + | tig0030133:2226-2245 | Msa1442590:CDS | 45.0% | |
| TAGGAGTCATAGGATCTGGA+GGG | + | tig0030133:2227-2246 | Msa1442590:CDS | 45.0% | |
| GGAATAGGCTATATTCCCAC+GGG | + | tig0030133:2331-2350 | Msa1442590:intron | 45.0% | |
| CCTCCAGATCCTTCTTCATT+AGG | - | tig0030133:2373-2392 | None:intergenic | 45.0% | |
| CCTAATGAAGAAGGATCTGG+AGG | + | tig0030133:2370-2389 | Msa1442590:CDS | 45.0% | |
| TCACCCAAATCTCCTTCATC+TGG | - | tig0030133:2448-2467 | None:intergenic | 45.0% | |
| ! | TATCCTGGTGGTGTGAATTC+CGG | + | tig0030133:2499-2518 | Msa1442590:CDS | 45.0% |
| GGAGTTCTGCCTGATGAAAA+AGG | + | tig0030133:2640-2659 | Msa1442590:CDS | 45.0% | |
| TGTACCAGGTGAAGGTGGTT+TGG | + | tig0030133:2270-2289 | Msa1442590:CDS | 50.0% | |
| GTACCAGGTGAAGGTGGTTT+GGG | + | tig0030133:2271-2290 | Msa1442590:CDS | 50.0% | |
| CTTCCTGGTGGTATAGGATC+TGG | + | tig0030133:2394-2413 | Msa1442590:CDS | 50.0% | |
| CTTCGTGGTGTACCAGATGA+AGG | + | tig0030133:2433-2452 | Msa1442590:CDS | 50.0% | |
| ! | AAAGTACTTGGAGGACTGCC+TGG | + | tig0030133:2472-2491 | Msa1442590:CDS | 50.0% |
| ACACCACCAGGATATTCACC+AGG | - | tig0030133:2493-2512 | None:intergenic | 50.0% | |
| ACCAGGAACTTCTGCACCAA+CGG | - | tig0030133:2542-2561 | None:intergenic | 50.0% | |
| TCTTCCAGACCTCCTTCAAC+TGG | - | tig0030133:2598-2617 | None:intergenic | 50.0% | |
| GTTGAAGGAGGTCTGGAAGA+TGG | + | tig0030133:2598-2617 | Msa1442590:CDS | 50.0% | |
| GAACTCCTCTTTCATCAGGC+AGG | - | tig0030133:2627-2646 | None:intergenic | 50.0% | |
| GGCAGAACTCCTCTTTCATC+AGG | - | tig0030133:2631-2650 | None:intergenic | 50.0% | |
| GGATCTGGAGGGTTTCCTGT+AGG | + | tig0030133:2238-2257 | Msa1442590:CDS | 55.0% | |
| TCACCCAAACCACCTTCACC+TGG | - | tig0030133:2277-2296 | None:intergenic | 55.0% | |
| ! | GGTGAAGGTGGTTTGGGTGA+TGG | + | tig0030133:2277-2296 | Msa1442590:CDS | 55.0% |
| ! | GAAGGTGGTTTGGGTGATGG+AGG | + | tig0030133:2280-2299 | Msa1442590:CDS | 55.0% |
| !! | TTGGGTGATGGAGGACTTCC+TGG | + | tig0030133:2289-2308 | Msa1442590:CDS | 55.0% |
| AGTGGAATGTGCTCACCCGT+GGG | - | tig0030133:2349-2368 | None:intergenic | 55.0% | |
| AAGTGGAATGTGCTCACCCG+TGG | - | tig0030133:2350-2369 | None:intergenic | 55.0% | |
| GAAGGATCTGGAGGACTTCC+TGG | + | tig0030133:2379-2398 | Msa1442590:CDS | 55.0% | |
| GGAGGACTTCCTGGTGGTAT+AGG | + | tig0030133:2388-2407 | Msa1442590:CDS | 55.0% | |
| GCTCCAGATCCTATACCACC+AGG | - | tig0030133:2400-2419 | None:intergenic | 55.0% | |
| !! | ATAGGATCTGGAGCACTGCC+TGG | + | tig0030133:2406-2425 | Msa1442590:CDS | 55.0% |
| GGACTGCCTGGTGAATATCC+TGG | + | tig0030133:2484-2503 | Msa1442590:CDS | 55.0% | |
| CTGCCTGGTGAATATCCTGG+TGG | + | tig0030133:2487-2506 | Msa1442590:CDS | 55.0% | |
| GGTGTGAATTCCGGTGGAGA+CGG | + | tig0030133:2508-2527 | Msa1442590:CDS | 55.0% | |
| CCAGGAACTTCTGCACCAAC+GGG | - | tig0030133:2541-2560 | None:intergenic | 55.0% | |
| GGTGCAGAAGTTCCTGGTGA+AGG | + | tig0030133:2544-2563 | Msa1442590:CDS | 55.0% | |
| GCAGAAGTTCCTGGTGAAGG+AGG | + | tig0030133:2547-2566 | Msa1442590:CDS | 55.0% | |
| CCTTCATCTCCTCCTTCACC+AGG | - | tig0030133:2559-2578 | None:intergenic | 55.0% | |
| CCTGGTGAAGGAGGAGATGA+AGG | + | tig0030133:2556-2575 | Msa1442590:CDS | 55.0% | |
| GATGAAGGACTGCCTGGTAC+TGG | + | tig0030133:2571-2590 | Msa1442590:CDS | 55.0% | |
| CCTTCAACTGGTCCAGTACC+AGG | - | tig0030133:2586-2605 | None:intergenic | 55.0% | |
| ! | CCTGGTACTGGACCAGTTGA+AGG | + | tig0030133:2583-2602 | Msa1442590:CDS | 55.0% |
| ! | GGTACTGGACCAGTTGAAGG+AGG | + | tig0030133:2586-2605 | Msa1442590:CDS | 55.0% |
| TGGACCAGTTGAAGGAGGTC+TGG | + | tig0030133:2591-2610 | Msa1442590:CDS | 55.0% | |
| GGAGTCCTGCCTGATGAAAG+AGG | + | tig0030133:2619-2638 | Msa1442590:CDS | 55.0% | |
| GGGTTTCCTGTAGGACTGCC+TGG | + | tig0030133:2247-2266 | Msa1442590:CDS | 60.0% | |
| GGTACACCAGGCAGTCCTAC+AGG | - | tig0030133:2256-2275 | None:intergenic | 60.0% | |
| GTAGGACTGCCTGGTGTACC+AGG | + | tig0030133:2256-2275 | Msa1442590:CDS | 60.0% | |
| CTGCCTGGTGTACCAGGTGA+AGG | + | tig0030133:2262-2281 | Msa1442590:CDS | 60.0% | |
| CCACCTTCACCTGGTACACC+AGG | - | tig0030133:2268-2287 | None:intergenic | 60.0% | |
| CCTGGTGTACCAGGTGAAGG+TGG | + | tig0030133:2265-2284 | Msa1442590:CDS | 60.0% | |
| GGATCTGGAGGACTTCCTGG+TGG | + | tig0030133:2382-2401 | Msa1442590:CDS | 60.0% | |
| GGTACACCACGAAGTCCTCC+AGG | - | tig0030133:2427-2446 | None:intergenic | 60.0% | |
| CCACCGGAATTCACACCACC+AGG | - | tig0030133:2505-2524 | None:intergenic | 60.0% | |
| ! | CCTGGTGGTGTGAATTCCGG+TGG | + | tig0030133:2502-2521 | Msa1442590:CDS | 60.0% |
| !! | GGGTTGATAGCCGTCTCCAC+CGG | - | tig0030133:2521-2540 | None:intergenic | 60.0% |
| GGAGACGGCTATCAACCCGT+TGG | + | tig0030133:2523-2542 | Msa1442590:CDS | 60.0% | |
| CCCGTTGGTGCAGAAGTTCC+TGG | + | tig0030133:2538-2557 | Msa1442590:CDS | 60.0% | |
| GGAGGAGATGAAGGACTGCC+TGG | + | tig0030133:2565-2584 | Msa1442590:CDS | 60.0% | |
| !! | GGATCTGGAGCACTGCCTGG+AGG | + | tig0030133:2409-2428 | Msa1442590:CDS | 65.0% |
| GCACTGCCTGGAGGACTTCG+TGG | + | tig0030133:2418-2437 | Msa1442590:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0030133 | gene | 2199 | 2672 | 2199 | ID=Msa1442590;Name=Msa1442590 |
| tig0030133 | mRNA | 2199 | 2672 | 2199 | ID=Msa1442590-mRNA-1;Parent=Msa1442590;Name=Msa1442590-mRNA-1;_AED=0.10;_eAED=0.17;_QI=0|0|0|1|1|1|2|0|148 |
| tig0030133 | exon | 2199 | 2310 | 2199 | ID=Msa1442590-mRNA-1:exon:13394;Parent=Msa1442590-mRNA-1 |
| tig0030133 | exon | 2338 | 2672 | 2338 | ID=Msa1442590-mRNA-1:exon:13395;Parent=Msa1442590-mRNA-1 |
| tig0030133 | CDS | 2199 | 2310 | 2199 | ID=Msa1442590-mRNA-1:cds;Parent=Msa1442590-mRNA-1 |
| tig0030133 | CDS | 2338 | 2672 | 2338 | ID=Msa1442590-mRNA-1:cds;Parent=Msa1442590-mRNA-1 |
| Gene Sequence |
| Protein sequence |
