AlfalfaGEDB | MS.gene77538

M. sativa cultivar XinJiangDaYe / MS.gene77538

KEGG: -
GO: -
PFAM: PF03016.18

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77538.t1	XP_003601693.1	98.2	387	7	0	1	387	98	484	4.50E-231	810.1

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77538.t1	Q84R16	57.2	388	162	3	1	387	128	512	6.0e-131	468.8

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77538.t1	G7JAS7	98.2	387	7	0	1	387	98	484	3.3e-231	810.1

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051719	MS.gene77538	0.821415	4.09E-53	-1.69E-46
MS.gene051720	MS.gene77538	0.814735	1.32E-51	-1.69E-46
MS.gene052020	MS.gene77538	0.812397	4.33E-51	-1.69E-46
MS.gene053144	MS.gene77538	0.802315	5.95E-49	-1.69E-46
MS.gene055465	MS.gene77538	0.805702	1.18E-49	-1.69E-46
MS.gene055466	MS.gene77538	0.806242	9.05E-50	-1.69E-46
MS.gene060587	MS.gene77538	0.806841	6.76E-50	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene001404	MS.gene77538	PPI
MS.gene026309	MS.gene77538	PPI
MS.gene035337	MS.gene77538	PPI
MS.gene036481	MS.gene77538	PPI
MS.gene041562	MS.gene77538	PPI
MS.gene067185	MS.gene77538	PPI
MS.gene32944	MS.gene77538	PPI
MS.gene58506	MS.gene77538	PPI
MS.gene77538	MS.gene32945	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77538.t1	MTR_3g084400	98.191	387	7	0	1	387	98	484	0.0	798
MS.gene77538.t1	MTR_7g098650	55.039	387	169	3	1	386	110	492	5.80e-161	463
MS.gene77538.t1	MTR_1g069460	53.488	387	175	3	1	386	185	567	2.21e-157	456
MS.gene77538.t1	MTR_4g129930	56.164	365	157	2	1	363	102	465	4.34e-155	445
MS.gene77538.t1	MTR_5g055350	52.703	370	162	4	1	363	83	446	2.37e-146	422
MS.gene77538.t1	MTR_5g095940	52.419	372	171	3	1	367	125	495	1.83e-140	409
MS.gene77538.t1	MTR_3g007840	50.266	376	158	4	1	371	53	404	1.43e-132	386
MS.gene77538.t1	MTR_5g095950	47.074	376	162	4	1	371	98	441	3.33e-120	357
MS.gene77538.t1	MTR_1g035730	42.340	359	194	3	1	356	103	451	1.53e-94	290
MS.gene77538.t1	MTR_1g102430	42.779	367	194	9	2	360	132	490	1.22e-90	282

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77538.t1	AT4G13990	66.237	388	127	3	1	387	128	512	0.0	548
MS.gene77538.t1	AT4G13990	66.237	388	127	3	1	387	128	512	0.0	548
MS.gene77538.t1	AT2G20370	53.230	387	177	2	1	386	182	565	2.60e-158	459
MS.gene77538.t1	AT2G32750	53.333	375	169	3	1	371	114	486	5.14e-149	431
MS.gene77538.t1	AT2G32750	53.333	375	169	3	1	371	116	488	5.67e-149	431
MS.gene77538.t1	AT2G29040	53.213	389	171	4	1	384	330	712	1.05e-145	431
MS.gene77538.t1	AT2G31990	52.830	371	163	7	1	363	114	480	4.07e-140	408
MS.gene77538.t1	AT2G31990	52.830	371	163	7	1	363	106	472	5.46e-140	407
MS.gene77538.t1	AT2G31990	52.815	373	160	8	1	363	152	518	1.74e-139	408
MS.gene77538.t1	AT2G32740	50.000	364	175	4	2	359	98	460	1.52e-131	385

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Find 73 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACATCATTATCCATTGA+AGG	0.431263	3.2:-72690540	None:intergenic
AAAATGAAGATTCACTTAGG+GGG	0.656953	3.2:+72690634	MS.gene77538:CDS
AAAATTATTGAACAGTGTCG+TGG	0.616891	3.2:+72690657	MS.gene77538:CDS
AAACCCATGTTGTCCATGTA+CGG	0.517484	3.2:-72690051	None:intergenic
AAAGGGAAAGATCCTAGTGT+TGG	0.572150	3.2:+72691146	MS.gene77538:CDS
AAATAACCTCCAACAGAAAC+TGG	0.384297	3.2:-72690143	None:intergenic
AAATAGTTTATGCAGATCCT+AGG	0.516398	3.2:+72691036	MS.gene77538:CDS
AAATTATCTAACTTAGACCT+AGG	0.558142	3.2:-72691053	None:intergenic
AAATTCATTGATTGTAGTTA+TGG	0.414533	3.2:+72690693	MS.gene77538:CDS
AACACCTTCATAACATTAAC+AGG	0.333473	3.2:-72690735	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATGAAGATTCACTTAGG+GGG	+	chr3.2:72690634-72690653	MS.gene77538:CDS	30.0%
AAAATTATTGAACAGTGTCG+TGG	+	chr3.2:72690657-72690676	MS.gene77538:CDS	30.0%
AAATAGTTTATGCAGATCCT+AGG	+	chr3.2:72691036-72691055	MS.gene77538:CDS	30.0%
AACACCTTCATAACATTAAC+AGG	-	chr3.2:72690738-72690757	None:intergenic	30.0%
AACTTCATTTCATCCTTCAA+TGG	+	chr3.2:72690527-72690546	MS.gene77538:CDS	30.0%
ATTGAAGGATGAAATGAAGT+TGG	-	chr3.2:72690528-72690547	None:intergenic	30.0%
CAGCTTATTCACAATATAAG+TGG	+	chr3.2:72690865-72690884	MS.gene77538:CDS	30.0%
CTGAAAATGAAGATTCACTT+AGG	+	chr3.2:72690631-72690650	MS.gene77538:CDS	30.0%
GAAAATGAAGATTCACTTAG+GGG	+	chr3.2:72690633-72690652	MS.gene77538:CDS	30.0%
TGAAAGGATTGAGAATGTAA+GGG	+	chr3.2:72691112-72691131	MS.gene77538:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	72690045	72691208	72690045	ID=MS.gene77538
chr3.2	mRNA	72690045	72691208	72690045	ID=MS.gene77538.t1;Parent=MS.gene77538
chr3.2	exon	72690045	72691208	72690045	ID=MS.gene77538.t1.exon1;Parent=MS.gene77538.t1
chr3.2	CDS	72690045	72691208	72690045	ID=cds.MS.gene77538.t1;Parent=MS.gene77538.t1

Gene Sequence

>MS.gene77538

ATGTGTCCGTACATGGACAACATGGGTTTAGGTCCTGAGGTTAAAAATCAAAACTTTAAGGAAATTCTTGTCCCAAACAATACTTGGTATGCTACAAACCAGTTTCTGTTGGAGGTTATTTTTCACAATAGGATGAAAAGCTATGAATGTTTGACAAATGATTCATCTTTAGCTTCTGCAGTTTTTGTTCCTTCTTATATTGGTCTTGATATTAGTCGTTTTCTTTGGGTTAATAATCTTACTGTGAGAGATTCATCTGGTTTTGAACTTGTTAATTGGTTGGTTGAAAAACCTGAGTGGAAGAAAATGTGGGGTAGGGATCATTTTCTTATTTCTGGAAGAATTTCTTGGGATTTTAGAAGACAATTTGATGATTTGGCTTATTGGGGTAGTAAGTTTAGGTTTTTACCTGAATCTATGAATATGTCAATGTTAGCAGTTGAAGGTAGTTCATGGAATAATGATTATGCTATACCTTATCCAACTTCATTTCATCCTTCAATGGATAATGATGTTTTGCAATGGCAGAGTAAAATTAGACATCAAAAGAGGGAATTTTTGTTTACTTTCACTGGAGCACCAAGGCCTGAAAATGAAGATTCACTTAGGGGGAAAATTATTGAACAGTGTCGTGGTTCGAGGTTTTGCAAATTCATTGATTGTAGTTATGGTGGAGAGAAATGTGATGATCCTGTTAATGTTATGAAGGTGTTTGGAAATTCTGTGTTCTCTTTGCAACCTTCTGGTGATTCATACACAAGAAGATCAATTTTTGATTCAATTTTAGCTGGTTGTATTCCTGTGTTTTTCCATCCTGGTACAGCTTATTCACAATATAAGTGGCATTTACCAAAAAACAGAACTAAGTATTCTGTTTATATACCTGTGAAGGATGTGAAAGAATGGAATGTTGATCTTGAGAAGGTGTTGTTGGCAATTCCAGAGAAGGAGGTTATTGCAATGAGAGAAGAGGTTATAGAGCTTATTCCGAAAATAGTTTATGCAGATCCTAGGTCTAAGTTAGATAATTTTGAAGATGCATTTGATTTAGCATTGAAAGGGATGCTTGAAAGGATTGAGAATGTAAGGGAAACAGTGAGGAAAGGGAAAGATCCTAGTGTTGGTTTTGCAGATGAAGATCATTATAAGTATACATTTAATTAG

Protein sequence

>MS.gene77538.t1

MCPYMDNMGLGPEVKNQNFKEILVPNNTWYATNQFLLEVIFHNRMKSYECLTNDSSLASAVFVPSYIGLDISRFLWVNNLTVRDSSGFELVNWLVEKPEWKKMWGRDHFLISGRISWDFRRQFDDLAYWGSKFRFLPESMNMSMLAVEGSSWNNDYAIPYPTSFHPSMDNDVLQWQSKIRHQKREFLFTFTGAPRPENEDSLRGKIIEQCRGSRFCKFIDCSYGGEKCDDPVNVMKVFGNSVFSLQPSGDSYTRRSIFDSILAGCIPVFFHPGTAYSQYKWHLPKNRTKYSVYIPVKDVKEWNVDLEKVLLAIPEKEVIAMREEVIELIPKIVYADPRSKLDNFEDAFDLALKGMLERIENVRETVRKGKDPSVGFADEDHYKYTFN