Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073358.t1 | XP_013463254.1 | 100 | 81 | 0 | 0 | 1 | 81 | 1 | 81 | 5.00E-39 | 169.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073358.t1 | Q3E902 | 85.2 | 81 | 12 | 0 | 1 | 81 | 1 | 81 | 5.8e-36 | 151.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073358.t1 | B7FGU9 | 100.0 | 81 | 0 | 0 | 1 | 81 | 1 | 81 | 3.6e-39 | 169.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049387 | MS.gene073358 | 0.819773 | 9.74E-53 | -1.69E-46 |
| MS.gene050134 | MS.gene073358 | 0.829613 | 4.67E-55 | -1.69E-46 |
| MS.gene050252 | MS.gene073358 | 0.850775 | 1.37E-60 | -1.69E-46 |
| MS.gene050791 | MS.gene073358 | 0.802506 | 5.43E-49 | -1.69E-46 |
| MS.gene052664 | MS.gene073358 | 0.819739 | 9.92E-53 | -1.69E-46 |
| MS.gene053224 | MS.gene073358 | 0.80836 | 3.22E-50 | -1.69E-46 |
| MS.gene057235 | MS.gene073358 | 0.846842 | 1.68E-59 | -1.69E-46 |
| MS.gene057272 | MS.gene073358 | 0.837376 | 5.39E-57 | -1.69E-46 |
| MS.gene057321 | MS.gene073358 | 0.838179 | 3.35E-57 | -1.69E-46 |
| MS.gene057643 | MS.gene073358 | 0.855073 | 8.06E-62 | -1.69E-46 |
| MS.gene058994 | MS.gene073358 | 0.811366 | 7.26E-51 | -1.69E-46 |
| MS.gene059340 | MS.gene073358 | 0.858455 | 8.14E-63 | -1.69E-46 |
| MS.gene060483 | MS.gene073358 | 0.850564 | 1.56E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene023820 | MS.gene073358 | PPI |
| MS.gene27668 | MS.gene073358 | PPI |
| MS.gene049306 | MS.gene073358 | PPI |
| MS.gene52188 | MS.gene073358 | PPI |
| MS.gene073358 | MS.gene00309 | PPI |
| MS.gene073358 | MS.gene023820 | PPI |
| MS.gene52415 | MS.gene073358 | PPI |
| MS.gene004569 | MS.gene073358 | PPI |
| MS.gene013335 | MS.gene073358 | PPI |
| MS.gene23040 | MS.gene073358 | PPI |
| MS.gene06433 | MS.gene073358 | PPI |
| MS.gene30990 | MS.gene073358 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073358.t1 | MTR_2g436800 | 100.000 | 81 | 0 | 0 | 1 | 81 | 1 | 81 | 4.97e-58 | 172 |
| MS.gene073358.t1 | MTR_7g076530 | 93.827 | 81 | 5 | 0 | 1 | 81 | 1 | 81 | 1.73e-55 | 166 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073358.t1 | AT5G27700 | 85.185 | 81 | 12 | 0 | 1 | 81 | 1 | 81 | 9.02e-51 | 154 |
| MS.gene073358.t1 | AT3G53890 | 80.247 | 81 | 16 | 0 | 1 | 81 | 1 | 81 | 1.61e-48 | 148 |
| MS.gene073358.t1 | AT3G53890 | 80.247 | 81 | 16 | 0 | 1 | 81 | 1 | 81 | 1.61e-48 | 148 |
Find 18 sgRNAs with CRISPR-Local
Find 90 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAGCTGAACATAGGTCATT+TGG | 0.363523 | 2.4:-54802438 | MS.gene073358:CDS |
| GTTTCAGTTCTGCTACAAAC+AGG | 0.431981 | 2.4:-54802490 | MS.gene073358:intron |
| CTGTGGCTTCACCCGTGCTC+AGG | 0.453938 | 2.4:-54802372 | MS.gene073358:intron |
| AAAAGTTCATACCACTTCCT+AGG | 0.458041 | 2.4:+54802587 | None:intergenic |
| TCACCGAGCTCTACATTCCT+AGG | 0.480407 | 2.4:-54802604 | MS.gene073358:CDS |
| GAAGCCACAGAGAGCAAAAG+TGG | 0.501571 | 2.4:+54802385 | None:intergenic |
| CTAAAGATGCAGAACGAAGA+AGG | 0.519629 | 2.4:-54802632 | None:intergenic |
| ACAGTGGAATTGATCGCCTA+TGG | 0.527769 | 2.4:-54800939 | MS.gene073358:CDS |
| AGCTCTACATTCCTAGGAAG+TGG | 0.545691 | 2.4:-54802598 | MS.gene073358:intron |
| TTGTGTCTACTGCTTAAGCT+CGG | 0.554847 | 2.4:+54800903 | None:intergenic |
| AAACAGGCTTATTACTGCTA+AGG | 0.565079 | 2.4:-54802474 | MS.gene073358:CDS |
| GCATCAGTTCAGCTGAACAT+AGG | 0.573016 | 2.4:-54802446 | MS.gene073358:CDS |
| GATGAGAGCGGTGTTTACAA+TGG | 0.604206 | 2.4:-54802416 | MS.gene073358:CDS |
| ACAAATGGATACCTGAGCAC+GGG | 0.612936 | 2.4:+54802361 | None:intergenic |
| TTTCAGGGAGATGCTGACAG+TGG | 0.628229 | 2.4:-54800955 | MS.gene073358:intron |
| CTTCCTAGGAATGTAGAGCT+CGG | 0.633169 | 2.4:+54802601 | None:intergenic |
| AACAAATGGATACCTGAGCA+CGG | 0.637714 | 2.4:+54802360 | None:intergenic |
| ATAGGTCATTTGGATGAGAG+CGG | 0.690184 | 2.4:-54802428 | MS.gene073358:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTAAGAAATTATGTTATAT+TGG | + | chr2.4:54801584-54801603 | None:intergenic | 10.0% |
| !!! | AAAATAAGTTTTTAATAAAC+AGG | + | chr2.4:54801949-54801968 | None:intergenic | 10.0% |
| !!! | GTTTATTAAAAACTTATTTT+GGG | - | chr2.4:54801949-54801968 | MS.gene073358:intron | 10.0% |
| !!! | TGTTTATTAAAAACTTATTT+TGG | - | chr2.4:54801948-54801967 | MS.gene073358:intron | 10.0% |
| !! | TATACCAATATAAGATATAT+TGG | + | chr2.4:54801920-54801939 | None:intergenic | 15.0% |
| !! | TATACCAATATATCTTATAT+TGG | - | chr2.4:54801913-54801932 | MS.gene073358:intron | 15.0% |
| !! | TGATTCTATATATCTTATAT+TGG | - | chr2.4:54801854-54801873 | MS.gene073358:intron | 15.0% |
| !! | TTTCATAAGCTAAAAAAATA+AGG | - | chr2.4:54801629-54801648 | MS.gene073358:intron | 15.0% |
| !! | AAGTTTACATATTATCAGTA+TGG | - | chr2.4:54802193-54802212 | MS.gene073358:intron | 20.0% |
| !! | ACTTAAAATTTATACTTCCT+CGG | - | chr2.4:54802027-54802046 | MS.gene073358:intron | 20.0% |
| !! | GAATTCAGAGTAAATAATTA+GGG | - | chr2.4:54801245-54801264 | MS.gene073358:intron | 20.0% |
| !! | TACTGATAATATGTAAACTT+AGG | + | chr2.4:54802193-54802212 | None:intergenic | 20.0% |
| !! | TATAGAGTAGTTATAATATG+CGG | + | chr2.4:54802297-54802316 | None:intergenic | 20.0% |
| !! | TGAATTCAGAGTAAATAATT+AGG | - | chr2.4:54801244-54801263 | MS.gene073358:intron | 20.0% |
| !!! | ACCATTTGTTTCTTATAAAA+AGG | + | chr2.4:54802055-54802074 | None:intergenic | 20.0% |
| !!! | TCCTTTTTATAAGAAACAAA+TGG | - | chr2.4:54802051-54802070 | MS.gene073358:intron | 20.0% |
| ! | AACCAAATACATCAACTTTA+TGG | - | chr2.4:54802109-54802128 | MS.gene073358:intron | 25.0% |
| ! | AGCTAAAAAAATAAGGCAAA+CGG | - | chr2.4:54801636-54801655 | MS.gene073358:intron | 25.0% |
| ! | ATAATATGTAAACTTAGGCA+AGG | + | chr2.4:54802188-54802207 | None:intergenic | 25.0% |
| ! | CTTAAAATTTATACTTCCTC+GGG | - | chr2.4:54802028-54802047 | MS.gene073358:intron | 25.0% |
| ! | GAAAACCTTTATATCTATGT+TGG | - | chr2.4:54802487-54802506 | MS.gene073358:intron | 25.0% |
| ! | TATGTCCAACATAGATATAA+AGG | + | chr2.4:54802495-54802514 | None:intergenic | 25.0% |
| !! | ACGAGTAGTTTTTAATAAAC+AGG | + | chr2.4:54801886-54801905 | None:intergenic | 25.0% |
| !!! | GGTTTCTATCTTTGTTATTT+TGG | - | chr2.4:54801211-54801230 | MS.gene073358:intron | 25.0% |
| !!! | TGATGCTTGTTTTATTTTCA+GGG | - | chr2.4:54802565-54802584 | MS.gene073358:intron | 25.0% |
| !!! | TTGATGCTTGTTTTATTTTC+AGG | - | chr2.4:54802564-54802583 | MS.gene073358:intron | 25.0% |
| !!! | TTTGAACTGTTTTTGTTTTG+TGG | - | chr2.4:54801018-54801037 | MS.gene073358:intron | 25.0% |
| AAAAGAGTGAAGACTCAATT+TGG | + | chr2.4:54801777-54801796 | None:intergenic | 30.0% | |
| AGAAACACACTGAAATAAGT+AGG | - | chr2.4:54802455-54802474 | MS.gene073358:CDS | 30.0% | |
| CATTTGTTCCACTCTAATTT+CGG | - | chr2.4:54801190-54801209 | MS.gene073358:intron | 30.0% | |
| GAAACACACTGAAATAAGTA+GGG | - | chr2.4:54802456-54802475 | MS.gene073358:CDS | 30.0% | |
| GTATTATTCATAAGCTTGTC+AGG | - | chr2.4:54801529-54801548 | MS.gene073358:intron | 30.0% | |
| TAAGAAACAAATGGTTCAAC+AGG | - | chr2.4:54802060-54802079 | MS.gene073358:intron | 30.0% | |
| TATTAATTCATGTCATGCCT+TGG | - | chr2.4:54802522-54802541 | MS.gene073358:intron | 30.0% | |
| TCAAATTTCTCAGCTTACAT+TGG | + | chr2.4:54802253-54802272 | None:intergenic | 30.0% | |
| TCAACAGGAGTTAATGTATT+TGG | - | chr2.4:54802075-54802094 | MS.gene073358:intron | 30.0% | |
| !! | GACCATAAAGTTGATGTATT+TGG | + | chr2.4:54802114-54802133 | None:intergenic | 30.0% |
| AAAACCCAGAAATGTGTGTA+TGG | + | chr2.4:54801345-54801364 | None:intergenic | 35.0% | |
| AAAAGTTCATACCACTTCCT+AGG | + | chr2.4:54800951-54800970 | None:intergenic | 35.0% | |
| AAACAGGCTTATTACTGCTA+AGG | - | chr2.4:54801061-54801080 | MS.gene073358:intron | 35.0% | |
| AAGACAAGCAAAGAAACACA+AGG | + | chr2.4:54801981-54802000 | None:intergenic | 35.0% | |
| AGAGACCAAAACAAGTAGTT+CGG | + | chr2.4:54801690-54801709 | None:intergenic | 35.0% | |
| CGAAATTAGAGTGGAACAAA+TGG | + | chr2.4:54801192-54801211 | None:intergenic | 35.0% | |
| GATAGAAACCGAAATTAGAG+TGG | + | chr2.4:54801201-54801220 | None:intergenic | 35.0% | |
| TTTCTTATAAAAAGGACCCG+AGG | + | chr2.4:54802047-54802066 | None:intergenic | 35.0% | |
| ! | GTTCGGGTTTAATTCTTTGT+CGG | + | chr2.4:54801673-54801692 | None:intergenic | 35.0% |
| ! | TAAACCCGAACTACTTGTTT+TGG | - | chr2.4:54801682-54801701 | MS.gene073358:intron | 35.0% |
| ! | TTCGGGTTTAATTCTTTGTC+GGG | + | chr2.4:54801672-54801691 | None:intergenic | 35.0% |
| !! | ACTCGTCAAAGTATTTTCCA+AGG | + | chr2.4:54802542-54802561 | None:intergenic | 35.0% |
| AACAAATGGATACCTGAGCA+CGG | + | chr2.4:54801178-54801197 | None:intergenic | 40.0% | |
| AAGCACCATACACACATTTC+TGG | - | chr2.4:54801337-54801356 | MS.gene073358:intron | 40.0% | |
| AGCACCATACACACATTTCT+GGG | - | chr2.4:54801338-54801357 | MS.gene073358:intron | 40.0% | |
| ATAGGTCATTTGGATGAGAG+CGG | - | chr2.4:54801107-54801126 | MS.gene073358:intron | 40.0% | |
| CACTGAAATAAGTAGGGAAG+TGG | - | chr2.4:54802462-54802481 | MS.gene073358:CDS | 40.0% | |
| CGATGTCATTGTGTTATCTC+TGG | - | chr2.4:54801799-54801818 | MS.gene073358:intron | 40.0% | |
| GAGACCAAAACAAGTAGTTC+GGG | + | chr2.4:54801689-54801708 | None:intergenic | 40.0% | |
| GTTTCAGTTCTGCTACAAAC+AGG | - | chr2.4:54801045-54801064 | MS.gene073358:intron | 40.0% | |
| TACATCACTGATGTCTCTAG+TGG | - | chr2.4:54802315-54802334 | MS.gene073358:intron | 40.0% | |
| TCAGCTGAACATAGGTCATT+TGG | - | chr2.4:54801097-54801116 | MS.gene073358:intron | 40.0% | |
| ACAAATGGATACCTGAGCAC+GGG | + | chr2.4:54801177-54801196 | None:intergenic | 45.0% | |
| ACAGTGGAATTGATCGCCTA+TGG | - | chr2.4:54802596-54802615 | MS.gene073358:intron | 45.0% | |
| AGAATAAGCCCATCCAAACC+AGG | + | chr2.4:54801460-54801479 | None:intergenic | 45.0% | |
| AGCTCTACATTCCTAGGAAG+TGG | - | chr2.4:54800937-54800956 | MS.gene073358:CDS | 45.0% | |
| CTTCCTAGGAATGTAGAGCT+CGG | + | chr2.4:54800937-54800956 | None:intergenic | 45.0% | |
| GATGAGAGCGGTGTTTACAA+TGG | - | chr2.4:54801119-54801138 | MS.gene073358:intron | 45.0% | |
| GCAGAGGACAAATTCGTCTA+TGG | + | chr2.4:54801726-54801745 | None:intergenic | 45.0% | |
| GCATCAGTTCAGCTGAACAT+AGG | - | chr2.4:54801089-54801108 | MS.gene073358:intron | 45.0% | |
| TCCTCTGCCAGGTCTAAATT+TGG | - | chr2.4:54801738-54801757 | MS.gene073358:intron | 45.0% | |
| TCTAAATTTGGCCTCAGCGA+GGG | - | chr2.4:54801750-54801769 | MS.gene073358:intron | 45.0% | |
| ! | CGGTTTTCTTCTTCTGCCAT+AGG | + | chr2.4:54802615-54802634 | None:intergenic | 45.0% |
| !! | TTCTCCACTTTTGCTCTCTG+TGG | - | chr2.4:54801146-54801165 | MS.gene073358:intron | 45.0% |
| !!! | TTTTTTTATTTTTTATCAAT+TGG | - | chr2.4:54802388-54802407 | MS.gene073358:CDS | 5.0% |
| AGACGAATTTGTCCTCTGCC+AGG | - | chr2.4:54801727-54801746 | MS.gene073358:intron | 50.0% | |
| ATCCAAACCAGGCCTAGACT+GGG | + | chr2.4:54801449-54801468 | None:intergenic | 50.0% | |
| GAAGCCACAGAGAGCAAAAG+TGG | + | chr2.4:54801153-54801172 | None:intergenic | 50.0% | |
| GCCAAATTTAGACCTGGCAG+AGG | + | chr2.4:54801742-54801761 | None:intergenic | 50.0% | |
| GCTGAGGCCAAATTTAGACC+TGG | + | chr2.4:54801748-54801767 | None:intergenic | 50.0% | |
| GTCTAAATTTGGCCTCAGCG+AGG | - | chr2.4:54801749-54801768 | MS.gene073358:intron | 50.0% | |
| TCACCGAGCTCTACATTCCT+AGG | - | chr2.4:54800931-54800950 | MS.gene073358:CDS | 50.0% | |
| TTTCAGGGAGATGCTGACAG+TGG | - | chr2.4:54802580-54802599 | MS.gene073358:intron | 50.0% | |
| !! | AGTCTAGGCCTGGTTTGGAT+GGG | - | chr2.4:54801449-54801468 | MS.gene073358:intron | 50.0% |
| AGTTTCCCTCTGCCCAGTCT+AGG | - | chr2.4:54801434-54801453 | MS.gene073358:intron | 55.0% | |
| CATCCAAACCAGGCCTAGAC+TGG | + | chr2.4:54801450-54801469 | None:intergenic | 55.0% | |
| CTCAATTTGGTCCCTCGCTG+AGG | + | chr2.4:54801764-54801783 | None:intergenic | 55.0% | |
| !! | CAGTCTAGGCCTGGTTTGGA+TGG | - | chr2.4:54801448-54801467 | MS.gene073358:intron | 55.0% |
| TGCCCAGTCTAGGCCTGGTT+TGG | - | chr2.4:54801444-54801463 | MS.gene073358:intron | 60.0% | |
| ACCAGGCCTAGACTGGGCAG+AGG | + | chr2.4:54801443-54801462 | None:intergenic | 65.0% | |
| CCAGGCCTAGACTGGGCAGA+GGG | + | chr2.4:54801442-54801461 | None:intergenic | 65.0% | |
| CTGTGGCTTCACCCGTGCTC+AGG | - | chr2.4:54801163-54801182 | MS.gene073358:intron | 65.0% | |
| CCCTCTGCCCAGTCTAGGCC+TGG | - | chr2.4:54801439-54801458 | MS.gene073358:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 54800909 | 54802648 | 54800909 | ID=MS.gene073358 |
| chr2.4 | mRNA | 54800909 | 54802648 | 54800909 | ID=MS.gene073358.t1;Parent=MS.gene073358 |
| chr2.4 | exon | 54802599 | 54802648 | 54802599 | ID=MS.gene073358.t1.exon1;Parent=MS.gene073358.t1 |
| chr2.4 | CDS | 54802599 | 54802648 | 54802599 | ID=cds.MS.gene073358.t1;Parent=MS.gene073358.t1 |
| chr2.4 | exon | 54802373 | 54802505 | 54802373 | ID=MS.gene073358.t1.exon2;Parent=MS.gene073358.t1 |
| chr2.4 | CDS | 54802373 | 54802505 | 54802373 | ID=cds.MS.gene073358.t1;Parent=MS.gene073358.t1 |
| chr2.4 | exon | 54800909 | 54800971 | 54800909 | ID=MS.gene073358.t1.exon3;Parent=MS.gene073358.t1 |
| chr2.4 | CDS | 54800909 | 54800971 | 54800909 | ID=cds.MS.gene073358.t1;Parent=MS.gene073358.t1 |
| Gene Sequence |
| Protein sequence |
