AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0064380


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0064380 MtrunA17_Chr1g0166111 100.000 77 0 0 1 77 1 77 2.79e-53 159
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0064380 AT4G14615.1 66.667 75 24 1 1 75 1 74 3.82e-32 106
Msa0064380 AT1G52825.2 68.182 66 20 1 1 66 1 65 3.26e-26 92.0
Msa0064380 AT1G52825.1 68.182 66 20 1 1 66 1 65 3.26e-26 92.0

Find 35 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCAAATCTCTCTCTTAAA+TGG 0.118498 1_2:-25423594 None:intergenic
TCTAAACTACTCTAAATAAA+AGG 0.240104 1_2:+25426168 Msa0064380:three_prime_UTR
AAGCATCAAACGAATGAAAT+TGG 0.276298 1_2:+25423565 Msa0064380:five_prime_UTR
ATTCTAGAAATTGCAAAGTT+TGG 0.311771 1_2:+25423693 Msa0064380:CDS
CGGTGGCGTATTTGTATCCT+TGG 0.314633 1_2:-25425951 None:intergenic
GAGATTTGATCTGAGTTGTT+TGG 0.379171 1_2:+25423606 Msa0064380:five_prime_UTR
CACCGCCACCAGAGGAACTT+AGG 0.405202 1_2:+25425969 Msa0064380:CDS
GAGTTCATCTTGTTTCTTCA+AGG 0.411033 1_2:+25423634 Msa0064380:five_prime_UTR
GTGGGTCGATTGTGCTTTAG+TGG 0.429882 1_2:+25426042 Msa0064380:three_prime_UTR
AATCTCTCTCTTAAATGGGC+TGG 0.433079 1_2:-25423589 None:intergenic
ATCAAATCTCTCTCTTAAAT+GGG 0.438583 1_2:-25423593 None:intergenic
ATCTCTCTCTTAAATGGGCT+GGG 0.449260 1_2:-25423588 None:intergenic
GCCATTTCCCTAAGTTCCTC+TGG 0.453339 1_2:-25425977 None:intergenic
ACCAGAGGAACTTAGGGAAA+TGG 0.455027 1_2:+25425976 Msa0064380:CDS
CTAAGTTCCTCTGGTGGCGG+TGG 0.488476 1_2:-25425968 None:intergenic
GAATTTGTGGAGGGAGTTGT+CGG 0.502242 1_2:-25423766 None:intergenic
CGAGGGAGCTAGCTCGCAAA+AGG 0.515998 1_2:+25425999 Msa0064380:CDS
CAATCACTGATGCTCCTTGT+GGG 0.517735 1_2:+25426024 Msa0064380:exon
GGTGGCGTATTTGTATCCTT+GGG 0.519575 1_2:-25425950 None:intergenic
TCCCTAAGTTCCTCTGGTGG+CGG 0.519584 1_2:-25425971 None:intergenic
AACTCCCTCCACAAATTCAT+CGG 0.541704 1_2:+25423771 Msa0064380:CDS
ACCGCCACCAGAGGAACTTA+GGG 0.541856 1_2:+25425970 Msa0064380:CDS
ATTTGATCTGAGTTGTTTGG+TGG 0.546522 1_2:+25423609 Msa0064380:five_prime_UTR
TCATCACTGGGAACATCGAA+GGG 0.547643 1_2:+25423669 Msa0064380:CDS
GTCATCACTGGGAACATCGA+AGG 0.550820 1_2:+25423668 Msa0064380:CDS
TAAACGTACATAAGAACGAT+TGG 0.555395 1_2:-25423732 None:intergenic
ACTTGAGAATTTCGCAAACA+AGG 0.566635 1_2:-25426204 None:intergenic
ACAATCACTGATGCTCCTTG+TGG 0.568209 1_2:+25426023 Msa0064380:exon
GTCATACATTGAATATCCCA+AGG 0.616788 1_2:+25425934 Msa0064380:CDS
AGGAACTTAGGGAAATGGCG+AGG 0.624927 1_2:+25425981 Msa0064380:CDS
GGAACTTAGGGAAATGGCGA+GGG 0.630297 1_2:+25425982 Msa0064380:CDS
ATTTCCCTAAGTTCCTCTGG+TGG 0.642092 1_2:-25425974 None:intergenic
AAATACGCCACCGCCACCAG+AGG 0.666326 1_2:+25425961 Msa0064380:CDS
TAAAGCACAATCGACCCACA+AGG 0.677666 1_2:-25426038 None:intergenic
CATCACTGGGAACATCGAAG+GGG 0.716260 1_2:+25423670 Msa0064380:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAATTTCTTCTATTTTATT+TGG + chr1_2:25425043-25425062 Msa0064380:intron 10.0%
!! AAGAATAAATCTAAGAAAAA+AGG - chr1_2:25423824-25423843 None:intergenic 15.0%
!!! ATTTTATTTTGATGTACTAT+CGG - chr1_2:25424382-25424401 None:intergenic 15.0%
!! AAAAAAAAAAAAACTCCTAA+TGG - chr1_2:25425724-25425743 None:intergenic 15.0%
!! TAACATAATTGCATTTATTT+GGG - chr1_2:25425854-25425873 None:intergenic 15.0%
!! TTAACATAATTGCATTTATT+TGG - chr1_2:25425855-25425874 None:intergenic 15.0%
!!! TAGTTTTTTTTTTTTTGTTG+AGG + chr1_2:25426073-25426092 Msa0064380:three_prime_UTR 15.0%
!!! AGTTTTTTTTTTTTTGTTGA+GGG + chr1_2:25426074-25426093 Msa0064380:three_prime_UTR 15.0%
!!! TTTTTCAATTAAGAAGACAA+GGG - chr1_2:25424016-25424035 None:intergenic 20.0%
!!! TTTTTTCAATTAAGAAGACA+AGG - chr1_2:25424017-25424036 None:intergenic 20.0%
!!! ATATTTATACTCTAGTTCAA+TGG + chr1_2:25424075-25424094 Msa0064380:intron 20.0%
!!! TTTTAATCTTTTGAGAAACA+GGG + chr1_2:25424454-25424473 Msa0064380:intron 20.0%
!!! GAATTAGGTATATTTTTCTT+GGG - chr1_2:25424674-25424693 None:intergenic 20.0%
!!! TGAATTAGGTATATTTTTCT+TGG - chr1_2:25424675-25424694 None:intergenic 20.0%
!!! ATTAGATTCTTCATTTTCTT+TGG + chr1_2:25424829-25424848 Msa0064380:intron 20.0%
!!! TTTTAATCTTTTGAGAAACA+GGG - chr1_2:25424454-25424473 None:intergenic 20.0%
!!! TGTAACCAATTTATTTTACA+CGG - chr1_2:25424359-25424378 None:intergenic 20.0%
!! GAAACCAAATACATTAATAA+TGG + chr1_2:25425374-25425393 Msa0064380:intron 20.0%
!! AAGTTATAACTTAATCCATT+AGG + chr1_2:25425706-25425725 Msa0064380:intron 20.0%
!! TCTAAACTACTCTAAATAAA+AGG + chr1_2:25426168-25426187 Msa0064380:three_prime_UTR 20.0%
! ATCAAATCTCTCTCTTAAAT+GGG - chr1_2:25423596-25423615 None:intergenic 25.0%
! AAAGTAAAAATGTCATCACT+GGG + chr1_2:25423657-25423676 Msa0064380:exon 25.0%
!! ATTCTAGAAATTGCAAAGTT+TGG + chr1_2:25423693-25423712 Msa0064380:CDS 25.0%
! TTAACTAAAACAGACATGTT+TGG + chr1_2:25423889-25423908 Msa0064380:intron 25.0%
! TTATCTCTATTAGTGTGATT+AGG + chr1_2:25424154-25424173 Msa0064380:intron 25.0%
!! CATCATTTTATATTTCGATC+AGG - chr1_2:25424190-25424209 None:intergenic 25.0%
! GTGTAAAATAAATTGGTTAC+AGG - chr1_2:25425077-25425096 None:intergenic 25.0%
! ACTATCGGTGTAAAATAAAT+TGG - chr1_2:25424367-25424386 None:intergenic 25.0%
! AAGATTAAAACTCAGCTTAT+AGG - chr1_2:25424444-25424463 None:intergenic 25.0%
!!! GTTTTAATCTTTTGAGAAAC+AGG + chr1_2:25424453-25424472 Msa0064380:intron 25.0%
!! CGAAGAATAAAAGCATAAAA+TGG + chr1_2:25424584-25424603 Msa0064380:intron 25.0%
! TTCAAATCGTTAATAGAATG+AGG + chr1_2:25424644-25424663 Msa0064380:intron 25.0%
! TCAAATCGTTAATAGAATGA+GGG + chr1_2:25424645-25424664 Msa0064380:intron 25.0%
!!! GTTTTAATCTTTTGAGAAAC+AGG - chr1_2:25424453-25424472 None:intergenic 25.0%
! AAGATTAAAACTCAACTTGT+AGG + chr1_2:25424995-25425014 Msa0064380:intron 25.0%
! ACTATCCGTGTAAAATAAAT+TGG + chr1_2:25425070-25425089 Msa0064380:intron 25.0%
! ATTTACATGTCACTAAAAGA+GGG + chr1_2:25425125-25425144 Msa0064380:intron 25.0%
!! CAACATTTTATATTTCGATC+AGG + chr1_2:25425257-25425276 Msa0064380:intron 25.0%
! ACCAAATACATTAATAATGG+TGG + chr1_2:25425377-25425396 Msa0064380:intron 25.0%
! TCCACCATTATTAATGTATT+TGG - chr1_2:25425381-25425400 None:intergenic 25.0%
!!! CATAATACTTTTTTGGTACT+AGG + chr1_2:25425432-25425451 Msa0064380:intron 25.0%
! TCAAATCTTGCTACATTATA+GGG - chr1_2:25425467-25425486 None:intergenic 25.0%
! TTCAAATCTTGCTACATTAT+AGG - chr1_2:25425468-25425487 None:intergenic 25.0%
! ATTGACAATACTATTGTTAC+TGG + chr1_2:25425613-25425632 Msa0064380:intron 25.0%
! GAAATCTATCTACATATTCT+TGG - chr1_2:25425651-25425670 None:intergenic 25.0%
!! TTTTTACAATAATCCAGTCT+GGG + chr1_2:25425759-25425778 Msa0064380:intron 25.0%
AAGCATCAAACGAATGAAAT+TGG + chr1_2:25423565-25423584 Msa0064380:five_prime_UTR 30.0%
GATCAAATCTCTCTCTTAAA+TGG - chr1_2:25423597-25423616 None:intergenic 30.0%
GAAAGTAAAAATGTCATCAC+TGG + chr1_2:25423656-25423675 Msa0064380:exon 30.0%
TAAACGTACATAAGAACGAT+TGG - chr1_2:25423735-25423754 None:intergenic 30.0%
!! TTACTTTTCCGATGAATTTG+TGG - chr1_2:25423782-25423801 None:intergenic 30.0%
CTGATTGAATTTGTAGAGTT+TGG - chr1_2:25424110-25424129 None:intergenic 30.0%
AGAAGAAATTTACCTTTGAG+TGG + chr1_2:25424402-25424421 Msa0064380:intron 30.0%
AAATGTGAAGAAGCTGAATT+AGG - chr1_2:25424689-25424708 None:intergenic 30.0%
TCAACAAACATACGAAGTTA+TGG - chr1_2:25424801-25424820 None:intergenic 30.0%
! AGTAGAGATCAGAATTTGTT+TGG - chr1_2:25424943-25424962 None:intergenic 30.0%
ATAGAAGAAATTTACCTGAG+TGG - chr1_2:25425037-25425056 None:intergenic 30.0%
CATTTACATGTCACTAAAAG+AGG + chr1_2:25425124-25425143 Msa0064380:intron 30.0%
! TAAAAGAGGGTAACTTTTTG+AGG + chr1_2:25425138-25425157 Msa0064380:intron 30.0%
TTACATTAGTGTGACATGTT+AGG - chr1_2:25425293-25425312 None:intergenic 30.0%
TTGTGATAGATGATCAAGAA+AGG + chr1_2:25425321-25425340 Msa0064380:intron 30.0%
!! GTATACAAGCTCTATTTTTC+CGG + chr1_2:25425403-25425422 Msa0064380:intron 30.0%
!! TATACAAGCTCTATTTTTCC+GGG + chr1_2:25425404-25425423 Msa0064380:intron 30.0%
!! AAAAAGTATTATGACAGTCC+CGG - chr1_2:25425425-25425444 None:intergenic 30.0%
!!! GGACTGTCATAATACTTTTT+TGG + chr1_2:25425425-25425444 Msa0064380:intron 30.0%
ATTATATCACTGTGAAGTAG+CGG + chr1_2:25425562-25425581 Msa0064380:intron 30.0%
TGTCAATCACAAATAACTAG+CGG - chr1_2:25425600-25425619 None:intergenic 30.0%
ACAATACTATTGTTACTGGA+GGG + chr1_2:25425617-25425636 Msa0064380:intron 30.0%
! GTTTTTACAATAATCCAGTC+TGG + chr1_2:25425758-25425777 Msa0064380:intron 30.0%
ACAAGCAATGACATTCAATT+TGG - chr1_2:25425805-25425824 None:intergenic 30.0%
AATTGAATGTCATTGCTTGT+TGG + chr1_2:25425805-25425824 Msa0064380:intron 30.0%
!!! TTTTTTTTTGTTGAGGGAAG+TGG + chr1_2:25426080-25426099 Msa0064380:three_prime_UTR 30.0%
GAGATTTGATCTGAGTTGTT+TGG + chr1_2:25423606-25423625 Msa0064380:five_prime_UTR 35.0%
ATTTGATCTGAGTTGTTTGG+TGG + chr1_2:25423609-25423628 Msa0064380:five_prime_UTR 35.0%
! GAGTTCATCTTGTTTCTTCA+AGG + chr1_2:25423634-25423653 Msa0064380:five_prime_UTR 35.0%
! TTTTCCGATGAATTTGTGGA+GGG - chr1_2:25423778-25423797 None:intergenic 35.0%
TAAGCTATAAGCTCATGAGA+AGG - chr1_2:25423924-25423943 None:intergenic 35.0%
CTCTACAAATTCAATCAGCT+AGG + chr1_2:25424112-25424131 Msa0064380:intron 35.0%
! TCAAAGAGGGTAACTTTTTG+AGG - chr1_2:25424299-25424318 None:intergenic 35.0%
ATTTCCATGTCACTCAAAGA+GGG - chr1_2:25424312-25424331 None:intergenic 35.0%
ACATGGAAATGCATGATTGA+GGG + chr1_2:25424322-25424341 Msa0064380:intron 35.0%
CATGTTCATTCGCAAACTAA+CGG - chr1_2:25424725-25424744 None:intergenic 35.0%
! TCGTATGTTTGTTGAGCATA+AGG + chr1_2:25424806-25424825 Msa0064380:intron 35.0%
AGTAATGCTCCAAATAACCA+GGG - chr1_2:25424878-25424897 None:intergenic 35.0%
TAGTAATGCTCCAAATAACC+AGG - chr1_2:25424879-25424898 None:intergenic 35.0%
TGCATGATTGAGTGATAGAA+GGG - chr1_2:25425106-25425125 None:intergenic 35.0%
ATGCATGATTGAGTGATAGA+AGG - chr1_2:25425107-25425126 None:intergenic 35.0%
GACAATACTATTGTTACTGG+AGG + chr1_2:25425616-25425635 Msa0064380:intron 35.0%
TGAGCTTCTGTTACAAGTAA+TGG - chr1_2:25425683-25425702 None:intergenic 35.0%
CAATGACATTCAATTTGGCA+AGG - chr1_2:25425800-25425819 None:intergenic 35.0%
GTCATACATTGAATATCCCA+AGG + chr1_2:25425934-25425953 Msa0064380:CDS 35.0%
ACTTGAGAATTTCGCAAACA+AGG - chr1_2:25426207-25426226 None:intergenic 35.0%
! ATCTCTCTCTTAAATGGGCT+GGG - chr1_2:25423591-25423610 None:intergenic 40.0%
AATCTCTCTCTTAAATGGGC+TGG - chr1_2:25423592-25423611 None:intergenic 40.0%
AACTCCCTCCACAAATTCAT+CGG + chr1_2:25423771-25423790 Msa0064380:CDS 40.0%
! CTTTTCCGATGAATTTGTGG+AGG - chr1_2:25423779-25423798 None:intergenic 40.0%
TAAGCTCATGAGAAGGTCAA+GGG - chr1_2:25423917-25423936 None:intergenic 40.0%
ATAAGCTCATGAGAAGGTCA+AGG - chr1_2:25423918-25423937 None:intergenic 40.0%
CATTTCCATGTCACTCAAAG+AGG - chr1_2:25424313-25424332 None:intergenic 40.0%
GACATGGAAATGCATGATTG+AGG + chr1_2:25424321-25424340 Msa0064380:intron 40.0%
ATGCATGATTGAGGGATAGA+AGG + chr1_2:25424330-25424349 Msa0064380:intron 40.0%
AACTGTTCTCTACCACTCAA+AGG - chr1_2:25424417-25424436 None:intergenic 40.0%
!! CGTTAGTTTGCGAATGAACA+TGG + chr1_2:25424723-25424742 Msa0064380:intron 40.0%
GAATGAACATGGTAGCTATG+TGG + chr1_2:25424734-25424753 Msa0064380:intron 40.0%
AGTTATGGCAAACACAGAGA+TGG - chr1_2:25424786-25424805 None:intergenic 40.0%
TGAGTGGTAGAGAACAGTTA+GGG - chr1_2:25424418-25424437 None:intergenic 40.0%
TCATCACTGGGAACATCGAA+GGG + chr1_2:25423669-25423688 Msa0064380:CDS 45.0%
GAATTTGTGGAGGGAGTTGT+CGG - chr1_2:25423769-25423788 None:intergenic 45.0%
GTTACCCTCTTTGAGTGACA+TGG + chr1_2:25424305-25424324 Msa0064380:intron 45.0%
TTAAACTTGCCTTCGTTCCC+TGG + chr1_2:25424858-25424877 Msa0064380:intron 45.0%
TCCAAATAACCAGGGAACGA+AGG - chr1_2:25424870-25424889 None:intergenic 45.0%
CTGAGTGGTAGAGAACAGTT+AGG - chr1_2:25425022-25425041 None:intergenic 45.0%
CTAACTGTTCTCTACCACTC+AGG + chr1_2:25425020-25425039 Msa0064380:intron 45.0%
GTGACGTAGCAATGCATGAT+TGG + chr1_2:25425185-25425204 Msa0064380:intron 45.0%
TTAGCTACTAAGACCCAGAC+TGG - chr1_2:25425775-25425794 None:intergenic 45.0%
GGTGGCGTATTTGTATCCTT+GGG - chr1_2:25425953-25425972 None:intergenic 45.0%
ATTTCCCTAAGTTCCTCTGG+TGG - chr1_2:25425977-25425996 None:intergenic 45.0%
ACCAGAGGAACTTAGGGAAA+TGG + chr1_2:25425976-25425995 Msa0064380:CDS 45.0%
ACAATCACTGATGCTCCTTG+TGG + chr1_2:25426023-25426042 Msa0064380:exon 45.0%
CAATCACTGATGCTCCTTGT+GGG + chr1_2:25426024-25426043 Msa0064380:exon 45.0%
TAAAGCACAATCGACCCACA+AGG - chr1_2:25426041-25426060 None:intergenic 45.0%
GTCATCACTGGGAACATCGA+AGG + chr1_2:25423668-25423687 Msa0064380:CDS 50.0%
CATCACTGGGAACATCGAAG+GGG + chr1_2:25423670-25423689 Msa0064380:CDS 50.0%
GCCTTCGTTCCCTGGTTATT+TGG + chr1_2:25424866-25424885 Msa0064380:intron 50.0%
GCTTGACTGAGACGAAAGAC+AGG - chr1_2:25425350-25425369 None:intergenic 50.0%
CGGTGGCGTATTTGTATCCT+TGG - chr1_2:25425954-25425973 None:intergenic 50.0%
GCCATTTCCCTAAGTTCCTC+TGG - chr1_2:25425980-25425999 None:intergenic 50.0%
AGGAACTTAGGGAAATGGCG+AGG + chr1_2:25425981-25426000 Msa0064380:CDS 50.0%
GGAACTTAGGGAAATGGCGA+GGG + chr1_2:25425982-25426001 Msa0064380:CDS 50.0%
GTGGGTCGATTGTGCTTTAG+TGG + chr1_2:25426042-25426061 Msa0064380:three_prime_UTR 50.0%
!! GAAACAGGGTATCGCAGCTC+AGG + chr1_2:25424468-25424487 Msa0064380:intron 55.0%
!! GAAACAGGGTATCGCAGCTC+AGG - chr1_2:25424468-25424487 None:intergenic 55.0%
ACCGCCACCAGAGGAACTTA+GGG + chr1_2:25425970-25425989 Msa0064380:CDS 55.0%
TCCCTAAGTTCCTCTGGTGG+CGG - chr1_2:25425974-25425993 None:intergenic 55.0%
AAATACGCCACCGCCACCAG+AGG + chr1_2:25425961-25425980 Msa0064380:CDS 60.0%
!! CTAAGTTCCTCTGGTGGCGG+TGG - chr1_2:25425971-25425990 None:intergenic 60.0%
CACCGCCACCAGAGGAACTT+AGG + chr1_2:25425969-25425988 Msa0064380:CDS 60.0%
CGAGGGAGCTAGCTCGCAAA+AGG + chr1_2:25425999-25426018 Msa0064380:CDS 60.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 25423557 25426267 25423557 ID=Msa0064380;Name=Msa0064380
chr1_2 mRNA 25423557 25426267 25423557 ID=Msa0064380-mRNA-1;Parent=Msa0064380;Name=Msa0064380-mRNA-1;_AED=0.01;_eAED=0.01;_QI=109|1|1|1|0|0|2|234|77
chr1_2 exon 25423557 25423797 25423557 ID=Msa0064380-mRNA-1:exon:9080;Parent=Msa0064380-mRNA-1
chr1_2 exon 25425932 25426267 25425932 ID=Msa0064380-mRNA-1:exon:9081;Parent=Msa0064380-mRNA-1
chr1_2 five_prime_UTR 25423557 25423665 25423557 ID=Msa0064380-mRNA-1:five_prime_utr;Parent=Msa0064380-mRNA-1
chr1_2 CDS 25423666 25423797 25423666 ID=Msa0064380-mRNA-1:cds;Parent=Msa0064380-mRNA-1
chr1_2 CDS 25425932 25426033 25425932 ID=Msa0064380-mRNA-1:cds;Parent=Msa0064380-mRNA-1
chr1_2 three_prime_UTR 25426034 25426267 25426034 ID=Msa0064380-mRNA-1:three_prime_utr;Parent=Msa0064380-mRNA-1
Gene Sequence

>Msa0064380

ATGTCATCACTGGGAACATCGAAGGGGATTCTAGAAATTGCAAAGTTTGGTGTATACGTAACTGTACCAATCGTTCTTATGTACGTTTATGCTAGCAATACCGACAACTCCCTCCACAAATTCATCGGAAAAAAGTCATACATTGAATATCCCAAGGATACAAATACGCCACCGCCACCAGAGGAACTTAGGGAAATGGCGAGGGAGCTAGCTCGCAAAAGGAACAATCACTGA

Protein sequence

>Msa0064380

MSSLGTSKGILEIAKFGVYVTVPIVLMYVYASNTDNSLHKFIGKKSYIEYPKDTNTPPPPEELREMARELARKRNNH*