AlfalfaGEDB | Msa1460630

M. sativa cultivar zhongmu-4 / Msa1460630

KEGG: -
GO: GO:0000003 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006457 GO:0006996 GO:0007275 GO:0008150 GO:0008219 GO:0009314 GO:0009416 GO:0009507 GO:0009526 GO:0009528 GO:0009532 GO:0009534 GO:0009536 GO:0009570 GO:0009579 GO:0009628 GO:0009639 GO:0009640 GO:0009657 GO:0009658 GO:0009704 GO:0009706 GO:0009790 GO:0009791 GO:0009793 GO:0009941 GO:0009987 GO:0010154 GO:0016020 GO:0016043 GO:0019866 GO:0022414 GO:0031090 GO:0031967 GO:0031969 GO:0031975 GO:0031976 GO:0031984 GO:0032386 GO:0032388 GO:0032501 GO:0032502 GO:0032879 GO:0032880 GO:0033157 GO:0034357 GO:0034762 GO:0034764 GO:0042170 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044183 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0048316 GO:0048518 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050896 GO:0051049 GO:0051050 GO:0051222 GO:0051223 GO:0055035 GO:0060341 GO:0061077 GO:0061458 GO:0065007 GO:0070201 GO:0071840 GO:0090087 GO:0090316 GO:1903533 GO:1903827 GO:1903829 GO:1904215 GO:1904216 GO:1904589 GO:1904591 GO:1904951
PFAM: PF11833.11

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1460630	ACJ83698.1	100.000	76	0	0	16	91	11	86	5.75e-46	152

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1460630	sp\|Q9FN50\|CPP1_ARATH	73.684	76	20	0	16	91	183	258	7.50e-33	117

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1460630	B7FG75	100.000	76	0	0	16	91	11	86	2.75e-46	152

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0064380	Msa1460630	0.813658	2.289338e-51	-8.615850e-47
Msa0090770	Msa1460630	-0.816136	6.461578e-52	-8.615850e-47
Msa1361100	Msa1460630	0.821961	3.056653e-53	-8.615850e-47
Msa1460630	Msa1465660	0.853473	2.335558e-61	-8.615850e-47
Msa0766100	Msa1460630	-0.807229	5.598067e-50	-8.615850e-47
Msa0183640	Msa1460630	-0.802463	5.543952e-49	-8.615850e-47
Msa0376450	Msa1460630	-0.804244	2.370985e-49	-8.615850e-47
Msa0471720	Msa1460630	-0.803505	3.376972e-49	-8.615850e-47
Msa1076570	Msa1460630	-0.806040	9.977967e-50	-8.615850e-47
Msa1083150	Msa1460630	0.809560	1.781017e-50	-8.615850e-47
Msa0312650	Msa1460630	0.848284	6.760429e-60	-8.615850e-47
Msa0516510	Msa1460630	-0.806052	9.917272e-50	-8.615850e-47
Msa0967650	Msa1460630	-0.802001	6.901580e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1460630	MtrunA17_Chr4g0062111	100.000	76	0	0	16	91	176	251	1.98e-48	154

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1460630	AT5G23040.2	73.684	76	20	0	16	91	183	258	7.62e-34	117
Msa1460630	AT5G23040.1	73.684	76	20	0	16	91	183	258	7.62e-34	117

Find 24 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACATTATTGAAAAGATAATT+TGG	0.217194	tig0041007:-16129	Msa1460630:five_prime_UTR
CAGCAACATTGTTGGAATAT+TGG	0.219376	tig0041007:+15833	None:intergenic
TTGGCTAGAGCGTTCGTTAT+TGG	0.284075	tig0041007:-15985	Msa1460630:CDS
GCTACAGGTGGCAATCTCTT+TGG	0.284234	tig0041007:-16046	Msa1460630:CDS
TGTACTCTTAATGAGTTTAT+AGG	0.296618	tig0041007:-15692	None:intergenic
GTTGCCACTTCACTTGAAAA+AGG	0.300132	tig0041007:+15743	None:intergenic
AGCAACATTGTTGGAATATT+GGG	0.319394	tig0041007:+15834	None:intergenic
TGGCTAGAGCGTTCGTTATT+GGG	0.329181	tig0041007:-15984	Msa1460630:intron
ATGGGTCTCTGGTTCATTGC+TGG	0.351564	tig0041007:-15859	Msa1460630:CDS
CTTGTGGCTGGATGGGTCTC+TGG	0.370387	tig0041007:-15870	Msa1460630:CDS
AAATGAAAAGACAAAGAGTT+TGG	0.389051	tig0041007:-16004	Msa1460630:CDS
AGGTTTGGAGCTCTTGTGGC+TGG	0.394881	tig0041007:-15882	Msa1460630:CDS
TTGGAGCTCTTGTGGCTGGA+TGG	0.399967	tig0041007:-15878	Msa1460630:CDS
TGGAGCTCTTGTGGCTGGAT+GGG	0.401053	tig0041007:-15877	Msa1460630:CDS
CCAACGATGTTAAGAGTTCA+AGG	0.419073	tig0041007:+15796	None:intergenic
CAATGTTGCTGCGCCCAACT+TGG	0.429282	tig0041007:-15821	Msa1460630:CDS
CAGTACATTGAATGAAACTA+TGG	0.433371	tig0041007:+15713	None:intergenic
GTTGGGCGCAGCAACATTGT+TGG	0.472629	tig0041007:+15825	None:intergenic
AGAGTTCAAGGCTCCAAGTT+GGG	0.476403	tig0041007:+15808	None:intergenic
CAGTACTATTCTCTTGCTAC+AGG	0.485288	tig0041007:-16061	Msa1460630:CDS
TTATAGGTTTGGAGCTCTTG+TGG	0.505560	tig0041007:-15886	Msa1460630:intron
CCTTGAACTCTTAACATCGT+TGG	0.541069	tig0041007:-15796	Msa1460630:CDS
AAGAGTTCAAGGCTCCAAGT+TGG	0.565418	tig0041007:+15807	None:intergenic
TACTATTCTCTTGCTACAGG+TGG	0.612717	tig0041007:-16058	Msa1460630:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATGGTTAACAAAAATTAA+AGG	+	tig0041007:15896-15915	None:intergenic	15.0%
!!!	TGTCAATATGCAATTTTTTC+AGG	+	tig0041007:15742-15761	None:intergenic	25.0%
!!	AACTCTTTGTCTTTTCATTT+AGG	+	tig0041007:15817-15836	None:intergenic	25.0%
!	AAATGAAAAGACAAAGAGTT+TGG	-	tig0041007:15817-15836	Msa1460630:CDS	25.0%
!!!	GTTTACTTTTTCTTGTTCAT+TGG	-	tig0041007:16050-16069	Msa1460630:CDS	25.0%
	GTGCAGTAAAGGAAAATAAA+TGG	+	tig0041007:15913-15932	None:intergenic	30.0%
	TTCCTTTACTGCACAATTAT+AGG	-	tig0041007:15919-15938	Msa1460630:intron	30.0%
	AACCTATAATTGTGCAGTAA+AGG	+	tig0041007:15924-15943	None:intergenic	30.0%
	TTACTGCACAATTATAGGTT+TGG	-	tig0041007:15924-15943	Msa1460630:intron	30.0%
	AGCAACATTGTTGGAATATT+GGG	+	tig0041007:15990-16009	None:intergenic	30.0%
	CAGTACATTGAATGAAACTA+TGG	+	tig0041007:16111-16130	None:intergenic	30.0%
!!!	ATGCAATTTTTTCAGGCACA+TGG	+	tig0041007:15735-15754	None:intergenic	35.0%
	CAGCAACATTGTTGGAATAT+TGG	+	tig0041007:15991-16010	None:intergenic	35.0%
!!!	TGTACCTTTTTCAAGTGAAG+TGG	-	tig0041007:16074-16093	Msa1460630:CDS	35.0%
	CAGTACTATTCTCTTGCTAC+AGG	-	tig0041007:15760-15779	Msa1460630:CDS	40.0%
	TACTATTCTCTTGCTACAGG+TGG	-	tig0041007:15763-15782	Msa1460630:CDS	40.0%
!	TTATAGGTTTGGAGCTCTTG+TGG	-	tig0041007:15935-15954	Msa1460630:intron	40.0%
	CCAACGATGTTAAGAGTTCA+AGG	+	tig0041007:16028-16047	None:intergenic	40.0%
	CCTTGAACTCTTAACATCGT+TGG	-	tig0041007:16025-16044	Msa1460630:CDS	40.0%
!	GTTGCCACTTCACTTGAAAA+AGG	+	tig0041007:16081-16100	None:intergenic	40.0%
!	TTGGCTAGAGCGTTCGTTAT+TGG	-	tig0041007:15836-15855	Msa1460630:CDS	45.0%
!	TGGCTAGAGCGTTCGTTATT+GGG	-	tig0041007:15837-15856	Msa1460630:CDS	45.0%
	AGAGTTCAAGGCTCCAAGTT+GGG	+	tig0041007:16016-16035	None:intergenic	45.0%
	AAGAGTTCAAGGCTCCAAGT+TGG	+	tig0041007:16017-16036	None:intergenic	45.0%
	GCTACAGGTGGCAATCTCTT+TGG	-	tig0041007:15775-15794	Msa1460630:CDS	50.0%
	AGCGTTCGTTATTGGGTGAG+TGG	-	tig0041007:15844-15863	Msa1460630:CDS	50.0%
!	ATGGGTCTCTGGTTCATTGC+TGG	-	tig0041007:15962-15981	Msa1460630:intron	50.0%
!	AGGTTTGGAGCTCTTGTGGC+TGG	-	tig0041007:15939-15958	Msa1460630:intron	55.0%
	TTGGAGCTCTTGTGGCTGGA+TGG	-	tig0041007:15943-15962	Msa1460630:intron	55.0%
	TGGAGCTCTTGTGGCTGGAT+GGG	-	tig0041007:15944-15963	Msa1460630:intron	55.0%
	GTTGGGCGCAGCAACATTGT+TGG	+	tig0041007:15999-16018	None:intergenic	55.0%
	CAATGTTGCTGCGCCCAACT+TGG	-	tig0041007:16000-16019	Msa1460630:CDS	55.0%
	CTTGTGGCTGGATGGGTCTC+TGG	-	tig0041007:15951-15970	Msa1460630:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
tig0041007	gene	15694	16149	15694	ID=Msa1460630;Name=Msa1460630
tig0041007	mRNA	15694	16149	15694	ID=Msa1460630-mRNA-1;Parent=Msa1460630;Name=Msa1460630-mRNA-1;_AED=0.00;_eAED=0.00;_QI=40\|1\|1\|1\|1\|1\|2\|58\|91
tig0041007	exon	15694	15902	15694	ID=Msa1460630-mRNA-1:exon:22833;Parent=Msa1460630-mRNA-1
tig0041007	exon	15985	16149	15985	ID=Msa1460630-mRNA-1:exon:22832;Parent=Msa1460630-mRNA-1
tig0041007	five_prime_UTR	16110	16149	16110	ID=Msa1460630-mRNA-1:five_prime_utr;Parent=Msa1460630-mRNA-1
tig0041007	CDS	15985	16109	15985	ID=Msa1460630-mRNA-1:cds;Parent=Msa1460630-mRNA-1
tig0041007	CDS	15752	15902	15752	ID=Msa1460630-mRNA-1:cds;Parent=Msa1460630-mRNA-1
tig0041007	three_prime_UTR	15694	15751	15694	ID=Msa1460630-mRNA-1:three_prime_utr;Parent=Msa1460630-mRNA-1

Gene Sequence	>Msa1460630 ATGTGCCTGAAAAAATTGCATATTGACAGTACTATTCTCTTGCTACAGGTGGCAATCTCTTTGGCTGCTTGCATATACTTCCTAAATGAAAAGACAAAGAGTTTGGCTAGAGCGTTCGTTATTGGGTTTGGAGCTCTTGTGGCTGGATGGGTCTCTGGTTCATTGCTGGTACCCAATATTCCAACAATGTTGCTGCGCCCAACTTGGAGCCTTGAACTCTTAACATCGTTGGTAGTTTACTTTTTCTTGTTCATTGGTTGTACCTTTTTCAAGTGA
Protein sequence	>Msa1460630 MCLKKLHIDSTILLLQVAISLAACIYFLNEKTKSLARAFVIGFGALVAGWVSGSLLVPNIPTMLLRPTWSLELLTSLVVYFFLFIGCTFFK*