Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52415.t1 | XP_013463254.1 | 100 | 81 | 0 | 0 | 1 | 81 | 1 | 81 | 5.00E-39 | 169.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52415.t1 | Q3E902 | 85.2 | 81 | 12 | 0 | 1 | 81 | 1 | 81 | 5.8e-36 | 151.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52415.t1 | B7FGU9 | 100.0 | 81 | 0 | 0 | 1 | 81 | 1 | 81 | 3.6e-39 | 169.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049361 | MS.gene52415 | 0.844312 | 8.17E-59 | -1.69E-46 |
| MS.gene050134 | MS.gene52415 | 0.835323 | 1.79E-56 | -1.69E-46 |
| MS.gene050638 | MS.gene52415 | -0.800847 | 1.19E-48 | -1.69E-46 |
| MS.gene050791 | MS.gene52415 | 0.831593 | 1.53E-55 | -1.69E-46 |
| MS.gene051984 | MS.gene52415 | 0.815267 | 1.01E-51 | -1.69E-46 |
| MS.gene052412 | MS.gene52415 | 0.805787 | 1.13E-49 | -1.69E-46 |
| MS.gene052666 | MS.gene52415 | 0.82654 | 2.57E-54 | -1.69E-46 |
| MS.gene057641 | MS.gene52415 | 0.870247 | 1.69E-66 | -1.69E-46 |
| MS.gene057643 | MS.gene52415 | 0.878173 | 3.48E-69 | -1.69E-46 |
| MS.gene060483 | MS.gene52415 | 0.860451 | 2.05E-63 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene52415 | MS.gene52188 | PPI |
| MS.gene52415 | MS.gene27668 | PPI |
| MS.gene52415 | MS.gene30990 | PPI |
| MS.gene52415 | MS.gene23040 | PPI |
| MS.gene52415 | MS.gene06433 | PPI |
| MS.gene52415 | MS.gene073358 | PPI |
| MS.gene52415 | MS.gene00309 | PPI |
| MS.gene52415 | MS.gene023820 | PPI |
| MS.gene52415 | MS.gene004569 | PPI |
| MS.gene049306 | MS.gene52415 | PPI |
| MS.gene52415 | MS.gene013335 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52415.t1 | MTR_2g436800 | 100.000 | 81 | 0 | 0 | 1 | 81 | 1 | 81 | 4.97e-58 | 172 |
| MS.gene52415.t1 | MTR_7g076530 | 93.827 | 81 | 5 | 0 | 1 | 81 | 1 | 81 | 1.73e-55 | 166 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52415.t1 | AT5G27700 | 85.185 | 81 | 12 | 0 | 1 | 81 | 1 | 81 | 9.02e-51 | 154 |
| MS.gene52415.t1 | AT3G53890 | 80.247 | 81 | 16 | 0 | 1 | 81 | 1 | 81 | 1.61e-48 | 148 |
| MS.gene52415.t1 | AT3G53890 | 80.247 | 81 | 16 | 0 | 1 | 81 | 1 | 81 | 1.61e-48 | 148 |
Find 18 sgRNAs with CRISPR-Local
Find 91 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAGCTGAACATAGGTCATT+TGG | 0.363523 | 2.3:-54595269 | MS.gene52415:CDS |
| GTTTCAGTTCTGCTACAAAC+AGG | 0.431981 | 2.3:-54595321 | MS.gene52415:intron |
| CTGTGGCTTCACCCGTGCTC+AGG | 0.453938 | 2.3:-54595203 | MS.gene52415:intron |
| AAAAGTTCATACCACTTCCT+AGG | 0.458041 | 2.3:+54595418 | None:intergenic |
| TCACCGAGCTCTACATTCCT+AGG | 0.480407 | 2.3:-54595435 | MS.gene52415:CDS |
| GAAGCCACAGAGAGCAAAAG+TGG | 0.501571 | 2.3:+54595216 | None:intergenic |
| CTAAAGATGCAGAACGAAGA+AGG | 0.519629 | 2.3:-54595463 | None:intergenic |
| ACAGTGGAATTGATCGCCTA+TGG | 0.527769 | 2.3:-54593771 | MS.gene52415:CDS |
| AGCTCTACATTCCTAGGAAG+TGG | 0.545691 | 2.3:-54595429 | MS.gene52415:intron |
| TTGTGTCTACTGCTTAAGCT+CGG | 0.551699 | 2.3:+54593735 | None:intergenic |
| AAACAGGCTTATTACTGCTA+AGG | 0.565079 | 2.3:-54595305 | MS.gene52415:CDS |
| GCATCAGTTCAGCTGAACAT+AGG | 0.573016 | 2.3:-54595277 | MS.gene52415:CDS |
| GATGAGAGCGGTGTTTACAA+TGG | 0.604206 | 2.3:-54595247 | MS.gene52415:CDS |
| ACAAATGGATACCTGAGCAC+GGG | 0.612936 | 2.3:+54595192 | None:intergenic |
| TTTCAGGGAGATGCTGACAG+TGG | 0.628229 | 2.3:-54593787 | MS.gene52415:intron |
| CTTCCTAGGAATGTAGAGCT+CGG | 0.633169 | 2.3:+54595432 | None:intergenic |
| AACAAATGGATACCTGAGCA+CGG | 0.637714 | 2.3:+54595191 | None:intergenic |
| ATAGGTCATTTGGATGAGAG+CGG | 0.690184 | 2.3:-54595259 | MS.gene52415:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTAAGAAATTATGTTATAT+TGG | + | chr2.3:54594416-54594435 | None:intergenic | 10.0% |
| !!! | AAAATAAGTTTTTAATAAAC+AGG | + | chr2.3:54594781-54594800 | None:intergenic | 10.0% |
| !!! | GTTTATTAAAAACTTATTTT+GGG | - | chr2.3:54594781-54594800 | MS.gene52415:intron | 10.0% |
| !!! | TGTTTATTAAAAACTTATTT+TGG | - | chr2.3:54594780-54594799 | MS.gene52415:intron | 10.0% |
| !! | GAATTAAGAGTAAATAATTA+GGG | - | chr2.3:54594077-54594096 | MS.gene52415:intron | 15.0% |
| !! | TATACCAATATAAGATATAT+TGG | + | chr2.3:54594752-54594771 | None:intergenic | 15.0% |
| !! | TATACCAATATATCTTATAT+TGG | - | chr2.3:54594745-54594764 | MS.gene52415:intron | 15.0% |
| !! | TGAATTAAGAGTAAATAATT+AGG | - | chr2.3:54594076-54594095 | MS.gene52415:intron | 15.0% |
| !! | TGATTCTATATATCTTATAT+TGG | - | chr2.3:54594686-54594705 | MS.gene52415:intron | 15.0% |
| !! | TTTCATAAGCTAAAAAAATA+AGG | - | chr2.3:54594461-54594480 | MS.gene52415:intron | 15.0% |
| !! | AAGTTTACATATTATCAGTA+TGG | - | chr2.3:54595025-54595044 | MS.gene52415:intron | 20.0% |
| !! | ACTTAAAATTTATACTTCCT+CGG | - | chr2.3:54594859-54594878 | MS.gene52415:intron | 20.0% |
| !! | TACTGATAATATGTAAACTT+AGG | + | chr2.3:54595025-54595044 | None:intergenic | 20.0% |
| !! | TGATTTGAATTAAATCATGT+CGG | - | chr2.3:54594657-54594676 | MS.gene52415:intron | 20.0% |
| !!! | ACCATTTGTTTCTTATAAAA+AGG | + | chr2.3:54594887-54594906 | None:intergenic | 20.0% |
| !!! | TCCTTTTTATAAGAAACAAA+TGG | - | chr2.3:54594883-54594902 | MS.gene52415:intron | 20.0% |
| ! | AACCAAATACATCAACTTTA+TGG | - | chr2.3:54594941-54594960 | MS.gene52415:intron | 25.0% |
| ! | AGCTAAAAAAATAAGGCAAA+CGG | - | chr2.3:54594468-54594487 | MS.gene52415:intron | 25.0% |
| ! | ATAATATGTAAACTTAGGCA+AGG | + | chr2.3:54595020-54595039 | None:intergenic | 25.0% |
| ! | CTTAAAATTTATACTTCCTC+GGG | - | chr2.3:54594860-54594879 | MS.gene52415:intron | 25.0% |
| ! | GAAAACCTTTATATCTATGT+TGG | - | chr2.3:54595318-54595337 | MS.gene52415:intron | 25.0% |
| ! | TATGTCCAACATAGATATAA+AGG | + | chr2.3:54595326-54595345 | None:intergenic | 25.0% |
| !! | ACGAGTAGTTTTTAATAAAC+AGG | + | chr2.3:54594718-54594737 | None:intergenic | 25.0% |
| !!! | GGTTTCTATCTTTGTTATTT+TGG | - | chr2.3:54594043-54594062 | MS.gene52415:intron | 25.0% |
| !!! | TGATGCTTGTTTTATTTTCA+GGG | - | chr2.3:54595396-54595415 | MS.gene52415:intron | 25.0% |
| !!! | TTGATGCTTGTTTTATTTTC+AGG | - | chr2.3:54595395-54595414 | MS.gene52415:intron | 25.0% |
| !!! | TTTGAACTGTTTTTGTTTTG+TGG | - | chr2.3:54593850-54593869 | MS.gene52415:intron | 25.0% |
| AAAAGAGTGAAGACTCAATT+TGG | + | chr2.3:54594609-54594628 | None:intergenic | 30.0% | |
| AGAAACACACTGAAATAAGT+AGG | - | chr2.3:54595286-54595305 | MS.gene52415:CDS | 30.0% | |
| CATTTGTTCCACTCTAATTT+CGG | - | chr2.3:54594022-54594041 | MS.gene52415:intron | 30.0% | |
| GAAACACACTGAAATAAGTA+GGG | - | chr2.3:54595287-54595306 | MS.gene52415:CDS | 30.0% | |
| GTATTATTCATAAGCTTGTC+AGG | - | chr2.3:54594361-54594380 | MS.gene52415:intron | 30.0% | |
| TAAGAAACAAATGGTTCAAC+AGG | - | chr2.3:54594892-54594911 | MS.gene52415:intron | 30.0% | |
| TATTAATTCATGTCATGCCT+TGG | - | chr2.3:54595353-54595372 | MS.gene52415:intron | 30.0% | |
| TCAAATTTCTCAGCTTACAT+TGG | + | chr2.3:54595085-54595104 | None:intergenic | 30.0% | |
| TCAACAGGAGTTAATGTATT+TGG | - | chr2.3:54594907-54594926 | MS.gene52415:intron | 30.0% | |
| !! | GACCATAAAGTTGATGTATT+TGG | + | chr2.3:54594946-54594965 | None:intergenic | 30.0% |
| AAAACCCAGAAATGTGTGTA+TGG | + | chr2.3:54594177-54594196 | None:intergenic | 35.0% | |
| AAAAGTTCATACCACTTCCT+AGG | + | chr2.3:54593783-54593802 | None:intergenic | 35.0% | |
| AAACAGGCTTATTACTGCTA+AGG | - | chr2.3:54593893-54593912 | MS.gene52415:intron | 35.0% | |
| AAGACAAGCAAAGAAACACA+AGG | + | chr2.3:54594813-54594832 | None:intergenic | 35.0% | |
| AGAGACCAAAACAAGTAGTT+CGG | + | chr2.3:54594522-54594541 | None:intergenic | 35.0% | |
| CGAAATTAGAGTGGAACAAA+TGG | + | chr2.3:54594024-54594043 | None:intergenic | 35.0% | |
| GATAGAAACCGAAATTAGAG+TGG | + | chr2.3:54594033-54594052 | None:intergenic | 35.0% | |
| TTTCTTATAAAAAGGACCCG+AGG | + | chr2.3:54594879-54594898 | None:intergenic | 35.0% | |
| ! | GTTCGGGTTTAATTCTTTGT+CGG | + | chr2.3:54594505-54594524 | None:intergenic | 35.0% |
| ! | TAAACCCGAACTACTTGTTT+TGG | - | chr2.3:54594514-54594533 | MS.gene52415:intron | 35.0% |
| ! | TTCGGGTTTAATTCTTTGTC+GGG | + | chr2.3:54594504-54594523 | None:intergenic | 35.0% |
| !! | ACTCGTCAAAGTATTTTCCA+AGG | + | chr2.3:54595373-54595392 | None:intergenic | 35.0% |
| !!! | GTCTCTAGTGGAGTATTTTT+TGG | - | chr2.3:54595159-54595178 | MS.gene52415:intron | 35.0% |
| AACAAATGGATACCTGAGCA+CGG | + | chr2.3:54594010-54594029 | None:intergenic | 40.0% | |
| AAGCACCATACACACATTTC+TGG | - | chr2.3:54594169-54594188 | MS.gene52415:intron | 40.0% | |
| AGCACCATACACACATTTCT+GGG | - | chr2.3:54594170-54594189 | MS.gene52415:intron | 40.0% | |
| ATAGGTCATTTGGATGAGAG+CGG | - | chr2.3:54593939-54593958 | MS.gene52415:intron | 40.0% | |
| CACTGAAATAAGTAGGGAAG+TGG | - | chr2.3:54595293-54595312 | MS.gene52415:CDS | 40.0% | |
| CGATGTCATTGTGTTATCTC+TGG | - | chr2.3:54594631-54594650 | MS.gene52415:intron | 40.0% | |
| GAGACCAAAACAAGTAGTTC+GGG | + | chr2.3:54594521-54594540 | None:intergenic | 40.0% | |
| GTTTCAGTTCTGCTACAAAC+AGG | - | chr2.3:54593877-54593896 | MS.gene52415:intron | 40.0% | |
| TACATCACTGATGTCTCTAG+TGG | - | chr2.3:54595147-54595166 | MS.gene52415:intron | 40.0% | |
| TCAGCTGAACATAGGTCATT+TGG | - | chr2.3:54593929-54593948 | MS.gene52415:intron | 40.0% | |
| ACAAATGGATACCTGAGCAC+GGG | + | chr2.3:54594009-54594028 | None:intergenic | 45.0% | |
| ACAGTGGAATTGATCGCCTA+TGG | - | chr2.3:54595427-54595446 | MS.gene52415:intron | 45.0% | |
| AGAATAAGCCCATCCAAACC+AGG | + | chr2.3:54594292-54594311 | None:intergenic | 45.0% | |
| AGCTCTACATTCCTAGGAAG+TGG | - | chr2.3:54593769-54593788 | MS.gene52415:CDS | 45.0% | |
| CTTCCTAGGAATGTAGAGCT+CGG | + | chr2.3:54593769-54593788 | None:intergenic | 45.0% | |
| GATGAGAGCGGTGTTTACAA+TGG | - | chr2.3:54593951-54593970 | MS.gene52415:intron | 45.0% | |
| GCAGAGGACAAATTCGTCTA+TGG | + | chr2.3:54594558-54594577 | None:intergenic | 45.0% | |
| GCATCAGTTCAGCTGAACAT+AGG | - | chr2.3:54593921-54593940 | MS.gene52415:intron | 45.0% | |
| TCCTCTGCCAGGTCTAAATT+TGG | - | chr2.3:54594570-54594589 | MS.gene52415:intron | 45.0% | |
| TCTAAATTTGGCCTCAGCGA+GGG | - | chr2.3:54594582-54594601 | MS.gene52415:intron | 45.0% | |
| ! | CGGTTTTCTTCTTCTGCCAT+AGG | + | chr2.3:54595446-54595465 | None:intergenic | 45.0% |
| !! | TTCTCCACTTTTGCTCTCTG+TGG | - | chr2.3:54593978-54593997 | MS.gene52415:intron | 45.0% |
| !!! | TTTTTTTATTTTTTATCAAT+TGG | - | chr2.3:54595219-54595238 | MS.gene52415:CDS | 5.0% |
| AGACGAATTTGTCCTCTGCC+AGG | - | chr2.3:54594559-54594578 | MS.gene52415:intron | 50.0% | |
| ATCCAAACCAGGCCTAGACT+GGG | + | chr2.3:54594281-54594300 | None:intergenic | 50.0% | |
| GAAGCCACAGAGAGCAAAAG+TGG | + | chr2.3:54593985-54594004 | None:intergenic | 50.0% | |
| GCCAAATTTAGACCTGGCAG+AGG | + | chr2.3:54594574-54594593 | None:intergenic | 50.0% | |
| GCTGAGGCCAAATTTAGACC+TGG | + | chr2.3:54594580-54594599 | None:intergenic | 50.0% | |
| GTCTAAATTTGGCCTCAGCG+AGG | - | chr2.3:54594581-54594600 | MS.gene52415:intron | 50.0% | |
| TCACCGAGCTCTACATTCCT+AGG | - | chr2.3:54593763-54593782 | MS.gene52415:CDS | 50.0% | |
| TTTCAGGGAGATGCTGACAG+TGG | - | chr2.3:54595411-54595430 | MS.gene52415:intron | 50.0% | |
| !! | AGTCTAGGCCTGGTTTGGAT+GGG | - | chr2.3:54594281-54594300 | MS.gene52415:intron | 50.0% |
| AGTTTCCCTCTGCCCAGTCT+AGG | - | chr2.3:54594266-54594285 | MS.gene52415:intron | 55.0% | |
| CATCCAAACCAGGCCTAGAC+TGG | + | chr2.3:54594282-54594301 | None:intergenic | 55.0% | |
| CTCAATTTGGTCCCTCGCTG+AGG | + | chr2.3:54594596-54594615 | None:intergenic | 55.0% | |
| !! | CAGTCTAGGCCTGGTTTGGA+TGG | - | chr2.3:54594280-54594299 | MS.gene52415:intron | 55.0% |
| TGCCCAGTCTAGGCCTGGTT+TGG | - | chr2.3:54594276-54594295 | MS.gene52415:intron | 60.0% | |
| ACCAGGCCTAGACTGGGCAG+AGG | + | chr2.3:54594275-54594294 | None:intergenic | 65.0% | |
| CCAGGCCTAGACTGGGCAGA+GGG | + | chr2.3:54594274-54594293 | None:intergenic | 65.0% | |
| CTGTGGCTTCACCCGTGCTC+AGG | - | chr2.3:54593995-54594014 | MS.gene52415:intron | 65.0% | |
| CCCTCTGCCCAGTCTAGGCC+TGG | - | chr2.3:54594271-54594290 | MS.gene52415:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 54593741 | 54595479 | 54593741 | ID=MS.gene52415 |
| chr2.3 | mRNA | 54593741 | 54595479 | 54593741 | ID=MS.gene52415.t1;Parent=MS.gene52415 |
| chr2.3 | exon | 54595430 | 54595479 | 54595430 | ID=MS.gene52415.t1.exon1;Parent=MS.gene52415.t1 |
| chr2.3 | CDS | 54595430 | 54595479 | 54595430 | ID=cds.MS.gene52415.t1;Parent=MS.gene52415.t1 |
| chr2.3 | exon | 54595204 | 54595336 | 54595204 | ID=MS.gene52415.t1.exon2;Parent=MS.gene52415.t1 |
| chr2.3 | CDS | 54595204 | 54595336 | 54595204 | ID=cds.MS.gene52415.t1;Parent=MS.gene52415.t1 |
| chr2.3 | exon | 54593741 | 54593803 | 54593741 | ID=MS.gene52415.t1.exon3;Parent=MS.gene52415.t1 |
| chr2.3 | CDS | 54593741 | 54593803 | 54593741 | ID=cds.MS.gene52415.t1;Parent=MS.gene52415.t1 |
| Gene Sequence |
| Protein sequence |
