Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001404.t1 | RHN78238.1 | 96.3 | 348 | 13 | 0 | 1 | 348 | 103 | 450 | 5.70E-204 | 719.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001404.t1 | Q9CA34 | 64.2 | 352 | 119 | 3 | 1 | 350 | 99 | 445 | 4.7e-138 | 492.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001404.t1 | A0A396JPN5 | 96.3 | 348 | 13 | 0 | 1 | 348 | 103 | 450 | 4.1e-204 | 719.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene045350 | MS.gene001404 | PPI |
| MS.gene035337 | MS.gene001404 | PPI |
| MS.gene58506 | MS.gene001404 | PPI |
| MS.gene001404 | MS.gene041562 | PPI |
| MS.gene001404 | MS.gene75663 | PPI |
| MS.gene001404 | MS.gene77538 | PPI |
| MS.gene001404 | MS.gene036481 | PPI |
| MS.gene79900 | MS.gene001404 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001404.t1 | MTR_1g035730 | 95.690 | 348 | 15 | 0 | 1 | 348 | 103 | 450 | 0.0 | 707 |
| MS.gene001404.t1 | MTR_1g069460 | 46.197 | 355 | 182 | 3 | 1 | 348 | 185 | 537 | 7.01e-107 | 326 |
| MS.gene001404.t1 | MTR_7g098650 | 45.352 | 355 | 185 | 3 | 1 | 348 | 110 | 462 | 2.81e-105 | 320 |
| MS.gene001404.t1 | MTR_5g055350 | 43.094 | 362 | 186 | 5 | 1 | 348 | 83 | 438 | 2.15e-96 | 294 |
| MS.gene001404.t1 | MTR_3g084400 | 42.162 | 370 | 199 | 4 | 1 | 358 | 98 | 464 | 1.18e-95 | 293 |
| MS.gene001404.t1 | MTR_4g129930 | 42.618 | 359 | 192 | 4 | 1 | 348 | 102 | 457 | 2.10e-95 | 292 |
| MS.gene001404.t1 | MTR_5g095940 | 42.222 | 360 | 195 | 3 | 1 | 348 | 125 | 483 | 2.16e-90 | 280 |
| MS.gene001404.t1 | MTR_5g010370 | 40.113 | 354 | 200 | 9 | 1 | 348 | 191 | 538 | 2.94e-86 | 271 |
| MS.gene001404.t1 | MTR_3g007840 | 40.000 | 360 | 180 | 4 | 1 | 348 | 53 | 388 | 1.24e-83 | 260 |
| MS.gene001404.t1 | MTR_1g102430 | 40.997 | 361 | 192 | 10 | 2 | 348 | 132 | 485 | 1.05e-82 | 260 |
| MS.gene001404.t1 | MTR_5g010350 | 39.266 | 354 | 203 | 9 | 1 | 348 | 167 | 514 | 1.10e-81 | 258 |
| MS.gene001404.t1 | MTR_4g119810 | 35.310 | 371 | 212 | 11 | 1 | 347 | 73 | 439 | 2.98e-64 | 211 |
| MS.gene001404.t1 | MTR_5g095950 | 34.444 | 360 | 192 | 4 | 1 | 348 | 98 | 425 | 1.65e-63 | 210 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001404.t1 | AT1G68470 | 64.205 | 352 | 119 | 3 | 1 | 350 | 99 | 445 | 2.63e-173 | 490 |
| MS.gene001404.t1 | AT2G20370 | 45.352 | 355 | 185 | 3 | 1 | 348 | 182 | 534 | 6.39e-103 | 316 |
| MS.gene001404.t1 | AT2G29040 | 45.787 | 356 | 183 | 6 | 1 | 348 | 330 | 683 | 3.54e-102 | 317 |
| MS.gene001404.t1 | AT4G13990 | 43.697 | 357 | 188 | 6 | 1 | 348 | 128 | 480 | 9.25e-97 | 297 |
| MS.gene001404.t1 | AT4G13990 | 43.697 | 357 | 188 | 6 | 1 | 348 | 128 | 480 | 9.25e-97 | 297 |
| MS.gene001404.t1 | AT2G31990 | 41.713 | 362 | 194 | 9 | 1 | 348 | 106 | 464 | 6.87e-91 | 281 |
| MS.gene001404.t1 | AT2G31990 | 41.713 | 362 | 194 | 9 | 1 | 348 | 114 | 472 | 8.40e-91 | 281 |
| MS.gene001404.t1 | AT2G31990 | 41.713 | 362 | 194 | 9 | 1 | 348 | 152 | 510 | 2.60e-90 | 281 |
| MS.gene001404.t1 | AT5G62220 | 42.276 | 369 | 190 | 12 | 2 | 355 | 150 | 510 | 6.08e-90 | 280 |
| MS.gene001404.t1 | AT2G32750 | 40.000 | 360 | 195 | 8 | 1 | 345 | 114 | 467 | 9.67e-87 | 271 |
| MS.gene001404.t1 | AT2G32750 | 40.000 | 360 | 195 | 8 | 1 | 345 | 116 | 469 | 1.04e-86 | 271 |
| MS.gene001404.t1 | AT2G32740 | 38.798 | 366 | 198 | 9 | 2 | 348 | 98 | 456 | 1.45e-85 | 266 |
| MS.gene001404.t1 | AT5G41250 | 38.418 | 354 | 203 | 8 | 1 | 348 | 208 | 552 | 1.93e-82 | 261 |
| MS.gene001404.t1 | AT1G63450 | 39.093 | 353 | 204 | 9 | 1 | 348 | 310 | 656 | 1.61e-79 | 256 |
| MS.gene001404.t1 | AT4G22580 | 36.043 | 369 | 206 | 13 | 1 | 348 | 68 | 427 | 3.45e-65 | 213 |
| MS.gene001404.t1 | AT1G67410 | 26.515 | 264 | 134 | 10 | 25 | 264 | 103 | 330 | 2.41e-12 | 68.2 |
Find 0 sgRNAs with CRISPR-Local
Find 114 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CCAATAAATGAAAACAAAAA+CGG | - | 11231:5137-5156 | MS.gene001404:intergenic | 20.0% |
| !!! | AAAGATCATTTTATTTCTCT+GGG | + | 11231:4891-4910 | MS.gene001404:CDS | 20.0% |
| !!! | TAAAGATCATTTTATTTCTC+TGG | + | 11231:4890-4909 | MS.gene001404:CDS | 20.0% |
| !!! | TTTTGTTTTCATTTATTGGT+GGG | + | 11231:5138-5157 | MS.gene001404:CDS | 20.0% |
| !!! | TTTTTGTTTTCATTTATTGG+TGG | + | 11231:5137-5156 | MS.gene001404:CDS | 20.0% |
| ! | AAAGAAGAGTAATATGATTG+AGG | + | 11231:5484-5503 | MS.gene001404:CDS | 25.0% |
| ! | AAATCATTTCTGCTAAGAAT+TGG | - | 11231:4677-4696 | MS.gene001404:intergenic | 25.0% |
| ! | TAAGGAGAAAGATACTTATT+CGG | + | 11231:5436-5455 | MS.gene001404:CDS | 25.0% |
| ! | TAGAATAGAATTGCTTGATT+TGG | - | 11231:4741-4760 | MS.gene001404:intergenic | 25.0% |
| ! | TATTCGGTTTATATTGAAGA+GGG | + | 11231:5452-5471 | MS.gene001404:CDS | 25.0% |
| ! | TGGTAGGTTTAATAATATGT+TGG | - | 11231:4967-4986 | MS.gene001404:intergenic | 25.0% |
| ! | TTATTCGGTTTATATTGAAG+AGG | + | 11231:5451-5470 | MS.gene001404:CDS | 25.0% |
| !!! | AGCATTTCTTCTTTTGTTTT+TGG | - | 11231:5080-5099 | MS.gene001404:intergenic | 25.0% |
| !!! | CCGTTTTTGTTTTCATTTAT+TGG | + | 11231:5134-5153 | MS.gene001404:CDS | 25.0% |
| ATAAAATGAGACAAAACGAC+AGG | + | 11231:5111-5130 | MS.gene001404:CDS | 30.0% | |
| ATTCGGTTTATATTGAAGAG+GGG | + | 11231:5453-5472 | MS.gene001404:CDS | 30.0% | |
| CTATTCTACATACCATTCTA+CGG | + | 11231:4753-4772 | MS.gene001404:CDS | 30.0% | |
| GAATAAGTATCTTTCTCCTT+AGG | - | 11231:5437-5456 | MS.gene001404:intergenic | 30.0% | |
| TACATGATCGATTAGATCAA+CGG | - | 11231:4838-4857 | MS.gene001404:intergenic | 30.0% | |
| TGGGAAGGTTATAATCAAAA+CGG | + | 11231:5029-5048 | MS.gene001404:CDS | 30.0% | |
| TTATTGGTGGGAAAAGAAAA+GGG | + | 11231:5150-5169 | MS.gene001404:CDS | 30.0% | |
| TTTATTGGTGGGAAAAGAAA+AGG | + | 11231:5149-5168 | MS.gene001404:CDS | 30.0% | |
| ! | TTTTTGGATGAAAATACGAC+GGG | - | 11231:5064-5083 | MS.gene001404:intergenic | 30.0% |
| !! | ATCATTTTATTTCTCTGGGA+AGG | + | 11231:4895-4914 | MS.gene001404:CDS | 30.0% |
| !! | GTTTTTGGATGAAAATACGA+CGG | - | 11231:5065-5084 | MS.gene001404:intergenic | 30.0% |
| !! | TAATATGTTGGCTCCAAAAT+CGG | - | 11231:4955-4974 | MS.gene001404:intergenic | 30.0% |
| !! | TTTTGATTATAACCTTCCCA+AGG | - | 11231:5029-5048 | MS.gene001404:intergenic | 30.0% |
| AAGCCATTGTTCTTCACATA+AGG | - | 11231:4615-4634 | MS.gene001404:intergenic | 35.0% | |
| AGCCATTGTTCTTCACATAA+GGG | - | 11231:4614-4633 | MS.gene001404:intergenic | 35.0% | |
| ATAACACTAAGCACTTCCAT+TGG | - | 11231:5278-5297 | MS.gene001404:intergenic | 35.0% | |
| ATACCATTGGTATTTGCCTA+AGG | + | 11231:5418-5437 | MS.gene001404:CDS | 35.0% | |
| ATCATGTACAAAGTCTACCT+TGG | + | 11231:4850-4869 | MS.gene001404:CDS | 35.0% | |
| CTTCTAGTAAAAGAATCACC+TGG | - | 11231:5329-5348 | MS.gene001404:intergenic | 35.0% | |
| GAAGAGTAATATGATTGAGG+AGG | + | 11231:5487-5506 | MS.gene001404:CDS | 35.0% | |
| GTATGTGGAGAGAAAAATAC+AGG | - | 11231:5386-5405 | MS.gene001404:intergenic | 35.0% | |
| TAGTAAATGCCATCAACCAA+TGG | + | 11231:5259-5278 | MS.gene001404:CDS | 35.0% | |
| TATGTGAAGAACAATGGCTT+AGG | + | 11231:4615-4634 | MS.gene001404:CDS | 35.0% | |
| TTCGGTTTATATTGAAGAGG+GGG | + | 11231:5454-5473 | MS.gene001404:CDS | 35.0% | |
| TTGCACTTCAAGGAATTTCT+AGG | + | 11231:5633-5652 | MS.gene001404:CDS | 35.0% | |
| ! | CAGAAATGATTTTCCATGCT+AGG | + | 11231:4685-4704 | MS.gene001404:CDS | 35.0% |
| ! | CAGGTGATTCTTTTACTAGA+AGG | + | 11231:5327-5346 | MS.gene001404:CDS | 35.0% |
| !! | AATTTCTAGGCTTTTGTTCG+AGG | + | 11231:5646-5665 | MS.gene001404:CDS | 35.0% |
| !! | ACTTTTGACTCTATACTTGC+TGG | + | 11231:5353-5372 | MS.gene001404:CDS | 35.0% |
| !! | ATGATTGAGGAGGTGTTAAT+GGG | + | 11231:5497-5516 | MS.gene001404:CDS | 35.0% |
| !! | GGAAGTGCTTAGTGTTATAA+TGG | + | 11231:5280-5299 | MS.gene001404:CDS | 35.0% |
| !! | GTACCATGATGTTTTTGAGA+GGG | - | 11231:4646-4665 | MS.gene001404:intergenic | 35.0% |
| !! | TACCATGATGTTTTTGAGAG+GGG | - | 11231:4645-4664 | MS.gene001404:intergenic | 35.0% |
| !! | TATGATTGAGGAGGTGTTAA+TGG | + | 11231:5496-5515 | MS.gene001404:CDS | 35.0% |
| !! | TGTACCATGATGTTTTTGAG+AGG | - | 11231:4647-4666 | MS.gene001404:intergenic | 35.0% |
| !! | TTTGCAAGTTGGTTGATTGT+GGG | + | 11231:5228-5247 | MS.gene001404:CDS | 35.0% |
| AAAATGCGTGAGGTTGTGAT+TGG | + | 11231:5542-5561 | MS.gene001404:CDS | 40.0% | |
| AACAGTTGAACGACAACCTT+GGG | + | 11231:5010-5029 | MS.gene001404:CDS | 40.0% | |
| AACCCAACATCACTTCCATT+CGG | - | 11231:5596-5615 | MS.gene001404:intergenic | 40.0% | |
| AACCCCTCTCAAAAACATCA+TGG | + | 11231:4640-4659 | MS.gene001404:CDS | 40.0% | |
| ACCACCTGTCAAAAACATGT+CGG | + | 11231:4983-5002 | MS.gene001404:CDS | 40.0% | |
| ATCGATTAGATCAACGGCTA+GGG | - | 11231:4832-4851 | MS.gene001404:intergenic | 40.0% | |
| ATGGAAGTGATGTTGGGTTT+AGG | + | 11231:5597-5616 | MS.gene001404:CDS | 40.0% | |
| ATGTACAAAGTCTACCTTGG+TGG | + | 11231:4853-4872 | MS.gene001404:CDS | 40.0% | |
| CAAAAGAAGAAATGCTGACG+TGG | + | 11231:5084-5103 | MS.gene001404:CDS | 40.0% | |
| GGAAGTTGAAAAAATGCGTG+AGG | + | 11231:5532-5551 | MS.gene001404:CDS | 40.0% | |
| GGTGTTAATGGGAATTAAGG+AGG | + | 11231:5508-5527 | MS.gene001404:CDS | 40.0% | |
| GTGGGAAAAGAAAAGGGTTA+GGG | + | 11231:5156-5175 | MS.gene001404:CDS | 40.0% | |
| GTTAATGGGAATTAAGGAGG+TGG | + | 11231:5511-5530 | MS.gene001404:CDS | 40.0% | |
| TAACAGTTGAACGACAACCT+TGG | + | 11231:5009-5028 | MS.gene001404:CDS | 40.0% | |
| TATTGCGTATACGCTGTATG+TGG | - | 11231:5401-5420 | MS.gene001404:intergenic | 40.0% | |
| TCGGTTTATATTGAAGAGGG+GGG | + | 11231:5455-5474 | MS.gene001404:CDS | 40.0% | |
| TCTCCTTAGGCAAATACCAA+TGG | - | 11231:5424-5443 | MS.gene001404:intergenic | 40.0% | |
| TGAGTCATCTCTTTGCAAGT+TGG | + | 11231:5217-5236 | MS.gene001404:CDS | 40.0% | |
| TGCCCTTATGTGAAGAACAA+TGG | + | 11231:4609-4628 | MS.gene001404:CDS | 40.0% | |
| TGGAAGTGATGTTGGGTTTA+GGG | + | 11231:5598-5617 | MS.gene001404:CDS | 40.0% | |
| TTCTACATACCATTCTACGG+TGG | + | 11231:4756-4775 | MS.gene001404:CDS | 40.0% | |
| ! | CGTGATTTTCTAACCTAGCA+TGG | - | 11231:4701-4720 | MS.gene001404:intergenic | 40.0% |
| ! | TAAGCACTTCCATTGGTTGA+TGG | - | 11231:5271-5290 | MS.gene001404:intergenic | 40.0% |
| !! | ACATGTTTTTGACAGGTGGT+AGG | - | 11231:4983-5002 | MS.gene001404:intergenic | 40.0% |
| !! | CTTTGCAAGTTGGTTGATTG+TGG | + | 11231:5227-5246 | MS.gene001404:CDS | 40.0% |
| !! | GGAGGTGTTAATGGGAATTA+AGG | + | 11231:5505-5524 | MS.gene001404:CDS | 40.0% |
| !! | TGTGATTGGTTTGATCCCTA+GGG | + | 11231:5556-5575 | MS.gene001404:CDS | 40.0% |
| !! | TTGCAAGTTGGTTGATTGTG+GGG | + | 11231:5229-5248 | MS.gene001404:CDS | 40.0% |
| !! | TTGTGATTGGTTTGATCCCT+AGG | + | 11231:5555-5574 | MS.gene001404:CDS | 40.0% |
| ACCCAACATCACTTCCATTC+GGG | - | 11231:5595-5614 | MS.gene001404:intergenic | 45.0% | |
| ACCCGAATGGAAGTGATGTT+GGG | + | 11231:5591-5610 | MS.gene001404:CDS | 45.0% | |
| ATTTCATGCGGTCCTCAAGT+GGG | + | 11231:4928-4947 | MS.gene001404:CDS | 45.0% | |
| CAGCGTATACGCAATACCAT+TGG | + | 11231:5405-5424 | MS.gene001404:CDS | 45.0% | |
| GATCGATTAGATCAACGGCT+AGG | - | 11231:4833-4852 | MS.gene001404:intergenic | 45.0% | |
| GCTGTTGATGTTGCACTTCA+AGG | + | 11231:5623-5642 | MS.gene001404:CDS | 45.0% | |
| GGATGAAAATACGACGGGTA+AGG | - | 11231:5059-5078 | MS.gene001404:intergenic | 45.0% | |
| GGTGGGAAAAGAAAAGGGTT+AGG | + | 11231:5155-5174 | MS.gene001404:CDS | 45.0% | |
| ! | CGTTTTTGTCTTCAAGCACC+AGG | + | 11231:5308-5327 | MS.gene001404:CDS | 45.0% |
| ! | TATATTGAAGAGGGGGGTGT+AGG | + | 11231:5461-5480 | MS.gene001404:CDS | 45.0% |
| !! | ACCGACATGTTTTTGACAGG+TGG | - | 11231:4987-5006 | MS.gene001404:intergenic | 45.0% |
| !! | TGCAAGTTGGTTGATTGTGG+GGG | + | 11231:5230-5249 | MS.gene001404:CDS | 45.0% |
| !!! | AGCACCGACATGTTTTTGAC+AGG | - | 11231:4990-5009 | MS.gene001404:intergenic | 45.0% |
| AAAATCACGTGTGTCGCACG+TGG | + | 11231:4712-4731 | MS.gene001404:CDS | 50.0% | |
| AAATCACGTGTGTCGCACGT+GGG | + | 11231:4713-4732 | MS.gene001404:CDS | 50.0% | |
| AGTTGGTTGATTGTGGGGGT+GGG | + | 11231:5234-5253 | MS.gene001404:CDS | 50.0% | |
| CACCCGAATGGAAGTGATGT+TGG | + | 11231:5590-5609 | MS.gene001404:CDS | 50.0% | |
| CCATTGTGACGATTCCACCA+AGG | - | 11231:4870-4889 | MS.gene001404:intergenic | 50.0% | |
| CCTTGGTGGAATCGTCACAA+TGG | + | 11231:4867-4886 | MS.gene001404:CDS | 50.0% | |
| GATTTCATGCGGTCCTCAAG+TGG | + | 11231:4927-4946 | MS.gene001404:CDS | 50.0% | |
| GGTGCGCATAACTAACCCTA+GGG | - | 11231:5574-5593 | MS.gene001404:intergenic | 50.0% | |
| GTTAGTTATGCGCACCCGAA+TGG | + | 11231:5578-5597 | MS.gene001404:CDS | 50.0% | |
| GTTGAACGACAACCTTGGGA+AGG | + | 11231:5014-5033 | MS.gene001404:CDS | 50.0% | |
| TCCAAAATCGGACCCACTTG+AGG | - | 11231:4943-4962 | MS.gene001404:intergenic | 50.0% | |
| ! | AAGTTGGTTGATTGTGGGGG+TGG | + | 11231:5233-5252 | MS.gene001404:CDS | 50.0% |
| ! | TCCTCAAGTGGGTCCGATTT+TGG | + | 11231:4939-4958 | MS.gene001404:CDS | 50.0% |
| GCGTGTAGTCCACCGTAGAA+TGG | - | 11231:4768-4787 | MS.gene001404:intergenic | 55.0% | |
| GGACCGCATGAAATCCCATG+CGG | - | 11231:4922-4941 | MS.gene001404:intergenic | 55.0% | |
| GGACCGCATGGGATTTCATG+CGG | + | 11231:4916-4935 | MS.gene001404:CDS | 55.0% | |
| GGGTGCGCATAACTAACCCT+AGG | - | 11231:5575-5594 | MS.gene001404:intergenic | 55.0% | |
| ! | TTCTCTGGGAAGGACCGCAT+GGG | + | 11231:4905-4924 | MS.gene001404:CDS | 55.0% |
| ! | TTTCTCTGGGAAGGACCGCA+TGG | + | 11231:4904-4923 | MS.gene001404:CDS | 55.0% |
| CGCTTCAAGCATGTTCCGCG+AGG | + | 11231:4785-4804 | MS.gene001404:CDS | 60.0% | |
| ! | GGAGAGTGTGATTCGCCTCG+CGG | - | 11231:4803-4822 | MS.gene001404:intergenic | 60.0% |
| !! | GATCAACGGCTAGGGCATCG+CGG | - | 11231:4824-4843 | MS.gene001404:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 11231 | gene | 4606 | 5685 | 4606 | ID=MS.gene001404 |
| 11231 | mRNA | 4606 | 5685 | 4606 | ID=MS.gene001404.t1;Parent=MS.gene001404 |
| 11231 | exon | 4606 | 5685 | 4606 | ID=MS.gene001404.t1.exon1;Parent=MS.gene001404.t1 |
| 11231 | CDS | 4606 | 5685 | 4606 | ID=cds.MS.gene001404.t1;Parent=MS.gene001404.t1 |
| Gene Sequence |
| Protein sequence |
