Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00309.t1 | XP_013463254.1 | 100 | 81 | 0 | 0 | 1 | 81 | 1 | 81 | 5.00E-39 | 169.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00309.t1 | Q3E902 | 85.2 | 81 | 12 | 0 | 1 | 81 | 1 | 81 | 5.8e-36 | 151.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00309.t1 | B7FGU9 | 100.0 | 81 | 0 | 0 | 1 | 81 | 1 | 81 | 3.6e-39 | 169.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene30990 | MS.gene00309 | PPI |
| MS.gene023820 | MS.gene00309 | PPI |
| MS.gene27668 | MS.gene00309 | PPI |
| MS.gene073358 | MS.gene00309 | PPI |
| MS.gene049306 | MS.gene00309 | PPI |
| MS.gene52188 | MS.gene00309 | PPI |
| MS.gene52415 | MS.gene00309 | PPI |
| MS.gene004569 | MS.gene00309 | PPI |
| MS.gene013335 | MS.gene00309 | PPI |
| MS.gene23040 | MS.gene00309 | PPI |
| MS.gene06433 | MS.gene00309 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00309.t1 | MTR_2g436800 | 100.000 | 81 | 0 | 0 | 1 | 81 | 1 | 81 | 4.97e-58 | 172 |
| MS.gene00309.t1 | MTR_7g076530 | 93.827 | 81 | 5 | 0 | 1 | 81 | 1 | 81 | 1.73e-55 | 166 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene00309.t1 | AT5G27700 | 85.185 | 81 | 12 | 0 | 1 | 81 | 1 | 81 | 9.02e-51 | 154 |
| MS.gene00309.t1 | AT3G53890 | 80.247 | 81 | 16 | 0 | 1 | 81 | 1 | 81 | 1.61e-48 | 148 |
| MS.gene00309.t1 | AT3G53890 | 80.247 | 81 | 16 | 0 | 1 | 81 | 1 | 81 | 1.61e-48 | 148 |
Find 18 sgRNAs with CRISPR-Local
Find 87 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAGCTGAACATAGGTCATT+TGG | 0.363523 | 2.1:-56461921 | MS.gene00309:CDS |
| GTTTCAGTTCTGCTACAAAC+AGG | 0.431981 | 2.1:-56461973 | MS.gene00309:intron |
| CTGTGGCTTCACCCGTGCTC+AGG | 0.453938 | 2.1:-56461855 | MS.gene00309:intron |
| AAAAGTTCATACCACTTCCT+AGG | 0.458041 | 2.1:+56462071 | None:intergenic |
| TCACCGAGCTCTACATTCCT+AGG | 0.480407 | 2.1:-56462088 | MS.gene00309:CDS |
| GAAGCCACAGAGAGCAAAAG+TGG | 0.501571 | 2.1:+56461868 | None:intergenic |
| CTAAAGATGCAGAACGAAGA+AGG | 0.519629 | 2.1:-56462116 | None:intergenic |
| ACAGTGGAATTGATCGCCTA+TGG | 0.527769 | 2.1:-56460441 | MS.gene00309:CDS |
| AGCTCTACATTCCTAGGAAG+TGG | 0.545691 | 2.1:-56462082 | MS.gene00309:intron |
| TTGTGTCTACTGCTTAAGCT+CGG | 0.554847 | 2.1:+56460405 | None:intergenic |
| AAACAGGCTTATTACTGCTA+AGG | 0.565079 | 2.1:-56461957 | MS.gene00309:CDS |
| GCATCAGTTCAGCTGAACAT+AGG | 0.573016 | 2.1:-56461929 | MS.gene00309:CDS |
| GATGAGAGCGGTGTTTACAA+TGG | 0.604206 | 2.1:-56461899 | MS.gene00309:CDS |
| ACAAATGGATACCTGAGCAC+GGG | 0.612936 | 2.1:+56461844 | None:intergenic |
| TTTCAGGGAGATGCTGACAG+TGG | 0.628229 | 2.1:-56460457 | MS.gene00309:intron |
| CTTCCTAGGAATGTAGAGCT+CGG | 0.633169 | 2.1:+56462085 | None:intergenic |
| AACAAATGGATACCTGAGCA+CGG | 0.637714 | 2.1:+56461843 | None:intergenic |
| ATAGGTCATTTGGATGAGAG+CGG | 0.690184 | 2.1:-56461911 | MS.gene00309:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTAAGAAATTATGTTATAT+TGG | + | chr2.1:56461068-56461087 | None:intergenic | 10.0% |
| !!! | AAAATAAGTTTTTAATAAAC+AGG | + | chr2.1:56461433-56461452 | None:intergenic | 10.0% |
| !!! | GTTTATTAAAAACTTATTTT+GGG | - | chr2.1:56461433-56461452 | MS.gene00309:intron | 10.0% |
| !!! | TGTTTATTAAAAACTTATTT+TGG | - | chr2.1:56461432-56461451 | MS.gene00309:intron | 10.0% |
| !! | GAATTAAGAGTAAATAATTA+GGG | - | chr2.1:56460729-56460748 | MS.gene00309:intron | 15.0% |
| !! | TATACCAATATAAGATATAT+TGG | + | chr2.1:56461404-56461423 | None:intergenic | 15.0% |
| !! | TATACCAATATATCTTATAT+TGG | - | chr2.1:56461397-56461416 | MS.gene00309:intron | 15.0% |
| !! | TGAATTAAGAGTAAATAATT+AGG | - | chr2.1:56460728-56460747 | MS.gene00309:intron | 15.0% |
| !! | TGATTCTATATATCTTATAT+TGG | - | chr2.1:56461338-56461357 | MS.gene00309:intron | 15.0% |
| !! | TTTCATAAGCTAAAAAAATA+AGG | - | chr2.1:56461113-56461132 | MS.gene00309:intron | 15.0% |
| !! | AAAAATTAGAGTGAAACAAA+TGG | + | chr2.1:56460695-56460714 | None:intergenic | 20.0% |
| !! | AAGTTTACATATTATCAGTA+TGG | - | chr2.1:56461677-56461696 | MS.gene00309:intron | 20.0% |
| !! | ACTTAAAATTTATACTTCCT+CGG | - | chr2.1:56461511-56461530 | MS.gene00309:intron | 20.0% |
| !! | TACTGATAATATGTAAACTT+AGG | + | chr2.1:56461677-56461696 | None:intergenic | 20.0% |
| !!! | ACCATTTGTTTCTTATAAAA+AGG | + | chr2.1:56461539-56461558 | None:intergenic | 20.0% |
| !!! | ACGAATAGTTTTTAATAAAC+AGG | + | chr2.1:56461370-56461389 | None:intergenic | 20.0% |
| !!! | TCCTTTTTATAAGAAACAAA+TGG | - | chr2.1:56461535-56461554 | MS.gene00309:intron | 20.0% |
| !!! | TTTGTTTCACTCTAATTTTT+TGG | - | chr2.1:56460695-56460714 | MS.gene00309:intron | 20.0% |
| ! | AACCAAATACATCAACTTTA+TGG | - | chr2.1:56461593-56461612 | MS.gene00309:intron | 25.0% |
| ! | AGCTAAAAAAATAAGGCAAA+CGG | - | chr2.1:56461120-56461139 | MS.gene00309:intron | 25.0% |
| ! | ATAATATGTAAACTTAGGCA+AGG | + | chr2.1:56461672-56461691 | None:intergenic | 25.0% |
| ! | CTTAAAATTTATACTTCCTC+GGG | - | chr2.1:56461512-56461531 | MS.gene00309:intron | 25.0% |
| ! | GAAAACCTTTATATCTATGT+TGG | - | chr2.1:56461971-56461990 | MS.gene00309:intron | 25.0% |
| ! | TATGTCCAACATAGATATAA+AGG | + | chr2.1:56461979-56461998 | None:intergenic | 25.0% |
| !! | GTGGTATGAACTTTTATAAT+CGG | - | chr2.1:56460458-56460477 | MS.gene00309:intron | 25.0% |
| !!! | TGATGCTTGTTTTATTTTCA+GGG | - | chr2.1:56462049-56462068 | MS.gene00309:intron | 25.0% |
| !!! | TTGATGCTTGTTTTATTTTC+AGG | - | chr2.1:56462048-56462067 | MS.gene00309:intron | 25.0% |
| !!! | TTTGAACTGTTTTTGTTTTG+TGG | - | chr2.1:56460521-56460540 | MS.gene00309:intron | 25.0% |
| AAAAGAGTGAAGACTCAATT+TGG | + | chr2.1:56461261-56461280 | None:intergenic | 30.0% | |
| AGAAACACACTGAAATAAGT+AGG | - | chr2.1:56461939-56461958 | MS.gene00309:CDS | 30.0% | |
| GAAACACACTGAAATAAGTA+GGG | - | chr2.1:56461940-56461959 | MS.gene00309:CDS | 30.0% | |
| GTATTATTCATAAGCTTGTC+AGG | - | chr2.1:56461013-56461032 | MS.gene00309:intron | 30.0% | |
| TAAGAAACAAATGGTTCAAC+AGG | - | chr2.1:56461544-56461563 | MS.gene00309:intron | 30.0% | |
| TATTAATTCATGTCATGCCT+TGG | - | chr2.1:56462006-56462025 | MS.gene00309:intron | 30.0% | |
| TCAAATTTCTCAGCTTACAT+TGG | + | chr2.1:56461737-56461756 | None:intergenic | 30.0% | |
| TCAACAGGAGTTAATGTATT+TGG | - | chr2.1:56461559-56461578 | MS.gene00309:intron | 30.0% | |
| ! | TAAACCAGAACTACTTGTTT+TGG | - | chr2.1:56461166-56461185 | MS.gene00309:intron | 30.0% |
| !! | GACCATAAAGTTGATGTATT+TGG | + | chr2.1:56461598-56461617 | None:intergenic | 30.0% |
| !! | GTTCTGGTTTAATTCTTTGT+CGG | + | chr2.1:56461157-56461176 | None:intergenic | 30.0% |
| !! | TTCTGGTTTAATTCTTTGTC+GGG | + | chr2.1:56461156-56461175 | None:intergenic | 30.0% |
| AAAAGTTCATACCACTTCCT+AGG | + | chr2.1:56460453-56460472 | None:intergenic | 35.0% | |
| AAACAGGCTTATTACTGCTA+AGG | - | chr2.1:56460564-56460583 | MS.gene00309:intron | 35.0% | |
| AAGACAAGCAAAGAAACACA+AGG | + | chr2.1:56461465-56461484 | None:intergenic | 35.0% | |
| TTTCTTATAAAAAGGACCCG+AGG | + | chr2.1:56461531-56461550 | None:intergenic | 35.0% | |
| !! | ACTCGTCAAAGTATTTTCCA+AGG | + | chr2.1:56462026-56462045 | None:intergenic | 35.0% |
| AAACCCAGAACATGTGTGTA+TGG | + | chr2.1:56460828-56460847 | None:intergenic | 40.0% | |
| AACAAATGGATACCTGAGCA+CGG | + | chr2.1:56460681-56460700 | None:intergenic | 40.0% | |
| ATAGGTCATTTGGATGAGAG+CGG | - | chr2.1:56460610-56460629 | MS.gene00309:intron | 40.0% | |
| CACTGAAATAAGTAGGGAAG+TGG | - | chr2.1:56461946-56461965 | MS.gene00309:CDS | 40.0% | |
| CGATGTCATTGTGTTATCTC+TGG | - | chr2.1:56461283-56461302 | MS.gene00309:intron | 40.0% | |
| GAGACCAAAACAAGTAGTTC+TGG | + | chr2.1:56461173-56461192 | None:intergenic | 40.0% | |
| GTTTCAGTTCTGCTACAAAC+AGG | - | chr2.1:56460548-56460567 | MS.gene00309:intron | 40.0% | |
| TACATCACTGATGTCTCTAG+TGG | - | chr2.1:56461799-56461818 | MS.gene00309:intron | 40.0% | |
| TCAGCTGAACATAGGTCATT+TGG | - | chr2.1:56460600-56460619 | MS.gene00309:intron | 40.0% | |
| ACAAATGGATACCTGAGCAC+GGG | + | chr2.1:56460680-56460699 | None:intergenic | 45.0% | |
| ACAGTGGAATTGATCGCCTA+TGG | - | chr2.1:56462080-56462099 | MS.gene00309:intron | 45.0% | |
| AGAATAAGCCCATCCAAACC+AGG | + | chr2.1:56460944-56460963 | None:intergenic | 45.0% | |
| AGCACCATACACACATGTTC+TGG | - | chr2.1:56460821-56460840 | MS.gene00309:intron | 45.0% | |
| AGCTCTACATTCCTAGGAAG+TGG | - | chr2.1:56460439-56460458 | MS.gene00309:CDS | 45.0% | |
| CTTCCTAGGAATGTAGAGCT+CGG | + | chr2.1:56460439-56460458 | None:intergenic | 45.0% | |
| GATGAGAGCGGTGTTTACAA+TGG | - | chr2.1:56460622-56460641 | MS.gene00309:intron | 45.0% | |
| GCACCATACACACATGTTCT+GGG | - | chr2.1:56460822-56460841 | MS.gene00309:intron | 45.0% | |
| GCAGAGGACAAATTCGTCTA+TGG | + | chr2.1:56461210-56461229 | None:intergenic | 45.0% | |
| GCATCAGTTCAGCTGAACAT+AGG | - | chr2.1:56460592-56460611 | MS.gene00309:intron | 45.0% | |
| TCCTCTGCCAGGTCTAAATT+TGG | - | chr2.1:56461222-56461241 | MS.gene00309:intron | 45.0% | |
| TCTAAATTTGGCCTCAGCGA+GGG | - | chr2.1:56461234-56461253 | MS.gene00309:intron | 45.0% | |
| ! | CGGTTTTCTTCTTCTGCCAT+AGG | + | chr2.1:56462099-56462118 | None:intergenic | 45.0% |
| !! | TTCTCCACTTTTGCTCTCTG+TGG | - | chr2.1:56460649-56460668 | MS.gene00309:intron | 45.0% |
| !!! | TTTTTTTATTTTTTATCAAT+TGG | - | chr2.1:56461872-56461891 | MS.gene00309:CDS | 5.0% |
| AGACGAATTTGTCCTCTGCC+AGG | - | chr2.1:56461211-56461230 | MS.gene00309:intron | 50.0% | |
| ATCCAAACCAGGCCTAGACT+GGG | + | chr2.1:56460933-56460952 | None:intergenic | 50.0% | |
| GAAGCCACAGAGAGCAAAAG+TGG | + | chr2.1:56460656-56460675 | None:intergenic | 50.0% | |
| GCCAAATTTAGACCTGGCAG+AGG | + | chr2.1:56461226-56461245 | None:intergenic | 50.0% | |
| GCTGAGGCCAAATTTAGACC+TGG | + | chr2.1:56461232-56461251 | None:intergenic | 50.0% | |
| GTCTAAATTTGGCCTCAGCG+AGG | - | chr2.1:56461233-56461252 | MS.gene00309:intron | 50.0% | |
| TCACCGAGCTCTACATTCCT+AGG | - | chr2.1:56460433-56460452 | MS.gene00309:CDS | 50.0% | |
| TTTCAGGGAGATGCTGACAG+TGG | - | chr2.1:56462064-56462083 | MS.gene00309:intron | 50.0% | |
| !! | AGTCTAGGCCTGGTTTGGAT+GGG | - | chr2.1:56460933-56460952 | MS.gene00309:intron | 50.0% |
| AGTTTCCCTCTGCCCAGTCT+AGG | - | chr2.1:56460918-56460937 | MS.gene00309:intron | 55.0% | |
| CATCCAAACCAGGCCTAGAC+TGG | + | chr2.1:56460934-56460953 | None:intergenic | 55.0% | |
| CTCAATTTGGTCCCTCGCTG+AGG | + | chr2.1:56461248-56461267 | None:intergenic | 55.0% | |
| !! | CAGTCTAGGCCTGGTTTGGA+TGG | - | chr2.1:56460932-56460951 | MS.gene00309:intron | 55.0% |
| TGCCCAGTCTAGGCCTGGTT+TGG | - | chr2.1:56460928-56460947 | MS.gene00309:intron | 60.0% | |
| ACCAGGCCTAGACTGGGCAG+AGG | + | chr2.1:56460927-56460946 | None:intergenic | 65.0% | |
| CCAGGCCTAGACTGGGCAGA+GGG | + | chr2.1:56460926-56460945 | None:intergenic | 65.0% | |
| CTGTGGCTTCACCCGTGCTC+AGG | - | chr2.1:56460666-56460685 | MS.gene00309:intron | 65.0% | |
| CCCTCTGCCCAGTCTAGGCC+TGG | - | chr2.1:56460923-56460942 | MS.gene00309:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 56460411 | 56462132 | 56460411 | ID=MS.gene00309 |
| chr2.1 | mRNA | 56460411 | 56462132 | 56460411 | ID=MS.gene00309.t1;Parent=MS.gene00309 |
| chr2.1 | exon | 56462083 | 56462132 | 56462083 | ID=MS.gene00309.t1.exon1;Parent=MS.gene00309.t1 |
| chr2.1 | CDS | 56462083 | 56462132 | 56462083 | ID=cds.MS.gene00309.t1;Parent=MS.gene00309.t1 |
| chr2.1 | exon | 56461856 | 56461988 | 56461856 | ID=MS.gene00309.t1.exon2;Parent=MS.gene00309.t1 |
| chr2.1 | CDS | 56461856 | 56461988 | 56461856 | ID=cds.MS.gene00309.t1;Parent=MS.gene00309.t1 |
| chr2.1 | exon | 56460411 | 56460473 | 56460411 | ID=MS.gene00309.t1.exon3;Parent=MS.gene00309.t1 |
| chr2.1 | CDS | 56460411 | 56460473 | 56460411 | ID=cds.MS.gene00309.t1;Parent=MS.gene00309.t1 |
| Gene Sequence |
| Protein sequence |
