Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23256.t1 | AES89100.2 | 97.9 | 236 | 4 | 1 | 1 | 235 | 1 | 236 | 1.10E-126 | 462.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23256.t1 | Q8GUH2 | 39.3 | 196 | 100 | 6 | 36 | 221 | 42 | 228 | 2.0e-28 | 127.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23256.t1 | G7JG61 | 97.9 | 236 | 4 | 1 | 1 | 235 | 1 | 236 | 8.0e-127 | 462.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049419 | MS.gene23256 | -0.805158 | 1.53E-49 | -1.69E-46 |
| MS.gene049861 | MS.gene23256 | 0.821203 | 4.58E-53 | -1.69E-46 |
| MS.gene050271 | MS.gene23256 | 0.8031 | 4.09E-49 | -1.69E-46 |
| MS.gene051987 | MS.gene23256 | 0.81995 | 8.87E-53 | -1.69E-46 |
| MS.gene052818 | MS.gene23256 | -0.804855 | 1.77E-49 | -1.69E-46 |
| MS.gene053963 | MS.gene23256 | 0.817759 | 2.79E-52 | -1.69E-46 |
| MS.gene056849 | MS.gene23256 | 0.804017 | 2.64E-49 | -1.69E-46 |
| MS.gene056975 | MS.gene23256 | 0.803662 | 3.13E-49 | -1.69E-46 |
| MS.gene057108 | MS.gene23256 | 0.820566 | 6.41E-53 | -1.69E-46 |
| MS.gene057109 | MS.gene23256 | 0.811673 | 6.22E-51 | -1.69E-46 |
| MS.gene057110 | MS.gene23256 | 0.814291 | 1.66E-51 | -1.69E-46 |
| MS.gene057999 | MS.gene23256 | 0.819581 | 1.08E-52 | -1.69E-46 |
| MS.gene058074 | MS.gene23256 | 0.813168 | 2.93E-51 | -1.69E-46 |
| MS.gene059872 | MS.gene23256 | -0.831837 | 1.33E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene019686 | MS.gene23256 | PPI |
| MS.gene79923 | MS.gene23256 | PPI |
| MS.gene92099 | MS.gene23256 | PPI |
| MS.gene23256 | MS.gene000647 | PPI |
| MS.gene23256 | MS.gene056832 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23256.t1 | MTR_4g070020 | 97.881 | 236 | 4 | 1 | 1 | 235 | 1 | 236 | 8.57e-172 | 473 |
| MS.gene23256.t1 | MTR_4g070020 | 97.468 | 237 | 5 | 1 | 1 | 236 | 1 | 237 | 1.37e-170 | 472 |
| MS.gene23256.t1 | MTR_1g022190 | 49.754 | 203 | 86 | 4 | 26 | 224 | 55 | 245 | 7.81e-64 | 200 |
| MS.gene23256.t1 | MTR_1g022190 | 50.246 | 203 | 85 | 4 | 26 | 224 | 30 | 220 | 3.43e-63 | 200 |
| MS.gene23256.t1 | MTR_1g022190 | 50.246 | 203 | 85 | 4 | 26 | 224 | 55 | 245 | 6.84e-63 | 200 |
| MS.gene23256.t1 | MTR_1g022190 | 50.246 | 203 | 85 | 4 | 26 | 224 | 60 | 250 | 1.22e-62 | 199 |
| MS.gene23256.t1 | MTR_8g028630 | 40.816 | 196 | 103 | 4 | 29 | 221 | 19 | 204 | 3.28e-38 | 136 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23256.t1 | AT1G19400 | 54.626 | 227 | 97 | 3 | 13 | 236 | 10 | 233 | 1.52e-81 | 244 |
| MS.gene23256.t1 | AT1G19400 | 53.712 | 229 | 100 | 3 | 13 | 238 | 10 | 235 | 6.43e-80 | 242 |
| MS.gene23256.t1 | AT1G19400 | 53.712 | 229 | 100 | 3 | 13 | 238 | 10 | 235 | 6.43e-80 | 242 |
| MS.gene23256.t1 | AT1G19400 | 53.712 | 229 | 100 | 3 | 13 | 238 | 10 | 235 | 6.43e-80 | 242 |
| MS.gene23256.t1 | AT1G75180 | 56.477 | 193 | 82 | 1 | 41 | 231 | 42 | 234 | 2.35e-74 | 226 |
| MS.gene23256.t1 | AT1G75180 | 56.281 | 199 | 84 | 2 | 41 | 236 | 42 | 240 | 1.08e-73 | 227 |
| MS.gene23256.t1 | AT1G75180 | 56.281 | 199 | 84 | 2 | 41 | 236 | 42 | 240 | 1.08e-73 | 227 |
| MS.gene23256.t1 | AT1G75180 | 56.281 | 199 | 84 | 2 | 41 | 236 | 42 | 240 | 2.50e-73 | 224 |
| MS.gene23256.t1 | AT1G01500 | 40.099 | 202 | 100 | 7 | 36 | 227 | 42 | 232 | 2.21e-36 | 131 |
Find 56 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCAGGCACAAAGTTCTTTA+AGG | 0.155540 | 4.2:+36901269 | MS.gene23256:CDS |
| GATACCCTTCTTGAAGTTAA+TGG | 0.212761 | 4.2:+36900994 | MS.gene23256:CDS |
| GAGAATGATGCAAATGAAAA+TGG | 0.217056 | 4.2:+36901444 | MS.gene23256:CDS |
| TCCTTCTGCGCTTGCTTTGC+AGG | 0.233001 | 4.2:+36901473 | MS.gene23256:CDS |
| GAGAATTCGTATAAGGTTAA+TGG | 0.283457 | 4.2:+36900793 | MS.gene23256:CDS |
| GGAAATGGACCAAGTGAAAA+TGG | 0.283915 | 4.2:+36900814 | MS.gene23256:CDS |
| ATTGCTGTTACATAATTCTA+AGG | 0.316159 | 4.2:-36901181 | None:intergenic |
| CTCATATCAACCCTCGATAA+AGG | 0.321091 | 4.2:+36900861 | MS.gene23256:CDS |
| AGAAGGGTATCAGGATTTAA+AGG | 0.331368 | 4.2:-36900982 | None:intergenic |
| GGAGAAGCAATTGTTGTTGC+CGG | 0.344534 | 4.2:+36901290 | MS.gene23256:CDS |
| AAAGATAAGCTTCTTCTGTT+TGG | 0.350138 | 4.2:+36901156 | MS.gene23256:CDS |
| TTAACTTCAAGAAGGGTATC+AGG | 0.353127 | 4.2:-36900991 | None:intergenic |
| TCGTATAAGGTTAATGGAAA+TGG | 0.363071 | 4.2:+36900799 | MS.gene23256:CDS |
| GTTAGAGAATCAAATTCTAA+TGG | 0.378979 | 4.2:+36901210 | MS.gene23256:CDS |
| TATATTGCCGGGTCCTTCTC+TGG | 0.379377 | 4.2:+36901339 | MS.gene23256:CDS |
| CGTTACCGTGGTTAGACATA+AGG | 0.381604 | 4.2:+36900887 | MS.gene23256:CDS |
| ACAATTGAGGTCTATATTGC+CGG | 0.393367 | 4.2:+36901327 | MS.gene23256:CDS |
| TTGTAAGCACTGATAGTATA+AGG | 0.394406 | 4.2:+36901100 | MS.gene23256:CDS |
| CGCGATGGAGAATTCGTATA+AGG | 0.406902 | 4.2:+36900786 | None:intergenic |
| TTCTTGAAGTTAATGGTGTT+CGG | 0.422504 | 4.2:+36901001 | MS.gene23256:CDS |
| TGATTAAGTGTGAGAATCTC+AGG | 0.428692 | 4.2:-36900955 | None:intergenic |
| CGGAGTAGTTTATATTCTGA+TGG | 0.435949 | 4.2:+36901021 | MS.gene23256:CDS |
| GCAATATAGACCTCAATTGT+TGG | 0.437528 | 4.2:-36901324 | None:intergenic |
| GGATTCCAACCCTTCTCAAA+AGG | 0.443968 | 4.2:+36901043 | MS.gene23256:CDS |
| TCCTTAAAGAACTTTGTGCC+TGG | 0.447833 | 4.2:-36901270 | None:intergenic |
| GGATAGGGTGGATAGAAAAT+CGG | 0.450168 | 4.2:+36901065 | MS.gene23256:CDS |
| TGTGAGTCGAATATAATTCC+AGG | 0.452189 | 4.2:+36901252 | MS.gene23256:CDS |
| TCATATCAACCCTCGATAAA+GGG | 0.470195 | 4.2:+36900862 | MS.gene23256:CDS |
| AGAATGATGCAAATGAAAAT+GGG | 0.470852 | 4.2:+36901445 | MS.gene23256:CDS |
| CAACCCTTCTCAAAAGGGAT+AGG | 0.480345 | 4.2:+36901049 | MS.gene23256:CDS |
| CAAATTCTAATGGACAAAGT+TGG | 0.492533 | 4.2:+36901220 | MS.gene23256:CDS |
| GTTACCGTGGTTAGACATAA+GGG | 0.505953 | 4.2:+36900888 | MS.gene23256:CDS |
| GATTCCAACCCTTCTCAAAA+GGG | 0.526688 | 4.2:+36901044 | MS.gene23256:CDS |
| GGAGTAGTTTATATTCTGAT+GGG | 0.528712 | 4.2:+36901022 | MS.gene23256:CDS |
| ACCTGCAAAGCAAGCGCAGA+AGG | 0.534939 | 4.2:-36901474 | None:intergenic |
| AACCCTTCTCAAAAGGGATA+GGG | 0.548703 | 4.2:+36901050 | MS.gene23256:CDS |
| GAGTGGTAGTGTGAAGTTTG+AGG | 0.549063 | 4.2:+36901125 | MS.gene23256:CDS |
| CTCTACTCTTCCAACAATTG+AGG | 0.556408 | 4.2:+36901314 | MS.gene23256:CDS |
| GAACACCATTAACTTCAAGA+AGG | 0.569037 | 4.2:-36900999 | None:intergenic |
| CAATTGAGGTCTATATTGCC+GGG | 0.582339 | 4.2:+36901328 | MS.gene23256:CDS |
| TCTGGAATTGCATTCAACGC+CGG | 0.588676 | 4.2:-36901423 | None:intergenic |
| AAAATGGATACTCTATTGCT+AGG | 0.589815 | 4.2:+36900830 | MS.gene23256:CDS |
| CGATAAAGGGTTCGTTACCG+TGG | 0.592561 | 4.2:+36900875 | MS.gene23256:CDS |
| AAGGTCTTCGTTAAGATGAT+CGG | 0.592566 | 4.2:-36901366 | None:intergenic |
| CGGAGTGCCAGAGAAGGACC+CGG | 0.592882 | 4.2:-36901346 | None:intergenic |
| GATGCAAATGAAAATGGGAA+AGG | 0.596141 | 4.2:+36901450 | MS.gene23256:CDS |
| AACACCATTAACTTCAAGAA+GGG | 0.596194 | 4.2:-36900998 | None:intergenic |
| CCTTCTCAAAAGGGATAGGG+TGG | 0.600087 | 4.2:+36901053 | MS.gene23256:CDS |
| ATTGTTGGAAGAGTAGAGTC+CGG | 0.603903 | 4.2:-36901309 | None:intergenic |
| CGGTAACGAACCCTTTATCG+AGG | 0.628590 | 4.2:-36900872 | None:intergenic |
| ACTGATAGTATAAGGATGAG+TGG | 0.634943 | 4.2:+36901108 | MS.gene23256:CDS |
| GATGATCGGAGTGCCAGAGA+AGG | 0.637274 | 4.2:-36901352 | None:intergenic |
| GGTAACGAACCCTTTATCGA+GGG | 0.642976 | 4.2:-36900871 | None:intergenic |
| AACACCCTTATGTCTAACCA+CGG | 0.647619 | 4.2:-36900892 | None:intergenic |
| TTAGAATTATGTAACAGCAA+TGG | 0.648523 | 4.2:+36901183 | MS.gene23256:CDS |
| AAGTGTGAGAATCTCAGGAG+TGG | 0.662685 | 4.2:-36900950 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGAATGATGCAAATGAAAAT+GGG | + | chr4.2:36901445-36901464 | MS.gene23256:CDS | 25.0% |
| ! | GTTAGAGAATCAAATTCTAA+TGG | + | chr4.2:36901210-36901229 | MS.gene23256:CDS | 25.0% |
| ! | TTAGAATTATGTAACAGCAA+TGG | + | chr4.2:36901183-36901202 | MS.gene23256:CDS | 25.0% |
| !! | ATTGCTGTTACATAATTCTA+AGG | - | chr4.2:36901184-36901203 | None:intergenic | 25.0% |
| AAAATGGATACTCTATTGCT+AGG | + | chr4.2:36900830-36900849 | MS.gene23256:CDS | 30.0% | |
| AAAGATAAGCTTCTTCTGTT+TGG | + | chr4.2:36901156-36901175 | MS.gene23256:CDS | 30.0% | |
| AACACCATTAACTTCAAGAA+GGG | - | chr4.2:36901001-36901020 | None:intergenic | 30.0% | |
| GAGAATGATGCAAATGAAAA+TGG | + | chr4.2:36901444-36901463 | MS.gene23256:CDS | 30.0% | |
| GAGAATTCGTATAAGGTTAA+TGG | + | chr4.2:36900793-36900812 | MS.gene23256:CDS | 30.0% | |
| GGAGTAGTTTATATTCTGAT+GGG | + | chr4.2:36901022-36901041 | MS.gene23256:CDS | 30.0% | |
| GTTCTCAAAAAAGACATACA+AGG | + | chr4.2:36901397-36901416 | MS.gene23256:CDS | 30.0% | |
| TCGTATAAGGTTAATGGAAA+TGG | + | chr4.2:36900799-36900818 | MS.gene23256:CDS | 30.0% | |
| TTCTTGAAGTTAATGGTGTT+CGG | + | chr4.2:36901001-36901020 | MS.gene23256:CDS | 30.0% | |
| ! | CAAATTCTAATGGACAAAGT+TGG | + | chr4.2:36901220-36901239 | MS.gene23256:CDS | 30.0% |
| ! | TTTTCATTTGCATCATTCTC+TGG | - | chr4.2:36901444-36901463 | None:intergenic | 30.0% |
| !! | ATAGAGTATCCATTTTCACT+TGG | - | chr4.2:36900826-36900845 | None:intergenic | 30.0% |
| !! | TTGTAAGCACTGATAGTATA+AGG | + | chr4.2:36901100-36901119 | MS.gene23256:CDS | 30.0% |
| !! | TTTTTGAGAACTAAGATGCA+AGG | - | chr4.2:36901388-36901407 | None:intergenic | 30.0% |
| !!! | ACATAAGGGTGTTTTATGTA+AGG | + | chr4.2:36900902-36900921 | MS.gene23256:CDS | 30.0% |
| !!! | CATAAGGGTGTTTTATGTAA+GGG | + | chr4.2:36900903-36900922 | MS.gene23256:CDS | 30.0% |
| AAAAAGACATACAAGGAAGC+CGG | + | chr4.2:36901404-36901423 | MS.gene23256:CDS | 35.0% | |
| AAGGTCTTCGTTAAGATGAT+CGG | - | chr4.2:36901369-36901388 | None:intergenic | 35.0% | |
| ACAATTGAGGTCTATATTGC+CGG | + | chr4.2:36901327-36901346 | MS.gene23256:CDS | 35.0% | |
| ACTGATAGTATAAGGATGAG+TGG | + | chr4.2:36901108-36901127 | MS.gene23256:CDS | 35.0% | |
| CGGAGTAGTTTATATTCTGA+TGG | + | chr4.2:36901021-36901040 | MS.gene23256:CDS | 35.0% | |
| GAACACCATTAACTTCAAGA+AGG | - | chr4.2:36901002-36901021 | None:intergenic | 35.0% | |
| GATACCCTTCTTGAAGTTAA+TGG | + | chr4.2:36900994-36901013 | MS.gene23256:CDS | 35.0% | |
| GATGCAAATGAAAATGGGAA+AGG | + | chr4.2:36901450-36901469 | MS.gene23256:CDS | 35.0% | |
| GCAATATAGACCTCAATTGT+TGG | - | chr4.2:36901327-36901346 | None:intergenic | 35.0% | |
| TCATATCAACCCTCGATAAA+GGG | + | chr4.2:36900862-36900881 | MS.gene23256:CDS | 35.0% | |
| TGATTAAGTGTGAGAATCTC+AGG | - | chr4.2:36900958-36900977 | None:intergenic | 35.0% | |
| TGTGAGTCGAATATAATTCC+AGG | + | chr4.2:36901252-36901271 | MS.gene23256:CDS | 35.0% | |
| !! | AGAAGGGTATCAGGATTTAA+AGG | - | chr4.2:36900985-36901004 | None:intergenic | 35.0% |
| !! | TTAACTTCAAGAAGGGTATC+AGG | - | chr4.2:36900994-36901013 | None:intergenic | 35.0% |
| AACACCCTTATGTCTAACCA+CGG | - | chr4.2:36900895-36900914 | None:intergenic | 40.0% | |
| AACCCTTCTCAAAAGGGATA+GGG | + | chr4.2:36901050-36901069 | MS.gene23256:CDS | 40.0% | |
| ATTGTTGGAAGAGTAGAGTC+CGG | - | chr4.2:36901312-36901331 | None:intergenic | 40.0% | |
| CAATTGAGGTCTATATTGCC+GGG | + | chr4.2:36901328-36901347 | MS.gene23256:CDS | 40.0% | |
| CTCATATCAACCCTCGATAA+AGG | + | chr4.2:36900861-36900880 | MS.gene23256:CDS | 40.0% | |
| CTCTACTCTTCCAACAATTG+AGG | + | chr4.2:36901314-36901333 | MS.gene23256:CDS | 40.0% | |
| GATTCCAACCCTTCTCAAAA+GGG | + | chr4.2:36901044-36901063 | MS.gene23256:CDS | 40.0% | |
| GGAAATGGACCAAGTGAAAA+TGG | + | chr4.2:36900814-36900833 | MS.gene23256:CDS | 40.0% | |
| GGATAGGGTGGATAGAAAAT+CGG | + | chr4.2:36901065-36901084 | MS.gene23256:CDS | 40.0% | |
| GTTACCGTGGTTAGACATAA+GGG | + | chr4.2:36900888-36900907 | MS.gene23256:CDS | 40.0% | |
| TCCAGGCACAAAGTTCTTTA+AGG | + | chr4.2:36901269-36901288 | MS.gene23256:CDS | 40.0% | |
| TCCTTAAAGAACTTTGTGCC+TGG | - | chr4.2:36901273-36901292 | None:intergenic | 40.0% | |
| AAGTGTGAGAATCTCAGGAG+TGG | - | chr4.2:36900953-36900972 | None:intergenic | 45.0% | |
| CAACCCTTCTCAAAAGGGAT+AGG | + | chr4.2:36901049-36901068 | MS.gene23256:CDS | 45.0% | |
| CGTTACCGTGGTTAGACATA+AGG | + | chr4.2:36900887-36900906 | MS.gene23256:CDS | 45.0% | |
| GGATTCCAACCCTTCTCAAA+AGG | + | chr4.2:36901043-36901062 | MS.gene23256:CDS | 45.0% | |
| GGTAACGAACCCTTTATCGA+GGG | - | chr4.2:36900874-36900893 | None:intergenic | 45.0% | |
| ! | GAGTGGTAGTGTGAAGTTTG+AGG | + | chr4.2:36901125-36901144 | MS.gene23256:CDS | 45.0% |
| ! | GGAGAAGCAATTGTTGTTGC+CGG | + | chr4.2:36901290-36901309 | MS.gene23256:CDS | 45.0% |
| ! | TCTGGAATTGCATTCAACGC+CGG | - | chr4.2:36901426-36901445 | None:intergenic | 45.0% |
| !! | CACCCTATCCCTTTTGAGAA+GGG | - | chr4.2:36901055-36901074 | None:intergenic | 45.0% |
| !! | CTATCCCTTTTGAGAAGGGT+TGG | - | chr4.2:36901051-36901070 | None:intergenic | 45.0% |
| CCTTCTCAAAAGGGATAGGG+TGG | + | chr4.2:36901053-36901072 | MS.gene23256:CDS | 50.0% | |
| CGATAAAGGGTTCGTTACCG+TGG | + | chr4.2:36900875-36900894 | MS.gene23256:CDS | 50.0% | |
| CGGTAACGAACCCTTTATCG+AGG | - | chr4.2:36900875-36900894 | None:intergenic | 50.0% | |
| TATATTGCCGGGTCCTTCTC+TGG | + | chr4.2:36901339-36901358 | MS.gene23256:CDS | 50.0% | |
| !! | CCACCCTATCCCTTTTGAGA+AGG | - | chr4.2:36901056-36901075 | None:intergenic | 50.0% |
| ACCTGCAAAGCAAGCGCAGA+AGG | - | chr4.2:36901477-36901496 | None:intergenic | 55.0% | |
| TCCTTCTGCGCTTGCTTTGC+AGG | + | chr4.2:36901473-36901492 | MS.gene23256:CDS | 55.0% | |
| ! | GATGATCGGAGTGCCAGAGA+AGG | - | chr4.2:36901355-36901374 | None:intergenic | 55.0% |
| ! | CGGAGTGCCAGAGAAGGACC+CGG | - | chr4.2:36901349-36901368 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 36900790 | 36901512 | 36900790 | ID=MS.gene23256 |
| chr4.2 | mRNA | 36900790 | 36901512 | 36900790 | ID=MS.gene23256.t1;Parent=MS.gene23256 |
| chr4.2 | exon | 36900790 | 36901512 | 36900790 | ID=MS.gene23256.t1.exon1;Parent=MS.gene23256.t1 |
| chr4.2 | CDS | 36900790 | 36901512 | 36900790 | ID=cds.MS.gene23256.t1;Parent=MS.gene23256.t1 |
| Gene Sequence |
| Protein sequence |
