AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1446030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1446030 MtrunA17_Chr8g0348991 95.592 363 16 0 16 378 1 363 0.0 713
Msa1446030 MtrunA17_Chr4g0063531 29.080 337 221 9 36 364 63 389 1.25e-33 129
Msa1446030 MtrunA17_Chr8g0378511 27.011 348 240 8 7 346 44 385 1.90e-31 123
Msa1446030 MtrunA17_Chr2g0285851 28.704 324 212 9 40 352 52 367 1.29e-29 118
Msa1446030 MtrunA17_Chr3g0122681 28.475 295 173 9 36 311 31 306 2.32e-29 116
Msa1446030 MtrunA17_Chr8g0377241 32.922 243 143 8 34 265 45 278 9.03e-29 115
Msa1446030 MtrunA17_Chr3g0129221 29.924 264 161 8 35 291 4 250 2.16e-26 108
Msa1446030 MtrunA17_Chr7g0260051 26.923 312 192 11 40 336 4 294 1.74e-19 88.6
Msa1446030 MtrunA17_Chr2g0278921 28.571 238 153 6 36 267 39 265 3.18e-18 85.9
Msa1446030 MtrunA17_Chr3g0115401 23.276 348 195 13 36 314 39 383 6.70e-18 85.1
Msa1446030 MtrunA17_Chr4g0060541 23.913 322 214 9 17 319 7 316 5.16e-16 79.0
Msa1446030 MtrunA17_Chr3g0138981 23.704 270 176 9 21 285 5 249 7.10e-15 75.1
Msa1446030 MtrunA17_Chr2g0326541 23.239 284 202 6 37 314 17 290 3.86e-12 67.0
Msa1446030 MtrunA17_Chr4g0011151 24.051 237 160 6 13 245 78 298 4.60e-12 67.0
Msa1446030 MtrunA17_Chr4g0011221 24.051 237 160 6 13 245 78 298 1.24e-11 65.9
Msa1446030 MtrunA17_Chr4g0011161 23.629 237 161 6 13 245 78 298 1.29e-11 65.9
Msa1446030 MtrunA17_Chr4g0011191 24.051 237 160 6 13 245 78 298 1.77e-11 64.7
Msa1446030 MtrunA17_Chr4g0011171 23.629 237 161 6 13 245 78 298 2.49e-11 64.7
Msa1446030 MtrunA17_Chr4g0010161 24.675 231 161 5 40 267 68 288 2.79e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1446030 AT3G61350.2 53.936 343 149 5 40 378 21 358 4.69e-124 362
Msa1446030 AT3G61350.1 53.936 343 149 5 40 378 21 358 4.69e-124 362
Msa1446030 AT1G55270.1 28.205 351 236 8 7 348 45 388 5.71e-33 128
Msa1446030 AT2G24540.1 24.796 367 252 9 27 378 15 372 9.28e-29 115
Msa1446030 AT1G67480.3 29.739 306 185 11 18 311 23 310 1.76e-25 106
Msa1446030 AT1G67480.1 29.739 306 185 11 18 311 23 310 1.76e-25 106
Msa1446030 AT1G67480.2 29.739 306 185 11 18 311 23 310 1.76e-25 106
Msa1446030 AT1G30090.1 26.506 332 219 12 31 346 43 365 4.35e-25 105
Msa1446030 AT4G39590.1 24.650 357 224 8 13 335 1 346 1.92e-20 92.4
Msa1446030 AT1G27420.1 24.739 287 191 8 35 308 5 279 2.10e-20 91.7
Msa1446030 AT1G55270.2 27.509 269 179 8 89 348 1 262 3.42e-19 87.8
Msa1446030 AT1G22040.1 25.562 356 188 12 24 311 32 378 1.15e-18 87.8
Msa1446030 AT3G63220.3 24.367 316 195 11 40 336 11 301 9.73e-18 84.0
Msa1446030 AT3G63220.2 24.367 316 195 11 40 336 11 301 9.73e-18 84.0
Msa1446030 AT2G29830.1 23.599 339 221 13 22 338 9 331 1.08e-17 84.3
Msa1446030 AT3G63220.1 24.367 316 195 11 40 336 4 294 1.15e-17 83.6
Msa1446030 AT2G21950.1 26.568 271 176 10 33 291 13 272 7.13e-17 81.6
Msa1446030 AT1G16250.2 29.381 194 115 7 40 218 8 194 2.59e-16 80.1
Msa1446030 AT1G16250.1 29.381 194 115 7 40 218 8 194 2.59e-16 80.1
Msa1446030 AT4G39240.1 29.804 255 149 10 44 285 37 274 5.56e-14 73.2
Msa1446030 AT5G60570.2 22.000 300 216 8 26 318 35 323 1.02e-13 72.4
Msa1446030 AT5G60570.1 22.000 300 216 8 26 318 35 323 1.02e-13 72.4
Msa1446030 AT5G60570.3 22.000 300 216 8 26 318 35 323 1.02e-13 72.4
Msa1446030 AT3G43710.1 26.556 241 156 9 44 275 29 257 1.45e-13 72.0
Msa1446030 AT4G39550.1 25.095 263 153 7 44 276 27 275 4.15e-13 70.5
Msa1446030 AT1G14330.1 23.077 312 207 11 23 314 72 370 4.82e-13 70.5
Msa1446030 AT2G02870.2 23.151 311 207 10 23 314 100 397 5.58e-13 70.5
Msa1446030 AT2G02870.3 23.151 311 207 10 23 314 100 397 5.58e-13 70.5
Msa1446030 AT2G02870.1 23.151 311 207 10 23 314 100 397 5.58e-13 70.5
Msa1446030 AT4G39290.1 30.159 189 121 6 44 225 16 200 2.49e-12 68.2
Msa1446030 AT4G39756.1 23.765 324 202 10 30 330 9 310 3.26e-12 67.8
Msa1446030 AT4G38940.1 25.338 296 190 12 40 318 20 301 6.00e-12 67.0
Msa1446030 AT1G61540.1 24.107 336 211 10 34 335 20 345 6.80e-12 67.0
Msa1446030 AT5G49000.2 27.668 253 136 10 44 272 25 254 2.20e-11 65.1
Msa1446030 AT4G23580.1 26.154 260 147 9 44 276 19 260 2.31e-11 65.1
Msa1446030 AT4G39580.1 25.484 310 197 12 44 335 26 319 5.21e-11 63.9
Msa1446030 AT4G34170.1 24.299 321 197 10 19 335 1 279 9.19e-11 62.8

Find 102 sgRNAs with CRISPR-Local

Find 203 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGAAATAAGCTCTTCTTAT+TGG 0.156806 tig0031256:-44330 Msa1446030:CDS
TCAATTTCGGGAACTATATT+AGG 0.164167 tig0031256:+42733 None:intergenic
GGTAAAAGCATTTATATTAT+TGG 0.186633 tig0031256:-42418 Msa1446030:CDS
TGCTTTGTGCAGGGATAAAT+TGG 0.190481 tig0031256:-44465 Msa1446030:CDS
ACTATTGAATCTTCAATTTC+GGG 0.198008 tig0031256:+42721 None:intergenic
GCAGACACCGACATGGTTTC+AGG 0.213853 tig0031256:-42601 Msa1446030:CDS
AACTATTGAATCTTCAATTT+CGG 0.215275 tig0031256:+42720 None:intergenic
TGTTGGTGATATTGGAAATA+TGG 0.261504 tig0031256:-42368 Msa1446030:CDS
GGAAATAAGCTCTTCTTATT+GGG 0.298222 tig0031256:-44329 Msa1446030:CDS
TCATGTTTACTGTTACGTTT+TGG 0.309221 tig0031256:-44441 Msa1446030:CDS
GTTGTTGATGTTGGTGATAT+TGG 0.315849 tig0031256:-42376 Msa1446030:CDS
ACCAACTGCAACAAGTTTGC+AGG 0.334342 tig0031256:+42438 None:intergenic
AGCATTTATATTATTGGAAA+AGG 0.341860 tig0031256:-42412 Msa1446030:CDS
ATTCAAGTGATCCTCATTCT+TGG 0.348607 tig0031256:-42801 Msa1446030:CDS
TACGTGTTAGAATTTCAGTT+TGG 0.357385 tig0031256:-44845 Msa1446030:five_prime_UTR
TGTTGCCATGTGCCATTTGA+TGG 0.360250 tig0031256:+42625 None:intergenic
GCATACACATGAGGTGTTAA+AGG 0.366134 tig0031256:+42661 None:intergenic
AATTGAAGATTCAATAGTTA+TGG 0.374204 tig0031256:-42716 Msa1446030:CDS
AATGAATTGAAATGATGATT+TGG 0.376983 tig0031256:-44783 Msa1446030:five_prime_UTR
TTGTGATCAGAGACTAATTT+CGG 0.379853 tig0031256:+42319 None:intergenic
GTCACAATTGGTGACAGGTT+TGG 0.396360 tig0031256:+42128 None:intergenic
CTCGAAAAGATGGAGGGACT+TGG 0.398078 tig0031256:-44549 Msa1446030:CDS
TCTTATTGGGTGGCTGTGGC+TGG 0.404326 tig0031256:-44316 Msa1446030:CDS
AACCCCATGAATGAGCTTCC+AGG 0.418653 tig0031256:+44397 None:intergenic
ATTTCAGTTTGGGAACATAA+TGG 0.418838 tig0031256:-44834 Msa1446030:five_prime_UTR
AAATATGGGAAGGGCGATAC+CGG 0.429102 tig0031256:-42353 Msa1446030:CDS
ACGTGTTAGAATTTCAGTTT+GGG 0.430731 tig0031256:-44844 Msa1446030:five_prime_UTR
GAACTCTGATCCAAAACAAA+AGG 0.430781 tig0031256:+42544 None:intergenic
TTCAAGTGATCCTCATTCTT+GGG 0.430876 tig0031256:-42800 Msa1446030:CDS
AAATCCTGGAAGCTCATTCA+TGG 0.431377 tig0031256:-44401 Msa1446030:CDS
CTCTTCTTATTGGGTGGCTG+TGG 0.432373 tig0031256:-44320 Msa1446030:CDS
AATAATACTCCACTGATTTG+TGG 0.437163 tig0031256:-44644 Msa1446030:CDS
AGAGAGACGCGAGTGGATAC+TGG 0.442798 tig0031256:-42494 Msa1446030:CDS
CACAAAAGAGAAATATCATC+TGG 0.445413 tig0031256:+44617 None:intergenic
GCATGGGACATTGTCACAAT+TGG 0.459141 tig0031256:+42116 None:intergenic
AGCACTGTGGTTGTTGATGT+TGG 0.464649 tig0031256:-42385 Msa1446030:CDS
TGAAGGCTGTCTCGAAAAGA+TGG 0.470081 tig0031256:-44559 Msa1446030:CDS
TGGATCAGAGTTCGGGTACT+AGG 0.474624 tig0031256:-42534 Msa1446030:CDS
GTTGGTGATATTGGAAATAT+GGG 0.477547 tig0031256:-42367 Msa1446030:CDS
CTATATGCTATTGGTGGCAT+AGG 0.479990 tig0031256:-42829 Msa1446030:CDS
GCATTTATATTATTGGAAAA+GGG 0.482758 tig0031256:-42411 Msa1446030:CDS
GTGAGATGTCCAACCATTTG+TGG 0.487502 tig0031256:+42762 None:intergenic
TTCCACCTCACGTCATGAAA+AGG 0.490506 tig0031256:-44376 Msa1446030:CDS
CATGGATCTATGCTTTGTGC+AGG 0.492337 tig0031256:-44475 Msa1446030:CDS
ACAATGCTTCTTTGAACTCG+TGG 0.495071 tig0031256:-44262 Msa1446030:CDS
AGTTCCTAGAAAATATCATT+CGG 0.505984 tig0031256:-44585 Msa1446030:CDS
GTTGTATACGATCCATCAAA+TGG 0.507083 tig0031256:-42637 Msa1446030:CDS
CAGACACCGACATGGTTTCA+GGG 0.513817 tig0031256:-42600 Msa1446030:CDS
CGTCATGAAAAGGAAAGGAA+TGG 0.514213 tig0031256:-44366 Msa1446030:CDS
ATGTCATTTGTGAACAATGA+AGG 0.521789 tig0031256:+42012 None:intergenic
CTACCTCGTCAAGAAAATCC+TGG 0.524735 tig0031256:-44415 Msa1446030:CDS
CCCTGCAAACTTGTTGCAGT+TGG 0.525074 tig0031256:-42439 Msa1446030:CDS
ACATTGTCACAATTGGTGAC+AGG 0.528710 tig0031256:+42123 None:intergenic
CAATGCTTCTTTGAACTCGT+GGG 0.529590 tig0031256:-44261 Msa1446030:CDS
TTGAAATGATGATTTGGTGT+TGG 0.531365 tig0031256:-44777 Msa1446030:five_prime_UTR
CCGTCGTCTAAATGCTCCAT+TGG 0.532539 tig0031256:+44707 None:intergenic
TTGATCCTTCCACAAATGGT+TGG 0.534149 tig0031256:-42771 Msa1446030:CDS
AATCCTGGAAGCTCATTCAT+GGG 0.540754 tig0031256:-44400 Msa1446030:CDS
GTCTTCTGAATTAATACCAA+TGG 0.542171 tig0031256:-44723 Msa1446030:CDS
GTCATGAAAAGGAAAGGAAT+GGG 0.545551 tig0031256:-44365 Msa1446030:CDS
CTACTTTGCTTGTGAAGCAA+TGG 0.547084 tig0031256:-42860 Msa1446030:CDS
AACTCTGATCCAAAACAAAA+GGG 0.547129 tig0031256:+42545 None:intergenic
CCAACTGCAACAAGTTTGCA+GGG 0.547956 tig0031256:+42439 None:intergenic
AATATGGGAAGGGCGATACC+GGG 0.551495 tig0031256:-42352 Msa1446030:CDS
CTGCTTCCTTGTCAACTGCT+AGG 0.568618 tig0031256:-44232 Msa1446030:intron
AATAAGCTCTTCTTATTGGG+TGG 0.572942 tig0031256:-44326 Msa1446030:CDS
GTGATATTGGAAATATGGGA+AGG 0.573342 tig0031256:-42363 Msa1446030:CDS
AAAGAGAAATATCATCTGGA+AGG 0.578717 tig0031256:+44621 None:intergenic
ACTTGGTTTGCAGCGAAGAG+TGG 0.580873 tig0031256:-44532 Msa1446030:CDS
AGCATAAACTTGATGAGACA+TGG 0.581062 tig0031256:-44493 Msa1446030:CDS
GAAGATTCAATAGTTATGGA+CGG 0.582095 tig0031256:-42712 Msa1446030:CDS
TCAAAAGTATCCCAAGAATG+AGG 0.583466 tig0031256:+42790 None:intergenic
ATTCCGTTGACAACAACTGC+AGG 0.584997 tig0031256:+42568 None:intergenic
GGCTGTCTCGAAAAGATGGA+GGG 0.589666 tig0031256:-44555 Msa1446030:CDS
CAGGGTGGTCTTGTAAGCAA+CGG 0.599662 tig0031256:+42457 None:intergenic
GGTCCTGCAGTTGTTGTCAA+CGG 0.619586 tig0031256:-42571 Msa1446030:CDS
TCTGGAAGGCCACAAATCAG+TGG 0.621422 tig0031256:+44635 None:intergenic
CCTCACGTCATGAAAAGGAA+AGG 0.622753 tig0031256:-44371 Msa1446030:CDS
CGGGTACTAGGTTGACGATG+TGG 0.627913 tig0031256:-42522 Msa1446030:CDS
TGATATTGGAAATATGGGAA+GGG 0.630143 tig0031256:-42362 Msa1446030:CDS
GACATGGTTTCAGGGTGGAG+AGG 0.635125 tig0031256:-42592 Msa1446030:CDS
GATGTCCAACCATTTGTGGA+AGG 0.636239 tig0031256:+42766 None:intergenic
AGGCTGTCTCGAAAAGATGG+AGG 0.640959 tig0031256:-44556 Msa1446030:CDS
TATTTACCTAGCAGTTGACA+AGG 0.645117 tig0031256:+44226 None:intergenic
ATGGATCTATGCTTTGTGCA+GGG 0.650304 tig0031256:-44474 Msa1446030:CDS
TGGAAAAGGGCTTAGCACTG+TGG 0.661995 tig0031256:-42398 Msa1446030:CDS
GCAACATGCAGACACCGACA+TGG 0.663606 tig0031256:-42608 Msa1446030:CDS
TATACAACGGCATACACATG+AGG 0.672079 tig0031256:+42652 None:intergenic
ATCCTGGAAGCTCATTCATG+GGG 0.677577 tig0031256:-44399 Msa1446030:CDS
TCTCCACCCTGAAACCATGT+CGG 0.677757 tig0031256:+42594 None:intergenic
GGCATAAAGAGAGACGCGAG+TGG 0.682691 tig0031256:-42501 Msa1446030:CDS
ACGATCCATCAAATGGCACA+TGG 0.684559 tig0031256:-42630 Msa1446030:CDS
TGTTGAGGCACATAGATGTG+TGG 0.692330 tig0031256:-42042 Msa1446030:three_prime_UTR
CCAATGGAGCATTTAGACGA+CGG 0.695363 tig0031256:-44707 Msa1446030:CDS
TCATTTGTGAACAATGAAGG+TGG 0.700318 tig0031256:+42015 None:intergenic
AGACGCGAGTGGATACTGGT+CGG 0.708143 tig0031256:-42490 Msa1446030:CDS
GGCAAAATATACATCCGATG+TGG 0.708393 tig0031256:-42691 Msa1446030:CDS
ATTTGATGGATCGTATACAA+CGG 0.723109 tig0031256:+42639 None:intergenic
TTAAAGGAGATTTACCACAT+CGG 0.724075 tig0031256:+42677 None:intergenic
AATTTCGGTATAGAAGAACC+CGG 0.724097 tig0031256:+42334 None:intergenic
ACACCGACATGGTTTCAGGG+TGG 0.734093 tig0031256:-42597 Msa1446030:CDS
ACTGCAACAAGTTTGCAGGG+TGG 0.781995 tig0031256:+42442 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGAAATATTGTAAAAGAAAA+TGG + tig0031256:42730-42749 None:intergenic 15.0%
!! TTTACTTGGAAATAAATATT+TGG - tig0031256:42954-42973 Msa1446030:intron 15.0%
!! TGAATGAAAATTGAAAATAA+GGG - tig0031256:42994-43013 Msa1446030:intron 15.0%
!!! TTTGTATGATGATTTTATTA+AGG - tig0031256:44663-44682 Msa1446030:CDS 15.0%
!! AATGAATTGAAATGATGATT+TGG - tig0031256:42065-42084 Msa1446030:three_prime_UTR 20.0%
!!! CATGTGTATTTTTATTTTTC+AGG - tig0031256:42103-42122 Msa1446030:three_prime_UTR 20.0%
!!! AAAACAGATCTAACATTTTT+CGG - tig0031256:42749-42768 Msa1446030:CDS 20.0%
!!! GATTTTCATAAACAATGTTT+TGG + tig0031256:42789-42808 None:intergenic 20.0%
!!! AAATGTTATTTGAGTAAACA+TGG - tig0031256:42922-42941 Msa1446030:intron 20.0%
!! GTGAATGAAAATTGAAAATA+AGG - tig0031256:42993-43012 Msa1446030:intron 20.0%
!! ATAAATATGAAAGGAACAAA+TGG + tig0031256:43107-43126 None:intergenic 20.0%
!!! ATTTTCCTTCTCTTTTTTTT+GGG - tig0031256:43323-43342 Msa1446030:intron 20.0%
!! ATATTTAACTAGCACAAAAA+AGG + tig0031256:43378-43397 None:intergenic 20.0%
!!! GTTTTATATTACTACTATTG+AGG - tig0031256:43530-43549 Msa1446030:intron 20.0%
!! TTAACTATTTACTATCTGTT+TGG - tig0031256:43649-43668 Msa1446030:intron 20.0%
!! TAACTATTTACTATCTGTTT+GGG - tig0031256:43650-43669 Msa1446030:intron 20.0%
!! ATGATTCTTATATTACAGTA+GGG + tig0031256:43940-43959 None:intergenic 20.0%
!! AACTATTGAATCTTCAATTT+CGG + tig0031256:44131-44150 None:intergenic 20.0%
!! AATTGAAGATTCAATAGTTA+TGG - tig0031256:44132-44151 Msa1446030:intron 20.0%
!!! GGTAAAAGCATTTATATTAT+TGG - tig0031256:44430-44449 Msa1446030:CDS 20.0%
!! AGCATTTATATTATTGGAAA+AGG - tig0031256:44436-44455 Msa1446030:CDS 20.0%
!! GCATTTATATTATTGGAAAA+GGG - tig0031256:44437-44456 Msa1446030:CDS 20.0%
!! AAAAAGTAGAAAATTTGCAT+GGG + tig0031256:44751-44770 None:intergenic 20.0%
!! TAAAAAGTAGAAAATTTGCA+TGG + tig0031256:44752-44771 None:intergenic 20.0%
! AGTTCCTAGAAAATATCATT+CGG - tig0031256:42263-42282 Msa1446030:three_prime_UTR 25.0%
!! AAAACCGAATGATATTTTCT+AGG + tig0031256:42270-42289 None:intergenic 25.0%
!!! AAAATATCATTCGGTTTTGA+AGG - tig0031256:42272-42291 Msa1446030:exon 25.0%
! TATTGTAAAAGAAAATGGTG+AGG + tig0031256:42725-42744 None:intergenic 25.0%
!! GATTTTCCTTCTCTTTTTTT+TGG - tig0031256:43322-43341 Msa1446030:intron 25.0%
!!! TTTTCCTTCTCTTTTTTTTG+GGG - tig0031256:43324-43343 Msa1446030:intron 25.0%
! TTGGCAAAATATCATTAATG+AGG - tig0031256:43466-43485 Msa1446030:intron 25.0%
! ATTACTACTATTGAGGAATA+AGG - tig0031256:43537-43556 Msa1446030:intron 25.0%
! AAGATGAATTAGAAAAAGAC+AGG + tig0031256:43587-43606 None:intergenic 25.0%
!!! ATGCAATTTTAGCTTTGAAT+AGG + tig0031256:43895-43914 None:intergenic 25.0%
! TGATTCTTATATTACAGTAG+GGG + tig0031256:43939-43958 None:intergenic 25.0%
! CATGATTCTTATATTACAGT+AGG + tig0031256:43941-43960 None:intergenic 25.0%
! ACTATTGAATCTTCAATTTC+GGG + tig0031256:44130-44149 None:intergenic 25.0%
TACGTGTTAGAATTTCAGTT+TGG - tig0031256:42003-42022 Msa1446030:three_prime_UTR 30.0%
ACGTGTTAGAATTTCAGTTT+GGG - tig0031256:42004-42023 Msa1446030:three_prime_UTR 30.0%
ATTTCAGTTTGGGAACATAA+TGG - tig0031256:42014-42033 Msa1446030:three_prime_UTR 30.0%
!! TTGAAATGATGATTTGGTGT+TGG - tig0031256:42071-42090 Msa1446030:three_prime_UTR 30.0%
GTCTTCTGAATTAATACCAA+TGG - tig0031256:42125-42144 Msa1446030:three_prime_UTR 30.0%
!! AATAATACTCCACTGATTTG+TGG - tig0031256:42204-42223 Msa1446030:three_prime_UTR 30.0%
AAAGAGAAATATCATCTGGA+AGG + tig0031256:42230-42249 None:intergenic 30.0%
CACAAAAGAGAAATATCATC+TGG + tig0031256:42234-42253 None:intergenic 30.0%
! TGATATTTCTCTTTTGTGTC+TGG - tig0031256:42236-42255 Msa1446030:three_prime_UTR 30.0%
! TCATGTTTACTGTTACGTTT+TGG - tig0031256:42407-42426 Msa1446030:CDS 30.0%
GGAAATAAGCTCTTCTTATT+GGG - tig0031256:42519-42538 Msa1446030:CDS 30.0%
TGATTAACTTAGCTTCTGAA+GGG + tig0031256:42661-42680 None:intergenic 30.0%
GAAAATGGTGAGGAAAATAT+AGG + tig0031256:42715-42734 None:intergenic 30.0%
GAAAATCTATTCTTCCTAAC+TGG - tig0031256:42802-42821 Msa1446030:CDS 30.0%
AATTGTGATAGAGAGAACAT+GGG + tig0031256:42831-42850 None:intergenic 30.0%
TAATTGTGATAGAGAGAACA+TGG + tig0031256:42832-42851 None:intergenic 30.0%
!! CATGGTCTTAGTATTTTACT+TGG - tig0031256:42940-42959 Msa1446030:intron 30.0%
TTCATTCACAGTAAAACTTG+TGG + tig0031256:42982-43001 None:intergenic 30.0%
AAAATAAGGGTAGTGAGAAT+AGG - tig0031256:43007-43026 Msa1446030:intron 30.0%
AAATAAGGGTAGTGAGAATA+GGG - tig0031256:43008-43027 Msa1446030:intron 30.0%
AATATGAAAGGAACAAATGG+AGG + tig0031256:43104-43123 None:intergenic 30.0%
ATTTGATCTTTCACATGCTT+AGG - tig0031256:43241-43260 Msa1446030:intron 30.0%
GTTCCTTAACTTCAAAAATG+TGG - tig0031256:43284-43303 Msa1446030:intron 30.0%
!!! GTACCACATTTTTGAAGTTA+AGG + tig0031256:43290-43309 None:intergenic 30.0%
ACATGCTTGTATATTTGACT+TGG - tig0031256:43447-43466 Msa1446030:intron 30.0%
TATTTACTATCTGTTTGGGT+TGG - tig0031256:43654-43673 Msa1446030:intron 30.0%
!! GTATTGATTAAGTGTGTGTT+TGG + tig0031256:43799-43818 None:intergenic 30.0%
CACTTAATCAATACCTACTT+TGG - tig0031256:43805-43824 Msa1446030:intron 30.0%
!!! TCCTTCACATTTTTTTTTCC+AGG - tig0031256:43963-43982 Msa1446030:intron 30.0%
ACCTGGAAAAAAAAATGTGA+AGG + tig0031256:43967-43986 None:intergenic 30.0%
GACGAAAAACTATATGCTAT+TGG - tig0031256:44010-44029 Msa1446030:intron 30.0%
GAAAAACTATATGCTATTGG+TGG - tig0031256:44013-44032 Msa1446030:intron 30.0%
TCAATTTCGGGAACTATATT+AGG + tig0031256:44118-44137 None:intergenic 30.0%
GAAGATTCAATAGTTATGGA+CGG - tig0031256:44136-44155 Msa1446030:intron 30.0%
TTAAAGGAGATTTACCACAT+CGG + tig0031256:44174-44193 None:intergenic 30.0%
! ATTTGATGGATCGTATACAA+CGG + tig0031256:44212-44231 None:intergenic 30.0%
AACTCTGATCCAAAACAAAA+GGG + tig0031256:44306-44325 None:intergenic 30.0%
!!! TTTTGTTTTGGATCAGAGTT+CGG - tig0031256:44306-44325 Msa1446030:CDS 30.0%
!! TGTTGGTGATATTGGAAATA+TGG - tig0031256:44480-44499 Msa1446030:CDS 30.0%
!! GTTGGTGATATTGGAAATAT+GGG - tig0031256:44481-44500 Msa1446030:CDS 30.0%
TGATATTGGAAATATGGGAA+GGG - tig0031256:44486-44505 Msa1446030:CDS 30.0%
TTGTGATCAGAGACTAATTT+CGG + tig0031256:44532-44551 None:intergenic 30.0%
!! TGAGCAATTGATTTTTGTTG+AGG - tig0031256:44791-44810 Msa1446030:five_prime_UTR 30.0%
! ATGTCATTTGTGAACAATGA+AGG + tig0031256:44839-44858 None:intergenic 30.0%
AGCATAAACTTGATGAGACA+TGG - tig0031256:42355-42374 Msa1446030:CDS 35.0%
GTCATGAAAAGGAAAGGAAT+GGG - tig0031256:42483-42502 Msa1446030:CDS 35.0%
GGGAAATAAGCTCTTCTTAT+TGG - tig0031256:42518-42537 Msa1446030:CDS 35.0%
AATAAGCTCTTCTTATTGGG+TGG - tig0031256:42522-42541 Msa1446030:CDS 35.0%
TATTTACCTAGCAGTTGACA+AGG + tig0031256:42625-42644 None:intergenic 35.0%
GTGATTAACTTAGCTTCTGA+AGG + tig0031256:42662-42681 None:intergenic 35.0%
AACTCCCCAAAAAAAAGAGA+AGG + tig0031256:43331-43350 None:intergenic 35.0%
!!! TTCTCTTTTTTTTGGGGAGT+TGG - tig0031256:43330-43349 Msa1446030:intron 35.0%
! GAAAAAGACAGGTGTGTTTT+GGG + tig0031256:43576-43595 None:intergenic 35.0%
! AGAAAAAGACAGGTGTGTTT+TGG + tig0031256:43577-43596 None:intergenic 35.0%
TACTTTCGTGGAAATCTAGA+AGG - tig0031256:43710-43729 Msa1446030:intron 35.0%
GCTTAATTCTTTCAAAACGC+CGG + tig0031256:43772-43791 None:intergenic 35.0%
GATTAAGTGTGTGTTTGGAA+TGG + tig0031256:43794-43813 None:intergenic 35.0%
ATTCAAGTGATCCTCATTCT+TGG - tig0031256:44047-44066 Msa1446030:intron 35.0%
TTCAAGTGATCCTCATTCTT+GGG - tig0031256:44048-44067 Msa1446030:intron 35.0%
!! TCAAAAGTATCCCAAGAATG+AGG + tig0031256:44061-44080 None:intergenic 35.0%
! ACTTTTGATCCTTCCACAAA+TGG - tig0031256:44073-44092 Msa1446030:intron 35.0%
GTTGTATACGATCCATCAAA+TGG - tig0031256:44211-44230 Msa1446030:intron 35.0%
GAACTCTGATCCAAAACAAA+AGG + tig0031256:44307-44326 None:intergenic 35.0%
!!! TTTGTTTTGGATCAGAGTTC+GGG - tig0031256:44307-44326 Msa1446030:CDS 35.0%
!! GTTGTTGATGTTGGTGATAT+TGG - tig0031256:44472-44491 Msa1446030:CDS 35.0%
GTGATATTGGAAATATGGGA+AGG - tig0031256:44485-44504 Msa1446030:CDS 35.0%
AATTTCGGTATAGAAGAACC+CGG + tig0031256:44517-44536 None:intergenic 35.0%
TCATTTGTGAACAATGAAGG+TGG + tig0031256:44836-44855 None:intergenic 35.0%
ATGGATCTATGCTTTGTGCA+GGG - tig0031256:42374-42393 Msa1446030:CDS 40.0%
TGCTTTGTGCAGGGATAAAT+TGG - tig0031256:42383-42402 Msa1446030:CDS 40.0%
AAATCCTGGAAGCTCATTCA+TGG - tig0031256:42447-42466 Msa1446030:CDS 40.0%
AATCCTGGAAGCTCATTCAT+GGG - tig0031256:42448-42467 Msa1446030:CDS 40.0%
CGTCATGAAAAGGAAAGGAA+TGG - tig0031256:42482-42501 Msa1446030:CDS 40.0%
!!! AGGAATGGGTTTTGAAGCTT+TGG - tig0031256:42497-42516 Msa1446030:CDS 40.0%
!!! GGAATGGGTTTTGAAGCTTT+GGG - tig0031256:42498-42517 Msa1446030:CDS 40.0%
ACAATGCTTCTTTGAACTCG+TGG - tig0031256:42586-42605 Msa1446030:CDS 40.0%
CAATGCTTCTTTGAACTCGT+GGG - tig0031256:42587-42606 Msa1446030:CDS 40.0%
CCTATGTAGAATGCAACTGT+AGG + tig0031256:43192-43211 None:intergenic 40.0%
CCTACAGTTGCATTCTACAT+AGG - tig0031256:43189-43208 Msa1446030:intron 40.0%
ACAGTTGCATTCTACATAGG+CGG - tig0031256:43192-43211 Msa1446030:intron 40.0%
!!! CATCTTTTTTGAAGCCTGCT+AGG - tig0031256:43601-43620 Msa1446030:intron 40.0%
AAAACTTCCACTTTCCTAGC+AGG + tig0031256:43618-43637 None:intergenic 40.0%
!! TTACTATCTGTTTGGGTTGG+CGG - tig0031256:43657-43676 Msa1446030:intron 40.0%
AAAGTAGCTCCTTGAAACGT+GGG + tig0031256:43696-43715 None:intergenic 40.0%
AATTCTTTCAAAACGCCGGT+AGG + tig0031256:43768-43787 None:intergenic 40.0%
! CATGATAACAAGAGAGCATG+GGG + tig0031256:43831-43850 None:intergenic 40.0%
! CCATGATAACAAGAGAGCAT+GGG + tig0031256:43832-43851 None:intergenic 40.0%
ACCATGATAACAAGAGAGCA+TGG + tig0031256:43833-43852 None:intergenic 40.0%
CTACTTTGCTTGTGAAGCAA+TGG - tig0031256:43988-44007 Msa1446030:intron 40.0%
! CTATATGCTATTGGTGGCAT+AGG - tig0031256:44019-44038 Msa1446030:intron 40.0%
TTGATCCTTCCACAAATGGT+TGG - tig0031256:44077-44096 Msa1446030:intron 40.0%
GGCAAAATATACATCCGATG+TGG - tig0031256:44157-44176 Msa1446030:intron 40.0%
! GCATACACATGAGGTGTTAA+AGG + tig0031256:44190-44209 None:intergenic 40.0%
TATACAACGGCATACACATG+AGG + tig0031256:44199-44218 None:intergenic 40.0%
!! CAACGGAATCCCTTTTGTTT+TGG - tig0031256:44294-44313 Msa1446030:CDS 40.0%
! ACATTGTCACAATTGGTGAC+AGG + tig0031256:44728-44747 None:intergenic 40.0%
! CCAATGGAGCATTTAGACGA+CGG - tig0031256:42141-42160 Msa1446030:three_prime_UTR 45.0%
TGAAGGCTGTCTCGAAAAGA+TGG - tig0031256:42289-42308 Msa1446030:CDS 45.0%
CATGGATCTATGCTTTGTGC+AGG - tig0031256:42373-42392 Msa1446030:CDS 45.0%
! CAGGATTTTCTTGACGAGGT+AGG + tig0031256:42435-42454 None:intergenic 45.0%
CTACCTCGTCAAGAAAATCC+TGG - tig0031256:42433-42452 Msa1446030:CDS 45.0%
! CTTCCAGGATTTTCTTGACG+AGG + tig0031256:42439-42458 None:intergenic 45.0%
ATCCTGGAAGCTCATTCATG+GGG - tig0031256:42449-42468 Msa1446030:CDS 45.0%
TTCCACCTCACGTCATGAAA+AGG - tig0031256:42472-42491 Msa1446030:CDS 45.0%
! TTCCTTTTCATGACGTGAGG+TGG + tig0031256:42477-42496 None:intergenic 45.0%
! CCTTTCCTTTTCATGACGTG+AGG + tig0031256:42480-42499 None:intergenic 45.0%
CCTCACGTCATGAAAAGGAA+AGG - tig0031256:42477-42496 Msa1446030:CDS 45.0%
GGTAGTGAGAATAGGGAAGA+AGG - tig0031256:43015-43034 Msa1446030:intron 45.0%
GAAAGGAACAAATGGAGGAG+TGG + tig0031256:43099-43118 None:intergenic 45.0%
! TTTGAAGCCTGCTAGGAAAG+TGG - tig0031256:43608-43627 Msa1446030:intron 45.0%
AAAGCCAAACCCACGTTTCA+AGG - tig0031256:43684-43703 Msa1446030:intron 45.0%
GAAAGTAGCTCCTTGAAACG+TGG + tig0031256:43697-43716 None:intergenic 45.0%
! GTTTCAAGGAGCTACTTTCG+TGG - tig0031256:43698-43717 Msa1446030:intron 45.0%
!!! TTCTAGCTTTTGCTGCAACG+TGG - tig0031256:43739-43758 Msa1446030:intron 45.0%
! CCCATGCTCTCTTGTTATCA+TGG - tig0031256:43829-43848 Msa1446030:intron 45.0%
TTCACAAGCAAAGTAGCACC+TGG + tig0031256:43984-44003 None:intergenic 45.0%
GATGTCCAACCATTTGTGGA+AGG + tig0031256:44085-44104 None:intergenic 45.0%
GTGAGATGTCCAACCATTTG+TGG + tig0031256:44089-44108 None:intergenic 45.0%
ACGATCCATCAAATGGCACA+TGG - tig0031256:44218-44237 Msa1446030:intron 45.0%
!! TGTTGCCATGTGCCATTTGA+TGG + tig0031256:44226-44245 None:intergenic 45.0%
ATTCCGTTGACAACAACTGC+AGG + tig0031256:44283-44302 None:intergenic 45.0%
CCAACTGCAACAAGTTTGCA+GGG + tig0031256:44412-44431 None:intergenic 45.0%
ACCAACTGCAACAAGTTTGC+AGG + tig0031256:44413-44432 None:intergenic 45.0%
AGCACTGTGGTTGTTGATGT+TGG - tig0031256:44463-44482 Msa1446030:CDS 45.0%
AAATATGGGAAGGGCGATAC+CGG - tig0031256:44495-44514 Msa1446030:CDS 45.0%
! GTCACAATTGGTGACAGGTT+TGG + tig0031256:44723-44742 None:intergenic 45.0%
GCATGGGACATTGTCACAAT+TGG + tig0031256:44735-44754 None:intergenic 45.0%
TGTTGAGGCACATAGATGTG+TGG - tig0031256:44806-44825 Msa1446030:five_prime_UTR 45.0%
!! TAAAAAAATATAAATATGAA+AGG + tig0031256:43116-43135 None:intergenic 5.0%
CCGTCGTCTAAATGCTCCAT+TGG + tig0031256:42144-42163 None:intergenic 50.0%
TCTGGAAGGCCACAAATCAG+TGG + tig0031256:42216-42235 None:intergenic 50.0%
AGGCTGTCTCGAAAAGATGG+AGG - tig0031256:42292-42311 Msa1446030:CDS 50.0%
GGCTGTCTCGAAAAGATGGA+GGG - tig0031256:42293-42312 Msa1446030:CDS 50.0%
CTCGAAAAGATGGAGGGACT+TGG - tig0031256:42299-42318 Msa1446030:CDS 50.0%
!! ACTTGGTTTGCAGCGAAGAG+TGG - tig0031256:42316-42335 Msa1446030:CDS 50.0%
AACCCCATGAATGAGCTTCC+AGG + tig0031256:42454-42473 None:intergenic 50.0%
CTCTTCTTATTGGGTGGCTG+TGG - tig0031256:42528-42547 Msa1446030:CDS 50.0%
CTGCTTCCTTGTCAACTGCT+AGG - tig0031256:42616-42635 Msa1446030:CDS 50.0%
! CTTCTGAAGGGAAAGTCGTG+AGG + tig0031256:42649-42668 None:intergenic 50.0%
GAGAACATGGGAAGCCAGTT+AGG + tig0031256:42819-42838 None:intergenic 50.0%
TGGAGGAGTGGAAGTTGACA+AGG + tig0031256:43087-43106 None:intergenic 50.0%
!! AGGTGTGTTTTGGGCGTGAA+GGG + tig0031256:43567-43586 None:intergenic 50.0%
!! TATCTGTTTGGGTTGGCGGT+GGG - tig0031256:43661-43680 Msa1446030:intron 50.0%
AGCTCCTTGAAACGTGGGTT+TGG + tig0031256:43691-43710 None:intergenic 50.0%
CAGACACCGACATGGTTTCA+GGG - tig0031256:44248-44267 Msa1446030:CDS 50.0%
TCTCCACCCTGAAACCATGT+CGG + tig0031256:44257-44276 None:intergenic 50.0%
GGTCCTGCAGTTGTTGTCAA+CGG - tig0031256:44277-44296 Msa1446030:CDS 50.0%
TGGATCAGAGTTCGGGTACT+AGG - tig0031256:44314-44333 Msa1446030:CDS 50.0%
!! CAGGGTGGTCTTGTAAGCAA+CGG + tig0031256:44394-44413 None:intergenic 50.0%
ACTGCAACAAGTTTGCAGGG+TGG + tig0031256:44409-44428 None:intergenic 50.0%
!! CCCTGCAAACTTGTTGCAGT+TGG - tig0031256:44409-44428 Msa1446030:CDS 50.0%
TGGAAAAGGGCTTAGCACTG+TGG - tig0031256:44450-44469 Msa1446030:CDS 50.0%
AATATGGGAAGGGCGATACC+GGG - tig0031256:44496-44515 Msa1446030:CDS 50.0%
TCTTATTGGGTGGCTGTGGC+TGG - tig0031256:42532-42551 Msa1446030:CDS 55.0%
!! CAGGTGTGTTTTGGGCGTGA+AGG + tig0031256:43568-43587 None:intergenic 55.0%
!! CTATCTGTTTGGGTTGGCGG+TGG - tig0031256:43660-43679 Msa1446030:intron 55.0%
GCTGCAACGTGGTTTCCTAC+CGG - tig0031256:43750-43769 Msa1446030:intron 55.0%
! AGAGAGCATGGGGCCAAAGT+AGG + tig0031256:43821-43840 None:intergenic 55.0%
GCAACATGCAGACACCGACA+TGG - tig0031256:44240-44259 Msa1446030:CDS 55.0%
GCAGACACCGACATGGTTTC+AGG - tig0031256:44247-44266 Msa1446030:CDS 55.0%
ACACCGACATGGTTTCAGGG+TGG - tig0031256:44251-44270 Msa1446030:CDS 55.0%
GACATGGTTTCAGGGTGGAG+AGG - tig0031256:44256-44275 Msa1446030:CDS 55.0%
! CGGGTACTAGGTTGACGATG+TGG - tig0031256:44326-44345 Msa1446030:CDS 55.0%
GGCATAAAGAGAGACGCGAG+TGG - tig0031256:44347-44366 Msa1446030:CDS 55.0%
AGAGAGACGCGAGTGGATAC+TGG - tig0031256:44354-44373 Msa1446030:CDS 55.0%
AGACGCGAGTGGATACTGGT+CGG - tig0031256:44358-44377 Msa1446030:CDS 55.0%
Chromosome Type Strat End Strand Name
tig0031256 gene 41977 44893 41977 ID=Msa1446030;Name=Msa1446030
tig0031256 mRNA 41977 44893 41977 ID=Msa1446030-mRNA-1;Parent=Msa1446030;Name=Msa1446030-mRNA-1;_AED=0.00;_eAED=0.00;_QI=122|1|1|1|1|1|2|311|378
tig0031256 exon 41977 42885 41977 ID=Msa1446030-mRNA-1:exon:15251;Parent=Msa1446030-mRNA-1
tig0031256 exon 44233 44893 44233 ID=Msa1446030-mRNA-1:exon:15250;Parent=Msa1446030-mRNA-1
tig0031256 five_prime_UTR 44772 44893 44772 ID=Msa1446030-mRNA-1:five_prime_utr;Parent=Msa1446030-mRNA-1
tig0031256 CDS 44233 44771 44233 ID=Msa1446030-mRNA-1:cds;Parent=Msa1446030-mRNA-1
tig0031256 CDS 42288 42885 42288 ID=Msa1446030-mRNA-1:cds;Parent=Msa1446030-mRNA-1
tig0031256 three_prime_UTR 41977 42287 41977 ID=Msa1446030-mRNA-1:three_prime_utr;Parent=Msa1446030-mRNA-1
Gene Sequence

>Msa1446030

ATGACATGTGTATTTTTATTTTTCAGGTCTTCTGAATTAATACCAATGGAGCATTTAGACGACGGCGAAGCATCAGAAAATAATGTTGATGAAGTAGAAGTCACTAATAATACTCCACTGATTTGTGGCCTTCCAGATGATATTTCTCTTTTGTGTCTGGCAAGAGTTCCTAGAAAATATCATTCGGTTTTGAAGGCTGTCTCGAAAAGATGGAGGGACTTGGTTTGCAGCGAAGAGTGGCTTTATTATCGTAGAAAGCATAAACTTGATGAGACATGGATCTATGCTTTGTGCAGGGATAAATTGGATCATGTTTACTGTTACGTTTTGGATCCTACCTCGTCAAGAAAATCCTGGAAGCTCATTCATGGGGTTCCACCTCACGTCATGAAAAGGAAAGGAATGGGTTTTGAAGCTTTGGGAAATAAGCTCTTCTTATTGGGTGGCTGTGGCTGGTCTGAAGATGCTACTGACGAAGTTTATTCATACAATGCTTCTTTGAACTCGTGGGTTGAAGCTGCTTCCTTGTCAACTGCTAGGTGCTACTTTGCTTGTGAAGCAATGGACGAAAAACTATATGCTATTGGTGGCATAGGTTCAAATTCAAGTGATCCTCATTCTTGGGATACTTTTGATCCTTCCACAAATGGTTGGACATCTCACAGAGATCCTAATATAGTTCCCGAAATTGAAGATTCAATAGTTATGGACGGCAAAATATACATCCGATGTGGTAAATCTCCTTTAACACCTCATGTGTATGCCGTTGTATACGATCCATCAAATGGCACATGGCAACATGCAGACACCGACATGGTTTCAGGGTGGAGAGGTCCTGCAGTTGTTGTCAACGGAATCCCTTTTGTTTTGGATCAGAGTTCGGGTACTAGGTTGACGATGTGGCATAAAGAGAGACGCGAGTGGATACTGGTCGGTAAGTTGTCACCGTTGCTTACAAGACCACCCTGCAAACTTGTTGCAGTTGGTAAAAGCATTTATATTATTGGAAAAGGGCTTAGCACTGTGGTTGTTGATGTTGGTGATATTGGAAATATGGGAAGGGCGATACCGGGTTCTTCTATACCGAAATTAGTCTCTGATCACAATGTAATTAGTTGTAAATGCTTGTCAATCTGA
Protein sequence

>Msa1446030

MTCVFLFFRSSELIPMEHLDDGEASENNVDEVEVTNNTPLICGLPDDISLLCLARVPRKYHSVLKAVSKRWRDLVCSEEWLYYRRKHKLDETWIYALCRDKLDHVYCYVLDPTSSRKSWKLIHGVPPHVMKRKGMGFEALGNKLFLLGGCGWSEDATDEVYSYNASLNSWVEAASLSTARCYFACEAMDEKLYAIGGIGSNSSDPHSWDTFDPSTNGWTSHRDPNIVPEIEDSIVMDGKIYIRCGKSPLTPHVYAVVYDPSNGTWQHADTDMVSGWRGPAVVVNGIPFVLDQSSGTRLTMWHKERREWILVGKLSPLLTRPPCKLVAVGKSIYIIGKGLSTVVVDVGDIGNMGRAIPGSSIPKLVSDHNVISCKCLSI*