AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1453070

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1453070 MtrunA17_Chr4g0038651 98.925 558 6 0 1 558 1 558 0.0 1143
Msa1453070 MtrunA17_Chr7g0240501 60.890 427 156 6 85 506 182 602 3.90e-176 512
Msa1453070 MtrunA17_Chr3g0112731 57.991 438 169 5 82 513 95 523 2.69e-175 508
Msa1453070 MtrunA17_Chr5g0436141 57.895 418 165 4 89 503 84 493 1.39e-167 488
Msa1453070 MtrunA17_Chr4g0008771 62.264 371 134 2 85 453 94 460 8.30e-160 466
Msa1453070 MtrunA17_Chr4g0004771 55.769 364 142 5 87 449 90 435 3.86e-140 416
Msa1453070 MtrunA17_Chr8g0348921 54.145 386 156 6 82 452 90 469 4.89e-138 408
Msa1453070 MtrunA17_Chr2g0331631 50.754 398 178 6 68 464 68 448 2.54e-133 399
Msa1453070 MtrunA17_Chr6g0485861 50.886 395 174 6 86 479 95 470 5.23e-132 395
Msa1453070 MtrunA17_Chr7g0247761 49.176 425 154 5 83 451 109 527 6.28e-127 382
Msa1453070 MtrunA17_Chr5g0408591 47.107 363 184 3 86 448 117 471 1.67e-111 343
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1453070 AT4G38430.1 69.947 569 139 13 1 558 1 548 0.0 740
Msa1453070 AT5G05940.1 57.143 448 179 6 81 518 96 540 4.88e-178 516
Msa1453070 AT5G02010.1 60.621 419 152 6 80 496 63 470 1.47e-177 512
Msa1453070 AT5G05940.2 61.290 341 126 2 81 416 96 435 2.34e-150 441
Msa1453070 AT3G55660.1 52.740 438 180 8 84 502 104 533 4.51e-148 438
Msa1453070 AT4G00460.2 55.764 373 152 6 87 452 104 470 3.72e-140 414
Msa1453070 AT4G00460.4 55.764 373 152 6 87 452 104 470 3.72e-140 414
Msa1453070 AT1G01700.1 54.301 372 159 5 87 452 116 482 1.80e-132 395
Msa1453070 AT4G13240.1 51.832 382 165 6 80 459 67 431 1.80e-130 391
Msa1453070 AT1G52240.1 55.096 363 140 6 88 448 105 446 6.56e-129 388
Msa1453070 AT3G24620.1 53.168 363 152 5 87 448 85 430 9.74e-129 387
Msa1453070 AT3G55660.2 55.425 341 137 5 84 416 104 437 1.05e-128 384
Msa1453070 AT1G79860.1 52.329 365 155 7 85 448 90 436 1.62e-128 386
Msa1453070 AT1G01700.4 50.249 402 159 6 87 452 116 512 5.57e-126 379
Msa1453070 AT5G19560.1 51.220 369 164 7 86 453 43 396 1.55e-120 365
Msa1453070 AT5G19560.5 51.220 369 164 7 86 453 43 396 1.55e-120 365
Msa1453070 AT2G45890.1 48.404 376 175 7 87 452 93 459 3.96e-120 362
Msa1453070 AT5G19560.4 51.220 369 164 7 86 453 62 415 4.72e-120 364
Msa1453070 AT4G00460.3 52.083 336 148 6 124 452 8 337 9.60e-116 347
Msa1453070 AT3G16130.1 49.176 364 169 5 86 448 89 437 4.90e-115 352
Msa1453070 AT1G01700.3 50.286 350 156 6 87 423 116 460 4.59e-109 334
Msa1453070 AT5G19560.2 49.398 332 152 7 86 416 62 378 2.81e-102 314
Msa1453070 AT1G31650.2 41.644 365 200 5 87 448 108 462 2.98e-96 304
Msa1453070 AT1G31650.1 41.644 365 200 5 87 448 136 490 1.38e-95 303
Msa1453070 AT4G00460.1 58.333 216 87 2 87 299 104 319 3.43e-82 260
Msa1453070 AT5G19560.3 49.097 277 125 7 178 453 5 266 1.64e-80 257
Msa1453070 AT1G01700.5 56.944 216 90 2 87 299 116 331 1.28e-78 252
Msa1453070 AT1G01700.2 56.944 216 90 2 87 299 116 331 1.28e-78 252

Find 206 sgRNAs with CRISPR-Local

Find 293 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTAATGCTGAATCTTAAAT+TGG 0.169189 tig0034461:-21255 None:intergenic
GATTCTGGCCATTATGTTAA+AGG 0.205615 tig0034461:-20453 None:intergenic
TCTAGAACTCAGTCTCTTTC+TGG 0.210377 tig0034461:-20815 None:intergenic
GATTGTGTTGGGTGATTCTA+AGG 0.220759 tig0034461:+19003 Msa1453070:CDS
ATAAGAGAAAATGAAATAAA+TGG 0.228648 tig0034461:-17845 None:intergenic
CAAATTGCTATGTAGAGTTT+TGG 0.254500 tig0034461:+21223 Msa1453070:three_prime_UTR
CCATGGCAGCCTTAAGTATC+TGG 0.261590 tig0034461:-19156 None:intergenic
CGACTGTGTTTGGTGAATTA+TGG 0.265683 tig0034461:+18723 Msa1453070:CDS
GATTCAAAAGAAATACATTA+TGG 0.273300 tig0034461:+21366 Msa1453070:three_prime_UTR
ATAGATACTAAGTGCCTCTT+TGG 0.277965 tig0034461:-20979 None:intergenic
CGAAGCCAACTTTCCGGCCT+TGG 0.278677 tig0034461:-20726 None:intergenic
AGCTTGAAACGCCGATTTCC+TGG 0.282493 tig0034461:+19864 Msa1453070:CDS
TTGTTCAGCAGGGGAGGTTT+TGG 0.286270 tig0034461:+20533 Msa1453070:CDS
ATCAGTCGAAGCCAACTTTC+CGG 0.286787 tig0034461:-20732 None:intergenic
TAGTTCTGTTCTTGCTGAAA+TGG 0.305090 tig0034461:+19186 Msa1453070:CDS
GGGGAAAGCCAGACGGTCTT+GGG 0.310784 tig0034461:+19764 Msa1453070:CDS
TAGAGAGAGCCGTTCATCTT+TGG 0.319016 tig0034461:+21027 Msa1453070:three_prime_UTR
CCTTCTGTGCAACAATTTCC+AGG 0.323902 tig0034461:+18839 Msa1453070:CDS
AACCCATTTGGTGGAAGCTT+TGG 0.329873 tig0034461:-19085 None:intergenic
CGGGGAAAGCCAGACGGTCT+TGG 0.332922 tig0034461:+19763 Msa1453070:CDS
GAGCTTAGCCTTTAACATAA+TGG 0.342778 tig0034461:+20445 Msa1453070:CDS
CGCCGCTCATTTCAACTTGC+CGG 0.344030 tig0034461:+18098 Msa1453070:CDS
TCTAAGGATTGTGATGATTA+TGG 0.346046 tig0034461:+19019 Msa1453070:CDS
TTAATTTGGTAACGCACATT+TGG 0.347361 tig0034461:-17886 None:intergenic
GATATGTCTGGTGGTGGAAA+AGG 0.352459 tig0034461:+18371 Msa1453070:CDS
TACCTTATTATACTGTATCT+TGG 0.354569 tig0034461:-19913 None:intergenic
GGCCAAGATACAGTATAATA+AGG 0.358263 tig0034461:+19911 Msa1453070:CDS
TGGGAAAGATGTTGCTGTTT+GGG 0.363508 tig0034461:+18152 Msa1453070:CDS
TTGCCGCAAGTTTGCAAAAC+GGG 0.372103 tig0034461:-20862 None:intergenic
TTGCTGCTGTTGTAAAGAAA+TGG 0.381868 tig0034461:+17924 Msa1453070:exon
AGCAATGTGGCGTAGGGAAA+TGG 0.382299 tig0034461:+18775 Msa1453070:CDS
TCTCCAAAGCTTCCACCAAA+TGG 0.384270 tig0034461:+19082 Msa1453070:CDS
AAAATTACACCCTCATATAC+AGG 0.387419 tig0034461:-21074 None:intergenic
AACACATTGAGACGCACGTT+TGG 0.387708 tig0034461:+20940 Msa1453070:three_prime_UTR
TGTACCATTCCCTGTATATG+AGG 0.388484 tig0034461:+21064 Msa1453070:three_prime_UTR
ATATGTCTGGTGGTGGAAAA+GGG 0.392050 tig0034461:+18372 Msa1453070:CDS
TAATTTGGTAACGCACATTT+GGG 0.399094 tig0034461:-17885 None:intergenic
CTCAGCGCAAGAAGAAATTC+TGG 0.399210 tig0034461:+20785 Msa1453070:CDS
TGTTCCTCCTCTCCTGTTAG+TGG 0.400070 tig0034461:+20644 Msa1453070:CDS
TACTAAGTGCCTCTTTGGTT+TGG 0.400178 tig0034461:-20974 None:intergenic
CTATTCTTGAAAGTTATTCC+AGG 0.402654 tig0034461:+20414 Msa1453070:CDS
TCCTCAAACAGCGCTAGATA+TGG 0.409212 tig0034461:+19890 Msa1453070:CDS
CTGCAAGGACTCCCACTAAC+AGG 0.411730 tig0034461:-20656 None:intergenic
GTGGGAAAGATGTTGCTGTT+TGG 0.414429 tig0034461:+18151 Msa1453070:CDS
ATAAAGGACATCATCGATTC+TGG 0.415180 tig0034461:-20468 None:intergenic
CAGATTCCATCTTGTTCAGC+AGG 0.416244 tig0034461:+20522 Msa1453070:CDS
CTTTCCACCCCAAGACCGTC+TGG 0.421418 tig0034461:-19772 None:intergenic
GACGGCGCAGGCTATGCTTC+CGG 0.425048 tig0034461:+18122 Msa1453070:CDS
TTCTAGAGTTGGCATGTGTT+TGG 0.426356 tig0034461:+20831 Msa1453070:three_prime_UTR
TAAGAGAAAATGAAATAAAT+GGG 0.430598 tig0034461:-17844 None:intergenic
AGCTTCTCTATTTCCACGTC+AGG 0.431442 tig0034461:+20917 Msa1453070:three_prime_UTR
TTATAGTATCGTCTACATAA+AGG 0.435898 tig0034461:-20484 None:intergenic
CCTGCATTGAAGAAGCTTGA+CGG 0.436172 tig0034461:+18920 Msa1453070:CDS
CTCTGTCAGTGTTGTTGTTG+AGG 0.439826 tig0034461:-17719 None:intergenic
ACACATTGAGACGCACGTTT+GGG 0.441992 tig0034461:+20941 Msa1453070:three_prime_UTR
TTTGAGGAAGCCCAGGAAAT+CGG 0.442800 tig0034461:-19875 None:intergenic
AGCTGAGCTGGAAGCTCCTT+CGG 0.443051 tig0034461:-18055 None:intergenic
CTCCAAAGCTTCCACCAAAT+GGG 0.444467 tig0034461:+19083 Msa1453070:CDS
AGCATAGCCTGCGCCGTCGC+CGG 0.445981 tig0034461:-18117 None:intergenic
TTGGGATCACAAACGTGACT+TGG 0.452605 tig0034461:+18170 Msa1453070:CDS
ATGGGTTGTGTGAGGATGAT+AGG 0.453326 tig0034461:+19101 Msa1453070:CDS
TCATCCTCACACAACCCATT+TGG 0.456886 tig0034461:-19097 None:intergenic
TGCAGAAGTTGAAATGATGA+AGG 0.459359 tig0034461:+18319 Msa1453070:intron
AGAATGGTAAAGCTTGTCTT+GGG 0.459633 tig0034461:+19580 Msa1453070:intron
GTAGCAAAAGGAGAGGCATA+TGG 0.460266 tig0034461:-20611 None:intergenic
GAACCACTTTCTTGTCCAAC+GGG 0.461497 tig0034461:+19824 Msa1453070:CDS
GTAATTGCAGCGACTGTGTT+TGG 0.462379 tig0034461:+18713 Msa1453070:intron
TGAATGTCTTCTTGACTGCC+TGG 0.463418 tig0034461:+19644 Msa1453070:CDS
CTGTTAGGCAAGAAGAGAAA+TGG 0.465548 tig0034461:+19050 Msa1453070:CDS
TCATTTCAACTTGCCGGCGA+CGG 0.465724 tig0034461:+18104 Msa1453070:CDS
CAACTTGCCGGCGACGGCGC+AGG 0.470587 tig0034461:+18110 Msa1453070:CDS
CAGAATGGTAAAGCTTGTCT+TGG 0.471373 tig0034461:+19579 Msa1453070:intron
CAGGCTATGCTTCCGGTCAT+CGG 0.475533 tig0034461:+18129 Msa1453070:CDS
GAAAGTTCTAGCAGCTTCTA+CGG 0.475680 tig0034461:+18018 Msa1453070:CDS
GTTGATAGAGGGATTGTGTT+GGG 0.477945 tig0034461:+18992 Msa1453070:CDS
ATTATGGTAGACCTTCTGTT+AGG 0.480466 tig0034461:+19035 Msa1453070:CDS
TGTTGAATTGAGAAGGGGCT+GGG 0.481257 tig0034461:-20584 None:intergenic
CCGTCAAGCTTCTTCAATGC+AGG 0.482835 tig0034461:-18920 None:intergenic
TGAAAGTTATTCCAGGGTGA+TGG 0.483592 tig0034461:+20421 Msa1453070:CDS
GTTCCTCCTCTCCTGTTAGT+GGG 0.489622 tig0034461:+20645 Msa1453070:CDS
TGCTGCTGTTGTAAAGAAAT+GGG 0.489934 tig0034461:+17925 Msa1453070:exon
GTGCAACAATTTCCAGGTGG+TGG 0.490405 tig0034461:+18845 Msa1453070:CDS
TGGCGAGAGATTAGCTGAGC+TGG 0.492120 tig0034461:-18067 None:intergenic
GCCATATCTAGCGCTGTTTG+AGG 0.492418 tig0034461:-19891 None:intergenic
AGATTCCAAGGCCGGAAAGT+TGG 0.493306 tig0034461:+20721 Msa1453070:CDS
GTTTGGTGAATTATGGAGGT+TGG 0.496317 tig0034461:+18730 Msa1453070:CDS
CCTGGAAATTGTTGCACAGA+AGG 0.500430 tig0034461:-18839 None:intergenic
CAGAATTTCTTCTTGCGCTG+AGG 0.503230 tig0034461:-20784 None:intergenic
TGTGTAGGCGTCATTGACTA+TGG 0.503238 tig0034461:-21301 None:intergenic
TTGAGGCTGCTATACATGTA+TGG 0.504433 tig0034461:+19709 Msa1453070:CDS
TAGCAAAAGGAGAGGCATAT+GGG 0.505390 tig0034461:-20610 None:intergenic
GTGTTGAATTGAGAAGGGGC+TGG 0.507650 tig0034461:-20585 None:intergenic
GTACCATTCCCTGTATATGA+GGG 0.509025 tig0034461:+21065 Msa1453070:three_prime_UTR
GCTTGAAACGCCGATTTCCT+GGG 0.512807 tig0034461:+19865 Msa1453070:CDS
TTCTCTTCTTGCCTAACAGA+AGG 0.513322 tig0034461:-19046 None:intergenic
CGGAATGCTGCTAAGTATGT+TGG 0.515803 tig0034461:+18940 Msa1453070:CDS
CTCCTTCGGCGTCGAAACTT+CGG 0.515808 tig0034461:-18041 None:intergenic
GGAGAGGATATGTCTGGTGG+TGG 0.518827 tig0034461:+18365 Msa1453070:CDS
CTAATGCTGAATCTTAAATT+GGG 0.519098 tig0034461:-21254 None:intergenic
CGACTGATTATGAGAAGGTA+TGG 0.520176 tig0034461:+20747 Msa1453070:CDS
CAGAACGAAGGAGTAGCAAA+AGG 0.523441 tig0034461:-20623 None:intergenic
CTATGGCAGTAAGGACATAA+TGG 0.524949 tig0034461:-21284 None:intergenic
TTGTACAAACCAGATACTTA+AGG 0.526700 tig0034461:+19147 Msa1453070:CDS
TCCATCTTGTTCAGCAGGGG+AGG 0.527658 tig0034461:+20527 Msa1453070:CDS
TCCTCACACAACCCATTTGG+TGG 0.531504 tig0034461:-19094 None:intergenic
AACGCCATCACGAATCTCTC+CGG 0.532591 tig0034461:+18419 Msa1453070:CDS
GCAGTAGTGTCTCTGCCCGT+TGG 0.535109 tig0034461:-19839 None:intergenic
TATGTCTGGTGGTGGAAAAG+GGG 0.535150 tig0034461:+18373 Msa1453070:CDS
TGCTGCTAAGTATGTTGGAT+GGG 0.536146 tig0034461:+18945 Msa1453070:CDS
ACCTCCCCTGCTGAACAAGA+TGG 0.537375 tig0034461:-20528 None:intergenic
TGACGTGGAAATAGAGAAGC+TGG 0.538221 tig0034461:-20915 None:intergenic
ACATCTTTCCCACCGATGAC+CGG 0.539491 tig0034461:-18141 None:intergenic
AAACGTGACTTGGATTTGAC+CGG 0.539763 tig0034461:+18180 Msa1453070:CDS
ATATACCGGTACATAACTGC+TGG 0.541260 tig0034461:+19609 Msa1453070:CDS
GGGGTGTGTTGAATTGAGAA+GGG 0.542741 tig0034461:-20590 None:intergenic
CGTCATTGACTATGGCAGTA+AGG 0.544352 tig0034461:-21293 None:intergenic
ATTGCCGCAAGTTTGCAAAA+CGG 0.546354 tig0034461:-20863 None:intergenic
TGTGGACCTGTGGAGTATAG+TGG 0.548211 tig0034461:-17696 None:intergenic
GAGTGCTGACATCAGCGAAT+CGG 0.550570 tig0034461:+17996 Msa1453070:CDS
AAGGGTAAAGATTCCAAGGC+CGG 0.550775 tig0034461:+20713 Msa1453070:CDS
TATTCTTGAAAGTTATTCCA+GGG 0.551588 tig0034461:+20415 Msa1453070:CDS
AAGGATTCAACATAAGCAGC+AGG 0.553157 tig0034461:-19214 None:intergenic
ACTTCATAAGTTCCACCACC+TGG 0.555405 tig0034461:-18857 None:intergenic
GCTATGCTTCCGGTCATCGG+TGG 0.557617 tig0034461:+18132 Msa1453070:CDS
AAATGAGCGGCGAGCTGACG+TGG 0.558695 tig0034461:-18087 None:intergenic
CCAGATACTTAAGGCTGCCA+TGG 0.558712 tig0034461:+19156 Msa1453070:CDS
TCAGTGTTGTTGTTGAGGTG+TGG 0.563762 tig0034461:-17714 None:intergenic
AAGCATTATTACCCTTGGCA+AGG 0.564336 tig0034461:-19233 None:intergenic
TGGTGGAACTTATGAAGTAA+TGG 0.566731 tig0034461:+18862 Msa1453070:CDS
GGTAACGCACATTTGGGTAA+CGG 0.567797 tig0034461:-17879 None:intergenic
AGCGCTGTTTGAGGAAGCCC+AGG 0.567802 tig0034461:-19882 None:intergenic
TGGGGTGTGTTGAATTGAGA+AGG 0.569446 tig0034461:-20591 None:intergenic
TATTCGTAGGCAATATGAGT+AGG 0.573733 tig0034461:+21101 Msa1453070:three_prime_UTR
AACAGAACTATTAATCGCCA+TGG 0.575102 tig0034461:-19173 None:intergenic
GTCTTGGGGATATAATATAC+CGG 0.575804 tig0034461:+19595 Msa1453070:CDS
AAAGCCAGACGGTCTTGGGG+TGG 0.577009 tig0034461:+19768 Msa1453070:CDS
ATTTGGAGGCATACCTGTGT+AGG 0.577249 tig0034461:-21316 None:intergenic
ACACCCTCATATACAGGGAA+TGG 0.577332 tig0034461:-21068 None:intergenic
ATAAAGCTTTCATGTTAACA+TGG 0.577730 tig0034461:-20889 None:intergenic
GTTGTTGAGGTGTGGACCTG+TGG 0.578794 tig0034461:-17706 None:intergenic
AGAACCACTTTCTTGTCCAA+CGG 0.580288 tig0034461:+19823 Msa1453070:CDS
TGTGTCCTGCGTGGACTGCA+AGG 0.582733 tig0034461:-20671 None:intergenic
TGTTGATAGAGGGATTGTGT+TGG 0.583213 tig0034461:+18991 Msa1453070:CDS
TAAAGCTTTCATGTTAACAT+GGG 0.584419 tig0034461:-20888 None:intergenic
CTGTGTTTGGTGAATTATGG+AGG 0.584849 tig0034461:+18726 Msa1453070:CDS
CTGCCCGTTGGACAAGAAAG+TGG 0.585462 tig0034461:-19827 None:intergenic
AAGAGACTGAGTTCTAGAGT+TGG 0.586872 tig0034461:+20820 Msa1453070:exon
AGATATAGCTAATAGAATTG+AGG 0.588400 tig0034461:+19692 Msa1453070:CDS
GAGTTGGCATGTGTTTGGAA+CGG 0.590639 tig0034461:+20836 Msa1453070:three_prime_UTR
GGCCGAAGTTTCGACGCCGA+AGG 0.592780 tig0034461:+18039 Msa1453070:CDS
TTTGGTGGAAGCTTTGGAGA+AGG 0.594961 tig0034461:-19079 None:intergenic
TATTAGCTATATCTTGTGTG+TGG 0.596055 tig0034461:-19683 None:intergenic
GCAGGACACATGATGTAAAG+AGG 0.599723 tig0034461:+20684 Msa1453070:CDS
CAGACGGTCTTGGGGTGGAA+AGG 0.600589 tig0034461:+19773 Msa1453070:CDS
CTTATGTTGAATCCTTGCCA+AGG 0.600693 tig0034461:+19221 Msa1453070:CDS
GAGTGATTCAATAGTCGAGT+TGG 0.601610 tig0034461:+18814 Msa1453070:CDS
GGGAAAGCCAGACGGTCTTG+GGG 0.602570 tig0034461:+19765 Msa1453070:CDS
CTATGCTTCCGGTCATCGGT+GGG 0.603684 tig0034461:+18133 Msa1453070:CDS
CATTGAGACGCACGTTTGGG+TGG 0.604175 tig0034461:+20944 Msa1453070:three_prime_UTR
AGATTCCATCTTGTTCAGCA+GGG 0.604566 tig0034461:+20523 Msa1453070:CDS
TTAGGAGAGGATATGTCTGG+TGG 0.604883 tig0034461:+18362 Msa1453070:CDS
GGTGCTCAGATGATAAATCC+AGG 0.607693 tig0034461:-19662 None:intergenic
ATGCTGCTAAGTATGTTGGA+TGG 0.608493 tig0034461:+18944 Msa1453070:CDS
TGGCAAGGGTAAAGATTCCA+AGG 0.610500 tig0034461:+20709 Msa1453070:CDS
AGAAATGGGAAGTGTTTCGT+CGG 0.610958 tig0034461:+17939 Msa1453070:exon
GGCTAAGCTCTCCATCACCC+TGG 0.611779 tig0034461:-20432 None:intergenic
GGCTTCGACTGATTATGAGA+AGG 0.612301 tig0034461:+20742 Msa1453070:CDS
TTATGTTGAATCCTTGCCAA+GGG 0.615016 tig0034461:+19222 Msa1453070:CDS
ACACATGATGTAAAGAGGAA+TGG 0.620963 tig0034461:+20689 Msa1453070:CDS
TGATGTAAAGAGGAATGGCA+AGG 0.621154 tig0034461:+20694 Msa1453070:CDS
GATTCCATCTTGTTCAGCAG+GGG 0.623013 tig0034461:+20524 Msa1453070:CDS
ACTGACCAGCAGTTATGTAC+CGG 0.623263 tig0034461:-19614 None:intergenic
GTCTCAATGTGTTCCTGACG+TGG 0.623749 tig0034461:-20930 None:intergenic
CGCAAGAAGAAATTCTGGCG+AGG 0.629857 tig0034461:+20790 Msa1453070:CDS
TCTTCTCACCATCAGCAACA+AGG 0.630373 tig0034461:-19803 None:intergenic
GTAAAGAGCCTTGTTGCTGA+TGG 0.632308 tig0034461:+19795 Msa1453070:CDS
GGGTGTGTTGAATTGAGAAG+GGG 0.632385 tig0034461:-20589 None:intergenic
AGCAAAAGGAGAGGCATATG+GGG 0.632893 tig0034461:-20609 None:intergenic
GATGTAAAGAGGAATGGCAA+GGG 0.633745 tig0034461:+20695 Msa1453070:CDS
TCACCACCACTATACTCCAC+AGG 0.634580 tig0034461:+17690 None:intergenic
GATTCGCTGATGTCAGCACT+CGG 0.634852 tig0034461:-17994 None:intergenic
GGAGTCCTTGCAGTCCACGC+AGG 0.635954 tig0034461:+20666 Msa1453070:CDS
ACTAGATAACCAAAGATGAA+CGG 0.641072 tig0034461:-21036 None:intergenic
ATAGTCAATGACGCCTACAC+AGG 0.642514 tig0034461:+21303 Msa1453070:three_prime_UTR
CGCCGGCAAGTTGAAATGAG+CGG 0.644525 tig0034461:-18100 None:intergenic
TGTGTGAGGATGATAGGAAA+AGG 0.644810 tig0034461:+19107 Msa1453070:CDS
ACTCCCACTAACAGGAGAGG+AGG 0.651034 tig0034461:-20648 None:intergenic
CGAAGGAGTAGCAAAAGGAG+AGG 0.653019 tig0034461:-20618 None:intergenic
TTAGGCAAGAAGAGAAATGG+TGG 0.655462 tig0034461:+19053 Msa1453070:CDS
TTTACATCATGTGTCCTGCG+TGG 0.655545 tig0034461:-20680 None:intergenic
TGTGGCGTAGGGAAATGGAA+TGG 0.660140 tig0034461:+18780 Msa1453070:CDS
GGAGAGGAGGAACAGAACGA+AGG 0.664755 tig0034461:-20635 None:intergenic
GGACCTGTGGAGTATAGTGG+TGG 0.667940 tig0034461:-17693 None:intergenic
GGTAGACTGCCAAACCAAAG+AGG 0.668978 tig0034461:+20965 Msa1453070:three_prime_UTR
AAATTACACCCTCATATACA+GGG 0.669557 tig0034461:-21073 None:intergenic
GAGAAGGTATGGTCATACGC+AGG 0.673120 tig0034461:+20758 Msa1453070:CDS
GAATGGTAAAGCTTGTCTTG+GGG 0.676070 tig0034461:+19581 Msa1453070:intron
CTAACCGGAGAGATTCGTGA+TGG 0.676096 tig0034461:-18423 None:intergenic
AGGACTCCCACTAACAGGAG+AGG 0.681765 tig0034461:-20651 None:intergenic
AGTAAGGACATAATGGCACA+TGG 0.682072 tig0034461:-21277 None:intergenic
TCCACCAAATGGGTTGTGTG+AGG 0.688832 tig0034461:+19093 Msa1453070:CDS
TCTGTGCAACAATTTCCAGG+TGG 0.694742 tig0034461:+18842 Msa1453070:CDS
GAAGATGTTTCAGAGCGATG+TGG 0.696488 tig0034461:+17961 Msa1453070:CDS
TTGATGTACAAATCAGAGCG+AGG 0.697670 tig0034461:-18893 None:intergenic
CTTATGAAGTAATGGCAACG+CGG 0.715804 tig0034461:+18870 Msa1453070:CDS
AGGCTGCTATACATGTATGG+AGG 0.750304 tig0034461:+19712 Msa1453070:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAGAGAAAATGAAATAAAT+GGG - tig0034461:17847-17866 None:intergenic 15.0%
!! ATAAGAGAAAATGAAATAAA+TGG - tig0034461:17848-17867 None:intergenic 15.0%
!!! AATTTTTTTCCTTCTAAAAT+TGG + tig0034461:19520-19539 Msa1453070:intron 15.0%
!! CTATAAGAAACTTAAAAAAT+CGG + tig0034461:19743-19762 Msa1453070:CDS 15.0%
!! TATAAGAAACTTAAAAAATC+GGG + tig0034461:19744-19763 Msa1453070:CDS 15.0%
!!! TTTTTTTTTTTTTTTTGCTG+AGG + tig0034461:20182-20201 Msa1453070:intron 15.0%
!!! AAATTTTGAGACTTAAAATT+TGG - tig0034461:21336-21355 None:intergenic 15.0%
!!! ATTTCTTTTGAATCAAAATA+TGG - tig0034461:21360-21379 None:intergenic 15.0%
!!! ATTTTACACAGATCTTAATT+TGG - tig0034461:17903-17922 None:intergenic 20.0%
!! ATGATTATATTGTGTTACAA+TGG + tig0034461:19327-19346 Msa1453070:intron 20.0%
!!! TGTAATTTTTGCATATTATG+TGG + tig0034461:19402-19421 Msa1453070:intron 20.0%
!!! TTATTAAAGTTTTTGAACCA+TGG - tig0034461:19453-19472 None:intergenic 20.0%
!! AAAACTTTAATAAGCTGAAA+TGG + tig0034461:19460-19479 Msa1453070:intron 20.0%
!! TTCAAAAATGGTTTAGTATT+TGG + tig0034461:19485-19504 Msa1453070:intron 20.0%
!! ATAAGAAACTTAAAAAATCG+GGG + tig0034461:19745-19764 Msa1453070:CDS 20.0%
!! ACAATGATCAATCATTATTA+CGG - tig0034461:20262-20281 None:intergenic 20.0%
!! AATCAAAGAATAAACTCATT+TGG + tig0034461:20359-20378 Msa1453070:intron 20.0%
!!! TGTAATTTTTGTATATTCGT+AGG + tig0034461:21088-21107 Msa1453070:three_prime_UTR 20.0%
!!! ATTTTTAATTGATGATCCAA+GGG - tig0034461:21146-21165 None:intergenic 20.0%
!!! TCCTTTCAAGTATAAATTTT+TGG + tig0034461:21169-21188 Msa1453070:three_prime_UTR 20.0%
!! TCCAAAAATTTATACTTGAA+AGG - tig0034461:21173-21192 None:intergenic 20.0%
!!! TTTCAAGTATAAATTTTTGG+AGG + tig0034461:21172-21191 Msa1453070:three_prime_UTR 20.0%
!! GATTCAAAAGAAATACATTA+TGG + tig0034461:21366-21385 Msa1453070:three_prime_UTR 20.0%
! AGCAAAAAAAACTAACTAAC+CGG - tig0034461:18441-18460 None:intergenic 25.0%
!! AGTTTTCATCCAATTTTAGA+AGG - tig0034461:19532-19551 None:intergenic 25.0%
!! GAAAACTTAAAATGCCTTTT+TGG + tig0034461:19545-19564 Msa1453070:intron 25.0%
! AGATATAGCTAATAGAATTG+AGG + tig0034461:19692-19711 Msa1453070:CDS 25.0%
! TACCTTATTATACTGTATCT+TGG - tig0034461:19916-19935 None:intergenic 25.0%
! TTTAGCAAAAGATATAATCC+TGG + tig0034461:20104-20123 Msa1453070:intron 25.0%
! AAAATTCTCTCACAAAATAC+AGG - tig0034461:20165-20184 None:intergenic 25.0%
! GAAAATACTGTTCATATGTT+AGG - tig0034461:20226-20245 None:intergenic 25.0%
! TATTCTTGAAAGTTATTCCA+GGG + tig0034461:20415-20434 Msa1453070:CDS 25.0%
! TTATAGTATCGTCTACATAA+AGG - tig0034461:20487-20506 None:intergenic 25.0%
! TAAAGCTTTCATGTTAACAT+GGG - tig0034461:20891-20910 None:intergenic 25.0%
! ATAAAGCTTTCATGTTAACA+TGG - tig0034461:20892-20911 None:intergenic 25.0%
!!! CATTTTTAATTGATGATCCA+AGG - tig0034461:21147-21166 None:intergenic 25.0%
! TTTATAGAAAAACACCATGT+GGG - tig0034461:21201-21220 None:intergenic 25.0%
! ATTTATAGAAAAACACCATG+TGG - tig0034461:21202-21221 None:intergenic 25.0%
! CTAATGCTGAATCTTAAATT+GGG - tig0034461:21257-21276 None:intergenic 25.0%
!!! TTTTGAGACTTAAAATTTGG+AGG - tig0034461:21333-21352 None:intergenic 25.0%
ACAAAGAAAAAGAATGTGAG+TGG + tig0034461:17769-17788 Msa1453070:five_prime_UTR 30.0%
!! TAATTTGGTAACGCACATTT+GGG - tig0034461:17888-17907 None:intergenic 30.0%
!! TTAATTTGGTAACGCACATT+TGG - tig0034461:17889-17908 None:intergenic 30.0%
AACATAAAGCCACAATTGTA+CGG - tig0034461:18237-18256 None:intergenic 30.0%
TTCTATGTAATCAGATGTGT+CGG + tig0034461:18540-18559 Msa1453070:intron 30.0%
ACATCTTCAATCAACATTGT+CGG + tig0034461:18591-18610 Msa1453070:intron 30.0%
!! AGTTTTGGTATGTTGATAGA+GGG + tig0034461:18981-19000 Msa1453070:CDS 30.0%
TCTAAGGATTGTGATGATTA+TGG + tig0034461:19019-19038 Msa1453070:CDS 30.0%
TTGTACAAACCAGATACTTA+AGG + tig0034461:19147-19166 Msa1453070:CDS 30.0%
TAATGCTTTGCTTTGCATAT+AGG + tig0034461:19248-19267 Msa1453070:intron 30.0%
TAGGAAATAAACTGTGTTCA+TGG + tig0034461:19267-19286 Msa1453070:intron 30.0%
! ACTGTGTTCATGGTTAATTT+TGG + tig0034461:19277-19296 Msa1453070:intron 30.0%
! CTGTGTTCATGGTTAATTTT+GGG + tig0034461:19278-19297 Msa1453070:intron 30.0%
GAATTGATGTCTGTCTAATA+TGG - tig0034461:19354-19373 None:intergenic 30.0%
GCTGAAATGGAATTCAAAAA+TGG + tig0034461:19473-19492 Msa1453070:intron 30.0%
TATTAGCTATATCTTGTGTG+TGG - tig0034461:19686-19705 None:intergenic 30.0%
! ATTTTCTCGCTCTTAAAGTT+TGG + tig0034461:19937-19956 Msa1453070:intron 30.0%
GAAGTGATATGCAGTATATT+AGG + tig0034461:20029-20048 Msa1453070:intron 30.0%
AAAAGATATAATCCTGGCAA+CGG + tig0034461:20110-20129 Msa1453070:intron 30.0%
TCTGTATGATAAGTTTGTGT+AGG + tig0034461:20285-20304 Msa1453070:intron 30.0%
TCTATTCTGTATAGTTACAG+AGG + tig0034461:20310-20329 Msa1453070:intron 30.0%
CTATTCTTGAAAGTTATTCC+AGG + tig0034461:20414-20433 Msa1453070:CDS 30.0%
ACTAGATAACCAAAGATGAA+CGG - tig0034461:21039-21058 None:intergenic 30.0%
AAATTACACCCTCATATACA+GGG - tig0034461:21076-21095 None:intergenic 30.0%
AAAATTACACCCTCATATAC+AGG - tig0034461:21077-21096 None:intergenic 30.0%
ATGAAGAAAAATTATGCCCT+TGG + tig0034461:21127-21146 Msa1453070:three_prime_UTR 30.0%
! CAAATTGCTATGTAGAGTTT+TGG + tig0034461:21223-21242 Msa1453070:three_prime_UTR 30.0%
GCTAATGCTGAATCTTAAAT+TGG - tig0034461:21258-21277 None:intergenic 30.0%
TTGCTGCTGTTGTAAAGAAA+TGG + tig0034461:17924-17943 Msa1453070:exon 35.0%
TGCTGCTGTTGTAAAGAAAT+GGG + tig0034461:17925-17944 Msa1453070:exon 35.0%
GAAACTGTGAAGTGTGTTAA+CGG + tig0034461:18283-18302 Msa1453070:intron 35.0%
TGCAGAAGTTGAAATGATGA+AGG + tig0034461:18319-18338 Msa1453070:intron 35.0%
GTGTTAGTAACTATGAAGCA+CGG + tig0034461:18467-18486 Msa1453070:intron 35.0%
TGGTGGAACTTATGAAGTAA+TGG + tig0034461:18862-18881 Msa1453070:CDS 35.0%
!! GGTTTTGTGATACACAGTTT+TGG + tig0034461:18966-18985 Msa1453070:CDS 35.0%
!! CAGTTTTGGTATGTTGATAG+AGG + tig0034461:18980-18999 Msa1453070:CDS 35.0%
ATTATGGTAGACCTTCTGTT+AGG + tig0034461:19035-19054 Msa1453070:CDS 35.0%
AACAGAACTATTAATCGCCA+TGG - tig0034461:19176-19195 None:intergenic 35.0%
! TAGTTCTGTTCTTGCTGAAA+TGG + tig0034461:19186-19205 Msa1453070:CDS 35.0%
TTATGTTGAATCCTTGCCAA+GGG + tig0034461:19222-19241 Msa1453070:CDS 35.0%
! AAGCAAAGCATTATTACCCT+TGG - tig0034461:19241-19260 None:intergenic 35.0%
!! GTTCATGGTTAATTTTGGGT+TGG + tig0034461:19282-19301 Msa1453070:intron 35.0%
!! GCTGAAATCAGTTTTATCCA+TGG + tig0034461:19433-19452 Msa1453070:intron 35.0%
! CTGCATTGGTAAAACCAAAA+AGG - tig0034461:19562-19581 None:intergenic 35.0%
!!! TTGGTTTTACCAATGCAGAA+TGG + tig0034461:19564-19583 Msa1453070:intron 35.0%
AGAATGGTAAAGCTTGTCTT+GGG + tig0034461:19580-19599 Msa1453070:intron 35.0%
GTCTTGGGGATATAATATAC+CGG + tig0034461:19595-19614 Msa1453070:CDS 35.0%
GGCCAAGATACAGTATAATA+AGG + tig0034461:19911-19930 Msa1453070:CDS 35.0%
ATGCAGTATATTAGGTGCAT+CGG + tig0034461:20037-20056 Msa1453070:intron 35.0%
TCTGTATAGTTACAGAGGTT+AGG + tig0034461:20315-20334 Msa1453070:intron 35.0%
! GAGCTTAGCCTTTAACATAA+TGG + tig0034461:20445-20464 Msa1453070:CDS 35.0%
GATTCTGGCCATTATGTTAA+AGG - tig0034461:20456-20475 None:intergenic 35.0%
!! ATAAAGGACATCATCGATTC+TGG - tig0034461:20471-20490 None:intergenic 35.0%
ACACATGATGTAAAGAGGAA+TGG + tig0034461:20689-20708 Msa1453070:CDS 35.0%
ATAGATACTAAGTGCCTCTT+TGG - tig0034461:20982-21001 None:intergenic 35.0%
TATTCGTAGGCAATATGAGT+AGG + tig0034461:21101-21120 Msa1453070:three_prime_UTR 35.0%
!! AGAAATGGGAAGTGTTTCGT+CGG + tig0034461:17939-17958 Msa1453070:exon 40.0%
! GAAAGTTCTAGCAGCTTCTA+CGG + tig0034461:18018-18037 Msa1453070:CDS 40.0%
!! TGGGAAAGATGTTGCTGTTT+GGG + tig0034461:18152-18171 Msa1453070:CDS 40.0%
! AAACGTGACTTGGATTTGAC+CGG + tig0034461:18180-18199 Msa1453070:CDS 40.0%
TATTCTCCTCCGTACAATTG+TGG + tig0034461:18225-18244 Msa1453070:intron 40.0%
ATAAAGCCACAATTGTACGG+AGG - tig0034461:18234-18253 None:intergenic 40.0%
!!! CGTTTTGCGAAGTTGCTTTT+AGG + tig0034461:18344-18363 Msa1453070:CDS 40.0%
!! CTTTTAGGAGAGGATATGTC+TGG + tig0034461:18359-18378 Msa1453070:CDS 40.0%
!! ATATGTCTGGTGGTGGAAAA+GGG + tig0034461:18372-18391 Msa1453070:CDS 40.0%
! CGACTGTGTTTGGTGAATTA+TGG + tig0034461:18723-18742 Msa1453070:CDS 40.0%
! CTGTGTTTGGTGAATTATGG+AGG + tig0034461:18726-18745 Msa1453070:CDS 40.0%
! GTTTGGTGAATTATGGAGGT+TGG + tig0034461:18730-18749 Msa1453070:CDS 40.0%
CACCACAGAAAAAAGCAATG+TGG + tig0034461:18762-18781 Msa1453070:CDS 40.0%
AGAAAAAAGCAATGTGGCGT+AGG + tig0034461:18768-18787 Msa1453070:CDS 40.0%
GAAAAAAGCAATGTGGCGTA+GGG + tig0034461:18769-18788 Msa1453070:CDS 40.0%
GAGTGATTCAATAGTCGAGT+TGG + tig0034461:18814-18833 Msa1453070:CDS 40.0%
CTTATGAAGTAATGGCAACG+CGG + tig0034461:18870-18889 Msa1453070:CDS 40.0%
TTGATGTACAAATCAGAGCG+AGG - tig0034461:18896-18915 None:intergenic 40.0%
ATGCTGCTAAGTATGTTGGA+TGG + tig0034461:18944-18963 Msa1453070:CDS 40.0%
TGCTGCTAAGTATGTTGGAT+GGG + tig0034461:18945-18964 Msa1453070:CDS 40.0%
! TGTTGATAGAGGGATTGTGT+TGG + tig0034461:18991-19010 Msa1453070:CDS 40.0%
! GTTGATAGAGGGATTGTGTT+GGG + tig0034461:18992-19011 Msa1453070:CDS 40.0%
GATTGTGTTGGGTGATTCTA+AGG + tig0034461:19003-19022 Msa1453070:CDS 40.0%
TTCTCTTCTTGCCTAACAGA+AGG - tig0034461:19049-19068 None:intergenic 40.0%
CTGTTAGGCAAGAAGAGAAA+TGG + tig0034461:19050-19069 Msa1453070:CDS 40.0%
TTAGGCAAGAAGAGAAATGG+TGG + tig0034461:19053-19072 Msa1453070:CDS 40.0%
TGTGTGAGGATGATAGGAAA+AGG + tig0034461:19107-19126 Msa1453070:CDS 40.0%
AAGGATTCAACATAAGCAGC+AGG - tig0034461:19217-19236 None:intergenic 40.0%
CTTATGTTGAATCCTTGCCA+AGG + tig0034461:19221-19240 Msa1453070:CDS 40.0%
AAGCATTATTACCCTTGGCA+AGG - tig0034461:19236-19255 None:intergenic 40.0%
CAAGCTTTACCATTCTGCAT+TGG - tig0034461:19576-19595 None:intergenic 40.0%
CAGAATGGTAAAGCTTGTCT+TGG + tig0034461:19579-19598 Msa1453070:intron 40.0%
GAATGGTAAAGCTTGTCTTG+GGG + tig0034461:19581-19600 Msa1453070:intron 40.0%
ATATACCGGTACATAACTGC+TGG + tig0034461:19609-19628 Msa1453070:CDS 40.0%
TTGAGGCTGCTATACATGTA+TGG + tig0034461:19709-19728 Msa1453070:CDS 40.0%
AGAACCACTTTCTTGTCCAA+CGG + tig0034461:19823-19842 Msa1453070:CDS 40.0%
TATATTAGGTGCATCGGATC+TGG + tig0034461:20043-20062 Msa1453070:intron 40.0%
CTCACAAAATACAGGCGTAA+GGG - tig0034461:20157-20176 None:intergenic 40.0%
TCTCACAAAATACAGGCGTA+AGG - tig0034461:20158-20177 None:intergenic 40.0%
TAGTTACAGAGGTTAGGCAT+CGG + tig0034461:20321-20340 Msa1453070:intron 40.0%
! TGGAACATTGTTTTCCTTGC+AGG + tig0034461:20379-20398 Msa1453070:intron 40.0%
! TTGTTTTCCTTGCAGGATGT+AGG + tig0034461:20386-20405 Msa1453070:intron 40.0%
! TGTTTTCCTTGCAGGATGTA+GGG + tig0034461:20387-20406 Msa1453070:intron 40.0%
TGAAAGTTATTCCAGGGTGA+TGG + tig0034461:20421-20440 Msa1453070:CDS 40.0%
AGATTCCATCTTGTTCAGCA+GGG + tig0034461:20523-20542 Msa1453070:CDS 40.0%
!! GGAGTCATTCGCTTTTGAAT+AGG - tig0034461:20566-20585 None:intergenic 40.0%
! TAGCAAAAGGAGAGGCATAT+GGG - tig0034461:20613-20632 None:intergenic 40.0%
TGATGTAAAGAGGAATGGCA+AGG + tig0034461:20694-20713 Msa1453070:CDS 40.0%
GATGTAAAGAGGAATGGCAA+GGG + tig0034461:20695-20714 Msa1453070:CDS 40.0%
CGACTGATTATGAGAAGGTA+TGG + tig0034461:20747-20766 Msa1453070:CDS 40.0%
! TCTAGAACTCAGTCTCTTTC+TGG - tig0034461:20818-20837 None:intergenic 40.0%
!! AAGAGACTGAGTTCTAGAGT+TGG + tig0034461:20820-20839 Msa1453070:exon 40.0%
!! TTCTAGAGTTGGCATGTGTT+TGG + tig0034461:20831-20850 Msa1453070:three_prime_UTR 40.0%
ATTGCCGCAAGTTTGCAAAA+CGG - tig0034461:20866-20885 None:intergenic 40.0%
TACTAAGTGCCTCTTTGGTT+TGG - tig0034461:20977-20996 None:intergenic 40.0%
TGTACCATTCCCTGTATATG+AGG + tig0034461:21064-21083 Msa1453070:three_prime_UTR 40.0%
GTACCATTCCCTGTATATGA+GGG + tig0034461:21065-21084 Msa1453070:three_prime_UTR 40.0%
ATAGAAAAACACCATGTGGG+TGG - tig0034461:21198-21217 None:intergenic 40.0%
AGTAAGGACATAATGGCACA+TGG - tig0034461:21280-21299 None:intergenic 40.0%
CTATGGCAGTAAGGACATAA+TGG - tig0034461:21287-21306 None:intergenic 40.0%
! TCAGTGTTGTTGTTGAGGTG+TGG - tig0034461:17717-17736 None:intergenic 45.0%
! CTCTGTCAGTGTTGTTGTTG+AGG - tig0034461:17722-17741 None:intergenic 45.0%
GGTAACGCACATTTGGGTAA+CGG - tig0034461:17882-17901 None:intergenic 45.0%
GAAGATGTTTCAGAGCGATG+TGG + tig0034461:17961-17980 Msa1453070:CDS 45.0%
! GTGGGAAAGATGTTGCTGTT+TGG + tig0034461:18151-18170 Msa1453070:CDS 45.0%
TTGGGATCACAAACGTGACT+TGG + tig0034461:18170-18189 Msa1453070:CDS 45.0%
TGAATGAGCGAGAACTCTAC+CGG - tig0034461:18202-18221 None:intergenic 45.0%
!!! TGCGAAGTTGCTTTTAGGAG+AGG + tig0034461:18349-18368 Msa1453070:CDS 45.0%
TTAGGAGAGGATATGTCTGG+TGG + tig0034461:18362-18381 Msa1453070:CDS 45.0%
!! GATATGTCTGGTGGTGGAAA+AGG + tig0034461:18371-18390 Msa1453070:CDS 45.0%
!! TATGTCTGGTGGTGGAAAAG+GGG + tig0034461:18373-18392 Msa1453070:CDS 45.0%
!! CAACATTGTCGGTGCTTTTG+AGG + tig0034461:18602-18621 Msa1453070:intron 45.0%
GTAATTGCAGCGACTGTGTT+TGG + tig0034461:18713-18732 Msa1453070:intron 45.0%
!!! GCTTTTTTCTGTGGTGCTAG+TGG - tig0034461:18758-18777 None:intergenic 45.0%
!!! CGCCACATTGCTTTTTTCTG+TGG - tig0034461:18767-18786 None:intergenic 45.0%
CCTGGAAATTGTTGCACAGA+AGG - tig0034461:18842-18861 None:intergenic 45.0%
CCTTCTGTGCAACAATTTCC+AGG + tig0034461:18839-18858 Msa1453070:CDS 45.0%
TCTGTGCAACAATTTCCAGG+TGG + tig0034461:18842-18861 Msa1453070:CDS 45.0%
ACTTCATAAGTTCCACCACC+TGG - tig0034461:18860-18879 None:intergenic 45.0%
CCTGCATTGAAGAAGCTTGA+CGG + tig0034461:18920-18939 Msa1453070:CDS 45.0%
CGGAATGCTGCTAAGTATGT+TGG + tig0034461:18940-18959 Msa1453070:CDS 45.0%
!! TTTGGTGGAAGCTTTGGAGA+AGG - tig0034461:19082-19101 None:intergenic 45.0%
TCTCCAAAGCTTCCACCAAA+TGG + tig0034461:19082-19101 Msa1453070:CDS 45.0%
CTCCAAAGCTTCCACCAAAT+GGG + tig0034461:19083-19102 Msa1453070:CDS 45.0%
! AACCCATTTGGTGGAAGCTT+TGG - tig0034461:19088-19107 None:intergenic 45.0%
TCATCCTCACACAACCCATT+TGG - tig0034461:19100-19119 None:intergenic 45.0%
! ATGGGTTGTGTGAGGATGAT+AGG + tig0034461:19101-19120 Msa1453070:CDS 45.0%
ACTGACCAGCAGTTATGTAC+CGG - tig0034461:19617-19636 None:intergenic 45.0%
TGAATGTCTTCTTGACTGCC+TGG + tig0034461:19644-19663 Msa1453070:CDS 45.0%
GGTGCTCAGATGATAAATCC+AGG - tig0034461:19665-19684 None:intergenic 45.0%
AGGCTGCTATACATGTATGG+AGG + tig0034461:19712-19731 Msa1453070:CDS 45.0%
AAAAATCGGGGAAAGCCAGA+CGG + tig0034461:19757-19776 Msa1453070:CDS 45.0%
!! GTAAAGAGCCTTGTTGCTGA+TGG + tig0034461:19795-19814 Msa1453070:CDS 45.0%
TCTTCTCACCATCAGCAACA+AGG - tig0034461:19806-19825 None:intergenic 45.0%
GAACCACTTTCTTGTCCAAC+GGG + tig0034461:19824-19843 Msa1453070:CDS 45.0%
TTTGAGGAAGCCCAGGAAAT+CGG - tig0034461:19878-19897 None:intergenic 45.0%
TCCTCAAACAGCGCTAGATA+TGG + tig0034461:19890-19909 Msa1453070:CDS 45.0%
TTGTTCTATGTACCGTTGCC+AGG - tig0034461:20125-20144 None:intergenic 45.0%
AGTTACAGAGGTTAGGCATC+GGG + tig0034461:20322-20341 Msa1453070:intron 45.0%
CAGATTCCATCTTGTTCAGC+AGG + tig0034461:20522-20541 Msa1453070:CDS 45.0%
GATTCCATCTTGTTCAGCAG+GGG + tig0034461:20524-20543 Msa1453070:CDS 45.0%
TGTTGAATTGAGAAGGGGCT+GGG - tig0034461:20587-20606 None:intergenic 45.0%
GGGTGTGTTGAATTGAGAAG+GGG - tig0034461:20592-20611 None:intergenic 45.0%
GGGGTGTGTTGAATTGAGAA+GGG - tig0034461:20593-20612 None:intergenic 45.0%
TGGGGTGTGTTGAATTGAGA+AGG - tig0034461:20594-20613 None:intergenic 45.0%
! AGCAAAAGGAGAGGCATATG+GGG - tig0034461:20612-20631 None:intergenic 45.0%
! GTAGCAAAAGGAGAGGCATA+TGG - tig0034461:20614-20633 None:intergenic 45.0%
CAGAACGAAGGAGTAGCAAA+AGG - tig0034461:20626-20645 None:intergenic 45.0%
TTTACATCATGTGTCCTGCG+TGG - tig0034461:20683-20702 None:intergenic 45.0%
GCAGGACACATGATGTAAAG+AGG + tig0034461:20684-20703 Msa1453070:CDS 45.0%
TGGCAAGGGTAAAGATTCCA+AGG + tig0034461:20709-20728 Msa1453070:CDS 45.0%
AAGGGTAAAGATTCCAAGGC+CGG + tig0034461:20713-20732 Msa1453070:CDS 45.0%
ATCAGTCGAAGCCAACTTTC+CGG - tig0034461:20735-20754 None:intergenic 45.0%
GGCTTCGACTGATTATGAGA+AGG + tig0034461:20742-20761 Msa1453070:CDS 45.0%
CAGAATTTCTTCTTGCGCTG+AGG - tig0034461:20787-20806 None:intergenic 45.0%
CTCAGCGCAAGAAGAAATTC+TGG + tig0034461:20785-20804 Msa1453070:CDS 45.0%
GAGTTGGCATGTGTTTGGAA+CGG + tig0034461:20836-20855 Msa1453070:three_prime_UTR 45.0%
TTGCCGCAAGTTTGCAAAAC+GGG - tig0034461:20865-20884 None:intergenic 45.0%
TGACGTGGAAATAGAGAAGC+TGG - tig0034461:20918-20937 None:intergenic 45.0%
AGCTTCTCTATTTCCACGTC+AGG + tig0034461:20917-20936 Msa1453070:three_prime_UTR 45.0%
AACACATTGAGACGCACGTT+TGG + tig0034461:20940-20959 Msa1453070:three_prime_UTR 45.0%
ACACATTGAGACGCACGTTT+GGG + tig0034461:20941-20960 Msa1453070:three_prime_UTR 45.0%
TAGAGAGAGCCGTTCATCTT+TGG + tig0034461:21027-21046 Msa1453070:three_prime_UTR 45.0%
ACACCCTCATATACAGGGAA+TGG - tig0034461:21071-21090 None:intergenic 45.0%
! CGTCATTGACTATGGCAGTA+AGG - tig0034461:21296-21315 None:intergenic 45.0%
! TGTGTAGGCGTCATTGACTA+TGG - tig0034461:21304-21323 None:intergenic 45.0%
ATAGTCAATGACGCCTACAC+AGG + tig0034461:21303-21322 Msa1453070:three_prime_UTR 45.0%
! ATTTGGAGGCATACCTGTGT+AGG - tig0034461:21319-21338 None:intergenic 45.0%
TGTGGACCTGTGGAGTATAG+TGG - tig0034461:17699-17718 None:intergenic 50.0%
GATTCGCTGATGTCAGCACT+CGG - tig0034461:17997-18016 None:intergenic 50.0%
!! GAGTGCTGACATCAGCGAAT+CGG + tig0034461:17996-18015 Msa1453070:CDS 50.0%
TCATTTCAACTTGCCGGCGA+CGG + tig0034461:18104-18123 Msa1453070:CDS 50.0%
ACATCTTTCCCACCGATGAC+CGG - tig0034461:18144-18163 None:intergenic 50.0%
AACGCCATCACGAATCTCTC+CGG + tig0034461:18419-18438 Msa1453070:CDS 50.0%
CTAACCGGAGAGATTCGTGA+TGG - tig0034461:18426-18445 None:intergenic 50.0%
AGCAATGTGGCGTAGGGAAA+TGG + tig0034461:18775-18794 Msa1453070:CDS 50.0%
TGTGGCGTAGGGAAATGGAA+TGG + tig0034461:18780-18799 Msa1453070:CDS 50.0%
GTGCAACAATTTCCAGGTGG+TGG + tig0034461:18845-18864 Msa1453070:CDS 50.0%
CCGTCAAGCTTCTTCAATGC+AGG - tig0034461:18923-18942 None:intergenic 50.0%
!! TCCACCAAATGGGTTGTGTG+AGG + tig0034461:19093-19112 Msa1453070:CDS 50.0%
TCCTCACACAACCCATTTGG+TGG - tig0034461:19097-19116 None:intergenic 50.0%
CCATGGCAGCCTTAAGTATC+TGG - tig0034461:19159-19178 None:intergenic 50.0%
CCAGATACTTAAGGCTGCCA+TGG + tig0034461:19156-19175 Msa1453070:CDS 50.0%
!! AGCTTGAAACGCCGATTTCC+TGG + tig0034461:19864-19883 Msa1453070:CDS 50.0%
!! GCTTGAAACGCCGATTTCCT+GGG + tig0034461:19865-19884 Msa1453070:CDS 50.0%
GCCATATCTAGCGCTGTTTG+AGG - tig0034461:19894-19913 None:intergenic 50.0%
! TTGTTCAGCAGGGGAGGTTT+TGG + tig0034461:20533-20552 Msa1453070:CDS 50.0%
GTGTTGAATTGAGAAGGGGC+TGG - tig0034461:20588-20607 None:intergenic 50.0%
CGAAGGAGTAGCAAAAGGAG+AGG - tig0034461:20621-20640 None:intergenic 50.0%
TGTTCCTCCTCTCCTGTTAG+TGG + tig0034461:20644-20663 Msa1453070:CDS 50.0%
GTTCCTCCTCTCCTGTTAGT+GGG + tig0034461:20645-20664 Msa1453070:CDS 50.0%
AGATTCCAAGGCCGGAAAGT+TGG + tig0034461:20721-20740 Msa1453070:CDS 50.0%
! GAGAAGGTATGGTCATACGC+AGG + tig0034461:20758-20777 Msa1453070:CDS 50.0%
CGCAAGAAGAAATTCTGGCG+AGG + tig0034461:20790-20809 Msa1453070:CDS 50.0%
! CGTCCCGTTTTGCAAACTTG+CGG + tig0034461:20859-20878 Msa1453070:three_prime_UTR 50.0%
GTCTCAATGTGTTCCTGACG+TGG - tig0034461:20933-20952 None:intergenic 50.0%
GGTAGACTGCCAAACCAAAG+AGG + tig0034461:20965-20984 Msa1453070:three_prime_UTR 50.0%
!! ATTTTTGGAGGCCACCCACA+TGG + tig0034461:21184-21203 Msa1453070:three_prime_UTR 50.0%
GTTGTTGAGGTGTGGACCTG+TGG - tig0034461:17709-17728 None:intergenic 55.0%
! CTCCTTCGGCGTCGAAACTT+CGG - tig0034461:18044-18063 None:intergenic 55.0%
AGCTGAGCTGGAAGCTCCTT+CGG - tig0034461:18058-18077 None:intergenic 55.0%
! TGGCGAGAGATTAGCTGAGC+TGG - tig0034461:18070-18089 None:intergenic 55.0%
CGCCGCTCATTTCAACTTGC+CGG + tig0034461:18098-18117 Msa1453070:CDS 55.0%
! CGCCGGCAAGTTGAAATGAG+CGG - tig0034461:18103-18122 None:intergenic 55.0%
CAGGCTATGCTTCCGGTCAT+CGG + tig0034461:18129-18148 Msa1453070:CDS 55.0%
CTATGCTTCCGGTCATCGGT+GGG + tig0034461:18133-18152 Msa1453070:CDS 55.0%
GGAGAGGATATGTCTGGTGG+TGG + tig0034461:18365-18384 Msa1453070:CDS 55.0%
CTGCCCGTTGGACAAGAAAG+TGG - tig0034461:19830-19849 None:intergenic 55.0%
!! AGAGGTTAGGCATCGGGAGA+AGG + tig0034461:20328-20347 Msa1453070:intron 55.0%
!! GAGGTTAGGCATCGGGAGAA+GGG + tig0034461:20329-20348 Msa1453070:intron 55.0%
AGACTGCCCTACATCCTGCA+AGG - tig0034461:20396-20415 None:intergenic 55.0%
TCCATCTTGTTCAGCAGGGG+AGG + tig0034461:20527-20546 Msa1453070:CDS 55.0%
ACCTCCCCTGCTGAACAAGA+TGG - tig0034461:20531-20550 None:intergenic 55.0%
!! TTCAGCAGGGGAGGTTTTGG+TGG + tig0034461:20536-20555 Msa1453070:CDS 55.0%
GGAGAGGAGGAACAGAACGA+AGG - tig0034461:20638-20657 None:intergenic 55.0%
ACTCCCACTAACAGGAGAGG+AGG - tig0034461:20651-20670 None:intergenic 55.0%
AGGACTCCCACTAACAGGAG+AGG - tig0034461:20654-20673 None:intergenic 55.0%
CTGCAAGGACTCCCACTAAC+AGG - tig0034461:20659-20678 None:intergenic 55.0%
CATTGAGACGCACGTTTGGG+TGG + tig0034461:20944-20963 Msa1453070:three_prime_UTR 55.0%
AAATGAGCGGCGAGCTGACG+TGG - tig0034461:18090-18109 None:intergenic 60.0%
GCTATGCTTCCGGTCATCGG+TGG + tig0034461:18132-18151 Msa1453070:CDS 60.0%
GGGGAAAGCCAGACGGTCTT+GGG + tig0034461:19764-19783 Msa1453070:CDS 60.0%
GGGAAAGCCAGACGGTCTTG+GGG + tig0034461:19765-19784 Msa1453070:CDS 60.0%
AAAGCCAGACGGTCTTGGGG+TGG + tig0034461:19768-19787 Msa1453070:CDS 60.0%
CTTTCCACCCCAAGACCGTC+TGG - tig0034461:19775-19794 None:intergenic 60.0%
CAGACGGTCTTGGGGTGGAA+AGG + tig0034461:19773-19792 Msa1453070:CDS 60.0%
GCAGTAGTGTCTCTGCCCGT+TGG - tig0034461:19842-19861 None:intergenic 60.0%
AGCGCTGTTTGAGGAAGCCC+AGG - tig0034461:19885-19904 None:intergenic 60.0%
GGCTAAGCTCTCCATCACCC+TGG - tig0034461:20435-20454 None:intergenic 60.0%
! TGTGTCCTGCGTGGACTGCA+AGG - tig0034461:20674-20693 None:intergenic 60.0%
CGAAGCCAACTTTCCGGCCT+TGG - tig0034461:20729-20748 None:intergenic 60.0%
GGCCGAAGTTTCGACGCCGA+AGG + tig0034461:18039-18058 Msa1453070:CDS 65.0%
GACGGCGCAGGCTATGCTTC+CGG + tig0034461:18122-18141 Msa1453070:CDS 65.0%
CGGGGAAAGCCAGACGGTCT+TGG + tig0034461:19763-19782 Msa1453070:CDS 65.0%
GGAGTCCTTGCAGTCCACGC+AGG + tig0034461:20666-20685 Msa1453070:CDS 65.0%
AGCATAGCCTGCGCCGTCGC+CGG - tig0034461:18120-18139 None:intergenic 70.0%
CAACTTGCCGGCGACGGCGC+AGG + tig0034461:18110-18129 Msa1453070:CDS 75.0%
Chromosome Type Strat End Strand Name
tig0034461 gene 17696 21445 17696 ID=Msa1453070;Name=Msa1453070
tig0034461 mRNA 17696 21445 17696 ID=Msa1453070-mRNA-1;Parent=Msa1453070;Name=Msa1453070-mRNA-1;_AED=0.06;_eAED=0.06;_QI=247|1|1|1|1|1|5|616|558
tig0034461 exon 17696 18201 17696 ID=Msa1453070-mRNA-1:exon:18973;Parent=Msa1453070-mRNA-1
tig0034461 exon 18324 18440 18324 ID=Msa1453070-mRNA-1:exon:18974;Parent=Msa1453070-mRNA-1
tig0034461 exon 18723 19243 18723 ID=Msa1453070-mRNA-1:exon:18975;Parent=Msa1453070-mRNA-1
tig0034461 exon 19582 19932 19582 ID=Msa1453070-mRNA-1:exon:18976;Parent=Msa1453070-mRNA-1
tig0034461 exon 20401 21445 20401 ID=Msa1453070-mRNA-1:exon:18977;Parent=Msa1453070-mRNA-1
tig0034461 five_prime_UTR 17696 17942 17696 ID=Msa1453070-mRNA-1:five_prime_utr;Parent=Msa1453070-mRNA-1
tig0034461 CDS 17943 18201 17943 ID=Msa1453070-mRNA-1:cds;Parent=Msa1453070-mRNA-1
tig0034461 CDS 18324 18440 18324 ID=Msa1453070-mRNA-1:cds;Parent=Msa1453070-mRNA-1
tig0034461 CDS 18723 19243 18723 ID=Msa1453070-mRNA-1:cds;Parent=Msa1453070-mRNA-1
tig0034461 CDS 19582 19932 19582 ID=Msa1453070-mRNA-1:cds;Parent=Msa1453070-mRNA-1
tig0034461 CDS 20401 20829 20401 ID=Msa1453070-mRNA-1:cds;Parent=Msa1453070-mRNA-1
tig0034461 three_prime_UTR 20830 21445 20830 ID=Msa1453070-mRNA-1:three_prime_utr;Parent=Msa1453070-mRNA-1
Gene Sequence

>Msa1453070

ATGGGAAGTGTTTCGTCGGAAGATGTTTCAGAGCGATGTGGTAGTTACAGTCCGAGTGCTGACATCAGCGAATCGGAAAGTTCTAGCAGCTTCTACGGCCGAAGTTTCGACGCCGAAGGAGCTTCCAGCTCAGCTAATCTCTCGCCACGTCAGCTCGCCGCTCATTTCAACTTGCCGGCGACGGCGCAGGCTATGCTTCCGGTCATCGGTGGGAAAGATGTTGCTGTTTGGGATCACAAACGTGACTTGGATTTGACCGAAGTTGAAATGATGAAGGAACGTTTTGCGAAGTTGCTTTTAGGAGAGGATATGTCTGGTGGTGGAAAAGGGGTTTGCACTGCACTTGCAATCTCTAACGCCATCACGAATCTCTCCGCGACTGTGTTTGGTGAATTATGGAGGTTGGAGCCACTAGCACCACAGAAAAAAGCAATGTGGCGTAGGGAAATGGAATGGCTACTTTGCGTGAGTGATTCAATAGTCGAGTTGGTGCCTTCTGTGCAACAATTTCCAGGTGGTGGAACTTATGAAGTAATGGCAACGCGGCCTCGCTCTGATTTGTACATCAATCTTCCTGCATTGAAGAAGCTTGACGGAATGCTGCTAAGTATGTTGGATGGGTTTTGTGATACACAGTTTTGGTATGTTGATAGAGGGATTGTGTTGGGTGATTCTAAGGATTGTGATGATTATGGTAGACCTTCTGTTAGGCAAGAAGAGAAATGGTGGCTTCCTTCTCCAAAGCTTCCACCAAATGGGTTGTGTGAGGATGATAGGAAAAGGTTGCAGCAGTGTAGAGATTGTACAAACCAGATACTTAAGGCTGCCATGGCGATTAATAGTTCTGTTCTTGCTGAAATGGAAATTCCTGCTGCTTATGTTGAATCCTTGCCAAGGAATGGTAAAGCTTGTCTTGGGGATATAATATACCGGTACATAACTGCTGGTCAGTTTTCGTCTGAATGTCTTCTTGACTGCCTGGATTTATCATCTGAGCACCACACACAAGATATAGCTAATAGAATTGAGGCTGCTATACATGTATGGAGGTTGAAAGACTATAAGAAACTTAAAAAATCGGGGAAAGCCAGACGGTCTTGGGGTGGAAAGGTAAAGAGCCTTGTTGCTGATGGTGAGAAGAACCACTTTCTTGTCCAACGGGCAGAGACACTACTGCAAAGCTTGAAACGCCGATTTCCTGGGCTTCCTCAAACAGCGCTAGATATGGCCAAGATACAGTATAATAAGGATGTAGGGCAGTCTATTCTTGAAAGTTATTCCAGGGTGATGGAGAGCTTAGCCTTTAACATAATGGCCAGAATCGATGATGTCCTTTATGTAGACGATACTATAAAACGATGTGCAGCTGCAGATTCCATCTTGTTCAGCAGGGGAGGTTTTGGTGGTCTGCCTATTCAAAAGCGAATGACTCCCAGCCCCTTCTCAATTCAACACACCCCATATGCCTCTCCTTTTGCTACTCCTTCGTTCTGTTCCTCCTCTCCTGTTAGTGGGAGTCCTTGCAGTCCACGCAGGACACATGATGTAAAGAGGAATGGCAAGGGTAAAGATTCCAAGGCCGGAAAGTTGGCTTCGACTGATTATGAGAAGGTATGGTCATACGCAGGAAACCTCAGCGCAAGAAGAAATTCTGGCGAGGCTCCAGAAAGAGACTGA
Protein sequence

>Msa1453070

MGSVSSEDVSERCGSYSPSADISESESSSSFYGRSFDAEGASSSANLSPRQLAAHFNLPATAQAMLPVIGGKDVAVWDHKRDLDLTEVEMMKERFAKLLLGEDMSGGGKGVCTALAISNAITNLSATVFGELWRLEPLAPQKKAMWRREMEWLLCVSDSIVELVPSVQQFPGGGTYEVMATRPRSDLYINLPALKKLDGMLLSMLDGFCDTQFWYVDRGIVLGDSKDCDDYGRPSVRQEEKWWLPSPKLPPNGLCEDDRKRLQQCRDCTNQILKAAMAINSSVLAEMEIPAAYVESLPRNGKACLGDIIYRYITAGQFSSECLLDCLDLSSEHHTQDIANRIEAAIHVWRLKDYKKLKKSGKARRSWGGKVKSLVADGEKNHFLVQRAETLLQSLKRRFPGLPQTALDMAKIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDTIKRCAAADSILFSRGGFGGLPIQKRMTPSPFSIQHTPYASPFATPSFCSSSPVSGSPCSPRRTHDVKRNGKGKDSKAGKLASTDYEKVWSYAGNLSARRNSGEAPERD*