Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035337.t1 | XP_003609665.1 | 98 | 356 | 7 | 0 | 1 | 356 | 100 | 455 | 4.50E-209 | 736.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035337.t1 | Q9SUW1 | 67.0 | 349 | 107 | 2 | 1 | 349 | 95 | 435 | 2.2e-140 | 500.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035337.t1 | G7JLG7 | 98.0 | 356 | 7 | 0 | 1 | 356 | 100 | 455 | 3.2e-209 | 736.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene035337 | MS.gene067185 | PPI |
| MS.gene035337 | MS.gene001404 | PPI |
| MS.gene035337 | MS.gene79900 | PPI |
| MS.gene035337 | MS.gene026309 | PPI |
| MS.gene035337 | MS.gene32944 | PPI |
| MS.gene035337 | MS.gene75663 | PPI |
| MS.gene035337 | MS.gene77538 | PPI |
| MS.gene035337 | MS.gene045350 | PPI |
| MS.gene035337 | MS.gene32945 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035337.t1 | MTR_4g119810 | 98.034 | 356 | 7 | 0 | 1 | 356 | 100 | 455 | 0.0 | 730 |
| MS.gene035337.t1 | MTR_7g098650 | 40.698 | 344 | 184 | 9 | 2 | 345 | 143 | 466 | 2.28e-72 | 234 |
| MS.gene035337.t1 | MTR_3g084400 | 39.255 | 349 | 187 | 9 | 1 | 347 | 133 | 458 | 7.62e-71 | 229 |
| MS.gene035337.t1 | MTR_1g069460 | 39.826 | 344 | 187 | 9 | 2 | 345 | 218 | 541 | 5.95e-70 | 230 |
| MS.gene035337.t1 | MTR_5g055350 | 39.316 | 351 | 180 | 10 | 2 | 345 | 118 | 442 | 6.58e-69 | 223 |
| MS.gene035337.t1 | MTR_1g102430 | 36.207 | 348 | 201 | 6 | 3 | 350 | 168 | 494 | 1.69e-67 | 221 |
| MS.gene035337.t1 | MTR_4g129930 | 38.395 | 349 | 190 | 9 | 2 | 347 | 137 | 463 | 1.20e-66 | 218 |
| MS.gene035337.t1 | MTR_5g095940 | 36.034 | 358 | 203 | 7 | 2 | 354 | 160 | 496 | 1.01e-64 | 213 |
| MS.gene035337.t1 | MTR_5g010370 | 32.659 | 346 | 209 | 8 | 2 | 345 | 219 | 542 | 4.49e-60 | 202 |
| MS.gene035337.t1 | MTR_5g010350 | 34.375 | 352 | 206 | 9 | 2 | 351 | 195 | 523 | 1.26e-58 | 198 |
| MS.gene035337.t1 | MTR_3g007840 | 34.270 | 356 | 185 | 7 | 2 | 352 | 88 | 399 | 3.09e-58 | 194 |
| MS.gene035337.t1 | MTR_1g035730 | 34.593 | 344 | 199 | 9 | 1 | 340 | 128 | 449 | 2.49e-55 | 187 |
| MS.gene035337.t1 | MTR_5g095950 | 29.775 | 356 | 193 | 10 | 2 | 352 | 133 | 436 | 2.33e-40 | 149 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035337.t1 | AT4G22580 | 69.341 | 349 | 99 | 2 | 1 | 349 | 95 | 435 | 3.65e-178 | 501 |
| MS.gene035337.t1 | AT2G29040 | 40.282 | 355 | 189 | 8 | 2 | 351 | 360 | 696 | 1.01e-77 | 253 |
| MS.gene035337.t1 | AT4G13990 | 40.462 | 346 | 184 | 7 | 1 | 345 | 160 | 484 | 1.51e-76 | 245 |
| MS.gene035337.t1 | AT4G13990 | 40.462 | 346 | 184 | 7 | 1 | 345 | 160 | 484 | 1.51e-76 | 245 |
| MS.gene035337.t1 | AT2G32750 | 37.391 | 345 | 194 | 9 | 1 | 345 | 154 | 476 | 1.07e-71 | 232 |
| MS.gene035337.t1 | AT2G32750 | 37.391 | 345 | 194 | 9 | 1 | 345 | 152 | 474 | 1.36e-71 | 231 |
| MS.gene035337.t1 | AT5G62220 | 37.356 | 348 | 197 | 6 | 1 | 348 | 184 | 510 | 1.04e-70 | 229 |
| MS.gene035337.t1 | AT2G31990 | 38.571 | 350 | 192 | 9 | 1 | 345 | 137 | 468 | 9.62e-70 | 226 |
| MS.gene035337.t1 | AT2G31990 | 38.571 | 350 | 192 | 9 | 1 | 345 | 145 | 476 | 1.21e-69 | 226 |
| MS.gene035337.t1 | AT2G31990 | 38.571 | 350 | 192 | 9 | 1 | 345 | 183 | 514 | 3.03e-69 | 226 |
| MS.gene035337.t1 | AT2G20370 | 38.857 | 350 | 193 | 10 | 2 | 351 | 215 | 543 | 5.82e-69 | 227 |
| MS.gene035337.t1 | AT2G32740 | 35.159 | 347 | 201 | 10 | 1 | 345 | 136 | 460 | 7.75e-63 | 207 |
| MS.gene035337.t1 | AT5G41250 | 33.815 | 346 | 202 | 9 | 2 | 345 | 236 | 556 | 4.93e-61 | 205 |
| MS.gene035337.t1 | AT1G63450 | 33.913 | 345 | 205 | 7 | 2 | 345 | 338 | 660 | 6.44e-58 | 199 |
| MS.gene035337.t1 | AT1G68470 | 31.728 | 353 | 210 | 10 | 1 | 349 | 126 | 451 | 1.80e-54 | 185 |
Find 0 sgRNAs with CRISPR-Local
Find 100 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAACTATCAAATTCAATCTC+CGG | - | 32602:15529-15548 | None:intergenic | 25.0% |
| !! | ATTGGTTTCATAAACCTAAT+TGG | - | 32602:15370-15389 | None:intergenic | 25.0% |
| !! | TCATGATCATTTTCTTGTTA+TGG | + | 32602:14946-14965 | MS.gene035337:CDS | 25.0% |
| !! | TTTTTCTGTTACTATACCTA+AGG | + | 32602:15546-15565 | MS.gene035337:CDS | 25.0% |
| AACAATAGCAGTAATGTGAA+TGG | + | 32602:15277-15296 | MS.gene035337:CDS | 30.0% | |
| AAGAAAATCACCCTGAAATA+TGG | + | 32602:14909-14928 | MS.gene035337:CDS | 30.0% | |
| AATGTGAATGGGAATTCATT+TGG | + | 32602:15289-15308 | MS.gene035337:CDS | 30.0% | |
| ACAATAGCAGTAATGTGAAT+GGG | + | 32602:15278-15297 | MS.gene035337:CDS | 30.0% | |
| AGCATGGATATTCTAACATA+CGG | - | 32602:14754-14773 | None:intergenic | 30.0% | |
| AGGTTTATGAAACCAATGTT+GGG | + | 32602:15373-15392 | MS.gene035337:CDS | 30.0% | |
| GTTAGAATATCCATGCTTAA+CGG | + | 32602:14757-14776 | MS.gene035337:CDS | 30.0% | |
| TAGGTTTATGAAACCAATGT+TGG | + | 32602:15372-15391 | MS.gene035337:CDS | 30.0% | |
| ! | GAAACTGTTGATTGTTCTAA+TGG | + | 32602:15328-15347 | MS.gene035337:CDS | 30.0% |
| ! | GCATTTGATTTAACAATCGA+CGG | + | 32602:15724-15743 | MS.gene035337:CDS | 30.0% |
| ! | TATTTCAGGGTGATTTTCTT+TGG | - | 32602:14909-14928 | None:intergenic | 30.0% |
| AATGACGGTTCCATATTTCA+GGG | - | 32602:14922-14941 | None:intergenic | 35.0% | |
| AATTTCTGTTTACAACCACC+AGG | + | 32602:15400-15419 | MS.gene035337:CDS | 35.0% | |
| AGATGAGGATCATTATCCAA+AGG | - | 32602:14998-15017 | None:intergenic | 35.0% | |
| AGCGTAGTAAGGAAGATAAA+CGG | - | 32602:14804-14823 | None:intergenic | 35.0% | |
| ATTCAGGACCGTAAAGATAA+CGG | - | 32602:14841-14860 | None:intergenic | 35.0% | |
| ATTTCTGTTTACAACCACCA+GGG | + | 32602:15401-15420 | MS.gene035337:CDS | 35.0% | |
| CTTTGGATAAGATTCGATCT+AGG | + | 32602:15749-15768 | MS.gene035337:CDS | 35.0% | |
| GATAAATCCTCGAAAAACAC+AGG | - | 32602:15478-15497 | None:intergenic | 35.0% | |
| GATAATGATCCTCATCTTTG+GGG | + | 32602:15001-15020 | MS.gene035337:CDS | 35.0% | |
| GATTCTCTCCGTTATCTTTA+CGG | + | 32602:14830-14849 | MS.gene035337:CDS | 35.0% | |
| GCGGTTACATTAAAAAACTG+TGG | - | 32602:15046-15065 | None:intergenic | 35.0% | |
| GGATAATGATCCTCATCTTT+GGG | + | 32602:15000-15019 | MS.gene035337:CDS | 35.0% | |
| TAATGTAACCGCGTTAACTT+TGG | + | 32602:15054-15073 | MS.gene035337:CDS | 35.0% | |
| TAGGCTTCAAGAACTTGATT+TGG | + | 32602:15768-15787 | MS.gene035337:CDS | 35.0% | |
| TATAACTCCAGTGAACAACA+CGG | + | 32602:14860-14879 | MS.gene035337:CDS | 35.0% | |
| TGAAATATGGAACCGTCATT+CGG | + | 32602:14922-14941 | MS.gene035337:CDS | 35.0% | |
| TGATTCCTGGAGTTGTTTAT+AGG | + | 32602:15662-15681 | MS.gene035337:CDS | 35.0% | |
| TGGATAATGATCCTCATCTT+TGG | + | 32602:14999-15018 | MS.gene035337:CDS | 35.0% | |
| TGTTCACTGGAGTTATATTC+AGG | - | 32602:14857-14876 | None:intergenic | 35.0% | |
| TTCTGTTACTATACCTAAGG+AGG | + | 32602:15549-15568 | MS.gene035337:CDS | 35.0% | |
| TTGAAAACAACATCCTCCTT+AGG | - | 32602:15565-15584 | None:intergenic | 35.0% | |
| TTTAACAATCGACGGTACTT+TGG | + | 32602:15732-15751 | MS.gene035337:CDS | 35.0% | |
| TTTGTGAGCATGATCCAATT+AGG | + | 32602:15353-15372 | MS.gene035337:CDS | 35.0% | |
| ! | TCCTGGTTTGAGAAATAAGA+AGG | + | 32602:15699-15718 | MS.gene035337:CDS | 35.0% |
| ! | TCCTTCTTATTTCTCAAACC+AGG | - | 32602:15703-15722 | None:intergenic | 35.0% |
| ! | TTGTATACCTGTGTTTTTCG+AGG | + | 32602:15468-15487 | MS.gene035337:CDS | 35.0% |
| !! | AAGATAACGGAGAGAATCAA+AGG | - | 32602:14828-14847 | None:intergenic | 35.0% |
| !!! | AAAGTTTTGGAGTTGATTCC+TGG | + | 32602:15649-15668 | MS.gene035337:CDS | 35.0% |
| !!! | AAGGAGGATGTTGTTTTCAA+GGG | + | 32602:15565-15584 | MS.gene035337:CDS | 35.0% |
| !!! | TAAGGAGGATGTTGTTTTCA+AGG | + | 32602:15564-15583 | MS.gene035337:CDS | 35.0% |
| AAGAGCAAGAGAAGATCTCT+TGG | - | 32602:15185-15204 | None:intergenic | 40.0% | |
| AATGATCATGACCCGAATGA+CGG | - | 32602:14937-14956 | None:intergenic | 40.0% | |
| ACGAGATTCCAAAGTTAACG+CGG | - | 32602:15065-15084 | None:intergenic | 40.0% | |
| ATTCCATTCTTATACTCCGC+CGG | - | 32602:15246-15265 | None:intergenic | 40.0% | |
| CAGAAATTTGCACCCAACAT+TGG | - | 32602:15388-15407 | None:intergenic | 40.0% | |
| GAAATATGGAACCGTCATTC+GGG | + | 32602:14923-14942 | MS.gene035337:CDS | 40.0% | |
| GAATCCAACAACGCTAGATT+CGG | - | 32602:15139-15158 | None:intergenic | 40.0% | |
| GAATGACGGTTCCATATTTC+AGG | - | 32602:14923-14942 | None:intergenic | 40.0% | |
| GATATTCTAACATACGGCGA+TGG | - | 32602:14748-14767 | None:intergenic | 40.0% | |
| TGCTTCCTATAAACAACTCC+AGG | - | 32602:15670-15689 | None:intergenic | 40.0% | |
| TTAGGATCTTCCGTTAAGCA+TGG | - | 32602:14770-14789 | None:intergenic | 40.0% | |
| TTTCTGCTACGCCAAATATC+CGG | + | 32602:15224-15243 | MS.gene035337:CDS | 40.0% | |
| TTTCTGTTTACAACCACCAG+GGG | + | 32602:15402-15421 | MS.gene035337:CDS | 40.0% | |
| ! | AGGAAGCATAATAGTTCTCC+TGG | + | 32602:15682-15701 | MS.gene035337:CDS | 40.0% |
| ! | GAGAATGAGGGAGAAAGTTT+TGG | + | 32602:15636-15655 | MS.gene035337:CDS | 40.0% |
| ! | TCAAAAGTGGATTTACGCGT+AGG | - | 32602:15433-15452 | None:intergenic | 40.0% |
| ! | TCTTCTCTTGCTCTTTTTGC+AGG | + | 32602:15190-15209 | MS.gene035337:CDS | 40.0% |
| !! | TAACTTTGGAATCTCGTGCT+TGG | + | 32602:15068-15087 | MS.gene035337:CDS | 40.0% |
| AACGAAGTTCCCCAAAGATG+AGG | - | 32602:15013-15032 | None:intergenic | 45.0% | |
| AGCTAGGGTTAGGAGAATGA+GGG | + | 32602:15624-15643 | MS.gene035337:CDS | 45.0% | |
| AGGAAGATAAACGGCGTTAG+CGG | - | 32602:14795-14814 | None:intergenic | 45.0% | |
| ATCTAGCGTTGTTGGATTCG+TGG | + | 32602:15140-15159 | MS.gene035337:CDS | 45.0% | |
| CTGCTAAATCGCAGTATTCG+TGG | + | 32602:15497-15516 | MS.gene035337:CDS | 45.0% | |
| CTTATACTCCGCCGGATATT+TGG | - | 32602:15238-15257 | None:intergenic | 45.0% | |
| GAATACCTAGAGCTAGGGTT+AGG | + | 32602:15614-15633 | MS.gene035337:CDS | 45.0% | |
| GAATCAAAGGCAGCGTAGTA+AGG | - | 32602:14815-14834 | None:intergenic | 45.0% | |
| GCAGAGAATACCTAGAGCTA+GGG | + | 32602:15609-15628 | MS.gene035337:CDS | 45.0% | |
| GCAGTATTCGTGGCATTTAC+CGG | + | 32602:15507-15526 | MS.gene035337:CDS | 45.0% | |
| GGATGAAACGACGTTGGATA+AGG | - | 32602:15115-15134 | None:intergenic | 45.0% | |
| TATCCGGCGGAGTATAAGAA+TGG | + | 32602:15240-15259 | MS.gene035337:CDS | 45.0% | |
| TGCAAGAATCGCGTCAAAAG+TGG | - | 32602:15446-15465 | None:intergenic | 45.0% | |
| TGCAGAGAATACCTAGAGCT+AGG | + | 32602:15608-15627 | MS.gene035337:CDS | 45.0% | |
| TTGGGATTTCTCTCAGCCTT+TGG | + | 32602:14979-14998 | MS.gene035337:CDS | 45.0% | |
| ! | ACTTTTGACGCGATTCTTGC+AGG | + | 32602:15445-15464 | MS.gene035337:CDS | 45.0% |
| ! | ATGAACACCGTGTTGTTCAC+TGG | - | 32602:14870-14889 | None:intergenic | 45.0% |
| ! | ATTCTCCTAACCCTAGCTCT+AGG | - | 32602:15622-15641 | None:intergenic | 45.0% |
| ! | TAAACGGCGTTAGCGGTTTT+AGG | - | 32602:14788-14807 | None:intergenic | 45.0% |
| ! | TCTCTTGCTCTTTTTGCAGG+TGG | + | 32602:15193-15212 | MS.gene035337:CDS | 45.0% |
| AGGCTGAGAGAAATCCCAAG+CGG | - | 32602:14978-14997 | None:intergenic | 50.0% | |
| GAGCTAGGGTTAGGAGAATG+AGG | + | 32602:15623-15642 | MS.gene035337:CDS | 50.0% | |
| GGAACAGCATGTTCTTGCCA+CGG | - | 32602:15094-15113 | None:intergenic | 50.0% | |
| TCCAACAACGCTAGATTCGG+TGG | - | 32602:15136-15155 | None:intergenic | 50.0% | |
| TCCACCGAATCTAGCGTTGT+TGG | + | 32602:15132-15151 | MS.gene035337:CDS | 50.0% | |
| TTCGGTGGATGAAACGACGT+TGG | - | 32602:15121-15140 | None:intergenic | 50.0% | |
| ! | CTTGCTCTTTTTGCAGGTGG+TGG | + | 32602:15196-15215 | MS.gene035337:CDS | 50.0% |
| AGAGAAATCCCAAGCGGGTC+GGG | - | 32602:14972-14991 | None:intergenic | 55.0% | |
| CTGCTACGCCAAATATCCGG+CGG | + | 32602:15227-15246 | MS.gene035337:CDS | 55.0% | |
| GAGAGAAATCCCAAGCGGGT+CGG | - | 32602:14973-14992 | None:intergenic | 55.0% | |
| GGCTGAGAGAAATCCCAAGC+GGG | - | 32602:14977-14996 | None:intergenic | 55.0% | |
| !! | GCTCTTTTTGCAGGTGGTGG+TGG | + | 32602:15199-15218 | MS.gene035337:CDS | 55.0% |
| !! | TTACGCGTAGGAGTATCCCC+TGG | - | 32602:15421-15440 | None:intergenic | 55.0% |
| TGGAATCTCGTGCTTGGCCG+TGG | + | 32602:15074-15093 | MS.gene035337:CDS | 60.0% | |
| TGTTATGGCCCGACCCGCTT+GGG | + | 32602:14961-14980 | MS.gene035337:CDS | 60.0% | |
| TTGTTATGGCCCGACCCGCT+TGG | + | 32602:14960-14979 | MS.gene035337:CDS | 60.0% | |
| !! | CGCGTAGGAGTATCCCCTGG+TGG | - | 32602:15418-15437 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 32602 | gene | 14728 | 15798 | 14728 | ID=MS.gene035337 |
| 32602 | mRNA | 14728 | 15798 | 14728 | ID=MS.gene035337.t1;Parent=MS.gene035337 |
| 32602 | exon | 14728 | 15798 | 14728 | ID=MS.gene035337.t1.exon1;Parent=MS.gene035337.t1 |
| 32602 | CDS | 14728 | 15798 | 14728 | ID=cds.MS.gene035337.t1;Parent=MS.gene035337.t1 |
| Gene Sequence |
| Protein sequence |
