AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1217940


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1217940 MtrunA17_Chr4g0067541 92.982 114 7 1 1 113 1 114 1.44e-66 196
Msa1217940 MtrunA17_Chr2g0295651 60.684 117 33 3 1 109 1 112 4.06e-38 125
Msa1217940 MtrunA17_Chr3g0115551 46.970 66 31 1 38 103 71 132 4.95e-15 66.6
Msa1217940 MtrunA17_Chr8g0344991 40.541 74 36 1 36 101 6 79 5.09e-13 60.8
Msa1217940 MtrunA17_Chr4g0070221 52.083 48 22 1 56 103 173 219 7.13e-12 59.7
Msa1217940 MtrunA17_Chr3g0124361 38.889 72 41 1 35 103 95 166 2.76e-11 57.8
Msa1217940 MtrunA17_Chr3g0138651 53.191 47 22 0 56 102 349 395 7.18e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1217940 AT5G49120.1 62.264 53 20 0 56 108 63 115 4.25e-21 82.4
Msa1217940 AT1G22160.1 48.276 58 30 0 53 110 70 127 3.21e-14 64.7
Msa1217940 AT4G39795.1 42.029 69 39 1 33 101 46 113 4.21e-13 61.2
Msa1217940 AT1G78020.1 53.488 43 20 0 61 103 89 131 8.75e-13 61.2
Msa1217940 AT5G47060.2 56.000 50 21 1 61 110 97 145 7.38e-12 59.3
Msa1217940 AT5G47060.1 56.000 50 21 1 61 110 97 145 7.38e-12 59.3
Msa1217940 AT4G17670.1 52.000 50 23 1 61 110 76 124 9.18e-11 55.8

Find 19 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTAAACCATGGGTAAAAC+TGG 0.321231 8_1:+80483517 None:intergenic
AGAAAAGTTGAAACCTTAAA+AGG 0.340742 8_1:+80483983 None:intergenic
AACCTTAAAAGGAGAATGAT+AGG 0.405313 8_1:+80483994 None:intergenic
CACTGGAAATGTTGGGGTGG+GGG 0.412454 8_1:-80483574 Msa1217940:three_prime_UTR
TGCAGCAAGAAGCTCTTACC+TGG 0.449852 8_1:-80483944 Msa1217940:CDS
AGTAGTGACACTGGAAATGT+TGG 0.487421 8_1:-80483582 Msa1217940:exon
AAAGGAGAATGATAGGAAAG+TGG 0.494802 8_1:+80484001 None:intergenic
CCTACAATCCACGCTACAAA+AGG 0.495514 8_1:+80483663 None:intergenic
ACACTGGAAATGTTGGGGTG+GGG 0.537070 8_1:-80483575 Msa1217940:three_prime_UTR
ATCAGAGAATGAAATATGGT+TGG 0.543247 8_1:-80484160 Msa1217940:exon
GAATGAAATATGGTTGGTCT+TGG 0.548398 8_1:-80484154 Msa1217940:exon
GACACTGGAAATGTTGGGGT+GGG 0.558364 8_1:-80483576 Msa1217940:three_prime_UTR
TCTTGCTGCATAGAAAGCAT+TGG 0.559666 8_1:+80483957 None:intergenic
GTTGATTGTGCTAAGCATCA+TGG 0.561835 8_1:+80484039 None:intergenic
GTAGTGACACTGGAAATGTT+GGG 0.588242 8_1:-80483581 Msa1217940:exon
TACATGTAGATATCTTTCCC+AGG 0.593576 8_1:+80483926 None:intergenic
GCAGCAAGAAGCTCTTACCT+GGG 0.606797 8_1:-80483943 Msa1217940:CDS
TGACACTGGAAATGTTGGGG+TGG 0.614413 8_1:-80483577 Msa1217940:three_prime_UTR
TAGTGACACTGGAAATGTTG+GGG 0.702637 8_1:-80483580 Msa1217940:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTTTTTTTGCTA+TGG - chr8_1:80484173-80484192 Msa1217940:five_prime_UTR 10.0%
!! AAAAATCAGAGAATGAAATA+TGG - chr8_1:80483557-80483576 Msa1217940:three_prime_UTR 20.0%
!!! ATATTACTCTTTTTCTTATG+TGG + chr8_1:80483615-80483634 None:intergenic 20.0%
!!! CTTTTAATTTTATCTCCTAT+AGG - chr8_1:80483878-80483897 Msa1217940:intron 20.0%
!!! ATGCATTTTTTTTTTCTTCT+TGG - chr8_1:80483917-80483936 Msa1217940:intron 20.0%
!!! GAATTTTGTATTAGTATATG+TGG - chr8_1:80483986-80484005 Msa1217940:CDS 20.0%
!!! GAGTTATTTTTTTGTCTTAA+TGG - chr8_1:80484008-80484027 Msa1217940:CDS 20.0%
! AGAAAAGTTGAAACCTTAAA+AGG + chr8_1:80483741-80483760 None:intergenic 25.0%
! GTAAGATCTATATATCCTAT+AGG + chr8_1:80483896-80483915 None:intergenic 25.0%
! CAATTAACATCAAAAGATCT+GGG + chr8_1:80483967-80483986 None:intergenic 25.0%
! TCAATTAACATCAAAAGATC+TGG + chr8_1:80483968-80483987 None:intergenic 25.0%
! TCTGATTTTTCTTTCTGTTG+TGG + chr8_1:80483547-80483566 None:intergenic 30.0%
ATCAGAGAATGAAATATGGT+TGG - chr8_1:80483561-80483580 Msa1217940:three_prime_UTR 30.0%
! TTCCTATCATTCTCCTTTTA+AGG - chr8_1:80483725-80483744 Msa1217940:intron 30.0%
AACCTTAAAAGGAGAATGAT+AGG + chr8_1:80483730-80483749 None:intergenic 30.0%
! AATGCATGCTACACTTATTT+TGG + chr8_1:80483838-80483857 None:intergenic 30.0%
!!! ATCTGGGTATTTTTTCTGTA+AGG + chr8_1:80483951-80483970 None:intergenic 30.0%
!! GAATGAAATATGGTTGGTCT+TGG - chr8_1:80483567-80483586 Msa1217940:three_prime_UTR 35.0%
AAAGGAGAATGATAGGAAAG+TGG + chr8_1:80483723-80483742 None:intergenic 35.0%
TACATGTAGATATCTTTCCC+AGG + chr8_1:80483798-80483817 None:intergenic 35.0%
TACATGTACAAGTGAGTTCT+CGG - chr8_1:80483810-80483829 Msa1217940:intron 35.0%
!! TAGGTGCAAGCATATTTTAG+CGG - chr8_1:80484077-80484096 Msa1217940:CDS 35.0%
!! TTTTTGAAGAGTAGTGACAC+TGG - chr8_1:80484130-80484149 Msa1217940:CDS 35.0%
GTTGATTGTGCTAAGCATCA+TGG + chr8_1:80483685-80483704 None:intergenic 40.0%
! TCTTGCTGCATAGAAAGCAT+TGG + chr8_1:80483767-80483786 None:intergenic 40.0%
ATGGCACCTATTTATTGCAG+AGG - chr8_1:80484027-80484046 Msa1217940:CDS 40.0%
CTGTCTCCTCTGCAATAAAT+AGG + chr8_1:80484036-80484055 None:intergenic 40.0%
AGTAGTGACACTGGAAATGT+TGG - chr8_1:80484139-80484158 Msa1217940:CDS 40.0%
GTAGTGACACTGGAAATGTT+GGG - chr8_1:80484140-80484159 Msa1217940:CDS 40.0%
TAGTGACACTGGAAATGTTG+GGG - chr8_1:80484141-80484160 Msa1217940:CDS 40.0%
CCTACAATCCACGCTACAAA+AGG + chr8_1:80484061-80484080 None:intergenic 45.0%
!! CCTTTTGTAGCGTGGATTGT+AGG - chr8_1:80484058-80484077 Msa1217940:CDS 45.0%
!!! CAAGCATATTTTAGCGGACG+AGG - chr8_1:80484083-80484102 Msa1217940:CDS 45.0%
!!! TATTTTAGCGGACGAGGAAG+AGG - chr8_1:80484089-80484108 Msa1217940:CDS 45.0%
TGCAGCAAGAAGCTCTTACC+TGG - chr8_1:80483777-80483796 Msa1217940:intron 50.0%
GCAGCAAGAAGCTCTTACCT+GGG - chr8_1:80483778-80483797 Msa1217940:intron 50.0%
! AGACAGAGCCTTTTGTAGCG+TGG - chr8_1:80484050-80484069 Msa1217940:CDS 50.0%
TGACACTGGAAATGTTGGGG+TGG - chr8_1:80484144-80484163 Msa1217940:CDS 50.0%
GACACTGGAAATGTTGGGGT+GGG - chr8_1:80484145-80484164 Msa1217940:CDS 50.0%
ACACTGGAAATGTTGGGGTG+GGG - chr8_1:80484146-80484165 Msa1217940:CDS 50.0%
CACTGGAAATGTTGGGGTGG+GGG - chr8_1:80484147-80484166 Msa1217940:CDS 55.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 80483530 80484213 80483530 ID=Msa1217940;Name=Msa1217940
chr8_1 mRNA 80483530 80484213 80483530 ID=Msa1217940-mRNA-1;Parent=Msa1217940;Name=Msa1217940-mRNA-1;_AED=0.00;_eAED=0.00;_QI=46|1|1|1|1|1|2|70|113
chr8_1 exon 80483530 80483696 80483530 ID=Msa1217940-mRNA-1:exon:28195;Parent=Msa1217940-mRNA-1
chr8_1 exon 80483923 80484213 80483923 ID=Msa1217940-mRNA-1:exon:28194;Parent=Msa1217940-mRNA-1
chr8_1 five_prime_UTR 80484168 80484213 80484168 ID=Msa1217940-mRNA-1:five_prime_utr;Parent=Msa1217940-mRNA-1
chr8_1 CDS 80483923 80484167 80483923 ID=Msa1217940-mRNA-1:cds;Parent=Msa1217940-mRNA-1
chr8_1 CDS 80483600 80483696 80483600 ID=Msa1217940-mRNA-1:cds;Parent=Msa1217940-mRNA-1
chr8_1 three_prime_UTR 80483530 80483599 80483530 ID=Msa1217940-mRNA-1:three_prime_utr;Parent=Msa1217940-mRNA-1
Gene Sequence

>Msa1217940

ATGGTTGGTCTTGGTGTTGTGCTTGAAGAACAACAACCACATAAGAAAAAGAGTAATATCAGCATCAACAACAACAATAACACTTGTCAAGTTATTAACAAAACTACCATGATGCTTAGCACAATCAACAACAAAACTTCTTCTCCACTTTCCTATCATTCTCCTTTTAAGGTTTCAACTTTTCTTGACCAATGCTTTCTATGCAGCAAGAAGCTCTTACCTGGGAAAGATATCTACATGTACAAAGGAGACAGAGCCTTTTGTAGCGTGGATTGTAGGTGCAAGCATATTTTAGCGGACGAGGAAGAGGCTATGAAGAAACAGAACATTTTTGAAGAGTAG

Protein sequence

>Msa1217940

MVGLGVVLEEQQPHKKKSNISINNNNNTCQVINKTTMMLSTINNKTSSPLSYHSPFKVSTFLDQCFLCSKKLLPGKDIYMYKGDRAFCSVDCRCKHILADEEEAMKKQNIFEE*