Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50027.t1 | AES89100.2 | 97.9 | 236 | 4 | 1 | 1 | 235 | 1 | 236 | 1.10E-126 | 462.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50027.t1 | Q8GUH2 | 39.3 | 196 | 100 | 6 | 36 | 221 | 42 | 228 | 2.0e-28 | 127.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50027.t1 | G7JG61 | 97.9 | 236 | 4 | 1 | 1 | 235 | 1 | 236 | 7.8e-127 | 462.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049861 | MS.gene50027 | 0.829273 | 5.64E-55 | -1.69E-46 |
| MS.gene050271 | MS.gene50027 | 0.805585 | 1.24E-49 | -1.69E-46 |
| MS.gene051987 | MS.gene50027 | 0.838564 | 2.67E-57 | -1.69E-46 |
| MS.gene052818 | MS.gene50027 | -0.806375 | 8.48E-50 | -1.69E-46 |
| MS.gene053963 | MS.gene50027 | 0.80956 | 1.78E-50 | -1.69E-46 |
| MS.gene056849 | MS.gene50027 | 0.82372 | 1.19E-53 | -1.69E-46 |
| MS.gene056975 | MS.gene50027 | 0.818107 | 2.33E-52 | -1.69E-46 |
| MS.gene057108 | MS.gene50027 | 0.817582 | 3.06E-52 | -1.69E-46 |
| MS.gene057109 | MS.gene50027 | 0.807842 | 4.15E-50 | -1.69E-46 |
| MS.gene057110 | MS.gene50027 | 0.810935 | 9.00E-51 | -1.69E-46 |
| MS.gene057999 | MS.gene50027 | 0.819739 | 9.92E-53 | -1.69E-46 |
| MS.gene058074 | MS.gene50027 | 0.822182 | 2.72E-53 | -1.69E-46 |
| MS.gene059872 | MS.gene50027 | -0.841923 | 3.54E-58 | -1.69E-46 |
| MS.gene060297 | MS.gene50027 | 0.808102 | 3.65E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene50027 | MS.gene000647 | PPI |
| MS.gene92099 | MS.gene50027 | PPI |
| MS.gene79923 | MS.gene50027 | PPI |
| MS.gene50027 | MS.gene019686 | PPI |
| MS.gene50027 | MS.gene056832 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50027.t1 | MTR_4g070020 | 97.881 | 236 | 4 | 1 | 1 | 235 | 1 | 236 | 1.00e-171 | 473 |
| MS.gene50027.t1 | MTR_4g070020 | 97.881 | 236 | 4 | 1 | 1 | 235 | 1 | 236 | 2.22e-170 | 471 |
| MS.gene50027.t1 | MTR_1g022190 | 49.754 | 203 | 86 | 4 | 26 | 224 | 55 | 245 | 9.18e-64 | 199 |
| MS.gene50027.t1 | MTR_1g022190 | 50.246 | 203 | 85 | 4 | 26 | 224 | 30 | 220 | 3.81e-63 | 199 |
| MS.gene50027.t1 | MTR_1g022190 | 50.246 | 203 | 85 | 4 | 26 | 224 | 55 | 245 | 7.04e-63 | 199 |
| MS.gene50027.t1 | MTR_1g022190 | 50.246 | 203 | 85 | 4 | 26 | 224 | 60 | 250 | 1.05e-62 | 199 |
| MS.gene50027.t1 | MTR_8g028630 | 40.816 | 196 | 103 | 4 | 29 | 221 | 19 | 204 | 2.59e-38 | 136 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50027.t1 | AT1G19400 | 54.425 | 226 | 97 | 3 | 13 | 235 | 10 | 232 | 1.04e-80 | 242 |
| MS.gene50027.t1 | AT1G19400 | 54.425 | 226 | 97 | 3 | 13 | 235 | 10 | 232 | 6.79e-80 | 242 |
| MS.gene50027.t1 | AT1G19400 | 54.425 | 226 | 97 | 3 | 13 | 235 | 10 | 232 | 6.79e-80 | 242 |
| MS.gene50027.t1 | AT1G19400 | 54.425 | 226 | 97 | 3 | 13 | 235 | 10 | 232 | 6.79e-80 | 242 |
| MS.gene50027.t1 | AT1G75180 | 56.477 | 193 | 82 | 1 | 41 | 231 | 42 | 234 | 1.72e-74 | 226 |
| MS.gene50027.t1 | AT1G75180 | 56.477 | 193 | 82 | 1 | 41 | 231 | 42 | 234 | 1.19e-73 | 226 |
| MS.gene50027.t1 | AT1G75180 | 56.477 | 193 | 82 | 1 | 41 | 231 | 42 | 234 | 1.19e-73 | 226 |
| MS.gene50027.t1 | AT1G75180 | 56.477 | 193 | 82 | 1 | 41 | 231 | 42 | 234 | 2.25e-73 | 224 |
| MS.gene50027.t1 | AT1G01500 | 40.099 | 202 | 100 | 7 | 36 | 227 | 42 | 232 | 1.88e-36 | 131 |
Find 56 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCAGGCACAAAGTTCTTTA+AGG | 0.155540 | 4.2:+36854043 | MS.gene50027:CDS |
| GATACCCTTCTTGAAGTTAA+TGG | 0.212761 | 4.2:+36853768 | MS.gene50027:CDS |
| GAGAATGATGCAAATGAAAA+TGG | 0.217056 | 4.2:+36854218 | MS.gene50027:CDS |
| TCCTTCTGCGCTTGCTTTGC+AGG | 0.233001 | 4.2:+36854247 | MS.gene50027:CDS |
| GAGAATTCGTATAAGGTTAA+TGG | 0.283457 | 4.2:+36853567 | MS.gene50027:CDS |
| GGAAATGGACCAAGTGAAAA+TGG | 0.283915 | 4.2:+36853588 | MS.gene50027:CDS |
| ATTGCTGTTACATAATTCTA+AGG | 0.316159 | 4.2:-36853955 | None:intergenic |
| CTCATATCAACCCTCGATAA+AGG | 0.321091 | 4.2:+36853635 | MS.gene50027:CDS |
| AGAAGGGTATCAGGATTTAA+AGG | 0.331368 | 4.2:-36853756 | None:intergenic |
| GGAGAAGCAATTGTTGTTGC+CGG | 0.344534 | 4.2:+36854064 | MS.gene50027:CDS |
| AAAGATAAGCTTCTTCTGTT+TGG | 0.350138 | 4.2:+36853930 | MS.gene50027:CDS |
| TTAACTTCAAGAAGGGTATC+AGG | 0.353127 | 4.2:-36853765 | None:intergenic |
| TCGTATAAGGTTAATGGAAA+TGG | 0.363071 | 4.2:+36853573 | MS.gene50027:CDS |
| GTTAGAGAATCAAATTCTAA+TGG | 0.378979 | 4.2:+36853984 | MS.gene50027:CDS |
| TATATTGCCGGGTCCTTCTC+TGG | 0.379377 | 4.2:+36854113 | MS.gene50027:CDS |
| CGTTACCGTGGTTAGACATA+AGG | 0.381604 | 4.2:+36853661 | MS.gene50027:CDS |
| ACAATTGAGGTCTATATTGC+CGG | 0.393367 | 4.2:+36854101 | MS.gene50027:CDS |
| TTGTAAGCACTGATAGTATA+AGG | 0.394406 | 4.2:+36853874 | MS.gene50027:CDS |
| CGCGATGGAGAATTCGTATA+AGG | 0.406902 | 4.2:+36853560 | None:intergenic |
| TTCTTGAAGTTAATGGTGTT+CGG | 0.422504 | 4.2:+36853775 | MS.gene50027:CDS |
| TGATTAAGTGTGAGAATCTC+AGG | 0.428692 | 4.2:-36853729 | None:intergenic |
| CGGAGTAGTTTATATTCTGA+TGG | 0.435949 | 4.2:+36853795 | MS.gene50027:CDS |
| GCAATATAGACCTCAATTGT+TGG | 0.437528 | 4.2:-36854098 | None:intergenic |
| GGATTCCAACCCTTCTCAAA+AGG | 0.443968 | 4.2:+36853817 | MS.gene50027:CDS |
| TCCTTAAAGAACTTTGTGCC+TGG | 0.447833 | 4.2:-36854044 | None:intergenic |
| GGATAGGGTGGATAGAAAAT+CGG | 0.450168 | 4.2:+36853839 | MS.gene50027:CDS |
| TGTGAGTCGAATATAATTCC+AGG | 0.452189 | 4.2:+36854026 | MS.gene50027:CDS |
| TCATATCAACCCTCGATAAA+GGG | 0.470195 | 4.2:+36853636 | MS.gene50027:CDS |
| AGAATGATGCAAATGAAAAT+GGG | 0.470852 | 4.2:+36854219 | MS.gene50027:CDS |
| CAACCCTTCTCAAAAGGGAT+AGG | 0.480345 | 4.2:+36853823 | MS.gene50027:CDS |
| CAAATTCTAATGGACAAAGT+TGG | 0.492533 | 4.2:+36853994 | MS.gene50027:CDS |
| GTTACCGTGGTTAGACATAA+GGG | 0.505953 | 4.2:+36853662 | MS.gene50027:CDS |
| GATTCCAACCCTTCTCAAAA+GGG | 0.526688 | 4.2:+36853818 | MS.gene50027:CDS |
| GGAGTAGTTTATATTCTGAT+GGG | 0.528712 | 4.2:+36853796 | MS.gene50027:CDS |
| ACCTGCAAAGCAAGCGCAGA+AGG | 0.534939 | 4.2:-36854248 | None:intergenic |
| AACCCTTCTCAAAAGGGATA+GGG | 0.548703 | 4.2:+36853824 | MS.gene50027:CDS |
| GAGTGGTAGTGTGAAGTTTG+AGG | 0.549063 | 4.2:+36853899 | MS.gene50027:CDS |
| CTCTACTCTTCCAACAATTG+AGG | 0.556408 | 4.2:+36854088 | MS.gene50027:CDS |
| GAACACCATTAACTTCAAGA+AGG | 0.569037 | 4.2:-36853773 | None:intergenic |
| CAATTGAGGTCTATATTGCC+GGG | 0.582339 | 4.2:+36854102 | MS.gene50027:CDS |
| TCTGGAATTGCATTCAACGC+CGG | 0.588676 | 4.2:-36854197 | None:intergenic |
| AAAATGGATACTCTATTGCT+AGG | 0.589815 | 4.2:+36853604 | MS.gene50027:CDS |
| CGATAAAGGGTTCGTTACCG+TGG | 0.592561 | 4.2:+36853649 | MS.gene50027:CDS |
| AAGGTCTTCGTTAAGATGAT+CGG | 0.592566 | 4.2:-36854140 | None:intergenic |
| CGGAGTGCCAGAGAAGGACC+CGG | 0.592882 | 4.2:-36854120 | None:intergenic |
| GATGCAAATGAAAATGGGAA+AGG | 0.596141 | 4.2:+36854224 | MS.gene50027:CDS |
| AACACCATTAACTTCAAGAA+GGG | 0.596194 | 4.2:-36853772 | None:intergenic |
| CCTTCTCAAAAGGGATAGGG+TGG | 0.600087 | 4.2:+36853827 | MS.gene50027:CDS |
| ATTGTTGGAAGAGTAGAGTC+CGG | 0.603903 | 4.2:-36854083 | None:intergenic |
| CGGTAACGAACCCTTTATCG+AGG | 0.628590 | 4.2:-36853646 | None:intergenic |
| ACTGATAGTATAAGGATGAG+TGG | 0.634943 | 4.2:+36853882 | MS.gene50027:CDS |
| GATGATCGGAGTGCCAGAGA+AGG | 0.637274 | 4.2:-36854126 | None:intergenic |
| GGTAACGAACCCTTTATCGA+GGG | 0.642976 | 4.2:-36853645 | None:intergenic |
| AACACCCTTATGTCTAACCA+CGG | 0.647619 | 4.2:-36853666 | None:intergenic |
| TTAGAATTATGTAACAGCAA+TGG | 0.648523 | 4.2:+36853957 | MS.gene50027:CDS |
| AAGTGTGAGAATCTCAGGAG+TGG | 0.662685 | 4.2:-36853724 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGAATGATGCAAATGAAAAT+GGG | + | chr4.2:36854219-36854238 | MS.gene50027:CDS | 25.0% |
| ! | GTTAGAGAATCAAATTCTAA+TGG | + | chr4.2:36853984-36854003 | MS.gene50027:CDS | 25.0% |
| ! | TTAGAATTATGTAACAGCAA+TGG | + | chr4.2:36853957-36853976 | MS.gene50027:CDS | 25.0% |
| !! | ATTGCTGTTACATAATTCTA+AGG | - | chr4.2:36853958-36853977 | None:intergenic | 25.0% |
| AAAATGGATACTCTATTGCT+AGG | + | chr4.2:36853604-36853623 | MS.gene50027:CDS | 30.0% | |
| AAAGATAAGCTTCTTCTGTT+TGG | + | chr4.2:36853930-36853949 | MS.gene50027:CDS | 30.0% | |
| AACACCATTAACTTCAAGAA+GGG | - | chr4.2:36853775-36853794 | None:intergenic | 30.0% | |
| GAGAATGATGCAAATGAAAA+TGG | + | chr4.2:36854218-36854237 | MS.gene50027:CDS | 30.0% | |
| GAGAATTCGTATAAGGTTAA+TGG | + | chr4.2:36853567-36853586 | MS.gene50027:CDS | 30.0% | |
| GGAGTAGTTTATATTCTGAT+GGG | + | chr4.2:36853796-36853815 | MS.gene50027:CDS | 30.0% | |
| GTTCTCAAAAAAGACATACA+AGG | + | chr4.2:36854171-36854190 | MS.gene50027:CDS | 30.0% | |
| TCGTATAAGGTTAATGGAAA+TGG | + | chr4.2:36853573-36853592 | MS.gene50027:CDS | 30.0% | |
| TTCTTGAAGTTAATGGTGTT+CGG | + | chr4.2:36853775-36853794 | MS.gene50027:CDS | 30.0% | |
| ! | CAAATTCTAATGGACAAAGT+TGG | + | chr4.2:36853994-36854013 | MS.gene50027:CDS | 30.0% |
| ! | TTTTCATTTGCATCATTCTC+TGG | - | chr4.2:36854218-36854237 | None:intergenic | 30.0% |
| !! | ATAGAGTATCCATTTTCACT+TGG | - | chr4.2:36853600-36853619 | None:intergenic | 30.0% |
| !! | TTGTAAGCACTGATAGTATA+AGG | + | chr4.2:36853874-36853893 | MS.gene50027:CDS | 30.0% |
| !! | TTTTTGAGAACTAAGATGCA+AGG | - | chr4.2:36854162-36854181 | None:intergenic | 30.0% |
| !!! | ACATAAGGGTGTTTTATGTA+AGG | + | chr4.2:36853676-36853695 | MS.gene50027:CDS | 30.0% |
| !!! | CATAAGGGTGTTTTATGTAA+GGG | + | chr4.2:36853677-36853696 | MS.gene50027:CDS | 30.0% |
| AAAAAGACATACAAGGAAGC+CGG | + | chr4.2:36854178-36854197 | MS.gene50027:CDS | 35.0% | |
| AAGGTCTTCGTTAAGATGAT+CGG | - | chr4.2:36854143-36854162 | None:intergenic | 35.0% | |
| ACAATTGAGGTCTATATTGC+CGG | + | chr4.2:36854101-36854120 | MS.gene50027:CDS | 35.0% | |
| ACTGATAGTATAAGGATGAG+TGG | + | chr4.2:36853882-36853901 | MS.gene50027:CDS | 35.0% | |
| CGGAGTAGTTTATATTCTGA+TGG | + | chr4.2:36853795-36853814 | MS.gene50027:CDS | 35.0% | |
| GAACACCATTAACTTCAAGA+AGG | - | chr4.2:36853776-36853795 | None:intergenic | 35.0% | |
| GATACCCTTCTTGAAGTTAA+TGG | + | chr4.2:36853768-36853787 | MS.gene50027:CDS | 35.0% | |
| GATGCAAATGAAAATGGGAA+AGG | + | chr4.2:36854224-36854243 | MS.gene50027:CDS | 35.0% | |
| GCAATATAGACCTCAATTGT+TGG | - | chr4.2:36854101-36854120 | None:intergenic | 35.0% | |
| TCATATCAACCCTCGATAAA+GGG | + | chr4.2:36853636-36853655 | MS.gene50027:CDS | 35.0% | |
| TGATTAAGTGTGAGAATCTC+AGG | - | chr4.2:36853732-36853751 | None:intergenic | 35.0% | |
| TGTGAGTCGAATATAATTCC+AGG | + | chr4.2:36854026-36854045 | MS.gene50027:CDS | 35.0% | |
| !! | AGAAGGGTATCAGGATTTAA+AGG | - | chr4.2:36853759-36853778 | None:intergenic | 35.0% |
| !! | TTAACTTCAAGAAGGGTATC+AGG | - | chr4.2:36853768-36853787 | None:intergenic | 35.0% |
| AACACCCTTATGTCTAACCA+CGG | - | chr4.2:36853669-36853688 | None:intergenic | 40.0% | |
| AACCCTTCTCAAAAGGGATA+GGG | + | chr4.2:36853824-36853843 | MS.gene50027:CDS | 40.0% | |
| ATTGTTGGAAGAGTAGAGTC+CGG | - | chr4.2:36854086-36854105 | None:intergenic | 40.0% | |
| CAATTGAGGTCTATATTGCC+GGG | + | chr4.2:36854102-36854121 | MS.gene50027:CDS | 40.0% | |
| CTCATATCAACCCTCGATAA+AGG | + | chr4.2:36853635-36853654 | MS.gene50027:CDS | 40.0% | |
| CTCTACTCTTCCAACAATTG+AGG | + | chr4.2:36854088-36854107 | MS.gene50027:CDS | 40.0% | |
| GATTCCAACCCTTCTCAAAA+GGG | + | chr4.2:36853818-36853837 | MS.gene50027:CDS | 40.0% | |
| GGAAATGGACCAAGTGAAAA+TGG | + | chr4.2:36853588-36853607 | MS.gene50027:CDS | 40.0% | |
| GGATAGGGTGGATAGAAAAT+CGG | + | chr4.2:36853839-36853858 | MS.gene50027:CDS | 40.0% | |
| GTTACCGTGGTTAGACATAA+GGG | + | chr4.2:36853662-36853681 | MS.gene50027:CDS | 40.0% | |
| TCCAGGCACAAAGTTCTTTA+AGG | + | chr4.2:36854043-36854062 | MS.gene50027:CDS | 40.0% | |
| TCCTTAAAGAACTTTGTGCC+TGG | - | chr4.2:36854047-36854066 | None:intergenic | 40.0% | |
| AAGTGTGAGAATCTCAGGAG+TGG | - | chr4.2:36853727-36853746 | None:intergenic | 45.0% | |
| CAACCCTTCTCAAAAGGGAT+AGG | + | chr4.2:36853823-36853842 | MS.gene50027:CDS | 45.0% | |
| CGTTACCGTGGTTAGACATA+AGG | + | chr4.2:36853661-36853680 | MS.gene50027:CDS | 45.0% | |
| GGATTCCAACCCTTCTCAAA+AGG | + | chr4.2:36853817-36853836 | MS.gene50027:CDS | 45.0% | |
| GGTAACGAACCCTTTATCGA+GGG | - | chr4.2:36853648-36853667 | None:intergenic | 45.0% | |
| ! | GAGTGGTAGTGTGAAGTTTG+AGG | + | chr4.2:36853899-36853918 | MS.gene50027:CDS | 45.0% |
| ! | GGAGAAGCAATTGTTGTTGC+CGG | + | chr4.2:36854064-36854083 | MS.gene50027:CDS | 45.0% |
| ! | TCTGGAATTGCATTCAACGC+CGG | - | chr4.2:36854200-36854219 | None:intergenic | 45.0% |
| !! | CACCCTATCCCTTTTGAGAA+GGG | - | chr4.2:36853829-36853848 | None:intergenic | 45.0% |
| !! | CTATCCCTTTTGAGAAGGGT+TGG | - | chr4.2:36853825-36853844 | None:intergenic | 45.0% |
| CCTTCTCAAAAGGGATAGGG+TGG | + | chr4.2:36853827-36853846 | MS.gene50027:CDS | 50.0% | |
| CGATAAAGGGTTCGTTACCG+TGG | + | chr4.2:36853649-36853668 | MS.gene50027:CDS | 50.0% | |
| CGGTAACGAACCCTTTATCG+AGG | - | chr4.2:36853649-36853668 | None:intergenic | 50.0% | |
| TATATTGCCGGGTCCTTCTC+TGG | + | chr4.2:36854113-36854132 | MS.gene50027:CDS | 50.0% | |
| !! | CCACCCTATCCCTTTTGAGA+AGG | - | chr4.2:36853830-36853849 | None:intergenic | 50.0% |
| ! | GATGATCGGAGTGCCAGAGA+AGG | - | chr4.2:36854129-36854148 | None:intergenic | 55.0% |
| ! | CGGAGTGCCAGAGAAGGACC+CGG | - | chr4.2:36854123-36854142 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 36853564 | 36854268 | 36853564 | ID=MS.gene50027 |
| chr4.2 | mRNA | 36853564 | 36854268 | 36853564 | ID=MS.gene50027.t1;Parent=MS.gene50027 |
| chr4.2 | exon | 36853564 | 36854268 | 36853564 | ID=MS.gene50027.t1.exon1;Parent=MS.gene50027.t1 |
| chr4.2 | CDS | 36853564 | 36854268 | 36853564 | ID=cds.MS.gene50027.t1;Parent=MS.gene50027.t1 |
| Gene Sequence |
| Protein sequence |
