Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27668.t1 | AES80088.1 | 97.5 | 81 | 2 | 0 | 1 | 81 | 1 | 81 | 2.50E-38 | 167.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27668.t1 | Q3E902 | 86.4 | 81 | 11 | 0 | 1 | 81 | 1 | 81 | 1.2e-36 | 153.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27668.t1 | G7L2J4 | 97.5 | 81 | 2 | 0 | 1 | 81 | 1 | 81 | 1.8e-38 | 167.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049255 | MS.gene27668 | 0.837696 | 4.46E-57 | -1.69E-46 |
| MS.gene049340 | MS.gene27668 | 0.806 | 1.02E-49 | -1.69E-46 |
| MS.gene050277 | MS.gene27668 | 0.815532 | 8.81E-52 | -1.69E-46 |
| MS.gene050916 | MS.gene27668 | 0.811628 | 6.37E-51 | -1.69E-46 |
| MS.gene05134 | MS.gene27668 | 0.811008 | 8.68E-51 | -1.69E-46 |
| MS.gene052879 | MS.gene27668 | 0.820326 | 7.28E-53 | -1.69E-46 |
| MS.gene052880 | MS.gene27668 | 0.808348 | 3.24E-50 | -1.69E-46 |
| MS.gene052947 | MS.gene27668 | 0.827545 | 1.47E-54 | -1.69E-46 |
| MS.gene054937 | MS.gene27668 | 0.808232 | 3.43E-50 | -1.69E-46 |
| MS.gene055912 | MS.gene27668 | 0.810348 | 1.21E-50 | -1.69E-46 |
| MS.gene056631 | MS.gene27668 | 0.814095 | 1.83E-51 | -1.69E-46 |
| MS.gene057705 | MS.gene27668 | 0.835271 | 1.85E-56 | -1.69E-46 |
| MS.gene06001 | MS.gene27668 | 0.811595 | 6.47E-51 | -1.69E-46 |
| MS.gene060429 | MS.gene27668 | 0.831719 | 1.42E-55 | -1.69E-46 |
| MS.gene060917 | MS.gene27668 | 0.823244 | 1.54E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene27668 | MS.gene073358 | PPI |
| MS.gene27668 | MS.gene00309 | PPI |
| MS.gene52415 | MS.gene27668 | PPI |
| MS.gene004569 | MS.gene27668 | PPI |
| MS.gene27668 | MS.gene023820 | PPI |
| MS.gene27668 | MS.gene06433 | PPI |
| MS.gene013335 | MS.gene27668 | PPI |
| MS.gene23040 | MS.gene27668 | PPI |
| MS.gene27668 | MS.gene52188 | PPI |
| MS.gene049306 | MS.gene27668 | PPI |
| MS.gene27668 | MS.gene30990 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27668.t1 | MTR_7g076530 | 97.531 | 81 | 2 | 0 | 1 | 81 | 1 | 81 | 3.25e-57 | 170 |
| MS.gene27668.t1 | MTR_2g436800 | 96.296 | 81 | 3 | 0 | 1 | 81 | 1 | 81 | 5.47e-56 | 167 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27668.t1 | AT5G27700 | 86.420 | 81 | 11 | 0 | 1 | 81 | 1 | 81 | 1.70e-51 | 155 |
| MS.gene27668.t1 | AT3G53890 | 81.481 | 81 | 15 | 0 | 1 | 81 | 1 | 81 | 2.65e-49 | 150 |
| MS.gene27668.t1 | AT3G53890 | 81.481 | 81 | 15 | 0 | 1 | 81 | 1 | 81 | 2.65e-49 | 150 |
Find 15 sgRNAs with CRISPR-Local
Find 85 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTCTACATTCCCAGAAAA+TGG | 0.339849 | 7.2:+29248949 | MS.gene27668:CDS |
| TCAGCTTAACATCGGTCATT+TGG | 0.359324 | 7.2:+29249091 | MS.gene27668:CDS |
| GAATGTAGAGCTCGGTGATT+TGG | 0.379996 | 7.2:-29248938 | None:intergenic |
| CTGTGGCTTCACTCGCGCAC+AGG | 0.458896 | 7.2:+29249157 | MS.gene27668:CDS |
| TACTGCCAAGGATCACGCTT+CGG | 0.492236 | 7.2:+29249067 | MS.gene27668:CDS |
| GAAGCCACAGAGGGCAAAAG+TGG | 0.538865 | 7.2:-29249144 | None:intergenic |
| CTGAACCGAAGCGTGATCCT+TGG | 0.542452 | 7.2:-29249072 | None:intergenic |
| TGCGCGAGTGAAGCCACAGA+GGG | 0.586789 | 7.2:-29249153 | None:intergenic |
| AAACAGATTGATTACTGCCA+AGG | 0.589977 | 7.2:+29249055 | MS.gene27668:CDS |
| GATGAAAGCGGTGTCTACAA+TGG | 0.621212 | 7.2:+29249113 | MS.gene27668:CDS |
| TTCCAGGGAGATGCTGACAG+TGG | 0.628839 | 7.2:+29250402 | MS.gene27668:intron |
| ATCGGTCATTTGGATGAAAG+CGG | 0.639908 | 7.2:+29249101 | MS.gene27668:CDS |
| GCTTCGGTTCAGCTTAACAT+CGG | 0.677252 | 7.2:+29249083 | MS.gene27668:CDS |
| ACAGTGGAATTGATCGCTTG+TGG | 0.696079 | 7.2:+29250418 | MS.gene27668:CDS |
| GTGCGCGAGTGAAGCCACAG+AGG | 0.703435 | 7.2:-29249154 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATAAATCAGAATACAATA+TGG | - | chr7.2:29250280-29250299 | None:intergenic | 15.0% |
| !! | ATCATGAAATAAAGTTAAAA+TGG | + | chr7.2:29249380-29249399 | MS.gene27668:intron | 15.0% |
| !!! | ACATAATTTTAATTAGCTTT+AGG | + | chr7.2:29249331-29249350 | MS.gene27668:intron | 15.0% |
| !!! | TTTATATTTTGTTTAGTATC+TGG | + | chr7.2:29249790-29249809 | MS.gene27668:intron | 15.0% |
| !! | AAATAGTTCCTTAATTAAGA+TGG | + | chr7.2:29249702-29249721 | MS.gene27668:intron | 20.0% |
| !! | ATCTTAATTAAGGAACTATT+TGG | - | chr7.2:29249703-29249722 | None:intergenic | 20.0% |
| !! | ATTCAATACTAATCATGTTT+AGG | + | chr7.2:29249224-29249243 | MS.gene27668:intron | 20.0% |
| !! | TTCAATACTAATCATGTTTA+GGG | + | chr7.2:29249225-29249244 | MS.gene27668:intron | 20.0% |
| !!! | CAAAAAAGAGCTTTTTATAA+GGG | - | chr7.2:29249757-29249776 | None:intergenic | 20.0% |
| !!! | CTTATAAAAAGCTCTTTTTT+GGG | + | chr7.2:29249756-29249775 | MS.gene27668:intron | 20.0% |
| !!! | TTATAAAAAGCTCTTTTTTG+GGG | + | chr7.2:29249757-29249776 | MS.gene27668:intron | 20.0% |
| !!! | TTATGTCATTTTTATTTCCA+GGG | + | chr7.2:29250387-29250406 | MS.gene27668:intron | 20.0% |
| !!! | TTTTATGAATTGCATTTCTA+AGG | + | chr7.2:29250351-29250370 | MS.gene27668:intron | 20.0% |
| ! | AGAATCAACCATCTTAATTA+AGG | - | chr7.2:29249713-29249732 | None:intergenic | 25.0% |
| ! | AGTGAAAAACTAAAAGTGAA+TGG | + | chr7.2:29250037-29250056 | MS.gene27668:intron | 25.0% |
| ! | CAACTAATACTTATAATCTG+CGG | + | chr7.2:29249994-29250013 | MS.gene27668:intron | 25.0% |
| ! | TAATTCCAACGTGTTTATAA+AGG | - | chr7.2:29250248-29250267 | None:intergenic | 25.0% |
| ! | TGAAAATTGATATGTTGCAA+TGG | + | chr7.2:29250098-29250117 | MS.gene27668:intron | 25.0% |
| ! | TGTTGAAAGTTAATGAAGTT+GGG | + | chr7.2:29250197-29250216 | MS.gene27668:intron | 25.0% |
| ! | TTAACAAATTATTATCGACG+TGG | + | chr7.2:29249578-29249597 | MS.gene27668:intron | 25.0% |
| ! | TTTAACCTTTATAAACACGT+TGG | + | chr7.2:29250240-29250259 | MS.gene27668:intron | 25.0% |
| ! | TTTAGTGTAATCATATGTGT+CGG | - | chr7.2:29249548-29249567 | None:intergenic | 25.0% |
| !! | CAAAGTTTATACCATTTTCT+GGG | - | chr7.2:29248963-29248982 | None:intergenic | 25.0% |
| !! | CCAAAAAAGAGCTTTTTATA+AGG | - | chr7.2:29249758-29249777 | None:intergenic | 25.0% |
| !! | TCAAAGTTTATACCATTTTC+TGG | - | chr7.2:29248964-29248983 | None:intergenic | 25.0% |
| !!! | CCTTATAAAAAGCTCTTTTT+TGG | + | chr7.2:29249755-29249774 | MS.gene27668:intron | 25.0% |
| !!! | GTTATGTCATTTTTATTTCC+AGG | + | chr7.2:29250386-29250405 | MS.gene27668:intron | 25.0% |
| !!! | TCTCGTTATTTTCATTGTTA+TGG | + | chr7.2:29248990-29249009 | MS.gene27668:intron | 25.0% |
| ATTGGATTGTGTATTTGTCT+TGG | + | chr7.2:29249255-29249274 | MS.gene27668:intron | 30.0% | |
| GAACAACATAGTATGTTCAA+TGG | - | chr7.2:29250306-29250325 | None:intergenic | 30.0% | |
| GTGTTGAAAGTTAATGAAGT+TGG | + | chr7.2:29250196-29250215 | MS.gene27668:intron | 30.0% | |
| TCATAAAACCTGAATCATCA+AGG | - | chr7.2:29250339-29250358 | None:intergenic | 30.0% | |
| TTGGATTGTGTATTTGTCTT+GGG | + | chr7.2:29249256-29249275 | MS.gene27668:intron | 30.0% | |
| ! | GTTGATTCTGTTTCTCATAA+TGG | + | chr7.2:29249724-29249743 | MS.gene27668:intron | 30.0% |
| !! | CATGTTTAGGGTATTGAAAT+TGG | + | chr7.2:29249237-29249256 | MS.gene27668:intron | 30.0% |
| AAACAGATTGATTACTGCCA+AGG | + | chr7.2:29249055-29249074 | MS.gene27668:CDS | 35.0% | |
| AAGTGAACAAAGAAGGACAA+TGG | + | chr7.2:29249910-29249929 | MS.gene27668:intron | 35.0% | |
| ACTTTCTCATACTCTTCTTG+CGG | - | chr7.2:29250020-29250039 | None:intergenic | 35.0% | |
| ATTGTCCTTCTTTGTTCACT+TGG | - | chr7.2:29249911-29249930 | None:intergenic | 35.0% | |
| GCACAGGTCTCTAATTAATT+TGG | + | chr7.2:29249173-29249192 | MS.gene27668:intron | 35.0% | |
| GTTAATGAAGTTGGGAACTA+TGG | + | chr7.2:29250205-29250224 | MS.gene27668:intron | 35.0% | |
| TCATAGGTTGTGAGACTATT+TGG | - | chr7.2:29249465-29249484 | None:intergenic | 35.0% | |
| TGATTCCAAGTGAACAAAGA+AGG | + | chr7.2:29249903-29249922 | MS.gene27668:intron | 35.0% | |
| TGTCCTGTATTTCATTCTGT+GGG | + | chr7.2:29249825-29249844 | MS.gene27668:intron | 35.0% | |
| TTGTCCTGTATTTCATTCTG+TGG | + | chr7.2:29249824-29249843 | MS.gene27668:intron | 35.0% | |
| ! | TTGATACACCTTGATGATTC+AGG | + | chr7.2:29250328-29250347 | MS.gene27668:intron | 35.0% |
| !!! | GCTCTTTTTTGGGGAAAATT+TGG | + | chr7.2:29249766-29249785 | MS.gene27668:intron | 35.0% |
| ACAACCACTTGCAAAGTTGT+GGG | - | chr7.2:29249652-29249671 | None:intergenic | 40.0% | |
| AGCTCTACATTCCCAGAAAA+TGG | + | chr7.2:29248949-29248968 | MS.gene27668:CDS | 40.0% | |
| ATCGGTCATTTGGATGAAAG+CGG | + | chr7.2:29249101-29249120 | MS.gene27668:CDS | 40.0% | |
| GTCCTGTATTTCATTCTGTG+GGG | + | chr7.2:29249826-29249845 | MS.gene27668:intron | 40.0% | |
| TATTATCGACGTGGACTTGT+CGG | + | chr7.2:29249587-29249606 | MS.gene27668:intron | 40.0% | |
| TCAGCTTAACATCGGTCATT+TGG | + | chr7.2:29249091-29249110 | MS.gene27668:CDS | 40.0% | |
| TTTGCAAGTGGTTGTGTTTG+TGG | + | chr7.2:29249657-29249676 | MS.gene27668:intron | 40.0% | |
| !! | TTTTCTGGGAATGTAGAGCT+CGG | - | chr7.2:29248949-29248968 | None:intergenic | 40.0% |
| ACAGTGGAATTGATCGCTTG+TGG | + | chr7.2:29250418-29250437 | MS.gene27668:CDS | 45.0% | |
| AGCCCCACAGAATGAAATAC+AGG | - | chr7.2:29249831-29249850 | None:intergenic | 45.0% | |
| ATAGCCCACAACTTTGCAAG+TGG | + | chr7.2:29249645-29249664 | MS.gene27668:intron | 45.0% | |
| ATGAAGCACGGAAACAAACC+CGG | - | chr7.2:29249627-29249646 | None:intergenic | 45.0% | |
| CACAACCACTTGCAAAGTTG+TGG | - | chr7.2:29249653-29249672 | None:intergenic | 45.0% | |
| GATGAAAGCGGTGTCTACAA+TGG | + | chr7.2:29249113-29249132 | MS.gene27668:CDS | 45.0% | |
| GCTTCGGTTCAGCTTAACAT+CGG | + | chr7.2:29249083-29249102 | MS.gene27668:CDS | 45.0% | |
| GTCTCACAACCTATGATGCA+CGG | + | chr7.2:29249469-29249488 | MS.gene27668:intron | 45.0% | |
| ! | AAGTTGTGGGCTATGAAGCA+CGG | - | chr7.2:29249639-29249658 | None:intergenic | 45.0% |
| ! | GAATGTAGAGCTCGGTGATT+TGG | - | chr7.2:29248941-29248960 | None:intergenic | 45.0% |
| !! | TTTTCCACTTTTGCCCTCTG+TGG | + | chr7.2:29249140-29249159 | MS.gene27668:CDS | 45.0% |
| TACTGCCAAGGATCACGCTT+CGG | + | chr7.2:29249067-29249086 | MS.gene27668:CDS | 50.0% | |
| TATGATGCACGGACACTCCT+CGG | + | chr7.2:29249480-29249499 | MS.gene27668:intron | 50.0% | |
| ! | GTCGGTGTCTATGTCATGTC+CGG | + | chr7.2:29249605-29249624 | MS.gene27668:intron | 50.0% |
| ! | TCGGTGTCTATGTCATGTCC+GGG | + | chr7.2:29249606-29249625 | MS.gene27668:intron | 50.0% |
| CTGAACCGAAGCGTGATCCT+TGG | - | chr7.2:29249075-29249094 | None:intergenic | 55.0% | |
| GAAGCCACAGAGGGCAAAAG+TGG | - | chr7.2:29249147-29249166 | None:intergenic | 55.0% | |
| GATTAGGTCTGTCCCGGTGT+CGG | + | chr7.2:29249502-29249521 | MS.gene27668:intron | 55.0% | |
| TTCCACTGTCAGCATCTCCC+TGG | - | chr7.2:29250407-29250426 | None:intergenic | 55.0% | |
| TTCCAGGGAGATGCTGACAG+TGG | + | chr7.2:29250402-29250421 | MS.gene27668:intron | 55.0% | |
| ! | TCCTCGGATTAGGTCTGTCC+CGG | + | chr7.2:29249496-29249515 | MS.gene27668:intron | 55.0% |
| !! | GAGGAGTGTCCGTGCATCAT+AGG | - | chr7.2:29249481-29249500 | None:intergenic | 55.0% |
| ACCGGGACAGACCTAATCCG+AGG | - | chr7.2:29249500-29249519 | None:intergenic | 60.0% | |
| GCACGGACACTCCTCGGATT+AGG | + | chr7.2:29249486-29249505 | MS.gene27668:intron | 60.0% | |
| TGCGCGAGTGAAGCCACAGA+GGG | - | chr7.2:29249156-29249175 | None:intergenic | 60.0% | |
| ACCAACGCGTGTCCGACACC+GGG | - | chr7.2:29249517-29249536 | None:intergenic | 65.0% | |
| CACCAACGCGTGTCCGACAC+CGG | - | chr7.2:29249518-29249537 | None:intergenic | 65.0% | |
| CTGTGGCTTCACTCGCGCAC+AGG | + | chr7.2:29249157-29249176 | MS.gene27668:CDS | 65.0% | |
| GTGCGCGAGTGAAGCCACAG+AGG | - | chr7.2:29249157-29249176 | None:intergenic | 65.0% | |
| ! | TCCCGGTGTCGGACACGCGT+TGG | + | chr7.2:29249513-29249532 | MS.gene27668:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 29248921 | 29250470 | 29248921 | ID=MS.gene27668 |
| chr7.2 | mRNA | 29248921 | 29250470 | 29248921 | ID=MS.gene27668.t1;Parent=MS.gene27668 |
| chr7.2 | exon | 29248921 | 29248970 | 29248921 | ID=MS.gene27668.t1.exon1;Parent=MS.gene27668.t1 |
| chr7.2 | CDS | 29248921 | 29248970 | 29248921 | ID=cds.MS.gene27668.t1;Parent=MS.gene27668.t1 |
| chr7.2 | exon | 29249046 | 29249178 | 29249046 | ID=MS.gene27668.t1.exon2;Parent=MS.gene27668.t1 |
| chr7.2 | CDS | 29249046 | 29249178 | 29249046 | ID=cds.MS.gene27668.t1;Parent=MS.gene27668.t1 |
| chr7.2 | exon | 29250408 | 29250470 | 29250408 | ID=MS.gene27668.t1.exon3;Parent=MS.gene27668.t1 |
| chr7.2 | CDS | 29250408 | 29250470 | 29250408 | ID=cds.MS.gene27668.t1;Parent=MS.gene27668.t1 |
| Gene Sequence |
| Protein sequence |
