AlfalfaGEDB | Msa0312650

M. sativa cultivar zhongmu-4 / Msa0312650

KEGG: K12837
GO: GO:0000243 GO:0000375 GO:0000377 GO:0000398 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005684 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008187 GO:0008380 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016604 GO:0016607 GO:0030628 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0036002 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0050896 GO:0051704 GO:0051707 GO:0070013 GO:0071004 GO:0071010 GO:0071704 GO:0089701 GO:0090304 GO:0097159 GO:0098542 GO:1901360 GO:1901363 GO:1990904
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0312650	KEH36537.1	100.000	68	0	0	2	69	292	359	1.41e-40	145

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0312650	sp\|Q9ZR40\|U2A2B_NICPL	61.194	67	26	0	2	68	506	572	4.20e-23	93.6

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0009430	Msa0312650	0.802794	4.736486e-49	-8.615850e-47
Msa0191050	Msa0312650	0.811988	5.314171e-51	-8.615850e-47
Msa0312650	Msa0439200	0.821133	4.747951e-53	-8.615850e-47
Msa0312650	Msa0493480	0.807369	5.228369e-50	-8.615850e-47
Msa0312650	Msa0493630	0.802057	6.719797e-49	-8.615850e-47
Msa0312650	Msa0521430	0.809276	2.049703e-50	-8.615850e-47
Msa0312650	Msa0548240	0.806336	8.641958e-50	-8.615850e-47
Msa0312650	Msa0607050	0.831820	1.343841e-55	-8.615850e-47
Msa0312650	Msa0647500	0.805762	1.141259e-49	-8.615850e-47
Msa0312650	Msa0649490	0.801567	8.473014e-49	-8.615850e-47
Msa0312650	Msa0690150	0.841425	4.789553e-58	-8.615850e-47
Msa0312650	Msa0690750	0.813548	2.421285e-51	-8.615850e-47
Msa0312650	Msa0709400	0.813009	3.179726e-51	-8.615850e-47
Msa0312650	Msa0849510	0.801517	8.672983e-49	-8.615850e-47
Msa0312650	Msa0968590	0.811985	5.322580e-51	-8.615850e-47
Msa0312650	Msa1012320	0.823358	1.446412e-53	-8.615850e-47
Msa0312650	Msa1124960	0.805005	1.644681e-49	-8.615850e-47
Msa0312650	Msa1170410	0.816071	6.680674e-52	-8.615850e-47
Msa0312650	Msa1209010	0.824047	9.970842e-54	-8.615850e-47
Msa0312650	Msa1227400	0.815894	7.314819e-52	-8.615850e-47
Msa0312650	Msa1239910	0.825732	3.993594e-54	-8.615850e-47
Msa0312650	Msa1254700	0.812481	4.148888e-51	-8.615850e-47
Msa0312650	Msa1267430	0.837295	5.654369e-57	-8.615850e-47
Msa0312650	Msa1294120	0.802481	5.496752e-49	-8.615850e-47
Msa0312650	Msa1294480	0.804080	2.564303e-49	-8.615850e-47
Msa0312650	Msa1357620	0.801611	8.298340e-49	-8.615850e-47
Msa0312650	Msa1361100	0.848959	4.395376e-60	-8.615850e-47
Msa0312650	Msa1460630	0.848284	6.760429e-60	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0312650	MtrunA17_Chr2g0281891	100.000	68	0	2	69	455	522	3.69e-44	147
Msa0312650	MtrunA17_Chr3g0077591	59.701	67	27	2	68	305	371	7.71e-23	89.0
Msa0312650	MtrunA17_Chr4g0013971	58.209	67	28	2	68	360	426	1.36e-21	85.9

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0312650	AT1G60830.2	58.209	67	28	2	68	44	110	1.99e-24	88.2
Msa0312650	AT1G60830.1	58.209	67	28	2	68	44	110	1.99e-24	88.2
Msa0312650	AT1G60900.1	58.209	67	28	2	68	522	588	2.81e-22	88.6
Msa0312650	AT4G36690.4	57.576	66	28	2	67	484	549	2.05e-21	85.9
Msa0312650	AT4G36690.1	57.576	66	28	2	67	506	571	2.07e-21	85.9

Find 22 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCTCTAAATGGTAGGAAATT+TGG	0.114660	2_4:-7795027	Msa0312650:CDS
CACTGGAAAATAACGTTTAT+TGG	0.182085	2_4:-7794779	Msa0312650:three_prime_UTR
CTCTAAATGGTAGGAAATTT+GGG	0.201789	2_4:-7795026	Msa0312650:CDS
GTTTCTCTTGTAGGTGTTCT+TGG	0.203987	2_4:-7795088	Msa0312650:intron
GACAGCTCTCCGTTTGGATT+TGG	0.294715	2_4:+7795374	None:intergenic
GGAGAGCTGTCTACAGGAAT+TGG	0.374888	2_4:-7795362	Msa0312650:CDS
TATGATTTGTAACCGATTGT+AGG	0.404522	2_4:-7794949	Msa0312650:exon
CCTGTAGACAGCTCTCCGTT+TGG	0.443182	2_4:+7795368	None:intergenic
CATTATATATGTAATAAACT+TGG	0.458814	2_4:+7794852	None:intergenic
ATGATTTGTAACCGATTGTA+GGG	0.489405	2_4:-7794948	Msa0312650:exon
TCTCCGTTTGGATTTGGACG+AGG	0.503529	2_4:+7795380	None:intergenic
TGATGTGTTCATGCATTCAC+TGG	0.507797	2_4:-7794796	Msa0312650:three_prime_UTR
CCAAACGGAGAGCTGTCTAC+AGG	0.512561	2_4:-7795368	Msa0312650:CDS
ATTGGAATGTACAGGGACCT+TGG	0.522185	2_4:-7795417	Msa0312650:intron
ATTATATCTCTCCCTACAAT+CGG	0.524407	2_4:+7794937	None:intergenic
CTCCGTTTGGATTTGGACGA+GGG	0.536853	2_4:+7795381	None:intergenic
ATCCCTCGTCCAAATCCAAA+CGG	0.557458	2_4:-7795383	Msa0312650:CDS
GTGAATGCATGAACACATCA+AGG	0.590146	2_4:+7794799	None:intergenic
TCTAAATGGTAGGAAATTTG+GGG	0.622587	2_4:-7795025	Msa0312650:CDS
GCTGTCTACAGGAATTGGAA+AGG	0.661551	2_4:-7795357	Msa0312650:intron
CTAAATGGTAGGAAATTTGG+GGG	0.702819	2_4:-7795024	Msa0312650:CDS
AGGGATAACAACATTCACCA+AGG	0.737659	2_4:+7795400	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CATTATATATGTAATAAACT+TGG	+	chr2_4:7795374-7795393	None:intergenic	15.0%
!!	TTAAAAGTTTCAAATCACTA+TGG	+	chr2_4:7795020-7795039	None:intergenic	20.0%
!!!	AGTGATTTGAAACTTTTAAA+TGG	-	chr2_4:7795021-7795040	Msa0312650:CDS	20.0%
!!!	GTGATTTGAAACTTTTAAAT+GGG	-	chr2_4:7795022-7795041	Msa0312650:CDS	20.0%
!!!	ATACTATGATATTTTTCTTC+TGG	+	chr2_4:7795250-7795269	None:intergenic	20.0%
!!	AGAAGAAAAATATCATAGTA+TGG	-	chr2_4:7795249-7795268	Msa0312650:intron	20.0%
!	TATAATTTCTTGCACCTTAT+TGG	-	chr2_4:7794788-7794807	Msa0312650:three_prime_UTR	25.0%
!	TAGTTGTTTAATTGTGGTTT+CGG	-	chr2_4:7795113-7795132	Msa0312650:intron	25.0%
	TCTAACAAATAAAGTATGCG+AGG	-	chr2_4:7795072-7795091	Msa0312650:CDS	30.0%
	CACAATTAAACAACTACGTT+TGG	+	chr2_4:7795109-7795128	None:intergenic	30.0%
	CAAACGTAGTTGTTTAATTG+TGG	-	chr2_4:7795107-7795126	Msa0312650:intron	30.0%
!	CAAAAAATGCTCTAAATGGT+AGG	-	chr2_4:7795188-7795207	Msa0312650:intron	30.0%
	CTCTAAATGGTAGGAAATTT+GGG	-	chr2_4:7795197-7795216	Msa0312650:intron	30.0%
	TCTAAATGGTAGGAAATTTG+GGG	-	chr2_4:7795198-7795217	Msa0312650:intron	30.0%
!	TATGATTTGTAACCGATTGT+AGG	-	chr2_4:7795274-7795293	Msa0312650:intron	30.0%
!	ATGATTTGTAACCGATTGTA+GGG	-	chr2_4:7795275-7795294	Msa0312650:intron	30.0%
	ATTATATCTCTCCCTACAAT+CGG	+	chr2_4:7795289-7795308	None:intergenic	30.0%
	AAAACTTGAGTGACAAAAAG+TGG	+	chr2_4:7795334-7795353	None:intergenic	30.0%
	CACTGGAAAATAACGTTTAT+TGG	-	chr2_4:7795444-7795463	Msa0312650:intron	30.0%
	GTTTCATACGCAAACTAAAG+TGG	+	chr2_4:7794949-7794968	None:intergenic	35.0%
!	GCTGCAAAAAATGCTCTAAA+TGG	-	chr2_4:7795184-7795203	Msa0312650:intron	35.0%
!	GCTCTAAATGGTAGGAAATT+TGG	-	chr2_4:7795196-7795215	Msa0312650:intron	35.0%
	CTAAATGGTAGGAAATTTGG+GGG	-	chr2_4:7795199-7795218	Msa0312650:intron	35.0%
	TGCACCTTATTGGAATGTAC+AGG	-	chr2_4:7794798-7794817	Msa0312650:three_prime_UTR	40.0%
	GCACCTTATTGGAATGTACA+GGG	-	chr2_4:7794799-7794818	Msa0312650:three_prime_UTR	40.0%
	AGGGATAACAACATTCACCA+AGG	+	chr2_4:7794826-7794845	None:intergenic	40.0%
	CATACAAATTTCACGCACCT+CGG	-	chr2_4:7794988-7795007	Msa0312650:CDS	40.0%
	ATACAAATTTCACGCACCTC+GGG	-	chr2_4:7794989-7795008	Msa0312650:CDS	40.0%
	TTTAAATGGGTCGAGCTTTG+TGG	-	chr2_4:7795035-7795054	Msa0312650:CDS	40.0%
	GTTTCTCTTGTAGGTGTTCT+TGG	-	chr2_4:7795135-7795154	Msa0312650:intron	40.0%
	GTGAATGCATGAACACATCA+AGG	+	chr2_4:7795427-7795446	None:intergenic	40.0%
	TGATGTGTTCATGCATTCAC+TGG	-	chr2_4:7795427-7795446	Msa0312650:intron	40.0%
	GGTCCCTGTACATTCCAATA+AGG	+	chr2_4:7794805-7794824	None:intergenic	45.0%
	ATTGGAATGTACAGGGACCT+TGG	-	chr2_4:7794806-7794825	Msa0312650:three_prime_UTR	45.0%
	ATCCCTCGTCCAAATCCAAA+CGG	-	chr2_4:7794840-7794859	Msa0312650:three_prime_UTR	45.0%
	GCTGTCTACAGGAATTGGAA+AGG	-	chr2_4:7794866-7794885	Msa0312650:three_prime_UTR	45.0%
	GTGGTTTCGGTTTCTCTTGT+AGG	-	chr2_4:7795126-7795145	Msa0312650:intron	45.0%
	CTCCGTTTGGATTTGGACGA+GGG	+	chr2_4:7794845-7794864	None:intergenic	50.0%
	TCTCCGTTTGGATTTGGACG+AGG	+	chr2_4:7794846-7794865	None:intergenic	50.0%
	GACAGCTCTCCGTTTGGATT+TGG	+	chr2_4:7794852-7794871	None:intergenic	50.0%
	GGAGAGCTGTCTACAGGAAT+TGG	-	chr2_4:7794861-7794880	Msa0312650:three_prime_UTR	50.0%
	CCTGTAGACAGCTCTCCGTT+TGG	+	chr2_4:7794858-7794877	None:intergenic	55.0%
	CCAAACGGAGAGCTGTCTAC+AGG	-	chr2_4:7794855-7794874	Msa0312650:three_prime_UTR	55.0%
	AAATTTCACGCACCTCGGGC+TGG	-	chr2_4:7794993-7795012	Msa0312650:CDS	55.0%
	AATTTCACGCACCTCGGGCT+GGG	-	chr2_4:7794994-7795013	Msa0312650:CDS	55.0%
	AATCACTATGGCCCAGCCCG+AGG	+	chr2_4:7795008-7795027	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	7794772	7795473	7794772	ID=Msa0312650;Name=Msa0312650
chr2_4	mRNA	7794772	7795473	7794772	ID=Msa0312650-mRNA-1;Parent=Msa0312650;Name=Msa0312650-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0\|0.5\|0.33\|0.66\|0.5\|0.66\|3\|188\|69
chr2_4	exon	7794772	7795097	7794772	ID=Msa0312650-mRNA-1:exon:3033;Parent=Msa0312650-mRNA-1
chr2_4	exon	7795358	7795425	7795358	ID=Msa0312650-mRNA-1:exon:3032;Parent=Msa0312650-mRNA-1
chr2_4	exon	7795470	7795473	7795470	ID=Msa0312650-mRNA-1:exon:3031;Parent=Msa0312650-mRNA-1
chr2_4	CDS	7795470	7795473	7795470	ID=Msa0312650-mRNA-1:cds;Parent=Msa0312650-mRNA-1
chr2_4	CDS	7795358	7795425	7795358	ID=Msa0312650-mRNA-1:cds;Parent=Msa0312650-mRNA-1
chr2_4	CDS	7794960	7795097	7794960	ID=Msa0312650-mRNA-1:cds;Parent=Msa0312650-mRNA-1
chr2_4	three_prime_UTR	7794772	7794959	7794772	ID=Msa0312650-mRNA-1:three_prime_utr;Parent=Msa0312650-mRNA-1

Gene Sequence	>Msa0312650 ATGGGGACCTTGGTGAATGTTGTTATCCCTCGTCCAAATCCAAACGGAGAGCTGTCTACAGGAATTGGAAAGGTGTTCTTGGAATACTCTGATTGTACTGCTTGTTTAGCTGCAAAAAATGCTCTAAATGGTAGGAAATTTGGGGGTAGTATTGTGACTGCATTTTATTATCCAGAAGAAAAATATCATAGTATGGATTATGATTTGTAA
Protein sequence	>Msa0312650 MGTLVNVVIPRPNPNGELSTGIGKVFLEYSDCTACLAAKNALNGRKFGGSIVTAFYYPEEKYHSMDYDL*